KR101114307B1 - Hair growth agent composition - Google Patents

Abstract

The present invention relates to a hair loss prevention and hair growth accelerator composition containing Adenophora triphylla extract, which shortens the transition period from the resting phase to the growth phase of the hair growth cycle as an active ingredient, thereby allowing new hair to grow quickly. The composition of the present invention containing ginseng extract having excellent hair follicle cell activity with a hair growth induction promoting effect has little side effects, shows excellent hair growth promoting effect and prevents hair loss, and simultaneously contains a component having a 5α-reductase activity inhibitory effect. It is very safe even when contained, and has an excellent hair loss prevention and hair growth promoting effect.

Ginseng extract, 5 alpha-reductase, promote hair growth, hair follicle activity, hair loss prevention.

Description

Cosmetic composition having hair loss prevention and hair growth promoting effect {HAIR GROWTH AGENT COMPOSITION}

The present invention relates to a hair loss prevention and hair growth promoter composition containing the extract Asam ( adenophora triphylla ), more specifically, the present invention is to reduce the period of transition from the resting phase to the growth phase of the hair growth cycle as an active ingredient The present invention relates to a hair growth promoter containing four ginseng extracts excellent in hair follicle cell activity, along with a hair growth phase induction promoting effect that allows new hair to grow faster.

Human hair has about 100,000 to 150,000 pieces, and each hair has a different cycle and grows and falls through the anagen, the catagen, and the telogen. This cycle is repeated over three to six years, resulting in an average of 50 to 100 hairs falling out per day. Generally, the term alopecia refers to the increase in the number of hairs that fall abnormally due to shortening of the hair growth rate in the growth phase and more hair in the degenerative or resting phase.

The causes of alopecia have been discussed, such as excessive male hormone action, excessive sebum secretion, poor blood circulation, hypoglycemia caused by peroxide or bacteria, genetic factors, aging, stress. However, to date, no clear cause for hair loss is unknown, and it is known that a very complicated action is intertwined.

As for the various causes of hair loss, hair loss prevention products known to date are effective ingredients for promoting blood circulation, strengthening hair root function, moisturizing scalp, preventing dandruff, antioxidant effect, hair growth period, and suppressing male hormone activity. It contains ingredients, etc., but there is still no clear effect in terms of effects and side effects may be raised.

Experimental methods used to prevent hair loss and promote hair growth are mainly used in vivo evaluation using experimental animals and in vitro evaluation using hair follicle cells and tissue culture. In the 1980s, research on the differentiation mechanism of hair follicles became active with the development of culture methods of dermal papilla cells and outer root sheath cells, which are the core cells of hair follicles. With the development of tissue culture methods, it has been shown to be a useful evaluation model for research on hair growth, wool and hair loss prevention. In recent years, biochemical and molecular biological experiments have been actively conducted to identify various growth factors and genes related to hair growth, research on mechanism of action, and drug search.

The effects of a commercially available product on the hair as a hair growth or hair loss preventing agent on the hair include growth phase induction effect, hair growth phase extension effect, 5α-reductase inhibitory effect, blood circulation promoting effect, bactericidal effect, anti dandruff effect, moisturizing effect, antioxidant effect, etc. However, in the existing formulations, hair loss prevention and hair growth promoting effects are not sufficient.

Androgenetic alopecia is directly dependent on the amount of androgen, which is dependent on androgen hormones. Accordingly, many studies have recently been reported to prevent and treat hair loss by inhibiting androgen activity. Briefly explain the mechanism of hair loss on male hormones are as follows. In other words, testosterone, one of the male hormones, is converted to dihydrotestosterone, an active male hormone, by an enzyme called 5α-reductase, and this activated hormone binds to a specific receptor and causes a hair loss protein. Induces hair loss. In addition, such mechanisms may cause excessive production of sebum, causing acne, seborrheic dermatitis, and the like, and as a result, scalp may occur with inflammation. As a result, androgenetic alopecia is due to the excessive production of dihydrotestosterone by the action of 5α-reductase, it will be able to prevent and treat masculine alopecia by inhibiting the activity of 5α-reductase. In view of this, male hair loss treatment using finasteride, which has been used as a prostate treatment for men, has been developed, but side effects such as sexual dysfunction have been reported, and their 5α-reductase activity against hair loss It is also difficult to fully anticipate the effect by inhibitory effect alone.

