KR101669626B1 - Composition for preventing or treating pigmentations - Google Patents

Abstract

The present invention relates to a composition for preventing and treating pigmentation including Morus alba L extract, Glycyrrhiza uralensis Fisch extract and Prunella vulgaris extract.
The composition for the prevention and treatment of pigmentation according to the present invention may comprise 50 to 167 parts by weight of Zygomycorrhizae extract and 17 to 133 parts by weight of the herbal extract, based on 100 parts by weight of the topical extract.
The upper limb extract, the licorice root extract and the chrysanthemum extract may be the upper limb, the licorice root, and the liquor extracted 10 to 15 times by weight of the dry weight of the chrysanthemum. The upper, upper, and lower chrysanthemum extracts may be obtained by mixing the upper, middle, and lower chow with each other and extracting for 10 to 20 hours, filtering, concentrating and drying. The composition for preventing or treating pigmentation may be an oral composition or an external preparation. The oral composition may be a food or health functional food consisting of one formulation selected from the group consisting of tablets, microcapsules, suspensions, solutions and powders.
INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a composition for the prevention and treatment of pigment deposition showing an excellent effect on whitening action and antioxidation action on skin.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing or treating pigmentation,

TECHNICAL FIELD The present invention relates to a composition for preventing and treating pigmentation, and more particularly, to a composition for prevention and treatment of pigmentation which is excellent in whitening action and antioxidation effect on skin by containing a natural extract.

The blackening phenomenon caused by overproduction of the melanin pigment and skin deposition is due to the activation of tyrosinase present in the melanosome in the melanocyte. Melanin protects the skin from ultraviolet rays or external stimuli, but when it is synthesized more than necessary, it is over-deposited on the skin to form spots and freckles. Melanin is first produced by enzymes such as tyrosinase, tyrosinase related protein-1 (TRP-1) and DCT (dopachrome tautomerase) in melanosomes in melanocytes in the basal layer of the epidermis. In particular, tyrosinase Is a key enzyme involved in the first step of melanin production. Tyrosine is converted to DOPA quinone via DOPA and is converted to dopaquinone by automatic oxidation and enzyme reaction DOPAchrome) to produce melanin as a dark brown copolymer.

Korean Patent No. 1338546 relates to a whitening composition having an effect of inhibiting the production of melanin. Such a conventional whitening functional composition is excellent in whitening function and preference because it is an effective ingredient of a plant-derived extract. However, since the use thereof is limited so that it can not be edible and can only be used as an external preparation, It is difficult to utilize the active ingredients in various ways.

Accordingly, there is a demand for development of a whitening functional food material which is excellent in whitening function and can prevent or treat skin pigmentation.

Korean Patent No. 1338546

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in view of the above problems, and it is an object of the present invention to provide a skin coloring composition containing a topical extract, And to provide a composition for prevention and treatment.

It is an object of the present invention to provide a composition for preventing and treating pigmentation, which is a whitening functional food material which can be used as an oral composition.

In order to achieve the above object, the composition for preventing and treating pigmentation according to the present invention comprises Morus alba L. extract, Glycyrrhiza uralensis Fisch extract and Prunella vulgaris extract.

The composition for the prevention and treatment of pigmentation according to the present invention may comprise 50 to 167 parts by weight of Zygomycorrhizae extract and 17 to 133 parts by weight of the herbal extract, based on 100 parts by weight of the topical extract.

The upper limb extract, the licorice root extract and the chrysanthemum extract may be the upper limb, the licorice root, and the liquor extracted 10 to 15 times by weight of the dry weight of the chrysanthemum.

The upper, upper, and lower chrysanthemum extracts may be obtained by mixing the upper, middle, and lower chow with each other and extracting for 10 to 20 hours, filtering, concentrating and drying.

The composition for preventing or treating pigmentation may be an oral composition or an external preparation.

The oral composition may be a food or health functional food consisting of one formulation selected from the group consisting of tablets, microcapsules, suspensions, solutions and powders.

The external preparation may consist of a single formulation selected from the group consisting of solutions, lotions, creams, powders, and cakes.

According to the composition for preventing and treating pigmentation according to the present invention, it exerts an excellent effect on skin whitening action and antioxidant action.

 INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a composition for preventing or treating pigmentation which can be orally administered, by providing a whitening-functioning material derived from a plant capable of being edible.

