KR20150062390A - Composition for hair growth containing an extract derived from a petiole of Green tea - Google Patents

Abstract

The present invention relates to a composition containing an extract derived from a petiole of a green tea leaf. The composition is safe to skin, reduces hair loss, promotes hair growth, and is highly stable. The composition contains 0.001-20 wt% of the extract with respect to the total weight thereof.

Description

TECHNICAL FIELD The present invention relates to a composition for promoting hair growth comprising an extract derived from green tea leafstalk,

The present invention relates to a composition containing safflower-leaf extract which is safe to the skin, reduces hair loss, promotes hair growth, and has high stability.

The hair of the human body is about 100 to 150,000, and each hair repeats growth and degeneration through different cycles. The hair grows in the growing anagen, the catagen which slows the metabolism while maintaining the shape of the hair, and the catagen that slows down the papillary and the hair follicle is gradually contracted and the hair follicles are pushed upward, ). The cycle and lifespan of such hair may vary depending on various conditions such as nutritional status, history, genetics, constitution, hormone secretion or aging.

Hair loss is a problem that occurs through natural processes or is chemically facilitated by the use of certain therapeutic drugs designed to alleviate symptoms such as cancer. This hair loss involves a lack of hair regrowth leading to partial or complete baldness.

Recently, as more and more people with alopecia due to environmental pollution, stress, diet, fatigue, and inadequate eating habits, the number of men or women suffering from serious mental suffering is increasing.

On the other hand, there are prophylactic and minoxidil approved by the US FDA as a typical hair loss preventing agent. These drugs have clinically obvious effects, but they also have side effects such as sexual dysfunction and skin irritation, as well as disadvantages that women are unavailable or limited. In recent years, along with a new mechanism for supplementing the disadvantages of these drugs, there has been actively developed a raw material for natural materials which can be safely used regardless of gender.

Korean Laid-Open Publication No. 2013-0108854 (Published Oct. 10, 2013)

Accordingly, the inventors of the present invention have found that the extract of green tea leafstalk that has not been used before and can be discarded can provide superior hair loss prevention and hair growth promoting effect while having no irritation to the skin, thus completing the present invention.

Accordingly, it is an object of the present invention to provide a composition that exhibits excellent hair loss prevention and hair growth promoting effect by using an extract derived from green tea leafstalk.

In order to achieve the above object, the present invention provides a composition for preventing hair loss or promoting hair growth comprising an extract derived from green tea leafstalk as an active ingredient.

The present invention has excellent utility value in terms of utilization of waste resources by using green tea leafstock that has not been used in the past and has been discarded. In addition, the composition of the present invention contains a green tea leaf extract-derived extract, which is safe to the skin and has high formulation stability, suppresses stress and strengthens the hair follicle, thereby promoting hair growth while preventing hair loss.

The composition of the present invention contains green tea petiole extract as an active ingredient, thereby providing excellent hair loss prevention or hair growth promoting effect.

Green tea has generally used leaves of tea (Camellia sinensis). However, in the present invention, there is no limitation on the type of tea and the number of days of cultivation, but the use site is petiole. The petiole used in the present invention may be either dried or not dried.

The petiole of the green tea used in the present invention is also called a petiole, and the vena cava in the petiole is in contact with the vena cava of the stem and the vena cava of the leaf blade. In the past, the leaf was collected from the tea leaves and then discarded. However, the present inventors analyzed the green tea leafstalk, and found that there was a large difference in the kind and content of the components contained in the green tea leaves, so that it could be predicted that there would be differences in the effect provided by the green tea leaves.

In addition, since the extract of green tea leafstalk which has not been used before but has been discarded can be prepared and applied to the product, it can be used as high value-added functional materials and application products. Therefore, It is worth it.

The green tea leafstarch-derived extract used in the present invention can be prepared by a method known in the art, and the method thereof is not particularly limited, but preferably a method of extracting a water-soluble component contained in green tea leafstalk is used.

Specifically, 5 times of hot water (purified water having a temperature of 80 to 90 ° C) relative to the weight of green tea petiole was added to the dried green tea petiole, and the mixture was heated and stirred for 2 hours while maintaining the temperature. Separate only water-soluble components. Ethanol corresponding to twice the total weight of the filtrate was added with gentle stirring, chlorophyll of the supernatant was removed, and only the precipitate was collected and hot-air dried. It is kept at 30 ~ 40 ℃ during hot air drying to prevent thermal denaturation. In this way, the green tea leaf extract derived from the present invention can be prepared.

The green tea leaf extract derived from such a method has a composition of glycoprotein containing 0.90% of catechin and 0.12% of caffeine, 22.1% of catechin and 4.61% of caffeine, The components are different, and the effects exhibited thereby are also different from each other.

The composition of the present invention may contain an amount of the green tea petiole extract in an amount of 0.001 to 20% by weight based on the total weight of the composition. If the content of the active ingredient is less than 0.001% by weight, the efficacy and efficacy are insufficient. If the content is more than 20% by weight, it is difficult to expect an increase in the effect according to the amount of raw material.