In view of the above problems, the present inventors screened dozens of plant extracts through animal experiments to confirm the growth-induction promoting action of hair among the effects on various mechanisms, hair growth induction-promoting effect in the three ginseng extract It was found to be excellent, and also in the experiment evaluating the hair follicle cell activity of the ginseng extract, very good results were obtained, which led to the completion of the present invention.                         

Specifically, the present inventors selected dozens of plant extracts having the effects of preventing hair loss and promoting hair growth for alopecia and securing safety to the human body, and conducted a study on hair growth induction promoting effect. In the hair growth cycle where new hair grows, grows and falls out, it is found that it promotes the transition from the resting phase to the growth phase. Moreover, Samsam extract showed very good results in vitro evaluation of the activity of hair follicle cells.

An example of using such ginseng extract in hair products is Japanese Patent Laid-Open Publication No. 1991-176414, which discloses a hair conditioning composition by anti-dandruff action using Adenophora stricta , which is a similar plant of ginseng. However, there is no document that describes using ginseng in connection with promoting hair growth or preventing hair loss.

It is an object of the present invention to provide a hair growth promoter composition which has little side effects and has excellent hair growth promoting and anti-hair loss effects.

In order to achieve the above object, the present invention provides a hair loss prevention and hair growth promoter composition comprising four extracts (沙 蔘, Adenophora triphylla ) as an active ingredient.

Here, the extraction solvent of the ginseng extract may be one kind or a mixture of two or more kinds of lower alcohols, and the content of the ginseng extract is preferably 0.01 to 10% by weight of the total weight of the composition as a solid content.

In addition, the composition of the present invention may further comprise at least one 5α-reductase activity inhibiting component, wherein the content of the 5α-reductase activity inhibiting component is preferably 0.001 to 10% by weight.

In addition, the composition of the present invention may be included in a transdermal topical formulation that can be applied to the scalp, such as a liquid, cream, paste, aerosol.

The composition comprising ginseng extract according to the present invention, the ginseng extract not only has a clear mechanism of action of promoting hair growth phase induction, but also has an active action on hair follicle cells, thereby exhibiting hair growth promoting effect. In addition, when the ginseng extract further comprises 5α-reductase activity inhibiting ingredient as an active ingredient, hair loss prevention and hair growth promoting effect may be more excellent.

Hereinafter, the composition of this invention is demonstrated more concretely.

First, in view of the background of the present invention, in order to prevent hair loss and promote hair growth for alopecia, various hair loss mechanisms or mechanisms of action should be approached through a variety of mechanisms. The study was examined. First of all, we have screened hundreds of combinations of male hormone-regulating components, blood circulation promoting components, cell activating components, and anti-inflammatory components, and hair loss prevention and hair growth promoting effects. As a result, it was found that Samsam extract has excellent hair growth induction promoting action and hair follicle cell activity. In addition, it was found that the composition including the 5α-reductase activity inhibitory ingredient for controlling male hormone action together with Samsam extract has not only a very good hair loss prevention and hair growth promoting effect, but also no side effects. Therefore, in the present invention, in terms of hair growth stage induction promoting action, hair follicle cell activation action, 5α-reductase activity inhibiting action, and the like, which are evaluated as important factors for hair growth and hair loss prevention, a combination of various causes of hair loss is provided. It is to provide a composition having a hair loss prevention and hair growth promoting action.

