KR101827655B1 - A composition comprising banana having inhibitory effect on induction of grey hair and positive effect on induction of black hair - Google Patents

Abstract

The present invention relates to a composition for preventing white hair and promoting induction of black hair. The composition comprising bananas for preventing white hair and promoting induction of black hair according to the present invention may contain a banana extract. By using a natural composition showing the scavenging ability and the reducing power against the active oxygen for H_2O_2 DPPH radical according to the composition for preventing white hair and promoting induction of black hair, white hair can be prevented and black hair production can be promoted. Particularly, for the composition for preventing white hair and promoting induction of the black hair, an extraction composition derived from natural materials is provided as a composite composition to have a significant effect on prevention of white hair and promotion of black hair induction without side effects of skin or hair damage.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for prevention of gray hair including bananas and a composition for accelerating gray scale induction,

The present invention relates to a composition for prevention of gray hair and a composition for promoting black hair induction comprising a banana. More specifically, by using a natural composition showing the ability to scavenge H ₂ O ₂ DPPH radicals and reduce the reactive oxygen species, H ₂ O ₂ accumulated around the hair follicle is reduced, melamine synthesis is promoted, And to provide a composition for preventing gray hair and for promoting the induction of black hair, which can induce black hair.

The hair consists largely of three parts: skin, fur, and water quality. The follicle surrounding the water quality is the thickest part (80-90%) of the hair, melanocyte melanin pigment and lipid membrane and TRP-1 (Tyrosinase Related Protein-1), TRP-2 (Tyrosinase Related Protein-2) It contains granules composed of other proteins. The melanin pigment is synthesized in the melanocyte, the organelle in the melanocyte, and is transferred to the surrounding keratinocyte through the resin dendrites of the melanocyte. In the synthesis pathway of melanin, tyrosinase is involved first at the stage where the amino acid tyrosine is oxidized and the waveguide is re-oxidized to generate dopaquinone, and the subsequent reaction proceeds auto-oxidatively. However, TRP-1 and TRP-2 Are involved and are known to accelerate the reaction. The color of the hair is determined by the factors such as the degree of the type and distribution of the eumelanin and peomelanin, which are formed from the amino acid tyrosine, and reveals the hair color unique to each person. In other words, melanin type and size of the granules according to the size of the hair, such as black, red, blonde and can be determined. The color of the hair is the two most common melanin color toners in the middle layer of the hair (cortex). Eumelanin is the most common and darkest pigment, and it produces brown and black hair color. Peo melanin is a lighter hue with blonde hair. The color of red hair is shown by red color and black color, and blond hair is the result of mixing red color and yellow color. Sandy brown hair is pigmented by a mixture of red, brown and black pigments, and dark brown hair contains more black pigment than sandy brown hair. And there is no coloring matter in the gray hair. Hair color is determined by the pigment present in the hair, and this pigment is determined by the genetic information factor of the person.

Melanin is a water-insoluble protein that imparts color to hair. Tyrosine, the amino acid produced in the dermal papilla, is a substrate for melanin. Because white hair stops its action in dopaquinone, hair is formed by keratinization and ascending above keratinization, and it is a white hair which has not been oxidized because it can not make enzyme. White moths are particularly evident in races with dark brown hair, and are a type of aging phenomenon that occurs because melanogenesis stops in melanocytes.

Dopaquinone reacts in two ways. There is a pathway for producing dark brown colored eumelanin via dopachrome and a pathway for producing red brown pigmented melanin after dopaquinone is bound to cysteine present in keratin protein. Thus, the oxidative dyeing reaction of hair is the result of the polymerization reaction in the hair. In this reaction, aromatic amines and phenol monomers oxidize and polymerize and also bind to the amino acid residues in the hair. While eumelanin is relatively large and chemically easily destroyed, peomelanin is relatively small in size and chemically stable. The hair with high cysteine content has a lot of peomelanin, and when dark brown hair is decolorized with hydrogen peroxide, it appears that the color is slightly red because the melanin is first destroyed and the peomelainin remains.

Melanin in hair is an important factor that determines the hue of hair. It is transferred to hair cortical cell keratinocytes generated in the melanocyte present in the hair, and then moves upward with hair growth. The gray hair, recognized as a phenomenon of physiological aging, is caused by a decrease in the number of hair melanocytes and a decrease in the production of melanin due to a decrease in function of melanocytes.

The gray hair generally occurs when the age is higher, the impression of old age deepens the impression, and especially the gray hair in young age generally dislikes. For this reason, in order to hide gray hair, many dyeing agents for dyeing gray hair out by white hair dyeing or the like have been studied, and they have been widely marketed. However, black hair dyeing has to be dyed again by the growth of gray hair in a temporary manner, and oxidized black hair dyeing, which is now widely used, causes skin damage by the use of an oxidizing agent or the like. Therefore, various studies have been made on a black mold inhibitor that prevents the occurrence of white feathers or a black feather promoter that induces the production of black feathers.

However, when a compound is used, side effects such as damaging hair or skin are problematic, and there is a problem that the use amount thereof is limited due to cytotoxicity and the like. In the case of natural products, a composition derived from natural products, The effect is relatively small.

Accordingly, a composition for prevention of gray hair and a composition for promoting hair growth, which can prevent black hair and promote black hair generation more effectively while solving the problems of side effects such as hair or skin damage due to use of a composition by using a composition derived from a wider variety of natural ingredients The development and the discovery of such materials are urgently required.

In related art prior art, KR 10-2002-0059906 A (prior art 1, public date: July 16, 2002) was formulated with black sesame seeds, And a method of using the same. In addition, KR 10-2002-0059905 A (prior art 2, public date: July 16, 2002) is used in a container with red algae, cedar leaf, jellyfish, (Japanese Unexamined Patent Publication No. 56-087515 A (prior art 3, published on July 16, 1981) discloses a method of keeping black beans at a temperature of 50 ° C, , And a method using kelp. Japanese Patent Publication No. JP 60-178805 A (prior art 4, publication date: September 12, 1985) discloses a method of using plant extracts using an extract of Ocarki as an active ingredient. In addition, a method of using pituitary hormone (Japanese Patent Publication No. 62-63510), C-AMP (adenosine-3,5'-cyclic phosphate) (Japanese Patent Publication No. 62-45527) (Japanese Examined Publication No. 60-174705).

KR 10-2002-0059906 A KR 10-2002-0059905 A JP 56-087515 A JP 60-178805 A

The object of the present invention is to provide a method for preventing gray fever and prevention of gray fever including banana for preventing white fever and promoting the production of black blood using a natural composition showing the ability of scavenging H ₂ O ₂ DPPH radical and reducing the active oxygen, And to provide a composition for promotion.

In particular, it is intended to provide a composite composition which is capable of exhibiting a significant effect on prevention of gray hair and promotion of black cohosh without inducing side effects of skin or hair damage by utilizing an extract composition derived from natural materials.

Another object of the present invention is to provide a composition for prevention of gray hair and a composition for promoting hair growth, comprising banana which can be used as an external preparation for hair through excellent flavor while promoting gray hair prevention including banana by providing a certain range of banana extract composition And to provide a hair external preparation for prevention of gray hair and a hair tonic for promoting hair growth.