The green tea leaf extract derived from green tea used in the present invention effectively inhibits the acceleration of the hair follicle from the growing stage to the regenerating stage rather than the normal state due to the stress, thereby suppressing stress-induced hair loss (regressor migration) and enhancing the hair growth promoting effect And is safe for the skin and has high stability, which is preferable for industrial use.

The composition for promoting hair growth of the present invention is not particularly limited in its formulation, but may be a cosmetic composition having a formulation such as a hair tonic, a hair nutritional lotion, a scarf treatment, a hair treatment, a hair rinse, a hair shampoo, .

In addition, in the respective formulations, the ingredients other than the above-mentioned essential ingredients can be suitably selected and blended by those skilled in the art depending on the kind of the external preparation, the purpose of use, and the like.

Hereinafter, the present invention will be described in more detail with reference to examples and test examples, but the present invention is not limited to these examples.

[Example 1] Preparation of extract from green tea leafstalk

Purified water having a temperature of 80 to 90 占 폚 was added in an amount of 5 times as much as the weight of the green tea petiole to the dried green tea leafstalk (Miraikan), and the mixture was heated and stirred for 2 hours while maintaining the temperature. After the temperature was cooled to room temperature, Adventec No. 2 was added. 131 (Adventec MFS, Inc., Japan) to separate only water-soluble components. Ethanol corresponding to twice the weight of the total weight of the filtrate was slowly added with stirring, the chlorophyll of the supernatant liquid was removed, and the precipitated solids were collected and subjected to hot air drying while maintaining the temperature at 30 to 40 ° C to extract the green tea leaf extract- 1 to 3% by weight of the green tea leaf sack in the form of a white powder.

[Comparative Example 1] Preparation of green tea leaf extract

Purified water having a temperature of 80 to 90 ° C was added to commercially available green tea leaves (AMOREPACIFIC Co., Ltd.) in an amount of 5 times as much as the weight of green tea leaves, and the mixture was heated and stirred for 2 hours while maintaining the temperature. After the temperature was cooled to room temperature, Adventec No. 2 was added. 131 (Adventec MFS, Inc., Japan) to separate only water-soluble components. Filtered, concentrated using a vacuum distillation apparatus, and dried using a freeze dryer to obtain a green tea leaf extract as a yellowish brown powder.

[Test Example 1] Estimation of hair loss inhibition efficacy

Stress induces the secretion of Substance P from the nerve endings and causes macrophages, γδ T cells and mast cells to pass through the substance P receptor (NK-1 receptor) to the inflammatory signaling substance (cytokine (FASEB J. 2001; 15 (13): 2536-2538). In addition, it has been shown that the production of keratinocytes in hair follicles can be inhibited by stress and by inhibiting the growth of keratinocytes and inducing apoptosis (Am J Pathol. 2003; 162 (3): 709-712) have been proposed to prevent degranulation of a series of mast cells due to antagonists or stress on the substrate P receptor in order to prevent hair loss.

Therefore, in this test, the effect of green tea leafstock-derived extract on the degree of degranulation of mast cells was measured. In order to examine the inhibitory effect on degranulation, which is an indicator of immediate response to allergic reaction, β-hexosaminidase Secretion was measured.

The mast cells (RBL-2H3 cells) were suspended in DMEM containing 10% FBS, and then the cells were divided into 48 well plates (Corning, NY, USA) in an amount of 5 × 10 ⁵ cells / ml. The cells were then sensitized with anti-DNP IgE (30 ng / ml) and cultured in a 37 ° C 5% CO ₂ incubator for 24 hours. Cells of each well were washed twice with Siraganian buffer (119 mM NaCl, 5 mM KCl, 0.4 mM MgCl ₂ , 25 mM PIPES, 40 mM NaOH, pH 7.2), and then 5.6 mM glucose, 1 mM CaCl ₂ The shiraganian buffer containing 0.1% BSA was added and the green tea leaf extract of Example 1 and the green tea leaf extract of Comparative Example 1 were incubated for 1 hour at a concentration of 0, 50, 100 ppm in a 37 ° C 5% CO ₂ incubator , Treated with DNP-HSA (10 μg / ml) for 1 hour, allowed to stand in an ice bath for 10 minutes, and then terminated. 20 μl of the supernatant was transferred to a 96-well plate, and 80 μl of a substrate buffer (1 mM 4-p-nitrophenyl-N-acetyl-bD-glucosaminide, sodium citrate 0.05M, pH 4.5) was added and incubated at 37 ° C for 30 minutes stop solution per well _{(0.1M Na 2 CO 3 / NaHCO} 3, pH 10.0) and the reaction was terminated by the addition of 200μl. Absorbance was measured at 405 nm using a microplate reader (Molecular Devices Co. Ltd., USA). The inhibition rate (%) was calculated by the following equation (1) as the absorbance value of green tea leafstalk extract and green tea leaf extract treated group and control group.

C: cells (+), DNP-HSA (+), test sample (-)

T: cells (+), DNP-HSA (+), test sample (+)

The measurement results are shown in Table 1 below.

