KR101132246B1 - The hair growth solution mainly comprised of ginsenoside rg3 and rh2, and the hair treatment composition - Google Patents

Abstract

PURPOSE: A composition containing ginsenoside Rg3 and Rh2 is provided to prevent alopecia and promote hair growth. CONSTITUTION: A cosmetic composition for promoting hair growth contains 0.0001-20.0 wt% of ginsenoside Rg3 and Rh2 in a weight ratio of 2:1-8:1. A hair agent contains the cosmetic composition. The hair agent is manufactured in the form of a mixture among shampoo, hair treatment, hair tonic, hair pack, shampoo, and rinse.

Description

Hair growth promoting cosmetic composition mainly comprising ginsenoside Al g 3 and H 2 and hair cosmetics containing the same {The hair growth solution mainly comprised of ginsenoside Rg 3 and Rh 2, and the hair treatment composition}

The present invention relates to a composition for promoting hair growth and hair growth, and more particularly, to a hair growth agent containing a specific component of ginsenosides in an appropriate ratio to maximize its performance and to a hair cosmetic agent containing the same.

The composition according to the present invention contains ginsenosides Rg ₃ and Rh ₂ isolated from red ginseng and ginseng. As a result of the study of the present inventors, when the ginsenoside Rg ₃ of formula 1 and Rh ₂ of formula 2 were combined, the hair growth effect was achieved, and the hair growth and wool effect when included in the ratio of 2: 1 to 8: 1. Appeared to be the most outstanding.

The composition of the present invention and the cosmetics including the same have excellent hair loss prevention effect, hair growth and hair growth promoting effect, and do not cause side effects even when used for a long time, thus promoting hair growth and hair growth promoting hair applied to hair and scalp for a long time. It can be usefully applied as a cosmetic.

Human hair protects the scalp from skull protection, temperature control, and external shocks and ultraviolet rays. It is also an important factor in appearance and affects social activities, preventing hair loss and hair loss regardless of age or gender. Interest is constantly increasing.

Hair growth has its limitations, and after a certain period of time it stops growing and naturally falls off. According to the hair menstrual cycle, hair naturally falls within 100 per day and newborn hair grows, and the hair regeneration is repeated dozens of times in a lifetime.

However, as the number of hairs lost due to external stimulation or internal factors increases, thinning of hair decreases. This condition is called alopecia medically. In other words, the hair loss from the scalp is more than the new hair relatively hair thinning is an abnormal phenomenon, the nutrients and metabolism necessary for hair production can be seen as a kind of pathology caused by not naturally caused by any factor.

Hair loss is characterized by a short period of growth phase, a long period of rest period, and the newly grown hair is not completely grown and dropping out.

There are many causes of hair loss, including genetic factors, the effects of male hormones, and aging phenomena with age. However, the cause of hair loss can be thought to be caused by a combination of hair loss rather than by any one.

Specifically, enzyme inhibition and abnormalities involved in malnutrition, hair growth and growth, disorders of sebum secretion, hair root deformation, scalp drying and infection, scalp tension are known, and local infection, endocrine disorders, vascular disorders, stress, etc. Several factors are suggested. There is a recent study on the cause of immunology, but the direct evidence is unknown.

In modern society, hair loss is increasing due to internal and external factors such as pollution and stress. One of the most frequently mentioned is related to mental stress. As social stress increases, premature hair loss, which is more common in younger people, is on the rise, and about 80% of men over the age of 20 are suffering from alopecia. In addition, the number of younger patients treated with alopecia has recently increased, and in particular, the proportion of female patients has increased. This seems to be due to the social atmosphere and economic power, which are increasing interest in the external sector.

As the number of patients with alopecia and social interest increase, and the demand for treatment of hair loss and related drugs and foods increases, much attention is focused on methods for preventing and preventing hair loss, and various management forms for hair loss management are also developed. It is becoming.

The most commonly used medical method is hair transplant surgery, and the chemical method for enhancing the hair growth effect is minoxidil (minoxidil ^ⓡ ), which is currently recognized as a hair regrowth topical agent by the US Food and Drug Administration. In addition, propecia (propecia ^ⓡ ) and the like are used a lot.

However, hair transplantation has a high economic burden and difficulty in managing after the procedure, and this is fundamentally not a method of treating hair roots and hair follicles. In addition, hair growth agents added with chemicals to improve hair growth have the disadvantage that side effects and continuous effects other than short-term effects can not be achieved.

