KR100839704B1 - Hair restorer and hair growth promoter composition - Google Patents

Abstract

A composition for promoting hair growth and restoration comprising a non-polar organic solvent fraction of Chrysanthemum zawadskii var. latilobum is provided to promote the hair growth by shortening a period between interphase and a growth period of air without side effects. A composition for promoting hair growth and restoration comprises an organic solvent fraction of Chrysanthemum zawadskii var. latilobum as an effective ingredient, wherein the organic solvent fraction is a second extract fraction prepared by extracting the Chrysanthemum zawadskii var. latilobum with a first extraction solvent and then extracting the first extract with a second extraction, wherein the first extraction solvent is at least one solvent selected from the group consisting of purified water, a lower alcohol such as methanol, ethanol, isopropyl alcohol, and n-butanol, a polyhydric alcohol such as glycerol, ethylene glycol, propylene glycol, and 1,3-butylene glycol, and a hydrocarbon-based solvent such as methyl acetate, ethyl acetate, benzene, n-hexane, diethylether, dichloromethane and chloroform and the second extraction solvent is at least one selected from the group consisting of petroleum ether, methylacetate, benzene, hexane, chloroform. ethylene chloride, dimethyl ether, and ethyl acetate. A method for preparing the composition comprises the steps of: (a) washing and finely cutting stems and leaves of the Chrysanthemum zawadskii var. latilobum; (b) precipitating the cut Chrysanthemum zawadskii var. latilobum in the first extraction solvent for 1-10 days at a temperature of 18-27 deg.C; (c) filtering, concentrating and freeze-drying the first extract to obtain a dried extract of the Chrysanthemum zawadskii var. latilobum; (d) dissolving the dried extract in water; and (e) extracting the aqueous solution of the Chrysanthemum zawadskii var. latilobum with at the second extraction solvent. A hair cosmetic composition such as shampoo, rinse, ample and treatment comprises the composition for promoting hair growth and restoration. Further, the composition for promoting hair growth and restoration is one selected from liquid agent for external use, cream, ointment, paste, aerosol agent, gel and wax.

Description

Hair restorer and hair growth promoter composition

Figure 1 schematically shows a method of fractionating Gujeolcho extract according to the organic solvent polarity,

Figure 2 is a graph showing the results of the hair growth test using the mouse C57BL / 6 using the organic solvent fraction of Gujeolcho extract obtained according to Figure 1,

3 is an animal photograph showing the result of FIG. 2.

The present invention relates to a hair growth and hair growth composition containing an organic solvent fraction of Guryecho (Chrysanthemum zawadskii var.latilobum) extract as an active ingredient, and a method for preparing the same, and particularly to a nonpolar organic solvent fraction of Gujeolcho which is a natural plant. The present invention relates to a hair growth and hair growth composition, which has been proved experimentally with excellent effects without any side effects, including as an active ingredient.

Hair loss is a major cause of genetic factors, and stress is also a major cause of hair loss. Recently, hair loss population is gradually increasing due to the increase of social stress, westernized eating habits such as environmental pollution and instant food, frequent perming and dyeing, and poor scalp management.

Hair is maintained by repeating hair growth and hair loss in cycles of anagen, catagen, and telogen. Specifically, anagen, which grows hair, catagen, which is the end of growth and shrinking of the hair follicles, talogen, which is the time when the nipple stops working and the hair stays in the scalp. It can be divided into a generator, which is a time to start or to dehair old hair by generating new hair.

The hair grows naturally for men 3 to 5 years, for women 4 to 6 years, then for 30 to 45 days of degeneration, and for 3 to 4 months. At the end of the off-season, the generator starts producing new hair.

Hair loss is a normal phenomenon, but a normal person has a lot of hair in the growth phase, while alopecia usually has a lot of hair in the resting phase, which causes visible hair loss.

