KR20170048904A - Cosmetic compositions for skin whitening containing costunolide from natural extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening comprising a cosuronolide as an active ingredient, wherein the cosuronolide is obtained by separation and purification through extraction of a natural product, wherein the cosuronolide is a plant, ≪ / RTI >
The above-mentioned course tololide according to this nickname has an effect of inhibiting melanin formation and improving pigment deposition caused by ultraviolet rays, and thus the composition containing the course tonolide can be usefully used as a skin whitening cosmetic composition.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a cosmetic composition for skin whitening containing costunolide from natural extracts,

The present invention relates to a cosmetic composition for skin whitening comprising a cosuronolide as an active ingredient, and more particularly, to a cosmetic composition containing a cosuronolide as an active ingredient, which can safely be used without side effects on the skin, To inhibit pigment deposition such as spots and freckles, and to provide a cosmetic composition for skin whitening which is excellent in skin whitening effect.

Skin is an important tissue that protects the human body in direct contact with the external environment and exhibits biochemical and physical functions. This tissue is composed of three major parts: epidermids, dermis and hypoglossal tissue, and it mainly acts as a barrier to protect the human body. Melanin is a polymeric substance of phenols widely distributed in nature, and it is an important pigment that determines the color of skin and exists in the epidermis of skin. Eumelanin is mainly black or brown, and pheomelanin is yellow or red. Melanin is produced from the melanocytes in the basal layer of the epidermis. The synthesized melanin is removed through the keratinocyte keratinization process, moving to an epidermal cell called keratinocyte.

Melanin, which determines the color of human skin, is a kind of pigment that is a polymeric substance of phenols existing in animals, plants and microorganisms and has a protective function against external environment such as ultraviolet ray, drying and extreme temperature. Melanin is a substance It is biosynthesized by continuous oxidation of the tyrosinase enzyme in the intracellular melanosome of the existing melanocyte.

However, in the skin exposed to the sunlight, the melanin-producing reaction is promoted by tyrosinase to prevent the damage of the cells or tissues in the skin due to ultraviolet rays and the progress of photoaging due to ultraviolet rays, and excessive melanin is produced, Spots, freckles, black spots and so on. This melanin is mainly oxidized to L-DOPA and DOPAquinone by tyrosine in Tyrosine, and then converted to 5,6-dihydroxy-indole, indole-5, (Pawelk JM and Korner AM, Am. Sci., 70, p136, 1982, Butt VS, Recent Adv., Phytochemistry), which is oxidized by oxidation with indol-5,6-quinone , 12, p433, 1979, Lopez JNR, J. Tudela, R. Varon, FG Carmona, FG Canovas, J. Biol. Chem., 267, p3901-3910, 1992).

That is, the biosynthesis of melanin is inhibited by a method of preventing ultraviolet exposure of skin, inhibiting activity of tyrosinase, reducing melanin, promoting discoloration and excretion of the produced melanin, inhibiting metabolism and proliferation of melanocytes . In addition, a new melanin biosynthetic pathway was found to be associated with this skin whitening, and several new regulators such as TRP-1, TRP-2 and MSH were found in addition to tyrosinase, (Slominski A, Tobin DJ, Shibahara S, Wortsman J, Physiol Rev, 84 (4), p1155-1228, 2004) ; Land EJ, Ramsden CA, Riley PA, Mthods Enzymol, 378, p88-109, 2004; Kameyama K, Jimenez M, Muller J, Ishida Y, Hearing VJ, Differentiation 42 RA, Melanoma Res, 12 (5), p405-416, 2002).

The skin color of a person is determined by various factors such as the activity of melanocyte making melanocyte, the distribution of blood vessels, the thickness of skin and the presence or absence of pigment inside and outside the body such as carotenoids and bilirubin, among which melanocytes 10-1236212 The most important is melanin, a melanin produced by the action of various enzymes such as thyroxinase. The formation of melanin pigment is affected by environmental factors such as genetic factors, hormonal secretion, physiological factors such as stress, and ultraviolet irradiation. Melanin is present in the outer skin layer of the skin to protect the skin organs below the dermis from ultraviolet rays and at the same time it plays an important role in protecting the proteins and genes in the skin by catching the free radicals generated in the skin. It is known that it not only forms spots and freckles by overproduction, but also promotes skin aging and plays an important role in skin cancer induction.

