KR102030419B1 - A composition comprising graviola having inhibitory effect on induction of grey hair and positive effect on induction of black hair - Google Patents

Abstract

The present invention relates to a composition for preventing gray hair and promoting hair growth. The composition for preventing gray hair and promoting black hair according to the present invention may be one containing a gravola extract.
In the case of the composition for preventing gray hair and promoting hair growth, it is possible to prevent white hair and promote the production of black hair by using a natural composition that exhibits an scavenging ability for H ₂ O ₂ DPPH radicals and a reducing power for active oxygen.
In particular, the composition for the prevention of gray hair and the promotion of black hair induction can provide a significant effect on the prevention of gray hair and promote black hair induction without providing any side effects of skin or hair damage as the extract composition derived from natural products as a composite composition.

Description

A composition for preventing gray hair and promoting hair loss, including gravola {A COMPOSITION COMPRISING GRAVIOLA HAVING INHIBITORY EFFECT ON INDUCTION OF GRAY HAIR AND POSITIVE EFFECT ON INDUCTION OF BLACK HAIR}

The present invention relates to a composition for preventing gray hair and promoting hair growth, including gravola. More specifically, by utilizing a natural composition exhibiting scavenging ability against H ₂ O ₂ DPPH radicals and reducing ability against free radicals, it promotes melanin synthesis by reducing H ₂ O ₂ accumulated around hair follicles. It is to provide a composition for preventing gray hair and promoting black hair induction to prevent, and induce black hair.

Hair is composed of three parts: cuticle, fur, and medulla. The cortex that surrounds the medulla is the thickest part of the hair (80 to 90%), the melanin and lipid membranes called melanosomes, tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2) and It contains granules composed of other proteins. Melanin pigment is synthesized in the melanocytes, which are organelles in melanocytes, and migrates to the surrounding keratinocytes through the resinous lumen of melanocytes. In the synthesis route of melanin, the tyrosinase is involved in the first step in which the amino acid tyrosine is oxidized, the doparo is oxidized, and the dopaquinone is produced, and the reaction is automatically oxidized, but the TRP-1 and TRP-2 Enzymes such as these are known to accelerate the reaction. The color of the hair determines the brightness of the hair by factors such as the type and the amount of distribution of eumelanin and pheomelanin formed from the amino acid tyrosine as a raw material, thereby revealing a unique hair color for each person. In other words, it can be determined that various hair colors such as black, red, and blonde are determined according to the type of melanin and the size of granules. The hair color is the two most common melanin-colored granules in the cortex, the hair's middle layer, the most common and darkest pigments that produce brown and black hair. Pheomelanin is a lighter color tone that gives blond hair. The color of the red hair is represented by the red and black pigments, and the blond hair is the result of a mixture of red and yellow pigments. Sandy brown hair is pigmented by a mixture of red, brown, and black pigments, while dark brown hair contains more black pigments than sandy brown hair. And gray hair does not have pigment granules. Hair color is determined by the pigments present in the hair, and these pigments are determined by their genetic information factors.

Melanin is a protein that does not dissolve in water and gives color to hair. Tyrosine, an amino acid produced in breast nipple, is the substrate of melanin. White hair stops its action in dopaquinone, so hair is keratinized and rises over keratin, and white hair is not oxidized because it does not produce tyrosanase enzymes. White hair is particularly evident in races with dark brown hair and is a type of aging that occurs because melanin production stops in melanocytes.

Dopaquinone proceeds in two ways. Through dopachrome, there are pathways to produce dark brown eumelanin, and dopaquinone is combined with cysteine present in keratin protein to produce reddish brown pheomelanin. As such, the oxidative dyeing of the hair is a result of the polymerization reaction in the hair. In this reaction, aromatic amines and phenolmonomers are oxidatively polymerized and bind to amino acid residues in the hair. Eumelanin is relatively large in size and can be easily destroyed chemically, whereas femelanin is relatively small in size and has a chemically stable structure. The high cysteine hair contains a lot of pheomelanin, and when the brown hair is decolorized with hydrogen peroxide, the color appears slightly red because the eumelanin is first destroyed and the pheomelanin remains.

Melanin in hair is an important factor in determining the color tone of hair, and is transferred to hair cortical cell keratinocytes generated in melanocytes present in the upper part of hair, and then moved upward with hair growth. White hair, which is recognized as a phenomenon of physiological aging, is caused by a decrease in the number of hair melanocytes and a decrease in melanin production due to a decrease in melanocyte function.

White hair generally occurs when the age increases, deepening the impression of old age, and especially hate gray hair at a young age. Therefore, in order to hide gray hair, many dyes which blacken outbreaks by white hair dye or the like have been studied and widely marketed. However, black hair dyeing must be dyed again by the growth of white hair in a temporary manner, and oxidative black hair dyeing, which is currently widely used, damages the skin by use of an oxidizing agent or the like. Therefore, various studies have been conducted on the anti-white hair preventive agent which prevents the occurrence of gray hair or the black hair promoter which induces the production of black hair.

However, side effects such as damage to the hair or skin when using the composite is problematic, there is a problem that the usage is limited by its use due to cytotoxicity, etc. For natural products, compositions derived from natural products known to date cause gray hair suppression and black hair There is a limit that the effect is relatively small.

Therefore, by using a composition derived from a variety of natural ingredients to solve the problems of side effects such as hair or skin damage caused by the use of the composition while preventing the white hair and to promote the production of black hair more effectively, the composition for the prevention of gray hair and black hair induction Development and excavation are urgently needed.

