KR100858224B1 - Cosmetic composition for the preventing loss of hair and promoting growth of hair comprising the extracts of canavalia gladiata - Google Patents

Abstract

A cosmetic composition comprising the extract of Canavalia gladiate is provided to inhibit activity of 5 alpha-reductase which promotes production of dihydrotestosterone as male hormone, and activate hair papilla cells, so that the composition is useful to prevent loss of hair and promote growth of hair. A cosmetic composition for preventing loss of hair and promoting growth of hair comprises the extract of Canavalia gladiate which is prepared by drying and pulverizing Canavalia gladiate, extracting the pulverized Canavalia gladiate in extraction solvent selected from purified water, ethanol, methanol, isopropanol, n-butanol, glycerol, propyleneglycol, 1,3-butylene glycol, ethylacetate, methylacetate, benzene, n-hexane, diethylether and dichloromethane for 3 days, and filtering, concentrating and freeze-drying the extracted Canavalia gladiate, and is formulated as transdermal topical formulation such as liquid, cream, lotion, paste or solid.

Description

Cosmetic composition for hair loss preventing and hair growth containing hairdo extracts {Cosmetic composition for the preventing loss of hair and promoting growth of hair comprising the extracts of Canavalia gladiata}

The present invention relates to a cosmetic composition for hair having a hair loss prevention and hair growth promoting effect containing the extract.

Hair growth depends on the action of the hair follicle tissue. The hair follicle tissue is histologically composed of epithelial cells, and has a structure in which epithelial tissue is embedded toward the dermis. Hair follicles consist of outer root sheath cells, inner root sheath cells, matrix cells, and dermal papilla cells, each of which is morphologically morphological. Not only do they have a specific form, they are known to have unique functions.

In addition, these cells have reaction specificities for various growth factors and hormones. The dermal papilla is characterized by being composed of fibroblast-like cells in the dermis of mesodermal origin, whereas other cells of the hair follicle tissue are of ectoderm origin. The dermal papilla is located at the lowest end of the hair follicle tissue and is enclosed by surrounding epithelial cells.

Hair growth is thought to play an important role in regulating the division of epithelial cells since the epithelial cells surrounding the dermal papilla divide and form a hair shaft. . In addition, the site where male hormone acts on hair follicles, which is essential in male alopecia, is also known as breast papilla, which is known to play an important role in hair growth / hair loss.

In male expression, testosterone is involved in skeletal muscle increase, male external genital development, scrotum growth, and spermatogenesis, and dihydro-testosterone is known to be involved in tissues such as acne, sebum increase, alopecia and prostatic hyperplasia. (J Invest. Dermatol. 1995; 209-14).

Since male-type hair loss among male hormone-dependent diseases causes serious mental pain for men in the modern society, which emphasizes beauty, researches are being actively conducted to identify and improve the mechanism of hair loss.

5alpha-reductase is present in male hormone-reactive tissues such as sebaceous glands, hair follicles, prostate, or epididymis, and is a testosterone, a male hormone that is biosynthesized in testes, and is a dihydro-testosterone (DHT). It is known that NADPH is used as a coenzyme in the conversion process.

Excessive secretion of dihydro-testosterone after puberty acts as a major agent in the production of sebum in sebocytes (sebocytes), causing acne and male hair loss. In particular, hair follicle cells are thought to cause hair loss because dehydro-testosterone binds to hormone receptors better than testosterone.

Accordingly, studies are being actively conducted to develop a substance that inhibits the activity and production of 5-alpha-reductase, which promotes the production of dihydro-testosterone, and to use it as a hair regrowth or hair loss prevention agent.

Recently, Merck, USA, has synthesized finasteride (proastercar), a drug that inhibits the action of 5 alpha-reductase to inhibit the production of dihydro-testosterone, and is commercially available as a hair regrowth for oral administration. It is a situation that there is a limit to use, such as other side effects caused by oral administration.

Against this background, there have been many attempts to promote hair growth and prevent hair loss by applying or taking a composition containing the extract of the herbal ingredient on the scalp. White porcelain extract was used in Korean Patent Registration No. 444102 and high ginseng extract was used in Korean Patent Registration No. 484236. In addition, Korean Patent Registration No. 520935 discloses that hair growth promoting effect is obtained by mixing ginseng extract and red ginseng extract in minoxidil.

