KR20060095062A - Hair growth agent composition - Google Patents

Abstract

The present invention relates to a hair loss prevention and hair growth promoter composition containing an extract of Ulmus radics cortex, and as a active ingredient hair shortening the transition period from the resting phase to the growth phase of the hair growth cycle as a new hair The composition of the present invention, which contains a milk extract, which has excellent hair follicle cell activity and hair follicle cell death suppression, has excellent side effects, has excellent hair growth promoting and hair loss prevention effects, and inhibits 5α-reductase activity. Even when it contains the active ingredients at the same time is very safe and has excellent hair loss prevention and hair growth promoting effect.

Milky skin extract, 5 alpha-reductase, promote hair growth, hair follicle cell activity, inhibit hair follicle cell death, prevent hair loss.

Description

Hair growth promoter composition {HAIR GROWTH AGENT COMPOSITION}

The present invention relates to a hair loss prevention and hair growth promoter composition containing an extract of Ulmus radics cortex, more specifically, the present invention is to reduce the cycle of transition from the resting phase to the growth phase of the hair growth cycle as an active ingredient, The present invention relates to a hair growth promoter containing a milk extract extract having an excellent hair follicle cell activation effect along with a hair growth phase induction promoting effect that makes hair grow faster.

Human hair is about 100,000 to 150,000 pieces, each hair has a different cycle and grow and drop out through the anagen, catagen, telogen. This cycle is repeated over three to six years, resulting in an average of 50 to 100 hairs falling out per day. Generally, the term alopecia refers to the increase in the number of hairs that fall abnormally due to shortening of the hair growth rate in the growth phase and more hair in the degenerative or resting phase.

The causes of alopecia have been discussed, such as excessive male hormone action, excessive sebum secretion, poor blood circulation, hypoglycemia caused by peroxide or bacteria, genetic factors, aging, stress. However, to date, no clear cause for hair loss is unknown, and it is known that a very complicated action is intertwined.

As for the various causes of hair loss, hair loss prevention products known to date are effective ingredients for promoting blood circulation, strengthening hair root function, moisturizing scalp, preventing dandruff, antioxidant effect, hair growth period, and suppressing male hormone activity. It contains ingredients, etc., but there is still no clear effect in terms of effects and side effects may be raised.

Experimental methods used to prevent hair loss and promote hair growth are mainly used in vivo evaluation using experimental animals and in vitro evaluation using hair follicle cells and tissue culture. In the 1980s, research on the differentiation mechanism of hair follicles became active with the development of culture methods of dermal papilla cells and outer root sheath cells, which are the core cells of hair follicles. With the development of tissue culture methods, it has been shown to be a useful evaluation model for research on hair growth, wool and hair loss prevention. In recent years, biochemical and molecular biological experiments have been actively conducted to identify various growth factors and genes related to hair growth, research on mechanism of action, and drug search.

The effects of a commercially available product on the hair as a hair growth or hair loss preventing agent on the hair include growth phase induction effect, hair growth phase extension effect, 5α-reductase inhibitory effect, blood circulation promoting effect, bactericidal effect, anti dandruff effect, moisturizing effect, antioxidant effect, etc. However, in the existing formulations, hair loss prevention and hair growth promoting effects are not sufficient.

Androgenetic alopecia is directly dependent on the amount of androgen, which is dependent on androgen hormones. Accordingly, many studies have recently been reported to prevent and treat hair loss by inhibiting androgen activity. Briefly explain the mechanism of hair loss on male hormones are as follows. In other words, testosterone, one of the male hormones, is converted to dihydrotestosterone, an active male hormone, by an enzyme called 5α-reductase. Induces hair loss. In addition, such mechanisms may cause excessive production of sebum, causing acne, seborrheic dermatitis, and the like, and as a result, scalp may occur with inflammation. As a result, androgenetic alopecia is due to the excessive production of dihydrotestosterone by the action of 5α-reductase, it will be able to prevent and treat masculine alopecia by inhibiting the activity of 5α-reductase. In view of this, male hair loss treatment using finasteride, which has been used as a prostate treatment for men, has been developed, but side effects such as sexual dysfunction have been reported, and their 5α-reductase activity against hair loss It is also difficult to fully anticipate the effect by inhibitory effect alone.

