KR20110046624A - Composition formed by using mixture of herbal extracts and Muskrat Ondatra zibethicusmusk for Anti-aging - Google Patents

Abstract

PURPOSE: A complex herb medicinal composition containing Muskrat (Ondatra zibethicus)musk extract and green tea is provides to ensure free radical removal, NF-kappa B suppression, moisturizing, and collagen fiber biosynthesis. CONSTITUTION: A complex herb medicinal composition for preventing or treating aging contains Muskrat (Ondatra zibethicus)musk extract, Rhei Rhizoma, Angelicae dahuricae Radix, Angelicae gigantis Radix, Snakegourd Root, Lonicerae Flos, Santalini Lignum rubrum, Xanthii Fructus, Coicis Semen, Cervi Cornu, and green tea. The composition further contains alcohol, oil, surfactant, fatty acid, silicon, wetting agent, moisturizing agent, emulsion, stabilizing agent, UV protector, coloring agent, or perfume. The composition is manufactured in the form of lotion, essence, cream, pack, gel, ointment, patch, or spray.

Description

Anti-aging composite herbal composition containing musk extract and herbal extract from musk rat {Composition formed by using mixture of herbal extracts and Muskrat (Ondatra zibethicus) musk for Anti-aging}

The present invention relates to a composition made using a composite herbal composition containing musk extract and herbal extract obtained from musk rats. More specifically, 0.0001 ~ 30 wt% musk extract obtained from musk rats and rhubarb per 100 parts by weight of water. % By weight, white paper 0.001-20% by weight, Angelica 0.001-20% by weight, hwahwa 0.001--20% by weight, gold and silver coins 0.001-20% by weight, rosewood fragrance 0.001-20% by weight, Changzai 0.001-20% by weight, Uyiin 0.001-20% by weight 60 ~ 120 after adding%, green angle 0.001 ~ 20 wt%, green tea 0.001 ~ 20 wt% ^{o It} relates to a cosmetic composition consisting of a composite composition of 0.001 to 30% by weight of the herbal extract prepared after heating at C temperature for 2 to 8 hours.

Aging refers to the loss of biological function over time that life inevitably undergoes, and the aging process of the human body is a degenerative change that occurs over a period of decades. Many theories exist to explain why aging occurs, but the most common skin aging mechanisms are:

Free radicals or free radicals are produced by UV rays or external environmental factors.Inflammation occurs on the skin as a result of a series of harmful reactions caused by free radicals or free radicals. Skin aging that breaks up to form wrinkles or the like occurs. Free radicals, which are constantly produced in vivo, oxidize cellular components (DNA, RNA, enzymes, and cell membranes), leading to cell deterioration and damage, and skin aging due to destruction of cell homeostasis.

Free radicals are deeply involved in the inflammatory response that regulates the action of various cells in vivo. In addition to mitochondria and various enzymes, free radicals involved in cell damage in cells are produced in large amounts due to the immune response of macrophages, neutrophils and other immune cells in inflammatory reactions. This is because it plays an important role in removing foreign substances from infection and inflammatory reactions. In addition, it plays an important role in activating the immune system, but when excessive amounts are continuously produced and accumulated for a long time, it causes damage to cellular components.

In the aging process, the inflammatory response of NF-κB occupies a central position. Activation of NF-κB and its gene expression have been reported to be associated with aging, inflammation, and immune responses. The complex relationship between NF-κB, inflammatory and oxidative stress is regulated by free radical status.

It is now clear that the inflammatory response is closely related to the aging process. Free radicals result in the activation of NF-κB, and activated NF-κB plays a major role in inflammatory reactions and oxidative stress, affecting aging. In addition, damage to various tissues caused by free radicals in the aging process leads to inflammatory cytokine expression and activation of inflammatory cells, thereby continuously amplifying the inflammatory response. Eventually, the accumulated free radicals cause amplification of the inflammatory response, leading to loss of homeostasis of cells, leading to aging.

Many studies and findings show how significant free radicals and inflammation can affect aging. In particular, the inflammatory response by NF-κB is a key mechanism of aging.

Therefore, in order to obtain a substantial effect as an anti-aging substance, it must be a substance having a free radical scavenging ability and an NF-κB inhibitory effect.