In the composition of the present invention, Samsam is a herbal medicine using the roots of Adenophora triphylla or other similar plants (campanula and Campanulaceae), and the herbal name is Adenophorae Radix. The shape of samsam is bent into fusiform to long cone shape and rarely has branch root. Root stems with limbal folds in upper part, 5 ~ 20 ㎝ long, 1 ~ 3 ㎝ in diameter above root. The outer surface of the root is light yellowish white to light grayish brown, and the upper part has distinct circumferential wrinkles and the lower part has vertical and lateral wrinkles. The root is light and easy to bend, and the cut side is milky white with many gaps. This herbal medicine has a unique aroma, tastes a little sweet, and chews mucus. Representative active ingredients include saponin, inulin, phytoderin, phytoderin, leoithin, pentosan, and other active ingredients. Medicinal has been used in tonic, fever, expectorant, detoxification, sore throat, lymphadenitis, boil treatment.

Looking at the production method of the four ginseng extract contained in the composition of the present invention, first purified and shredded roots of four ginseng, cooled for 5 days in about 10 times the amount of the extraction solvent, and then filtered the extraction stock solution, concentration and lyophilization method Through dried ginseng extract to obtain a powder. At this time, the extraction solvent is not particularly limited in the selection, purified water; Lower alcohols such as methanol, ethanol, isopropyl alcohol and n-butanol; Polyhydric alcohols such as glycerol, propylene glycol and 1,3-butylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane, and the like. Among these solvents, lower alcohols such as methanol, ethanol and n-butanol are preferably used as extraction solvents, and one or two or more kinds of these solvents may be mixed and used for extraction.

The ginseng extract prepared as described above is preferably included as a solid content in 0.01 to 10% by weight of the total weight of the composition of the present invention. If it is less than 0.01% by weight, the hair growth phase induction promoting effect is not satisfactory, and at a concentration higher than 10% by weight, the effect thereof is not sufficient compared to the dose, which is not preferable.

The 5α-reductase activity inhibiting component which may be included with the extract of Samsam in the composition of the present invention is a compounding ingredient of an external preparation that exhibits a hair loss prevention effect by inhibiting the action of 5α-reductase in human hair follicles, and has no problem in terms of safety. It is not specifically limited, It can mix | blend individually or in combination of 2 or more components. Such 5α-reductase activity inhibitory ingredients include, for example, ginseng extract, uiyiin extract, clove extract, Bupyeongcho extract, birch extract, gallja extract, active extract, honbon extract, rhubarb extract, ginko extract, baekjajam extract, Plant-derived herbal ingredients such as white extract, betel extract, leaflet extract, turmeric extract, peony extract, red peony extract, engraver extract, thyme extract, fennel extract, native extract, mold extract, cow extract, tea extract, bovine extract Can be used. These herbal medicines are generally available in the process of the Korean Pharmacopoeia or herbal medicine standard, and are generally available on the market. The extracts of these herbal medicines are obtained by a conventional solvent extraction method and are not particularly limited in selecting a solvent.

In the composition of the present invention, the content of the 5α-reductase activity inhibiting component is preferably 0.001 to 10.0% by weight, particularly preferably 0.01 to 5.0% by weight, based on the total composition.

In addition, the composition of the present invention, in order to enhance the action of ginseng extract or 5α-reductase activity inhibiting ingredients and to impart additional functions suitable for use by alopecia patients, supplements such as anti-dandruff, keratin softeners, refreshing agents, moisturizing agents, etc. The components can be added, in which case a better effect is obtained.

Examples of auxiliary components, such as anti-dandruff agents, keratin softeners, coolants, and moisturizers, which are incorporated into these conventional hair loss prevention agents and hair growth promoters, include hinokithiol, capsicum tink, nicotinic acid benzyl, pyroxtonolamine, salicylic acid, and el-menthol. have. These auxiliary ingredients do not limit the concentration used in the range in which the dosage form can be maintained without disturbing the effect of the main ingredient, but in consideration of human safety or formulation stability, 0.001 to 0.1% by weight of hinokithiol and 0.001 ～ to red pepper tink. 10% by weight, benzyl nicotinate is added in an amount of 0.001 to 0.1% by weight, pyrotonolamine in an amount of 0.001 to 1.0% by weight, salicylic acid and el-menthol in 0.01 to 1.0% by weight, respectively.