In related prior art, KR 10-2002-0059906 A (Prior Art 1, Publication Date: July 16, 2002) combines Sukjihwang, Astronomical and Macmundong as supplementary ingredients in black sesame, rosacea, nasturtium and honey. The method of use is disclosed. In addition, KR 10-2002-0059905 A (prior art 2, published date: July 16, 2002) is also available in a container with black soybean, along with red sesame, cypress leaf, sperm, nasturtium, stone salt and succinate yellow, astronomical dong and mackmundong. It is boiled at a temperature of 50 ℃ after the addition and the method discloses a method in which the medicinal solution to maintain the black soybean, Japanese Patent Publication JP 56-087515 A (prior art 3, publication date: July 16, 1981) is black sesame, black pine leaves , A method using kelp is disclosed. In addition, Japanese Laid-Open Publication No. JP 60-178805 A (prior art 4, publication date: September 12, 1985) discloses a method of using a plant extract using an extract of Ogalpi as an active ingredient. In addition, using pituitary hormone (Japanese Patent Publication No. 62-63510), C-AMP (Adenosine-3,5 'Cyclic Phosphate) (Japanese Patent Publication No. 62-45527), or using Vitamin D3 (Japanese Patent Publication No. 60-174705) and the like are known.

KR 10-2002-0059906 A KR 10-2002-0059905 A JP 56-087515 A JP 60-178805 A

It is an object of the present invention to prevent gray hair by using a natural composition exhibiting the scavenging ability to H ₂ O ₂ DPPH radicals and reducing power to free radicals, and to prevent gray hair and gray hair, including a gravure to promote the production of black hair. It is to provide a composition for promoting induction.

In particular, by utilizing the extract composition derived from natural products to provide a composite composition that can have a significant effect on the prevention of gray hair and the promotion of black hair induction without the side effects of skin or hair damage.

Another object of the present invention is to provide a gravureola extract composition to a certain range to prevent gray hair including gravola, and to promote hair follicles, while preventing the gray hair including gravola, which can be used as an external preparation for hair through excellent flavor It is to provide a hair external preparation for the prevention of gray hair and black hair induction, including gravureola prepared using the composition for promotion.

Still another object of the present invention is to provide a method for preparing a composition for preventing gray hair and promoting black hair, including the gravola.

In order to achieve the above object, the composition for preventing gray hair and promoting black hair in accordance with an embodiment of the present invention may be to include a gravola extract.

The composition for preventing gray hair and promoting hair growth may further include black sesame extract and black ginseng extract.

The composition for preventing gray hair and promoting black hair may be further comprising black rice extract and brown rice extract.

The composition for preventing gray hair and promoting hair growth may further include black sesame extract, black ginseng extract, black rice extract, brown rice extract, and Krachaidam extract.

In the composition for preventing gray hair and promoting hair growth, the gravure extract, black sesame extract, black ginseng extract, black rice extract, brown rice extract, and kratchaidam extract may be extracted with 10 to 99% by weight of methanol or ethanol as a solvent. .

According to another embodiment of the present invention, the food composition for preventing gray hair and promoting black hair induction may include a composition for preventing gray hair and promoting black hair.

Hair external composition for the prevention of gray hair and black hair induction according to another embodiment of the present invention may include a composition for preventing gray hair and promoting black hair induction.

According to another embodiment of the present invention, the hair cleaning composition for preventing gray hair and promoting hair growth may include a composition for preventing gray hair and promoting hair growth.

According to another embodiment of the present invention, a method for preparing a composition for preventing gray hair and promoting black hair induction is carried out by drying and crushing gravure, and then preparing 10 to 99% by weight of methanol or ethanol solvent and gravure powder. 1 to 1: 1 part by weight to prepare a gravure extract gravure extract gravola extract; Drying the black sediment, pulverizing and then mixing the 10 to 99% by weight of methanol or ethanol solvent and the black sediment into 10: 1 to 1: 1 parts by weight to produce a black sesame extract; Black ginseng extract manufacturing step of preparing black ginseng extract by drying black ginseng, pulverizing and mixing 10 to 99% by weight of methanol or ethanol solvent and the black ginseng pulverized product in 10: 1 to 1: 1 parts by weight; Drying the black rice, pulverizing, and then preparing a black rice extract by mixing 10 to 99 wt% of methanol or ethanol solvent with the black rice crushed product in an amount of 10: 1 to 1: 1 by weight to prepare a black rice extract; A brown rice extract preparing step of preparing brown rice extract by drying brown rice, pulverizing and mixing 10 to 99 wt% of methanol or tanol solvent with 10 to 1 to 1 part by weight of the brown rice mill; Drying, grinding, and then to break up the Krai Chai Dam 10 to 99% by weight of methanol or ethanol solvent and the Krai Chai Dam crushed to 10: 1 to 1: 1 parts by weight to prepare a Krai Chai Da extract extract step; And, it may include a step of mixing the gravola extract, black sesame extract, black ginseng extract, black rice extract, brown rice extract and Kla chidam extract.

Hereinafter, the present invention will be described in more detail.

The composition for preventing gray hair and promoting black hair in accordance with an embodiment of the present invention may include a gravureola (Graviola) extract.

Since the gravureola (Graviola) extract has the effect of promoting the melanin production with the antioxidant effect, it can promote gray hair prevention and black hair induction when the gravureola (Graviola) extract.

To return white hair back to black hair, melanocytes in the hair follicles must produce melanin pigment. In addition, melanocytes in the basal layer of the skin synthesize melanin as a protective mechanism to protect the skin from harmful stimuli such as ultraviolet light.

In the melanin synthesis mechanism, tyrosinase enzyme is an important catalytic enzyme that determines the initial rate of melanin synthesis. To synthesize melanin, tyrosinase combines hydroxyl functional groups with tyrosine to produce 3,4-dihydroxy phenylalanine (DOPA), which simultaneously oxidizes DOPA and converts it into dopaquinon. The next step, oxidized dopaquinon, is converted to 5,6-Dihydroxy-2-indolycarboxylic acid (DHICA) by the second enzyme tyrosinase related protein-2 (TRP-2). This DHICA is converted to indole-5,6-quinone caboxylic acid by a third enzyme, tyrosinase related protein-1 (TRP-1), which is oxidized to produce eumelanin.