Recently, the cause of hair loss is not limited to male hormones, and research on various hair loss mechanisms has been actively conducted. Among these, the newly emerging hair loss mechanism is as follows. In other words, hair loss is caused by the same process that causes hair to no longer grow as the hair cycle becomes shorter, regardless of the cause. More specifically, hair loss is a period from the growth stage to the degenerative stage compared to normal hair. This happens as it gets shorter. Based on these observations, the possibility of one of the factors leading to the retrograde phase as one of the causes of hair loss was examined, and research results showing that such factors exist in fact. These factors promote the death of the cells that make up the hair follicles, which in turn leads to the degenerative phase. A representative factor of the degenerative promoters known to date is Transforming Growth Factor (TGF) -beta 1.

In view of the above problems, the present inventors screened dozens of plant extracts through animal experiments that can confirm the growth-induction promoting action of hair among the effects on various mechanisms, the hair growth-induction induction effect from the milk extract It was found to be excellent, and also showed a very good activity in the experiment evaluating the hair follicle cell activity of the milk extract. In addition, in the evaluation of inhibition of apoptosis, it was proved that milk milk has an effect of inhibiting the action of transforming promoter-beta 1 (TGF-beta 1), which is an apoptosis promoting factor, and substantially inhibiting apoptosis, leading to the completion of the present invention.

Specifically, the present inventors conducted a study on hair growth induction promoting effect by selecting dozens of plant extracts having the effects of preventing hair loss and promoting hair growth for alopecia and securing safety to the human body. It was found that this new hair promotes the transition from the resting phase to the growth phase in the hair growth cycle where it grows and falls out again. In addition, the milk extract showed very good results in vitro evaluation of the activity of hair follicle cells.

On the other hand, Korean Patent No. 0163118 is a cosmetic detergent composition containing the milky skin extract, which contains a plant extract obtained by extracting milky skin with water, alcohol and acetone in an amount of 0.001 to 10% (w / w). The composition has a bactericidal, antibacterial and astringent action, and it is disclosed that it is effective in increasing acne healing and skin elasticity when used in a cosmetic soap, and reducing the scalp itching or dandruff and increasing hair shine when added to the shampoo. However, this cosmetic composition only describes the antibacterial and astringent effects of washing, and does not mention the functions related to promoting hair growth of the milky skin and preventing hair loss.

It is an object of the present invention to provide a hair growth promoter composition which has little side effects and has excellent hair growth promoting and anti-hair loss effects.

In order to achieve the above object, the present invention provides a hair loss prevention and hair growth promoter composition comprising an extract of Ulmus radics cortex as an active ingredient.

Here, the content of the milky skin extract is preferably 0.01 to 10% by weight of the total weight of the composition as a solid, and the extraction solvent of the milky skin extract may be one or two or more mixed solvents of a lower alcohol and a hydrocarbon solvent. .

In addition, the composition of the present invention may further comprise at least one 5α-reductase activity inhibiting component, wherein the content of the 5α-reductase activity inhibiting component is preferably 0.001 to 10% by weight.

In addition, the composition of the present invention may be included in a transdermal topical formulation that can be applied to the scalp, such as a liquid, cream, paste, aerosol.

According to the present invention, the composition including the milky skin extract, the milky skin extract not only has a clear mechanism of action of promoting hair growth phase, but also has an active action on hair follicle cells, thereby exhibiting hair growth promoting effect. In addition, when the extract contains 5α-reductase activity inhibitory ingredient as an active ingredient together with the milky skin extract, hair loss prevention and hair growth promoting effect may be more excellent.

Hereinafter, the composition of this invention is demonstrated more concretely.