In recent years, Asian women prefer white, clean skin like white whiting, and it is important for the beauty of the beauty. Therefore, development of whitening agent for the treatment of skin pigmentation abnormality and the desire for beauty is being actively carried out. In the development of a whitening agent, there is known a method of reducing the produced melanin pigment by decoloring and a method of inhibiting the tyrosinase activity, which is an enzyme that forms a melanin pigment. However, it is known that the whitening agent using tocopherol or vitamins used for reducing the melanin pigment has a very small discoloring effect of the melanin pigment. Accordingly, inhibitors that inhibit the activity of tyrosinase and inhibit the production of melanin pigment have been attracting attention. Especially, plant natural products are more safe than synthetic raw materials and have various beneficial ingredients, and their images are good and they are a good target of cosmetic raw materials.

The melanin pigment produced in melanocytes of body skin is a phenolic polymer substance having a complex form of black pigment and protein. It protects the subcutaneous skin organs by blocking ultraviolet rays irradiated from the sun, and at the same time, free radicals And protects proteins and genes in the skin.

Melanin, which is produced by stress stimuli inside and outside of the skin, is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stress disappears. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results.

In addition, as the number of people enjoying outdoor activities increased due to the increase in the leisure population, the demand for preventing melanin pigmentation due to ultraviolet rays increased.

In response to such demands, substances having ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, derivatives thereof, or tyrosinase inhibiting activity have been conventionally used in cosmetics or pharmaceuticals, The use thereof is limited due to a whitening effect, a safety problem to the skin, a problem of formulation and stability in combination with cosmetics, and the like.

On the other hand, an example of the prior invention of the course tolonaide includes a bone disease treatment and prevention agent containing a course tolonaide (Korean Patent 10-2012-0104869), a blood sugar control, and a magnolia extract having an aldose reductase inhibitory effect (Korean Patent No. 10-2013-0071905), which is contained in the composition of the present invention, and a composition for preventing and treating diabetic complications including an active ingredient (Korean Patent No. 10-2013-0071905). In addition, examples of prior arts related to Laurel water extract include alcohol extracts of Laurel leaves and chloroform fractions (Korean Patent No. 10-2009-0039627), a composition for preventing and treating ischemic nervous system brain diseases, a patent relating to an antioxidant isolated from Laurel leaf and a purification method thereof (Korean Patent No. 10-2000-0007093) In addition, examples of the prior art related to the field of the present invention include a composition for treating renal cancer and a health functional food (Korean Patent No. 10-2011-0040531) There is a patent for a composition for treating brain cancer including a moxibustion extract and a health functional food (Korean Patent No. 10-2011-0005457), but contents having whitening effect on the skin have not been disclosed or taught in any of them.

The object of the present invention is to investigate the effect of inhibiting melanogenesis and improving the pigment deposition by ultraviolet rays through the extraction of a tokenolide derived from a cosolinoid, And an object of the present invention is to provide an excellent cosmetic composition.

The inventors of the present invention have been studying various cosmetics for producing whitening cosmetics having excellent skin whitening effect and excellent stability as a product among the effective substances derived from natural origin, It was confirmed that the extracted substance inhibits melanin biosynthesis and has an excellent effect of improving pigment deposition due to ultraviolet rays, thereby completing the present invention.

In order to achieve the above object, the skin whitening cosmetic composition of the present invention regulates the melanin synthesis pathway to inhibit excessive production of melanin to exhibit skin whitening effect. The skin whitening cosmetic composition according to the present invention contains, as an active ingredient, . ≪ / RTI >

As described above in detail, the present invention provides a cosmetic composition for whitening skin, which comprises, as an active ingredient, a course tonolide contained in a waxy extract and a laurel water extract. The cosmetic composition of the present invention has the effect of inhibiting the melanosomal migration and the clinically whitening effect by using the extract of moss, extract of laurel and the cosolinoid as active ingredients, And can also be used as a cosmetic composition for skin whitening by reducing skin irritation of cosmetics.

Hereinafter, the present invention will be described in more detail.