1 is a graph showing the DPPH radical scavenging activity of a composition for preventing and treating pigmentation according to an embodiment of the present invention.
FIG. 2 is a graph showing the evaluation result of inhibition of tyrosinase activity of a composition for preventing or treating pigmentation according to an embodiment of the present invention. FIG.
3 is a graph showing the results of evaluation of L-DOPA activity of a composition for preventing and treating pigmentation according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Note that, in the drawings, the same components are denoted by the same reference symbols as possible. Further, the detailed description of known functions and configurations that may obscure the gist of the present invention will be omitted. For the same reason, some of the components in the drawings are exaggerated, omitted, or schematically illustrated.

The composition for the prevention and treatment of pigmentation according to the present invention comprises an upper body extract, a licorice root extract and a chrysanthemum extract, wherein the upper chrysanthemum extract, The mixture is mixed with the alcohol in a volume ratio of 1: 1 to 10, extracted at room temperature for 10 to 20 hours, filtered, concentrated under reduced pressure, and dried.

The upper limb is a medicinal herb that dries the Morus alba L. or the young branches of the copepods. The upper limb is usually cut in the sun and dried in the sun before it leaves in spring.

Glycyrrhiza uralensis is the roasted licorice, and it is believed that the roasted beef giblets, umbilical cord stones, umbilical cord muscles, long stomach muscles, It is a medicinal substance that has the efficacy that it is the efficacy which is both hematocrit (阴 阴 血), 祭 heat (邪 热 热), bacillus (肝 脉), 竹 表 寒,

And purple cleanse the liver (肝 气) and release the 結 (結結) with the fruit of Prunella vulgaris var. Aleutica, Prunella vulgaris var. Asiatica, Prunella vulgaris var. Lilacina for albiflora, , Acute mastitis, breast cancer, night-time eye pain, difficult to see light and tears, dizziness of head and eye, and Guan Wasa.

The alcohol is also referred to as ethyl alcohol or ethanol, and the alcohol which is the main component of alcohol is contained in various alcoholic beverages. It is a colorless liquid with a peculiar smell and taste and serves as a solvent for extracting the upper part, the licorice, and the persimmon, and preferably has a concentration of 70 to 90%.

More specifically, a 95% fermented juice is mixed with purified water and diluted to a concentration of 70 to 90%. Then, the upper and the lower potato are usually fed at a rate of 10 times the dry weight and 15 times the dry weight, 10 to 20 hours, and filtered using a cartridge filter. The filtrate is concentrated under reduced pressure at a temperature of 60 ° C or less to concentrate the concentrate so that the concentration of the concentrate is 20% or less and freeze-dried at 45 ° C or less to obtain a dried upper limb extract,

The resulting dried, upper, lower, and supernatant extracts may be mixed with each other to obtain a dry composite (hereinafter referred to as SDW 1). The mixing ratio of the upper limb extract, the licorice root extract and the chrysanthemum morifolium extract was higher than the combination ratio of tyrosinase inhibition rate (%), L-DOPA inhibition rate (%) and DPPH inhibition rate (% (response surface modle equation). Accordingly, the composition for preventing and treating pigmentation according to an embodiment of the present invention may comprise 50 to 167 parts by weight of Zygomycorrhizae extract and 17 to 133 parts by weight of an extract of Chrysanthemum morifolium, based on 100 parts by weight of the topical extract. More specifically, it is preferable from the viewpoint of efficiency of injection to include a topical extract, a licorice extract and an acaricus extract in a weight ratio of 1.5 to 3.0: 1.5 to 2.5: 0.5 to 2.0.

The results of the reaction surface analysis according to the central composite design for deriving the blending ratio of the SDW1 complex of the upper, lower, and upper extracts according to the embodiments of the present invention are shown in Table 1 below.

No
Independent variable Dependent variable Upper limb extract ratio Percentage of licorice extract And the extract fraction Inhibition rate of tyrosinase (%) L-DOPA inhibition rate (%) DPPH Inhibition (%) One 1.5 (-1) 2 (-1) 1 (-1) 48.83 43.76 33.72 2 1.5 (-1) 2 (-1) 2 (1) 31.09 45.02 37.77 3 1.5 (-1) 4 (1) 1 (-1) 51.31 45.20 27.77 4 1.5 (-1) 4 (1) 2 (1) 36.58 38.39 32.09 5 2.5 (1) 2 (-1) 1 (-1) 61.91 48.00 30.35 6 2.5 (1) 2 (-1) 2 (1) 39.34 43.25 36.80 7 2.5 (1) 4 (1) 1 (-1) 58.65 45.58 28.24 8 2.5 (1) 4 (1) 2 (1) 48.63 41.48 32.89 9 2 (0) 3 (0) 1.5 (O) 52.88 41.73 32.55 10 2 (0) 3 (0) 1.5 (O) 53.63 43.43 31.90 11 2 (0) 3 (0) 1.5 (O) 52.11 43.82 30.77 12 1 (-2) 3 (0) 1.5 (O) 31.90 36.49 34.39 13 3 (2) 3 (0) 1.5 (O) 61.24 47.25 31.03 14 2 (0) 1 (-2) 1.5 (O) 51.78 43.44 34.64 15 2 (0) 5 (2) 1.5 (O) 37.77 40.85 30.12 16 2 (0) 3 (0) 0.5 (-2) 60.32 42.95 26.74 17 2 (0) 3 (0) 2.5 (2) 31.68 29.99 35.32