It is still another object of the present invention to provide a method for the prevention of gray hair and a composition for promoting gray hair induction, which comprises the banana.

In order to accomplish the above object, the present invention provides a composition for promoting the prevention of gray hair and for promoting hair growth, which comprises a banana extract.

The composition for preventing gray hair and for promoting gray hair induction may further comprise a black sugar extract and a red mud safflower extract.

The composition for preventing gray hair and promoting gray hair induction may further comprise a black rice extract and a brown rice extract.

The composition for preventing the gray hair and promoting the induction of the black hair may further comprise a black sugar extract, a red mulberry extract, a black rice extract, a brown rice extract and a western blotting extract.

The composition for promoting the prevention of gray hair and the composition for promoting hair growth may be one obtained by extracting 10 to 99% by weight of ethanol with a solvent in the banana extract, the black sugar extract, the red moss extract, the black rice extract, the brown rice extract and the western blotting extract.

In another embodiment of the present invention, the food composition for promoting the prevention of black hair and the composition for promoting black cohosh may include the composition for promoting the prevention of gray hair and the composition for promoting black cohosh.

The composition for preventing hair gray and for promoting hair growth according to another embodiment of the present invention may include the composition for preventing gray hair and promoting hair growth.

In another embodiment of the present invention, the hair cleansing composition for preventing gray hair and promoting hair growth may include the composition for preventing hair graying and promoting hair growth.

A method for preparing a composition for preventing and inhibiting the development of gray fever according to another embodiment of the present invention comprises drying and pulverizing a banana, adding 10 to 99% by weight of an ethanol solvent and the banana pulp in a ratio of 10: 1 to 1: 1 part by weight of the extract to prepare a banana extract; Preparing a black sugar extract by mixing the ethanol solvent of 10 to 99% by weight with the powder of the black starch at a ratio of 10: 1 to 1: 1 by weight after drying and pulverizing the black sugar; A step of preparing an aqueous extract of sodium hypochlorite by mixing and drying 10 to 99% by weight of an ethanol solvent and the above-mentioned aqueous sodium hydroxide solution at a ratio of 10: 1 to 1: 1 by weight; Preparing a black rice extract by mixing and drying the black rice with 10 to 99% by weight of ethanol solvent and the black rice powder at a ratio of 10: 1 to 1: 1 by weight; Preparing a brown rice extract by mixing and drying the brown rice with 10 to 99% by weight of an ethanol solvent and the brown rice powder at a ratio of 10: 1 to 1: 1 by weight; Preparing an extract of Seomyeok-myeon, which is prepared by drying and crushing Seomyo-myeon, mixing 10 to 99% by weight of an ethanol solvent with the above-mentioned Seomye-tai pulverized product at a ratio of 10: 1 to 1: 1; In addition, the method may include a step of mixing the banana extract, the black mangrove extract, the red manganese extract, the black rice extract, the brown rice extract and the western blotting extract.

Hereinafter, the present invention will be described in more detail.

The composition for promoting induction according to one embodiment of the present invention includes a banana extract.

The bananas are grown in wet, low-lying areas in the tropical and subtropical regions, and are one of the oldest representative wild fruits on earth along with human history. Bananas are rich in nutrients such as calcium, carotene, and vitamin A, and are high in calories.

Banana is made up of the husks and the inner pulp, but in general, it is common for bavana to be used to refer to the flesh used for food. However, 'banana' used throughout the specification of the present invention is not limited to banana flesh, but is defined to include both banana flesh and its shell. Therefore, banana extract is defined as belonging to the upper concept of banana peel extract and banana peel extract.

Banana extract promotes melanocyte synthesis as well as cell activity of melanocyte, thereby preventing white moth and promoting the onset of black fever.

Preferably, the banana extract may be a banana peel extract.

To return white hair back to black hair, melanocytes in hair follicles should produce melanin pigment. In addition, melanocytes in the basal layer of the skin synthesize melanin before the defense to protect the skin from harmful stimuli such as ultraviolet light.

In the melanin synthesis mechanism, tyrosinase enzyme is an important catalytic enzyme to determine the initial rate of melanin synthesis reaction. To synthesize melanin, tyrosinase binds a hydroxyl group to tyrosine to produce 3,4-dihydroxyphenylalanine (DOPA) and simultaneously converts DOPA to dopaquinone. The next step is the conversion of dopaquinone to 5,6-Dihydroxy-2-indolycarboxylic acid (DHICA) by the second enzyme tyrosinase related protein-2 (TRP-2). This DHICA is converted to indole-5,6-quinone caboxylic acid by the third enzyme tyrosinase related protein-1 (TRP-1), which is oxidized to produce eumelanin

Melanin is synthesized by oxidation of unstable reactive oxygen species (ROS) such as hydrogen peroxide, superoxide ion and hydroxyl radical. Therefore, when melanin production is promoted by melanocytes in hair follicle cells, white moth can be converted to black hair. Recently, α-melanocyte stimulating hormone (α-MSH), imatinib mesylate and 3-isobutyl-1-methylxanthine (IBMX) have been known to increase melanin production. α-MSH amplifies cAMP, an important messenger for skin pigmentation regulation, and activates protein kinase A (PKA). protein kinase A (PKA) is activated. Thus, tyrosinase activity is regulated to affect melanin cell proliferation and pigmentation.

Imatinib mesylate is a tyrosine kinase inhibitor known as a treatment for chronic myelogenous leukemia (CML) induced by tyrosine kinase. Imatinib mesylate also inhibits c-kit tyrosinase kinase and is involved in the survival and development of c-kit and its ligand stem cell factor melanin cells. IBMX activates PKA by increasing the intracellular cAMP content by inhibiting cAMP phosphodiesterase. PKA phosphorylates the transcription factor CREB and upregulates the major enzymes tyrosinase and MITF, which promote melanin production.

In addition, H ₂ O ₂ accumulates around the hair follicle as a main cause of the change of black hair to white hair, resulting in lowered melanin synthesis and white hair. Therefore, since the composition for preventing gray hair and the composition for promoting gray hair induction has a scavenging ability against H ₂ O ₂ DPPH radical and a reducing power against active oxygen, when the composition for preventing gray hair and using the composition for promoting gray hair induction is used, H ₂ O ₂ to promote the synthesis of melanin to prevent gray hair and promote black hair production.

The extracts of banana peel extracts showed significantly higher effects on black hair than those of banana peel extracts. This banana skin contains tyrosine, tryptophan, iron, potassium, B vitamins, vitamin C and K, magnesium, fiber, antioxidants and copper, which are precursors of melanin synthesis. When oxidized, the tyrosine contained in the banana turns into melanin , And thus it is presumed that the banana bark extract plays a role of enhancing the effect of promoting the black fever induction.

In addition, the banana extract has excellent effect of preventing gray hair and promoting the induction of black cohosh, and has low cell longevity, so that there are few problems such as side effects. In addition, it has an advantage that it can be utilized as a functional product which exhibits a whitish defense function and a black feather stimulating effect by providing a composition containing an extract of banana with various flavors because of its excellent flavor.