For the first time, hair follicle formation occurs when placode is formed by the action of beta-catenin (Laura C. 2003). Hair is produced by dermal papilla cells. This period of hair growth is called growth. The first hair produced passes through the growth phase and enters the resting phase, which passes through the degenerative phase of hair loss and then enters the growth phase again.

The growth stage (Anagen Stage; 2-7 years) is the period of hair growth. The growth stage is further divided into two stages. This is the stage where hair is produced from the hair follicles to the follicles and hard keratin is produced inside the hair follicles. Continue self-growth until retrogression. The growth period is 2-7 years, accounting for 89-90% of all hairs (10-150,000), and the hair grows 1-1.5cm per month at the growth stage. May be altered by the effects of food, hormones, vitamins.

The Cataract Stage (2 to 3 weeks) grows slowly as the metabolic process slows down, while the growth phase ends. This step does not produce keratin. The degenerative lifespan is 2 to 3 weeks and accounts for 1% of all hair. The capillary part contracts and is divided into the breast papilla, which is surrounded by the hair follicle and rises upward. At this time, cell division is stopped.

The Talogen Stage (3 months) contracts the dermal papilla, the hair follicles crumble gradually, and the hair roots push upwards and fall out. The period of hair loss is 3-4 months until the next growth stage begins. 4-14% of the total hair (30-40% after delivery) and the hair at this time is strong brushing, hair is easily lost.

Alopecia is roughly classified into androgenetic alopecia, female pattern hair loss, alopecia areata, and other causes of alopecia.

Androgenetic alopecia develops male sexual characteristics and is caused by a male hormone called testosterone, a hormone that acts on the development of muscles and the development of male organs during puberty, and this testosterone is called 5 alpha-reductase. When the enzyme is changed to Dihydrotestosterone (DHT), a more powerful hormone, it acts on the dermal papilla cells in hair follicles, leading hair follicles from the growth stage to the degenerative stage, causing hair loss.

Gynecologic alopecia is mainly caused by a decrease in the amount of estosterone after menopause. Female alopecia, unlike the appearance of male alopecia, does not fall out of the front of the head, only the hair in the middle part is mainly lost. Alopecia in women has a lower association with 5 alpha-reductase than men. Thus, drugs that inhibit 5 alpha-reductase have little effect on postmenopausal alopecia women.

Alopecia areata is caused by autoimmune diseases, mental stress, or genetic predisposition. Hair loss of round or oval shape occurs, and it is characterized by ringworm whitening or hair growth wall. Alopecia areata is fundamentally different from androgenetic alopecia, and the treatment is also different, so that a method of treating corticosteroids is applied or minoxidil is applied to the affected area.

In regard to such diverse and complex hair loss causes, hair loss prevention products known to date are available as commercially available ingredients for promoting blood circulation, inhibiting testosterone action, and strengthening hair root function. There is no clear effect yet, and side effects may be raised.

These hair regrowth agents are initially developed and used to promote blood circulation, and are known to exhibit hair growth effects as a side effect among patients using them, and subsequently approved by the US Food and Drug Administration (FDA) as a raw material for hair growth. Minoxidil (6-Amino-1,2-dihydro-1-hydroxy-2-imino-4-phenoxypyrimidine) of U.S. Patent No. 3,382,247, which has been used to treat hair growth, is still being used as a therapeutic agent for hair growth. Merck's finasteride, referred to in US Pat. No. 5,215,894, was originally used as a prostate treatment for men, but was also used as a treatment for androgenetic alopecia by controlling its dosage in the body. However, it is currently used as an oral preparation, but side effects such as sexual dysfunction have been reported according to its intake, and it is effective only when a steady dose is taken for hair loss, and their 5 alpha-reducta It is difficult to anticipate the effect of an azeotropic activity by application | coating, and it can expect an effect only by oral administration, and there exists much inconvenience in use.

