KR101343857B1 - A composition comprising ginsenoside f2 for promoting hair growth or inhibiting hair loss - Google Patents

Abstract

The present invention relates to an external use composition for promoting hair growth or inhibiting hair loss which includes ginsenoside F2 derived from fermented red ginseng as an active ingredient. The composition according to the present invention has enhanced stability and no side effects on skin and excellent effects of hair loss inhibition and hair growth by the fact that ginsenoside F2 helps follicular cell activation, thereby promoting hair growth and delaying hair loss.

Description

A composition comprising ginsenoside F2 for promoting hair growth or inhibiting hair loss, using ginsenoside F2 derived from fermented red ginseng as an active ingredient.

The present invention relates to an external preparation composition for promoting hair growth or preventing hair loss using ginsenoside F ₂ derived from fermented red ginseng as an active ingredient.

Hair loss refers to a condition in which there is no hair in the area where hair normally should be present, and generally means that the terminal hair of the scalp is missing. Modern medicine does not consider the cause of hair loss to be caused by heredity or aging, but is looking for various causes. Excessive stress, poor blood circulation in the scalp, androgen secretion, seborrheic dermatitis, genetic factors, malnutrition, iron deficiency, drugs, It is thought to be caused by a decrease in thyroid function. In addition, researches on a number of drugs and treatments for treating hair loss due to these causes are being actively conducted.

Current drugs that promote hair growth are approved by the US Food and Drug Administration (FDA), including Minoxidil, Finasteride, and Propecia. Minoxidil from Pharmacia & Upjohn Co. was initially developed as a vasodilator for the treatment of hypertension, but it was developed as a hair restorer when hirsutism was reported as a side effect. Minoxidil, which is widely used in pharmacotherapy, has not been clarified so far, but the mechanism of action on the hair growth effect of minoxidil is not clear yet, but it is thought that nutrient expansion and potassium channel opening effect through vasodilation induce hair growth. (Meisheri et al., 1988; Buhletal., 1990a, 1990b). Minoxidil is mainly applied to androgenetic alopecia, and its effect is confirmed for head alopecia, but it is ineffective for temporal alopecia, and it is also possible to cause side effects such as deprivation of antihypertensive action, hypotension, tachycardia, and cardiovascular complications. It is not suitable for long term use. Propecia, which inhibits the production, binding, and metabolism of androgen, a male hormone, can cause feminization of men, and women of childbearing age have a high risk of birth defects.

As such, drug therapy using chemicals causes an unbalance of homeostasis in the body, causing unexpected side effects, and there is a need for a drug that has no side effects, is excellent in preventing hair loss, and has an effect of promoting the proliferation of hair follicle cells. Has been constantly.

KR 10-2004-0105048 A, December 14, 2004

Therefore, the inventors of the present invention, while we are studying to develop a material that can promote hair growth, ginsenoside F ₂ of Formula 1 increases the number and size of hair follicles, more specifically hair follicle cells Has a function of promoting the growth of hair growth experiments using a mouse as a result of the significant hair growth promoting function was confirmed, the present invention was completed.

An object of the present invention is to provide an external composition for promoting hair growth or preventing hair loss containing ginsenoside F ₂ as an active ingredient having excellent hair growth promoting effect and which can be safely used without side effects on the skin.

In order to achieve the above object, the present invention provides a composition for promoting hair growth or preventing hair loss containing ginsenoside F ₂ of the general formula (1) as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention 20 (S) extracted from the fermented ginseng-protocol wave incident diol-3-0-beta-D-glucoside-pyrazol-shi Figure -20- 0-beta-D-gluconic nose Llano side (20 (S) -protopanaxadiol -3- O - β- D-glucopyranosido-20- O-β- D-glucopyranoside (hereinafter abbreviated as "ginsenoside F ₂ ") as an active ingredient.

Ginsenoside F ₂ of the formula (1) as an active ingredient in the composition of the present invention is not contained in the root of Korean ginseng species, 0.2% in Korean ginseng leaves, 0.018% in American ginseng root, 0.1% in flower buds of Chinese ginseng, It contains 0.02% in the head of maliya ginseng, and it has been found to have various excellent effects as a metabolite of intestinal bacteria of ginseng saponin.