In particular, such as minoxidil was developed and approved as a treatment for hypertension, but developed and approved as a hair regrowth that is applied when hirsutism appears as a side effect in clinical trials. Minoxidil is a drug that shows the hair growth effect by increasing blood flow in the hair roots, and thus it is not available for hypotensive patients. Therefore, the dosage should be prescribed differently depending on the constitution, and when it does not remain on the skin when applied to the scalp At the same time the effect is reduced is absorbed into the bloodstream causing a side effect such as skin irritation.

In such a situation, the present inventors have mixed the ginsenosides Rh ₂ and Rg ₃ derived from red ginseng and ginseng, and further adjusted the ratio, thereby producing a hair cosmetic having excellent hair loss prevention effect and hair growth and hair growth effect. .

Panax ginseng CA Mayer is a representative medicinal plant that has been used for thousands of years and is a perennial herb belonging to the Araliaceae. It contains triterpenoid saponins, polyacetylenes, phenolic compounds, polysaccharides, and peptidoglycans. Many studies have been conducted around ginsenosides and scientifically proven various pharmacological actions such as immune enhancement, cancer cell proliferation and metastasis suppression, antistress, antioxidant, memory and learning ability improvement. In addition to traditional Chinese medicine, the demand for medicine and functional supplements is increasing in modern medicine.

More than 30 kinds of ginsenosides of ginseng and red ginseng have been reported. Ginsenosides are known to affect various physiology by acting individually or in combination, and have been reported to have effects on the central nervous system stimulation effect, hyperactivity, and vasodilation. It is known to have an effect on nerve suppression, protein synthesis, adrenal cortex hormone promotion, insulin-like action, anti-inflammatory action, detoxification and erythrocyte lysis. In addition to the different chemical structures present in other plants, it has been found that ginsenosides of ginseng are very mild, not toxic by overdose, and have little hemolytic action.

Examples of ginsenosides of ginseng or red ginseng have been developed in connection with hair growth and hair growth, including a hair growth agent containing ginseng extract as an active ingredient (Japanese Patent Laid-Open Publication No. 1985-038314), a composition for preventing hair loss and promoting hair growth (Korean Patent 0834108). ), Red ginseng irradiated with liposomes, licorice, biotin, cooper peptide, nano-liposomal particles containing cytokines and cosmetics for hair care containing them (Korean Patent No. 9753437), composition containing ginsenoside Ro (Korean Patent Publication No. 2008- 084117), and many other studies and patents have evaluated the skin stability and formulation stability including its effects.

However, the above examples contained ginseng and red ginseng extract or ginsenoside as some active ingredients, or used a general mixture such as irradiated saponin, not a specific ratio when using the mixture.

Ginsenoside, which is separated from red ginseng, has a very low yield and is expensive compared to other materials, so it is economically inefficient when used as a business material and has a disadvantage in that it is not useful. Low. Therefore, there is a case where a random mixture of multiple ingredients including some ginsenosides is claimed to prevent hair loss and promote hair growth, such as using extracts of red ginseng or ginseng. Furthermore, there was no case that revealed the effect of promoting hair growth and hair growth in consideration of the formulation between the components.

In short, the pharmacological effects of various single components of ginsenosides isolated from red ginseng and ginseng have been scientifically verified, and many hair cosmetic compositions and cosmetics containing them have been developed, but nonetheless have increased their effectiveness. There is not much research on the complex action of specific ginsenosides, and there is little development of a formulation using them. In other words, conventional hair cosmetics and cosmetics including ginsenosides were mostly cosmetics developed for a comprehensive purpose with a very wide range of functions such as hair growth, hair growth, wool, or skin. As a result, a complex extract of ginseng and red ginseng was included, and as a result, ginsenoside was mostly contained as an active ingredient. In addition, when using a single substance or a mixture of specific ginsenosides, a general mixture such as irradiated saponinin was used, not at a specific ratio.

The present invention has been made in the background as described above, the present invention overcomes the existing limitations by effectively combining the specific components of ginsenosides derived from red ginseng and ginseng, hair loss and hair growth and hair growth effects are very excellent And its purpose is to provide hair cosmetics.

The inventors have developed an excellent hair cosmetic agent effective for promoting hair growth and hair growth, and at the same time, combining various ginsenosides and adjusting the ratio to maximize the effect, the present invention was able to devise the present invention.

The present invention having the above object is characterized in that the ginsenosides Rg ₃ and Rh ₂ as a hair restorer as a main component.

