KR102052517B1 - Composition for improving condition of hair and scalp containing Panax ginseng polysaccharides and Green tea polysaccharides - Google Patents

Abstract

The present invention relates to a composition for improving hair or scalp condition containing ginseng polysaccharide and green tea polysaccharide, and more specifically, by using a mixture of ginseng polysaccharide and green tea polysaccharide to prevent white hair and hair loss, and to reduce irritation to the scalp. It suppresses dandruff, scalp inflammation and the like to improve the overall hair condition and the composition is excellent for the cleansing and soothing effect on the scalp.

Description

Composition for improving condition of hair and scalp containing Panax ginseng polysaccharides and Green tea polysaccharides}

The present invention relates to a composition for improving hair or scalp condition containing ginseng polysaccharide and green tea polysaccharide, and more specifically, by using a mixture of ginseng polysaccharide and green tea polysaccharide to prevent white hair and hair loss, and to reduce irritation to the scalp. It suppresses dandruff, scalp inflammation and the like to improve the overall hair condition and the composition is excellent for the cleansing and soothing effect on the scalp.

The scalp is composed of epidermis, dermis and subcutaneous tissue, with the outermost tissue being the epidermis. The epidermis functions to protect the sensitive tissues of the body from damage. However, various physical and chemical external stimuli such as external temperature, humidity, pollutants, stress, and lack of influence can cause the scalp to lose function and further affect hair loss.

In addition, in modern society that emphasizes beauty, many people of all ages, including men and women, use hair dye and perm to satisfy individuality expression needs. However, some sensitive scalp chemicals such as staining, perming, irritation and inflammation such as itching and itching of the scalp, which leads to environmental hair loss.

As such, it is possible to protect the scalp from stimulation, stress, and chemical treatment from the environment due to various causes, promote hair growth while preventing hair loss, and continue research on eco-friendly and harmless ingredients to the environment and humans. It is becoming.

Therefore, the present inventors found that when the ginseng polysaccharide derived from ginseng and the green tea polysaccharide derived from green tea are used at the same time, the effects of white hair and hair loss prevention and dandruff suppression are superior to those of using these substances by synergistic effects, respectively. The present invention has been completed.

Therefore, an object of the present invention is to provide a composition exhibiting excellent hair or scalp condition improvement effect by simultaneously using ginseng polysaccharide and green tea polysaccharide.

In order to achieve the above object, the present invention provides a skin external composition or pharmaceutical composition for improving the hair or scalp state containing ginseng polysaccharide and green tea polysaccharide as an active ingredient.

The composition of the present invention contains ginseng polysaccharides and green tea polysaccharides to prevent white hair and hair loss, to relieve scalp irritation, to prevent scalp itching and dandruff, as well as to make hair softer and more shiny.

The composition of the present invention by providing a ginseng polysaccharide and a green tea polysaccharide as an active ingredient, provides an excellent hair or scalp condition improvement effect.

The ginseng (Panax ginseng CA Meyer) polysaccharide used in the present invention is a pectin-like substance having a molecular weight of 34,600, and contains about 60% of galacturonic acid. Galactose and the like constitute a side chain. Ginseng used in the present invention is a plant belonging to the genus Ogapi and ginseng, ginseng in the present invention can be used without limitation in its kind and annual root, can be used both ginseng and dried. In addition, ginseng in the present invention can be used without limitation in the area, specifically, the root of ginseng can be used.

In addition, the green tea polysaccharide used in the present invention is derived from green tea, and unlike the polysaccharides found in other plants, it is an acidic polysaccharide group, and is produced by combining sugar components and amino acids made through photosynthesis. Green tea in the present invention can be used without limitation in the type and cultivation days, it is possible to use both dried or not dried green tea. In addition, the green tea in the present invention can be used without limitation, specifically, the leaves of green tea can be used.

Ginseng polysaccharide and green tea polysaccharide used in the present invention may be prepared by a method known in the art, the method is not particularly limited.