Hair growth promoter composition of the present invention can be prepared in any formulation that can be applied to the scalp, such as liquid, cream, paste, or solid phase, shampoo, hair conditioner, hair lotion for promoting hair growth by adding conventional additives It may be prepared in a composition such as a liquid wool type, and this also includes an aerosol type of these formulations.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these Examples are only illustrative of the present invention, and the scope of the present invention is not limited to these.

In the effect test examples below, the C57BL / 6 mouse model was used as an animal test model for evaluating hair growth induction promoting effect. The C57BL / 6 mouse has black hair and has spontaneous alopecia. In addition, melanocytes (melanocytes) are present only in the hair follicles, and melanin synthesis matches well with the hair growth cycle, which has the advantage of determining the hair growth cycle in terms of skin color. It is widely used for research.

Preparation Example: Preparation Method of Samsam Extract

Samsam extract used in the Examples of the present invention was prepared as follows:

First, the roots of the ginseng were chopped and passed through a sieve No. 10, and about 10 times the amount of ethanol of the Korean medicinal product was added thereto, followed by cooling at room temperature for 5 days. The extracted stock solution was filtered using Whatman # 2 filter paper, and the filtered extract was evaporated completely at 45 ° C. using a vacuum condenser to obtain a dried extract. The yield was about 2-4.5%.                     

Effect Test Example 1 Evaluation of Hair Growth Induction Promoting Effect of Samsam Extract

The hair growth induction promoting effect experiment was used 42-56 days old mice (C57BL / 6, male), which is widely used for the evaluation of hair growth induction action.

First, the hair on the back of the mouse was removed using an electric razor, and each mouse was weighed and divided into 12 pieces so that the weight was evenly distributed. After one day of adaptation, the control drug and ginseng extract were applied to the hair removal site from the next day. At this time, 40% ethanol was used as a reference drug, and the concentrated solid of Samsam extract prepared in Preparation Example was diluted with 40% ethanol and used at various concentrations. 100 µl each was applied to the back of the mouse once a day for 28 days to observe the growth-induction promoting effect. The results were obtained by using an image analyzer to determine the ratio of the area where the newborn hair was grown to the area from which hair was removed. Evaluated. Table 1 below shows the results of the hair growth phase induction promoting action of ginseng extract, the effect according to the concentration.

Samsam extract concentration 0.001% 0.01% 0.1% One% 10% Control group Area ratio (%) 18.8 42.6 59.2 80.4 88.2 11.0

As shown in Table 1, the four ginseng extract showed a very good hair growth phase induction promoting action in a concentration-dependent manner. At concentrations lower than 0.01%, hair growth phase induction was better than the control, but not satisfactory. At concentrations higher than 10%, the action was not sufficient.

Effect Test Example 2 Evaluation of Increased Hair Follicle Activity of Samsam Extract

Human scalp tissue after scalp surgery was obtained from plastic surgery and hair follicle tissue was separated therefrom. That is, the scalp tissue was sterilized by immersion in 70% ethanol for 1 minute and washed three times with M199, a cell culture medium. Under the stereo microscope, the epidermis and the dermis were cut out with a surgical scalpel to separate the epidermis, and hair follicles embedded in the dermis were pulled out one by one using tweezers. The isolated hair follicles were transferred to M199 culture solution containing 10% fetal bovine serum (FBS), and the lower part of the hair follicles containing the dermal papilla was cut with a scalpel. The teat was separated using fine tweezers and transferred to M199 culture medium containing 20% FBS and incubated for 2 weeks. When cells grew from the dermal papilla tissue and filled with the culture vessel, the cells were separated from the culture vessel with 0.25% trypsin and 0.02% EDTA, followed by subculture.

