KR102200127B1 - Composition for hair growth containing an extract derived from a petiole of Green tea - Google Patents

Abstract

The present invention relates to a composition containing an extract derived from green tea leaf stalk that is safe for the skin, reduces hair loss, promotes hair growth, and has high stability.

Description

Composition for hair growth containing an extract derived from a petiole of Green tea}

The present invention relates to a composition containing an extract derived from green tea leaf stalks that are safe for the skin, reduce hair loss, promote hair growth, and have high stability.

There are about 100,000 to 150,000 hairs in the human body, and each hair grows and degenerates repeatedly through different cycles. Hair grows in the growth phase (Anagen), the degenerative phase in which the metabolic process is slowed while maintaining the shape of the hair after the growth phase (Catagen), and the telogen phase in which the hair follicles are pushed upward as the hair follicles are gradually atrophy and the hair follicles become smaller. The cycle of three stages of) is repeated, and the cycle and lifespan of such hair may change according to various conditions such as nutritional status, medical history, heredity, constitution, hormone secretion or aging.

Hair loss is a problem that occurs through natural processes or is chemically promoted by the use of certain therapeutic drugs designed to alleviate symptoms such as cancer. This hair loss is accompanied by a lack of hair regrowth leading to partial or complete baldness.

Recently, as the number of modern people with alopecia due to environmental pollution, stress, diet, fatigue, and incorrect eating habits is increasing, the number of men or women suffering serious mental pain is increasing.

Meanwhile, currently representative hair loss inhibitors include Propecia and Minoxidil, which have been approved by the US FDA. These drugs are clinically clear, but not only have side effects such as sexual dysfunction and skin irritation, but also have limited or unusable disadvantages for women. In recent years, development of natural ingredients that can be used without restriction on gender and safe, along with a hair growth agent of a new mechanism that can compensate for the shortcomings of these drugs, has been actively made.

Domestic Publication No. 2013-0108854 (published on October 7, 2013)

Accordingly, the present inventors have found that the extract of green tea leaf sack, which has been discarded without being used in the past, can provide excellent hair loss prevention and hair growth promoting effects without irritation to the skin, and completed the present invention.

Accordingly, an object of the present invention is to provide a composition exhibiting excellent hair loss prevention and hair growth promoting effect by using an extract derived from green tea leaf stalk.

In order to achieve the above object, the present invention provides a composition for external application for skin for preventing hair loss or promoting hair growth, comprising an extract derived from a green tea leaf stalk as an active ingredient.

The present invention has an excellent use value in terms of utilization of waste resources by using green tea leaf sacks that have not been previously utilized and have been discarded. In addition, the composition of the present invention contains an extract derived from a green tea leaf stalk, so that it is safe for the skin and has high formulation stability, suppresses stress and strengthens the hair roots to prevent hair loss while promoting hair growth.

The composition of the present invention may provide an excellent hair loss prevention or hair growth promoting effect by containing the green tea leaf stalk extract as an active ingredient.

Green tea has generally been made using the leaves of the tea tree (Camellia sinensis). However, in the present invention, there is no limitation on the type and number of cultivation days of the tea tree, but the used site is characterized in that it is a petiole. In addition, the petiole used in the present invention may be dried or not dried.

The petiole of green tea used in the present invention is a part also called a petiole, and the vascular bundle in the petiole serves to connect the vascular bundle of the stem with the vascular bundle of the leaf body, and conventionally, the leaf was collected from the tea tree and discarded. However, as a result of analyzing the green tea leaf sacks by the present inventors, it could be predicted that there will be a difference in the effect provided from the green tea leaves because there was a large difference in the type and content of the components contained therein.

In addition, by manufacturing extracts derived from green tea leaf stalks that were not previously utilized and discarded, and applying them to products, it becomes possible to use them as functional materials and applied products of high added value, so the present invention has excellent use in terms of utilization of waste resources. It's worth it.

The extract derived from the green tea leaf sack used in the present invention may be prepared by a method known in the art, and the method is not particularly limited, but a method of extracting the water-soluble component contained in the green tea leaf sack is preferably used.