The ginseng polysaccharide and green tea polysaccharide used in the present invention include water; At least one organic solvent selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform; Alternatively, using the mixed solvent of the organic solvent and water, and immersed ginseng root extract or green tea leaf extract prepared by extracting for 3 to 24 hours at 38 ~ 42 ℃, and separating the residue and the filtrate through filtration and centrifugation It can be obtained by dropwise addition of ethanol to the separated filtrate and drying. Specifically, after preparing ginseng root or green tea leaf extract with water at 38-42 ° C., and concentrating it, removing impurities with a filter, and then adding ethanol and hot air drying, green tea polysaccharide and ginseng polysaccharide can be obtained. .

The composition of the present invention may contain a ginseng polysaccharide and a green tea polysaccharide in an amount of 0.001 to 10% by weight based on the total weight of the composition, respectively, preferably a ginseng polysaccharide and green tea polysaccharide to 0.005 to 9.5% by weight, respectively , 0.01-9%, 0.03-8.5%, 0.05-8%, 0.07-7.5%, 0.09-7%, 0.1-6.5%, 0.3-6%, 0.5-5.5% or 0.7 It may be contained in an amount of ˜5 wt%.

In the present invention, ginseng polysaccharide and green tea polysaccharide are used by mixing in the range of 0.1 to 10: 1 by weight. In addition, in order to maximize the synergistic effect of the combination of the two components, preferably used to mix the ginseng polysaccharide and green tea polysaccharide in the range of 0.5 to 9.5: 1, 1 to 9: 1 or 1.5 to 8.5: 1 by weight ratio Can be.

The composition of the present invention can be used as a composition for preventing dandruff, which effectively discharges toxins accumulated in hair and scalp to cleanse the scalp, inhibit the growth and growth of dandruff bacteria, and prevent scalp inflammatory reactions, It has an excellent antioxidant effect that inhibits the production and action, so it can provide the effect of calming and strengthening the scalp and strengthening its natural defense.

The composition of the present invention can be used as a composition for hair growth, which can promote the growth of hair and prevent hair loss by promoting the transition of the resting hair cycle to the growing hair cycle.

The composition of the present invention can be used as a topical anti-skin composition for white hair, which can significantly increase the expression of MITF in melanocytes, inhibit white hair and promote the induction of black hair.

The composition of the present invention can stabilize sensitive, irritant or reactive scalp by reducing irritation phenomena such as itching and stinging of the scalp and alleviating scalp inflammation. It also provides a conditioning effect that softens and smoothes rough and damaged hair, thereby improving the health of the hair.

The composition of the present invention may be formulated as an external composition for skin, in particular a cosmetic composition, and may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the compositions of the present invention may be provided in all formulations suitable for topical application, for example, emulsions obtained by dispersing the oil phase in a solution, aqueous phase, emulsions obtained by dispersing the aqueous phase in oil phase, suspensions, solids, gels, powders, It may be provided in the form of a paste, foam or aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.

In particular, the topical skin composition of the present invention is not specifically limited as a composition for hair or scalp, for example, hair tonic, hair nourishing longevity, scalp treatment, hair treatment, hair shampoo, hair rinse, hair lotion or And scalp hair combination treatments and the like.

In addition, the composition according to the present invention may include other ingredients in addition to the above-mentioned materials within a range not impairing the main effect, preferably a synergistic effect on the main effect. In addition, the composition according to the present invention may further include a moisturizer, an emulsifier, a UV absorber, a preservative, a fungicide, an antioxidant, a pH adjuster, organic and inorganic pigments, flavoring, cooling agent or limiting agent. The blending amount of the above components can be easily selected by those skilled in the art within the range that does not impair the object and effect of the present invention, the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight relative to the total weight of the composition. .

In addition, the compositions of the present invention may be formulated as pharmaceutical compositions. When the composition according to the present invention is applied to a pharmaceutical product, it may be formulated as an oral or parenteral dosage form in the form of a solid, semi-solid or liquid by adding a commercially available inorganic or organic carrier to the active ingredient used in the present invention. , The pharmaceutical composition according to the invention can be administered orally, parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous.

Formulations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups, pellets and the like. In addition, the formulation for parenteral administration includes injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated by carrying out according to a conventional method, and at this time, surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffers, suspending agents, and other commercially available auxiliaries can be suitably used. have.

The dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight, pathology and severity of the subject to be administered, the route of administration or the judgment of the prescriber. Determination of a suitable dose based on these factors is within the level of one of skill in the art and its daily dosage may be, for example, 0.1 mg / kg / day to 100 mg / kg / day, more specifically 5 mg / kg / day to 50 mg / kg. May be, but is not limited to.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Reference Example 1 Preparation of Ginseng Polysaccharide

1 kg of dried white ginseng was pulverized with a blender, and 10 liters of purified water was added thereto, stirred and extracted with 38-42 ° C. hot water for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to 1/10 the amount of purified water. To this was added dropwise ethanol in a concentrated amount of 4 times and hot air dried to obtain 65 g of ginseng polysaccharide.

Reference Example 2 Preparation of Green Tea Polysaccharide

1 kg of the first processed green tea leaves were pulverized with a blender, and then 10 liters of purified water was added thereto, stirred and extracted with hot water at 38-42 ° C. for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to 1/10 the amount of purified water. To this was added dropwise four times the concentrated ethanol and hot air dried to obtain 72g of green tea polysaccharide.

Example 1 Preparation of a Mixture of Ginseng Polysaccharide and Green Tea Polysaccharide

The ginseng polysaccharide prepared in Reference Example 1 and the green tea polysaccharide prepared in Reference Example 2 were mixed at a ratio of 1: 3.

Example 2 Preparation of Mixture of Ginseng Polysaccharide and Green Tea Polysaccharide

The ginseng polysaccharide prepared in Reference Example 1 and the green tea polysaccharide prepared in Reference Example 2 were mixed at a ratio of 1: 1.

Example 3 Preparation of Mixture of Ginseng Polysaccharide and Green Tea Polysaccharide

The ginseng polysaccharide prepared in Reference Example 1 and the green tea polysaccharide prepared in Reference Example 2 were mixed at a ratio of 3: 1.

Reference Example 1 Preparation of Formulation Example and Comparative Formulation Example

To prepare a shampoo in the composition of Table 1. Specifically, the surfactant and ethylene glycol distearate are added to purified water, heated to 80 ° C., uniformly dissolved, and then slowly cooled to 40 ° C. under stirring, and the active ingredient and preservative according to the present invention are added to the mixture. A viscosity modifier, a perfume, and a hair conditioner were added and mixed, followed by cooling to room temperature under stirring.

ingredient Formulation example Comparative formulation example One 2 3 One 2 3 Ammonium Lauryl Sulfate 10 10 10 10 10 10 Polyoxyethylene lauryl ammonium sulfate 5 5 5 5 5 5 Cocoamidopropylbetaine 2 2 2 2 2 2 Ethylene Glycol Distearate 1.5 1.5 1.5 1.5 1.5 1.5 Cocoyl Monoethanolamide 0.8 0.8 0.8 0.8 0.8 0.8 Example 1 5.0 - - - - - Example 2 - 5.0 - - - - Example 3 - - 5.0 - - - Reference Example 1 - - - 5.0 - - Reference Example 2 - - - - 5.0 - Polyquaternium-10 0.2 0.2 0.2 0.2 0.2 0.2 Blue No. 1 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 Yellow No. 4 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 Methylparaben 0.1 0.1 0.1 0.1 0.1 0.1 Spices 0.8 0.8 0.8 0.8 0.8 0.8 Citric acid 0.1 0.1 0.1 0.1 0.1 0.1 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 water to 100 to 100 to 100 to 100 to 100 to 100

[Test Example 1] dandruff reduction effect test

Sixty men aged 19 to 35 years old who had a lot of dandruff were selected and divided into six groups of 10 people. For each group, the shampoos of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 were used for one month as follows. After the dandruff reduction rate was measured.

Triturate with regular shampoo before the start of the test, and collect the dandruff accumulated for 2 days after the shampoo. It was. At this time, the accumulated dandruff was collected directly from the scalp with a vacuum suction device, to obtain a rate of dandruff reduction based on Equation 1 below and the results are shown in Table 2 below.