Another melanin synthesis mechanism is the synthesis of melanin by oxidation of unstable reactive oxygen species (ROS) such as hydrogen peroxide, superoxide ion and hydroxyl radical. Therefore, melanin production is promoted by melanocytes in hair follicle cells, which can turn white hair into black hair. Recently, α-melanocyte stimulating hormone (α-MSH), imatinib mesylate and 3-isobutyl-1-methylxanthine (IBMX) are known as substances which increase the production of Melanin. α-MSH amplifies cAMP, an important messenger for skin pigmentation regulation, and is activated by protein kinase A (PKA). Protein kinase A (PKA) is activated. Thus, it regulates tyrosinase activity, which affects the proliferation and pigmentation of melanin cells.

Imatinib mesylate is a tyrosine kinase inhibitor known to cure chronic myeloid leukemia (CML) caused by tyrosine kinase. Imatinib mesylate also inhibits c-kit tyrosinase kinase and is involved in the survival and development of melanin cells of c-kit and its ligand stem cell factor. IBMX inhibits cAMP phosphodiesterase and activates PKA by increasing the cAMP content in cells. PKA can phosphorylate CREB, a transcription factor, and upregulate tyrosinase and MITF, key enzymes in the process of promoting melanin production.

In addition, as the main cause of the change of the black hair to white hair, H ₂ O ₂ accumulates around the hair follicles, causing melanin (melanin) synthesis to deteriorate, causing white hair. Therefore, the composition for preventing gray hair and promoting black hair has a scavenging ability for H ₂ O ₂ DPPH radicals and a reducing power for free radicals. Thus, when using the composition for preventing gray hair and promoting black hair, H ₂ O is an active oxygen. The reduction of ₂ may promote the synthesis of melanin to prevent white hair and promote the formation of black hair.

The composition for preventing gray hair and promoting hair growth may further include black sesame extract and black ginseng extract.

The black sesame is a term for the black sesame seeds in one shot.

The black ginseng means hyeonsam (Scrophularia root).

When the gravureola extract further comprises the black sesame extract and black ginseng extract, the effect of promoting hair follicles may be increased, thereby resulting in a high effect with less usage. Therefore, it is possible to use a lower dose based on the effect has the advantage of keeping the cytotoxicity low.

Preferably, the composition for preventing gray hair and promoting black hair, including the gravola, includes gravola extract, and includes 10-1000 parts by weight of the black sesame extract and 10-1000 parts by weight of black ginseng extract based on 100 parts by weight of the gravola extract. In the case of the above range, the effect of promoting black hair induction by mixed use may be improved.

More preferably, the composition for preventing gray hair and promoting black hair, including the gravola, includes gravola extract, and 200 to 400 parts by weight of the black sesame extract and 100 to 200 parts by weight of black ginseng extract based on 100 parts by weight of the gravola extract. According to the above range, as a combination of the use of the black sesame extract and black ginseng extract to increase the hair follicle-promoting effect is significantly higher and the cytotoxicity is lowered, as a composition for preventing gray hair and promoting hair loss without adverse effects. Has the advantage to utilize.

The composition for preventing gray hair and promoting black hair may be further comprising black rice extract and brown rice extract.

The black rice refers to black rice.

The brown rice refers to rice which has been chaffed with a machine made of a rubber roller after drying and threshing the harvested rice.

When the gravure extract further includes the black rice extract and brown rice extract, the scavenging ability and H ₂ O ₂ DPPH radical can be increased as well as the reducing power for free radicals, and the melanin-producing effect can be further promoted by promoting melanin production. have.

Preferably, the composition for preventing gray hair and promoting black hair, including the gravola, comprises gravola extract, and includes 10 to 1000 parts by weight of the black rice extract and 10 to 1000 parts by weight of brown rice extract based on 100 parts by weight of the gravola extract. In the case of the above range, the effect of promoting black hair induction by mixed use may be improved.

More preferably, the composition for preventing gray hair and promoting black hair, including the gravola, includes gravola extract, and 50 to 200 parts by weight of the black rice extract and 50 to 200 parts by weight of brown rice extract based on 100 parts by weight of the gravola extract. There is something to include,

According to the above range, since the scavenging ability to H ₂ O ₂ DPPH radical and the reducing power to free radicals are greatly improved, and cytotoxicity is lowered, even when used in a small amount, it can have a high effect on promoting the appearance of black hair.

The composition for preventing gray hair and promoting hair growth may further include black sesame extract, black ginseng extract, black rice extract, brown rice extract, and Krachaidam extract.

When gravureola extract, black sesame extract, black ginseng extract, black rice extract, brown rice extract and kratchaidam extract are used, the active ingredient that promotes TRP-2 expression, an enzyme involved in the process of producing melanin, increases, and H ₂ As the scavenging ability of O ₂ DPPH radical increases, it can have a higher effect of causing black hair.

Preferably, the composition for preventing gray hair and promoting black hair, including the gravola, includes gravola extract, and 200 to 400 parts by weight of black sesame extract based on 100 parts by weight of the gravure extract to 100 to 200 parts by weight of the black ginseng extract; 50 to 200 parts by weight of the black rice extract; 50 to 200 parts by weight of the brown rice extract and 10 to 50 parts by weight of the brachydam extract may be included.

When used in a mixed range, the scavenging ability of H ₂ O ₂ DPPH radicals is greatly increased, and the melanin synthesis may be promoted to significantly increase the hair follicle-inducing effect.

In addition, more preferably, the composition for preventing gray hair and promoting black hair including the gravola comprises a gravola extract, and the black sesame extract 250 to 350 parts by weight based on 100 parts by weight of the gravola extract extract 130 to 180 Parts by weight; 90 to 150 parts by weight of the black rice extract; 90 to 150 parts by weight of the brown rice extract and 20 to 40 parts by weight of the Krathaydam extract may be included.