In addition, ginsenoside F ₂ has been shown to have various effective effects such as improving atopic dermatitis, anti-inflammatory and anti-inflammatory effects, inhibiting tumor cell proliferation, and reversing multidrug resistance in tumor cells or bacteria through efficacy experiments. It is not contained in the roots, and when ingested ginseng saponin is low in the intestinal metabolite, the yield is low, it is difficult to obtain a large amount of samples at the time, the study of pharmacological efficacy is very few.

Thus, the inventors of the present invention while studying to develop a substance capable of promoting hair growth, ginsenoside F ₂ of the formula (1) hair follicle cell (hair follicle cell) activation, more specifically, hair follicle cells to promote the growth of hair follicles Increasing the number and size, and confirmed that the hair growth experiment using the mouse has a significant hair growth promoting function, the present invention was completed.

In the present invention, ginsenoside F ₂ of the formula (1) can be used ginsenoside F ₂ derived from the red ginseng, preferably extracted and separated from fermented red ginseng.

There is no limitation on the type of red ginseng, any red ginseng available in the art can be used.

In the fermented red ginseng, the fermentation treatment of red ginseng can be carried out according to various fermentation methods known in the art.

The fermentation treatment may be performed by (i) directly inoculating fermentation microorganisms into an extract, (ii) by using a medium (eg, crude enzyme solution) in which the fermentation microorganisms are cultured, or (i) or by changing the structure of the desired polysaccharide. This can be done directly using the enzymes that can be induced.

No microorganism used in fermentation is not particularly limited, preferably the Aspergillus (Aspergillus) and the microorganism of the genus Lactobacillus (lactic acid bacteria ).

When the Aspergillus (Aspergillus) in the microorganism to be used, preferably from Aspergillus awamori (Aspergillus awamori ), Aspergillus ficuum ), Aspergillus niger), Aspergillus come dissimilar (Aspergillus oryzae ) and Aspergillus microorganisms selected from the group consisting of sojae ) are used, more preferably, Aspergillus niger, Aspergillus olai and Aspergillus, most preferably Aspergillus.

Lactic acid bacteria suitable for the present invention include various lactic acid bacteria known in the art. As used herein, the term "lactic acid bacteria" refers to a group of bacteria that produce lactic acid as the main product of carbohydrate metabolism. Preferably, the lactic acid bacteria used in the present invention, Lactococcus , Lactobacillus , Lactobacillus , Leuconost oc, Propionibacterium , Enterococcus , Bifidobacterium ( Bifidobacterium ), Streptococcus and Pediococcus are lactic acid bacteria selected from the group consisting of. More preferably, the lactic acid bacteria used in the present invention is Lactobacillus alimentarius ( Lactobacillus) alimentarius , Lactobacillus sakei ), Lactobacillus acidophilus ), Lactobacillus casei , Lactobacillus gasseri), Lactobacillus del Brewer ekki (Lactobacillus delbrueckii), Lactobacillus momentum spread (Lactobacillus fermentum , Lactobacillus bulgaricus), Lactobacillus helveticus (Lactobacillus helveticus , Leuconostoc, mensenteroides ), Streptococcus thermophilus , Streptococcus lactis ), enterococcus faecium ( Enterococcus faecium ), Enterococcus faecalis), Bifidobacterium William BP bonus (Bifidobacterium bifidum), Bifidobacterium William Infante Tees (Bifidobacterium infantis), Bifidobacterium William Bra Entebbe (Bifidobacterium brave , and Bifidobacterium longum .

The extraction method is a known conventional extraction solvent, preferably, (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (e.g. methanol, ethanol, propanol, butanol, normal-propanol, iso- Propanol and normal-butanol, etc.), (c) the mixed solvent of the lower alcohol and water, (d) acetone, (e) ethyl acetate, (f) chloroform, (g) 1,3-butylene glycol, (h ) Hexane, (i) diethyl ether, or (j) butyl acetate, more preferably (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (e.g. methanol, Ethanol, propanol, butanol, normal-propanol, iso-propanol and normal-butanol, etc.), and (c) a mixed solvent of the lower alcohol with water, most preferably water, methanol or ethanol.