In addition, Korean Patent Application No. 10-2005-54529 discloses a cosmetic for the purpose of anti-inflammatory and blood flow improvement using gujeolcho, and Korean Patent Application No. 10-2004-58595 extracts ginseng and gujeolcho from the solvent to use as a hair growth ingredient Although there is an example, the patent of 10-2005-54529 did not recognize the hair growth effect of gujeolcho, and the patent of 10-2004-58595 simply mixed several herbal medicines and added water to add water to extract the effect. It is difficult to confirm whether the effect of Gujeolcho, and simply as an extract of water, the hair growth effect is insufficient enough to use industrially.

In view of the above problems, the present inventors were screening dozens of plant extracts having hair growth promoting effects to verify the effects on various hair loss mechanisms, while the organic solvent fraction extract of Gujeolcho The present invention has been completed by discovering that hair is newly grown at a rapid rate by shortening the period of the transition to the growth phase, thereby having a hair growth promoting effect.

It is an object of the present invention to provide a hair growth and hair growth composition having excellent hair growth and hair growth effects without any side effects.

Still another object of the present invention is to provide a method for producing a hair growth and hair growth composition having excellent hair growth and hair growth effects and a simple manufacturing process.

In order to achieve the above object, the present invention provides a hair growth and wool composition comprising the organic solvent fraction of Gujeolcho as an active ingredient.

The organic solvent fraction is preferably a nonpolar organic solvent fraction, particularly an organic solvent fraction having a polarity of ethyl acetate or less.

The organic solvent fraction according to the present invention can also be used as an organic solvent extraction fraction extracted directly from Gujeolcho, the second extraction fraction which is extracted first by extracting Gujeolcho as an extraction solvent, and extracted by extracting the primary extract again with an organic solvent. Do.

Extraction solvents used for the preparation of the primary extract of Gujeolcho is not particularly limited in its selection is purified water; Lower alcohols such as methanol, ethanol, isopropyl alcohol and n-butanol; Polyhydric alcohols such as glycerol, ethylene glycol, propylene glycol and 1,3-butylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane, chloroform and the like.

According to one embodiment of the present invention, purified water and lower alcohols such as methanol, ethanol, and n-butanol were used as extraction solvents in the primary extraction solvent, and one or two or more kinds of solvents may be used. According to the present invention, lower alcohols such as 50 to 100% ethanol can be used as the primary extraction solvent.

The secondary extract fraction according to the present invention is at least one non-polar organic solvent selected from the group consisting of petroleum ether, methyl acetate, benzene, hexane, chloroform, methylene chloride, dimethyl ether, ethyl acetate, that is, organic having an ethyl acetate or less polarity. Solvents are preferred. Therefore, all extracts containing the components of the fraction extracted with Gujeolcho with an organic solvent having a polarity of less than ethyl acetate may be included in the context of the present invention.

Gujeolcho organic solvent fraction according to the present invention is preferably included in the form of soft x to 0.0001 to 30 parts by weight, preferably 0.01 to 10.0 parts by weight based on 100 parts by weight of the entire hair regrowth composition. Less than 0.0001 parts by weight of the hair growth induction promoting effect does not appear, the concentration is higher than 30 parts by weight is uncomfortable compared to the effect that appears difficult to apply is not preferable.

The composition of the present invention may contain a variety of plant extracts that are effective for hair growth and hair growth with Gujeolcho extract, and one or two ingredients effective for hair growth and hair growth, which are stored in the process such as the Korean Pharmacopoeia or herbal medicine standard collection It may include more.

For example, nasturtium extract, white porcelain extract, clove extract, ginger extract, birch extract, green tea extract, uiyiin extract, red sesame extract, schisandra extract, cabbage extract, leaflet extract, peony extract, red peony extract, fennel extract, etc. But it is not limited thereto. The plant extracts can be used extracted in a general manner known in the art.

In addition, the composition of the present invention, in consideration of the properties of the hair, may be added to the auxiliary components such as anti-dandruff, hair root enhancer, hair repair, scalp protector, refreshing agent for additional function.

Auxiliary components, such as a preventive agent, a hair root strengthening agent, a hair repair agent, a scalp protector, and a refreshing agent, blended in these conventional hair growth and hair growth compositions can be used, but is not limited thereto.