Dandruff reduction rate (%) Formulation Example 1 34.5 Formulation Example 2 34.2 Formulation Example 3 32.9 Comparative Formulation Example 1 30.8 Comparative Formulation Example 2 29.8 Comparative Formulation Example 3 2.5

As can be seen in Table 2, when the Comparative Formulation Example 3 containing no ginseng polysaccharide and green tea polysaccharide is used, the anti-dandruff effect is hardly exhibited. However, in the case of using Comparative Formulation Examples 1 or 2 and Formulation Examples 1 to 3 containing ginseng polysaccharide or green tea polysaccharide, dandruff may be reduced. Particularly, Formulation Examples 1 to 3 used by mixing ginseng polysaccharide and green tea polysaccharide. In the case of can be seen that due to the synergistic effect of the mixed use of these polysaccharides exhibits a remarkably excellent anti-dandruff effect.

Test Example 2 Scalp Itching Prevention Test

Sixty men and women aged 25-45 who feel relatively itchy scalp were selected and divided into six groups of 10 people. For each group, the shampoos of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 were administered once every 3 days for 2 weeks. After using it to evaluate the scalp itching effect through the following evaluation criteria and the results are shown in Table 3 below.

[Evaluation standard]

Very good-5 points

Excellent-4 points

Normal-3 points

Poor-2 points

Very bad-1 point

division Itching effect of scalp Formulation Example 1 3.3 Formulation Example 2 3.4 Formulation Example 3 3.3 Comparative Formulation Example 1 3.0 Comparative Formulation Example 2 2.8 Comparative Formulation Example 3 2.3

As can be seen in Table 3, when using Comparative Formulation Example 3 containing no ginseng polysaccharide and green tea polysaccharide, it was not good to prevent scalp itching, Comparative Formulation Example 1 containing ginseng polysaccharide or green tea polysaccharide In case of using 2, the scalp itch prevention effect is shown, but the degree is moderate. On the other hand, Formulation Examples 1 to 3 using a mixture of ginseng polysaccharides and green tea polysaccharides can be seen to exhibit a better scalp itching effect due to the synergistic effect of the mixed use of these polysaccharides.

[Test Example 3] hair follicle dermal papilla cell proliferation effect

The keratin proteins that make up hair are produced in hair root keratinocytes, which are differentiated from dermal papilla cells. In order to evaluate the activity of the dermal papilla cells of the composition, a rat immortalized dermal papilla cell (DP6) cell line was used in the present invention (Wendy Filsell, Journal of Cell Science 107, 1761-1772 (1994)). This dermal papilla cell line was cultured by microdissection from the hair roots of male PVG rat whiskers and cultured with DMEM (Dulbecco's modified Eagle's medium, Gibco BRL, Gaithersburg, MD, USA) containing 10% FBS (Fetal bovine serum). USA) was incubated for 24 hours in an incubator maintained at 5% CO ₂ , 37 ℃. DP6 was placed in a 96 well plate and incubated in a 37 ° C. incubator for 24 hours, and then Examples 1-3 and Reference Examples 1-2 were treated at concentrations of 5 ppm, 10 ppm and 20 ppm, respectively. After 24 hours of drug treatment, cell proliferation was measured using the WST-1 kit (Roche). The results are shown in Table 4 below.

division Cell proliferation (%) Untreated Control 100 Example 1 5 ppm 113 10 ppm 117 20 ppm 129 Example 2 5 ppm 112 10 ppm 117 20 ppm 127 Example 3 5 ppm 112 10 ppm 117 20 ppm 126 Reference Example 1 5 ppm 108 10 ppm 113 20 ppm 121 Reference Example 2 5 ppm 110 10 ppm 115 20 ppm 125

As can be seen in Table 4, the reference examples 1 and 2 treated with ginseng polysaccharides or green tea polysaccharides have better dermal papilla cell proliferation effects than the controls not treated with ginseng polysaccharides and green tea polysaccharides. In particular, in the case of Examples 1 to 3 using a mixture of ginseng polysaccharide and green tea polysaccharide, the growth of dermal papilla cells is more effectively increased due to the synergistic effect of the mixed use of these polysaccharides, which increases significantly in a concentration-dependent manner. You can see that.