According to the above range, H ₂ O ₂ can effectively remove H ₂ O ₂ accumulated in hair follicles by eliminating H ₂ O ₂ DPPH radicals and high reducing power to active corals, and are an enzyme involved in the process of producing melanin. The effect of promoting melanin synthesis by promoting TRP-2 expression is most prominent.

Meanwhile, preferably, the composition for preventing gray hair and promoting black hair induction including the gravola may further include a banana extract.

Bananas, grown in wet lowlands in tropical and subtropical regions, are one of the oldest representative wild fruits on earth, along with human history. Bananas are rich in nutrients such as calcium, carotene, and vitamin A, and they are among the highest in calories.

Bananas consist of a peel and the flesh inside, but it is generally used to refer to the flesh used for food. However, 'banana' used throughout the specification of the present invention is not limited to banana pulp, and is defined as a meaning including both banana pulp and its peel. Therefore, banana extract is defined as belonging to the upper concept for banana pulp extract and banana peel extract.

Banana extract promotes melanin synthesis as well as cell activity of melanocytes to prevent white hair and promote the induction of black hair.

Preferably the banana extract may be a banana peel extract.

Banana peel extract was confirmed that the effect of causing black hair is significantly higher than the banana pulp extract. Banana peel contains tyrosine, tryptophan, iron, potassium, B vitamins, vitamins C and K, magnesium, fiber, antioxidants, and copper, which are precursors of melanin synthesis, and when oxidized, the tyrosine contained in bananas is converted to melanin It is believed that the banana peel extract plays a role of significantly increasing the effect of promoting the appearance of black hair.

In addition, the banana extract is excellent in promoting the prevention of gray hair and hair follicles, and there is almost no problem such as side effects due to low cell rust. In addition, it has the advantage that it can be utilized as a functional product that has excellent flavor and provides a composition containing a banana extract in a variety of formulations to promote palate defense and black hair induction effect.

That is, the composition for the prevention of gray hair and black hair induction including the banana is provided as a shampoo, conditioner, hair gel, ointment, cream, lotion, lotion, spray, ampoule, etc., to prevent white hair in advance, and to generate black hair. It can be usefully used as a hair cosmetic for promoting hair aging.

In addition, since the banana extract itself is very excellent in the removal effect of H ₂ O _{2 and} the effect of promoting excellent melanin synthesis is considerable, when used in combination with the gravola can greatly increase the effect of preventing black hair and white hair.

In the composition for preventing gray hair and promoting black hair, the gravure extract, black sesame extract, black ginseng extract, black rice extract, brown rice extract and Kratchaidam extract may be extracted with 10 to 99% by weight of methanol or ethanol as a solvent. have.

When the methanol or ethanol content of the solvent is less than 10% by weight, almost no active ingredient promoting melanin production is obtained. When the content of methanol or ethanol exceeds 99% by weight, the cytotoxic component of the extract is extracted and the composition is extracted. There is a limitation in the amount of the use, and the melanin synthesis effect is inhibited by the extraction of ingredients other than the active ingredient.

Preferably the solvent may be 75 to 95% by weight of methanol or ethanol extract. When ethanol in the above range is used as a solvent, it may be advantageous to promote melanin production and to obtain an effective ingredient capable of effectively removing H ₂ O ₂ accumulated in hair follicles.

Most preferably, the gravure extract is using methanol as a solvent, and the composition of other plant extracts may be ethanol as a solvent.

The food composition referred to in the present invention is defined as meaning food, drink or the like that can be ingested due to functionality or palatability.

Preferably, the food composition for preventing gray hair and promoting hair follicles comprising the gravola is 11 to 20 parts by weight of the cherry extract, and the thorn tree extracts 50 to 100 parts by weight of the composition for preventing gray hair and the hair follicle promotion comprising gravure. 80 parts by weight, may be further comprising 1 to 5 parts by weight of rooibos extract.

In the case of including the cherry extract, thorn tree extract and rooibos extract in the above range may be in harmony with the sweet and subtle aroma of the gravure extract as a whole, deep flavor, especially black sesame extract, black ginseng extract, black rice extract, brown rice extract and Even if Krapchaidam extract is used together with Graviola extracts, the bitter flavor of black ginseng and the strong flavor of Kratchaidam are removed in accordance with the above range, and the sweet, fragrant and deep aroma is harmonized as a food composition with high taste. Can be provided.

When the cherry extract contains less than 11 parts by weight, the sweet and light flavor of the gravure extract and the cloudy flavor of the cherries will have a flat flavor as a whole, when provided as a complex extract containing gravola extract extract bitter flavor of black ginseng extract is removed There is no problem. In addition, if the amount exceeds 20 parts by weight of the cherry and the strong taste of cherries do not blend well with the sweetness and its aroma of gravola, there is a problem that the overall flavor can not be harmonized and palatability is lowered.

The hemberry extract is not effective in removing the strong aroma of the Krai Chaidam when mixed in less than 50 weight ratio. In addition, there is a problem that the aroma of black ginseng is not removed even when provided as a complex extract containing a gravola extract. In addition, there is a problem that the hummus tree extract exceeds 80 parts by weight as a whole, the mingham taste is strong and palatability is lowered.

If rooibos extract is used, it improves palatability by neutralizing bitter taste of black ginseng and the scent of Kratchaidam, while enhancing the overall flavor. However, when the rooibos extract is used in less than 1 part by weight, the overall effect of neutralizing the bitter taste of the black ginseng and the scent of the Kratchaidam when used as a complex extract containing the gravure extract as a whole with gravure ola. Can't make it. In addition, in the case of exceeding 5 parts by weight, the scent of the rooibos itself becomes stronger, there is no flavor of other raw materials, there is a problem that the palatability of the overall flavor is inferior to the palatability.

