KR102495343B1 - Cosmetic Composition for promoting hair growth or preventing hair loss with the extraco of Schisandra Chinensis Seed - Google Patents

Abstract

The present invention relates to a cosmetic composition for promoting hair growth or preventing hair loss comprising Schisandra seed extract, and the cosmetic composition containing the Schisandra seed extract of the present invention is excellent in promoting hair growth or preventing hair loss.

Description

Cosmetic composition for promoting hair growth or preventing hair loss containing Schisandra chinensis seed extract {Cosmetic Composition for promoting hair growth or preventing hair loss with the extraco of Schisandra Chinensis Seed}

The present invention relates to a cosmetic composition for promoting hair growth or preventing hair loss, and more particularly, to a cosmetic composition for promoting hair growth or preventing hair loss comprising an extract of Schizandra chinensis seeds.

In general, the function of hair is to protect the human body from external stimuli, such as protecting the scalp and maintaining body temperature. These hairs are generated in hair follicles, and repeat the hair cycle divided into anagen stage, catagen stage, and telogen stage, and control the growth, maintenance, and loss of hair. .

There are about 100,000 hairs on the scalp, and the hair that normally grows falls out for some reason, resulting in a lack of hair or a decrease in the number of hairs. The hair cycle is transformed and shortened by internal and external factors such as hormones, stress, disease, environmental pollution, and smoking. do.

Hair follicles are skin appendages present in mammals and include the inner root sheath, outer root sheath, hair root, hair dermal papilla, hair matrix, and melanogenesis. cells (melanocytes) are present. Among them, dermal papilla cells are known to play an important role in the growth cycle of hair and androgenetic alopecia through cell proliferation as a type of fibroblasts in hair bulbs. In addition, it is known to regulate the cycle of hair growth by increasing the expression of IGF-1 and VEGF, growth factors of dermal papilla cells, and activating Wnt/β-catenin, which is reduced in the process of hair loss.

Currently, minoxidil and finasteride approved by the Food and Drug Administration (FDA) are representative drugs that promote hair growth. However, it has been reported that drug therapy requires long-term use and is accompanied by various side effects such as cardiovascular disorders, skin irritation, and dermatitis, and thus the development of natural products with safety and efficacy secured is urgently needed.

Korean Patent Registration No. 10-0841923 (2008.06.23) discloses a pharmaceutical composition for preventing hair loss and improving hair growth, which contains a mixed soluble extract of Schisandra nigra Max. in water and ethanol as an active ingredient. Korean Registered Patent No. 10-1981678 (2019.05.17), for promoting hair growth and preventing hair loss, containing mixed extracts of aloe, baeknyeoncho, Schisandra, aronia, cordyceps, zinnia mushroom and Penthorum chinense Pursh as active ingredients A cosmetic composition is described.

Regarding the composition for promoting hair growth and preventing hair loss using Schisandra chinensis extract, as described above, several patent technologies have already been known. However, a composition for promoting hair growth and preventing hair loss using an extract of Schizandra chinensis seeds has never been developed. Therefore, the present invention is to develop and provide a cosmetic composition for promoting hair growth or preventing hair loss using Schizandra seed extract.

The present invention provides a cosmetic composition for promoting hair growth or preventing hair loss, comprising a seed extract of Schisandra chinensis.

In the cosmetic composition for promoting hair growth or preventing hair loss of the present invention, the seeds of Schizandra chinensis may be obtained by adding purified water to Schizandra chinensis, and treating with cellulase and pectinase enzymes.

In the cosmetic composition for promoting hair growth or preventing hair loss of the present invention, the cosmetic composition may preferably have a formulation selected from among shampoo, rinse and hair tonic.

The cosmetic composition containing the seed extract of Schisandra chinensis of the present invention is excellent in promoting hair growth or preventing hair loss.

1 is a qualitative analysis result of gomisin N, gomisin A, and sujandrin in Omaji seed extract.
Figure 2 is the results of the dermal papilla cell proliferation evaluation test of Schizandra chinensis seed extract, gomisin N, gomisin A, and susandin. (In Figure 2, '*', which is a sign of significance, indicates the significance of the result values of each sample of Gomisin N, Gomisin A, Sujandrin, and Schisandra Seed Extract)
Figure 3 is the IGF-1 expression test results of the seed extract of Schizandra chinensis, gomisin N, gomisin A, and susandin. (In Figure 3, '*', which is a sign of significance, indicates the significance of the result values of each sample of Gomisin N, Gomisin A, Sujandrin, and Schizandra seed extract.)
Figure 4 is the VEGF expression test results of Schisandra seed extract, gomisin N, gomisin A, and susandin. (In Figure 4, '*', which is a sign of significance, indicates the significance of the result values of each sample of gomisin N, gomisin A, sujandrin, and Schizandra seed extract)
5 shows the results of β-catenin expression experiments of Schizandra seed extract, gomisin N, gomisin A, and susandin.