Test Example 4 Evaluation of Potassium Ion Channel Activity Increase Effect

Hair loss treatment of minoxidil is known as mitochondrial potential potassium ion channel openers (K _ATP channel opener), a representative drug used in the treatment of androgenetic alopecia. To evaluate the mechanism of minoxidil, the treatment of toltamide (SIGMA AlDRICH, T0891), which blocks the K _ATP channel in fibroblasts constituting the dermis of the scalp, inhibits cell proliferation, and then opens potassium ion channels to allow cell proliferation. A recovering test method was used.

In order to evaluate the function of the composition as a K _ATP channel opener, in the present invention, a mouse embryonic fibroblast cell line (NIH3T3) cell line was used. The cell line is a cell line in which the fibroblast cell line isolated from NIH Swiss mouse embryo was naturally immortalized by 3T3 protocol. The cell line was incubated in DMEM (Gibco BRL, Gaithersburg, MD, USA) containing 10% FBS for 24 hours in an incubator maintained at 5% CO ₂ , 37 ℃. After NIH3T3 was placed in a 96-well plate and incubated in a 37 ° C. incubator for 24 hours, 2.5 mM tolbutamide was treated, and after 10 minutes, 10 μM of minoxidil, a positive control group, and Examples 1 to 3 and Reference Examples 1 and 2, respectively, were 2.5. The cells were treated at concentrations of ppm, 5 ppm and 10 ppm, and 48 hours after drug treatment, cell proliferation was measured using the WST-1 kit (Roche). The results are shown in Table 5 below.

division Cell proliferation (%) Untreated Control 100 Minoxidil 132 Example 1 2.5 ppm 110 5 ppm 114 10 ppm 122 Example 2 2.5 ppm 111 5 ppm 115 10 ppm 124 Example 3 2.5 ppm 111 5 ppm 116 10 ppm 125 Reference Example 1 2.5 ppm 106 5 ppm 111 10 ppm 119 Reference Example 2 2.5 ppm 106 5 ppm 112 10 ppm 118

As can be seen in Table 5, the reference examples 1 and 2 treated with ginseng polysaccharides or green tea polysaccharides may be superior to the fibroblast proliferation effect compared to the control group not treated with ginseng polysaccharides and green tea polysaccharides. . In particular, in Examples 1 to 3 using a mixture of ginseng polysaccharides and green tea polysaccharides, the growth of fibroblasts is more effectively increased due to the synergistic effect of the use of these polysaccharides, which is significantly increased in a concentration-dependent manner. You can see that.

[Test Example 5] melanin production promoting effect test

Add melan-a to 50,000 cells / well in a 24-well microtiter plate in medium containing 5% fetal bovine serum, RPMI medium, 100 IU penicillin G and 0.2 μM TPA in RPMI medium. Busy. The following day, the treated cells were treated with Examples 1 to 3, Reference Examples 1 and 2, respectively, at a final concentration of 10 ppm or 50 ppm, respectively, and treated with 0.1% DMSO as a negative control and 100 μM IBMX as a positive control. Incubated for 3 days at a temperature. After the incubation, the wells were washed with PBS, 100 μl of 1N NaOH was added, and the melanin in the cells was dissolved. The absorbance of the dissolved melanin was measured at 405 nm using a microplate reader. The result of comparing the melanin production promoting effect of ginsenoside Y with the control group is shown in Table 6 below.

division Melanin Synthesis (%) DMSO (0.1%) 100 IBMX (100 μM) 120 Example 1 10 ppm 109 50 ppm 121 Example 2 10 ppm 110 50 ppm 122 Example 3 10 ppm 110 50 ppm 124 Reference Example 1 10 ppm 105 50 ppm 117 Reference Example 2 10 ppm 106 50 ppm 119

In Table 6, the ginseng polysaccharide and the green tea polysaccharide promote melanin synthesis of melanocytes, and in particular, in Examples 1 to 3, in which the ginseng polysaccharide and the green tea polysaccharide were mixed, due to the synergistic effect of the mixed use of these polysaccharides. It can be seen that the excellent melanin production promoting effect.