First, in view of the background of the present invention, in order to prevent hair loss and promote hair growth for alopecia, various hair loss mechanisms or mechanisms of action should be approached through a variety of mechanisms. The study was examined. First of all, we have screened hundreds of combinations of male hormone-regulating components, blood circulation promoting components, cell activating components, and anti-inflammatory components, and hair loss prevention and hair growth promoting effects. As a result, the milk extract was found to have excellent hair growth induction promoting action and hair follicle cell activity. In addition, it was evaluated whether there is an effect on inhibiting apoptosis, a new hair loss mechanism, and as a result, the apoptosis was significantly reduced by the milk extract, and the effect of TGF-beta 1 was significantly reduced. It was. Furthermore, it was found that the composition including the 5α-reductase activity inhibitory ingredient for controlling male hormone action together with the milk extract is not only excellent in preventing hair loss and promoting hair growth, but also exhibits no side effects. Therefore, in the present invention, from the viewpoint of hair growth stage induction promoting action, hair follicle activating action, hair follicle cell death inhibiting action, 5α-reductase activity inhibiting action and the like, which are evaluated as important factors for hair growth and hair loss prevention, It provides a composition having a hair loss prevention, hair growth promoting action through a complex action for.

Milky blooming in the composition of this invention (Ulmus radics cortex), the king of ulmaceae plants respectively distributed in the central Korea north of China and Japan, elm (Ulmus macrocarpa), Ulmus pumila (Ulmus pumila), elm (Ulmus davidiana) The root portion of the stem or root portion of the root is taken and dried in the sun. Its main active ingredient is known to contain beta-sitosterol (β-Sitosterol), plant sterols and tannins.

The extract of the milky skin used in the hair cosmetic composition of the present invention is prepared as follows: First, the dried milky skin herb is dried and powdered, and then, about 10 times the amount of the extraction solvent is added to the milky skin powder, and about 3 After immersion extraction daily, the extract is filtered, concentrated and freeze-dried to obtain a dry powder of milky skin extract.

At this time, the extraction solvent is not particularly limited in the selection, purified water; Lower alcohols such as methanol, ethanol, isopropyl alcohol and n-butanol; Polyhydric alcohols such as glycerol, propylene glycol and 1,3-butylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane, and the like. Among these solvents, it is preferable to use one kind or a mixture of two or more kinds of lower alcohols such as methanol, ethanol and n-butanol.

The milky skin extract thus prepared is preferably included as a solid content in 0.01 to 10% by weight of the total weight of the composition of the present invention. If it is less than 0.01% by weight, the hair growth phase induction promoting effect is not satisfactory, and at a concentration higher than 10% by weight, the effect thereof is not sufficient compared to the dose, which is not preferable.

The 5α-reductase activity inhibiting component which may be included in the composition of the present invention together with the milky skin extract is a compounding ingredient of an external preparation that exhibits a hair loss prevention effect by inhibiting the action of 5α-reductase in human hair follicles. It does not specifically limit unless it is, and it can mix | blend individually or in combination of 2 or more components. Such 5α-reductase activity inhibitory ingredients include, for example, ginseng extract, uiyiin extract, clove extract, Bupyeongcho extract, birch extract, gallja extract, active extract, honbon extract, rhubarb extract, ginko extract, baekjajam extract, Plant-derived herbal ingredients such as white extract, betel extract, leaflet extract, turmeric extract, peony extract, red peony extract, engraver extract, thyme extract, fennel extract, native extract, mold extract, cow extract, tea extract, bovine extract Can be used. These herbal medicines are generally available in the process of the Korean Pharmacopoeia or herbal medicine standard, and are generally available on the market. The extracts of these herbal medicines are obtained by a conventional solvent extraction method and are not particularly limited in selecting a solvent.

In the composition of the present invention, the content of the 5α-reductase activity inhibiting component is preferably 0.001 to 10.0% by weight, particularly preferably 0.01 to 5.0% by weight, based on the total composition.

In addition, the composition of the present invention, such as anti-dandruff agents, keratin softeners, refreshing agents, moisturizers, etc. in order to enhance the action of the milk extract extract or 5α-reductase activity inhibiting ingredients and to impart additional functions suitable for use by alopecia patients. Auxiliary components can be added, in which case a better effect is obtained.