In general, Laureus nobilis L. is an evergreen tree, native to the Mediterranean coast of southern Europe. It is grown in the southern part of the country as a garden plant, while the northern part of the country is cultivated as a spice plant. Leafy leaves have a bitter taste, but dried leaves are sweet and strong, and they have a unique scent, which is a widely used spice for western cooking. It not only promotes appetite but also improves flavor and buoyancy, and is widely used in cooking such as sauces, sausages, pickles, and fish, meat, and shellfish. Components known as antioxidants include camphene cyanidin, eugenol,? -Terpinene, kaempferol, methyl-eugenol, Myrcene, quercetin and rutin, and in other countries it has been used as neuralgia, rheumatic painkillers, rashes, pustules, extinguishants, astringents, etc. (cf. Choi, YE, 1997)

On the other hand, Saussureae Radix is a roots of Saussurea lappa CLARKE and related plants and contains 0.3-3% of essential oil, and its components are aplotaxene, α- Ionone, β-cellinene, costol and phellandrene. When the ingredients are warm, very tasty and not poisonous, and stopping the diarrhea, (Lee, Yong-Sup, Shin, Min-gyo;

Origin of the field The plant is a perennial herb (Saussurea lappa) belonging to the Asteraceae family. This plant is native to India and is a perennial herb that grows in China. This plant contains various kinds of essential oils, and is used in Oriental medicine for diarrhea, diuretic, genomic, and anthelmintic drugs for gastroenteritis such as diarrhea, vomiting, vomiting and diarrhea. In addition, in the home medicines such as 神 神 丸, Ki-hwan 丸, and 实 毋), in Japan, the directionality is often mixed with the main purpose and consumes a large amount. It is also an important hunting scent. It is the root of sugarcane belonging to Asteraceae and has a peculiar smell and taste. It is used as a main pharmacological treatment for neuropsychiatric treatment, stomach, and duodenal ulcer treatment, helps circulation in the care, and is used as a digestion promoting medicine.

The present invention is such that these pharmacological efficacies are conventionally used for extracting active ingredients of horseradish and laurel, and produced by a method known to a person skilled in the art. For example, water, anhydrous or low-boiling alcohol having 1 to 3 carbon atoms, acetone, ethyl acetate or diethyl ether is added to the pulverized product of the herbal medicine in an amount of 1 to 15 times by volume the dry weight of the pulverized product, By heating at 50 to 100 DEG C for 5 to 24 hours in an extractor equipped with an extruder; Water, an anhydrous or a lower alcohol having 1 to 3 carbon atoms, acetone, ethyl acetate or diethyl ether is added to the pulverized product in an amount of 1 to 15 vol of the dry weight of the pulverized product, Immersing for 3 to 20 days to obtain an extract; An appropriate amount of water was added to anhydrous or hydrous ethanol or methanol extract, and the resulting precipitate was removed by filtration. Ethyl acetate, butanol or diethyl ether was added thereto, followed by well mixing, followed by allowing the layer to separate and separating the upper layer , And the extract is obtained from an extractor equipped with a cooling condenser. These herbal medicine extracts are excellent in safety on the skin. On the other hand, the extraction filtrate of the herbal medicine extract extracted by the above-mentioned method is concentrated under reduced pressure, dried and then added to the cosmetic.

In the cosmetic composition of the present invention, if the content of the extract is less than 0.002% by weight, antioxidative effect and melanin formation inhibitory effect can not be expected in the cell. If the content exceeds 10.0% by weight, There is no increase in the apparent effect and there is a problem in the stability of the shape. The present invention also relates to a skin whitening cosmetic composition characterized in that the whitening cosmetic composition is selected from cosmetics, gels, water-soluble liquids, creams, essences, oil-in-water (O / W) Lt; / RTI >

The whitening combination comprising the course tonolide as an active ingredient can be formulated into a pharmaceutical composition such as a capsule, a liquid preparation, an ointment, a patch or a sustained-release preparation using a pharmaceutically acceptable carrier, and a pharmacologically acceptable base , Starch, tragacanth gum, gelatin, molasses, polyvinyl alcohol, polyvinyl ether, polyvinylpyrrolidone, hydroxypropylcellulose, methylcellulose, ethylcellulose and carboxymethylcellulose) Lubricants such as magnesium stearate, talc, hydrogenated vegetable oil, and the like), disintegrating agents (e.g., agar, starch, bellatin powder, sodium carboxymethyl cellulose, carboxymethylcellulose calcium, crystalline cellulose, calcium carbonate, sodium hydrogencarbonate and sodium alginate) And coloring agents. Examples of the carriers and excipients include lactose, glucose, sucrose, mannitol, potato starch, cornstarch, calcium carbonate, calcium phosphate and cellulose.

In addition to the above, additives such as a stabilizer, a dissolution aid, a peripherial aromatic agent such as percutaneous absorption promoter, and an antiseptic can be further added. The pharmaceutical composition thus prepared can be applied to skin once or several times a day depending on symptoms, The application can be adjusted according to the degree of improvement.