At this time, the weight ratio of ginsenoside Rg ₃ and Rh ₂ is characterized in that 2: 1 to 8: 1.

The present invention is also characterized in that the hair cosmetic containing the hair growth agent.

And the hair cosmetic is characterized in that any one of a shampoo, hair treatment, hair tonic, hair pack, a mixture of shampoo and rinse.

In addition, the hair cosmetic agent is characterized in that ginsenoside Rg ₃ and ginsenoside Rh ₂ is contained 0.0001 to 20.0% by weight as a solid in the total cosmetic.

Ginsenoside Rg ₃ and Ginsenoside Rh ₂ isolated from ginseng or red ginseng, the active ingredient according to the present invention, have an excellent hair growth effect, especially when both components are contained in a ratio of 2: 1 to 8: 1. Excellent effect.

In addition, since the hair restorer of the present invention is a natural extract, there is no side effect on the scalp unlike other synthetic single raw materials. In fact, the results of evaluating the safety of the extract was found to have no effect on the living body was confirmed that it can be applied to the scalp prepared by applying cosmetics to the skin for a long time.

Subsequent to the present invention, in the development of an extraction process and a processing process in which the composition ratio of ginsenoside Rg ₃ and ginsenoside Rh ₂ satisfies the above-described range of hair growth and hair growth promoting effects, existing ginsenoside Rg is developed. _Since the separation process of ₃ and ginsenoside Rh ₂ can be omitted and used as an industrial material, the economic burden will be reduced.

Hereinafter, the present invention will be described in detail.

A hair growth agent comprising ginsenoside Rg ₃ and ginsenoside Rh ₂ in a weight ratio of 2: 1 to 8: 1 as an active ingredient according to the present invention, hair makeup exhibiting excellent hair growth and hair growth promoting effect It can be used as an agent composition.

The composition for promoting hair growth and hair growth of the present invention can be prepared in any formulation that can be applied to the scalp and hair conventionally, for example, shampoo, hair treatment, hair tonic, hair pack, mixed form of shampoo and rinse, etc. .

In the case of preparing the hair cosmetic in such a formulation, other ingredients are not particularly limited and may be freely selected in a conventional manner.

The amount of the composition of the present invention can be appropriately adjusted according to the individual differences or dosage forms, such as age, the degree of lesions, it is preferable to use within the range of about 1000 ~ 5000 mg per day. The components contained in the composition of the present invention are components that have been clinically applied for a long time and demonstrated human stability, and do not cause side effects even when used for a long time. The composition of the present invention can be usefully used as a hair cosmetic, medicine or quasi-drug for improving hair growth and hair growth promotion.

In addition, the present invention is described in more detail by the following Examples and Experimental Examples, but the present invention is not limited in any way by these.

[Red Ginseng Extract and Ginsenoside Rg ₃  And Ginsenoside Rh ₂ Manufacture of

(1) Preparation of Red Ginseng Extract

The dried red ginseng was washed with purified water, dried and broken into small pieces, and then added to 1 L of 80% ethanol, extracted at 65 ° C. for 1 hour and 2 times, and filtered under reduced pressure to obtain a red ginseng extract.

(2) Preparation of Ginsenoside Rg ₃

After ginseng or red ginseng was washed with purified water, dried and broken into small pieces, ginseng irradiated with ginseng irradiated according to a conventional method from the extract obtained by using a 10-fold dry weight of ethanol as a solvent. 10 g of the irradiated saponin was dissolved in 35 ml of 50% acetic acid and heated with stirring at 70 ° C. for 2 hours, and then the reaction mixture was cooled to room temperature. The reaction solution was diluted with 300 ml of distilled water and extracted three times with 150 ml of n-butanol. The combined extracts were washed twice with saturated sodium bicarbonate solution and the n-butanol layer was concentrated under reduced pressure. The reaction mixture was separated by silica gel column chromatography (chloroform: ethanol: water = 100: 30: 10, lower layer) to obtain ginsenoside Rg ₃ .

(3) Preparation of Ginsenoside Rh ₂

After ginseng or red ginseng was washed with purified water, dried and broken into small pieces, ginseng irradiated with ginseng irradiated according to a conventional method from the extract obtained by using a 10-fold dry weight of ethanol as a solvent. Ginsenosides Rh ₂ were isolated from the isolated probeponin using a prep 4000 (Waters Co., Ltd. USA, Delta prep 4000). The separated ginsenoside Rh ₂ was qualitatively analyzed by HPLC (Waters Co., Ltd. USA, Waters 2690 Seperations Module, Waters 2487 Dual λ Absorbance deterctor) to confirm that the purity is 96% or more.