In the central synthesis plan, the weight ratio of the upper, middle, and lower extracts as the independent (conditional) variables was coded in five steps of -2, -1, 0, 1, and 2 and set to 17 intervals according to the central synthesis plan .

(%), L-DOPA inhibition rate (%) and DPPH inhibition rate (%), which are actually active indicators of pigment deposition, as the dependent variables The experiment is repeated three times and the mean value is used for the regression analysis. The SAS program (Statistical Analysis System program, version 9.1) was used to predict the optimal conditions by regression analysis, and a Mathematica program was used to plot the 4-dimensional response surface for each experimental condition.

The resulting response surface model equation is shown in Table 2 below.

Dependent variable Secondary polynomials obtained through reaction surface analysis ¹⁾ R ² valence Tyrosinase
Inhibition rate (%) Y _tyr = 8.171141 + 39.539565X ₁ + 6.539837X ₂ -5.955326X ₃ -6.392391X ₁ ² -0.485000X ₁ X ₂ -2.046848X ₂ ² -0.060000X ₁ X ₃ + 3.890000X ₂ X ₃ -6.962391X ₃ ² 0.9201 0.0043 L-DOPA
Inhibition rate (%) Y _dopa = 18.956359 + 7.587935X ₁ + 1.871413X ₂ + 21.847826X ₃ -0.607609X ₁ ² + 0.250000X ₁ X ₂ -0.083152X ₂ ² -1.650000X ₁ X ₃ -1.855000X ₂ X ₃ -6.007609X ₃ ² 0.7521 0.1353 DPPH
Inhibition rate (%) Y _dpph = 47.727500-11.698750X ₁ -4.986875X ₂ + 4.871250X ₃ + 1.055000X ₁ ² + 1.402500X ₁ X ₂ + 0.181250X ₂ ² + 1.365000X ₁ X ₃ -0.382500X ₂ X ₃ -0.625000X ₃ ² 0.9441 0.0016

1) X ₁ , weight ratio of upper limb extract; X ₂ , weight ratio of the licorice extract; X ₃ , and the weight ratio of the herbal extract

In this case, the mixing ratio of the top, bottom, and top extracts is in the range of 1.5 to 3.0, 1.5 to 2.5, and 0.5 to 2.0, respectively. Table 3 shows the results of comparison between the expected level and the actual level of the preventive / therapeutic efficacy of the pigmentation in the blending zone obtained through the reaction surface analysis.

No. X ₁ X ₂ X ₃ inhibition (%) tyrosinase L-DOPA DPPH Remarks Upper limb Syrup And seconds One 1.70 2.80 1.80 Estimate 50.00 44.00 33.00 RSM (middle) Found 53.29 53.05 32.82 2 2.57 2.37 0.74 Estimate 67.09 - - tyrosinase
Maximum value Found 65.95 58.22 27.09 3 2.90 3.12 1.06 Estimate - 48.33 - L-DOPA
Maximum value Found 58.65 59.08 26.12 4 1.55 1.50 1.99 Estimate - - 33.58 DPPH
Maximum value Found 45.25 49.64 32.74

As shown in Table 3, when the composition for prevention and treatment of pigmentation according to the example of the present invention contains 2.57: 2.37: 0.74 weight ratio of top, twig, and chrysanthemum extracts, 65.96% of tyrosinase inhibition Activity, 58.22% L-DOPA inhibitory activity, and 27.09% DPPH inhibitory activity.

Accordingly, the composition for prevention and treatment of pigmentation according to the present invention is characterized in that it contains Morus alba L extract, Glycyrrhiza uralensis Fisch extract, and Prunella vulgaris extract in a weight ratio of 1.5 to 3.0: 2.5: 0.5 to 2.0, and most preferably 2.57: 2.37: 0.74 in terms of weight ratio.