Hereinafter, the present invention will be described in more detail by way of examples. It should be noted, however, that these examples are merely illustrative of the present invention, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of natural extract

After shredding, the shade and laurel were extracted by refluxing three times for 5 hours each in an aqueous 80% (v / v) ethanol solution, followed by cooling and filtration through Whatman # 5 filter paper. The filtered extract is concentrated under reduced pressure and freeze-dried at 50 DEG C or lower to obtain a concentrated extract and a lyophilized product in an amount of 0.001 to 30.0 wt% by using at least one solvent selected from purified water, ethanol, butylene glycol and propylene glycol, Laurel water extract was prepared.

Test Example  One: Intracellular  Effect of active oxygen scavenging

EGCG was used as a comparative sample in order to investigate the effect of inhibiting the formation of active oxygen generated in cells by ultraviolet rays of the horsetail, laurel and course tonolide obtained in Example 1, that is, active oxygen scavenging effect, Was performed.

The cell line used in the experiment was a human keratinocyte HaCaT cell line (Human keratinocytes HaCaT cell line), which was purchased from Dr. Fusenig of the German Cancer Research Center, and it was added to a 96 well black plate for fluorescence measurement dispense 2.0 × 10 ⁴ pieces, and 1 days cultured in penicillin / streptomycin (penicillin / streptomycin) was added DMEM 37 ℃ using (Dulbeccos Modification of Eagles medium, FBS 10%, Gibco, USA) medium, 5% CO ₂ conditions After that, the sample was treated.

After incubation for 24 hours, the cells were washed with HCSS (HEPES-buffered control salt solution) to remove the remaining medium. DCFH-DA (2 ', 7'-dichlorodihydro-fluorescein diacetate, Molecular Probes , USA) was added and incubated at 37 ° C and 5% CO ₂ for 20 minutes, followed by washing with HCSS. The fluorescence intensity of DCF (dichlorofluorescein) oxidized to active oxygen was measured by fluorescence plate reader (Ex (485 nm, Em: 530 nm) after adding 100 μl of HCSS treated at each concentration. After UVB irradiation (20 mJ / cm ² ) was applied, the fluorescence intensity after the sample treatment was measured with a fluorescent plate reader (Ex (485 nm, Em: 530 nm).

As shown in Table 1, the extract of Waxy mildew showed an active oxygen scavenging effect and it was confirmed that the use of co-toluamide resulted in more active oxygen scavenging effect than that of each of them, and EGCG And had an active oxygen scavenging effect.

Name of sample Effect of active oxygen scavenging (%) Wax extract 0.01 wt% 51 Laurel water extract 0.01 wt% 38 Course Tolonide 0.01 wt% 78 EGCG 61

Test Example 2: Comparison of inhibitory effect of tyrosinase activity

In this test example, the effect of inhibiting the tyrosinase activity of the extracts of Lau laet, Laurel water and Cosolanoid in Example 1 was examined. At this time, arbutin, well known as a whitening agent, was used as a comparative sample.

Tyrosinase is an enzyme that stimulates the oxidation of tyrosine in vivo to produce melanin. Tyrosinase was isolated and purified from mushrooms and purchased from Sigma. The substrate L-tyrosine was dissolved in a 0.05 M sodium phosphate buffer solution (pH 6.8) to prepare a 0.1 mg / ml solution. The cosurinolide was dissolved in 0.05 M sodium phosphate buffer solution (pH 6.8) Respectively. 0.5 ml of L-tyrosine solution was added to a test tube, followed by the addition of 0.5 ml of courseinolide, and the mixture was allowed to stand at 37 DEG C for 10 minutes. Thereafter, 0.5 ml of 200 units / ml tyrosinase was added, and the mixture was reacted at 37 占 폚 for 10 minutes. The reaction tube was placed on ice to quench the reaction, and the absorbance at 475 nm was measured by a spectrophotometer. As a control group, 0.5 ml of a buffer solution was used instead of a course of anolyte. At this time, the inhibitory activity (%) against tyrosinase was calculated by the following equation (1), and the IC ₅₀ value when the tyrosinase activity was inhibited by 50% was measured for each whitening substance. The results are shown in Table 2 below.