On the other hand, Dodu (Chickpea, Canavalia gladiata, Canavalia ensiformis) is a dicotyledonous annual herb that is a dicotyledonous herbaceous herb and is called small bean or kalkong because bean pods resemble small bean knives. Cultivated in Doduins include hemaglutinine and canavanine, as well as vitamins A and C and urease, a hydrolytic enzyme. According to Chinese herbal medicine dictionary (中藥 大 辭典), herbal herb (本草綱目), etc., not only did they take medicinal beans for medicinal use, but also used pods for medicinal use as external preparations. The plant's stems, leaves, and roots have also been used for medicinal purposes. Since ancient times, it has been used as a high-quality dish and herbal medicine for the treatment of hiccups, vomiting, and abdominal pain caused by deprivation, and it is still known to be effective in purulent inflammatory diseases such as sinusitis, hemorrhoids and gum disease. Nothing is known about the back.

Korean Patent Registration No. 563329 refers to concentrates obtained by mixing Dodu extract and licorice extract for allergy and inflammation treatment.In Korean Patent Registration No. 772494, hydrogels cast from herbal extracts such as Dodu are effective for atopic dermatitis. It is reported. In addition, the Republic of Korea Patent Application No. 2002-48073 discloses the use of the composition extracted with the white porcelain, hulls, as an acne treatment, and Republic of Korea Patent Registration No. 350911 referred to a method for producing an antimicrobial active extract isolated from the dodu . However, there is only a mention of the known effect of reducing inflammation, and so far, there have been no reports on the effects of hair loss prevention and hair growth promotion.

Therefore, the present invention is to provide a cosmetic composition for hair containing the coating extract.

In order to achieve the above object, the present invention provides a cosmetic composition for hair having a hair loss prevention and hair growth promoting effect comprising the extract (Soybean, Canavalia gladiata ).

The cabbage includes at least one selected from the group consisting of fruits (beans), pods, stems, leaves, and roots of cabbage.

After extracting dried Doo powder, it is dried for 3 days and then cooled in about 10 times the amount of the extraction solvent, and then the extraction stock solution can be obtained by filtration, concentration, and lyophilization. There is no particular limitation. Extraction solvents include lower alcohols such as purified water, ethanol, methanol, isopropyl alcohol, n-butanol, polyhydric alcohols such as glycerol, propylene glycol, 1,3-butylene glycol, ethyl acetate, methyl acetate, benzene, n- Hydrocarbon solvents, such as hexane, diethyl ether, and dichloromethane, are mentioned, for example, One or two or more types of these solvents can be mixed and used for extraction.

Dodu extract-containing composition of the present invention may further comprise a hair growth promoting auxiliary component. Hair growth promoter adjuvant includes 5 alpha-reductase inhibitory component, hair follicle and hair follicle active ingredient, hair follicle blood flow enhancing component, bactericidal ingredient, anti-dandruff agent, keratin softener, coolant, humectant and the like. Examples of components for each action are as follows.

1) 5-alpha-reductase inhibitory component

Green tea extract, finasteride, white porcelain extract, red ginseng extract, linoleic acid, gamma-linolenic acid, jojoba oil, olive oil, sunflower oil, avocado oil, soybean oil added lecithin, cloves extract, eulein extract, malt extract, turmeric extract, betel nut extract Extracts, medicinal herb extracts, nutmeg extracts, chajeon extracts, Kangjin fragrance extracts, licorice extracts, golgol extracts, beef palmitos, apple extracts, etc.

2) Hair root and hair follicle active ingredient

Sugar Extract, Ginseng Extract, Red Ginseng Extract, Cinnabar Extract, Clove Extract, Euphoria Extract, Raw Citrate Extract, Sewage Extract, Yellow Extract, Pantothenyl Ethyl, Dipanthenol, Henna Extract, Iris Extract, Hino Kitiole, Nicotinic Acid Amide Etc

3) Follicle cell blood flow enhancing component

Minoxidil, ginkgo extract, red ginseng extract, pepper tink, tocopherol acetate, benzyl nicotinate, etc.

4) Sterilization and keratin soluble components

Salicylic acid, henna extract, iris extract, pyrotonolamine, etc.

5) Antidandruff

Ketoconazole, green tea extract, etc.

