KR20150062390A - Composition for hair growth containing an extract derived from a petiole of Green tea - Google Patents
Composition for hair growth containing an extract derived from a petiole of Green tea Download PDFInfo
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- KR20150062390A KR20150062390A KR1020130146983A KR20130146983A KR20150062390A KR 20150062390 A KR20150062390 A KR 20150062390A KR 1020130146983 A KR1020130146983 A KR 1020130146983A KR 20130146983 A KR20130146983 A KR 20130146983A KR 20150062390 A KR20150062390 A KR 20150062390A
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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Abstract
Description
본 발명은 피부에 안전하면서, 탈모를 감소시키고 모발 생장을 촉진시키며, 안정성이 높은 녹차잎자루 유래 추출물을 함유하는 조성물에 관한 것이다.
The present invention relates to a composition containing safflower-leaf extract which is safe to the skin, reduces hair loss, promotes hair growth, and has high stability.
인체의 모발은 약 10~15만개 정도로, 각각의 모발은 서로 다른 주기를 거쳐 성장, 퇴화를 반복하게 된다. 모발은 성장하는 성장기(Anagen), 성장기가 끝나고 모발의 형태를 유지하면서 대사 과정이 느려지는 퇴행기(Catagen) 및 모유두가 위축되고 모낭이 차츰 위축되어 모근이 위쪽으로 밀려 올라가 모낭이 작아지는 휴지기(Telogen)의 3단계의 주기를 반복하는데, 이와 같은 모발의 주기 및 수명은 영양 상태, 병력, 유전, 체질, 호르몬 분비 또는 노화 등 다양한 조건에 따라 변화할 수 있다.The hair of the human body is about 100 to 150,000, and each hair repeats growth and degeneration through different cycles. The hair grows in the growing anagen, the catagen which slows the metabolism while maintaining the shape of the hair, and the catagen that slows down the papillary and the hair follicle is gradually contracted and the hair follicles are pushed upward, ). The cycle and lifespan of such hair may vary depending on various conditions such as nutritional status, history, genetics, constitution, hormone secretion or aging.
탈모는 자연적 과정들을 통해 발생하거나, 암과 같은 증상들을 경감시키기 위해 고안된 특정 치료 약물의 사용에 의해 화학적으로 촉진되는 문제이다. 이러한 탈모는 부분적 또는 완전한 대머리를 유발하는 모발 재성장의 결여를 수반한다.Hair loss is a problem that occurs through natural processes or is chemically facilitated by the use of certain therapeutic drugs designed to alleviate symptoms such as cancer. This hair loss involves a lack of hair regrowth leading to partial or complete baldness.
최근 환경 오염, 스트레스, 다이어트, 피로, 올바르지 않은 식습관 등으로 인해 탈모증을 가진 현대인이 많아짐에 따라 심각한 정신적 고통을 겪고 있는 남성 또는 여성이 증가하고 있는 상황이다.Recently, as more and more people with alopecia due to environmental pollution, stress, diet, fatigue, and inadequate eating habits, the number of men or women suffering from serious mental suffering is increasing.
한편, 현재 대표적인 탈모 방지제로는 미국 FDA에서 승인받은 프로페시아와 미녹시딜 등이 있다. 이러한 약물은 임상적으로 분명한 효과를 보이나 성기능 장애 및 피부 자극 등 부작용을 나타낼 뿐만 아니라 여성은 사용이 불가되었거나 혹은 제한적인 단점이 있다. 최근 이러한 약물의 단점을 보완할 수 있는 새로운 기전의 발모제와 더불어 안전하면서 성별에 제한없이 사용할 수 있는 천연물 원료 개발이 활발하게 이루어지고 있다.
On the other hand, there are prophylactic and minoxidil approved by the US FDA as a typical hair loss preventing agent. These drugs have clinically obvious effects, but they also have side effects such as sexual dysfunction and skin irritation, as well as disadvantages that women are unavailable or limited. In recent years, along with a new mechanism for supplementing the disadvantages of these drugs, there has been actively developed a raw material for natural materials which can be safely used regardless of gender.
