KR101904800B1 - Cosmetic composition for anti-oxidation or skin whintening containing extract of medicinal herbs mixture - Google Patents

Abstract

The present invention relates to a cosmetic composition which is excellent in skin whitening efficacy by preventing antioxidation or melanin pigment deposition which can eliminate free radicals caused by active oxygen generated by various physical, chemical and environmental factors, and more particularly, The present invention relates to a cosmetic composition which is excellent in antioxidative effect by inhibiting oxidation by active oxygen in the skin by containing a mixed extract and is excellent in whitening effect by inhibiting deposition of skin melanin pigment.

Description

TECHNICAL FIELD [0001] The present invention relates to an antioxidant or whitening cosmetic composition containing an extract of a herbal medicinal ingredient,

The present invention relates to a cosmetic composition which is excellent in skin whitening efficacy by preventing antioxidation or melanin pigment deposition which can eliminate free radicals caused by active oxygen generated by various physical, chemical and environmental factors, and more particularly, The present invention relates to a cosmetic composition which is excellent in antioxidative effect by inhibiting oxidation by active oxygen in the skin by containing a mixed extract and is excellent in whitening effect by inhibiting deposition of skin melanin pigment.

Active oxygen generated by various physical, chemical and environmental factors such as in vivo enzymes, reductive metabolism, chemicals, pollutants and photochemical reactions can cause non-selective and irreversible destruction of cellular components such as lipids, proteins, It is known to cause various diseases including cancer and senescence. In addition, various peroxides such as lipid peroxides produced as a result of lipid peroxidation by these active oxygen also cause oxidative destruction to cells and cause various dysfunctions, thereby causing various diseases. Accordingly, antioxidants such as free radical scavengers or peroxidation-inhibiting substances are expected to be used as agents for inhibiting or treating various diseases caused by these oxides.

On the other hand, various factors are involved in determination of human skin color. Among them, activity of melanocyte which makes melanin pigment, distribution of blood vessels, thickness of skin, presence of pigment inside and outside of human body such as carotinoid and bilirubin The factors of importance are.

Especially, the most important factor is melanin, a melanin pigment produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of melanin pigment affects genetic factors, hormonal secretion, physiological factors such as stress, and environmental factors such as ultraviolet irradiation.

The melanin pigment produced in melanocytes of body skin is a phenolic polymer substance having a complex form of black pigment and protein. It protects the subcutaneous skin organs by blocking ultraviolet rays irradiated from the sun, and at the same time, free radicals And protects proteins and genes in the skin.

Melanin, which is produced by stress stimuli inside and outside of the skin, is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stress disappears. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results.

In addition, as the number of people enjoying outdoor activities increased due to the increase in the leisure population, the demand for preventing melanin pigmentation due to ultraviolet rays increased.

In response to such demands, substances having ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, derivatives thereof, or thyrocinase inhibiting activity have been conventionally used in cosmetics or pharmaceuticals, The use thereof is limited due to insufficient whitening effect, safety problem to skin, formulation and stability of formulation in cosmetics, and the like.

Seeshin is a perennial herbaceous plant belonging to the rat and bell. There are many nodes in the rhizome, and two leaves come out from the end of the main stem. It is a medicinal herb that dried the roots of bamboo shoots. It is named after the roots are extremely thin and very spicy. It was called "Shin-myeon" in the name of Lee Duk-myun, and it was also called "poetry", "Sosin" and "Seko". Leaves are heart-shaped and have a width of 5 to 10 cm with flat edges. The flower is reddish purple, and one leaf comes out when it is going to come out. Fruit is long bract, with lanceolate on the end and about 20 seeds. Root is collected in May-July and dried in the shade. The weakness is mild and the taste is spicy. The efficacy of the cold is blocked by the nose or runny nose continues to flow through the throat discharge of the secretion of perspiration when the sweating is not good, and the virus out of the body. It is also critical when the fever is severe with a cold, especially when the headache is severe and can not withstand. Chronic bronchitis or bronchiectasis due to a large amount of sputum, while coughing severely when the jinhae also acts. In addition, it will be healed if it is made into powder for stomatitis. However, it should not be used when weakness, weakness, sweating, anemia, or headache are present. In the private sector, it is used as an analgesic because it becomes pain when it is bitten in a sick place. A typical prescription is Sechin-tang.

