KR102174309B1 - Cosmetic composition for prevention of losing hair or promotion of growing hair containing Taxillus yadoriki extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing hair loss or promoting hair growth, containing a Taxillus yadoriki extract and, more specifically, to a cosmetic composition having effects of preventing hair loss or promoting hair growth by promoting the proliferation of dermal papilla cells and the expression of hair growth factors.

Description

Cosmetic composition for prevention of losing hair or promotion of growing hair containing Taxillus yadoriki extract, containing oak mistletoe extract

The present invention relates to a cosmetic composition for hair containing an oak mistletoe extract and showing an effect of preventing hair loss or promoting hair growth.

Human hairs are about 100,000 to 150,000 and are formed in hair follicles. Each hair has a different cycle and grows and falls through the growth phase, degeneration phase, and resting phase. This cycle is repeated over 3 to 6 years, and an average of 50 to 100 hairs per day are normally removed.

Alopecia refers to an abnormal increase in the number of hairs dropping out due to the shortening of the growth period hairs and the increase of degenerative or telogen hairs among these cycles. Alopecia is largely divided into androgenetic alopecia, gynecomastia, and alopecia areata. The causes include abnormality of the hair cycle and weakening of the hair roots due to the action of male hormones, and dermal papilla cells and geminal matrix related to hair cycle regulation. cell) function decline or inhibition of proliferation, abnormal changes in the hair cycle due to decreased blood flow to the scalp, aging, skin diseases, anticancer drugs, mental stress, physical stimulation, nutritional deficiency, the action of pathological factors, and environmental pollution. Hair loss occurs when these causes act alone or in combination.

Treatments known to be effective in preventing hair loss or promoting hair growth until now include the application of a minoxidil solution or the use of trichosaccharide. Minoxidil was developed as a vasodilator for the treatment of hypertension, but it was developed as a hair growth agent as hirsutism was reported as a side effect. The mechanism of action for the hair growth effect of minoxidil has not yet been clearly identified, but it is believed that hair growth is induced by an increase in nutrient supply through vasodilation. In addition, finasteride is a substance that inhibits the activity of 5 alpha-reductase, an enzyme that acts on male hormone metabolism, and was initially developed as a treatment for prostatic hyperplasia, but as a side effect, hirsutism was reported as a hair growth agent.

However, since the effect of minoxidil and finasteride disappears when the use is discontinued, there is a discomfort in that they must be used continuously.In the case of minoxidil, side effects such as sticky feeling and skin irritation are reported, and in the case of finasteride, sexual dysfunction such as dysfunction and erectile dysfunction are reported. It has been reported, and there are restrictions on continuous use.

Republic of Korea Patent Publication No. 10-2019-0026372 (published on March 13, 2019) relates to a cosmetic composition containing natural extracts for preventing hair loss and promoting hair growth, and is a natural extract of the first group including phalanx skin extract and flavor extract At least one selected from among; And, at least one selected from the second group of natural extracts including seokchangpo extract and malt extract; comprising as an active ingredient, a cosmetic composition having a function of preventing hair loss and promoting hair growth is disclosed. Republic of Korea Patent Registration No. 10-1540333 (registered on July 23, 2015) relates to a method for preparing a natural extract with improved anti-hair loss effect and a shampoo for preventing hair loss containing a natural extract prepared by the method, Bonsam, Graviola And there is disclosed a method for producing a natural extract with improved anti-hair loss effect, characterized in that prepared by extracting after adding water to a mixture of Moringa.

The present invention is to provide a natural product that exhibits an effect of preventing hair loss or promoting hair growth without side effects such as feeling of use, skin irritation, and sexual dysfunction.

The present invention provides a cosmetic composition for preventing hair loss or promoting hair growth, comprising an oak mistletoe extract.

In the present invention, the oak mistletoe extract is preferably an ethanol extract, more preferably an ethyl acetate fraction of an ethanol extract or an n -butanol fraction of an ethanol extract.

In the present invention, the oak mistletoe extract is preferably contained in an amount of 0.1 to 30% by weight based on the total composition.