이에 본 발명자들은 기존에 활용되지 않고 버려졌던 녹차잎자루의 추출물이 피부에 자극이 없으면서 우수한 탈모 예방 및 모발 생장 촉진 효과를 제공할 수 있음을 발견하고 본 발명을 완성하게 되었다.Accordingly, the inventors of the present invention have found that the extract of green tea leafstalk that has not been used before and can be discarded can provide superior hair loss prevention and hair growth promoting effect while having no irritation to the skin, thus completing the present invention.
따라서, 본 발명은 녹차잎자루 유래 추출물을 사용함으로써 우수한 탈모 예방 및 모발 생장 촉진 효과를 나타내는 조성물을 제공하는 것을 목적으로 한다.
Accordingly, it is an object of the present invention to provide a composition that exhibits excellent hair loss prevention and hair growth promoting effect by using an extract derived from green tea leafstalk.
상기한 목적을 달성하기 위하여, 본 발명은 녹차잎자루 유래 추출물을 유효성분으로 함유하는 탈모 예방 또는 모발 생장 촉진용 피부 외용제 조성물을 제공한다.
In order to achieve the above object, the present invention provides a composition for preventing hair loss or promoting hair growth comprising an extract derived from green tea leafstalk as an active ingredient.
본 발명은 기존에 활용되지 못하고 폐기되었던 녹차잎자루를 이용함으로써 폐자원의 활용 측면에서 우수한 이용 가치가 있다. 또한, 본 발명의 조성물은 녹차잎자루 유래 추출물을 함유함으로써 피부에 안전하면서도 제형 안정성이 높으며, 스트레스를 억제하고 모근을 강화시켜 탈모를 예방하면서 모발의 생장을 촉진시킨다.
The present invention has excellent utility value in terms of utilization of waste resources by using green tea leafstock that has not been used in the past and has been discarded. In addition, the composition of the present invention contains a green tea leaf extract-derived extract, which is safe to the skin and has high formulation stability, suppresses stress and strengthens the hair follicle, thereby promoting hair growth while preventing hair loss.
본 발명의 조성물은 녹차잎자루 추출물을 유효성분으로서 함유하여, 우수한 탈모 예방 또는 모발 생장 촉진 효과를 제공할 수 있다.The composition of the present invention contains green tea petiole extract as an active ingredient, thereby providing excellent hair loss prevention or hair growth promoting effect.
녹차(Green tea)는 일반적으로 차나무(Camellia sinensis)의 잎을 이용하여 왔다. 그러나, 본 발명에서는 차나무의 종류 및 재배 일수에는 제한이 없으나, 사용 부위는 잎자루인 것을 특징으로 한다. 또한, 본 발명에서 사용되는 잎자루는 말리거나 말리지 않은 것을 모두 사용할 수 있다.Green tea has generally used leaves of tea (Camellia sinensis). However, in the present invention, there is no limitation on the type of tea and the number of days of cultivation, but the use site is petiole. The petiole used in the present invention may be either dried or not dried.
본 발명에서 사용되는 녹차의 잎자루는 엽병이라고도 하는 부위로서, 잎자루 속의 관다발은 줄기의 관다발과 잎몸의 관다발을 연락하는 역할을 하며, 기존에는 차나무에서 잎을 채취한 후 버려지는 부위였다. 그러나, 본 발명자들이 녹차잎자루를 분석한 결과, 녹차잎과는 포함된 성분의 종류 및 함량에 있어서 큰 차이를 나타내어 녹차잎과는 제공하는 효과에 있어서 차이가 있을 것임을 예측할 수 있었다.The petiole of the green tea used in the present invention is also called a petiole, and the vena cava in the petiole is in contact with the vena cava of the stem and the vena cava of the leaf blade. In the past, the leaf was collected from the tea leaves and then discarded. However, the present inventors analyzed the green tea leafstalk, and found that there was a large difference in the kind and content of the components contained in the green tea leaves, so that it could be predicted that there would be differences in the effect provided by the green tea leaves.