Whitening substance IC ₅₀ (ug / ml) (concentration required for 50% inhibition of tyrosinase activity) Wax extract 210.90 Laurel water extract 321.62 Course Tonight 196.11 Arbutin 250.52

As a result of the experiment, it was considered that the concentration of the course tonolide inhibiting 50% of the tyrosinase activity rate was 196.11 ug / ml, 210.90 ug / ml of the woody extract, 321.62 ug / ml of the laurel water extract and 250.52 ug / ml of the arbutin , It was confirmed that the effect of inhibiting the tyrosinase activity of the cosolanoid and the moss extract was excellent.

Test Example 3: Measurement of inhibitory effect on melanin formation

The melanin synthesis inhibitory effect on B16F0 melanocytes was measured in order to confirm the whitening effect of the hops, laurel water, and course tolunide obtained in Example 1. The inhibitory effect of B16F0 melanin cells on melanin synthesis was measured as follows. B16F0 melanocytes were plated at a concentration of 2 x 10 ⁶ per well in a 6-well plate and the cells were treated with a concentration that did not cause toxicity after 72 hours of incubation. After incubation for 72 hours, the cells were detached with trypsin-EDTA, and the number of cells was measured, followed by centrifugation to recover the cells. Quantification of intracellular melanin was carried out with a slight modification of the method of Lotan ( Cancer Res. , 40: 3345-3350, 1980). The cell pellet was washed once with PBS, and 1 ml of homogenization buffer (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt the cells. After centrifugation (3,000 rpm, 10 min), 1N NaOH (10% DMSO) was added to the cell filtrate to dissolve the extracted melanin, and the absorbance of melanin was measured at 405 nm with a microplate reader. Melanin was quantitated to determine the melanin The percent inhibition of production was measured. The inhibition rate (%) of melanin production of B16F0 melanocytes was calculated by the following equation (2).

A: Amount of melanin in wells to which no sample was added

B: Amount of melanin in the well to which the sample was added

Name of sample Melanin inhibition rate (%) Wax extract 0.25 wt% 81.46 Laurel water extract 0.25 wt% 77.81 Course Tolonide 0.25 wt% 91.49 Arbutin 0.25% by weight 57.88

B16F0 melanin cells were inhibited by melanin formation. Melanin production inhibitory effect was shown by the extracts of Mackerel and Laurel. Melatonin production inhibitory effect was also confirmed by using of courseinolide. (Table 3). The results are shown in Table 3.

Comparative Example 1: Whitening cosmetic composition containing arbutin

This preparation was intended to produce a whitening cosmetic composition containing arbutin.

The whitening cosmetic composition of the essence formulation was prepared as shown in Table 4 below.

(Unit: parts by weight) ingredient Comparative Example Manufacturing example Course Tonight - 2.0 Arbutin 2.0 - glycerin 10.0 10.0 Betaine 5.0 5.0 PEG 1600 2.0 2.0 EDTA-2Na 0.02 0.02 Hydroxyethylcellulose 0.1 0.1 Sodium hyaruronate 5.0 5.0 Carboxyvinyl polymer 0.2 0.2 TEA 0.15 0.15 Octyldodecanol 0.3 0.3 ethanol 6.0 6.0 incense 0.2 0.2 Germall 116 0.3 0.3 Purified water qs to 100 qs to 100

Test Example  4: Skin whitening Cosmetics  Analysis of whitening efficacy of the composition

In this test example, the whitening effect of the skin whitening cosmetic composition containing the course tonolide of Production Example 1 as an active ingredient and the skin whitening cosmetic composition containing arbutin as an active ingredient of Comparative Example 1 was examined.

In order to test the clinical whitening effect of the whitening cosmetic composition of the present invention, 40 healthy adult women with pheochromocytosis were randomly divided into two groups (A and B) by 20 persons, 1, and the whitening cosmetic composition of Comparative Example 1 was applied to the B group for 2 weeks, twice a day for 8 weeks. Subsequently, the change in skin color was measured by using a spectrophotometer (Minolta CM600-d) to measure the change in brightness (DELTA L) of the color. Objective visual observation with a plurality of experts and subjective visual observation with the subject were performed to obtain a whitening effect Respectively. The results are shown in Table 5 below. At this time, the degree of whitening efficacy was evaluated by the following seven grades.