Specifically, hot water (purified water with a temperature of 80 to 90°C) 5 times the weight of the green tea leaf sacks was added to the dried green tea leaf sacks, heated and stirred for 2 hours while maintaining the temperature, cooled to room temperature, and filtered first. Only water-soluble components are separated. Ethanol equivalent to twice the total weight of the filtrate is added while stirring slowly, chlorophyll in the supernatant is removed, and only the precipitated is collected and dried with hot air. It maintains 30~40℃ when drying with hot air to prevent thermal degeneration. In this way, the extract derived from green tea leaf sack used in the present invention can be prepared.

The extract derived from green tea leaf sack prepared in this way is composed of a general green tea leaf extract containing 22.1% catechin and 4.61% caffeine, as the main ingredient is glycoprotein, 0.90% catechin, and 0.12% caffeine. The components are different, and the effects exhibited by them are also different from each other.

The composition of the present invention may contain an extract derived from green tea leaf sack in an amount of 0.001 to 20% by weight based on the total weight of the composition. When the content of the active ingredient is less than 0.001% by weight, the efficacy and effect are weak, and when it exceeds 20% by weight, it is difficult to expect an increase in the effect according to the amount of raw material used.

The extract derived from green tea leaf stalk used in the present invention effectively inhibits the acceleration of hair follicles from the growth phase to the degenerative phase than in the normal state due to stress, thereby suppressing the hair loss phenomenon (degeneration phase transition) caused by stress, and strengthening the hair roots to promote hair growth. Provided, it is safe for the skin and has high stability, which is desirable for industrial use.

The composition for promoting hair growth of the present invention is not particularly limited in formulation, but it is a cosmetic composition having a formulation such as hair tonic, hair nourishing lotion, scalp treatment, hair treatment, hair rinse, hair shampoo, and hair lotion. I can.

In addition, in each formulation, other ingredients other than the above-described essential ingredients may be appropriately selected and blended by a person skilled in the art without difficulty depending on the type or purpose of use of other external preparations.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples, but the present invention is not limited to these examples.

[Example 1] Preparation of extract derived from green tea leaf sack

Purified water having a temperature of 80-90°C was added to the dried green tea leaf sack (Jangwon, an agricultural corporation) in an amount 5 times the weight of the green tea leaf sack, and heated and stirred for 2 hours while maintaining the temperature. After cooling to room temperature, Adventec No. Only water-soluble components were separated by primary filtration using 131 (Adventec MFS, Inc., Japan). Ethanol equivalent to twice the total weight of the filtrate was added while stirring slowly, the chlorophyll of the supernatant was removed, and only the precipitated was collected and dried with hot air while maintaining a temperature of 30 to 40°C. It was obtained in the form of white powder of 1 to 3% by weight of a green tea leaf sack.

[Comparative Example 1] Preparation of green tea leaf extract

Purified water having a temperature of 80 to 90°C was added to a commercially available green tea leaf (Amore Pacific Co., Ltd.) in an amount of 5 times the weight of the green tea leaf, and heated and stirred for 2 hours while maintaining the temperature. After cooling to room temperature, Adventec No. Only water-soluble components were separated by primary filtration using 131 (Adventec MFS, Inc., Japan). After filtration, it was concentrated using a vacuum distillation apparatus and dried by using a freeze dryer to obtain a green tea leaf extract as yellowish brown powder.

[Test Example 1] Evaluation of hair loss inhibition efficacy

Stress induces the secretion of the substance P (Substance P) at the nerve endings and causes macrophages, γδ T cells and mast cells to secrete inflammatory signaling substances (cytokines) through the substance P receptor (NK-1 receptor). ), which in turn prevents the growth of keratinocytes in the hair follicle and induces apoptosis (Arck et al., FASEB J. 2001; 15(13): 2536-2538), due to stress. In order to prevent hair loss, an antagonist to the matrix P receptor or a method of preventing degranulation of mast cells due to stress has been proposed (Am J Pathol. 2003; 162(3): 709-712).