2 ~ 3 passages were diluted with M199 medium containing 10% FBS at a concentration of 2 × 10 ⁴ / mL, and 5 ml of each was placed in a 60 mm culture vessel and incubated in a 37 ° C. incubator for 24 hours. The next day, the culture medium containing FBS was removed, exchanged with the culture medium containing FBS, and the culture solution containing Samsam extract for 24 hours, followed by the addition of 10 μCi of radiation-labeled thymidine per plate. And further incubated for 24 hours. The cultured cells were washed three times with phosphate buffer and washed once with 5% trichloroacetic acid. Cells were dissolved in 0.1% sodium dodecyl sulfonate and 0.5 N sodium hydroxide solution, mixed in a scintillation cocktail, and the amount of radioisotope was measured using a beta-counter. Cell activity was evaluated by comparing how much uptake of radiolabeled thymidine into the cells.

As a result of measuring the hair follicle activity of ginseng extract through the thymidine uptake measurement method, it showed very good hair follicle cell activity at relatively low concentration (10 ^-4 ~ 10 ^-6 %). Table 2 shows the results of the hair follicle activity evaluation of the four ginseng extract, the effect according to the concentration.

Samsam extract concentration Control group 0.000001% 0.00001% 0.0001% Activity (%) 100 110 132 138

As can be seen from the above results, Samsam extract directly acts on hair follicle epithelium and is believed to exhibit hair growth promoting effect.

Examples 1-4 and Comparative Examples 1 and 2

According to the formulation of Table 3, in addition to the composition of Examples 1 and 2, including Samsam extract, and Samsam extract, Examples 3 and 4 further comprising a ginseng extract or clove extract known as a 5α-reductase activity inhibiting ingredient The compositions of Comparative Examples 1 and 2 were prepared, and the extract did not include Samsam extract.

Compounding ingredient / weight (%) Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 ethanol 40 40 40 40 40 40 Ginseng extract 0.5 1.0 0.5 0.5 - - Ginseng extract - - 0.5 - 0.5 - Clove Extract - - - 0.5 - 0.5 Polysorbate 60
(Tween 60) 0.5 0.5 0.5 0.5 0.5 0.5 Purified water Add until 100% by weight

Effect Test Example 3: Evaluation of hair growth promoting effect by animal experiment

With each of the compositions prepared in the composition ratio as shown in Table 3 above, the hair growth promoting effect experiment was carried out in the same manner as in the hair growth phase induction evaluation of the four ginseng extract. Extract used in the composition used a concentrated solid according to the preparation. Table 4 below shows the results of the hair growth phase induction promoting action evaluation.

division Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Area ratio (%) 52 45 67 78 92 88

As shown in Table 4 above, hair growth promoting action was also observed in Comparative Example 1 and Comparative Example 2 containing only the 5α-reductase activity inhibiting component, but was not satisfactory. Example 1 and Example 2 containing only the ginseng extract showed excellent hair growth promoting action, and furthermore, in Examples 3 and 4 simultaneously containing the ginseng extract or clove extract together with the ginseng extract, It showed more excellent hair growth promoting action than when used alone.

These results indicate that the synergistic effect on hair growth promoting effect is shown when the ginseng extract has 5α-reductase activity inhibitory action along with hair growth phase induction promoting action.

As can be seen from the above experimental results, in the embodiment containing both ginseng extract or clove extract together with the ginseng extract, hair growth phase induction promoting action and 5α-reductase activity inhibition, respectively, which are related to hair loss prevention or hair growth promoting action, respectively. Different mechanisms, such as action, showed very good hair growth promoting effect, and none of the ingredients showed the effect without affecting the action of other active ingredients. Therefore, when used in combination with ginseng extract and the component having the inhibitory action of 5α-reductase activity, it provides a variety of actions for the hair loss phenomenon intertwined by various complex mechanisms, rather than showing a single action by any one component It is expected that much better hair loss prevention or hair growth promoting effect can be expected.

Subsequently, according to the present invention, a composition comprising an auxiliary ingredient together with ginseng extract and an active ingredient having an inhibitory effect of 5α-reductase activity was prepared as a liquid wool agent, and clinical trials were conducted in persons with alopecia.

Example 5

A composition containing a ginseng extract and other auxiliary ingredients together with four ginseng extracts according to the present invention was prepared by the following prescription.