또한, 기존에는 활용되지 못하고 폐기되었던 녹차잎자루 유래의 추출물을 제조하고 이를 상품에 적용함으로써, 고부가가치의 기능성 소재 및 응용제품으로의 이용이 가능하게 되므로, 본 발명은 폐자원의 활용 측면에서도 우수한 이용 가치가 있다.In addition, since the extract of green tea leafstalk which has not been used before but has been discarded can be prepared and applied to the product, it can be used as high value-added functional materials and application products. Therefore, It is worth it.
본 발명에서 사용되는 녹차잎자루 유래 추출물은 당업계에 공지된 방법에 의해 제조될 수 있으며, 그 방법이 특별히 한정되지는 않지만, 바람직하게는 녹차잎자루에 포함된 수용성 성분을 추출하는 방법을 이용한다.The green tea leafstarch-derived extract used in the present invention can be prepared by a method known in the art, and the method thereof is not particularly limited, but preferably a method of extracting a water-soluble component contained in green tea leafstalk is used.
구체적으로, 건조된 녹차잎자루에 녹차잎자루 중량 대비 5배의 열수(온도가 80~90℃인 정제수)를 가하고, 온도를 유지하면서 2시간 가열 교반하고 상온으로 온도를 식힌 후에, 이를 1차 여과하여 수용성 성분만 분리한다. 여과액 전체 중량 대비 2배에 해당하는 에탄올을 천천히 교반하면서 첨가하고, 상등액의 클로로필은 제거하고, 침전된 것만 모아 열풍 건조한다. 열풍 건조시 30~40℃를 유지시켜주어, 열변성을 방지한다. 이러한 방법으로 본 발명에서 사용되는 녹차잎자루 유래 추출물을 제조할 수 있다.Specifically, 5 times of hot water (purified water having a temperature of 80 to 90 ° C) relative to the weight of green tea petiole was added to the dried green tea petiole, and the mixture was heated and stirred for 2 hours while maintaining the temperature. Separate only water-soluble components. Ethanol corresponding to twice the total weight of the filtrate was added with gentle stirring, chlorophyll of the supernatant was removed, and only the precipitate was collected and hot-air dried. It is kept at 30 ~ 40 ℃ during hot air drying to prevent thermal denaturation. In this way, the green tea leaf extract derived from the present invention can be prepared.
이와 같은 방법으로 제조한 녹차잎자루 유래 추출물은 주요 성분이 당단백질이고, 카테킨을 0.90%, 카페인을 0.12% 함유하여, 카테킨을 22.1%, 카페인을 4.61%로 함유하는 일반 녹차잎 추출물과는 그 구성 성분이 상이하며, 이에 의하여 나타내는 효과도 서로 상이하다.The green tea leaf extract derived from such a method has a composition of glycoprotein containing 0.90% of catechin and 0.12% of caffeine, 22.1% of catechin and 4.61% of caffeine, The components are different, and the effects exhibited thereby are also different from each other.
본 발명의 조성물은 녹차잎자루 유래 추출물을 조성물 총 중량에 대하여 0.001~20중량%의 양으로 함유할 수 있다. 이는 상기 유효성분의 함량이 0.001중량% 미만일 경우에는 효능, 효과가 미약하고, 20중량%를 초과할 경우에는 원료 사용량에 따른 효과 증대를 기대하기 어렵다.The composition of the present invention may contain an amount of the green tea petiole extract in an amount of 0.001 to 20% by weight based on the total weight of the composition. If the content of the active ingredient is less than 0.001% by weight, the efficacy and efficacy are insufficient. If the content is more than 20% by weight, it is difficult to expect an increase in the effect according to the amount of raw material.
본 발명에서 사용되는 녹차잎자루 유래 추출물은 스트레스에 의해 정상 상태보다 모낭이 성장기에서 퇴행기로 가속화되는 것을 효과적으로 저해하여 스트레스에 의한 탈모 현상(퇴행기 이행)을 억제하며, 모근을 강화시킴으로써 모발 생장 촉진 효과를 제공하고, 피부에 안전하고 안정도가 높아 산업적으로 이용하기에 바람직하다.The green tea leaf extract derived from green tea used in the present invention effectively inhibits the acceleration of the hair follicle from the growing stage to the regenerating stage rather than the normal state due to the stress, thereby suppressing stress-induced hair loss (regressor migration) and enhancing the hair growth promoting effect And is safe for the skin and has high stability, which is preferable for industrial use.