Test Example 6 Effect of promoting MITF and tyrosinase expression in melanocytes

Dispense 500,000 cells / well in a 6-well microtiter plate using a 501mel cell line, DMSO 0.1% as a negative control, IBMX 100μM as a positive control, and Example 1 as a test group in each hole. To 3, Reference Examples 1 and 2, respectively, were treated with 10 ppm, and then cultured at 37 ° C. for 24 hours, 48 hours, and 72 hours to obtain proteins. The protein thus obtained was subjected to western blot using MITF and tyrosinase antibody. Protein extraction and western blot were typically performed by standard methods used by those skilled in the art. After Western blot the results are shown in Tables 7 and 8 below with a negative control of 100 and compared with this value.

Western blot using MITF antibody division 24 hours 48 hours 72 hours IBMX 119 97 212 Example 1 86 118 156 Example 2 84 114 150 Example 3 80 111 148 Reference Example 1 78 110 148 Reference Example 2 76 106 142

Western blot using tyrosinase antibody division 24 hours 48 hours 72 hours IBMX 155 147 287 Example 1 134 160 188 Example 2 135 162 191 Example 3 138 167 193 Reference Example 1 125 154 176 Reference Example 2 127 157 184

Referring to Tables 7 and 8, the ginseng polysaccharide and the green tea polysaccharide increase the expression of MITF and tyrosinase protein in melanocytes, and particularly, the effect of increasing the expression of the MITF and tyrosinase protein is increased when the ginseng polysaccharide and the green tea polysaccharide are mixed and treated. It can be confirmed that excellent.

Reference Example 2 Preparation of Formulation Examples 4-6 and Comparative Formulation Examples 4-6

Using the Examples 1 to 3, Reference Examples 1 and 2 to prepare a scalp essence according to the composition of Table 9. More specifically, the efficacy materials of Examples 1 to 3 and Reference Examples 1 and 2, Polyoxyethylene Hydrogenated Castor Oils / POE (60) (Emalex HC-60, Nihon Emulsion Co., Ltd.). ), Salicylic acid 0.2%, ethanol 10%, distilled water, ammonium acryloyl dimethyl taurate / V-Picopolymer (manufacturer: Aristoflex) and phenoxyethanol (PE) were used.

(Unit: weight%) ingredient Formulation example Comparative formulation example 4 5 6 4 5 6 Polyoxyethylene Hydrogenated Castor Oil / POE (60) 0.5 0.5 0.5 0.5 0.5 0.5 Example 1 0.2 - - - - - Example 2 - 0.2 - - - - Example 3 - - 0.2 - - - Reference Example 1 - - - 0.2 - - Reference Example 2 - - - - 0.2 - Salicylic acid 0.2 0.2 0.2 0.2 0.2 0.2 ethanol 10 10 10 10 10 10 Distilled water 88.3 88.3 88.3 88.3 88.3 88.3 Ammonium Acryloyl Dimethyl Taurate / V-Picopolymer 0.5 0.5 0.5 0.5 0.5 0.5 Phenoxyethanol 0.3 0.3 0.3 0.3 0.3 0.3

Test Example 7 Skin Irritation Test

In order to check the skin irritation during use of the composition according to the present invention, 60 men and women 25 to 45 years of age who feel relatively severe scalp itch were selected and divided into 6 groups of 10 people each. Formulation Examples 4-6, Comparative formulation The scalp essences of Examples 4-6 were used. Specifically, after the back skin was washed, the water was wiped off and allowed to stand for 15 minutes in a constant temperature / humidity chamber (temperature 24 ± 4 ° C., relative humidity 40-45%). The same amount of the scalp essence containing 0.2% of the efficacious substance was applied to the cotton wool sufficiently to fix the position of the back skin. 24 hours and 48 hours after attaching the test substance, the subject expressed the degree of stimulation from 0 to 4 points (0 = no stimulus, 1 = very weak, 2 = weak, 3 = medium, 4 = severe). (2 measurements in total), irritation such as stinging, burning, and the like were evaluated.

Table 10 shows the number of subjects who complained of stimulation according to the grade for each time period. In addition, the average value was calculated in order to derive the skin irritation as a mean score (Mean score), the results are shown in Table 10 below.

