KR20110067529A - Cosmetic composition comprising novel black ginseng without tumor promoting agents such as benzopyran showing hair-growth stimulating activity and preventing activity from hair loss - Google Patents

Abstract

PURPOSE: A cosmetic composition and a method for manufacturing the same are provided to ensure rich sponin content without carcinogen. CONSTITUTION: A cosmetic composition for preventing hair loss and promoting hair growth contains black ginseng extract as an active ingredient. A method for manufacturing the extract comprises: a step of drying ginseng at 20-60°C for 24-96 hours; a step of steaming the ginseng at 0.06-0.50MPa and 105-155°C for 1-4 hours; a step of drying at 40-70°C for 24-48 hours to have 14% or less of moisture content; a step of pulverizing the ginseng and adding 3-7(w/v) times of ginseng; a step of placing at room temperature for 3-7 hours and adding a solvent; and a step of extracting the ginseng at 80-120°C by enfleurage, hot water extraction, sonication, and reflux extraction for 1-10 hours.

Description

Cosmetic composition having anti-hair loss and hair growth promoting effect containing black ginseng extract prepared by the novel manufacturing method {as cosmetic composition comprising novel black ginseng without tumor promoting agents such as benzopyran showing hair-growth stimulating activity and preventing activity from hair loss}

The present invention is shorter than the conventional black ginseng manufacturing method through the process of repeating drying and steaming at the appropriate temperature and time from ginseng, using high temperature and high pressure capable of mass production Rg ₃ , Rk ₁ , Rg ₅ , Rh ₁ , contains a large amount of saponins not present in traces or red ginseng, such as Rh ₂ and red ginseng, and has a hair loss prevention and hair growth promoting effect containing black ginseng extracts of high quality, which is rarely detected carcinogenic substances such as benzopyran It relates to a cosmetic composition.

Reference 1 Okamura N. et al., Biol. Pharm. Bull. , 17 (2) , p.270, 1994

[Document 2] Republic of Korea Patent Registration No. 10-0543862

[Document 3] Republic of Korea Patent Registration No. 10-0157667

[Document 4] Republic of Korea Patent Registration No. 10-0161338

[Document 5] Republic of Korea Patent Registration No. 10- 0259037

[Document 6] Republic of Korea Patent Registration No. 10- 0260673

[7] Nam, SY, Mun, J. H, Yun, Y.W., Baek, IJ and Yon, J. M, Effect of NDM hair tonic on hair growth promotion in an alopecia model of C57BL / 6 Mice, Korean J. Lab. Animal Sci ., 20 (1), 55 (2004)

Korean red ginseng has a historical record of about 1,000 years ago (there is a record of red ginseng in Goryeo-do, written by the Chinese Song Dynasty), and it is a numerical medicine processed and manufactured by numerical method, which is a traditional Chinese medicine. have. In general, the numerical objectives of herbal medicines are known as: 1) reducing side effects such as toxicity, 2) altering herbal properties and enhancing drug efficacy, 3) improving storage and storage, and 4) correcting taste and odor and color. In general, red ginseng undergoes a second ingredient conversion during an appropriate heat treatment process, thereby generating new active ingredients unique to red ginseng or fresh ginseng, and some active ingredients increase in content. As such, new ginsengs that do not exist in ginseng are produced according to the processing method of ginseng, and new processed ginseng can be developed that shows better effects on various physiological activities including anticancer activity than ginseng (Okamura N. et al., Biol. Pharm. Bull. , 17 (2) , p. 270, 1994).

In the case of China, it developed a method of mass production of Rg ₃ , a ginseng saponin, which is rarely present in white ginseng and is present in small amounts in red ginseng, and is used for anticancer treatment under the trade name Shenyi.

In addition, domestic bio venture companies are working hard to develop new ginseng-related materials to capture the $ 3.5 billion global ginseng market. Until now, only Palmerton, a subsidiary of Boehringer Ingelheim, Switzerland, has standardized ginseng as a functional food and is making progress in the global market. In Korea, national research on ginseng and commercialization of the venture industry are in progress.