Examples of auxiliary components, such as anti-dandruff agents, keratin softeners, coolants, and moisturizers, which are incorporated into these conventional hair loss prevention agents and hair growth promoters, include hinokithiol, capsicum tink, nicotinic acid benzyl, pyroxtonolamine, salicylic acid, and el-menthol. have. These auxiliary ingredients do not limit the concentration used in the range in which the dosage form can be maintained without disturbing the effect of the main ingredient, but in consideration of human safety or formulation stability, 0.001 to 0.1% by weight of hinochithiol and 0.001 to 0.1% of red pepper tink. 10% by weight, benzyl nicotinate is added in an amount of 0.001 to 0.1% by weight, pyrotonolamine in an amount of 0.001 to 1.0% by weight, salicylic acid and el-menthol in 0.01 to 1.0% by weight, respectively.

Hair growth promoter composition of the present invention can be prepared in any formulation that can be applied to the scalp, such as liquid, cream, paste, or solid phase, shampoo, hair conditioner, hair lotion for promoting hair growth by adding conventional additives It may be prepared in a composition such as a liquid wool type, and this also includes an aerosol type of these formulations.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these Examples are only illustrative of the present invention, and the scope of the present invention is not limited to these.

In the effect test examples below, the C57BL / 6 mouse model was used as an animal test model for evaluating hair growth induction promoting effect. The C57BL / 6 mouse has black hair and has spontaneous alopecia. In addition, melanocytes (melanocytes) are present only in the hair follicles, and melanin synthesis matches well with the hair growth cycle, which has the advantage of determining the hair growth cycle in terms of skin color. It is widely used for research.

Preparation Example: Method of Preparing Milky Skin Extract

The milky skin extract used in the Examples of the present invention was prepared as follows: First, the root of the milky skin was chopped and passed through No. 10 sieve, and then about 500 ml of the medicinal product ethanol was added to 50 g of powder, and the mixture was dried at room temperature for 3 days. Chilled. The extracted stock solution was filtered using Whatman # 1 filter paper, and the filtered extract was evaporated completely at 45 ° C. using a vacuum concentrator to obtain a dried extract. Yield was about 5%.

Effect Test Example 1 Evaluation of Hair Growth Induction Promoting Effect of Milky Skin Extract

The hair growth phase induction promoting effect experiment was used 42-56 days old mice (C57BL / 6, male), which is widely used for the evaluation of hair growth phase induction action.

First, the hair on the back of the mouse was removed using an electric razor, and each mouse was weighed and divided into 12 pieces so that the weight was evenly distributed. After the adaptation period for one day, the control drug and the milky skin extract were applied to the hair removal site from the next day. At this time, 40% ethanol was used as a reference drug, and the concentrated solid of the milk extract extracted in Preparation Example was used by diluting it at various concentrations with 40% ethanol. 100 µl each was applied to the back of the mouse once a day for 28 days to observe the growth-induction promoting effect. The results were obtained by using an image analyzer to determine the ratio of the area where the newborn hair was grown to the area from which hair was removed. Evaluated. Table 1 below shows the results of the hair growth phase induction promoting action of the milk extract, showing the effect of concentration.

Milky Skin Extract Concentration 0.001% 0.01% 0.1% One% 10% Control Area ratio (%) 21.0 33.9 52.6 68.7 79.8 13.0

As shown in Table 1, the milky skin extract showed a very good hair growth induction promoting action in a concentration-dependent manner. At concentrations lower than 0.01%, hair growth phase induction was better than the control, but not satisfactory. At concentrations higher than 10%, the action was not sufficient.

Effect Test Example 2: Evaluation of the increase of hair follicle activity of the milk extract

Human scalp tissue after scalp surgery was obtained from plastic surgery and hair follicle tissue was separated therefrom. First, the scalp tissue was sterilized by immersion in 70% ethanol for 1 minute and washed three times with DMEM, a cell culture medium. Under the stereo microscope, the epidermis and the dermis were cut out with a surgical scalpel to separate the epidermis, and the hair follicles embedded in the dermis were pulled out one by one using tweezers. The isolated hair follicles were transferred to DMEM medium containing 10% fetal bovine serum (FBS), and the lower part of the hair follicles containing the dermal papilla was cut with a scalpel. The teat was separated using fine tweezers and transferred to a DMEM medium containing 20% FBS and incubated for 2 weeks. When cells grew from the dermal papilla tissue and filled with the culture vessel, the cells were separated from the culture vessel with 0.25% trypsin and 0.02% EDTA, followed by subculture.