본 발명의 모발 생장 촉진용 조성물은 제형화하는데 있어서 특별히 한정되는 바가 없으나, 헤어토닉, 모발 영양화장수, 스칼프트리트먼트, 헤어트리트먼트, 헤어린스, 헤어샴푸, 헤어로션 등의 제형을 갖는 화장료 조성물일 수 있다.The composition for promoting hair growth of the present invention is not particularly limited in its formulation, but may be a cosmetic composition having a formulation such as a hair tonic, a hair nutritional lotion, a scarf treatment, a hair treatment, a hair rinse, a hair shampoo, .
또한, 각각의 제형에 있어서 상기한 필수성분 이외에 다른 성분들은 기타 외용제의 종류 또는 사용목적 등에 따라 당업자가 어려움 없이 적합하게 선정하여 배합할 수 있다.
In addition, in the respective formulations, the ingredients other than the above-mentioned essential ingredients can be suitably selected and blended by those skilled in the art depending on the kind of the external preparation, the purpose of use, and the like.
이하, 실시예 및 시험예를 들어 본 발명의 보다 구체적으로 설명하지만, 본 발명이 이들 예로만 한정되는 것은 아니다.
Hereinafter, the present invention will be described in more detail with reference to examples and test examples, but the present invention is not limited to these examples.
[실시예 1] 녹차잎자루 유래 추출물의 제조[Example 1] Preparation of extract from green tea leafstalk
건조된 녹차잎자루(농업법인 장원)에 온도가 80~90℃인 정제수를 녹차잎자루 중량 대비 5배의 양으로 가하고, 온도를 유지하면서 2시간 가열 교반하였다. 상온으로 온도를 식힌 후에, Adventec No. 131(Adventec MFS, Inc., 일본)을 이용하여 1차 여과함으로써 수용성 성분만 분리하였다. 여과액 전체 중량 대비 2배에 해당하는 에탄올을 천천히 교반하면서 첨가하고, 상등액의 클로로필은 제거하고, 침전된 것만 모아, 30~40℃의 온도를 유지하면서 열풍 건조시켜, 녹차잎자루 유래 추출물을 건조된 녹차잎 자루의 1~3중량%의 백색 파우더 형태로 수득하였다.
Purified water having a temperature of 80 to 90 占 폚 was added in an amount of 5 times as much as the weight of the green tea petiole to the dried green tea leafstalk (Miraikan), and the mixture was heated and stirred for 2 hours while maintaining the temperature. After the temperature was cooled to room temperature, Adventec No. 2 was added. 131 (Adventec MFS, Inc., Japan) to separate only water-soluble components. Ethanol corresponding to twice the weight of the total weight of the filtrate was slowly added with stirring, the chlorophyll of the supernatant liquid was removed, and the precipitated solids were collected and subjected to hot air drying while maintaining the temperature at 30 to 40 ° C to extract the green tea leaf extract- 1 to 3% by weight of the green tea leaf sack in the form of a white powder.
[비교예 1] 녹차잎 추출물의 제조[Comparative Example 1] Preparation of green tea leaf extract
시중에서 구입가능한 녹차잎((주) 아모레퍼시픽)에 온도가 80~90℃인 정제수를 녹차잎 중량 대비 5배의 양으로 가하고, 온도를 유지하면서 2시간 가열 교반하였다. 상온으로 온도를 식힌 후에, Adventec No. 131(Adventec MFS, Inc., 일본)을 이용하여 1차 여과함으로써 수용성 성분만 분리하였다. 여과후 감압 증류장치를 이용하여 농축한 후 동결건조기를 이용함으로써 건조시켜, 녹차잎 추출물을 노란갈색 파우더로서 수득하였다.
Purified water having a temperature of 80 to 90 ° C was added to commercially available green tea leaves (AMOREPACIFIC Co., Ltd.) in an amount of 5 times as much as the weight of green tea leaves, and the mixture was heated and stirred for 2 hours while maintaining the temperature. After the temperature was cooled to room temperature, Adventec No. 2 was added. 131 (Adventec MFS, Inc., Japan) to separate only water-soluble components. Filtered, concentrated using a vacuum distillation apparatus, and dried using a freeze dryer to obtain a green tea leaf extract as a yellowish brown powder.