The composition for preventing and treating pigmentation according to an embodiment of the present invention is characterized by being an oral composition or an external preparation. The oral composition may be one or more selected from the group consisting of tablets, microcapsules, suspensions, solutions and powders The composition may be a food or a health functional food comprising a formulation, and the external preparation preferably comprises one formulation selected from the group consisting of solutions, lotions, creams, powders, and cakes. Since Morus alba L., Glycyrrhiza uralensis Fisch, and Prunella vulgaris, which are included as extracts in the examples of the present invention, are edible plants, the present invention The composition for preventing and treating pigmentation according to the embodiment of the present invention may be most preferably used as a material for producing an oral composition.

Hereinafter, the composition for preventing and treating pigmentation according to the present invention will be described with reference to Examples and Experimental Examples. The results for Experimental Examples 1 to 3 are shown in Figs.

<Examples>

Add 1 kg of superfine, 1 liter of syrup, and 5 liters of syrup (80%) into a stainless steel extraction container and extract it at room temperature for 18 hours. Filter it first using filter paper, then filter it with filter paper. The filtered filtrate was concentrated under reduced pressure at a temperature of 50 캜 and powdered by freeze drying to prepare a top extract, a licorice extract and an acorn extract. The weight ratio of the top extract to the extract was 1.5 to 3.0, the weight of the extract was 1.5 to 2.5, (Weight ratio of extract: 0.5 to 2.0) were mixed to prepare a complex (hereinafter referred to as SDW1). The resulting extract and complex were dissolved in distilled water or dimethyl sulfoxide (DMSO) at a concentration of 1 μg / ml, 5 μg / ml, 10 μg / ml, 50 μg / ml, 100 μg / ml and 200 μg / Respectively.

<Experimental Example 1>

In Experimental Example 1, the DPPH radical scavenging ability was evaluated. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) itself is a compound having free radicals, and when it binds to a hydrogen group, its specific hue decreases and it is used to evaluate antioxidant activity. The test substance was dissolved in distilled water or dimethyl sulfoxide (DMSO) to evaluate the antioxidant activity using this principle. The test substance was used by using 0.1 mM DPPH (D-9132, SIGMA) or L-ascorbic acid (A5960, SIGMA) Respectively. For the specific test method, 0.1 mM DPPH was dissolved in methanol, and 150 μL of DPPH solution was added to 50 μL of the sample dissolved in concentration. The reaction was carried out at room temperature (dark room) for 30 minutes and then at 570 nm. The free radical scavenging activity .

As a control substance, ascorbic acid was used. Experiments were carried out using SDW 1, which is a mixture of the upper, upper, and lower extracts, as a test substance. The test results are shown in Fig.

As a result, the antioxidative activity of 95.23% in 25 μg / ml ascorbic acid and 90.16% in 12.5 μg / ml ascorbic acid as the control group was used as a standard. The antioxidant activity of the extracts of the upper and upper extracts, which were the single extract, was 44.72% and 49.77% at 100 μg / ml, respectively, and 33.88% of 3.1 μg / ml ascorbic acid. The extracts of E. coli showed 97.50% and 83.53% at 100 μg / ml, respectively, and showed antioxidant activity corresponding to 25 μg / ml and 12.5 μg / ml of ascorbic acid as the control group. The antioxidant activity of SDW 1, which is a combination of the upper, upper, and lower extracts, was 64.51%, which was equivalent to about 33.88% of 3.1 μg / ml ascorbic acid. Therefore, the antioxidant activity of SDW 1, which is a combination of 100 μg / ml and the extract, is expected to be superior. Therefore, the composition for preventing and treating pigmentation according to the embodiment of the present invention shows excellent antioxidative activity as a result of evaluation of DPPH radical scavenging ability, and thus it is considered that it is effective for prevention or treatment of skin pigmentation.

<Experimental Example 2>

In Experimental Example 2, inhibition of tyrosinase activity was evaluated. Mushroom tyrosinase (500 units / mL, Sigma Co., St. Louis, MO, USA) was added to 120 μL of 0.1 M potassium phosphate buffer (pH 6.8) 20 μL of the solution was added, and 40 μL of 1.5 mM tyrosine was added, followed by reaction at 37 ° C. for 15 minutes. The absorbance was measured at 450 nm after the reaction and the inhibitory effect of tyrosinase activity was calculated as follows. The results of the analysis are shown in Fig.

The concentration of kojic acid, which is well known for its whitening effect, was 31.71% at 2.1 μg / ml, 45.73% at 4.2 μg / ml, 67.35% at 8.4 μg / ml and 84.69% at 16.6 μg / % And 33.3 μg / ml, respectively, of the inhibitory activity of tyrosinase activity of 92.58%. The single extract, topical extract, showed 96.0% and 94% inhibition of tyrosinase activity at a concentration of 100 μg / ml and 50 μg / ml, respectively, indicating that tyrosinase equivalent to kojic acid at a concentration of 33.3 μg / ml The activity of inhibiting the activity of the enzyme was confirmed. The results showed that the extracts of 100 μg / ml of Rhizopus japonica extract inhibited tyrosinase activity in the order of 68.00% and 37.30%, respectively. The extracts of Rhizoma extract at 50 μg / ml showed 34.21% And 36.8% of the extracts inhibited tyrosinase activity.