<Whitening efficacy evaluation standard>

-3: very bad -2: worse -1: slightly worse 0: no change

1: Slight improvement 2: Slight improvement 3: Slight improvement

Subject Change in skin color intensity Objective evaluation of expert Subjective evaluation of the subject Group A Group B Group A Group B Group A Group B One 3.2 1.8 2 One 2 One 2 2.6 1.9 0 One One One 3 2.7 1.6 One 0 One 0 4 3.1 1.7 2 0 One 0 5 3.6 2.0 2 One 2 One 6 3.4 2.0 One One One One 7 4.0 2.2 2 One 2 One 8 3.5 1.7 One 0 One 0 9 2.9 1.2 One 0 0 0 10 3.1 1.5 One 0 One 0 11 4.2 2.4 2 One 2 One 12 2.7 2.0 One One 0 One 13 3.5 2.1 2 One One One 14 3.7 1.7 2 0 2 0 15 3.1 1.8 One 0 One 0 16 4.3 2.6 2 2 2 2 17 2.1 1.5 0 0 0 0 18 3.8 2.6 2 One One 0 19 3.4 2.8 One One 0 One 20 3.6 2.8 One 0 One 2 Average 3.33 2.00 1.35 0.60 1.10 0.65

As a result of the experiment, the average L value of the group A to which the whitening cosmetic composition containing the cosolinoid of the present invention was applied was 3.33 (p < 0.01), and the average L value of the B group to which the whitening cosmetic composition containing arbutin was applied (Group A) and group B (group B) were 2.00 (p <0.05) and 1.15 for group A and 0.60 for group B, respectively.

From the above results, it was confirmed that the whitening cosmetic composition containing the course tonolide of the present invention is superior in skin whitening efficacy to the existing whitening cosmetic composition containing arbutin.

Experimental Example 5: Stability test of formulation

This Experimental Example was intended to examine the formulation stability of the skin whitening cosmetic composition containing the course tonolide of Production Example 1 as an active ingredient and the skin whitening cosmetic composition containing arbutin as an active ingredient of Comparative Example 1.

The skin whitening cosmetic composition containing the course tonolide of Preparation Example 1 as an active ingredient and the skin whitening cosmetic composition containing arbutin as an active ingredient of Comparative Example 1 were placed in a thermostat kept constant at 45 캜, And then stored for 2 weeks in a glass container and kept in a completely shaded refrigerator maintained at 4 ° C in an opaque glass container for 2 weeks.

The test results are shown in Table 6 below. At this time, the degree of discoloration of the product was classified into the following six grades and evaluated.

&Lt; Evaluation criteria of product discoloration >

0: No change 1: Extremely slightly changed 2: Slightly changed 3: Slightly changed

4: Severely changed 5: Extremely severe

Temperature Degree of discoloration of test substance Production Example 1 Comparative Example 1 45 ° C 0.8 4.2 4 ℃ 0 1.1

As a result of the experiment, it was found that the whitening cosmetic composition containing the course tonolide of the present invention hardly discolored and thus excellent in stability as a formulation.

Test Example 6: Skin irritation effect test

In order to test skin irritation of each cosmetic manufactured in Table 4, patch test was performed on 20 healthy male and female adults. (Finn chamber, 100 x 10, EPITEST, Finland) containing the samples of Example 1 and Comparative Example 1 was applied to the inner part under the arm, and after 24 hours and 48 hours, the patches were removed and the magnifying glass 8MC- 150, DAZOR, USA), and the average of 20 subjects was calculated according to the criteria of the International Society for Contact Dermatology Study. The results are shown in Table 7 below.

(Evaluation criteria and score of skin reaction)

6: Formation of algebras

3: Severe erythema and vesicle formation

2: Severe erythema, papules and small follicles

1: The border is clear but weak erythema, edema and palsy

0.5: faint or mild erythema

0: no response, normal skin

division Example 1 Comparative Example 8 24 hours 0.33 0.19 48 hours 0.09 0.08

As shown in Table 6, it was confirmed that the degree of skin irritation in Example 1 containing the course tonolide used in the present invention was similar to that in the case of using arbutin as in Comparative Example 1, without skin irritation.

Claims (4)

A cosmetic composition for skin whitening comprising an extract of moss, laurel extract and a cosolide as an active ingredient. [Claim 2] The method according to claim 1, wherein the laurel extract and wort extract are selected from the group consisting of water, an anhydrous or a lower alcohol having 1-6 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol Wherein the cosmetic composition is a cosmetic composition for skin whitening. The cosmetic composition for skin whitening according to claim 1, wherein the cosmetic composition comprises 0.001-10.0% by weight based on the total weight of the cosmetic composition containing the laurel wax extract, wax extract, and the cosuronolide. The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray &Lt; / RTI &gt; wherein the composition has a formulation selected from the group consisting of: &lt; RTI ID = 0.0 &gt;