Hwangsam EX, co-developed by Korea Research Institute of Biotechnology and biotechnology company Beatijin, Panghwa Red Ginseng, a product of Nam-il Farm Co., Ltd. Several types of processed ginseng, such as Biomax, CJ Co., Ltd. Six Plus, and Ildong Pharmaceutical's Yellow Ginseng, are being developed.

In addition, recently, the principle of Gujeunggupo (Korean Patent Registration No. 10-0543862) black ginseng, which is the most representative method, is to use water and fire together in the processing method of Chinese herbal medicine, one of the steaming methods. There is a bar.

However, the above method may cause problems such as carbonization in the process of steaming and drying nine times. In fact, carcinogenic substances such as benzopyrans are detected in black ginseng prepared in such a method, causing social controversy. In addition, if the black ginseng is manufactured under the conditions of high temperature and high pressure without drying the ginseng, the fresh ginseng may burst during the manufacturing process, and thus, black ginseng of excellent quality cannot be prepared.

Therefore, the present inventors use the ginseng to reduce the time, compared to the method of apocytic vesicles, mass production is possible, not burst, not carbonized, and saponins such as Rg ₃ , Rk ₁ , Rg ₅ , Rh ₁ , Rh _2, etc. A patent application (Korean Patent Application No. 10-2009-0048666) has been completed by completing a manufacturing method for producing black ginseng of high quality containing a large amount.

On the other hand, human hair has a hair cycle, and after a certain period of time comes to rest from the scalp and fall out irrespective of the hair cycle is called alopecia, also called toxicosis (禿髮 症) or poisoning disease (병). In other words, it is a condition in which abnormal hair loss or hair is not present or is poor. Not only the hair but also all the hair stops growing after a certain period of growth and enters the resting phase, and the hair is repeatedly lost to repeat the hair growth. This is called the hair growth cycle. The growth cycle of eyebrows, eyelashes, and fluffy is less than 6 months. On the other hand, hair growth is longer than 2-6 years, resting period is shorter than 2 ~ 3 months, and each has an independent growth cycle. Adults have 2 to 5% of their hair at rest. The hairs at rest are lightly pigmented, glossy, hairy, and easily pulled off with hair and comb. In addition, fever disease, pregnancy, mental stress, such as the growth phase of the hair suddenly enters the resting period, a lot of missing, the cause is eliminated and is recovered. The causes of hair loss known to date include excessive sebum production due to abnormal circulation, abnormal circulation, blood circulation disorders, nutrient deprivation of hair roots, physical factors such as allergies and bacterial infections, genetic factors such as the effects of male hormones, various pollution and processing. It is caused by a combination of environmental factors such as food, shampoo, soap, mousse, hair gel, hair spray, perm, dyeing, and hair dryer, and psychosocial factors of modern people who are frequently stressed, and aging. It is assumed to be. In recent years, most hair loss patients have been found to be responsible for hair loss. Demodex is a parasitic mite belonging to spiders and aphids, which live on the sebaceous glands and hair follicles of human eyelids, around the nose, the ear canal, and the head. Among the conventional hair growth promoting drugs for treating such alopecia, oral finasteride (Finasteride, Merk, USA) and external preparation minoxidil (Minoxidil, Upjohn, USA). Minoxidil has a capillary dilation function that prevents hair loss by applying it to the scalp, but it must be applied twice a day continuously, and only after the drug stays on the scalp for 3 to 4 hours for some effect. In terms of the effect is inadequate, side effects such as itching, irritation appear. Finasteride (Finasteride) has the effect of inhibiting the production of 5α-dihydrotestosterone (DHT), a male hormone that causes hair loss, and is known to have a hair growth or hair loss inhibitory effect when taken for 6 months to 1 year. There was a problem returning to the original state. In addition, side effects may include decreased sexual function, such as decreased libido, erectile dysfunction, etc. Women are known to not take because of the risk of birth defects.