The present invention provides a cosmetic composition for preventing hair loss or promoting hair growth derived from natural products without side effects such as feeling of use, skin irritation, and sexual dysfunction.

The oak mistletoe extract of the present invention proliferates dermal papilla cells and promotes the expression of hair growth factors, thereby exhibiting excellent hair loss prevention or hair growth promoting effects.

1 is a view showing the effect of proliferating dermal papilla cells of camellia mistletoe extract, chestnut mistletoe extract, and oak mistletoe extract.
Figure 2 is a schematic view showing a process of preparing a fraction from the oak mistletoe extract.
Figure 3 is a view showing the dermal papilla cell proliferation effect of fractions of oak mistletoe extract fractionated with n -hexane (Hex), methylene chloride (MC), ethyl acetate (EA), n -butanol (BuOH) and water (DW) .
Figure 4 is a graph showing the expression rate of β-catenin for the oak mistletoe extract, the oak mistletoe ethyl acetate (EA) fraction, and the oak mistletoe n -butanol (BuOH) fraction.
Figure 5 is a graph showing the expression rate of hair growth factors for oak mistletoe extract, oak mistletoe ethyl acetate (EA) fraction, oak mistletoe n -butanol (BuOH) fraction.

The present invention provides a cosmetic composition for preventing hair loss or promoting hair growth, comprising an oak mistletoe extract.

Mistletoe is an evergreen parasitic shrub of the family Mistletoe family, and it is said to be a semi-parasitic plant because it parasitizes on oak, pear, chestnut, hackberry, and elm trees. The present invention is to provide a cosmetic composition for preventing hair loss or promoting hair growth using oak mistletoe, which has the best proliferation effect of dermal papilla cells among mistletoes, camellia mistletoe, chestnut mistletoe, and oak mistletoe.

Hair grows and falls through the growth phase, degeneration phase, and telogen phase, and this cycle repeats over 3-6 years. Hair loss is a symptom of an abnormal increase in the number of hairs falling out due to a shortening of the proportion of hairs in the growth phase and more hairs in the degenerative or telogen phase. The causes of hair loss are very diverse, and hair loss is typically caused by male hormones.

As the male hormone dihydrotestosterone activates the hair loss inducer DKK-1, the Wnt signaling substance (Wnt/β-catenin) decreases, resulting in hair loss. For this reason, finasteride is used to treat hair loss by reducing dihydrotestosterone, but this is a problem because it has side effects that cause sexual dysfunction such as dysfunction and erectile dysfunction.

Accordingly, the present invention was to develop a composition derived from natural products having an effect of preventing hair loss or promoting hair growth, and ensuring safety and efficacy when used, and it was confirmed through an experiment that the oak mistletoe extract increases the expression of β-catenin.

β-catenin is a signaling protein that plays an important role in the formation of hair follicles. When the Wnt/β-catenin pathway is activated and the signal of β-catenin increases, the stem cells produce hair through the hair cells, but when the signal of β-catenin decreases, the stem cells produce skin cells. do. Therefore, it was confirmed that the oak mistletoe extract of the present invention promotes hair generation as it increases the expression of β-catenin.

In addition, IGF-1 and VEGF are hair-related growth factors, IGF-1 induces the transition from the resting state of the hair to the growing state, and VEGF promotes hair growth by regenerating blood vessels in the dermal papilla. The present invention confirmed that the oak mistletoe extract increases the expression of IGF-1 (insulin-like growth factor) and VEGF (vascular endothelial growth factor).

On the other hand, in the oak mistletoe extract of the present invention, the extract may be prepared according to a conventional method known in the art, that is, using a conventional solvent under conditions of a normal temperature and pressure, for example, water, carbon number An extraction solvent selected from the group consisting of 1-4 anhydrous or water-containing lower alcohol, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane, and mixtures thereof It can be obtained through any one of an extraction method selected from a solvent extraction method, a supercritical extraction method, and an ultrasonic extraction method used.

The oak mistletoe extract of the present invention is preferably an ethanol extract, more preferably an ethyl acetate fraction of the ethanol extract or an n -butanol fraction of the ethanol extract.