These auxiliary ingredients can be used within the scope for maintaining the formulation without disturbing the effects of the main ingredient.

Formulations in which the hair growth and hair growth compositions of the present invention can be used include topical application forms, shampoos, rinses and ampoules, which can be applied to the scalp such as external preparations, creams, ointments, pastes, aerosols, gels and waxes. All formulations applied to the hair, such as treatments, may be included.

For another object of the present invention, the present invention

Purifying and slicing stem and leaf portions of gujeolcho;

Chilling at 18-27 ° C. for 1-10 days in a primary extraction solvent of about 4-10 times the pulverulent pulverulent;

Obtaining the Gujeolcho dry extract through the primary extract of Gujeolcho by filtration, concentration and lyophilization;

Dispersing and dissolving the Gujeolcho dry extract in water; And

It provides a method for producing a hair growth and hair growth composition comprising the step of extracting and extracting the aqueous solution of Gujeolcho with an organic solvent that is a secondary extraction solvent.

The primary extraction solvent is not particularly limited in its selection is purified water; Lower alcohols such as methanol, ethanol, isopropyl alcohol and n-butanol; Polyhydric alcohols such as glycerol, ethylene glycol, propylene glycol and 1,3-butylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane, chloroform and the like.

According to one embodiment of the present invention, purified water and lower alcohols such as methanol, ethanol, and n-butanol were used as extraction solvents in the primary extraction solvent, and one or two or more kinds of solvents may be used. According to the present invention, lower alcohols such as 50 to 100% ethanol can be used as the primary extraction solvent.

The secondary extraction solvent is preferably a nonpolar organic solvent, preferably an organic solvent having a polarity of ethyl acetate or less. For example, solvents frequently used when extracting natural products include petroleum ether <hexane <benzene <chloroform <methylene chloride <ether <ethyl acetate <acetone <butanol <isopropanol <ethanol <methanol <water depending on the polarity And organic solvents having a polarity of less than double ethyl acetate are preferred. Therefore, all extracts containing the components of the fraction extracted with Gujeolcho with an organic solvent having a polarity of less than ethyl acetate may be included in the context of the present invention.

For another object of the present invention, the present invention is also possible to omit the extraction step of the lower alcohol of the first extraction step and extract with an organic solvent having a polar strength of less than the ethyl acetate of the second extraction step, such a All extracts including components of the extracts may also be included in the context of the present invention.

Gujeolcho (九 節 草, Chrysanthemum zawadskii var.latilobum), the main constituent of the present invention, is a representative wild flower blooming in autumn, and stems become 5 nodes on May Dano, and 9 nodes on September 9 (lunar calendar). It is called Gujeolcho. In addition, Gujeolcho has a single flower at the end of the stem, which is called Seonmocho because white flowers stand out more than fresh.

The Gujeolcho is native to the whole country except the beach and grows in a colony, especially in high and deep mountains. Gujeolcho is a single species (種 一種), but there are several varieties. Sangujeolcho is 10 ~ 60 ㎝ high and 3 ~ 6 ㎝ in flower diameter. Rock Gujeolcho is a Korean native plant that grows in deep mountains in the mid-Northern region. It is 20 ㎝ high and 2 ~ 4 ㎝ in flower diameter. Pocheon Gujeolcho is native to the Hantan River and has pink flowers. Gujeolcho, one of our native horticultural flowers, is a perennial grass of the Asteraceae family. So it is our flower that can compete with cosmos which is a foreign plant in the fall. Gujeolcho in oriental medicine is effective for women's diseases and thermal insulation. In particular, it is effective for menstrual disorders, and when you go to the medicinal market, gujeolcho is enough. Flowers can be soaked or dried in pillows to help with headaches and hair loss, and to prevent whitening of hair. Young shoots are eaten with herbs and leaves are fragrant when put on rice cakes.

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples. However, the following Examples and Comparative Examples are only examples, but are not limited thereto.