6) Softener

Elmenthol etc.

7) Moisturizer

Oils such as olive oil, jojoba oil, camellia oil, sunflower oil, macadamia oil and avocado oil

The cosmetic composition of the present invention can be prepared in any transdermal topical formulation that can be applied to the scalp, such as liquid, cream, lotion, paste or solid phase, shampoo, hair for promoting hair growth by adding conventional additives It may be prepared from compositions such as conditioners, hair lotions, hair tonics, liquid wool types, and the like, including aerosol types of these formulations.

The present invention provides a cosmetic composition for hair having a hair loss prevention and hair growth promoting effect containing the extract of Dodu, wherein the dodu extract is 5-alpha-reducing enzyme that promotes the production of dihydro-testosterone, an active male hormone that causes hair loss. Inhibits hair loss and promotes hair growth through dermal papilla cell activity, thereby preventing hair loss and promoting hair growth.

Hereinafter, the present invention will be described in more detail. However, these Examples are only illustrative of the present invention, and the scope of the present invention is not limited to these.

Example 1 Preparation of Tofu Extract I

About 10 g of distilled water (DW) was added to 150 g of powdered dry curd and cooled for 3 days, and then the extract was filtered and concentrated under reduced pressure to obtain about 10 g of curd extract I.

< Example  2> Dodu  Preparation of Extract II

The dried green powder was powdered and dried in 150g of 70% ethanol (about 10 times), and cooled for 3 days, and then the extract was filtered and concentrated under reduced pressure to obtain about 12g of green bean extract II.

Example 3 Preparation of Tofu Extract III

150 g of powdered dry curd was added to about 10 times ethylacetate (ethylacetate), and the mixture was cooled for 3 days, and then the extract was filtered and concentrated under reduced pressure to obtain about 1 g of curd extract III.

< Production Example  1 to 5> Dodu  Preparation of Compositions Containing Extracts

According to the formulation of Table 1 below, a composition containing the extract of Dodu extract I prepared in Example 1, such as salicylic acid, nicotinic acid amide, and nicotinic acid, which are known as general hair regrowth effect ingredients, was prepared and prepared as Preparation Examples 1 to 5, respectively. . The preparations described in Table 1 below may be prepared in shampoos, hair conditioners, hair lotions, hair tonic formulations according to conventional methods.

<Comparative Examples 1 to 5> Preparation of a composition containing no coating extract

In the same manner as in Examples 1 to 5, the composition containing no coating extract was prepared according to the prescription of Table 1, and Comparative Examples 1 to 5 were prepared.

< Production Example  6> Dodu  Extracts Minoxidil  Preparation of the containing composition

A composition containing Dodu extract I prepared in Example 1 in minoxidil, known as a general hair regrowth effect ingredient, was prepared in a total amount of 100 ml according to the prescription of Table 2 below.

<Comparative Examples 6 and 7> Preparation of a composition containing no coating extract

In the same manner as in Example 6, the composition containing no coating extract was prepared so as to be 100 ml in total according to the formulation of Table 2, and Comparative Examples 6 and 7, respectively.

Experimental Example 1 Experiment for Evaluation of Hair Growth Promoting

C57BL / 6 mouse model was used as an animal test model used to evaluate hair growth promotion. The C57BL / 6 mouse was characterized by black hair, spontaneous alopecia, and melanocytes. Exclusively present only in the hair follicle, melanin (melanin) synthesis is well matched to the hair growth cycle (hair growth cycle) has the advantage of determining the growth cycle of the hair in the skin color has been widely used in hair growth promotion evaluation.

The hair growth evaluation test was performed using 42-56 days old mice (C57BL / 6, male) to remove the hair at the back and add 100 microliters of the extract and the control group to the back of each subject once a day. Apply daily. At this time, 30% ethanol was used as a control drug, and the extracts prepared in Examples 1 to 3 were diluted with 30% ethanol as test drugs and used at various concentrations. The ratio of the area where the newborn hair was grown to the area from which hair was removed was taken with a digital camera (Canon, Japan) and compared using an image analyzer (Image analyzer / Image-Pro Plus, USA) (Table 3, Figures 1 and 2). ).

As can be seen in Table 3, it was confirmed that the extracts of the extract of Examples 1 to 3 according to the present invention all have a higher hair growth phase induction effect compared to the control group.