That is, the composition for preventing and inhibiting gray hair including the banana is provided with shampoo, rinse, hair gel, ointment, cream, lotion, lotion, spray, ampoule, etc., And can be usefully used as a hair cosmetic for preventing aging of hair.

The composition for preventing gray hair and for promoting gray hair induction may further comprise a black sugar extract and a red mud safflower extract.

The black sesame is a term from black sesame seeds.

The above-mentioned red mulberry is dried root of the red mullet Polygonum multiflorum Thunb. Red mock plants are planted in various places. In autumn or spring, root the roots of the root, grind it, wash it in water, dry it in the sun or steam it and dry it. The taste is sweet and spicy, and the quality is slightly warm. Liver (肝 经), the nerves (肾经) is working. Strengthen liver, kidneys, supplement blood, strengthen bones and muscles. It also makes the stool go through and heal well. In pharmacological experiments, it has been known that intestinal action, intestinal motility enhancement action, cholesterol absorption inhibition in the intestine, and antimicrobial action are known.

When the banana extract further contains the black seed extract and the low water extract, the effect of accelerating the black currant induction is enhanced, so that it is possible to obtain a high effect with a smaller usage amount. Therefore, it is advantageous to keep the cytotoxicity low because it can use less dose based on the effect.

Preferably, the composition for preventing and inhibiting gray hair including the banana comprises a banana extract, wherein 10 to 1000 parts by weight of the blackberry extract and 10 to 1000 parts by weight of the extract are applied to 100 parts by weight of the banana extract By the above range, the effect of promoting the induction of the blotchesis by the mixed use can be improved.

More preferably, the composition for preventing and inhibiting gray hair including the banana comprises a banana extract, wherein 200 to 400 parts by weight of the blackberry extract and 50 to 130 parts by weight of the extract are added to 100 parts by weight of the banana extract, According to the above range, the effect of promoting the induction of black cohosh due to the mixed use of the black sugar extract and the red algae extract is significantly increased, and the cytotoxicity is further lowered. Therefore, the composition can be used as a composition for prevention of gray hair and promotion of black cohosh without any side effects .

The composition for preventing gray hair including the banana and the composition for promoting the induction of the black hair may further comprise a black rice extract and a brown rice extract.

The black rice refers to black rice.

The brown rice refers to rice in which harvested rice is dried and thawed, and then rice husks are removed by a machine made of a rubber roller.

When the above-mentioned black rice extract and brown rice extract are further added to the banana extract, not only the ability to reduce H ₂ O ₂ DPPH radical and the reduction of reactive oxygen species, but also the melanin production can be promoted more effectively .

Preferably, the composition for preventing and inhibiting gray hair including the banana comprises a banana extract, wherein 10 to 1000 parts by weight of the black rice extract and 10 to 1000 parts by weight of the brown rice extract are contained relative to 100 parts by weight of the banana extract , The effect of promoting the induction of the blotchesis by the mixed use can be improved by the above range.

More preferably, the composition for preventing and inhibiting gray hair including the banana comprises a banana extract, wherein 50 to 200 parts by weight of the black rice extract and 50 to 200 parts by weight of the brown rice extract are contained relative to 100 parts by weight of the banana extract have,

By the above range, the scavenging ability for H ₂ O ₂ DPPH radical and the reducing power for active oxygen are greatly improved, and cytotoxicity is lowered, so that even when used in a small amount, it can exert a high effect in promoting the onset of black blood cell.

The composition for preventing and inhibiting gray hair including the banana may further comprise a black sugar extract, a red mulberry extract, a black rice extract, a brown rice extract and a Seomyeonpo extract.

The term " rice bran, " It is a perennial herbaceous plant of beans and its stems and leaves are brown. A yellow flower blooms in July, and a black and round fruit about 5 to 7 mm in diameter is harvested in an oval-shaped pod. According to the Bonchu Gangmok, it is better to use a light soy sauce and a small bean curd while the light is black. In addition, it is said that when sulfur is sprayed and cultivated, the weakness is better, and the nature is warm, the taste is sweet, and there is no poison.

When mixed with banana extract, black mulberry extract, red mulberry extract, black rice extract, brown rice extract, and western blotting extract, the effective ingredient for promoting the expression of TRP-2, an enzyme involved in melanin production, increases, and H ₂ O ₂ DPPH radical is increased, so that it is possible to achieve a higher effect of producing a black blood cell.

Preferably, the composition for preventing and inhibiting gray hair including the banana comprises a banana extract, wherein 200 to 400 parts by weight of a black sugar extract is added to 100 parts by weight of the banana extract, and 50 to 130 parts by weight of the above-mentioned sucrose extract; 50 to 200 parts by weight of the black rice extract; 50 to 200 parts by weight of the brown rice extract and 30 to 150 parts by weight of the extract.

When mixed in the above range, the scavenging ability to H ₂ O ₂ DPPH radical is greatly increased, and the melanin synthesis is promoted, and the effect of inducing the black hair can be remarkably increased.

More preferably, the composition for preventing and inhibiting gray hair including the banana comprises a banana extract, wherein 250 to 350 parts by weight of a black sugar extract and 80 to 110 parts by weight ; 90 to 150 parts by weight of the black rice extract; 90 to 150 parts by weight of the brown rice extract, and 50 to 100 parts by weight of the extract.

By the above range, it is possible to effectively remove H ₂ O ₂ accumulated in the hair follicle by the elimination of H ₂ O ₂ DPPH radicals and the high reducing power against active corals, as well as an enzyme involved in the process of producing melanin The effect of promoting TRP-2 expression and inducing melanin synthesis is most excellent.

In the composition for preventing gray hair, the banana extract, the black manganese extract, the red manganese extract, the black rice extract, the brown rice extract and the western blotting extract may be those obtained by extracting 10 to 99% by weight of ethanol with a solvent.

When the ethanol content of the solvent is less than 10% by weight, an effective ingredient for promoting melanin production is scarcely obtained. When the ethanol content exceeds 99% by weight, a highly cytotoxic ingredient is extracted from the extracted ingredient, And there is a problem that the effect of melanin synthesis is inhibited by extracting components other than the active ingredient.

Preferably, the solvent may be an ethanol extract of 75 to 95% by weight. When ethanol of the above range is used as a solvent, it may be advantageous to obtain an effective ingredient capable of promoting melanin production and effectively removing H ₂ O ₂ accumulated in hair follicles.

In another embodiment of the present invention, the food composition for preventing gray hair and promoting the induction of black hair may include the composition for promoting the prevention of gray hair and the composition for promoting black hair induction.

The food composition according to the present invention is defined as meaning that food, beverage or other food can be ingested due to its functionality or palatability.

Preferably, the food composition for preventing and inhibiting gray fever comprising the banana comprises 11 to 20 parts by weight of a cherry extract, 8 to 20 parts by weight of a dandelion extract, , And 1 to 5 parts by weight of Crassulaceae extract.