Ginsenoside F ₂ of the formula (1) of the present invention is not only obtained by using the above-mentioned solvent, the fermentation extract, but also obtained by additionally applying a purification process. For example, fractions obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity), etc. The fraction obtained through the purification method is also included in the red ginseng fermentation extract of the present invention.

In the present invention, the content of ginsenoside F ₂ of Formula 1 may include 0.001 to 50% by weight, preferably 0.001 to 10% by weight, more preferably 0.01 to 5% by weight based on the total weight of the composition. . If the ginsenoside F ₂ content of Formula 1 is less than 0.001% by weight, the hair loss prevention and hair regrowth effect is too weak, and if the content exceeds 50% by weight, the hair loss prevention and hair growth increase due to the content increase are insufficient. There is a problem that the stability of the shape is not secured.

The composition of the present invention can be used in a variety of hair cosmetics having a hair loss prevention and hair growth promoting effect. Examples of the product to which the composition can be added include, for example, balmonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisturizer. Cream, hair massage cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair colorant, hair wave agent, hair bleach, hair gel, hair glaze, Cosmetics such as hair dressers, hair lacquers, hair moisturizers, hair mousses or hair sprays and the like.

Hair cosmetics of the present invention may further comprise a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included.

The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

The oil-soluble vitamin may be any compound that can be formulated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantotenylethyl Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

In addition to the said essential component, you may mix | blend the other cosmetics normally mix | blended with cosmetics to the hair cosmetic of this invention as needed.

Examples of the blending component that may be added include oil or fat components, humectants, emollients, surfactants, organic and inorganic pigments,

Organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil components include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethylhexanopenta lysitol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoate cetostearyl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin Fatty Acid Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POE / POP (Polyoxyethylene / Polyoxypropylene) Copolymer, POE / POP Alkyl Ether, Polyether Modified Silicone, Laurin Acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- , 3,5-triazine, 2- (2-hydroxy Methyl-5-methylphenyl) benzotriazole, and the like.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Although any said component can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.001-5 weight% with respect to the total weight of a composition, More Preferably it is compounded at 0.01 to 3 weight%.

Components included in the composition of the present invention may include components commonly used in external preparation compositions other than the ginsenoside F ₂ as an active ingredient, for example, conventional stabilizers, solubilizers, vitamins, pigments and flavorings. Phosphorus aids and carriers.

The composition of the present invention may be prepared in any formulation conventionally prepared in the art, and includes, for example, emulsions, creams, lotions, packs, cosmetics, and the like which can be used for hair.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The composition according to the present invention is excellent in stability to the skin by using ginsenoside F ₂ as an active ingredient, there is no side effect on the skin, and ginsenoside F ₂ is Due to the hair follicle cell activation ability, it promotes hair growth and delays hair loss, and has an advantage of preventing hair loss and hair growth.

1 is a result of Example 1 of the present invention, the naked eye effect in mice after 2 weeks of application of 3% minoxidil (MXD) drug and 0.5% ginsenoside F ₂ (Ginsenoside F ₂ , GinF ₂ ) As observed
FIG. 2 shows the effect of hair growth in mice after 4 weeks of application of 3% minoxidil (MXD) drug and 0.5% ginsenoside F ₂ (Ginsenoside F ₂ , GinF ₂ ) as a result of Example 1 of the present invention. As observed.
FIG. 3 shows the results of Example 1 of the present invention over time after application of 3% minoxidil (MXD) drug and 0.5% ginsenoside F ₂ (Ginsenoside F ₂ , GinF ₂ ) (2 weeks and 4 weeks) is the result of measuring the hair growth index,
Figure 4 is the result of a second embodiment of the present invention, 3% minoxidil (Minoxidil, MXD) drug and 0.5% ginsenosides F ₂ (Ginsenoside F _2, GinF ₂₎ applying a result of measuring the anagen hair growth after 4 weeks, and ,
5 is a result of Example 3 of the present invention, the growth of the hair growth period of the entire hair growth site after the application of 3% minoxidil (MXD) drug and 0.5% ginsenoside F ₂ (Ginsenoside F ₂ , GinF ₂ ) The ratio is the result of image analysis over time (1 week, 2 weeks, 4 weeks),
FIG. 6 shows that as a result of Example 3 of the present invention, grown hairs were grown in the entire hair loss site after 4 weeks of application of 3% minoxidil (MXD) drug and 0.5% ginsenoside F ₂ (Ginsenoside F ₂ , GinF ₂ ). Is the result of visual observation of the proportion of the site,
7 is a result of confirming the development of hair roots after 4 weeks of untreated C57bl / 6 mice and 1% TXN-coated mice as a result of Example 4 of the present invention.
Figure 8 is the development of a result of a fourth embodiment of the present invention, 3% minoxidil (Minoxidil, MXD) drug and 0.5% true after four weeks of ginsenoside _{F 2 (Ginsenoside F 2, GinF} 2) coating a mouse hair follicles The result is confirmed by the organization picture.