Example  1: Gujeolcho organic solvent Fraction  Produce

First, the stems and leaves of gujeolcho were sieved to filter out impurities, and then ethanol and purified water were added in about 4 to 10 times according to the standards set forth in the Korean Pharmacopoeia, followed by cooling at room temperature for 5 days. The extracted stock solution was filtered using Whatman # 3 filter paper, and the filtered extract was completely evaporated at 45 ° C. using a vacuum condenser to prepare a dried extract.

The prepared extract was dispersed / dissolved in water again to obtain fractions according to polarity step by step using three organic solvents of hexane, chloroform and ethyl acetate (see FIG. 1).

Experimental Example  1: mouse C57BL Of 6  Used in vivo Hair growth  exam

Experimental methods mainly used to study hair growth and hair growth effects are mainly used in vivo evaluation using experimental animals and in vitro evaluation using tissue culture of dermal papilla cells. The research and evaluation methods related to hair growth and hair growth using experimental techniques have been actively conducted.

In the present invention, each fraction obtained in accordance with Example 1 was used for in vivo hair growth test using mouse C57BL / 6.

C57BL / 6 mice are hairy degenerative from 6 weeks of age, go to rest from 7 weeks of age, and return to growth at 12 weeks of age. For this reason, it is the most in vivo animal model used for hair growth test.

Six-week-old mouse C57BL / 6 females were purchased with D.O.B. (day of birth) selected as subjects weighing less than three days. Experiments were carried out at 7 weeks of age with adaptation for 1 week at 25 ° C and 50% humidity.

The mouse was placed in a desiccator containing ether and anesthetized for 1 minute, and then the hair of the mouse was removed using a hair remover. After 1 day, a sample was applied to the uninjured mouse.

Samples were coated 100ul once a day, and photographed at two-day intervals. The color change of the coated area was quantified using an image analyzer.

Each sample was dissolved in 70% ethanol and used for the experiment. The negative control group was coated with only 70% ethanol alone. As a positive control group, minoxidil 3%, which is a widely used medicine as a hair regrowth agent, was used. Each sample was dissolved at a concentration of 5% and used for the experiment. The results are shown in FIGS. 2 and 3, respectively.

As shown in the results of FIGS. 2 and 3, the hexane extraction fraction showed the highest hair growth activity, and the chloroform fraction also showed some activity. From the above results, it could be inferred that the hexane fraction and the chloroform fraction in the gujeolcho extract contained components promoting hair growth. It was also inferred that the residual water soluble components after organic solvent extraction had no hair growth effect.

After 6 weeks of age, hairs become degenerative, and from 7 weeks of age to dormant, and at 12 weeks of age, C57BL / 6 mice having a cyclic characteristic of returning to the growth phase were tested as described above. During this growth cycle, by confirming that the period of transition from the resting phase to the growing phase is shortened by two to three weeks, the composition according to the present invention shortens the step of transitioning from the resting phase of the hair to the growing phase, thereby causing the hair to grow rapidly at a new speed. It was confirmed to have a growth promoting effect.

Experimental Example 2: Evaluation of the activity of hair follicle cells

Cell lines were obtained from human dermal papilla cells purchased from SCIENCELL.

Cell culture was used as a culture medium in mesenchymal cell medium and cultured in 37 ℃, 5% CO ₂ incubator.

MTT assay was performed to determine the effect of promoting cell growth. MTT assay was performed in the following manner.

First, human dermal papilla cells pre-cultured at 100 dishes were removed using trypsin, and the cells were counted using a hemocytometer to be 105 cells / ml.

100 ul (104 cells per Well) of the cell solution was placed in a 96 well plate and incubated for 1 day.

After 1 day of incubation, each test sample was diluted to 2% in mesenchymal cell medium, and then 100 ul of the sample solution was diluted to 1/2 by serial dilution in 18 wells. Diluted until.

Each sample solution was added to 96 wells containing cells cultured for 1 day so that the first stage concentration of each sample was 1%.