Therefore, in this test, the effect of green tea leaf stalk-derived extract on the degree of degranulation of mast cells was measured.To examine the inhibitory effect of β-hexosaminidase on degranulation, an indicator of immediate allergic reaction, Secretion was measured.

Mast cells (RBL-2H3 cells) were suspended in DMEM containing 10% FBS, and then cells were dispensed in a 48-well plate (Corning, NY, USA) in an amount of 5×10 ⁵ cells/ml. Then, it was sensitized with anti-DNP IgE (30ng/ml) and incubated for 24 hours in a 37°C 5% CO ₂ incubator. The cells in each well were washed twice with Siraganian buffer (119mM NaCl, 5mM KCl, 0.4mM MgCl ₂ , 25mM PIPES, 40mM NaOH, pH 7.2), and then 5.6mM glucose, 1mM CaCl ₂ and Siraganion buffer containing 0.1% BSA was added, and the green tea leaf stalk extract of Example 1 and the green tea leaf extract of Comparative Example 1 were mixed at each concentration (0, 50, 100 ppm) in a 37° C. 5% CO ₂ incubator for 1 hour. After incubation in DNP-HSA (10 ㎍ / ㎖) was treated for 1 hour, and left in an ice bath (ice bath) for 10 minutes to terminate the reaction. Transfer 20 μl of the supernatant to a 96-well plate, add 80 μl of the substrate buffer (4-p-nitrophenyl-N-acetyl-bD-glucosamined 1mM, sodium citrate 0.05M, pH 4.5), and incubate at 37°C for 30 minutes. The reaction was terminated by adding 200 μl of a stop solution (0.1M Na ₂ CO ₃ /NaHCO ₃ , pH 10.0) per well. Absorbance was measured at 405 nm using a microplate reader (Molecular Devices Co. Ltd., USA). The inhibition rate (%) was calculated by Equation 1 below as absorbance values of the green tea leaf stalk extract and the green tea leaf extract treatment group and the control group.

C: cells (+), DNP-HSA (+), test sample (-)

T: cells (+), DNP-HSA (+), test sample (+)

The measurement results are shown in Table 1 below.

Test substance Degree of degranulation of mast cells Control (no addition) 100 Example 1 50ppm 86 100ppm 81 Comparative Example 1 50ppm 95 100ppm 93

Referring to Table 1, when Comparative Example 1, which is a green tea leaf extract, was treated, the degree of degranulation of mast cells was suppressed, but the degree was not large, whereas when Example 1, which was a green tea leaf extract, was treated, mast cells were It can be seen that it effectively inhibits degranulation.

Through this, it can be seen that the green tea leaf stalk extract used in the present invention blocks the production of intracellular inflammatory substances, and thus can effectively act to prevent hair loss due to stress.

[Test Example 2] Skin stability evaluation

In order to find out the degree to which the green tea leaf stalk extract used in the present invention irritates the skin, a primary human skin irritation test was conducted.

The test was conducted twice, and in the first round, 34 healthy adult females and 6 males, a total of 40 (average age 35.1 years), and in the second round, 28 females and 2 males, a total of 30 (average 30.1 years of age).

Specifically, a solution made by dissolving the green tea leaf stalk extract obtained in Example 1 in water to a concentration of 10% was evenly applied to the skin of the back area in an amount of 20 mg/cm ² .

After application, the patch was attached to the area, and after 24 hours, the patch was removed, and the first reading was performed 30 minutes later, and the second reading was performed after 24 hours. If it was 1+ or higher, it was read as positive and the last reading was considered definitive. In order to find out the intensity of skin irritation of the sample, weights are given according to the degree of positive skin reaction (-, +, ++, +++, ++++ E　values of 0, 1, 2, 3, 4 respectively Is given) to obtain the average reactivity of the skin. Specific criteria are shown in Table 2 below. In addition, the calculation of the average skin reactivity was calculated by Equation 2 below, and the criteria for determining the result accordingly are shown in Table 3.