Preparation Example 1 Ginsenoside Rg ₃  And ginsenosides Rh ₂ Mixture of

The mixture of ginsenoside Rg ₃ and ginsenoside Rh ₂ was mixed as shown in the following table (fig. 1).

[Fig. One]

Experimental Example 1 Cytotoxicity Test

CCD986sk, a human skin fibroblast, was cultured to 4-5 × 10 ⁴ cells / ml, and then 100 µl of the solution was inoculated into 96 well plates at 37 ° C. and 5% CO ₂ incubator for 24 hours. .

100 μl each of the prepared ginsenoside Rg ₃ and the mixture of ginsenosides Rh ₂ and Rg ₃ / Rh ₂ were added to the normal cells cultured by the above method in 37 ° C. and 5% CO ₂ incubator for 48 hours. Incubated. After the incubation was completed, the supernatant was removed, and 100 µl of 4 ° C and 10% (w / v) TCA (trochloroacetic acid) was added thereto, and the mixture was allowed to stand at 4 ° C for 1 hour and washed 5 times with distilled water to remove TCA and plate at room temperature. Completely dried. 100 μl of 0.4% (w / v) SRB solution dissolved in 1% (v / v) acetic acid was added to each well of the dried plate and stained for 30 minutes at room temperature. To remove, wash 4 ~ 5 times with 1% acetic acid. After completely dried at room temperature, 100 μl of 10 mM Tris buffer was added to dissolve the staining solution, and the absorbance was measured at 540 nm using a micropalte reader to measure toxicity to normal cells.

As a result, fig. As shown in Fig. _2, both ginsenoside Rg ₃ and ginsenoside Rh ₂ showed less than 28% cytotoxicity against dermal fibroblasts, and the mixture of ginsenoside Rg ₃ / Rh ₂ was also lower than 26-21%. Toxicity has been shown to be suitable for use as a cosmetic and cosmetic raw material.

Fig. 2. Chart showing cytotoxicity results of Rg ₃ / Rh ₂ mixture according to the present invention

Experimental Example 2 Confirmation of the proliferative effect of dermal papilla cells

To confirm the proliferative effect of ginsenoside Rg ₃ and ginsenoside Rh ₂ and Rg ₃ / Rh ₂ mixture obtained in Example 2 on dermal papilla cells [Messenger, AG, (1984) The culture of dermal papilla cells from human hair holliceles. Br . J. Dermatol . 110: 685-689.] Was applied.

The dermal papilla cells isolated from human hair follicles were penicillin (100 Units / mL), streptomycin (100 ㎍ / mL), 2 mM L-gultamine (Gibco Inc, NY, USA) and 20% inactivated fetal calf serum (FBS Incubated in 37%, 5% CO ₂ incubator using DMEM (Hyclone Inc, USA) medium containing Gibco, Inc, NY, USA.

To determine the proliferative effect of dermal papilla cells, MTT assay [Mossman, BT (1983) In vitro approaches for determining mechanisms of toxicity and carcinogenicity by asbestos in the gastrointestinal and respiratory tracts. Environ Health Perspect. 53: 155-161.]. 1.0 × 10 ⁴ cells / ml were dispensed into 96 well plates and incubated in 37 ° C., 5% CO ₂ incubator for 24 hours. Experimental group was prepared ginsenoside Rg ₃ And the mixture of ginsenoside Rh ₂ and Rg ₃ / Rh ₂ , 100 g of red ginseng extract was treated, and the control group was added only the basic medium without the mixture of Rg ₃ / Rh ₂ and red ginseng extract. After incubation for 2 days, 50 mg of 2 mg / ml MTT (Sigma, MO, USA) solution was added to each well and reacted for 4 hours. The supernatant was removed, 200 μl of DMSO was added, followed by stirring for 10 minutes to uniformly dissolve formazan. The absorbance was then measured at 570 nm using a microplate reader.