2 ~ 3 passages of dermal papilla cells were diluted with DMEM medium containing 10% FBS to a concentration of 2 × 10 ⁴ / mL, and then 5mL each of them were put into a 60 mm culture vessel and incubated in a 37 ° C incubator for 24 hours. The next day, the culture medium containing FBS was removed, exchanged with the culture medium containing FBS and the milk extract, and cultured for 24 hours, and then the radiation-labeled thymidine was added 10 μCi per plate. Added and incubated for 24 hours. The cultured cells were washed three times with phosphate buffer and washed once with 5% trichloroacetic acid. Cells were dissolved in 0.1% sodium dodecyl sulfate and 0.5 N sodium hydroxide solution, mixed in a scintillation cocktail, and the amount of radioisotope was measured using a beta-counter. Cell activity was evaluated by comparing how much uptake of radiolabeled thymidine into the cells.

As a result of measuring the hair follicle activity of the milky skin extract through the thymidine uptake measurement method, the hair follicle cell activity was shown to be excellent at relatively low concentration (10 ^-4 to 10 ^-6 %). . The following Table 2 shows the hair follicle activity evaluation results of the milky skin extract, showing the effect of concentration.

Milky Skin Extract Concentration Control 0.000001% 0.00001% 0.0001% Activity (%) 100 113 129 138

As shown in the above results, it is judged that the milk extract extracts directly act on the hair follicle epithelium and exhibit hair growth promoting effect.

Experimental Example 3 Evaluation Evaluation of Hair Follicle Cell Inhibition of Milky Skin Extract

After culturing the cells in the same manner as the method used for cell activity evaluation, diluting 2-3 papillary dermal papilla cells to 2 × 10 ⁴ / mL concentration with DMEM medium containing 10% FBS, and then in a 60 mm culture vessel 5 ml each was incubated in a 37 ℃ incubator. After incubation for 12 hours, the culture medium containing the FBS was removed, and the culture medium containing the FBS and the culture solution containing the milk extract were incubated for 24 hours. The following day, 300 μM of hydrogen peroxide was treated to induce cell death, and after 24 hours, cells were detached using 0.25% trypsin, 0.02% EDTA, and collected in a tube. 3 ml of phosphate buffer was added to each tube and washed three times using a centrifuge. Cells were fixed using 70% cold ethanol and phosphate buffer at a ratio of 9: 1, and washed three times with phosphate-citric acid buffer using a centrifuge. After adding RNA degrading enzyme A (RNase A) and reacting at 37 ° C. for 30 minutes, the cells were stained using a fluorescent dye, and the distribution of the killed cells was measured by measuring the cell cycle using a flow cytometer.

Measurement result, when the hydrogen peroxide treatment, compared to the 84% of the cells that caused apoptosis, ^10-4 to 10 when the handle after the pre-treatment blood milky extract of ^6% hydrogen peroxide, the degree of cell death caused a 12-24 As much as%, it was confirmed that apoptosis was significantly reduced. In particular, the apoptosis rate when treated with 10 ^-4 % milky skin extract was 12%, similar to that of normal cells (10%), indicating that the cell death rate was restored to normal by the milky skin extract. Can be. Table 3 shows the results of hair follicle activity evaluation of the milk extract.

Treatment Material Control Hydrogen peroxide Milky Skin Extract Treatment concentration - 300 μM 0.000001% 0.00001% 0.0001% Apoptosis (%) 10 84 24 19 12

Effect Test Example 4 Evaluation Evaluation of Inhibition of Transformation Accelerator Beta 1 of Milky Skin Extract

2 ~ 3 passages of papilla cells cultured in the same manner as the cell culture method was diluted to 2 × 10 ⁴ / ㎖ concentration with DMEM medium containing 10% FBS, and then 5 ml each of the 60 mm culture vessel incubated overnight It was. The next day, the culture medium containing FBS was removed and exchanged with a culture medium containing no FBS, a culture medium containing TGF-beta 1, or a culture medium containing both TGF-beta 1 and baekbaekpi extracts. Radiation-labeled thymidine was added 10 μCi per culture dish and incubated for 24 hours more. The cultured cells were washed three times with phosphate buffer and washed once with 5% trichloroacetic acid. Cells were dissolved in 0.1% sodium dodecyl sulfate and 0.5 N sodium hydroxide solution, mixed in a scintillation cocktail, and the amount of radioisotope was measured using a beta-counter. Cell activity was evaluated by comparing how much uptake of radiolabeled thymidine into the cells. Table 4 shows the results of the inhibitory evaluation of the transformation promoter beta 1 of the milk extract.