       As described above, the above-mentioned hair restorers have problems such as economic burdens and pains to hair loss patients or cause side effects, use of only temporary or fragmentary pharmacological mechanisms, and the effects are more excellent and persistent. There is a growing demand for drugs that do not have side effects.

       There have also been many attempts to promote hair growth by applying or taking herbal medicines to the scalp, using the extract of orchid plants (Korean Patent No. 0157667), and using the wormwood and mandrel (Korean Patent No. 0161338). ), Half, clove, Bokbunja, Sancho, Manza, Dansam and white porcelain (Korean Patent No. 259037), mungbean flour, soybean flour, black rice flour, perilla sesame powder, sesame powder, walnut powder, laver powder, mussel powder, Oyster powder, anchovy powder, seaweed powder, kelp powder, yeast concentrate, or a mixture of dried powder thereof (Korean Patent No. 0260673) has been published, but its principle of action is not clear and its efficacy has not been sufficiently verified. to be.

Therefore, the present inventors use the ginseng to reduce the time, compared to the method of apocytic vesicles, mass production is possible, does not burst, not carbonized, saponins such as Rg ₃ , Rk ₁ , Rg ₅ , Rh ₁ , Rh ₂ The black ginseng extract of high quality containing a large amount was prepared and tested for the actual hair growth promoting effect using the C57BL / 6 mouse on black ginseng extract prepared by such a manufacturing method. The invention has been completed.

In order to achieve the above object, the present invention contains a black ginseng extract prepared by a novel method of manufacturing black ginseng using high temperature and high pressure, which can be shortened in time through a process of drying, steaming, heating and drying ginseng. It provides a cosmetic composition having a hair loss prevention and hair growth promoting effect.

     Extracts as defined herein are water, including purified water, lower alcohols having 1 to 4 carbon atoms or mixed solvents thereof, hexane, butylene glycol, propylene glycol, glycerin, ethyl acetate, ethers, chloroform, preferably water It is characterized in that the extract or the irradiated phonophonic extract as follows.

The cosmetic composition is a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisturizing cream, hair massage cream, hair wax, hair aerosol , Hair pack, nourishment pack, hair soap, hair cleansing foam, hair oil, hair drier, hair preservative, hair dye, hair wave, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, Formulations of hair mousse or hairspray.

Black ginseng and black ginseng extract of the present invention can be prepared as follows.

According to the present invention, the ginseng is washed with an ultrasonic cleaner and dried at a temperature of 20 to 60 ° C., preferably 45 to 60 ° C., for 24 to 96 hours, preferably 30 to 48 hours, so that the moisture content is 20 to 55%. Preferably, the first step of performing a first drying process to be 25 to 35%; Performing a steaming process for 1 to 4 hours, preferably 2 to 3 hours at a temperature of 105 to 155 ° C, preferably 110 to 135 ° C under a pressure of 0.06 to 0.50 MPa, preferably 0.10 to 0.25 MPa. Second step; A third step of performing a secondary drying step of drying within a moisture content of 14% for 24 to 48 hours, preferably 30 to 40 hours at a temperature of 40 to 70 ° C, preferably 50 to 60 ° C; A fourth step of grinding the black ginseng prepared in the third step, adding about 3 to 7 times, preferably 4 to 6 times (v / w) of water to the black ginseng, and leaving it at room temperature for 3 to 7 hours; Thereafter, water containing purified water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, a solvent selected from hexane, butylene glycol, propylene glycol, glycerin, ethyl acetate, ether, and chloroform is preferably added to water. Extraction method such as cold sedimentation, hot water extraction, ultrasonic extraction, reflux cooling extraction for 10 hours, preferably 2 to 6 hours at 80 to 120 ℃, preferably reflux cooling and then cooled to room temperature, filtered and reduced pressure The black ginseng extract of the present invention may be obtained through a manufacturing method including a fifth step of removing a solvent using a concentrator.