The present invention will be described in more detail with reference to the following examples. However, the following examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited by these examples in any sense.

Hereinafter, the technical and scientific terms used herein will be understood by those skilled in the art without departing from the scope of the present invention. Descriptions of known functions and configurations that may be unnecessarily blurred are omitted.

Example 1 Ginsenoside F ₂ Production of

15-20 times of purified water was added to red ginseng, and the pH was adjusted to 5.5. Aspergillus niger strain was inoculated 1% by weight, fermented at 37 ° C. for 96 hours, and then lyophilized to be fermented red ginseng. Got water.

The changes before and after the fermentation of ginseng saponin components of the red ginseng fermentation were confirmed using high performance liquid chromatography (HPLC, Waters, Inc. USA).

As the detector, an evaporative light scattering detector (ELSD) and an ultraviolet (UV) detector (203 nm) were used, and 0.05% trifluoric acetic acid was added to acetonitrile in water and acetonitrile as HPLC solvents. Used.

Each material was separated and detected using a concentration gradient of water and acetonitrile using a C18 reversed phase column of 4.6 × 250 mm. The standard material of ginseng saponin was self-separated. As a result, it was confirmed that the ginsenoside F ₂ was increased by about four times.

Experimental Example 1 Ginsenoside F ₂ Investigation of Efficacy in Wool Promotion

In order to confirm the hair growth effect of ginsenoside F ₂ obtained in Example 1, the area of wool in the animal model was evaluated.

6-week-old C57BL mouse (25 g) was used as an electric clipper (ER143 / Panasonic / Japan), and the length from the dorsal neck to the base of the tail was 1 cm from the left and right sides of the dorsal midline. The above width was depilated.

1% testosterone (Testosterone) dissolved in ethanol to uniformly suppress the hair growth of mice after grouping according to the Z-array method on the basis of weight by screening subjects with homogeneous pink color and no abrasion and severe redness. , TXN) was soaked in cotton and applied to the hair removal site for 1 week.

After percutaneous a 1% TXN (negative control) in the hair removing part coating, a 3% minoxidil (Minoxidil, MXD), the drug, the test group 0.5% ginsenosides _{F 2 (Ginsenoside F 2, GinF} 2) 200 ㎕ as a positive control After dispensing at the tip of the brush using a micropipette (micropipette) and applied homogeneously once a day for 4 weeks in the entire hair removal site in the vertical direction.

In order to evaluate the area of wool, 5 days of hair removal were designated using a digital camera (Canon EOS 30D / Canon / Japan) on 0, 1, 2, 3 and 4 weeks. The pictures were taken at a photo stand (Copy stand CS-30 / LPL / Japan) under 200 to 300 lux room light in the place.

The conditions for each photographing are the same as the distance of 70 cm from the subject, and the lens specifications (canon zoom lens, EF 28-105 mm 1: 3.5 -4.5 II) and the mode conditions are automatic mode (AI focus on, Photographs were taken with digital images of 1504 × 1000 with the central subject focus, auto white balance, and flash. However, for experimental purposes, photographing on the 0th was taken immediately before the application of the first test substance, and subsequent photographing was taken before the application of the test substance on the day. Animals used in photography were taken by light anesthesia using dimethyl ether and photographed, and the hair growth index was measured and quantified.