Schisandra Chinensis is a fruit of the Schisandra chinensis tree and has a dark red ball shape with a diameter of about 1 cm. Inside, there are red juice and 1 or 2 reddish brown seeds, and the outer surface of the seeds is glossy yellowish brown to dark reddish brown, and there are clear seams on the back. It got its name because it has a sour taste in the skin, a sweet taste in the pulp, a spicy and bitter taste in the seed, and a salty taste overall.

The main components contained in Schisandra chinensis are Schizandrin, Gomisin A, N, Schizandrol, Epigomisin, Pregomoisin, and Deoxyschizandrin. etc. Gomisin N contained in Schizandra chinensis has a molecular weight of 400.46 and its structural formula is C ₂₃ H ₂₈ O ₆ . Gomisin A has a molecular weight of 416.47 and the structural formula is C ₂₃ H ₂₈ O ₇ . Susuzandrin has a molecular weight of 432.52 and the structural formula is C ₂₄ H ₃₂ O ₇ .

Schisandra chinensis is known to have effects such as saengjin, ansin, suhan, jigal, earth, and haeju poison. It is reported that Schisandra chinensis contains lignan components, and thus exhibits pharmacological activities such as anti-aging and cancer suppression effects, and cognitive and liver function improvement effects.

The present invention provides a cosmetic composition for promoting hair growth or preventing hair loss, comprising the seed extract of Schisandra chinensis characterized as described above.

The Schisandra seed extract of the present invention is water, alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane and mixtures thereof It can be extracted from Schizandra seeds through any one extraction method selected from solvent extraction, supercritical extraction and ultrasonic extraction using an extraction solvent selected from the group consisting of. Preferably, it is good to extract using alcohol or water having 1 to 4 carbon atoms, and most preferably, it is good to extract using 95.0% (v / v) pure ethanol.

In the cosmetic composition for promoting hair growth or preventing hair loss of the present invention, the seeds of Schizandra chinensis may be obtained by adding purified water to Schizandra chinensis, and treating with cellulase and pectinase enzymes. Preferably, the enzyme is good to use viscozyme L (Viscozyme L).

As a result of the experiment of the present invention, it was confirmed that the Schizandra seed extract of the present invention contains Gomisin N, Gomisin A, and Schizandrin. However, even though the Schizandra chinensis seed extract of the present invention is an extract, the activity of dermal papilla cell proliferation was not significantly lower than that of single compounds, Gomisin N, Gomisin A, and Susjandrin. In addition, in terms of IGF-1 expression ability and VEGF expression ability, the Schizandra chinensis seed extract of the present invention was similar to gomicin N, gomisin A, and susandin. In addition, in terms of β-catenin expression ability, the Schizandra chinensis seed extract of the present invention appeared similar to sujandrin, slightly lower than gomicin A, and relatively lower than gomicin N. These results indicate that the Schizandra chinensis seed extract of the present invention, despite being an extract, is a material that exhibits equivalent hair growth promotion and hair loss prevention effects compared to single compounds, Gomisin N, Gomisin A, and Susjandrin. could evaluate In addition, considering that the separation and purification process takes a lot of time and money, the Schizandra chinensis seed extract of the present invention can be evaluated as a very economical material compared to gomisin N, gomisin A, and susandin.

On the other hand, in the cosmetic composition of the present invention, the Schisandra seed extract is preferably contained in an amount of 10 to 10,000 ppm based on the total weight of the cosmetic composition. If the content of the extract is less than 10 ppm, the dermal papilla cell proliferation effect is insignificant, and if it is 10,000 ppm or more, the degree of dermal papilla cell proliferation effect increase for the content increase is insignificant, and there is a problem with the safety and stability of the formulation, and is not economical. not.

On the other hand, the composition for hair of the present invention may be prepared in any formulation commonly prepared in the art, and may be formulated into, for example, shampoo, rinse, hair tonic, and the like. However, it is not necessarily limited thereto.

Hereinafter, the contents of the present invention will be described in more detail in the following examples. However, the scope of the present invention is not limited only to the following examples, and includes modifications of equivalent technical ideas.