In addition, the oak mistletoe extract is preferably contained in an amount of 0.1 to 30% by weight based on the total weight of the cosmetic composition. When the content of the extract is less than 0.1% by weight, the effect of preventing hair loss or promoting hair growth is insignificant, and when it exceeds 30.0% by weight, even if the content of the oak mistletoe extract in the cosmetic composition increases, hair loss that can be expected at a certain concentration or higher Alternatively, the hair growth promotion effect may no longer be enhanced, and it is not appropriate because the formulation stability cannot be maintained.

On the other hand, the cosmetic composition of the present invention may be any one selected from hair serum, hair tonic, hair nourishing lotion, hair treatment, hair shampoo, hair rinse, hair lotion, hair gel, and scalp hair combination treatment. However, this may be commonly used in the field of cosmetic products for scalp and hair, and is not limited to the above formulation, and may be appropriately selected and blended by a person skilled in the art according to the type or purpose of use of other external preparations.

In addition, the cosmetic composition of the present invention may contain auxiliary agents commonly used in the cosmetic field, such as hydrophilic or lipophilic activators, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, odorants, dyes, and the like. The amounts of these various adjuvants are those commonly used in the art, such as 0.001 to 30% by weight based on the total weight of the composition. However, in any case, the adjuvant and its ratio will be selected so as not to adversely affect the desirable properties of the cosmetic composition according to the present invention.

In addition, the cosmetic composition of the present invention can be used in conjunction with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use may be varied according to a user's skin condition or taste.

Hereinafter, the present invention will be described in more detail in the following Examples and Experimental Examples. However, the scope of the present invention is not limited to the following examples and experimental examples, and includes all modifications of the equivalent technical idea.

[Example 1: Preparation of camellia mistletoe, chestnut mistletoe, oak mistletoe extract]

The branches of camellia mistletoe, chestnut mistletoe, and oak mistletoe were pulverized after shading, respectively, to prepare a crushed mistletoe. After mixing 50 g of each mistletoe crushed product and 500 g of 70% (v/v) ethanol, heating it at 80° C. for 3 hours in an extractor equipped with a cooling condenser, filtering it with 400 mesh, and then twice Whatman Filtered through filter paper. Each extract was concentrated under reduced pressure at a temperature of 55° C. with a distillation apparatus equipped with a cooling condenser, and the residue was repeated twice under the same conditions. Camellia mistletoe extract 12.1 g, chestnut mistletoe extract 13.8 g, oak mistletoe extract 12.6 g were obtained.

[Experimental Example 1: Evaluation of cell proliferation on dermal papilla cells of each mistletoe extract]

In this experimental example, the cell proliferation effect of the extract of camellia mistletoe, chestnut mistletoe, and oak mistletoe prepared in Example 1 was to be confirmed on dermal papilla cells.

Human follicle dermal papilla cells (HFDPC) were dispensed into a 96-well plate at 2×10 ⁴ cells/ml, stabilized for 24 hours in a 37°C, 5% CO ₂ incubator, and treated by concentration to 48 It was allowed to react for time. Thereafter, 20 μl of MTT reagent at a concentration of 5 mg/ml was added to each well, and reacted in an incubator at 37° C. and 5% CO ₂ for 2-3 hours. All the supernatant of each well after the reaction was removed, and 100 µl of dimethyl sulfoxide (DMSO) was added to completely lyse the cells, and the absorbance was measured at 550 nm. The results are shown in FIG. 1.

As shown in FIG. 1, the camellia mistletoe extract and the chestnut mistletoe extract showed poor cell proliferation of about 107%, whereas the oak mistletoe extract showed about 120% of dermal papilla cell proliferation.

[Example 2: Preparation of oak mistletoe fraction]

The oak mistletoe extract obtained in Example 1 was suspended in purified water and fractionated sequentially according to polarity using a fractionation funnel. A total of 5 solvent fractions were obtained in the order of n -hexane (Hex), methylene chloride (MC), ethyl acetate (EA), n -butanol (BuOH) and water (DW). Each of the obtained fractions was concentrated under reduced pressure to finally prepare an oak mistletoe fraction. The amount of the prepared fractions are shown in Table 1 below, and the steps of obtaining the fractions are briefly shown in FIG. 2.