Test substance name Test concentration 24hr 48hr Response Judgment One 2 3 4 One 2 3 4 (n = 10) Formulation Example 4 0.20% One 0 0 0 0 0 0 0 One No irritation Formulation Example 5 0.20% One 0 0 0 0 0 0 0 One No irritation Formulation Example 6 0.20% One 0 0 0 0 0 0 0 One No irritation Comparative Formulation Example 4 0.20% One 0 0 0 0 0 0 0 One No irritation Comparative Formulation Example 5 0.20% One 0 0 0 0 0 0 0 One No irritation Comparative Formulation Example 6 0.20% 0 0 0 0 One 0 0 0 One No irritation

In the results of Table 10, the composition comprising the ginseng polysaccharide or green tea polysaccharide, or a mixture of these polysaccharides of Formulation Examples 4 to 6, Comparative Formulation Examples 4 and 5 has the same skin irritation as Comparative Formulation Example 6 that does not include a polysaccharide. None could be confirmed. Therefore, the composition according to the present invention can be seen that by using the ginseng and green tea-derived polysaccharide as an active ingredient, it is safe for the human body and has no skin irritation, and there is no side effect even when used for a long time.

Reference Example 3 Preparation of Formulation Examples 7-9 and Comparative Formulation Examples 5-9

Using the Examples 1 to 3, Reference Examples 1 and 2 to prepare a scalp and hair combined treatment composition according to the composition of Table 11 below. After preparing the oil phase and the water phase separately in the blender, slowly add the oil phase to the water phase, emulsify it at 3,000 rpm using a homomixer for 10 minutes, and then cool to room temperature. Combined treatments were prepared.

Ingredient (% by weight) Formulation example Comparative formulation example 7 8 9 7 8 9 Purified water balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) Example 1 0.5 - - - - - Example 2 - 0.5 - - - - Example 3 - - 0.5 - - - Reference Example 1 - - - 0.5 - - Reference Example 2 - - - - 0.5 - Cetyltrimethylammonium chloride 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Stearyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Preservatives, Colors and Flavors Quantity Quantity Quantity Quantity Quantity Quantity

Test Example 8 Sensory Test

For the scalp and hair combined treatment compositions of Formulation Examples 7-9 and Comparative Formulation Examples 7-9, a sensory test was conducted in the following manner. After dividing 60 professional evaluation personnel into 6 groups of 10 persons and using the scalp and hair treatments of Formulation Examples 7-9 and Comparative Formulation Examples 7-9 for each group, the evaluation items shown in Table 12 below The comparison test was performed, and the test was conducted with the lowest score as 1 and the highest score as 5. The test results are shown in Table 12 below.

Item Itchy scalp Scalp irritation Hair softness / smoothness Formulation Example 7 3.0 2.9 2.2 Formulation Example 8 3.0 3.0 2.4 Formulation Example 9 2.8 2.8 2.4 Comparative Formulation Example 7 3.3 3.1 2.1 Comparative Formulation Example 8 3.1 3.0 2.3 Comparative Formulation Example 9 4.1 3.8 1.5

Referring to Table 12, the formulation examples 7-9 and ginseng or green tea polysaccharides containing the comparative formulations 7-9 in comparison to the case of using the comparative formulation example 9 containing no polysaccharides in the scalp is reduced and irritation feeling It has also been alleviated, and it can also be seen that the hair condition is better. In particular, when using the formulation examples 7 to 9 containing a mixture of ginseng polysaccharide and green tea polysaccharide can be confirmed that the effect is particularly excellent.