[Example 1: Preparation of Schisandra Seed Extract]

(1) Separation of Schizandra seeds from Schizandra chinensis

After adding purified water to 10% (w/v) of Schisandra chinensis, the fruit of Schizandra chinensis, 5% (v/v) viscozyme L (Novozyme) enzyme solution was added and extracted at 50° C. for 12 hours. The extract was filtered through a 400 mesh filter cloth, sterilized and inactivated to obtain Schizandra chinensis seeds.

(2) Preparation of Schisandra Seed Extract and Separation of Gomisin N, Gomisin A, and Susuzandrin

The seeds of Schisandra chinensis obtained above were dried and pulverized, and 5,000 g of 95.0% ethanol was added to 500 g of the pulverized material, followed by extraction at 45° C. and stirring at a speed of 200 rpm for 6 hours. The process was repeated by replacing the extraction solvent twice. The extract was centrifuged at 8,000 rpm for 15 minutes to obtain the supernatant. Thereafter, 68.4 g of Schizandra chinensis seed extract was obtained by concentrating under reduced pressure at 55° C. in a distillation apparatus equipped with a cooling condenser. This was purified by preparative high-performance liquid chromatography to separate gomicin N, gomicin A, and suzandrin, which were used in the following experiments. High performance liquid chromatography was used to evaluate the quality of gomicin N, gomicin A, and sujandrin, and the results were confirmed from FIG. 1.

[Experimental Example 1: Evaluation of dermal papilla cell proliferation]

In this experimental example, the cell proliferation effect on dermal papilla cells was measured for Schizandra chinensis seed extract prepared in Example 1, Gomisin N, Gomisin A, and Susjandrin.

Human-derived dermal papilla cells (human follicle dermal papilla cells, HFDPC) were dispensed into a 96 well plate at 1 × 10 ⁴ cells/ml, and stabilized for 24 hours in a 37°C, 5% CO ₂ cell incubator. After exchanging it with DMEM medium containing 1% FBS, the samples were diluted according to concentration and reacted for 48 hours. Then, 20 μl of MTT reagent at a concentration of 5 mg/ml was added to each well and reacted for 2 to 3 hours in a 37° C., 5% CO ₂ cell incubator. After the reaction was completed, the supernatant of each well was removed, and 100 μl of DMSO was added to completely dissolve the cells, and the absorbance was measured at 550 nm. At this time, the positive control group used minoxidil, a hair growth promoting agent, and the cell proliferation rate of each sample group was calculated by comparing the absorbance value of the control group.

Fig. 2 shows the results of the dermal papilla cell proliferation evaluation test for Schizandra chinensis seed extract, gomisin N, gomisin A, and sujandrin. As shown in FIG. 2, it was confirmed that all of gomicin N, gomicin A, suzandrin, and Schizandra chinensis seed extract exhibited dermal papilla cell proliferation activity. However, Gomisin N showed a higher proliferation rate of dermal papilla cells compared to other experimental groups.

[Experimental Example 2: Hair Growth Factor Expression Promotion Effect]

Dermal papilla cells were dispensed into 96 wells at a concentration of 1 × 10 ⁵ cells/ml and cultured for one day at 37°C, 5% CO ₂ incubator, and then the samples were treated by concentration and reacted in a 37°C, 5% CO ₂ incubator for 24 hours. . It was measured by enzyme-linked immunosorbent assay (ELISA) using the cell culture medium.

The expression of IGF-1 (Insuline-like growth factor) and VEGF (Vascular endothelial growth factor) in the cell culture was analyzed using an ELISA kit, and the absorbance was taken at 450 nm, and the respective production amounts are shown in FIGS. 3 and 4 . At this time, the positive control group used minoxidil, a hair growth promoting agent.

The experimental results were the same as in FIGS. 3 and 4. In terms of IGF-1 expression ability and VEGF expression ability, the Schizandra chinensis seed extract of the present invention was similar to gomisin N, gomisin A, and suzandrin.

[Experimental Example 3: Confirmation of Wnt/β-catenin expression rate]

Dermal papilla cells were dispensed into 6 wells at a concentration of 2 × 10 ⁵ cells/well and cultured in a 37°C, 5% CO ₂ incubator for one day, and then the samples were treated by concentration and cultured for 24 hours. The cultured cells were collected and lysed, and proteins were quantified using BCA assay. The quantified proteins were electrophoresed on a 10% SDS-PAGE gel and then transferred to a PVDF membrane. After shaking the primary antibody (β-catenin, santa cruz, USA) overnight at 4°C, the HRP-conjugated secondary antibody was reacted at room temperature for 2 hours. After that, it was washed three times with 1 × TBST and the band was confirmed using an ECL reaction kit. The intensity of each band was quantified based on the expression of β-actin to calculate the β-catenin expression rate. At this time, the positive control was shown in FIG. 5 using minoxidil, a hair growth promoter.