In addition, the black ginseng extract is suspended by adding water, and then a nonpolar solvent such as hexane, ethyl acetate, chloroform, ether, and the like is added to remove the nonpolar substance by adding ether and saturated saponin to the remaining water layer to add saponin. The crude saponin extract containing more ginseng saponin can be obtained through an additional step of extracting and removing the solvent with a vacuum condenser.

The black ginseng prepared by the preparation method has a large amount of saponins such as Rg ₃ , Rk ₁ , Rg ₅ , Rh ₁ , and Rh ₂ present in trace amounts in red ginseng. Among the saponin components, Rg ₅ has various pharmacological activities such as anticancer effect, antidiabetic effect, and Rg ₃ has anti-stress effect, hepatoprotective effect and antioxidant effect. Can be. In addition, as a result of measuring the content of benzopyran, a carcinogenic substance present in the prepared black ginseng, it was confirmed that it is detected at 2ppb or less.

The production method of the present invention can be applied to ginseng leaves as well as ginseng itself. Ginseng leaves have not been used for medicinal purposes, but have been discarded or used for feed, and some of them have been used as raw materials for cosmetics or foods.However, when ginseng leaf extract is heated and pressurized in the same way as in the present invention, Since it is much strengthened, it can be used for medicinal purposes as well as the use of ginseng leaves.

The black ginseng and the extract prepared by the above method are characterized in that the existing ginseng, white ginseng, or red ginseng does not exist or enhances the content of the active ingredient present in trace amounts.

Accordingly, the present invention provides a cosmetic composition having a hair loss prevention and hair growth promoting effect containing the black ginseng extract or the irradiated pohnponin extract obtained by the above production method as an active ingredient.

The composition of the present invention comprises 0.01 to 10% by weight, preferably 0.1 to 8% by weight and most preferably 0.5 to 5% by weight, based on the total weight of the composition.

Black ginseng extract obtained by the above production method was confirmed that the hair growth effect through the mouse.

The composition containing the extract of the present invention can be used in various ways, such as cosmetics and shampoos for hair loss prevention and hair regrowth effect. Examples of products to which the composition can be added include various softening creams, nourishing creams, nourishing creams, massage creams, essences, packs, hair tonics, hair lotions, shampoos, rinses, soaps, and the like.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. , Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenylethyl) Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend with the cosmetic composition of this invention the other component normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate decyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapate, capric acid Propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylic acid glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, neopentane dodecyl Isostearyl octanoate, octyl isononate, hexyl decyl neodecanoate, octyl dodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5% weight with respect to gross weight, More Preferably 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

Functional cosmetic composition for preventing hair loss and promoting hair growth of the present invention can be prepared in any formulation commonly prepared in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, essence, hair cosmetics, etc. Can be mentioned.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

As described above, the composition for preventing hair loss and promoting hair growth containing the black ginseng extract of the present invention shows a hair growth effect through the mouse without any side effects or toxicity to the human body, and thus can be used in cosmetic compositions showing the hair loss prevention and hair growth promoting effect. Can be.

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Comparative Example 1. Preparation of Gujeunggu Black Ginseng Extract

1kg of 5 year old ginseng grown in Geumsan was purchased (Gumsan Ginseng Combination), put into an ultrasonic cleaner and washed repeatedly three times for 15 minutes. The washed ginseng was put into a steamer and steamed for 1 hour at 95 ° C except for the preheating time. The preheating time is typically 30 minutes, which is the time from the steamer to the water vapor, and after maintaining the internal temperature of the dryer with built-in heating wire at 60 ℃, rotate the pen evenly for the first time. Tea was dried.

By repeating the steaming and drying process 9 times as described above, a black ginseng having a water content of 14% or less was changed to black through white ginseng.