Therefore, it may be advantageous to provide a functional food that can prevent gray hair and promote black hair induction while having high palatability according to the above range.

The cherries extract, thorn tree extract and rooibos extract may be using the ethanol extract. When the ethanol extract is used, it may be advantageous to mix with the composition for preventing gray hair and promoting hair growth.

Hair external composition for gray hair prevention and black hair induction promoting gravure comprising a gravureola according to another embodiment of the present invention may be to include the composition for the prevention of gray hair and promote hair growth.

The topical hair composition according to the present invention refers to a topical composition which is provided in a form such as to be sprayed or applied to the hair for functional or aesthetic properties, and includes a hair essence, a hair nutrient solution, a hair cream, a hair lotion, a hair ointment, a hair gel, This includes hair sprays, and is not limited to the products listed above.

Gray hair prevention and black hair induction promoting hair cleaning composition comprising a gravureola according to another embodiment of the present invention may be to include the composition for the prevention and gray hair induction.

The hair cleaning composition used in the present invention refers to a composition used for the purpose of washing or washing hair, and the like, including shampoo, rinse, hair soap, hair cleaner, and the like, but is not limited to the above-listed products.

Preferably, the composition for external application for hair and the composition for washing hair including the gravureola are 1 to 10 parts by weight of dandelion, 1 to 5 parts by weight of rooibos and 5 to 10 parts by weight of grape seed extract, based on 100 parts by weight of the composition for preventing hair loss and promoting black hair. It may be to include more parts by weight.

When the dandelion extract, rooibos extract and grape seed extract is included to protect the scalp and hair to improve the scalp and hair health, as well as having an excellent fragrance divergence effect using excellent natural products.

When the dandelion extract is included in less than 1 part by weight, there is a problem in that the effect of enhancing the fragrance of the external preparation composition or the cleaning composition is not only insignificant, but also the scalp protection effect is low. In addition, if more than 10 parts by weight of the strong dandelion fragrance is not well matched, the content of the dandelion extract is relatively increased, there is a problem that the scalp and hair improvement effect is rather reduced.

When the rooibos extract is used in the above range, it may have an effect of removing the fragrance of the Kratchaidam.

When the grape seed extract is used in less than 5 parts by weight, there is a problem that the scalp and hair improvement effect is lowered, and when it exceeds 10 parts by weight, the oil content is increased and the feeling is not good, the scalp cleaning effect is lowered and the gray hair prevention is prevented. And it may interfere with the removal of H ₂ O ₂ DPPH radical of the hair follicle by the composition for promoting hair growth.

Therefore, according to the above range can be advantageous to provide a functional hair external preparation or a functional hair cleaning composition having excellent fragrance while preventing gray hair and promoting black hair induction, as well as high scalp and hair improvement effect.

The dandelion extract, rooibos extract and grape seed extract may be using an ethanol extract. When the ethanol extract is used, it may be advantageous to mix with the composition for preventing gray hair and promoting hair growth.

According to another embodiment of the present invention, a method for preparing a composition for preventing gray hair and promoting hair follicles may be performed by drying and crushing gravola, and then preparing 10 to 99% by weight of ethanol solvent and the gravure crushed powder. 1: 1 to 1 part by weight of the gravureola extract manufacturing step to produce a gravure extract; Drying the black sediment, pulverizing and then mixing the 10 to 99% by weight of methanol or ethanol solvent and the black sediment into 10: 1 to 1: 1 parts by weight to produce a black sesame extract; Black ginseng extract manufacturing step of preparing black ginseng extract by drying black ginseng, pulverizing and mixing 10 to 99% by weight of methanol or ethanol solvent and the black ginseng pulverized product in 10: 1 to 1: 1 parts by weight; Drying the black rice, pulverizing, and then preparing a black rice extract by mixing 10 to 99 wt% of methanol or ethanol solvent with the black rice crushed product in an amount of 10: 1 to 1: 1 by weight to prepare a black rice extract; Drying brown rice, pulverizing, and then preparing brown rice extract by mixing 10 to 99% by weight of methanol or ethanol solvent with the brown rice pulverized product in an amount of 10: 1 to 1: 1 by weight; Drying, grinding, and then to break up the Krai Chai Dam 10 to 99% by weight of methanol or ethanol solvent and the Krai Chai Dam crushed to 10: 1 to 1: 1 parts by weight to prepare a Krai Chai Da extract extract step; And, it may include a step of mixing the gravola extract, black sesame extract, black ginseng extract, black rice extract, brown rice extract and Kla chidam extract.

The composition for preventing gray hair and promoting hair growth according to the present invention includes a gravure extract, and utilizes a natural composition exhibiting scavenging ability for H ₂ O ₂ DPPH radicals and reducing power for free radicals to prevent gray hair and produce black hair. Can promote.

In particular, the composition for the prevention of gray hair and the promotion of black hair induction can provide a significant effect on the prevention of gray hair and promote black hair induction without providing any side effects of skin or hair damage as the extract composition derived from natural products as a composite composition.

In addition, the hair external preparation for gray hair prevention and black hair induction promoting according to the present invention can be used as a hair external preparation through the excellent flavor with gravureola extract to include a range of white hair prevention and black hair induction. And it can be prepared using the composition for the prevention of gray hair and black hair induction.

In addition, the present invention provides a method for preparing the composition for preventing gray hair and promoting hair growth.

1 relates to a DPPH radical scavenging assay test results for extracts according to an embodiment of the present invention.
Figure 2 relates to the active oxygen removal effect test for the extract according to an embodiment of the present invention.
Figure 3 relates to the results of in vitro tyrosinase activity measurement for the extract according to an embodiment of the present invention.
Figure 4 relates to the results of in vitro melanin synthesis measurement for the extract according to an embodiment of the present invention.
Figure 5 relates to the cytotoxicity test results for the extract according to an embodiment of the present invention.
Figure 6 relates to the measurement results of Melanin production in B16F1 for the extract according to an embodiment of the present invention.
Figure 7 is compared with the results of comparative experiments of the degree of in vitro melanin synthesis for the extract according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily practice the present invention. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention.