Experimental Example 2 Evaluation of Inhibitory Activity of 5-alpha-Reductase Activity of Dodous Extract

5-alpha-reductase was used in the liver (adult, Sprague-Dawley, male rats (8 ~ 10 week old, testis)) of the 5 alpha-reductase activity inhibition experiment. A protein suspension was prepared, and an enzyme reaction solution was prepared in a general manner.

Radiolabeled testosterone ( ³ H testosterone) was used as a substrate of 5 alpha-reductase, and the ratio of (enzyme reaction solution: protein suspension: dodo extract = 70:20:10) was 30. After reacting in an incubator for a minute, the mixture was extracted with ethyl acetate, 100 microliters of ethyl acetate phase was developed on a thin layer chromatography (TLC), and the conversion rate to the developed testosterone was measured. 5 alpha -reductase inhibition rate was evaluated by comparing with the control group (conversion rate without the dodo extract added). The inhibition rate calculation method is as follows.

Conversion rate = amount of radiation in the area of DHT / total amount of radiation

5 Alpha-reductase activity inhibition rate (%) = [(A-B) / A] X 100

A = conversion of testosterone to dihydro-testosterone without test substance

B = conversion of testosterone to dihydro-testosterone upon addition of test substance

Experimental results by the inhibition rate calculation method is shown in Table 4 below (Fig. 3).

As shown in Table 4, the extract of Example 1 according to the present invention was evaluated to significantly inhibit the activity of 5-alpha-reductase even at low concentrations.

<Experiment 3> Experimental evaluation of dermal papilla cells activation

Human scalp tissue after scalp surgery was obtained from plastic surgery and hair follicle tissue was separated therefrom. In other words, the scalp tissue was sterilized by immersion in 70% ethanol for 1 minute and washed three times with M199, a cell culture medium. Under the stereo microscope, the epidermis and the dermis were cut out with a surgical scalpel to separate the epidermis, and hair follicles embedded in the dermis were pulled out one by one using tweezers. The isolated hair follicles were transferred to M199 culture solution containing 10% fetal bovine serum (FBS) and the lower part of the hair follicles containing the dermal papilla was cut with a scalpel. Using fine tweezers, the teat was separated and transferred to M199 culture solution containing 20% FBS and incubated for 2 weeks. When cells grew from the dermal papilla tissue and the culture vessel was full, the cells were separated from the culture vessel with 0.25% trypsin and 0.02% EDTA, followed by subculture.

2 ~ 3 passages of dermal papilla cells were diluted to 2 × 10 ⁴ / ml concentration with M199 culture solution containing 10% FBS, and then 5ml each of them were put into a 60mm culture vessel and incubated in 37 ℃ incubator for 24 hours. The next day, remove the culture medium containing FBS, incubate for 24 hours by exchanging the culture medium containing FBS and the culture solution containing Dodo extract. The time was incubated further. The cultured cells were washed three times with phosphate buffer and washed once with 5% trichloroacetic acid. Cells were dissolved in 0.1% sodium dodecyl sulfonate and 0.5 N sodium hydroxide solution, mixed in a scintillation cocktail, and the amount of radioisotope was measured using a beta-counter. Cell activity was evaluated by comparing how much uptake of radiolabeled thymidine into the cell compared to the control group.

As a result of measuring the dermal papilla cell activity of the extract of Dodus through the thymidine uptake measurement method, it showed very good dermal papilla cell activity at a relatively low concentration. Table 5 shows the results of the evaluation of the dermal papilla cells activity of the extract of Dodo in Example 1 of the present invention.

As can be seen in Table 5, the extract of the extract of Example 1 according to the present invention is directly acted on the dermal papilla cells, it is judged to exhibit a hair growth promoting effect.

< Experimental Example  4> Dodu  Experiment for promoting hair growth of the composition containing the extract

Using the same method as in Experimental Example 1 to evaluate the hair growth promoting effect of the composition containing the extract (Fig. 4, 5 and 6).

As shown in Table 6 above, the composition of Preparation Examples 1 to 5 containing the extract of Dodu according to the present invention showed a hair growth promoting effect of about two times higher than that of the comparative example.