It is a perennial herbaceous plant belonging to the mountain type. The name is Cunidium officinale MAKINO. It is a Chinese plant and it is commonly cultivated in Korea. Its height is 30 ~ 60㎝, it grows straight and branches are split. Leaves are alternate phyllotaxis, and the leaves are long and petiole is long. Leaves are gradually smaller as they go up, and the lower part is wrapped around the main stem as leaf leaves. Leaves are ovate or lanceolate, There are sawtooths with missing teeth and sharp sawtooths. Flowers bloom in August, and large phloem episodes develop at the ends of the branches and the main stem. The young seeds eat as herbs and the rootstock is dried and used as medicinal materials. The ingredient contains a large amount of aromatic essential oil, so it has a good smell. The main components are cnidilide, neocnidilide, and ligustilide. In pharmacological experiments with animals, it was found to have a sedative effect on the central nervous system, and a weak but sustained hypotensive effect was also confirmed. In the experiment on smooth muscle, the contraction power of the uterus was increased, and the sterilizing action was shown to have the effect of inhibiting the development of Escherichia coli, Dysentery, Pseudomonas aeruginosa, and Dermatophyte. This medicine is mild and tasteless, it enters the body and makes the circulation of blood vigorous and calms the pain. Therefore, it is often applied to headache. For her, she removes menstrual irregularities, dysmenorrhea, abdominal pain and dizziness. In addition, when the liver function is activated and anemia is taken, the hematopoietic effect is obtained. And the heart muscle pain caused by the contraction of the heart muscle is also improving symptoms. If the skeleton of the lower back and legs is weak and the person often causes muscle spasms, such symptoms will be improved. Reluctant to blend with Huang Rin (黄连) and take a long time to damage the spirit. In the private sector, they are kept in the closet to prevent mosquitoes. As a typical prescription, there is a mungwangtang (芎 帰 湯), which is used after the dissolution of his wife. It is one of the most used medicine in one room.

The passerby belongs to the rose family of Chinese origin Prunus mandshurica var . glabra , Prunus mandshurica , Prunus It is a dry seed of sibirica . Because it is Jinhae, goddess effect, it is used for medicine of relieving, asthma, bronchitis, acute hepatitis in private. Calcium, sodium, fiber, vitamins A, B and C are the main components of passerine, and they contain citric acid, malic acid, amigalline, and olein. It moisturizes and nourishes the rough and dry skin, and has fine lines, spots, freckles, whitening, exfoliating effect. There are more than 200 treatments using apricot seeds in the herbaceous gangmok (herbaceous seed), and they are often called 'apricot', because they have many uses and medicinal effects. Skin pigmentation, swelling, swelling and so on.

As a result of searching for natural antioxidants and whitening raw materials, the present inventors searched for natural products, and found that the herbal extracts of Seosin, Seungkung, And thus the present invention has been completed.

Accordingly, it is an object of the present invention to provide a cosmetic composition containing an extract of Sezin, Astragalus membranaceus, and Angela giganteus as active ingredients. Yet another object of the present invention is to provide a cosmetic composition for antioxidation, which contains an extract of Sezin, Astragalus membranaceus and Angelicae Radix as an active ingredient. Yet another object of the present invention is to provide a cosmetic composition having a free radical scavenging ability, which comprises an extract of Sezin, Astragalus membranaceus and Angelica keiskei as an active ingredient. Another object of the present invention is to provide a cosmetic composition having a skin whitening effect by containing an extract of Sezin, Astragalus membranaceus and Candida extract as an active ingredient.

In order to attain the above object, the present invention provides a cosmetic composition comprising an acanthomex extract, a cinnabar extract, and an extract from a passerine as an active ingredient.

Hereinafter, the present invention will be described in more detail.

The sesquiterpenoid extract, astragalus extract, and passerine extract of the present invention can be obtained according to a method common in the art, and the method is not particularly limited. Specifically, ethanol is further added to the dried sesquinia or cinnabar or passerine to extract an oil-soluble component, followed by reflux extraction, immersion, filtration and centrifugation to separate the filtrate under reduced pressure, An extract can be obtained.

The total amount of the sesquin, licorice extract and callus extract of the present invention is 0.001 to 20 wt%, more preferably 0.005 to 10 wt%, based on the total weight of the composition. If it is less than 0.001%, a desired effect can not be expected. If it is more than 20% by weight, there is a risk of denaturation of cosmetics such as rinsing, viscosity control of cosmetic formulations is not easy, and since a phase is liable to occur during preparation of emulsion formulations, Production economics also fall.