Preparation Example: Preparation of Extract

Graviola extract (S1) was prepared by extracting and filtering the dried and pulverized gravure using methanol as a solvent for 3 days according to the method for preparing a composition for preventing gray hair and promoting black hair induction. In addition, the extraction solvent was extracted by ethanol using black sesame seed, black ginseng, black rice, brown rice, Kratchaidam for 3 days, filtered and concentrated under reduced pressure to graviola extract (S1), black sesame extract (A), black ginseng extract (B), Black rice extract (C), brown rice extract (D), Krai chidam extract (E), banana peel extract (S2) was prepared.

In addition, each extract was prepared as an Example (EXCS), a mixed extract Example (MIXEE) as a single extract as shown in Table 1 below.

S1 A B C D E S1 EXCS 1 100 - - - - - - EXCS 2 - 100 - - - - - EXCS 3 - - 100 - - - - EXCS 4 - - - 100 - - - EXCS 5 - - - - 100 - - EXCS 6 - - - - - 100 - MIXEE 1 100 150 50 - - - - MIXEE 2 100 250 100 - - - - MIXEE 3 100 350 150 - - - - MIXEE 4 100 450 200 - - - - MIXEE 5 100 350 150 50 50 - - MIXEE 6 100 350 150 90 90 - - MIXEE 7 100 350 150 150 150 - - MIXEE 8 100 350 150 200 200 - - MIXEE 9 100 350 150 150 150 5 - MIXEE 10 100 350 150 150 150 10 - MIXEE 11 100 350 150 150 150 50 - MIXEE 12 100 350 150 150 150 70 - MIXEE 13 100 - - - - - 100

(Unit: parts by weight)

Experimental Example 1: Evaluation of White Hair Prevention and Black Hair Promotion Effectiveness

DPPH radical scavenging assay

Brand-Williams (Imai, J., Ide, N., Nagae, S., Moriguchi, T., Matsuura, H. and Itakura, Y. 1994.Antioxidant and radical scavenging effects of aged garlic extract and its constituents.Planta Med 60, 417-420.) Was modified to determine the free radical scavenging ability of Graviola extract (S1) against DPPH radical. At a final volume of 500 μl, the concentrations were 1, 2, 4, 8, 16, 32, and 64 μg / ml. Each sample was treated with the test concentration, mixed well, and reacted at room temperature for 1 hour for absorbance at 540 nm. Was measured. Vitamin C 100 μg / ml, well known as an antioxidant, was used as a positive control and measured and compared.

Referring to FIG. 1 below, it can be seen that free radical scavenging ability is generally increased in proportion to the concentration of S1. Accordingly, it can be seen that there is an antioxidant effect of the gravola extract (S1).

2.Reducing power assay

A reducing power test was performed to compare the ability to remove reactive oxygen species (ROS). Oyaizu (Oyaizu, M. 1986. Studies on products of browning reaction--antioxidative activities of products of browning reaction prepared from glucosamine.Jap. J. Ntr. Dia. 44, 307-315.) 6 μl of Fr. 7, Fr.8, 200 μl of 200 mM phosphatebuffer (pH 6.6) and 200 μl of 1% potassium ferricyanide are added and stirred and left at 50 ° C. for 20 minutes. Add 10% TCA to complete the reaction and centrifuge at 3000RPM for 10 minutes. Add 250 μl of distilled water and 1% ferric chloride to 250 μl of the supernatant. Samples subjected to this procedure are measured at absorbance of 620 nm. Positive control group was compared by using vitamin C 10 μg / ml. The results are shown in Figure 2 below.

Referring to FIG. 2 below, the gravure extract (S1) increased the reducing power in proportion to the concentration, compared to vitamin C (100μg / ml) compared to vitamin C (64μg / ml 209% 41% than vitamin C) It can be seen that it shows excellent reducing power.

3. In vitro measuring tyrosinase activity

To determine the degree of tyrosinase activity, the negative control group was compared with the sample. Add 237 μl of 0.1 M phosphate buffer (pH 6.5), 3 μl of Graviola extract (S1), and 20 μl of mushroom tyrosinase (1500 U / ml). 40 μl of 1.5 mM tyrosine solution was added to the solution and reacted at 37 ° C. for 15-20 minutes. The absorbance was measured at 490 nm using an ELISA reader (ELISA processor II. Behring, Germany). The results are shown in Figure 3 below.

Referring to FIG. 3 below, it can be seen that Tyrosinase activity is increased in proportion to the concentration of the gliola extract (S1).

4. In vitro Melanin synthesis measurement

L-DOPA and tyrosine as substrates and melanin synthesis using enzymes tyrosinase can be used to compare melanin synthesis efficacy. Add 455 μl of 0.1 M phosphate buffer (pH 6.5), 20 μl of L-DOPA, and 5 μl of Graviola extract (S1) to the test tube, add 20 μl of 2,000 U / ml mushroom tyrosinase, and react at 37 ℃ for 1 hour. Let's do it. And the absorbance was measured at 490 nm using an ELISA reader (ELISA processor II. Behring, Germany). The results are shown in Figure 4 below.

As a result, it can be seen that as the concentration of the gravola extract increases, the effect of promoting melanin synthesis is enhanced.

5. MTT assay

Cytotoxicity was measured according to the concentration through MTT assay. Hansen (Hansen, MB, Nielsen, SE and Berg, K. 1989. Re-examination and further development of a precise and rapid dye method for measuring cell growth / cell kill.J. Immunol.Methods. 119, 203-210.) Cytotoxicity of Graviola extract (S1) against B16F1 cells was measured using MTT (3- (4,5-dimethyl-2-yl) -2,5-diphenylterrazolium bromide). The results are shown in Figure 5 below.