Collagen fibers in the skin, along with elastin fibers, form crosslinks inside the dermis. As you age, collagen fibers decrease, resulting in a sharp decrease in the elasticity of the skin and furthermore depression. Therefore, in order to delay skin aging, the effect of inhibiting collagen degradation, which maintains skin elasticity, is required, and the complex herbal extract is not only excellent in stability but also contains various beneficial drug ingredients, making it a good anti-aging target.

Musk is a natural animal fragrance, also known as Musk. Musk is a musk gland secretion from males of Moschus berezovskii Flerove, Moschus chrysogaster Hodgson, or Moschus mosschiferus Linne. have.

The effect of musk is well known for its effects on herbaceous trees and synonyms. The main ingredient of musk is colorless oily liquid muscone, and musk has been used as a medicinal herb, excitement and sedative since ancient times. In particular, it was used as a standing medicine for strokes and general paralysis because of the congestion.

The musk pharmacological action has been confirmed to have various effects such as anti-inflammatory, antibacterial, action on blood circulation system, and action on testosterone. However, despite the above advantages, musk deer, which is the main producer of musk, are designated as endangered and musk cats are identified as the main mediator of SARS, so they are largely captured and disposed of in China, making it virtually impossible to breed. So, Muskrat Musk, the only one collected from Muskrat, has emerged as a new natural substance to replace roe deer, but its availability is not easy, its price is high, and High antioxidant and anti-aging effects are not enough to be used alone as a cosmetic composition is insufficient.

Rheum Palmatum is native to Siberia due to the roots and roots of perennial herbaceous plants, Medicinal rhubarb. In oriental medicine, it is used as a medicine for health and branch offices, and in Dongbobogam, it is said to loosen the blood, cure menstrual irregularities, tumors, inflammation and get rid of constipation. Efficacy is to treat constipation by defecation and astringent effect. It has an inhibitory effect on staphylococcus aureus and fungi, promotes secretion of digestive fluids such as bile, promotes diarrhea, interpretation and digestion, and lowers serum cholesterol. Turn on.

White paper (Angelica Dahurica) is a perennial herbaceous plant in the family Apiaceae. It has the effect of enhancing breathing and raising blood pressure, and has antibacterial and coronary dilation. It is used to treat headaches, toothaches, and other pains.

Angelica gigas Nakai uses roots as a drug, which contains milk and has a strong aroma. The main ingredients are essential oils, sucrose and vitamin E. It is a representative blood donor, mainly used for women's diseases and postpartum recovery of pregnant women. In addition, it is used for severe cough and swelling.

Trifolium (Trichosanthes Kirilowii Maxim) is the root of the perennial perennial herbaceous skyweed. It has the effect of lowering the fever and producing the essence, so it is used for fever, beef, jaundice and carbuncles. Inflammation of the rash caused by the boil and the skin torn down, so as to facilitate the discharge of pus.

Lonicerae Flos is a perennial vine plant of Indongaceae. Its effects are to lower heat, detoxify, inflame, and discharge boil pus. It is generally reported to have antifungal, antiviral and antifungal action.

Rosewood incense (Ptero carpus Santalinus L.) is a scent of finely carved rosewood that is used as a fire or as a medicine. Efficacy in rubbing the boil and controlling gold.

Chang'anza (Xanthium strumarium) refers to the fruit of the dog, which is used for fever, sweating and headache. The taste is hot and bitter, the nature is warm, sinusitis is clogged nose and head hurts, it is used for runny nose and itching skin.

Coix lachrymajobi L. refers to the yulmu seed, its taste is sweet and its mildness is one of the medicinal herbs frequently used as a beauty prescription. It protects the lungs, lowers the heat and removes moisture, which helps with beauty.

Cervus Nippon Nippon Temminck is the hardened keratin of antlers. Deer antler is collected during the growth phase, and the dead skin is soft and hairy, but the rusted skin is hard and dead. It is effective for keeping the function of the liver and removing the spear and poison of the skin.

Green tea (Thea chinensis) is effective in sedation and acne skin cleansing. In particular, catechins are excellent for preventing skin aging. It also contains large amounts of vitamin C, which helps to whiten the skin and helps to treat and prevent acne by inhibiting germ propagation and sterilization.

Therefore, the present inventors confirmed that the extract containing musk and conventional herbal ingredients from musk rats has excellent free radical scavenging ability, NF-κB inhibitory effect, moisturizing and collagen biosynthesis efficacy, and completed the present invention. It became.