Fraction Dry weight of the final fraction (g) n -hexane (Hex) 3.8 Methylene chloride (MC) 4.1 Ethyl acetate (EA) 7.7 n -butanol (BuOH) 30.7 Water(DW) 51.0

[Experimental Example 2: Evaluation of cell proliferation on dermal papilla cells of oak mistletoe fraction]

In this experimental example, the effect of cell proliferation on dermal papilla cells of the oak mistletoe fraction obtained in Example 2 was examined.

Human follicle dermal papilla cells (HFDPC) were dispensed into a 96-well plate at 2×10 ⁴ cells/ml, stabilized for 24 hours in a 37°C, 5% CO ₂ incubator, and treated by concentration to 48 It was allowed to react for time. Thereafter, 20 μl of MTT reagent at a concentration of 5 mg/ml was added to each well, and reacted in an incubator at 37° C. and 5% CO ₂ for 2-3 hours. All the supernatant of each well after the reaction was removed, and 100 µl of dimethyl sulfoxide (DMSO) was added to completely dissolve the cells, and the absorbance was measured at 550 nm. The results are shown in FIG. 3.

As shown in FIG. 3, it was confirmed that the ethyl acetate (EA) fraction of oak mistletoe and the n -butanol (BuOH) fraction of oak mistletoe exert a cell proliferative effect on dermal papilla cells.

[Experimental Example 3: Wnt/β-catenin expression rate confirmation]

In this experimental example, the Wnt/β-catenin expression rate of the oak mistletoe extract prepared in Example 1, the oak mistletoe ethyl acetate (EA) fraction and n -butanol (BuOH) fraction prepared in Example 2 were to be confirmed.

Human follicle dermal papilla cells (HFDPC) were dispensed into 6 wells at a concentration of 5×10 ⁵ cells/ml, and cultured in an incubator at 37° C. and 5% CO ₂ for 24 hours. The cultured cells were collected and lysed, and the protein was quantified using BCA assay (bicinchoninic acid assay). The quantified protein was electrophoresed on a 10% SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) gel, and then transferred to a poly-vinyl difluoride (PVDF) membrane.

After the primary antibody (β-catenin, santa cruz, USA) was shaken overnight at 4° C., the secondary antibody to which HRP (horseradish peroxidase) was bound was reacted at room temperature for 2 hours. Then, it was washed 3 times with 1X TBST, and the band was confirmed using an electro-chemiluminescence (ECL) reaction kit. The intensity of each band was quantified based on the expression of β-actin, and the expression rate of β-catenin was calculated.

As shown in Figure 4, it was confirmed that both the oak mistletoe extract, the oak mistletoe ethyl acetate (EA) fraction, and the oak mistletoe n -butanol (BuOH) fraction increased β-catenin expression, and in particular, excellent expression rate in the ethyl acetate (EA) fraction Was confirmed.

[Experimental Example 4: Confirmation of hair growth factor expression promoting effect]

In this experimental example, the hair growth factor expression rate of the oak mistletoe extract prepared in Example 1, the oak mistletoe ethyl acetate (EA) fraction and the n -butanol (BuOH) fraction prepared in Example 2 was to be confirmed.

Human follicle dermal papilla cells (HFDPC) were dispensed into 96 wells at a concentration of 1×10 ⁵ cells/ml, and cultured in an incubator at 37° C., 5% CO ₂ for 24 hours, and then prepared in Example 1. The oak mistletoe extract and the oak mistletoe ethyl acetate (EA) fraction prepared in Example 2, and the oak mistletoe n -butanol (BuOH) fraction were treated by concentration (% (v/v)) at 37° C., 5% CO ₂ incubator Reacted for 24 hours. It was measured by enzyme-linked immunosorbent assay (ELISA) using the culture medium of the cells.

The expression of IGF-1 (Insulin-like growth factor) and VEGF (Vascular endothelial growth factor) in the cell culture was analyzed using an ELISA kit, and absorbance was taken at 450 nm and the amount of each produced was shown in FIG. 5.