10 g of the black ginseng prepared above was taken, extracted three times by the following extraction method, and injected into HPLC for analysis. That is, 10 g of black ginseng was weighed and mixed with 100 ml of hexane. Then, 50 ml of water: DMF mixed solution (1: 9) was added and shaken uniformly to separate DMF and hexane. 25 ml of DMF mixture was added to the hexane layer and extracted. This operation was repeated twice to bring the total DMF to 100 ml. 100 ml of 1% sodium sulfate solution was added to the separated DMF mixed layer, and the mixture was sufficiently mixed. Then, 50 ml of hexane was added thereto, followed by extraction. The hexane layer was washed three times with distilled water, dehydrated with anhydrous sodium sulfate, and concentrated under reduced pressure at 40 ° C. to use as a sample of carcinogenic substance (hereinafter referred to as GS).

Comparative Example 2. Using high temperature and high pressure directly in fresh ginseng Preparation of Black Ginseng

1kg of 5 year old ginseng grown in Geumsan was purchased (Gumsan Ginseng Combination), put into an ultrasonic cleaner and washed repeatedly three times for 15 minutes. The washed ginseng was put into a steamer and steamed for 3 hours at 119 ° C under pressure (0.13 MPa) except for the preheating time. The preheating time is usually 30 minutes from the steamer to the water vapor, and the tissue was destroyed because the ginseng was pressed without drying during steaming, and the active ingredient, such as saponin, flowed out of the tissue. . After maintaining the internal temperature of the dryer with a built-in heating wire at 60 ℃, the first ginseng evenly steamed while rotating the pen was first dried for 12 hours.

Comparative Example 3. Preparation of Red Ginseng Extract

1kg of 5 year old ginseng grown in Geumsan was purchased (Gumsan Ginseng Combination), put into an ultrasonic cleaner and washed repeatedly three times for 15 minutes. The washed ginseng was put into a steamer and steamed for 3 hours at 95 ° C except for the preheating time. The preheating time is usually 30 minutes, which is the time from the steamer to the water vapor.The red ginseng is dried for 48 hours while keeping the internal temperature of the dryer with built-in wire at 55 ℃ and rotating the pen evenly. 256 g was obtained.

50 g of the prepared red ginseng was pulverized, and 1L of 80% ethanol was added and refluxed three times for 4 hours. The extracted solution was cooled to room temperature, filtered, and the solvent was removed using a reduced pressure concentrator to obtain 19 g of red ginseng crude extract. 300 ml of water was added to the red ginseng crude extract and suspended. Then, 300 ml of ether was added to remove the nonpolar substance. 300 ml of saturated butanol was added to the remaining aqueous layer to extract saponin present in ginseng, and the butanol was removed using a vacuum concentrator. 4.8 g of red ginseng extract containing a large amount of ginseng saponin was obtained and used as a sample for measuring saponin content (hereinafter referred to as RGS).

Example 1 Preparation of Black Ginseng

1kg of 5-year-old ginseng grown in Geumsan was purchased (Gumsan Ginseng Combination), put into an ultrasonic cleaner and washed repeatedly three times for 10 minutes. The washed ginseng was first dried at 55 ° C. for 36 hours to prevent tissue from bursting during the manufacturing process of black ginseng, as in Comparative Example 2, so that the moisture content was 30%. First, the ginseng with 30% moisture content is steamed again (0.13MPa) at 119 ° C for 3 hours, and then dried for 2 hours at 55 ° C with a dryer for 2 hours. Was prepared.

10 g of the black ginseng prepared above was taken, extracted three times by the following extraction method, and injected into HPLC for analysis. That is, 10 g of black ginseng was weighed and mixed with 100 ml of hexane. Then, 50 ml of water: DMF mixed solution (1: 9) was added and shaken uniformly to separate DMF and hexane. 25 ml of DMF mixture was added to the hexane layer and extracted. This operation was repeated twice to bring the total DMF to 100 ml. 100 ml of 1% sodium sulfate solution was added to the separated DMF mixed layer, and the mixture was sufficiently mixed. Then, 50 ml of hexane was added thereto, followed by extraction. The hexane layer was washed three times with distilled water, dehydrated with anhydrous sodium sulfate, and concentrated under reduced pressure at 40 ° C. to use as a sample of carcinogenic substance (hereinafter referred to as NGS).