0.5% by weight of ginseng extract;

0.5% by weight of ginseng extract;

0.05% by weight of hinokithiol, 1.0% by weight of red pepper tin, 0.01% by weight of benzyl nicotinate, 0.1% by weight of pyrotonolamine, 0.1% by weight of salicylic acid, 0.1% by weight of L-menthol;

Ethanol 40.0 wt%, purified water 57.64 wt%; (100 wt% total)

Effect Test Example 3: Clinical Evaluation of a Composition Containing Ginseng Extract and Other Auxiliary Components with Samsam Extract

For the composition prepared in Example 5, in order to confirm the effect in human clinical trials were selected and tested for alopecia subjects.                     

Twenty-five drops of male and female adolescents and males with alopecia areata or with average daily hair loss of 100 or more per day were applied to the hair loss site 2-3 times a day (approximately 2 ml). It was carried out in a manner that was carried out, depending on the degree of effect confirmation was carried out from at least 1 month to a maximum of 4 months.

To determine the effect, the average daily hair loss at trituration, gross findings, and the degree of improvement of subjective symptoms were evaluated in five stages according to the following evaluation scale (Table 5), and overall overall improvement was taken as the average of the evaluation scale. The final verdict was that the overall improvement was 3.0 points or more, and the results are shown in Table 6 below.

Phase division Hair Loss at Tricycle Subjective symptoms Visual judgment 5
4
3
2
One A marked decrease
Moderate decrease
Hardness decrease
immutability
Worsening A marked improvement
Moderate improvement
Hardness improvement
immutability
worse A marked improvement
Moderate improvement
Hardness improvement
immutability
worse

division Hair Loss at Tricycle Subjective symptoms Visual judgment Total improvement Example 3.8 4.3 3.9 4.0

As shown in Table 6 above, the composition of Example 5 containing 5α-reductase activity inhibitory ingredient and other auxiliary ingredients in combination with ginseng extract according to the present invention, improved hardness in hair loss, subjective symptoms and visual determination, etc. It can be seen that the effect of more than moderate to moderate improvement is very excellent in preventing hair loss and promoting hair growth. In addition, no adverse effects were found in all subjects during the trial period, indicating that the composition is safe for humans.                     

Taken together, Samsam extract was found to have an excellent effect on hair follicle cells as well as promoting hair growth induction in an animal model. In addition, when the combination of the ginseng extract and the 5α-reductase activity inhibitory component was combined, it showed a very good effect in the experiments with animals and clinical trials for hair loss patients compared to the use alone. Therefore, when appropriately used in combination of these two components, by providing a variety of actions to the hair loss phenomenon entangled by various complex mechanisms, it is much better to prevent hair loss and promote hair growth than to exhibit a single action by any one component It can be seen that.

According to the present invention, a hair growth accelerator composition containing four ginseng extracts having excellent growth phase induction promoting action and hair follicle cell activation action as an active ingredient for promoting hair growth has little side effects and exhibits excellent hair growth promoting and hair loss prevention effects. In addition, the composition according to the present invention is very safe even when it contains simultaneously the ingredients having a inhibitory action of 5α-reductase activity in combination with four ginseng extract and has an excellent hair loss prevention and hair growth promoting effect.

Claims (7)

A cosmetic composition having a hair loss prevention and hair growth promoting effect comprising a ginseng (沙 蔘, Adenophora triphylla ) extract and 5α-reductase activity inhibiting ingredient as an active ingredient. The cosmetic composition according to claim 1, wherein the content of the ginseng extract is 0.01 to 10% by weight of the total weight of the composition as a solid content. The cosmetic composition according to claim 1 or 2, wherein the extraction solvent of the samsam extract is one kind or a mixture of two or more kinds of lower alcohols having 1 to 4 carbon atoms. delete The cosmetic composition according to claim 1, wherein the content of the 5α-reductase activity inhibiting component is 0.001 to 10% by weight of the total weight of the composition. delete A coating-type preparation which can apply the cosmetic composition which has the hair loss prevention and hair growth promoting effect of Claim 1 to a scalp.