TGF-beta 1 Concentration Control 10 ng / ml 10 ng / ml 10 ng / ml 10 ng / ml Milky Skin Extract Concentration - - 0.000001% 0.00001% 0.0001% Activity *%) 100 58 94 103 117

As shown in Table 4 above, when TGF-beta 1 alone was treated, cell growth was reduced, but when TGF-beta 1 and milk extract were simultaneously treated, cell growth was more active than the control.

Examples 1-4 and Comparative Examples 1 and 2

According to the formulation of Table 5, in addition to the composition of Examples 1 and 2 containing the milky skin extract, and Example 3 further comprising a ginseng extract or clove extract known as 5α-reductase activity inhibiting component And 4, and the compositions of Comparative Examples 1 and 2, which did not include the milky skin extract.

Compounding ingredient / weight (%) Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 ethanol 40 40 40 40 40 40 Milky Skin Extract - - 0.5 1.0 0.5 0.5 Ginseng extract 0.5 - - - 0.5 - Clove Extract - 0.5 - - - 0.5 Polysorbate 60 (Tween 60) 0.5 0.5 0.5 0.5 0.5 0.5 Purified water Add until 100% by weight

Effect Test Example 5: Evaluation of Hair Growth Promotion Effect by Animal Experiment

With each composition prepared in the composition ratio as shown in Table 5 above, the hair growth promoting effect experiment was carried out in the same manner as in the evaluation of the hair growth phase induction effect of the milky skin extract. Extract used in the composition used a concentrated solid according to the preparation. Table 6 below shows the results of the hair growth phase induction promoting action evaluation.

division Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Area ratio (%) 47 41 67 77 81 85

As shown in Table 6, Comparative Example 1 and Comparative Example 2 containing only the 5α-reductase activity inhibitory component showed hair growth promoting action, but was not satisfactory. Example 1 and Example 2 containing the milk extract alone showed excellent hair growth promoting action, and furthermore, in Examples 3 and 4 simultaneously containing the red ginseng extract or the clove extract together with the milk extract, It showed a better hair growth promoting action than when the component alone.

These results indicate that the synergistic effect on the hair growth promoting action when the 5α-reductase activity inhibitory action along with the hair growth phase induction promoting action of the milky skin extract.

As can be seen from the above experimental results, in Examples containing both ginseng extract and cloves extract together with the milk extract, the hair growth phase induction promoting action, 5α-reductase activity associated with hair loss prevention or hair growth promoting action, respectively Different mechanisms, such as inhibitory action, showed very good hair growth promoting effect, and none of the ingredients showed the effect without affecting the action of other active ingredients. Therefore, when used in combination with an ingredient having an inhibitory effect of 5α-reductase activity in combination with the milky skin extract, it provides a variety of actions against hair loss entangled by various complex mechanisms, thereby exhibiting a single action by one component. It is expected that much better hair loss prevention or hair growth promoting effect can be expected.

Subsequently, according to the present invention, a composition comprising an auxiliary ingredient together with the milky skin extract and the active ingredient having an inhibitory effect of 5α-reductase activity was prepared as a liquid wool agent, and clinical trials were conducted in persons with alopecia.

Example 5

A composition containing a ginseng extract and other auxiliary ingredients together with the baekbaekpi extract according to the present invention was prepared by the following prescription.

Milky skin extract 0.5% by weight;

0.5% by weight of ginseng extract;

0.05% by weight of hinokithiol, 1.0% by weight of red pepper tin, 0.01% by weight of benzyl nicotinate, 0.1% by weight of pyrotonolamine, 0.1% by weight of salicylic acid, 0.1% by weight of L-menthol;

Ethanol 40.0 wt%, purified water 57.64 wt%; (100 wt% total)

Experimental Example 6 Clinical Evaluation of a Composition Containing Ginseng Extract and Other Auxiliary Components with Milk Extract

For the composition prepared in Example 5, in order to confirm the effect in human clinical trials were selected and tested for alopecia subjects.