Free radicals or free radicals are produced by endogenous or exogenous factors, and inflammation occurs on the skin as a result of a series of harmful reactions caused by free radicals or free radicals. It is intended to improve skin aging caused by the formation of wrinkles and the like.

The present invention is to solve the above problems, musk extract obtained from muskrat and rhubarb, white paper, donkey, cheonhwabun, gold and silver, rosewood, Changzai, Uiin, green tea, antioxidant, characterized in that it comprises green tea, An anti-aging complex herbal composition having a moisturizing and collagen biosynthetic effect and a cosmetic composition comprising the same are provided.

The herbal extracts are excellent free radical scavenging activity, NF-κB inhibitory effect, moisturizing and collagen fiber biosynthesis effect is excellent. Therefore, the cosmetic composition comprising the same shows an effect of preventing and improving skin aging.

The present invention is 0.0001-30% by weight of musk extract and 0.001-20% by weight of rhubarb per 100 parts by weight of water, 0.001-20% by weight of white paper, 0.001-20% by weight of Angelica, 0.001-20% by weight of cheonhwa, 0.001 ~ 20 to 20% by weight, rosewood fragrance 0.001 to 20% by weight, Changiza 0.001 to 20% by weight, Uuiin 0.001 to 20% by weight, green angle 0.001 to 20% by weight, green tea 0.001 to 20% by weight, then 60 to 120 ^{o It} provides a composite herbal composition containing 0.001-30% by weight of the herbal extract prepared after heating at C temperature for 2-8 hours.

In the composite herbal composition of the present invention, musk is collected from muskrat, and the musk collected is inferior in preservation, and is preferably prepared by diluting to 0.0001% to 30% in a solvent such as IPM (Iso Propyl Myristate). It is characterized by.

Herbal medicines such as rhubarb, white paper, donkey, natural flower, gold and silver, rosewood, changja, uiyiin, green tea, green tea and the like may be contained in the composition in various forms. For example, the herbal medicine may be completely dried and then finely powdered, or extracted with a solvent to be included in the composition. As a solvent, water, ethanol, ethyl acetate, butanol, Butylen Glycol, Glycerin, IPM and the like can be used. Extraction methods for extracting the herbal medicines of the present invention include a cold leaching method for leaching at a relatively low temperature for a predetermined time, a warm leaching method for leaching at 35-45 ^o C, a heat extraction method for leaching at 60-120 ^o C, percolater ), Distillation cooling extraction, etc. may be used, but is not limited thereto.

The extracts of the herbal medicines may be extracted individually by a conventional method, or all at once after mixing the herbal medicines. If necessary, it can be concentrated and powdered.

In order to achieve the another object of the present invention, the present invention provides a cosmetic composition containing the composite herbal composition of the present invention.

In the cosmetic composition of the present invention, the composition may further contain cosmetic or food acceptable ingredients in addition to the herbal ingredients. For example, binders, lubricants, disintegrants, excipients, solubilizers, stabilizers, bases, lubricants, preservatives, wetting agents, emulsifiers, suspending agents, viscosity regulators, preservatives, surfactants, antioxidants, moisturizers, fragrances, pigments, etc. It may further contain. In addition, it may further contain a moisturizing agent such as ceramide or vitamin A derivatives such as retinyl palmitate, tocofepol, other plant extracts, and the like, which have been used. The composition of the present invention can be applied to the skin or topical, and can be prepared in various forms such as liquids, suspensions, ointments, creams, lotions, lotions, packs, as well as face washes such as soaps and face wash foams. Can be. It is also possible to make liposomes.

When the composition according to the present invention is prepared in the form of an external preparation applied to the skin, the compounding quantity of the composite herbal composition in the external preparation for skin may be 0.0001% to 30.0% by weight, preferably 0.01 to 20.0% by weight.

Hereinafter, the present invention will be described in more detail with reference to examples, but the embodiments according to the present invention can be modified in various forms and the scope of the invention is not limited thereto.

It was found that the composite herbal composition of the present invention has excellent free radical scavenging effect, an effect of inhibiting NF-kB involved in inflammatory response, moisturizing and collagen bioactivity. (See Experimental Examples 1 to 4) Therefore, the composite herbal composition of the present invention can be used to prepare functional cosmetics useful for anti-aging, anti-inflammatory, moisturizing and skin elasticity.