96 wells of the samples were incubated for 1 day at 37 ° C. and 5% CO 2, followed by 0.1 mg (20 μl of 5 mg / ml) of MTT (3- (4,5-dimethylthiazol-2-yl) -2, 5 -diphenyl tetrazolium bromide) solution was added to all wells. After further incubation at 37 ° C. for 4 hours, the plate was centrifuged at 1000 rpm for 5 minutes to remove all of the medium. 200 μl of DMSO was added to each well to allow the resulting formazan salt to dissolve completely. After measuring absorbance at 570 nm with a microplate reader, the extent of growth of the cells was determined.

The average OD570 value of each well column is obtained to calculate a percentage value of the average OD570 value of the control group. This percentage corresponds to the cell viability of the test group compared to the control. The results are shown in Table 1.

Gujeolcho Hexane Extract concentration Control 0.00001% 0.0001% 0.001% Cell activity (%) 100 112 128 132

As shown in the above results, gujeolcho extract seems to activate the hair growth field by activating the growth of dermal papilla cells.

Example  2 to 4

A hair growth composition was prepared using the Gujeolcho hexane fraction prepared according to Example 1 in the configuration shown in Table 2 below.

Comparative example  1 and Comparative example  2

Comparative Example 1 and Comparative Example 2 were prepared using the gujeolcho organic solvent fraction according to the present invention as shown in Table 2, and the composition was prepared using the water-soluble residual fraction.

Ingredients (% by weight) Comparative Example 1 Comparative Example 2 Example 2 Example 3 Example 4 ethanol  40 40 40 40 40 1,3-butylene glycol  10 10 10 10 10 Gujeolcho hexane fraction  0  0 0.1 5 10 Gujeolcho water-soluble residual fraction  0  10 0 0 0 Purified water Residual amount Residual amount Residual amount Residual amount Residual amount

Experimental Example  3: Clinical Trials in Hair Loss Patients

In order to evaluate the effects on alopecia patients, a clinical trial was conducted in patients with alopecia.

Subjects of male and female alopecia among adult men and women aged 20 years or older were sprayed 2 to 3 times a day to the hair loss site for each sample, from 2 months to 4 months. It was.

Judgment of the effect was evaluated in five stages according to the following evaluation scales of the photographic findings and the improvement of subjective symptoms (Table 3). The results are shown in Table 4.

step Visual judgment Subjective symptoms One Got worse Got worse 2 No change No change 3 Slightly improved Slightly improved 4 Improvements were made. Improvements were made. 5 Significantly improved Significantly improved

division Visual judgment Subjective symptoms Comparative Example 1 2 2.2 Comparative Example 2 2 2.1 Example 2 2.8 3.0 Example 3 3.8 4.2 Example 4 4.1 4.5

As shown in the results of Table 4, it was found that Examples 2, 3, and 4, including the hexane fraction of Gujeolcho, showed excellent effects, and were excellent in preventing hair loss and hair regrowth. In Comparative Example 2, there was no effect.

In addition, no adverse effects were found in all subjects during the trial period, indicating that they are safe for humans.

Taken together, the Gujeolcho organic solvent fraction showed an effect of promoting hair growth phase in the animal experiment model, and also showed excellent results in the activity evaluation experiment using hair follicle cells. Very good effect.

Thus, it can be seen that Gujeolcho organic solvent fractions exhibit hair growth and hair growth effects.