[시험예 1] 탈모 억제 효능 평가[Test Example 1] Estimation of hair loss inhibition efficacy
스트레스는 신경 말단에서 물질 P(Substance P)의 분비를 유도하여 물질 P수용체(NK-1 receptor)를 통하여 대식세포(macrophage), γδT 세포 및 비만세포(mast cell)로 하여금 염증성 신호전달물질(cytokine)을 생산하도록 하여 결과적으로 모낭 내 각질형성세포(keratinocyte)의 성장을 막고 사멸(apoptosis)을 유도하므로(Arck et al.,FASEB J. 2001; 15(13): 2536-2538), 스트레스에 의한 탈모를 방지하기 위하여 기질 P 수용체에 대한 길항제나 스트레스에 따른 일련의 비만세포의 탈과립(degranulation)을 막는 방법 등이 제기되고 있다(Am J Pathol. 2003; 162(3): 709-712).Stress induces the secretion of Substance P from the nerve endings and causes macrophages, γδ T cells and mast cells to pass through the substance P receptor (NK-1 receptor) to the inflammatory signaling substance (cytokine (FASEB J. 2001; 15 (13): 2536-2538). In addition, it has been shown that the production of keratinocytes in hair follicles can be inhibited by stress and by inhibiting the growth of keratinocytes and inducing apoptosis (Am J Pathol. 2003; 162 (3): 709-712) have been proposed to prevent degranulation of a series of mast cells due to antagonists or stress on the substrate P receptor in order to prevent hair loss.
따라서, 본 시험에서는 녹차잎자루 유래 추출물이 비만세포의 탈과립화 정도에 미치는 영향을 측정하였는데, 알러지 즉시반응의 지표인 탈과립에 대한 억제효과를 살펴 보기 위하여 β-헥소사미니다제(β-hexosaminidase)의 분비를 측정하였다. Therefore, in this test, the effect of green tea leafstock-derived extract on the degree of degranulation of mast cells was measured. In order to examine the inhibitory effect on degranulation, which is an indicator of immediate response to allergic reaction, β-hexosaminidase Secretion was measured.
비만세포(RBL-2H3 세포)를 10% FBS를 포함한 DMEM에 현탁시킨 후 48 웰 플레이트(Corning, NY, USA)에 5 × 105 세포수/㎖의 양으로 세포를 분주하였다. 그 후 항-DNP IgE(30ng/㎖)로 감작하고 37℃ 5% CO2 인큐베이터에서 24시간 배양하였다. 각 웰의 세포들을 시라가니언 버퍼(Siraganian buffer)(119mM NaCl, 5mM KCl, 0.4mM MgCl2, 25mM PIPES, 40mM NaOH, pH 7.2)로 2 번 세척한 다음 각 웰당 5.6mM 글루코스, 1mM CaCl2와 0.1% BSA가 포함된 시라가니언 버퍼를 첨가하고 상기 실시예 1의 녹차잎자루 추출물과 비교예 1의 녹차잎 추출물을 농도별(0, 50, 100ppm)로 1시간 동안 37℃ 5% CO2 인큐베이터에서 배양한 후 DNP-HSA(10㎍/㎖)로 처리하여 1시간 동안 반응시키고, 빙욕(ice bath)에서 10분 동안 방치한 후 반응을 종결시켰다. 상층액 20μl를 96웰 플레이트에 옮기고 기질 버퍼(4-p-니트로페닐-N-아세틸-b-D-글루코사미니드 1mM, 시트르산나트륨 0.05M, pH 4.5) 80μl를 넣고 37℃에서 30분 배양시킨 다음 각 웰 당 정지 용액(0.1M Na2CO3/NaHCO3, pH 10.0) 200μl를 첨가하여 반응을 종결시켰다. 마이크로플레이트 판독기(Molecular Devices Co. Ltd., USA)를 사용하여 405nm에서 흡광도를 측정하였다. 녹차잎자루 추출물과 녹차잎 추출물 처리군과 대조군의 흡광도 값으로 하기 수학식 1에 의해 억제율(%)을 산출하였다.The mast cells (RBL-2H3 cells) were suspended in DMEM containing 10% FBS, and then the cells were divided into 48 well plates (Corning, NY, USA) in an amount of 5 × 10 5 cells / ml. The cells were then sensitized with anti-DNP IgE (30 ng / ml) and cultured in a 37 ° C 5% CO 2 incubator for 24 hours. Cells of each well were washed twice with Siraganian