Example 1 Preparation of Musk Sample and Preparation of Herb Extract

Musk can be harvested from muskrat from the second year of spring, only males secrete musk, and females are needed for breeding. Normally, the musk rats are grown, and if the musk sac becomes hard from March, it is collected first, followed by 8-10 times a year by the end of September at 15 days intervals. Normally one musk rat can take three to five g / year of musk, and the musk rat must be skilled for a certain period of time.

Since the musk collected is usually too high in viscosity and inferior in shelf life, it is stored refrigerated by diluting it with a concentration of 0.0001% to 30% of episodic in Iso Propyl Myristate (CAS NO. 110-27-0, FEMA. 3556). do. Suspension may occur due to low viscosity during refrigeration, but use after removing suspension by standing at room temperature for 5-6 hours.

The herbal extracts are 20g rhubarb, 20g white paper, 20g Angelica 20g, 20g ginseng powder, 10g gold, silver ginseng, 10g redwood fragrance, 10g, Changiza 10g, 20g Euiin, 10g green tea, 10g green tea, and put 1000ml of water in the ground and heated 80 ^o Extracted from C for about 3 hours. Extracts Whatman NO. 1 filter paper filtered to obtain the extract.

5% musk sample and 95% herbal extract prepared as described above were mixed to obtain a composite herbal composition. This composition was called [J30] and was subjected to the next experiment.

Example 2 Cream Composition Containing J30

1) J30: 5.0 wt%

2) stearic acid: 2.0 wt%

3) stearyl alcohol: 3.0 wt%

4) Propanediol: 5.0 wt%

5) Glycol monostearate: 4.0 wt%

6) Potassium hydroxide: 0.1 wt%

7) Lecithin: 5.0 wt%

8) Cholesterol: 1.0 wt%

9) methyl paraoxybenzoate: 0.2 wt%

10) Fragrance: Appropriate

11) Purified water: Appropriate amount to be 100%

The oily component and the aqueous component were each heated to 75 ^° C., and then emulsified using a homogenizing mixer in a cream manufacturing mixer, followed by degassing, filtration and cooling.

<Example 3> Cosmetic lotion composition containing J30

1) J30: 1.0 wt%

2) propanediol: 8.0 wt%

3) Glycerin: 2.0 wt%

4) Xanthan Gum: 0.02 wt%

5) Citric acid: 0.01 wt%

6) Sodium Citrate: 0.1 wt%

7) Ethanol: 5.0 wt%

8) methyl paraoxybenzoate: 0.1 wt%

9) Polyoxyethylene Cured Castor Oil (40E.O.): 0.1 wt%

10) Fragrance: Appropriate

11) Purified water: Appropriate amount to be 100%

The components 1-6, 11, and 7-10 were melt | dissolved uniformly, respectively, and both were mixed and filtered.

Example 4 Ointment Composition Containing J30

1) J30: 5.0 wt%

2) Polyoxyethylene Cetyl Ether (30 E.O.): 2.0 wt%

3) Glycerin monostearate: 10.0 wt%

4) Liquid paraffin: 5.0 wt%

5) Cetanol: 6.0 wt%

6) Propylene Glycol: 10.0 wt%

7) methyl paraoxybenzoate: 0.1 wt%

8) Purified water: Appropriate amount to be 100%

Components 2-5 are heated, dissolved and mixed and brought to oily phase at 70 ^° C. Components 1 and 6-8 are heated, dissolved and mixed and maintained at 75 ^° C. to form an aqueous phase. The oil phase and the water phase were mixed and emulsified and prepared by cooling to 30 ^° C.

Example 5 Bath Composition Containing J30

1) J30: 1.0 wt%

2) Sodium bicarbonate: 50.0 wt%

3) Fragrance: 0.25 wt%

4) Anhydrous sodium sulfate: 44.7 wt%

1-4 components were prepared by mixing uniformly.