Experimental Example 5 Evaluation of Hair Growth Acceleration of a Composition Containing Tooth Extract and Minoxidil

Using the same method as in Experimental Example 1, the hair growth promoting effect of the composition containing the extract was evaluated (Fig. 7, Fig. 8).

As shown in Table 7 above, the composition containing the extract of Dodu according to the present invention showed a high hair growth promoting effect compared to the comparative example. It can be seen that the hair growth promoting effect is increased when the domino extract in minoxidil, known as a general hair growth effect ingredient.

1 is a graph showing the results of Dodu extract hair growth promotion evaluation of Experimental Example 1,

Figure 2 is a photograph showing the results of the Dodu extract hair growth promotion evaluation of Experimental Example 1,

Figure 3 is a graph showing the results of the evaluation of the inhibition activity of 5 alpha-reductase activity of Dodu extract of Experimental Example 2,

Figure 4 is a graph showing the results of the hair growth promotion evaluation of the composition containing the extract Dodu extract of Experimental Example 4,

5 is a photograph showing the results of the hair growth promotion evaluation of the Comparative Example composition containing no dodo extract of Experimental Example 4,

Figure 6 is a photograph showing the results of the hair growth promotion evaluation of the composition containing the extract Dodu extract of Experimental Example 4.

7 is a graph showing the results of the hair growth promotion evaluation of the composition containing the extract of Dodusu of Experimental Example 5,

Figure 8 is a photograph showing the results of the hair growth promotion evaluation of the composition containing the extract Dodu extract of Experimental Example 5.

Claims (11)

A cosmetic composition for hair having a hair loss prevention and hair growth promoting effect, which contains the extract of Candua gladiata . According to claim 1, Hair growth prevention and hair growth containing a coating composition comprising a hair growth promoting auxiliary component, characterized in that the hair cosmetic composition having a hair growth promoting effect. The method of claim 2, wherein the hair growth promoting auxiliary component is selected from the group consisting of 5 alpha-reductase inhibitory component, hair follicle and hair follicle active ingredient, hair follicle blood flow enhancing component, bactericidal and keratin soluble component, anti-dandruff agent, refreshing agent, moisturizing agent Hair loss prevention and hair growth-containing cosmetic composition containing a coating of Dodous extract, characterized in that one or two or more. The method of claim 3, wherein the 5 alpha-reductase inhibitory components are green tea extract, finasteride, white porcelain extract, gosam extract, linoleic acid, gamma linolenic acid, jojoba oil, olive oil, sunflower oil, avocado oil, soybean oil-added lecithin, Cultivated extract, Euiin extract, gallnut extract, turmeric extract, betel nut extract, subtractive extract, cow nut extract, tea extract, kangjin extract, licorice extract, golgol extract, sopalmito, apple extract A cosmetic composition for hair having an hair loss prevention and hair growth promoting effect containing the extract. The active ingredient according to claim 3, wherein the hair root and hair follicle cell active ingredients are medicinal extracts, ginseng extract, red ginseng extract, thigh extract, cloves extract, oyster extract, raw yellow sulfur extract, sewage extract, yellow extract, pantothenyl ether, dipantenol, henna A hair cosmetic composition having an anti-hair loss and hair growth promoting effect, comprising a doubar extract, characterized in that the extract is selected from iris extract, hinokithiol, nicotinic acid amide and elmenthol. According to claim 3, The hair follicle blood flow enhancing component is a hair cosmetic containing hairdo extract and hair growth promoting effect, characterized in that selected from minoxidil, ginkgo extract, red ginseng extract, pepper tink, tocopherol acetate, nicotinic acid benzyl Composition. The cosmetic composition for hair having a hair loss preventing and hair growth promoting effect according to claim 3, wherein the bactericidal and keratin soluble components are selected from salicylic acid, henna extract, iris extract, and pyrotonolamine. 4. The hair cosmetic composition of claim 3, wherein the anti-dandruff agent is selected from ketoconazole and green tea extract. 4. The hair cosmetic composition according to claim 3, wherein the refreshing agent is elmenthol. The hair cosmetic composition of claim 3, wherein the moisturizing agent is selected from olive oil, jojoba oil, camellia oil, sunflower oil, macadamia oil, and avocado oil. A liquid, cream, lotion, paste or solid transdermal topical formulation containing the composition of claim 1.

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