When the above-mentioned cherry extract, gondolb extract and mulberry extract are included in the above-mentioned range, the flavor of the banana extract can be well balanced with the sweet and sour aroma of the banana extract as a whole, and a deep flavor can be obtained. In particular, the extracts of blackberry, red mulberry, black rice, When the Seomyeongtaeae extract is used together with the banana extract, it is possible to remove the bitter flavor of the seaweed and the stinginess of the Seomyeonae according to the above range, and to provide a food composition having high flavor whilst chewing, .

When the above-mentioned cherry extract is contained in an amount of less than 11 parts by weight, the flavor of the banana extract and the cloudy cherry flavor are entirely smoothed. When the extract is provided as a complex extract containing banana extract, There is no problem. In addition, when the content is more than 20 parts by weight, the flavor and sourness of the cherry is strengthened, and the sweetness and flavor of the banana are not well mixed with each other, and the harmonious flavor can not be mixed with the overall flavor.

When the above-mentioned Ganoderma extract is mixed at a ratio of less than 8 parts by weight, the banana fragrance remains as a whole with a pale flavor, and the palatable flavor of the ganoderma is not felt. In addition, even when it is provided as a complex extract containing a banana extract, there is a problem that the fragrance of the cold water is not removed. In addition, when the above-mentioned extract is added in an amount of more than 20 parts by weight, the fragrance of the lotion becomes stronger, and the fragrance of the fragrance is used as a whole, and the palatability is lowered.

The above vine is a deciduous vine plant with daphnia plant, and is effective in removing the bitter taste of the daphnia and the stinginess of the vinegar when the vinegar extract is used. However, when the extract is used in an amount of less than 1 part by weight, it remains as a flat aroma together with the banana as a whole. When the extract is used as a complex extract containing a banana extract, it neutralizes the bitter taste of the above- I can not. On the other hand, if it exceeds 5 parts by weight, the fragrance of the crane itself becomes stronger and the smoother as a whole becomes stronger, resulting in poor palatability.

Therefore, it is advantageous to provide a functional food which has high palatability and can prevent white fever and promote black cohosh if it is in the above range.

The cherry extract, the Phellinus linteus extract, and the Crane extract may be ethanol extracts. When the ethanol extract is used, it may be advantageous to achieve the above effect by mixing with the composition for preventing gray hair and promoting the induction of gray hair.

The composition for preventing hair gray and promoting hair growth according to another embodiment of the present invention may include the composition for preventing gray hair and promoting hair growth.

The composition for external use of hair according to the present invention refers to a composition for external application which is provided in the form of being sprayed on hair or the like for functional or aesthetic purposes and is used for hair cosmetic compositions such as hair essence, hair nutrient solution, hair cream, hair lotion, Hair sprays and the like are included here, and the present invention is not limited to the above listed products.

The composition for preventing hair gray and for promoting hair growth according to another embodiment of the present invention may include the composition for preventing gray hair and promoting hair growth.

The hair cleansing composition according to the present invention refers to a composition used for the purpose of washing or washing hair, and includes shampoo, rinse, hair soap, hair cleaner and the like, and is not limited to the above listed products.

Preferably, the composition for hair external application and the composition for hair cleansing comprising the banana comprise 1 to 10 parts by weight of dandelion, 1 to 5 parts by weight of mulberry crumbs and 5 to 10 parts by weight of grape seed extract based on 100 parts by weight of the composition for preventing gray hair, Weight part may be further included.

When the above-mentioned dandelion extract, mallow vine extract and grape seed extract are contained, it is possible to protect the scalp and the hair to improve the health of scalp and hair, and it is also advantageous in that an excellent fragrance can be obtained by using excellent natural materials .

When the above-mentioned dandelion extract is contained in an amount of less than 1 part by weight, there is a problem that the flavor enhancing effect of the external preparation composition or the cleaning composition as a whole is low and the effect of protecting the scalp is low. In addition, when the amount of the dandelion extract is more than 10 parts by weight, the strong dandelion flavor is not well mixed, and the content of the dandelion extract is relatively increased, thereby reducing the scalp and hair improving effect.

When the above-mentioned vinegar extract is used in the above-described range, the effect of eliminating the stinginess of the vinegar can be obtained.

When the grape seed extract is used in an amount of less than 5 parts by weight, the effect of improving the scalp and hair is lowered. When the amount of the grape seed extract is more than 10 parts by weight, the oil is relatively increased, And the composition for accelerating the induction of hair loss may interfere with removal of the H ₂ O ₂ DPPH radical of the hair follicle.

Accordingly, it may be advantageous to provide a functional hair external preparation or a functional hair cleansing composition which is superior in flavor and has high perfume and hair improving effect, as well as being capable of preventing white fever and promoting the induction of black curl according to the above range.

The above-mentioned Dandelion Extract, Crassulaceae Extract and Grape Seed Extract may be ethanol extracts. When the ethanol extract is used, it may be advantageous to achieve the above effect by mixing with the composition for preventing gray hair and promoting the induction of gray hair.

The method for preparing a banana-containing composition for preventing and inhibiting gray hair according to another embodiment of the present invention comprises drying and pulverizing a banana, adding 10 to 99% by weight of an ethanol solvent and the banana pulverized product at a ratio of 10: 1 to 1: 1 part by weight to prepare a banana extract; Preparing a black sugar extract by mixing the ethanol solvent of 10 to 99% by weight with the powder of the black starch at a ratio of 10: 1 to 1: 1 by weight after drying and pulverizing the black sugar; A step of preparing an aqueous extract of sodium hypochlorite by mixing and drying 10 to 99% by weight of an ethanol solvent and the above-mentioned aqueous sodium hydroxide solution at a ratio of 10: 1 to 1: 1 by weight; Preparing a black rice extract by mixing and drying the black rice with 10 to 99% by weight of ethanol solvent and the black rice powder at a ratio of 10: 1 to 1: 1 by weight; Preparing a brown rice extract by mixing and drying the brown rice with 10 to 99% by weight of an ethanol solvent and the brown rice powder at a ratio of 10: 1 to 1: 1 by weight; Preparing an extract of Seomyeok-myeon, which is prepared by drying and crushing Seomyo-myeon, mixing 10 to 99% by weight of an ethanol solvent with the above-mentioned Seomye-tai pulverized product at a ratio of 10: 1 to 1: 1; In addition, the method may include a step of mixing the banana extract, the black mangrove extract, the red manganese extract, the black rice extract, the brown rice extract and the western blotting extract.

The composition for preventing gray hair and preventing hair growth according to the present invention comprises a banana extract and prevents white fever by using a natural composition showing the ability of scavenging H ₂ O ₂ DPPH radical and reducing the active oxygen, .

Particularly, the composition for prevention of gray hair and the composition for promoting the induction of black hair can be provided with a composite composition as an extract composition derived from a natural product, so that it is possible to achieve a significant effect on prevention of gray hair and promotion of black hair induction without side effects of skin or hair damage.

In addition, the hair external preparation for preventing gray hair and promoting hair growth according to the present invention may contain banana extract in a certain range, and thus can be used as an external preparation for hair through excellent flavor together with prevention of gray hair and promotion of black hair induction. And can be produced by using the composition for preventing gray hair and for promoting black cohosh.

Further, the present invention provides a method for producing the composition for preventing gray hair and for promoting gray hair induction.