Test substance Degranulation degree of mast cell Control group (no addition) 100 Example 1 50 ppm 86 100ppm 81 Comparative Example 1 50 ppm 95 100ppm 93

Table 1 shows that the treatment of Comparative Example 1, which is a green tea leaf extract, inhibited the degranulation of mast cells, but the degree of degranulation was not so high. On the other hand, in Example 1 of the green tea leaf extract, It can be confirmed that degranulation is effectively suppressed.

Thus, it can be seen that the green tea petiole extract used in the present invention blocks the production of intracellular inflammatory substances and thus can effectively prevent hair loss due to stress.

[Test Example 2] Evaluation of skin stability

In order to examine the degree of stimulation of the skin of the green tea petiole extract used in the present invention, a human skin primary stimulation test was conducted.

The study consisted of 34 healthy adult women and 6 men (mean age 35.1 years) in the first round. In the second round, 28 women and 2 men totaled 30 Age 30.1 years) were included in this study.

Specifically, a solution prepared by dissolving the green tea leaf extract obtained in Example 1 in water to a concentration of 10% was applied evenly to the skin of the back region in an amount of 20 mg / cm ² .

After the application, the patch was attached to the area, and after 24 hours, the patch was removed, and the first reading was performed 30 minutes later and the second reading was performed after 24 hours. 1+ was positive and the final reading was considered definitive. To determine the intensity of the skin irritation of the sample, weights were assigned according to the degree of positive skin reaction (-, +, ++, +++, ++++, 0, 1, 2, 3, 4 To give an average response of the skin. Specific criteria are given in Table 2 below. In addition, the calculation of the skin averaged response was calculated by the following equation (2), and the results are shown in Table 3 below.

Criteria reaction weight Criteria for judgment - 0.0 : No reaction + 1.0 : Faint erythema ++ 2.0 : The border is clear but weak erythema, edema and palsy +++ 3.0 : Marked erythema, papule and blister ++++ 4.0 : Algebra

Result criteria Skin mean response Criteria 0.0 to 0.9
1.0 to 2.9
3.0 ~ 4.9
5.0 or higher No stimulation
Light stimulus
Moderate irritation
River stimulation

The results were as follows: Average skin response was 0.0 ~ 0.9 after using green tea leafstalk extract.

Hereinafter, a formulation example of the composition according to the present invention will be described. However, the pharmaceutical composition and the cosmetic composition can be applied to various formulations, which are not intended to limit the present invention but merely to illustrate the present invention.

[Formulation Example 1] Hair tonic

Hair tonics were prepared according to the compositions shown in Table 4 by the conventional method.

Raw material name Content (% by weight) ethanol 50 menthol 0.02 glycerin 3 Salicylic acid 0.05 Green tea leafstalk extract 1.0 Preservatives, fragrances and pigments Suitable amount Purified water Balance

[Formulation Example 2] Hair lotion

Hair lotions were prepared in a conventional manner according to the composition shown in Table 5 below.

Raw material name Content (% by weight) Cetostearyl alcohol 2.0 EDTA 2Na 0.2 Hydroxyethyl cellulose 0.5 Mineral oil 5.0 Green tea leafstalk extract 1.0 Preservatives, fragrances and pigments Suitable amount Purified water Balance

[Formulation Example 3] Hair nutrition lotion

Hair nutritional lotion was prepared according to a conventional method according to the composition shown in Table 6 below.

ingredient Weight ratio (%) Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.7 Mineral oil 10.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Green tea leafstalk extract 1.0 antiseptic Proper amount Spices and Pigments Proper amount Purified water Balance (to 100)

[Formulation Example 4] Hair shampoo

Hair shampoos were prepared in a conventional manner according to the composition shown in Table 7 below.

ingredient weight% Purified water Balance (To 100) Green tea leafstalk extract 1.0 Sodillauryl sulphite 36.0 Cocamidopropyl betaine 8.0 Palmitidine dimalate 2.0 Glycollosteralate 1.5 Polyquaternium 10 0.5 Saitric Acid 0.1 glycerin 2.0 Preservatives, coloring and flavoring Suitable amount

[Formulation Example 5] Hair conditioner

The hair rinse was prepared according to a conventional method according to the composition shown in Table 8 below.

ingredient weight% Purified water Balance (To 100) Green tea leafstalk extract 1.0 Propylene glycol 2.0 Cetyltrimethylammonium chloride 1.0 Cetyl alcohol 3.0 Stearyl alcohol 3.0 Mineral oil 0.5 Saitric Acid 0.2 Polydimethylsiloxane 1.0 Preservatives, coloring and flavoring Suitable amount

Claims (4)

A composition for external application for skin for preventing hair loss or promoting hair growth comprising an extract derived from green tea leafstalk as an active ingredient. The composition for external application for skin according to claim 1, wherein the extract is contained in an amount of 0.001 to 20% by weight based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the green tea leafstalk-derived extract is extracted from green tea leafstalk using water as a solvent. The composition for external application for skin according to claim 3, wherein the water is hot water at 80 to 90 ° C.