Criteria reaction weight Criterion of judgment - 0.0 : No reaction + 1.0 : Faint erythema ++ 2.0 : Erythema, swelling, and papules with distinct but weak boundaries +++ 3.0 : Pronounced erythema, papules, and blisters ++++ 4.0 : Cannon

Criteria for judging results Average skin reactivity Criteria 0.0 to 0.9
1.0 to 2.9
3.0 to 4.9
5.0 or higher No irritation
Light stimulation
Heavy stimulation
Strong stimulation

As a result of the measurement, the average skin reactivity after using the green tea leaf stalk extract was between 0.0 and 0.9, and it was finally determined that there was no irritation to the skin.

Hereinafter, an example of the formulation of the composition according to the present invention will be described, but the pharmaceutical composition and the cosmetic composition can be applied in various formulations, and this is not intended to limit the present invention, but is intended to be described in detail.

[Formulation Example 1] Hair Tonic

Hair tonic was prepared by a conventional method according to the composition shown in Table 4 below.

Raw material name Content (% by weight) ethanol 50 menthol 0.02 glycerin 3 Salicylic acid 0.05 Green Tea Leaf Sack Extract 1.0 Preservatives, flavors and colors Appropriate amount Purified water Balance

[Formulation Example 2] Hair Lotion

A hair lotion was prepared in a conventional manner according to the composition shown in Table 5 below.

Raw material name Content (% by weight) Cetostearyl alcohol 2.0 EDTA 2Na 0.2 Hydroxyethylcellulose 0.5 Mineral oil 5.0 Green Tea Leaf Sack Extract 1.0 Preservatives, flavors and colors Appropriate amount Purified water Balance

[Formulation Example 3] Hair nourishing lotion

According to the composition shown in Table 6 below, hair nutrient cosmetic water was prepared in a conventional manner.

ingredient Weight ratio (%) Beeswax 4.0 Polysorbate 60 1.5 Sorbitansquioleate 0.7 Mineral oil 10.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Green Tea Leaf Sack Extract 1.0 antiseptic Appropriate amount Flavoring and coloring Appropriate amount Purified water Balance (to 100)

[Formulation Example 4] Hair Shampoo

A hair shampoo was prepared by a conventional method according to the composition shown in Table 7 below.

ingredient weight% Purified water Balance (To 100) Green Tea Leaf Sack Extract 1.0 Sodium lauryl sulfite 36.0 Cocamidopropylbetaine 8.0 Palmitidine malate 2.0 Glycol stearate 1.5 Polyquaternium 10 0.5 Cyctic Acid 0.1 glycerin 2.0 Preservatives, colors and flavors Appropriate amount

[Formulation Example 5] Hair conditioner

A hair rinse was prepared by a conventional method according to the composition shown in Table 8 below.

ingredient weight% Purified water Balance (To 100) Green Tea Leaf Sack Extract 1.0 Propylene glycol 2.0 Cetyltrimethylammonium chloride 1.0 Cetyl alcohol 3.0 Stearyl alcohol 3.0 Mineral oil 0.5 Cyctic Acid 0.2 Polydimethylsiloxane 1.0 Preservatives, colors and flavors Appropriate amount

Claims (8)

A composition for external application for skin for anti-inflammatory containing an extract derived from green tea leaf sack from which chlorophyll has been removed as an active ingredient. The composition of claim 1, wherein the content of the extract is 0.001 to 20% by weight based on the total weight of the composition. The composition for external application for skin for anti-inflammatory according to claim 1, wherein the extract is derived from a water-soluble component obtained by extracting green tea leaf sacks with hot water. The composition for external application for skin for anti-inflammatory according to claim 3, wherein the hot water is purified water heated to a temperature of 80 to 90°C. delete The composition for external application for skin according to claim 1, wherein the extract contains catechin and caffeine in a weight ratio of 0.90:0.12. According to claim 1, wherein the composition is characterized in that blocking the production of inflammatory substances in mast cells, anti-inflammatory composition for external application for skin. 8. The composition for external application for skin according to claim 7, wherein the inflammatory substance is an inflammatory cytokine.