FIG. 1 is a graph showing DPPH radical scavenging ability and reducing power of a composition for preventing gray hair and promoting gray hair induction according to an embodiment of the present invention.
FIG. 2 is a graph showing the results of inhibition of intracellular H ₂ O ₂ production in a composition for preventing gray hair and promoting the induction of gray hair according to an embodiment of the present invention.
FIG. 3 is a graph showing the results of experiments on the production of melanin in B16F1 cells of a composition for preventing gray hair and promoting the induction of gray hair according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

[Production of materials and samples]

Dulbecco's Modified Eagle's Medium (DMEM), Trypsin-EDTA, penicillin (10,000 U / ml) / streptomycin (10,000 μg / ml) / amphotericin (2,500 μg / ml) and fetal bovine serum , Life Technologies (Paisley, Scotland). B16F1 cell line was purchased from ATCC (American Type Culture Collection, USA). α-MSH, imatinib mesylate, IBMX, MTT reagent and other reagents were purchased from Sigma Chemical Co. (St. Louis, MO, USA).

[ Manufacturing example : Preparation of extract]

According to the method of the present invention for preventing the gray fever and promoting the induction of hair growth, the banana skin, the black rice, the red mulberry, the black rice, the brown rice, and the western blot are extracted for 3 days each with 80 wt% ethanol as a solvent, filtered, (B), black rice extract (C), brown rice extract (D), Seomyeongae extract (E) and banana pulp extract (S2) were prepared.

Each of the above extracts was prepared as a single extract (EXCS) and a mixed extract (MIXEE) as shown in Table 1 below.

S1 A B C D E S2 EXCS 1 100 - - - - - - EXCS 2 - 100 - - - - - EXCS 3 - - 100 - - - - EXCS 4 - - - 100 - - - EXCS 5 - - - - 100 - - EXCS 6 - - - - - 100 - EXCS 7 - - - - - - 100 MIXEE 1 100 150 30 - - - MIXEE 2 100 250 80 - - - - MIXEE 3 100 350 110 - - - - MIXEE 4 100 450 200 - - - - MIXEE 5 100 350 110 50 50 - - MIXEE 6 100 350 110 90 90 - - MIXEE 7 100 350 110 150 150 - - MIXEE 8 100 350 110 200 200 - - MIXEE 9 100 350 110 150 150 30 - MIXEE 10 100 350 110 150 150 50 - MIXEE 11 100 350 110 150 150 100 - MIXEE 12 100 350 110 150 150 200 - MIXEE 13 - 350 110 150 150 100 100

(Unit: parts by weight)

[ Experimental Example  1: of banana extract Melanosite  Cell activity measurement]

In order to confirm the basic effect of the extract composition of the present invention, the following experiment was conducted. In the following experiment, EXCS 1 was used to test the promoting effect of black hair induction in banana skin extract (S1).

At the time of hair transplantation, the scalp containing the hair follicles was taken from the occipital area. The hair follicles were removed by removing the surrounding tissues as much as possible. The hair follicle was removed and the upper part of the hair follicle was removed while confirming the area where the sebaceous gland was attached. The lower half of the hair follicle was extracted with 200 nM Phorbol myristate acetate The first cultured melanocyte cells were removed with 0.25% Trypsin / EDTA in DMEM (Dulbeccos Modification of Eagles Medium) medium containing 10% fetal bovine serum, and the number of cells was counted with a hemocyte counter, (1 × 10 ⁴ cells / well) in each well of a 24-well plate. After incubation for 1 day at 37 ° C in a 5% CO 2 and 100% humidity incubator, saline was added to the control group and EXCS 1 was added to the experimental group at 5, 1, 0.5, 0.1, 0.05, 0.01, 0.005 and 0.001 Lt; / RTI > and then incubated for 7 days. After each lapse of time, 300 μl of a solution of MTT (3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazolium bromide, Sigma Chemical Co., USA) dissolved in physiological saline was added well and cultured for 4 hours. After culturing, the medium was removed and formazan crystals formed by adding 200 μl of DMSO (dimethyl sulfoxide, Junsei, Japan) were dissolved. Then, the cells were transferred to a 96-well plate for cell activity and analyzed using an ELISA analyzer (Spectra MAX 250, Co., USA) at 540 nm. The cell activity of the control group treated with EXCS 1 was regarded as 100% and the cell activity was expressed as% in comparison with the experimental group.

Banana peel extract Control group 0.001% 0.01% 0.05% 0.1% 0.5% One% 10% Cell activity (%) 100 120% 135% 165% 170% 175% 185% 195%

(Unit:% of the control group)

Referring to Table 2, it can be seen that when the banana bark extract is used, the cell activity of melanocytes is increased as the concentration thereof is increased. Therefore, it can be seen that when banana peel extract is used, the melanocyte activity can be increased to produce a black hair-inducing effect.

[ Experimental Example  2: Test for promoting melanin biosynthesis of banana extract]

At the time of hair transplantation, the scalp containing the hair follicles was collected from the occipital area, and the surrounding tissue was removed to remove the hair follicle. The hair follicle was removed and the upper part of the hair follicle was removed while confirming the area where the sebaceous gland was attached. The lower half of the hair follicle was extracted with 200 nM Phorbol 12-myristate The first cultured melanocyte cells from the hair were removed with 0.25% Trypsin / EDTA in a DMEM medium containing 13-acetate and 10% fetal bovine serum. × 10 ⁵ , and cultured at 5% CO _{2 at} 37 ° C. until cells are attached to the bottom of the wells at about 80% or more. After culturing for 1 day, the precursor of the melanin pigment was doped with 0.08 mM, and then the banana bark extract was added at the test concentrations of 5, 1, 0.5, 0.1, 0.05, 0.01, 0.005 and 0.001 wt% Saline was added to the control group. Cells from which the medium has been removed are washed with PBS (phosphate buffer saline), and treated with trypsin to recover the cells. The recovered cells are counted using a hematocytometer, centrifuged at 5,000 to 10,000 rpm for 10 minutes, and the supernatant is removed to obtain a pellet. The cell pellet is dried at 60 ° C., and then 100 μl of 1 M sodium hydroxide solution containing 10% DMSO or an appropriate amount of cell lysis buffer is added to obtain intracellular melanin in a 60 ° C. thermostat. The absorbance at 490 nm was measured with a microplate reader. The content of melanin in the cells was expressed as% in comparison with the control group not treated with sodium phenylbutyrate and the experimental group. The results are shown in Table 3 below.

Banana peel extract Control group 0.001% 0.01% 0.05% 0.1% 0.5% One% 10% Melanin content (%) 100% 120% 145% 160% 175% 180% 195% 210%

(Unit:% of the control group)

Referring to Table 3, it can be seen that melanin biosynthesis of melanocytes is promoted depending on the concentration of the banana bark extract when it is used. Therefore, when banana peel is used, melanin biosynthesis can be promoted to prevent gray hair and to induce a black blood cell-producing effect.

[ Experimental Example  3: Comparison by single extract]

One: DPPH  radical Minority  For reducing power MIXEE  Effect of 3

In order to investigate the antioxidant effect of each extract (EXCS) in this experiment, first, the ability of DPCS radical scavenging activity of EXCS 1 to 7 was examined.