buffer (119 mM NaCl, 5 mM KCl, 0.4 mM MgCl 2 , 25 mM PIPES, 40 mM NaOH, pH 7.2), and then 5.6 mM glucose, 1 mM CaCl 2 The shiraganian buffer containing 0.1% BSA was added and the green tea leaf extract of Example 1 and the green tea leaf extract of Comparative Example 1 were incubated for 1 hour at a concentration of 0, 50, 100 ppm in a 37 ° C 5% CO 2 incubator , Treated with DNP-HSA (10 μg / ml) for 1 hour, allowed to stand in an ice bath for 10 minutes, and then terminated. 20 μl of the supernatant was transferred to a 96-well plate, and 80 μl of a substrate buffer (1 mM 4-p-nitrophenyl-N-acetyl-bD-glucosaminide, sodium citrate 0.05M, pH 4.5) was added and incubated at 37 ° C for 30 minutes stop solution per well (0.1M Na 2 CO 3 / NaHCO 3, pH 10.0) and the reaction was terminated by the addition of 200μl. Absorbance was measured at 405 nm using a microplate reader (Molecular Devices Co. Ltd., USA). The inhibition rate (%) was calculated by the following equation (1) as the absorbance value of green tea leafstalk extract and green tea leaf extract treated group and control group.
C: 세포 (+), DNP-HSA (+), 시험 샘플 (-)C: cells (+), DNP-HSA (+), test sample (-)
T: 세포 (+), DNP-HSA (+), 시험 샘플 (+)T: cells (+), DNP-HSA (+), test sample (+)
측정 결과는 하기 표 1에 나타내었다.The measurement results are shown in Table 1 below.
상기 표 1을 보면, 녹차잎 추출물인 비교예 1을 처리한 경우에는 비만세포의 탈과립화를 억제하기는 하지만 그 정도가 크지 않은 반면, 녹차잎자루 추출물인 실시예 1을 처리한 경우에는 비만세포의 탈과립화를 효과적으로 억제한다는 것을 확인할 수 있다.Table 1 shows that the treatment of Comparative Example 1, which is a green tea leaf extract, inhibited the degranulation of mast cells, but the degree of degranulation was not so high. On the other hand, in Example 1 of the green tea leaf extract, It can be confirmed that degranulation is effectively suppressed.
이를 통해 본 발명에서 사용하는 녹차잎자루 추출물은 세포내 염증물질의 생성을 차단하고, 따라서 스트레스에 의한 탈모를 방지하는데 효과적으로 작용할 수 있음을 알 수 있다.
Thus, it can be seen that the green tea petiole extract used in the present invention blocks the production of intracellular inflammatory substances and thus can effectively prevent hair loss due to stress.
[시험예 2] 피부 안정성 평가[Test Example 2] Evaluation of skin stability
본 발명에서 사용하는 녹차잎자루 추출물이 피부에 자극을 미치는 정도를 알아보기 위하여, 인체 피부 일차 자극 시험을 진행하였다.In order to examine the degree of stimulation of the skin of the green tea petiole extract used in the present invention, a human skin primary stimulation test was conducted.
시험은 2회에 걸쳐서 진행하였으며, 1회차에는 건강한 성인 여성 34명, 남성 6명 총 40명(평균 나이 35.1세)을 대상으로 하였고, 2회차에는 여성 28명, 남성 2명 총 30명(평균 나이 30.1세)을 대상으로 하였다.The study consisted of 34 healthy adult women and 6 men (mean age 35.1 years) in the first round. In the second round, 28 women and 2 men totaled 30 Age 30.1 years) were included in this study.