As shown in FIG. 5, in terms of β-catenin expression ability, the Schisandra seed extract of the present invention appeared similar to Susuzandrin and slightly lower than that of Gomisin A. And compared to gomishin N, it appeared relatively low.

[Formulation Example 1: Preparation of Shampoo Composition Containing Schizandra Seed Extract]

As a composition for preventing hair loss containing 5% by weight of Schizandra seed extract obtained in Example 1, Formulation Example 1 prepared as a shampoo formulation is shown in Table 1 below. At this time, a shampoo formulation without the Schisandra seed extract (Example 1) of the present invention was prepared in Comparative Example 1.

ingredient Formulation Example 1 (% by weight) Comparative Example 1 (% by weight) Schizandra Seed Extract
(Example 1) 5.0 - Disodium EDT 0.03 0.03 Polyquaternium-10 0.1 0.1 Ammonium Lauryl Sulfate 40.5 40.5 Ammonium Laureth Sulfate 15.5 15.5 Cocamidopropyl Betaine 6.0 6.0 propylene glycol 2.0 2.0 sodium xylene sulfonate 2.0 2.0 Glycol Distearate 3.0 3.0 Cocamide FA 1.0 1.0 Lauramide FA 1.0 1.0 salicylic acid 0.13 0.13 Simethicone 3.0 3.0 antiseptic Appropriate amount Appropriate amount incense Appropriate amount Appropriate amount Purified water balance balance total 100.0 100.0

[Experimental Example 4: Formulation Example 1 Containing Schizandra Seed Extract, Confirmation of Hair Loss Prevention Effect of Composition for Preventing Hair Loss]

In Experimental Example 4, the hair loss prevention effect of the shampoo prepared in Formulation Example 1 was confirmed.

After randomly dividing 20 adult males who recognized that they had hair loss into 2 groups (A and B) of 10 people each, the experiment was conducted for 4 weeks with 10 people in the experimental group (A) and 10 people in the control group (B). The composition of Formulation Example 1 for group A and the composition of Comparative Example 1 for group B were used at intervals of 2 days, respectively, and the results were observed. The degree of hair loss prevention was evaluated by the test subjects after the experiment was completed (-3: very worse, -2: worse, -1: slightly worse, 0: no change, 1: slightly improved, 2: improved, 3: very improved) According to the sensory evaluation, it is shown in Table 2 below.

subject Degree of hair loss prevention Group A
(formulation example 1) group B
(Comparative Example 1) One One 0 2 3 -3 3 One 0 4 2 -2 5 2 -One 6 0 -3 7 3 0 8 3 -One 9 2 0 10 One -2 average 1.8 -1.2

As shown in Table 2, the composition of Formulation Example 1 was found to have an excellent hair loss prevention effect compared to the composition of Comparative Example 1.

[Formulation Example 2: Rinsing Composition Containing Schizandra Seed Extract]

According to the contents of Table 3 below, a rinse composition containing Schizandra seed extract was prepared in Formulation Example 2.

ingredient Formulation Example 2
(weight%) Schisandra Seed Extract 5.0 Disodium EDT 0.02 Hydroxyethyl Cellulose 0.2 panthenol 0.05 propylene glycol 1.5 glycerin 2.0 cetyl alcohol 3.5 Stearyl Alcohol 1.5 Glyceryl Stearate SE 0.5 dimethicone 1.5 antiseptic Appropriate amount incense Appropriate amount Purified water balance total 100.0

[Formulation Example 3: Hair Tonic Composition Containing Schizandra Seed Extract]

According to the contents of Table 4 below, a hair tonic composition containing Schizandra seed extract was prepared in Formulation Example 3.

ingredient Formulation Example 3
(weight%) Schisandra Seed Extract 5.0 Disodium EDT 0.02 panthenol 0.5 Butylene Glycol 10.0 ethanol 18.0 menthol 0.03 Polysorbate 80 1.0 imidazolidinyl urea 0.2 biotin 0.1 incense Appropriate amount Purified water balance total 100.0

Claims (3)

It contains Schizandra seed extract,
The Schizandra seed extract,
A cosmetic composition for promoting hair growth or preventing hair loss, characterized in that the seeds of Schisandra chinensis obtained by adding purified water to Schisandra chinensis, and treating with cellulase and pectinase enzymes are extracted after drying and grinding.
delete According to claim 1,
The cosmetic composition,
A cosmetic composition characterized in that it has a formulation of any one selected from shampoo, rinse and hair tonic.

Images