Brand-Williams (Han, K.-H., Oh, J.-C. and Ryu, C.-H. 2004. A study on the preparation of germinated brown rice gruel. Journal of the Korean Society of Food Science and Nutrition 33, 1735-1741.) Experimental methods were modified to measure the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability of EXCS 1-7. Each sample was mixed at the same concentration, mixed well for 10 seconds, reacted at room temperature for 20 minutes, and absorbance was measured at 525 nm. The DPPH radical content was expressed as% of the absorbance ratio of the sample added group and the control group.

 As a result, as shown in FIG. 1, EXCS 1 showed the best DPPH radical reduction effect, and it was confirmed that the EXCS 1 had almost the same effect as the positive control vitamin C.

2. D cell  of mine H ₂ O ₂  Effect on inhibition of production

When DCFH-DA reacts with H ₂ O ₂ in B16F1 cells, DCF is generated and fluoresced. DCFH-DA was prepared at a concentration of 20 μM in DMEM medium, treated with B16F1 cells, and left at 37 ° C. for 20 minutes to infiltrate DCFH-DA into the cells. The negative control, 0.1% vitamin C, and each EXCS were treated with test concentrations for 1 hour. After washing 4 times with PBS, 100 μl of HBSS buffer solution containing 250 μM H ₂ O ₂ was added to the cells. In the cell, H ₂ O ₂ reacts with DCFH-DA to produce fluorescent DCF. The fluorescence was measured using a multi-detection microplate reader (SynergyHtx) under excitation: 488 nm and emission: 530 nm.

Fluorescence measurements were performed to determine how much H ₂ O ₂ was removed from each single extract. FIG. 2 shows that fluorescence of the control group was increased by 100% or more compared to the blank test group, and EXCS 1 showed the best ability to eliminate H ₂ O _{2 in} the cells.

2. Reversibility Assay

Oyaizu (Kim, S.-R., Ahn, J.-Y., Lee, H.-Y. and Ha, T.-Y. 2004. Various properties and phenolic acid contents of rices and rice brans with different milling fractions , Korean Journal of Food Science and Technology 36, 930-936). To 1 ml of MIXEE 3, 200 mM phosphate buffer solution of pH 6.6 and 1% potassium ferricyanide were added in the order of 1 ml each, and the mixture was stirred for 20 minutes in a water bath at 50 ° C. 1 ml of 10% TCA solution was added and 1 ml of supernatant obtained by centrifugation at 13,500 × g for 15 minutes was added to 1 ml of distilled water and ferric chloride, and the absorbance was measured at 700 nm. 0.01% vitamin C was used as a positive control. The reducing power of the sample was expressed as% value of the absorbance ratio of the sample addition group and the control group.

3. B16F1  Effect on intracellular melanin production

To investigate the effect of each single extract on melanin production via DOPA stimulation, melanin content was measured using B16F1 cells. Cells were seeded at 3 × 10 5 cells / well in a 6-well plate, and melanin production was stimulated with L-DOPA 1 hour later. Cells were cultured for 48 hours. Cells were collected and centrifuged at 1,200 rpm for 5 minutes to precipitate and then dissolved in 1 ml homogenization buffer (50 mM sodium phosphate pH 6.5, 1% Triton X-100, 2 mM phenyl methyl sulfonyl fluoride). To the pellet obtained, 200 μl of 1 N NaOH (10% DMSO) was added and the absorbance was measured at 405 nm after vortexing. The amount of melanin produced in each well was calculated using a standard calibration curve obtained from melanin standard (Sigma, USA). Melanin compares melanin production in unit cells (104 cells).

As a result, as shown in FIG. 3, it can be seen that each single extract has a positive effect on melanin production overall. EXCS 1 has the best effect, Respectively.

[ Experimental Example  4: Test for compound extract]

(MIXEE 1 to 13) and the comparative examples (COMP 1 to 4) were administered at the same concentration of 16 μg in order to ascertain the synergistic effect according to the use of the compound extract and the content of each compound, And the following experiment was conducted. The specific experimental method was the same as that of Experimental Example 1 described above.

Further, in order to directly compare the data of EXCS 1 with that of EXCS 1 which was tested in Experimental Example 1, the data value of EXCS 1 was fixed to 100 in each experiment, and the results of the above Examples (MIXEE 1 to 13) and The comparative examples (COMP1 to COMP4) were expressed as ratios so that the results could be directly compared.

1. Effects on DPPH radical scavenging ability and reducing power

Experiments were carried out in the same manner as in Experimental Example 3, and the results were compared with EXCS 1, expressed as a ratio, and the results are shown in Table 4 below. The results below show that DPPH radical scavenging ability is better as the number is lower, and the higher the number, the better the reducing power.

MIXEE 1 MIXEE 2 MIXEE 3 MIXEE 4 MIXEE 5 MIXEE 6 MIXEE 7 MIXEE 8 MIXEE 9 MIXEE 10 MIXEE 11 MIXEE 12 MIXEE 13 Scatters 100 105 110 89 90 100 113 102 99 105 118 112 78 Reducing power 97 110 114 90 95 102 118 98 103 104 122 55 56

(Unit: index)

2. In vitro melanin synthesis  effect

Experiments were carried out in the same manner as in Experimental Example 1, and the results were compared with EXCS 1, expressed as a ratio, and the results are shown in Table 5 below. The following results show that the higher the number, the better the melanin synthesis effect.

MIXEE 1 MIXEE 2 MIXEE 3 MIXEE 4 MIXEE 5 MIXEE 6 MIXEE 7 MIXEE 8 MIXEE 9 MIXEE 10 MIXEE 11 MIXEE 12 MIXEE 13 Melanin production 98 103 108 98 97 105 117 103 89 107 127 110 66

(Unit: index)

3. Intracellular H ₂ O ₂  Production inhibitory effect

Experiments were carried out in the same manner as in Experimental Example 1, and the results were compared with EXCS 1, expressed as a ratio, and the results are shown in Table 6 below. The following results show that the lower the number is, the better the effect of inhibiting H ₂ O ₂ formation.

MIXEE 1 MIXEE 2 MIXEE 3 MIXEE 4 MIXEE 5 MIXEE 6 MIXEE 7 MIXEE 8 MIXEE 9 MIXEE 10 MIXEE 11 MIXEE 12 MIXEE 13 DCF fluorescence 110 91 56 81 76 64 50 56 64 54 44 53 120

(Unit: index)

4. Experiments to stimulate black blood

Examples of healthy female and male subjects aged 40 to 50 suffering from a large number of gray hair were selected for each of the examples and comparative examples randomly according to each composition of the present invention, and on the basis of both head and top parts, Example (MIXEE 1 to 13) was applied for two months in the morning and evening, twice a day. The inhibition of gray hair formation and the evaluation of the effect of promoting black hair induction were evaluated by subject subjective evaluation after using the composition of the present invention by evaluating the degree of inhibition of white hair formation through the following evaluation scores. The results are shown in Table 7 below.