Experimental Example 1 Free Radical Scavenging Activity

The method used to measure the antioxidant power was 1,1-diphenyl-2-pycryl-hydrazyl (DPPH) method to prepare J30 prepared in the above example. Dilute to ethanol by concentration (0, 1, 5, 10, 50, 100 µg / mL), and put 10 µl into 96-well plates, and add DPPH prepared in 5 mM ethanol solution so that the total volume is 200 µl. After leaving for 30 minutes at, the absorbance at 517 nm was measured by ELISA Reader to determine free radical scavenging activity according to the following formula. At this time, purified water was used as a control group, and vitamin C, which is widely known to have excellent antioxidant power as a positive control group, and the complex herbal composition (J30) showed antioxidant activity in a concentration-dependent manner, and was superior to vitamin C. (Figure 1)

 Free radical scavenging rate (%)

        = (Absorbance of control group-absorbance of experimental group) / absorbance of control group] X 100

[Table 1] Free radical scavenging rate

Concentration Free radical scavenging rate (%) J30 Vitamin c 0ug / ml 0 0 1ug / ml 10 0 5ug / ml 48 4 10ug / ml 97 9 50ug / ml 98 46 100ug / ml 99 90

Experimental Example 2 NF-kB Assay

To analyze NF-kB, the most important factor in cell inflammation, and to analyze many samples at low cost, an assay using Luciferase was established. This method has a short experimental step, so the data is highly reproducible and many sample materials can be processed simultaneously. As the cell line, NIH3T3-luc cell line of Panomics was used. The preliminary experiments confirmed the expression of NF-kB and the inhibition by each candidate. About 12-17 times of luciferase (NF-kB) was induced by the induction source (TNF-alpha), and it was confirmed that the experimental reproducibility was excellent.

NIH3T3 from Panomics (Rev A 060820 Stable Cell Line: NIH3T3 / NFkB-luc 1); Stable Cell Line: NIH3T3 / NFkB-luc was used. The NIH3T3 / NFkB-luc cell line is a system designed to identify cell-based NFkB transcription factors, a cell line in which pNFkB-luc (Panomics P / N LR0051) and pHyg vectors are established through simultaneous transfection. Both of these are reporter genes. It is selected using hygromycin separation method using the property of pHyg. This method inserts the luciferase gene into the cell chromosome. Each medium production method is as follows.

[Table 2] Badge Composition Table

Put 15 mL of pre-warmed Growth medium in a 100-mm culture dish or T-75 flask, and add the cell line to the prepared culture dish to disperse the cell line well. Place the culture dish in a humidified 37 ^° C. (5% CO ₂ ) incubator and change the medium every 2-3 days. Once the 90% confluency is reached (usually within 1 week), create a stock to cryopreserve and continue incubating. Cells are cultured according to passage method to preserve cell lines.

NF-kB measurement is performed as follows. Dispense the cells into a 12-well plate at 5 x 10 ⁵ / well level in Initial Growth Media, and incubate for 24 hours in 5% CO ₂ (37 ^o C), which is moistened to attach the cell lines to the bottom. Replace with fresh medium that does not contain Serum. After incubation in a 5% CO ₂ (37 ^o C) incubator for about 8 hours, the medium is removed and 100ul lysis buffer is added to each well to measure Luciferase activity. For the measurement method, please refer to the Promega manual. (Promega P / N E1500)

NF-kB quantification using 96-Well was performed after trypsin treatment of cell lines in log-growth phase, adjusted to 5 x 10 ⁴ cells / 100ul, and placed in 96-well plates. Put it together. Incubate overnight in a 5% CO ² incubator set at 37 ^o C, treat NF-kB-inducing herbs (e.g. TNF-alpha at a 15 ul volume level, remove media and remove 50 ul lysis buffer (Promega P / N E1500). Put each well in the well and incubate for 2 minutes at room temperature with lysis buffer, and make sure that Lysis Buffer is sufficiently dissolved .. Freeze rapidly at 0 ^o C, dissolve at room temperature and mix solution 2 ~ 3 times. 20 ul of each lysate was transferred to a new 96-well, 20 ul of luciferase substrate was added to each well and mixed 2-3 times, and measured using a Luminometer.