The mixing ratio of citrine, astragalus and goat extract contained in the cosmetic composition of the present invention is 1: 1 to 3: 1 to 3. It is difficult to expect the synergistic effect of the components if they exceed the mixing ratio.

The cosmetic composition provided by the present invention contains a mixture of the sesquin extract and the cynomolgus bark extract as described above, thereby preventing the skin oxidation reaction induced from ultraviolet rays and other external harmful components and inhibiting the deposition of the skin melanin pigment, Can be practically implemented.

The cosmetic composition of the present invention can be used in cosmetics for skin antioxidation and whitening, and is not particularly limited in its formulation. Examples of such cosmetic compositions include emulsions, creams, lotions, essences, packs, gels, powders, lipsticks, makeup bases, Lotion or skin adhesive type cosmetics, or a transdermal dosage form.

In the present invention, a cosmetic composition containing a mixture of sesquin, celadon and passerine may exhibit a whitening effect due to an effect of improving antioxidative and ultraviolet rays (UV) -containing pigment deposits with DPPH production inhibitory effect Respectively. Therefore, according to the present invention, the mixed extract of Sezin, Tongkung, and Candy can be very usefully used as a cosmetic composition or a pharmaceutical composition for antioxidant, whitening.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples, but the scope of the present invention is not limited thereto.

[ Comparative Example  1] Preparation of sesquic acid extract

To 300 g of dried sesquin were added 2 L of 70% ethanol, refluxed three times, and then immersed at 15 DEG C for 1 day. Then, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 24 g of an extract.

[ Comparative Example  2] Manufacture of astragalus extract

To 300 g of dried cilia, 2 L of 70% ethanol was added, and the mixture was refluxed three times and then immersed at 15 DEG C for 1 day. Then, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to obtain 33 g of an extract.

[ Comparative Example  3] Production of passerine extract

To 300 g of the dried dish, 2 L of 70% ethanol was added, and the mixture was refluxed three times and then immersed at 15 DEG C for 1 day. Then, the filtrate and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 35 g of an extract.

[ Example  1] Manufacture of mixed extract of sezin

20 g of the sesquicin extract of Comparative Example 1, 20 g of the cucurbitaceae extract of Comparative Example 2 and 20 g of the extract of Comparative Example 3 were mixed and then 400 ml of 70% aqueous ethanol solution was completely dissolved therein, followed by immersion for one day at 15 ° C. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to obtain 46 g of a mixture of seshinum, astragalus and goat extract.

[ Example  2] Manufacture of mixed extract of Sezin, Taeunggung, and passerine

12 g of the sesquicin extract of Comparative Example 1, 36 g of the cucurbitaceae extract of Comparative Example 2 and 12 g of the extract of Comparative Example 3 were mixed, 450 ml of a 70% aqueous ethanol solution was completely dissolved therein, and the mixture was immersed at 15 캜 for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 49 g of a mixture of the extract of Seishin, Taeungguk, and Raney.

[ Example  3] Manufacture of mixed extract of sezin

12 g of the cetin extract of Comparative Example 1, 12 g of the cinnabar extract of Comparative Example 2, and 36 g of the extract of Comparative Example 3 were mixed, 450 ml of a 70% aqueous ethanol solution was added thereto, and the mixture was completely dissolved and immersed at 15 캜 for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 48 g of a mixture of Seishin, Tongkung, and Candy Extract.

[ Example  4] Manufacture of mixed extract of sezin

8.5 g of the sesquicin extract of Comparative Example 1, 25.5 g of the cucurbitaceae extract of Comparative Example 2, and 25.5 g of the extract of Comparative Example 3 were mixed and then 450 ml of a 70% aqueous ethanol solution was completely dissolved therein, followed by immersion for one day at 15 ° C. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 48 g of a mixture of Seishin, Tongkung, and Candy Extract.

[ Test Example  1] Antioxidant effect test ( DPPH test )

The antioxidant activity was evaluated by the change of the absorbance generated by the reduction of the organic radical, DPPH (1,1-diphenyl-2-picryl hydrazyl) (the antioxidant was oxidized). The degree to which the oxidation of DPPH was suppressed and the absorbance was decreased as compared with the control group was measured by the following examples, and the concentration showing an absorbance of 50% or less as compared with the absorbance of the control group was evaluated as the effective antioxidant concentration.