As shown in FIG. 5, it was confirmed that both the oak mistletoe extract, the oak mistletoe ethyl acetate (EA) fraction, and the oak mistletoe n -butanol (BuOH) fraction increased the expression of hair growth factors, and in particular, the oak mistletoe ethyl acetate (EA) fraction It was confirmed the excellent expression rate of the hair growth factor in.

[Production Example 1: Shampoo Containing Oak Mistletoe Extract]

Table 2 shows an example of preparing a shampoo containing the oak mistletoe extract of the present invention.

ingredient Manufacturing Example 1
Content (% by weight) Comparative Example 1
Content (% by weight) Oak mistletoe extract of Example 1 5.0 - Oak mistletoe of Example 2
Ethyl acetate (EA) fraction 5.0 - Oak mistletoe of Example 2
n -butanol (BuOH) fraction 5.0 - Disodium EDTA 0.03 0.03 Polyquaternium-10 0.1 0.1 Ammonium lauryl sulfate 40.5 40.5 Ammonium laureth sulfate 15.5 15.5 Cocamidopropylbetaine 6.0 6.0 Propylene glycol 2.0 2.0 Sodium xylenesulfonate 2.0 2.0 Glycol distearate 3.0 3.0 Cocamide D.A. 1.0 1.0 Lauramide D.A. 1.0 1.0 Salicylic acid 0.13 0.13 Simethicone 3.0 3.0 antiseptic Appropriate amount Appropriate amount incense Appropriate amount Appropriate amount Purified water Balance Balance system 100.0 100.0

[Preparation Example 2: Rinse Containing Oak Mistletoe Extract]

Table 3 shows an example of the preparation of a rinse containing the oak mistletoe extract of the present invention.

ingredient Manufacturing Example 2
Content (% by weight) Comparative Example 2
Content (% by weight) Oak mistletoe extract of Example 1 5.0 - Oak mistletoe of Example 2
Ethyl acetate (EA) fraction 5.0 - Oak mistletoe of Example 2
n -butanol (BuOH) fraction 5.0 - Disodium EDTA 0.02 0.02 Hydroxyethyl cellulose 0.2 0.2 Panthenol 0.05 0.05 Propylene glycol 1.5 1.5 glycerin 2.0 2.0 Cetyl alcohol 3.5 3.5 Stearyl alcohol 1.5 1.5 Glyceryl Stearate SE 0.5 0.5 Dimethicone 1.5 1.5 antiseptic Appropriate amount Appropriate amount incense Appropriate amount Appropriate amount Purified water Balance Balance system 100.0 100.0

[Production Example 3: Hair Tonic Containing Oak Mistletoe Extract]

An example of preparing a hair tonic containing the oak mistletoe extract of the present invention is shown in Table 4 below.

ingredient Manufacturing Example 3
Content (% by weight) Comparative Example 3
Content (% by weight) Oak mistletoe extract of Example 1 5.0 - Oak mistletoe of Example 2
n -hexane (Hex) fraction 5.0 - Oak mistletoe of Example 2
Methylene chloride (MC) fraction 5.0 - Disodium EDTA 0.02 0.02 Panthenol 0.5 0.5 Butylene Glycol 10.0 10.0 ethanol 18.0 18.0 menthol 0.03 0.03 Polysorbate 80 1.0 1.0 Imidazolidinyl urea 0.2 0.2 Biotin 0.1 0.1 incense Appropriate amount Appropriate amount Purified water Balance Balance system 100.0 100.0

Claims (4)

Contains oak mistletoe ( Taxillus yadoriki ) extract,
The oak mistletoe extract is an ethyl acetate fraction of ethanol extract or n -butanol fraction of ethanol extract, and expresses beta-catenin, insulin-like growth factor (IGF-1), and vascular endothelial growth factor (VEGF). Cosmetic composition for preventing hair loss or promoting hair growth, characterized in that to increase.
delete delete The method of claim 1,
The oak mistletoe extract,
A cosmetic composition for preventing hair loss or promoting hair growth, characterized in that it contains 0.1 to 30% by weight based on the total composition.

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