(1) Visual observation of hair growth

As a result of analyzing the photographs for visual observation of the hair growth effects for 2 weeks and 4 weeks, as shown in FIGS. 1 and 2, the mice in the test group coated with 0.5% ginsenoside F ₂ had no skin reactions. Hair grows and grows constantly Ginsenoside F ₂ It was confirmed that there is a significant hair growth promoting effect. On the other hand, the mice treated with 3% MXD used as a positive control were found to have severe inflammatory reactions on the skin.

(2) Hair Growth Index Survey

Based on the contents of the following Table 1 from the experimental animal was measured and quantified by the hair growth index.

As a result of examining the hair growth index for 2 weeks and 4 weeks, as shown in the results of FIG. 3, the hair growth growth index of 4 weeks was 3.8 for the C57bl / 6 normal group and 1.6 for the 1% TXN negative control group. On the other hand, in the positive control group coated with 3% MXD drug, the hair growth index was increased to 3.2, and the hair growth index increased to 50.0% or more compared to the 1% TXN negative control group.

In contrast, the test group coated with 0.5% ginsenoside F ₂ had a hair growth index of 3.5, which was about 0.3 higher than that of the positive control group coated with 3% MXD.

In other words, the above results indicate that the hair growth index increased by about 0.3 than that of the positive control group to which the drug was applied, but the hair growth index was higher than 3.0 even at 0.5% concentration, which is 6 times lower than the MXD of the positive control group. , It is a result confirming that the hair growth effect is superior to the drug of the positive control.

Example 2 Growth Hair Growth Measurement

Hair growth was inhibited by applying 1% TXN for 1 week to depilated C57bl / 6 mice, and applying 0.2% of 0.5% ginsenoside F ₂ for 4 weeks per day for 0.1 weeks using a vernier caliper. up to mm was observed.

4 weeks growth hair growth value of two hairs from each back of the mouse measured by Bernia caliper, as shown in Figure 4, 1% TXN negative control compared to the normal group of C57bl / 6 mice more than double the hair It was confirmed that the growth of.

However, the positive control group coated with 3% MDX (p <0.01) and the test group coated with 0.5% ginsenoside F ₂ (p <0.05) showed more than 45% increase in hair growth compared to the negative control group. As a result, it was confirmed that the hair growth promoting effect on growth hair is excellent.

Example 3 Image Analysis Software for Hair

In order to visually check the hair growth state at 1 week (7 days), 2 weeks (14 days), and 4 weeks (28 days) of the experiment of Example 1, after taking anesthesia with ether (Nikon, D70, Tokyo, Japan), and after visually comparing the degree of hair growth, the image was analyzed by image analysis software (Each system ships with 1 full version of Quantity One and unlimited copies of Quantity One Basic Mode.Bio-Rad, USA). The ratio of the area in which hair growth was grown among the entire hair loss site was determined (ratio of black changed area of the entire hair site).

As a result of confirming the proportion of the hair growth stage of the entire hair loss site of the dorsal part of the final 4 weeks of application, as shown in Table 2, FIG. 5 and FIG. In the experimental group coated with 3% MDX and the experimental group coated with 0.5% ginsenoside F _2, 213.46 and 216.96, respectively, were compared with the negative control group. It was confirmed that the ratio of the site was increased (p <0.01), and it was confirmed that the promoting effect of hair growth of the growing hair was excellent. , As can be seen in Table 2, FIG. 5 and FIG. 6, the C57bl / 6 normal group was 247.9, and the 1% TXN negative control group was 159.29, compared with 3% MDX. 213.46 and 216.96, respectively, in the positive control group and the 0.5% ginsenoside F ₂ -coated test group, which were statistically significant compared to the negative control group (p <0.01) to increase the ratio of growing hair to the hair growth site It was confirmed that it was confirmed that the hair growth hair growth promoting effect is excellent.

[ Experimental Example  4] Histological Analysis

At 4 weeks after the administration of the MXD drug and ginsenoside F ₂ of Experimental Example 1, the application site was extracted and fixed using 10% formalin buffer.