Number of people 0 point 1 point 2 points 3 points MIXEE 1 One 4 2 3 MIXEE 2 One 5 4 MIXEE 3 One 4 5 MIXEE 4 2 5 3 MIXEE 5 3 4 3 MIXEE 6 3 2 5 MIXEE 7 One 2 7 MIXEE 8 2 3 5 MIXEE 9 One 3 6 MIXEE 10 One 3 3 5 MIXEE 11 One 4 8 MIXEE 12 4 One 5 MIXEE 13 3 2 One 3

(Score)

3: The number of white stones after coating was reduced to 90% or less, relative to the number of white stains before application was started.

2: The number of white streaks after coating was reduced to 60% or less, relative to the number of white streaks before application was started.

1: The number of gray hair after coating was reduced to 30% or less, relative to the number of white hair before application was started.

0: The gray hair did not decrease at all.

Because of the decrease of catalase, methionine sulfoxide reductase A and B protein expression due to the change of black hair into white moth as the aging progresses, H ₂ O ₂ accumulates around the hair follicle, and methionine residue of tyrosinase involved in melanin synthesis Oxidizes the enzyme structure, which results in a decrease in melanin synthesis, resulting in white moth.

Therefore, it can be confirmed that the example according to the present invention is excellent in antioxidative effect, promotes melanin synthesis, and can effectively remove H ₂ O ₂ . Especially, the results of MIXEE 2, 3, 6, 7, 11 and 12 were found to be very good. In particular, it was confirmed that the most excellent effect is obtained in the evaluation of the actual inhibition of gray hair.

On the other hand, in the skin melanocyte, melanin production is increased by stimulating with active oxygen, since the whole process of tyrosine is produced by melanin. Therefore, treatment with antioxidants causes a reduction process to inhibit the production of melanin, which leads to a skin whitening effect. On the other hand, in hair melanocytes, melanin is continuously produced irrespective of the stimulation of reactive oxygen species, resulting in black hair. When the aging if the enzyme activity of tyrosinase in the follicular cell H ₂ O ₂ are due to the accumulation and due to the inhibited melanin synthesis is reduced handling antioxidants enters aunt to aunt to remove the H ₂ O ₂ to be aunt switches Moro Black There is a possibility to be able to. In this study, MIXEE normalized the tyrosinase enzyme by removing H 2 O 2 accumulated in the hair follicles and promoted melanin production. In order to support this reasoning, H ₂ O ₂ was treated in the cells to examine the antioxidant activity of MIXEE in the cells, and the excellent H ₂ O ₂ removal efficiency was confirmed by confirming the above reasoning.

[Manufacturing Example 1] Shampoo composition 1

Category (% by weight) Production Example 1 Production Example 2 Production Example 3 Production Example 4 Sodium lauryl sulfate (30%) 20.0 20.0 20.0 20.0 Polyoxyethylene lauryl
Sodium sulfate (30%) 20.0 20.0 20.0 20.0 Coconut oil fatty acid diethanolamide 2.0 2.0 2.0 2.0 Propylene glycol 2.0 2.0 2.0 2.0 The composition for preventing gray hair and promote Japanese black causes, including banana water 0.001 0.01 0.1 One Color (Blue No. 1) Suitable amount Suitable amount Suitable amount Suitable amount Paraoxybenzoic acid ester 0.2 0.2 0.2 0.2 Spices (Combination Incense SKP-3473) Suitable amount Suitable amount Suitable amount Suitable amount Citric acid Suitable amount Suitable amount Suitable amount Suitable amount Purified water Balance Balance Balance Balance

 (Unit: wt%)

[ Manufacturing example  2] Hair dye Post treatment agent  Composition

Ingredients Production Example 5 Example 6 Liquid paraffin 3.00 8.00 Cetostearyl alcohol 2.00 0.60 Behentrimonium chloride 1.0 2.5 Methyl paraben 0.15 2.5 A composition for prevention of gray hair including banana and a composition for promoting black cohosh 0.001 One Combination incense Suitable amount Suitable amount Purified water Balance Balance

 (Unit: wt%)

[ Manufacturing example  3] hair  Gel composition

Ingredients Production Example 7 Production Example 8 Production Example 9 Production Example 10 A composition for prevention of gray hair including banana and a composition for promoting black cohosh 0.001 0.01 0.1 One ethanol 10 10 10 10 Acrylate-based polymer 2 3 4 5 Polyvinylpyrrolidone 0.2 0.2 0.2 0.2 Carboxyvinyl polymer 0.3 0.3 0.3 0.3 Triethanolamine 2 3 4 5 incense 0.2 0.2 0.2 0.2 Purified water Balance Balance Balance Balance

 (Unit: wt%)

[Preparation Example 4] Preparation of ointment

(0.01%), 0.01% of corsecetin (Sigma), 8.0% of diethyl sebacate, 5.0% of salicylic acid, 6.0% of polyoxyethylene oleyl-ether phosphate (EO 4) , An appropriate amount of paraoxybenzoic ester, and a residual amount of vaseline, according to a conventional ointment preparation method.

[Preparation Example 5] Preparation of softening longevity

The composition for prevention of gray hair including bananas and the composition for promoting gray hair induction was diluted with 0.01% of coercetin, 0.01% of glycerin, 1.0% of hyaluronic acid, 0.1% of polyoxyethylene oleyl ether (EO20), polyoxyethylene hydrogenated castor oil (EO40 ) 0.1%, a p-hydroxybenzoic acid ester proper amount, a perfume proper amount, a pigment suitable amount, and a purified water residual amount.

[Preparation Example 6] Preparation of nutritional milky lotion

The composition for prevention of gray hair including banana and the composition for promoting black feather induction was formulated in an amount of 0.01%, 0.01% of coercetin, 1.0% of cetanol, 0.5% of wax, 2.0% of vaseline, 6.0% of squalane, 3.0% of ethanol, %, Polysorbate 60 1.0%, sorbitan sesquioleate 0.3%, carboxyvinyl polymer 0.3%, triethanolamine 0.3%, p-hydroxybenzoic acid ester suitable amount, coloring matter proper amount, purified water remaining amount . ≪ / RTI >

[Preparation Example 7] Preparation of cream

The composition for prevention of gray fever and the composition for promoting gray hair induction comprising bananas, which comprises 0.01%, stearyl alcohol 6.0%, stearic acid 2.0%, concentrated glycerin 1.0%, squalane 9.0%, 1,3-butylene glycol 6.0%, polysorbate 60 1.5 %, Polyethylene glycol 1000 4.0%, hardened lanolin 4.0%, octyldodecanol 10.0%, sorbitan stearate 0.8%, triethanolamine 0.5%, p-hydroxybenzoic acid ester suitable amount, pigment suitable amount, purified water residual amount Can be produced according to the method.

[Preparation Example 8] Preparation of essence

A mixture of cyclomethicone 15.0%, caprylic capric triglyceride 3.0%, mineral oil 3.0%, wax 1.0%, cetyl dimethicone copolyol 3.0%, MIXEE 1.0%, resveratol 0.001%, glycerin 5.0%, magnesium sulfate 3.0 %, An appropriate amount of paraoxybenzoic acid ester, an appropriate amount of perfume, a suitable amount of pigment, and a remaining amount of purified water.