Example  5 to 7

Hereinafter, the composition of the hair and scalp application composition of various forms containing the Gujeolcho hexane fraction obtained according to Example 1 of the present invention is shown in Tables 5 to 8.

shampoo Ingredients (% by weight) Example 5 Sodium Lauryl Sulfate 10 Cocamidopropyl Betaine 3 Carboxyl Vinyl Polymer 0.3  Polyquaternium-10 0.2 incense 0.7 Methylparaben 0.2 Cetyltrimethylammonium chloride 0.1 Gujeolcho Extract 5 Purified water Residual amount

Rinse Ingredients (% by weight) Example 6 Cetyl alcohol 2 Stearyl alcohol 2.5 Behenyl Alcohol 0.5 Spices 0.4 Silicone emulsion 0.4 Cyclomethicone 1.0 Dimethyl distearyl ammonium chloride 0.1 Citric acid Adjustable amount to pH 3-7 Gujeolcho Extract One Purified water Residual amount

ample Ingredients (% by weight) Example 7 ethanol 30 Propylene glycol 20 1.3-butylene glycol 10 Cetyltrimethylammonium chloride 3 Dimethyl distearyl ammonium chloride 0.1 Lauryl Alcohol 3 Myristyl Alcohol 5 Cetyl alcohol One incense 0.5 dyes A reasonable amount Gujeolcho Extract One Purified water Residual amount

According to the present invention, the effect of Gujeolcho organic solvent fractions exhibiting hair growth and hair growth through hair growth promotion was confirmed, and the cycle of transition from the resting phase to the growing phase of the hair growth cycle leading to the growth phase, the degeneration phase, and the resting phase It has been shown that the growth promoting effect of hair, which causes hair to grow rapidly by shortening, is excellent.

In addition, the composition of the present invention has almost no side effects, and by selecting and using only fractions having excellent hair growth and hair growth effects among gujeolcho extracts, blocking the possibility of containing various components, and exhibits more excellent hair growth and hair growth action.

Claims (10)

Organic solvent fraction of gujeolcho as an active ingredient, The organic solvent fraction is a second extraction fraction which is extracted again the first extract extracted the Gujeolcho with the first extraction solvent, The primary extraction solvent is purified water; Lower alcohols such as methanol, ethanol, isopropyl alcohol or n-butanol; Polyhydric alcohols of glycerol, ethylene glycol, propylene glycol or 1,3-butylene glycol; And one or two or more kinds of mixed solvents selected from hydrocarbon solvents of methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane or chloroform, The secondary extract fraction is a hair growth and wool composition, characterized in that the fraction of at least one organic solvent selected from the group consisting of petroleum ether, methyl acetate, benzene, hexane, chloroform, methylene chloride, dimethyl ether and ethyl acetate. delete delete delete The hair growth and wool composition according to claim 1, wherein the Gujeolcho organic solvent fraction is contained in an amount of 0.0001 to 30 parts by weight based on 100 parts by weight of the total hair growth composition. The hair growth and wool composition according to claim 5, wherein the Gujeolcho organic solvent fraction is contained in an amount of 0.01 to 10 parts by weight. delete Purifying and slicing stem and leaf portions of gujeolcho; Purified water which is about 4 to 10 times the primary extraction solvent of the Gujeolcho crushed product; Lower alcohols such as methanol, ethanol, isopropyl alcohol and n-butanol; Polyhydric alcohols of glycerol, ethylene glycol, propylene glycol and 1,3-butylene glycol; And in the one or two or more kinds of mixed solvents selected from hydrocarbon solvents of methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane, chloroform, cold immersion at 18 ~ 27 ℃ for 1 to 10 days; Obtaining the Gujeolcho dry extract through the primary extract of Gujeolcho by filtration, concentration and lyophilization; Dissolving the Gujeolcho dry extract in water; And And extracting and extracting the Gujeolcho aqueous solution into at least one organic solvent selected from the group consisting of petroleum ether, methyl acetate, benzene, hexane, chloroform, methylene chloride, dimethyl ether, and ethyl acetate. Hair growth and hair growth composition manufacturing method. At least one scalp topically applied hair regrowth agent selected from external solutions, creams, ointments, pastes, aerosols, gels and waxes comprising the hair growth and hair growth composition of any one of claims 1, 5 and 6. . At least one hair cosmetic composition selected from shampoos, rinses, ampoules, or treatments comprising the hair growth and hair growth compositions of any one of claims 1, 5 and 6.

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