After embedding in paraffin through a conventional tissue treatment process, 4 μm paraffin tissue sections prepared using a microtome (HM-315 model, Microm Co., Germany) were stained with Hematolxylin & Eosin, followed by optical microscopy (Olympus, Tokyo, Japan) observed histological changes of hair follicle tissue.

In other words, the formation of hair follicles was evaluated by histological examination of the tissues at the 4th week of Ginsenosai F ₂ application.

Jinse nosayi F ₂ applied at 4 weeks the tissue of the application site evaluation, As can be seen in Figs. 7 and 8, the number of hair follicles a test group applied the 0.5% ginsenoside F ₂ as compared to negative and positive control group with a lot of size The appearance of hair follicles was increased, and no inflammatory or other pathological findings were found.

Experimental Example 5 Hair Loss Prevention and Hair Growth Effect Measurement

In order to examine the hair loss prevention and hair growth effects of Ginsenocyte F ₂ of Example 1, the following experiment was performed.

(1) Hair Regrowth Composition Preparation

A ginsenoside F ₂ -containing hair repellent composition was prepared in the hydrogel base according to the component contents of Table 3 below. Test groups 1 and 2 are hair regrowth compositions comprising ginsenoside F ₂ extracted from red ginseng and ginsenoside F ₂ extracted from fermented red ginseng, respectively.

(2) Hair loss prevention and hair growth effect measurement

Forty to late 60's hair follicles were contracted and 40 patients, including bald baldness alopecia, typical male alopecia patients, and acute alopecia areata, were divided into four groups. The hair growth inhibitor prepared in the above step 1 was applied to hair loss sites of hair loss patients twice a day for 3 consecutive 6 months. Hair loss and hair growth were observed on a monthly basis. As a comparative group, Moxidil (Hanmi Pharm), which is commercially available, was used and only 50% ethanol was used as a control group.

The criteria for evaluation are as follows.

4-high effect: visible newborn hair (bristles),

3-moderate effect: visible newborn hair (downy),

2-slightly effective: the number of hair loss is reduced,

1-No effect: no change at all.

The results are shown in Table 4 below.

As can be seen in Table 4 above, hair loss including bristles including new hair began to appear in the alopecia patients using the hair growth agent containing ginsenoside F ₂ of the present invention from the first or second month of treatment, and at the sixth month of treatment, 80 More than% of patients showed a hair growth effect. In addition, we observed that newborn babies grew continuously and no hair loss phenomenon was found.

Preparation Example 1 Preparation of Balm Tonic

Hair tonic having a composition as shown in Table 5 below was prepared according to a conventional method.

Preparation Example 2 Preparation of Hair Shampoo

To the hair shampoo having a composition as shown in Table 6 was prepared according to a conventional method.

[Example 3] Hair Conditioner

To prepare a hair conditioner having a composition as shown in Table 7 in accordance with a conventional method.

[Example 4] Hair Lotion

To prepare a hair lotion having a composition as shown in Table 8.

[Example 5] Hair Soap

To prepare a hair soap having a composition as shown in Table 9.

Claims (6)

External composition for promoting hair growth or preventing hair loss containing ginsenoside F ₂ of Formula 1 as an active ingredient.

The method of claim 1,
Ginsenoside F ₂ of Formula 1 is An external preparation composition, which is attributable to hair follicle cell activation ability. 3. The method of claim 2,
Ginsenoside F ₂ of Formula 1 is an external composition, characterized in that to promote the growth of hair follicle cells. The method of claim 1,
Ginsenoside F ₂ of Formula 1 is an external composition, characterized in that extracted from fermented red ginseng. The method of claim 1,
The active ingredient is an external composition, characterized in that it comprises 0.001 to 50% by weight relative to the total weight of the composition. 6. The compound according to any one of claims 1 to 5,
The composition includes hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nourishment cream, hair moisturizer cream, hair massage cream, hair wax, hair aerosol, Hair Pack, Hair Nutrition Pack, Hair Soap, Hair Cleansing Foam, Hair Oil, Hair Dryer, Hair Preservative, Hair Dye, Hair Wave Agent, Hair Bleach, Hair Gel, Hair Glaze, Hair Dresser, Hair Lacquer, Hair Moisturizer, Hair External preparation composition which is a formulation of mousse or hairspray.

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