In addition, SDW 1, a complex of upper, upper, and lower extracts, showed 82.50% and 77.90% inhibition of tyrosinase activity at 100 μg / ml and 50 μg / ml, respectively. Therefore, it was concluded that the whitening effect was superior to that of 50 μg / ml and 100 μg / ml of the upper limb extract, 100 μg / ml of the licorice extract, and 50 μg / ml and 100 μg / ml of SDW 1.

Therefore, the composition for preventing and treating pigmentation according to the embodiment of the present invention shows excellent inhibitory effect on tyrosinase activity as a result of inhibition of tyrosinase activity, and thus it is considered that it is effective for preventing or treating skin pigmentation.

<Experimental Example 3>

In Experimental Example 3, the activity of L-DOPA was evaluated. Add 10 μL of the sample solution and 210 μL of mushroom tyrosinase (500 units / mL, Sigma Co., St. Louis, MO, USA) solution to 210 μL of 0.1 M potassium phosphate buffer (L-DOPA (L-3,4-dihydroxyphenylalanine) solution) was added thereto, followed by reaction at 37 ° C for 5 minutes. After the reaction, the absorbance at 450 nm was measured. The results of the analysis are shown in Fig.

As a result, the concentration of nuruk acid was found to be 40.41% at the concentration of 2.1 μg / ml, 62.06% at the concentration of 4.2 μg / ml, 76.08% at the concentration of 8.4 μg / ml, 83.39% and 33.3 μg / ml at the concentration of 16.6 μg / The inhibitory effect of L-DOPA activity of 89.12% was confirmed. In the case of single extract, L-DOPA activity was inhibited by 78.37% and 74.06% at 100 μg / ml and 50 μg / ml, respectively. This result was similar to the effect of 8.4 μg / ml kojic acid on L-DOPA activity. Next, the inhibitory effect of L-DOPA was inhibited by 45.95% of 100 μg / ml of Ganoderma lucidum extract and 37.88% of Ganoderma lucidum extract. In addition, SDW 1, a complex of upper, upper, and lower extracts, showed 67.68% and 54.14% at 100 μg / ml and 50 μg / ml, respectively, showing a relatively high inhibitory effect on L-DOPA activity. Therefore, it was confirmed that the inhibitory effect of L-DOPA was superior to that of SDU-1, which is a combination of 50 μg / ml and 100 μg / ml of the upper limb extract. The results of inhibition of L-DOPA activity were also observed at the same concentrations and samples as the results of inhibition of tyrosinase activity of SDW 1, a mixture of upper and lower limbs at concentrations of 50 μg / ml and 100 μg / ml, .

Therefore, as a result of the L-DOPA activity evaluation, the composition for preventing and treating pigmentation according to the embodiment of the present invention shows excellent effect of inhibiting oxidation of L-DOPA, and thus it is considered to be effective for prevention or treatment of skin pigmentation.

It should be noted that the embodiments of the present invention disclosed in the present specification and drawings are only illustrative of the present invention in order to facilitate the understanding of the present invention and are not intended to limit the scope of the present invention. It will be apparent to those skilled in the art that other modifications based on the technical idea of the present invention are possible in addition to the embodiments disclosed herein.

Claims (7)

For 100 parts by weight of the upper part (Morus alba L) extract,
50 to 167 parts by weight of Glycyrrhiza uralensis Fisch extract, and
And 17-133 parts by weight of Prunella vulgaris extract
Wherein the composition is used for preventing or treating skin pigmentation. delete The method according to claim 1,
The composition for oral administration for preventing and treating skin pigmentation according to any one of claims 1 to 3, wherein the topical extract, Zygomycorrhizae extract and Zygomycorrhizae extract are extracted with a 10 to 15 times by weight weight of the top, The method according to claim 1,
Wherein the topical extract, Zygomycorrhizae extract and Zygomycorrhizae extract are prepared by mixing the top, bottom, and side of each of the top, bottom, and both of the extracts together with a spatula, extracting it for 10 to 20 hours, filtering, concentrating and drying the skin pigment. &Lt; / RTI &gt; delete The method according to claim 1,
Wherein the composition for oral administration comprises one formulation selected from the group consisting of tablets, microcapsules, suspensions, solutions and powders. delete

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