[Example 1] Hair Tonic

Tonic was prepared in a conventional manner according to the composition described in Table 13.

ingredient Weight ratio (%) ethanol 50 menthol 0.02 glycerin 3 Salicylic acid 0.05 Ginseng Polysaccharide of Reference Example 1 0.5 Green Tea Polysaccharide of Reference Example 2 0.5 Spices and Colors A reasonable amount Purified water Balance (to 100)

[Example 2] Hair Lotion

To prepare a hair lotion in a conventional manner according to the composition described in Table 14.

ingredient Weight ratio (%) Cetostearyl alcohol 2.0 EDTA 2Na 0.2 Hydroxyethyl cellulose 0.5 Mineral oil 5.0 Ginseng Polysaccharide of Reference Example 1 0.5 Green Tea Polysaccharide of Reference Example 2 0.5 antiseptic A reasonable amount Spices and Colors A reasonable amount Purified water Balance (to 100)

[Example 3] Hair nourishing lotion

According to the composition described in Table 15 below, hair nutrient lotion was prepared by a conventional method.

ingredient Weight ratio (%) Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.7 Mineral oil 10.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Ginseng Polysaccharide of Reference Example 1 0.5 Green Tea Polysaccharide of Reference Example 2 0.5 antiseptic A reasonable amount Spices and Colors A reasonable amount Purified water Balance (to 100)

[Example 4] Hair Shampoo

To prepare a hair shampoo in a conventional manner according to the composition shown in Table 16.

ingredient weight% Purified water Balance (To 100) Ginseng Polysaccharide of Reference Example 1 0.5 Green Tea Polysaccharide of Reference Example 2 0.5 Soddylaurylsulfite 36.0 Cocamidopropyl Betaine 8.0 Palmidine Malate 2.0 Glycolstearate 1.5 Polyquaterny 윰 10 0.5 Sight Rick Acid 0.1 glycerin 2.0 Preservatives, Colors and Flavors Quantity

[Example 5] Hair Rinse

To prepare a hair rinse in a conventional manner according to the composition shown in Table 17.

ingredient weight% Purified water Balance (To 100) Ginseng Polysaccharide of Reference Example 1 0.5 Green Tea Polysaccharide of Reference Example 2 0.5 Propylene glycol 2.0 Cetyltrimethylammonium chloride 1.0 Cetyl alcohol 3.0 Stearyl alcohol 3.0 Mineral oil 0.5 Sight Rick Acid 0.2 Polydimethylsiloxane 1.0 Preservatives, Colors and Flavors Quantity

As described above in detail the specific parts of the present invention, it is apparent to those skilled in the art that such specific description is merely a preferred embodiment, thereby not limiting the scope of the present invention. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (16)

Contains ginseng polysaccharide and green tea polysaccharide as active ingredients,
The ginseng polysaccharide and green tea polysaccharide are water; At least one organic solvent selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform; Or by dipping the ginseng root extract or green tea leaf extract prepared by extracting for 3 to 24 hours at 38 ~ 42 ℃ using a mixed solvent of the organic solvent and water, and then separating the residue and the filtrate through filtration and centrifugation, A skin external preparation composition for improving at least one hair or scalp abnormal condition selected from the group consisting of dandruff, scalp itch, white hair, and hair loss, obtained by dropwise addition of ethanol to an isolated filtrate. According to claim 1, wherein the ginseng polysaccharide and green tea polysaccharide is an external composition for skin composition is contained in an amount of 0.001 to 10% by weight relative to the total weight of the composition, respectively. According to claim 1, wherein the ginseng polysaccharide and green tea polysaccharide is a skin external composition to be used in a ratio of 0.1 to 10: 1 by weight ratio. delete delete delete delete delete Contains ginseng polysaccharide and green tea polysaccharide as active ingredients,
The ginseng polysaccharide and green tea polysaccharide are water; At least one organic solvent selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform; Or by dipping the ginseng root extract or green tea leaf extract prepared by extracting for 3 to 24 hours at 38 ~ 42 ℃ using a mixed solvent of the organic solvent and water, and then separating the residue and the filtrate through filtration and centrifugation, Pharmaceutical composition for improving the condition of any one or more hair or scalp selected from the group consisting of dandruff, scalp itching, white hair, and hair loss, obtained by dropwise addition of ethanol to the separated filtrate. The pharmaceutical composition according to claim 9, wherein the pharmaceutical composition is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition containing the ginseng polysaccharide and the green tea polysaccharide as active ingredients. The pharmaceutical composition of claim 9, wherein the ginseng polysaccharide and the green tea polysaccharide are mixed and used in a ratio of 0.1 to 10: 1 by weight. delete delete delete delete delete