Experimental results, Fig. As shown in 3, the Rg ₃ / Rh ₂ mixture of the present invention showed high cell proliferation compared to the control, red ginseng extract, single Rg ₃ and Rh ₂ . When red ginseng extract was treated with dermal papilla cells (105.0 ± 4.1%), it showed 0.9-fold growth for the proliferative effect of the control group (100%) and showed low growth compared to all Rg ₃ / Rh ₂ mixtures. In addition, Jin showed a lower growth than the ginsenoside Rg ₃ and ginsenoside Rh ₂ Fig. Rg ₃ / Rh ₂ mixture, Rg ₃ / Rh ₂ mixture 2: 1 to 8: the sample group contained in an amount of 1 152.5 ± 2% ~ 175.6 ± 0.2% showed the highest proliferation effect among the sample group treated with the cells.

In conclusion, the Rg ₃ / Rh ₂ mixture stimulates the growth of dermal papilla cells, which play an important role in hair growth, and induces hair growth by stimulating the matrix cells. In addition, the Rg ₃ / Rh ₂ mixture may be usefully used as a cosmetic composition having hair growth and hair growth promoting activity than a single Rg ₃ , Rh ₂ and red ginseng extract.

Fig. 3. Graph comparing the proliferative effect of dermal papilla cells of Rg ₃ / Rh ₂ mixture according to the present invention

Experimental Example 3 Acute Toxicity Measurement

Five-week-old male ICR mice were purchased from Oriental Bio, followed by a one-week acclimatization period. Each ginsenoside Rg ₃ and ginsenoside Rh ₂ prepared in the preparation of the present invention And the mixture thereof was intraperitoneally administered at a dose of 20 mg / kg, 50 mg / kg, and 100 mh / kg, respectively, and observed for 24 hours of toxicity.

As a result of the experiment, there were no individuals who died in all of the experimental group, and the stability of the preparation of the present invention was confirmed as no abnormal symptoms were found in comparison with the control group in weight gain and feed intake.

Preparation Example 2 Preparation of Shampoo for Promoting Hair Growth and Hair Growth

Fig. By mixing the raw materials as 4 to prepare a shampoo composition for promoting hair growth and hair growth.

Fig. 4.

Experimental Example 4 Hair Growth Effect Measurement

Five-week-old male C57BL / 6 mice were purchased from Oriental Bio, followed by a one-week purifying period. The number of animals per experimental group was set to 15.

To examine the hair growth effect, we used 6 week old mice with resting hairs with pink dorsal skin color. Hair removal was carefully performed using an animal clipper, and hair was completely removed using a hair removal agent. A predetermined amount of sample was sufficiently applied to the depilated area using a brush. Sample treatment was conducted once daily, and the wool effect was confirmed after 4, 7, 10 and 14 days from the start of the visual evaluation study of the hair growth. The wool effect was visually evaluated according to the following criteria (fig. 5) by visually assessing the degree of hair grown on the sample application site (the ratio of the area of hair growth after sample treatment out of the back hair removal area). The hair growth efficacy of this product was evaluated through a comparison test with other existing products.

Fig. 5. Criteria for Hair Growth Effect

Fig. 1 shows the experimental results for measuring hair growth and hair growth promoting effects. As shown in Fig. 6, the rapid growth of hair growth after 10 days was observed when the effect of the composition containing ginsenoside Rg ₃ and ginsenoside Rh ₂ in a ratio of 2: 1 to 8: 1 on C57BL / 6 mice. It has an effect, and showed faster hair growth with existing hair regrowth.

From the above results, it can be seen that the composition for promoting hair growth and hair growth according to the present invention is superior to the composition containing minoxidil as an active ingredient, and the active ingredient of the present invention is effective. All of them are derived from natural materials, so there are no side effects on long-term use, so there is an advantage that they are more excellent for application to cosmetics than compositions containing chemicals such as minoxidil.

Fig. 6. Hair growth and wool activity

From the results of the above, the combination of ginsenosides Rg ₃ and Rh ₂ in accordance with the present invention can achieve excellent effects in hair growth and hair growth, and in particular when the ratio of both is 2: 1 to 8: 1 by weight ratio It can be confirmed that it has efficacy.

Claims (5)

delete Ginsenoside Rg ₃ and Rh ₂ is a cosmetic composition for promoting hair growth, characterized in that contained in a weight ratio of 2: 1 to 8: 1. The cosmetic composition of Claim 2 containing the cosmetic composition. The method of claim 3,
Hair cosmetics are any of shampoos, hair treatments, hair tonics, hair packs, shampoos and rinses. The method of claim 3,
A hair cosmetic agent comprising ginsenoside Rg ₃ and ginsenoside Rh ₂ in an amount of 0.0001 to 20.0% by weight as a solid in the total cosmetic.