The quantitative results of NF-kB (Luciferase assay) were compared with the appropriate dilutions of the complex herbal composition (J30) and commercially available bark skin (bark skin) and cyclosporine (CsA). The induction rate by induction source was 19.44 times, and complex herbal composition (J30) showed high NF-kB inhibition rate. They have a higher inhibition rate than cyclosporin, and show similar inhibition rates compared to bark skins known to have high anti-inflammatory properties. (Figure 2)

TABLE 3 NF-kB inhibition rate

division J30 CsA Neck skin Control group 0.56 6.66 12.17 7.79 19.45 0.14 7.64 15.07 9.57 19.45

Experimental Example 3 Skin Moisturizing Evaluation

The sample was prepared at 5% concentration using purified water and then used as an evaluation sample. As a control, purified water was used, and the equipment used was Corneometer CM820 (manufacturer: COURAGE + KHAZAKA Germany). The measurement conditions were 0.05ml / on the left (purified water) and right (epic) samples of 10 subjects in the constant temperature and humidity room maintaining the room temperature 20-25 ° C and relative humidity 45-55%. Drop the amount of circle into a 40mm circle and measure it using the probe of the corneometer. Press the corneometer probe closely to the surface of the measuring part and press it lightly. The value will be displayed. After the measurement, measure by wiping the probe surface with tissue. Note that the measurement should always be done with the same force. The measurement was carried out for 40 minutes, and the measurement method was taken three times at each measurement, and the average value was taken.

The formula for changing the moisturizing power is as follows.

Calculation: ((B-A) / A) * 100 (%)

A: Moisturizing power before sample processing B: Moisturizing power of sample

It can be seen that the moisturizing effect is superior to the purified water control group (Fig. 3).

[Table 4] Moisturizing effect

division J30 Purified water 3 minutes later 87.75 69.16 5 minutes later 55.88 50.93 10 minutes later 39.22 26.64 15 minutes later 37.25 22.43 20 minutes later 33.33 19.16 30 minutes later 28.43 14.49 40 minutes later 19.61 9.35

Experimental Example 4 Human Fibroblast Collagen Synthesis Efficacy

Inoculated in 10% fetal bovine serum is a 96 well plate in DMEM medium containing the Human fibroblast at a concentration of 5,000 cells / well and 37 ℃, 5%, CO ₂ Cultured under conditions. 0.01%, 0.1% of the composite herbal composition (J30) of Example 1 was added to the medium, respectively, and cultured for 1 day. In addition, as a control, the medium without J30 was incubated together for 1 day.

Cultures were collected from each other and collagen production was measured using a collagen biosynthesis measuring instrument (Japan Takara Shuzo Co., Ltd Catalog # MK101). The collagen biosynthesis measuring instrument uses an antibody of collagen fibers to bind the collagen fibers, and then binds the chromophores showing a specific color to the bound sites, and measures the degree of color represented by the chromophores at 450 nm to measure biosynthetic collagen fibers. The principle was to use. (Figure 4)

Table 5 Collagen Biosynthesis Rate of J30

division Concentration 0.01% weight 0.1% weight Control group Comparison Collagen
Biosynthesis rate (%) 174 197 100 67

-Control group: Contains no J30 and does not irradiate UV

Comparative group: Irradiated with ultraviolet rays without containing J30

1 is a graph showing the free radical scavenging ability of the composite herbal composition J30. The free radical scavenging rate increases in proportion to the concentration of the composite herbal composition.

Figure 2 is a graph showing the NF-kB inhibitory effect of the composite herbal composition (J30), CsA is cyclosporin, the control group was administered induction source, Y-axis fold is the NF-kB production amount of the negative control group not treated with induction source It is calculated as 1 fold.

3 is a graph showing the moisturizing increase rate of the composite herbal composition J30 (5%) compared to purified water.

4 is a test for collagen biosynthesis efficacy in Human Fibroblast with respect to the experimental group treated with J30 and the control group not irradiated with UV without treatment with J30, J30 is effective in collagen production, wrinkle generation Since the main factor is due to the decrease of collagen fiber, the increase of collagen fiber means that it can prevent wrinkles caused by aging, especially photoaging.

Claims (5)

Anti-aging or improvement herbal composition containing musk extract from musk rat and rhubarb, white paper, donkey, cheonhwa powder, gold and silver, rosewood, changja, uiyiin, green tea, green tea The method of claim 1, Composition comprising the medicinal herbs in the form of extracts The method of claim 1, Cosmetic composition having a content of 0.001 to 30% by weight based on the total weight of the composite herbal composition The method of claim 3, A composition further comprising at least one selected from the group consisting of alcohols, oils, surfactants, fatty acids, silicones, humectants, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants and perfumes. The method of claim 3, The composition consists of one formulation selected from the group consisting of lotion, essence, lotion, cream, pack, gel, ointment, patch and spray.

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