구체적으로 상기 실시예 1에서 수득한 녹차잎자루 추출물을 물에 용해시켜 10%의 농도로 만든 용액을 20mg/cm2의 양으로 등 부위의 피부에 고르게 도포하였다.Specifically, a solution prepared by dissolving the green tea leaf extract obtained in Example 1 in water to a concentration of 10% was applied evenly to the skin of the back region in an amount of 20 mg / cm 2 .
도포 후 그 부위에 첩포를 부착하고, 24시간 경과 후 첩포를 제거한 다음 30분 후에 첫 판독을 시행하였고, 24시간이 경과한 후에 2차 판독을 시행하였다. 1+ 이상인 경우에 양성으로 판독하였으며 마지막 판독을 확정적인 것으로 간주하였다. 시료의 피부자극의 강도를 알아보기 위해 피부의 양성 반응의 정도에 따라 가중치를 부여(-, +, ++, +++, ++++ 에 각각 0, 1, 2, 3, 4의 수치를 부여)하여 피부의 평균반응도를 구하였다. 구체적인 판정 기준은 하기 표 2에 제시되어 있다. 또한, 피부 평균 반응도 산출은 하기 수학식 2에 의해 계산하였으며, 이에 따른 결과 판정 기준은 표 3에 제시되어 있다.After the application, the patch was attached to the area, and after 24 hours, the patch was removed, and the first reading was performed 30 minutes later and the second reading was performed after 24 hours. 1+ was positive and the final reading was considered definitive. To determine the intensity of the skin irritation of the sample, weights were assigned according to the degree of positive skin reaction (-, +, ++, +++, ++++, 0, 1, 2, 3, 4 To give an average response of the skin. Specific criteria are given in Table 2 below. In addition, the calculation of the skin averaged response was calculated by the following equation (2), and the results are shown in Table 3 below.
1.0 ~ 2.9
3.0 ~ 4.9
5.0 이상0.0 to 0.9
1.0 to 2.9
3.0 ~ 4.9
5.0 or higher
경자극
중자극
강자극No stimulation
Light stimulus
Moderate irritation
River stimulation
측정 결과 녹차잎자루 추출물을 사용한 후의 피부 평균 반응도는 0.0~0.9 사이인 것으로 나와 피부에 대하여 자극이 없는 것으로 최종 판정되었다.
The results were as follows: Average skin response was 0.0 ~ 0.9 after using green tea leafstalk extract.
이하, 본 발명에 따른 조성물의 제형예를 설명하나, 약학 조성물 및 화장료 조성물은 여러 가지 제형으로 응용 가능하며, 이는 본 발명을 한정하고자 함이 아닌 단지 구체적으로 설명하고자 함이다.
Hereinafter, a formulation example of the composition according to the present invention will be described. However, the pharmaceutical composition and the cosmetic composition can be applied to various formulations, which are not intended to limit the present invention but merely to illustrate the present invention.
[제형예 1] 헤어토닉[Formulation Example 1] Hair tonic
하기 표 4에 기재된 조성에 따라 통상적인 방법으로 헤어토닉을 제조하였다.Hair tonics were prepared according to the compositions shown in Table 4 by the conventional method.
[제형예 2] 헤어로션[Formulation Example 2] Hair lotion
하기 표 5에 기재된 조성에 따라 통상적인 방법으로 헤어로션을 제조하였다.Hair lotions were prepared in a conventional manner according to the composition shown in Table 5 below.
[제형예 3] 모발 영양화장수[Formulation Example 3] Hair nutrition lotion
하기 표 6에 기재된 조성에 따라 통상적인 방법으로 모발 영양화장수를 제조하였다.Hair nutritional lotion was prepared according to a conventional method according to the composition shown in Table 6 below.
[제형예 4] 헤어샴푸 [Formulation Example 4] Hair shampoo
하기 표 7에 기재된 조성에 따라 통상적인 방법으로 헤어샴푸를 제조하였다.Hair shampoos were prepared in a conventional manner according to the composition shown in Table 7 below.
[제형예 5] 헤어린스[Formulation Example 5] Hair conditioner
하기 표 8에 기재된 조성에 따라 통상적인 방법으로 헤어린스를 제조하였다.The hair rinse was prepared according to a conventional method according to the composition shown in Table 8 below.
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