[Preparation Example 9] Food composition

Using the above-mentioned Example MIXEE 11, a natural composition was prepared by mixing natural products as shown in Table 11 below. The above-mentioned tea composition can be prepared by a conventional method.

Manufacturing example
9-1 Manufacturing example
9-2 Manufacturing example
9-3 Manufacturing example
9-4 Manufacturing example
9-5 Manufacturing example
9-6 Manufacturing example
9-7 Manufacturing example
9-8 Manufacturing example
9-9 MIXEE 11 100 100 100 100 100 100 100 100 100 Cherry extract - 15 - - 5 11 15 20 30 Bengal extract - - 10 - 4 8 12 20 30 Vine Extract - - - 5 0.5 One 3 5 7

(Unit: parts by weight)

10 persons were arbitrarily selected in the preparation example according to Table 9, and after tasting, the flavor and taste were subjectively evaluated and expressed as an index of 1 to 10. The results are shown in Table 10 below. The indexes shown in Table 12 below are higher in aroma and taste. On the other hand, palatability is defined as an average value of the flavor and taste.

Manufacturing example
9-1 Manufacturing example
9-2 Manufacturing example
9-3 Manufacturing example
9-4 Manufacturing example
9-5 Manufacturing example
9-6 Manufacturing example
9-7 Manufacturing example
9-8 Manufacturing example
9-9 incense 3.1 6.6 4.5 4.5 6.1 8.3 8.8 8.5 6.7 flavor 4.5 6.1 5.1 3.9 5.2 7.9 7.5 8.1 5.8 Likelihood 3.8 6.4 4.8 4.2 5.7 8.1 8.2 8.3 6.3

Referring to Table 12, it can be seen that the flavor and taste are superior in the ranges of Production Examples 9-6 to 9-8. Therefore, when a food composition having high palatability according to the above range is provided, it may be advantageous to supply a functional food composition for promoting the induction of the black feather.

[Production Example 10] Shampoo composition 2

In Table 8 of the shampoo composition of Preparation Example 1, a shampoo composition was prepared using the composition consisting of the fragrance and the following Table 13. The other compositions were the same.

Manufacturing example
10-1 Manufacturing example
10-2 Manufacturing example
10-3 Manufacturing example
10-4 Manufacturing example
10-5 Manufacturing example
10-6 Manufacturing example
10-7 Manufacturing example
10-8 Manufacturing example
10-9 MIXEE 11 100 100 100 100 100 100 100 100 100 dandelion
extract - 5 - - 0.5 One 5 10 15 Vine Extract - - 5 - 0.5 One 3 5 7 Grape seed extract - - - 5 3 5 7 10 15

(Unit: parts by weight)

Ten persons using the hair wax product were arbitrarily selected and used for one month, and then the washing power, the skin irritation, the hair elasticity and the fragrance were subjectively evaluated to obtain an index of 1 to 10 And the results are shown in Table 14 below. The washing power index shown in the following Table 12 shows that the higher the number, the better the effect, and the lower the number of irritation index and perfume index, the better the effect.

Manufacturing example
10-1 Manufacturing example
10-2 Manufacturing example
10-3 Manufacturing example
10-4 Manufacturing example
10-5 Manufacturing example
10-6 Manufacturing example
10-7 Manufacturing example
10-8 Manufacturing example
10-9 Cleaning power 8.6 8.1 8.1 7.9 8.3 7.8 8.3 9.1 9.1 Skin irritation 6.4 5.6 6.5 5.7 5.4 2.8 2.1 3.4 4.9 Hair Stimulation 5.5 5.5 7.8 5.7 6.1 3.7 4.1 4.2 5.2 incense 3.1 4.4 5.1 4.8 4.1 3.1 2.4 3.6 5.1

Referring to Table 14, it can be seen that, in Examples 10-1 to 10-9, there is no synergistic effect on detergency according to the use of dandelion, milky vine, and grape seed extract. but. It can be confirmed that the skin irritation is small and the hair irritation is small in the range of Production Examples 10-6 to 10-8, so that the scalp or hair damage due to use of the shampoo can be suppressed. Also, it has been confirmed that the composition can be advantageously used as a composition for hair external application or hair cleansing composition comprising the composition for prevention of gray hair and the composition for promoting hair growth, which is relatively excellent in the above range.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (9)

Contains banana peel extract
The solvent of the banana bark extract is ethanol
A composition for preventing gray hair and for promoting black hair induction. The method according to claim 1,
Blackberry extract and
Which further comprises an extract
A composition for preventing gray hair and for promoting black hair induction. The method according to claim 1,
Black rice extract and
It contains more brown rice extract.
A composition for preventing gray hair and for promoting black hair induction. The method according to claim 1,
Blackberry extract,
Red pepper extract,
Black rice extract,
Brown rice extract and
Which further contains Seomyeongae extract
A composition for preventing gray hair and for promoting black hair induction. 5. The method of claim 4,
The blackberry extract, the red mulberry extract, the black rice extract, the brown rice extract and the Seomyeok mackerel extract were prepared by extracting 10 to 99% by weight of ethanol with a solvent
A composition for preventing gray hair and for promoting black hair induction. A composition for promoting the prevention of gray hair and a composition for promoting hair growth according to any one of claims 1 to 5
Food composition for prevention of gray hair and promotion of black cohosh. A composition for promoting the prevention of gray hair and a composition for promoting hair growth according to any one of claims 1 to 5
A composition for external use of hair for preventing gray hair and promoting hair growth. A composition for promoting the prevention of gray hair and a composition for promoting hair growth according to any one of claims 1 to 5
A composition for hair bleaching prevention and prevention of gray hair. Preparing a banana peel extract by mixing the 10 to 99% by weight ethanol solvent and the banana peel powder with 10: 1 to 1: 1 part by weight of the banana peel;
Preparing a black sugar extract by mixing the ethanol solvent of 10 to 99% by weight with the powder of the black starch at a ratio of 10: 1 to 1: 1 by weight after drying and pulverizing the black sugar;
A step of preparing an aqueous extract of sodium hypochlorite by mixing and drying 10 to 99% by weight of an ethanol solvent and the above-mentioned aqueous sodium hydroxide solution at a ratio of 10: 1 to 1: 1 by weight;
Preparing a black rice extract by mixing and drying the black rice with 10 to 99% by weight of ethanol solvent and the black rice powder at a ratio of 10: 1 to 1: 1 by weight;
Preparing a brown rice extract by mixing and drying the brown rice with 10 to 99% by weight of an ethanol solvent and the brown rice powder at a ratio of 10: 1 to 1: 1 by weight;
Preparing an extract of Seomyeok-myeon, which is prepared by drying and crushing Seomyo-myeon, mixing 10 to 99% by weight of an ethanol solvent with the above-mentioned Seomye-tai pulverized product at a ratio of 10: 1 to 1: 1; And,
The method comprising the step of mixing the banana bark extract, the black sugar extract, the red mulberry extract, the black rice extract, the brown rice extract,
A method for the prevention of gray hair and for promoting the induction of black cohosh.

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