Thirty-five drops (approximately 2 ml) were applied to the hair loss site for 15 males and females with male or female alopecia or daily average hair loss of 100 or more. It was carried out in a manner that was carried out, depending on the degree of effect confirmation was carried out from at least 1 month to a maximum of 4 months.

To determine the effect, the average daily hair loss at trituration, gross findings and degree of improvement of subjective symptoms were evaluated in five stages according to the following evaluation scale (Table 7), and overall improvement was taken as the average of the evaluation scale. The final verdict was that the overall overall improvement was more than 3.0, and the results are shown in Table 8 below.

Phase division Hair Loss at Tricycle Subjective symptoms Visual judgment 5 4 3 2 1 Significant decrease moderate decrease hardness hardness worse (increase) Significant Improvement Moderate Improvements Hardness Improvements Constant Deterioration Significant Improvement Moderate Improvements Hardness Improvements Constant Deterioration

division Hair Loss at Tricycle Subjective symptoms Visual judgment Overall improvement Example 3.7 4.0 3.8 3.8

As shown in Table 8 above, the composition of Example 5 containing the 5α-reductase activity inhibitory component and other auxiliary components together with the milky skin extract according to the present invention, the amount of hardness in hair loss, subjective symptoms and visual determination, etc. It can be seen that the effect of improving to moderate improvement is very excellent in preventing hair loss and promoting hair growth. In addition, no adverse effects were found in all subjects during the trial period, indicating that the composition is safe for humans.

Taken together, the results showed that the milk extract was excellent in the hair growth phase induction promoting effect as well as in the hair follicle cells in the animal experiment model, and in addition, significantly reduced hair follicle cell death and promoted the degenerative phase. It also inhibited the action of TGF-beta 1. In addition, the combination of 5α-reductase activity inhibitory ingredient with the milk extract showed a very good effect in experiments using animals and clinical trials in alopecia patients compared to when used alone. Therefore, when appropriately used in combination of these two components, by providing a variety of actions to the hair loss phenomenon entangled by various complex mechanisms, it is much better to prevent hair loss and promote hair growth than to exhibit a single action by any one component It can be seen that.

According to the present invention, the hair growth promoter composition containing the milky skin extract excellent in hair growth phase induction promoting action, hair follicle cell activation action, and hair follicle cell death suppressing effect as an active ingredient for promoting hair growth has little side effects and promotes excellent hair growth. And hair loss prevention effect. In addition, the composition according to the present invention is very safe even when it contains a component having an inhibitory action of 5α-reductase activity together with the milky skin extract and has an excellent hair loss prevention and hair growth promoting effect.

Claims (7)

Hair loss prevention and hair growth promoter composition comprising an extract of Ulmus radics cortex as an active ingredient. According to claim 1, wherein the content of the extract of the milky skin is a composition, characterized in that 0.01 to 10% by weight of the total weight of the composition as a solid. The composition of claim 1 or 2, wherein the extraction solvent of the milky skin extract is one or two or more mixed solvents of a lower alcohol and a hydrocarbon solvent. The composition of claim 1, further comprising a 5α-reductase activity inhibiting component. The composition according to claim 4, wherein the content of the 5α-reductase activity inhibiting component is 0.001 to 10% by weight. The method according to claim 4 or 5, wherein the 5α-reductase activity inhibitory ingredients are red ginseng extract, Euiin extract, cloves extract, Bupyeongcho extract, birch extract, gall bladder extract, active extract, botanical extract, rhubarb extract, cabbage extract 1 of zinnia extract, white powder extract, betel nut extract, leaflet extract, turmeric extract, peony extract, red peony extract, sculptural extract, thyme extract, fennel extract, garden extract, mold dog extract, cow extract, tea extract and bovine extract A composition characterized in that the species or more. A transdermal topical formulation for application to the scalp, comprising the hair loss prevention and hair growth promoter composition of claim 1.