The reaction solution was prepared by adding 190 μl of 100 μM (in ethanol) DPPH solution, 10 μl each of the comparative sample, the extracts of Examples 1 to 4 and the comparative sample and the control sample, reacting at 37 ° C. for 30 minutes, and measuring the absorbance at 540 nm. As a control sample, a synthetic antioxidant Trolox, which is widely used, was used.

 The results of DPPH analysis of each material are shown in Table 1 below.

DPPH analysis result (% inhibition) (IC50: sample concentration when absorbance was reduced by 50% by the added sample) Test substance IC50 (ppm) Trolox 55 Comparative Example 1 Sesin extract 125 Comparative Example 2 Cinnabar extract 97 Comparative Example 3 Passive extract 99 Example 1 Seisin, Taeunggung, Chungbuk mixed extract
(Mixing ratio = 1: 1: 1) 53 Example 2 Seisin, Taeunggung, Chungbuk mixed extract
(Mixing ratio = 1: 3: 1) 51 Example 3 Seisin, Taeunggung, Chungbuk mixed extract
(Mixing ratio = 1: 1: 3) 50 Example 4 Seisin, Taeunggung, Chungbuk mixed extract
(Mixing ratio = 1: 3: 3) 48

As can be seen in Table 1, Examples 1 to 4 of the mixed extract of Seixin, Tongkung and Chinin according to the present invention exhibited better activity than the single extract of the comparative example and showed antioxidant ability similar to Trolox, an antioxidant known in the art I could confirm.

[ Test Example  2] Measurement of inhibitory effect of melanin formation by mouse pigment cells

In order to examine the inhibitory effect of the extracts of the comparative examples and the examples and the control sample on melanin production, rat pigment cells were used.

First, the mouse melanoma cells (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) derived from C57BL / 6 mice were inoculated into DMEM with 10% fetal bovine serum, 100 nM 12- The cells were cultured in a medium supplemented with 12-O-tetradecanoylphorbol-13-acetate and 1 nM cholera toxin at 37 ° C and 5% CO ₂ . The cultured Mel-Ab cells were removed with 0.25% trypsin-EDTA, the cells were cultured at a concentration of 10 ⁵ cells / well in a 24-well plate, and 10 ppm of each test substance Hydroquinone, the above-mentioned Comparative Examples and the extracts of Examples were added to a control sample and cultured. At this time, the hydroquinone was used as a positive control. Then, the culture solution was removed, washed with PBS, and the cells were dissolved with 1 N sodium hydroxide, and the absorbance at 400 nm was measured. The rate of inhibition of melanin production was calculated according to the following formula (1) Method).

Test substance Melanin production inhibition rate (%) Comparative Example 1 (10 ppm) 12.5 Comparative Example 2 (10 ppm) 24.8 Comparative Example 3 (10 ppm) 23.1 Example 1 (10 ppm) 49.5 Example 2 (10 ppm) 51.4 Example 3 (10 ppm) 50.5 Example 4 (10 ppm) 52.0 Hydroquinone (control group) 50.8 Control group 0

As shown in the above Table 2, Examples 1 to 4 of the mixed extract of Seixin, Tongkung and Chinju obtained by the present invention showed better melanin production inhibitory rate than the single extract of Comparative Example. Especially, the mixed extracts showed hydroquinone And a similar degree of inhibition of melanin production was observed.

Claims (6)

A cosmetic composition for skin whitening comprising as an active ingredient a mixture of sesquin extract, cinnabar extract, and goat extract,
The cosmetic composition for skin whitening according to claim 1, wherein the mixture is a mixture of sesquin extract, cinnabar extract, and callus extract in a ratio of 1: 1 to 3: 1 to 3 by weight.
The cosmetic composition for skin whitening according to claim 1, wherein the content of the mixture is 0.001 to 20% by weight based on the total weight of the composition.
delete The composition according to claim 1, wherein the mixture is a mixture of sesamin, cinnabar, and goat extract in a weight ratio of 1: 1: 1, 1: 3: 1, 1: 1: 3, or 1: 3: 3 Wherein the cosmetic composition is a cosmetic composition for skin whitening.
[Claim 5] The cosmetic composition for skin whitening according to claim 4, wherein the mixture is a mixture of sesqui extract, cinnabar extract, and passerine extract in a weight ratio of 1: 3: 3.
The cosmetic composition for skin whitening according to claim 2, wherein the content of the mixture is 0.005 to 10% by weight based on the total weight of the composition.