Referring to FIG. 5 below, the gravure extract was increased in proportion to its concentration and cytotoxicity was 58% at a concentration of 2 μg / ml.

6. Measurement of Melanin Production in B16F1

The melanin content was measured using B16F1 cells to investigate the effect of melanin production on DOPA (dihydroxyphenylalanine) stimulation. Cells were aliquoted into 3 × 10 ⁵ cells / well in a 6-well plate. After 1 hour of treatment, the cells were cultured for 48 hours after stimulating melanin production with L-DOPA. Cells were collected, precipitated by centrifugation at 1,200 rpm for 5 minutes, and then dissolved in 1 ml homogenization buffer (50 mM sodium phosphate pH 6.5, 1% Triton X-100, 2 mM phenyl methyl sulfonyl fluoride). 200 μl of 1 N NaOH (10% DMSO) was added to the pellets, and the absorbance was measured at 405 nm after vortex. A standard calibration curve obtained from melanin standard (Sigma, USA) was used to calculate the amount of melanin produced in each well. Melanin was compared with melanin production in 10 ⁴ cells. The results are shown in Figure 6 below.

Referring to FIG. 6, negative control vitamin C, which is a negative control group, inhibited melanin synthesis, but the gravure extract showed an increase of 34% melanin synthesis at 4 μg / ml compared with the test group. .

7. Statistical Processing

Each experiment was repeated three or more times, and the results were expressed as mean ± standard deviation for each sample concentration. The significance difference test for each concentration group was considered to be statistically significant after p 's value of student's test compared to the control group.

[Experimental Example 2: Comparative Experiment of Melanin Synthesis with a Single Extract]

Graviola extract (S1), black sesame extract (A), black ginseng extract (B), black rice extract (C), brown rice extract (D), Kratchaidam extract (E) to test the effect promoting the melanin synthesis of each Experiments were conducted to compare the melanin synthesis promoting effect on the EXCS 1 to 6 contained in the same concentration (16 μg / ml) plant extracts. Experiment for the melanin synthesis promoting effect in the same manner as in vitro melanin synthesis measurement experiment in the same manner as Experimental Example 1 and the results are shown in Figure 7 below.

Referring to FIG. 7 below, it can be seen that in the above range, gravureola extract (S1) has the best effect of promoting melanin synthesis. In particular, since it shows an excellent melanin synthesis promoting effect even in a relatively low concentration range, it can be said that the effect of preventing gray hair and causing black hair is the most excellent.

[Experimental example: Comparative experiment of complex extract and melanin synthesis promoting effect]

In order to confirm whether the synergistic effect of the use of the composite extract and the critical significance of each content of the composite extract is shown by diluting each Example (MIXEE 1 to 13) equally to 16 μg in the same manner as in Experiment 1 In the same way as the in vitro melanin synthesis measurement experiments were carried out for the melanin synthesis promoting effect. The results are shown in the following [Table 2] indexed to be compared directly with Example EXCS 1 containing a gravure extract (S1). The higher the number, the better the melanan synthesis effect.

EXCS1 MIXEE 1 MIXEE 2 MIXEE 3 MIXEE 4 MIXEE 5 MIXEE 6 MIXEE 7 MIXEE 8 MIXEE 9 MIXEE 10 MIXEE 11 MIXEE 12 MIXEE 13 Melanogenesis 100 89 102 101 93 97 95 109 103 105 110 114 112 125

(Unit: exponent)

Referring to [Table 2], it can be seen that the melanin synthesis promoting effect is best when the banana peel extract (S2) is mixed when provided as a complex extract.

However, in the group except the banana peel extract (S2) it was confirmed that the synergistic effect on the melanin synthesis action is strongly shown as a synergistic effect by the interaction in MIXEE 7, 9 and 10 to 12. However, black sesame extract (A), black ginseng extract (B), black rice extract (C), brown rice extract (D), Kratchaidam extract (E) has a lower melanin synthesis promoting effect than the gravola extract (S1) In view of this, it can be seen that the synergistic effect is obtained when used as the complex extract, MIXEE 2, 3 also shows a significant excellent effect.

Melanocyte mechanism is the cause of the change of black hair to white hair as aging progresses. As catalase, methionine sulfoxide reductase A and B protein expression decreases, H ₂ O ₂ accumulates around hair follicles and the methionine residues of tyrosinase are involved in melanin synthesis. By oxidizing the enzyme structure is modified melanin synthesis is reduced to cause white hair.

Therefore, the gravureola extract according to the present invention was confirmed that the antioxidant effect is excellent and not only promote melanin synthesis, but also can effectively remove H ₂ O ₂ . Furthermore, when used as a complex extract, it can be seen that the melanin synthesis promoting effect is increased by more than a simple combination of individual extracts by the interaction of each extract.

Accordingly, when using the above embodiment it can be utilized in various products exhibiting the effect of causing black hair and preventing white hair.

Preparation Example 1 Shampoo Composition 1

Classification (% by weight) Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Sodium Lauryl Sulfate (30%) 20.0 20.0 20.0 20.0 Polyoxyethylene lauryl
Sodium sulfate (30%) 20.0 20.0 20.0 20.0 Palm oil fatty acid diethanolamide 2.0 2.0 2.0 2.0 Propylene glycol 2.0 2.0 2.0 2.0 Composition for preventing gray hair and promoting black hair, including gravola 0.001 0.01 0.1 One Pigmentation (blue 1) Quantity Quantity Quantity Quantity Paraoxybenzoic Acid Ester 0.2 0.2 0.2 0.2 Spices (Combined Flavor SKP-3473) Quantity Quantity Quantity Quantity Citric acid Quantity Quantity Quantity Quantity Purified water Remaining amount Remaining amount Remaining amount Remaining amount

 (Unit: weight%)

Preparation Example 3 Hair Dye Post-Treatment Composition

Ingredient Name Preparation Example 5 Example 6 Liquid paraffin 3.00 8.00 Cetostearyl alcohol 2.00 0.60 Behentrimonium chloride 1.0 2.5 Methylparaben 0.15 2.5 Composition for preventing gray hair and promoting black hair, including gravola 0.001 One Combination Quantity Quantity Purified water Remaining amount Remaining amount

 (Unit: weight%)

Preparation Example 3 Hair Gel Composition

Ingredient Name Preparation Example 7 Preparation Example 8 Preparation Example 9 Preparation Example 10 Composition for preventing gray hair and promoting black hair, including gravola 0.001 0.01 0.1 One ethanol 10 10 10 10 Acrylate polymer 2 3 4 5 Polyvinylpyrrolidone 0.2 0.2 0.2 0.2 Carboxy Vinyl Polymer 0.3 0.3 0.3 0.3 Triethanolamine 2 3 4 5 incense 0.2 0.2 0.2 0.2 Purified water Remaining amount Remaining amount Remaining amount Remaining amount

 (Unit: weight%)

Production Example 4 Preparation of Ointment

0.01% for gray hair prevention and black hair induction promoting composition comprising gravola, 0.01% of quercetin (Sigma), 8.0% of diethyl sebacate, 5.0% of light lead, polyoxyethylene oleyl-ether phosphate (EO 4) 6.0 %, Paraoxybenzoic acid ester appropriate amount, petrolatum residual amount can be prepared according to the usual ointment manufacturing method.

Preparation Example 5 Preparation of Soft Cosmetics

0.01%, quercetin 0.01%, glycerin 2.0%, hyaluronic acid 1.0%, polyoxyethylene oleyl ether (EO20) 0.1%, polyoxyethylene hardened castor oil EO40) 0.1%, the amount of paraoxybenzoic acid ester, the amount of perfume, the amount of pigment, and the amount of purified water can be prepared according to the conventional method for producing flexible cosmetic water.

Production Example 6 Preparation of Nutrients

0.01%, Quercetin 0.01%, Cetanol 1.0%, Beeswax 0.5%, Vaseline 2.0%, Squalane 6.0%, Ethanol 3.0%, 1,3-butylene glycol 4.0%, polysorbate 60 1.0%, sorbitan sesquioleate 0.3%, carboxy vinyl polymer 0.3%, triethanolamine 0.3%, paraoxybenzoic acid ester amount, flavor amount, pigment amount, purified water residual amount of normal nutrition It can manufacture according to a method.

Preparation Example 7 Preparation of Cream

0.01%, stearyl alcohol 6.0%, stearic acid 2.0%, concentrated glycerin 1.0%, squalane 9.0%, 1,3-butylene glycol 6.0%, polysorbate 60 1.5%, polyethylene glycol 1000 4.0%, cured lanolin 4.0%, octyl dodecanol 10.0%, sorbitan stearate 0.8%, triethanolamine 0.5%, paraoxybenzoic acid ester amount, flavor amount, pigment amount, purified water residual amount It can manufacture according to a manufacturing method.

Preparation Example 8 Preparation of Essence

Cyclomethicone 15.0%, caprylic capric triglyceride 3.0%, mineral oil 3.0%, beeswax 1.0%, cetyldimethicone polycopolyol 3.0%, containing gravure to prevent hair and hair loss promoting composition 1.0%, resvera Totol 0.001%, glycerin 5.0%, magnesium sulfate 3.0%, the amount of paraoxybenzoic acid ester, the amount of perfume, the amount of pigment, the remaining amount of purified water can be prepared according to the usual essence production method.

Preparation Example 9 Food Composition

Tea composition was prepared by mixing natural products as shown in Table 11 below using the composition for preventing gray hair and promoting black hair induction including gravure (MIXEE 11). The tea composition can be prepared according to conventional methods.

Production Example
9-1 Production Example
9-2 Production Example
9-3 Production Example
9-4 Production Example
9-5 Production Example
9-6 Production Example
9-7 Production Example
9-8 Production Example
9-9 MIXEE 11 100 100 100 100 100 100 100 100 100 Cherry extract - 15 - - 5 11 15 20 30 Thorn tree extract - - 50 - 40 50 70 80 100 Rooibos extract - - - 5 0.5 One 3 5 7

(Unit: parts by weight)

Ten samples were randomly selected and tasted according to the preparation example according to Table 9, and the flavors and tastes were subjectively evaluated to be expressed in an index of 1 to 10, and the results are shown in Table 10 below. The index shown in Table 12 below is that the higher the number is the better the flavor and taste. On the other hand, palatability is determined by the average value of the aroma and taste.

Production Example
9-1 Production Example
9-2 Production Example
9-3 Production Example
9-4 Production Example
9-5 Production Example
9-6 Production Example
9-7 Production Example
9-8 Production Example
9-9 incense 5 7 6 4 6 8 8 9 7 flavor 4 5 4 4 6 7 8 8 6

Referring to Table 12, it can be seen that the flavor and taste are excellent in the case of the preparation examples 9-6 to 9-8. Therefore, when provided as a food composition with a high palatability according to the above range it may be advantageous for the spread of the functional food composition of promoting hair follicles.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concepts of the present invention defined in the following claims are also provided. It belongs to the scope of rights.

Claims (9)

Graviola Extract; And
Additionally containing banana peel extract
Preventing gray hair and promoting hair growth. delete delete delete delete It comprises a composition for preventing white hair and black hair induction according to claim 1
Food composition for preventing gray hair and promoting hair loss. It comprises a composition for preventing white hair and black hair induction according to claim 1
Hair external preparation composition for the prevention of gray hair and the promotion of black hair. It comprises a composition for preventing white hair and black hair induction according to claim 1
Hair cleaning composition for preventing gray hair and promoting black hair. delete

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