Example 2. Preparation of Black Ginseng Extract

Take 20 g of black ginseng prepared by the method of Example 1, add 100 ml of water, and leave it at room temperature for 5 hours, extract 1 ml of 80% ethanol (20% water) and reflux 3 times for 3 hours to extract the extracted solution. After cooling to room temperature and filtered, ethanol was removed using a vacuum concentrator to obtain a black ginseng extract (5.76g). 500 ml of water was added to the black ginseng extract and suspended, and 500 ml of ether was added to remove the nonpolar material. 500 ml of saturated butanol was added to the remaining aqueous layer, followed by extraction of saponin present in ginseng four times, followed by removing butanol with a reduced pressure concentrator to obtain a butanol extract (crude saponin) containing a large amount of ginseng saponin (1.98 g).

This black ginseng crude saponin extract was used as a sample for saponin component analysis (hereinafter referred to as NGSS).

Experimental Example 1. Analysis of saponin content of black ginseng

The saponin content of the red ginseng extract (RGS) obtained in Comparative Example 3 and the black ginseng saponin extract (NGSS) obtained in Example 2 was analyzed in the HPLC analysis conditions of Table 1 below, and the results are shown in the following table. 2 is shown.

Column: Shiseido C18 column (5㎛, 150 × 4.6mm) Flow rate 1ml / min Detector ELSD-LT detector Solvent A; CH ₃ CN: H ₂ O: 5% CH ₃ COOH = 15: 80: 5, B; CH ₃ CN: H ₂ O = 80:20
0 minutes (0% B), 0-10 minutes (30% B), 10-25 minutes (50% B), 25-40 minutes (100% B), 40-50 minutes (100% B), 50- 55 minutes (0% B), 55-58 minutes (0% B) Temperature 40 degrees

RGS (Comparative Example 3)% NGSS (Example 2)% Rg ₃ 0.066 0.98 Rh ₁ 0.069 0.094 Rh ₂ 0.051 0.085 Rk ₁ + Rg ₅ 0.075 1.19

As a result, as shown in Table 2, red ginseng contained a small amount of Rg ₃ , Rh ₁ , Rh ₂ and Rk ₁ + Rg ₅ as 0.066%, 0.069%, 0.051% and 0.075%, respectively. In black ginseng, Rg ₃ , Rh ₁ , Rh _2, and Rk ₁ + Rg ₅ were 0.98%, 0.094%, 0.085%, and 1.19%, respectively, and Rg ₃ and Rk ₁ + Rg ₅ were 15 times more present.

Experimental Example 2. Analysis of benzopyran content, a carcinogenic substance present in black ginseng

2-1. Analyzer condition

HPLC was used for the Waters alliance system, excitation wavelength 294nm, fluorescence wavelength 404nm, column temperature was 30 ℃, acetonitrile: tertiary distilled water (80:20) as a mobile phase, the flow rate was quantified at 1.0 mL / min.

2-2. Linearity, Detection Limit (LOD) and Quantitative Limit (LOQ)

50 mg of benzopyrene standard is precisely measured, dissolved in methanol to make exactly 50 ml, and then diluted to prepare a standard stock solution. Diluted to prepare a standard solution for each concentration of 7.8125, 15.625, 31.25, 62.5 and 125 ng / ml, and the peak area according to the analysis conditions. The calibration curve was prepared by obtaining. From the calibration curve, the linear correlation coefficient (standard: R ² = 0.99 or more) was obtained, and the linearity was examined.

The detection limit (LOD) and the quantitative limit (LOQ) were calculated using the following equations (1) and (2) by using a portion having a good linearity range of the calibration curve.

Detection limit (LOD) = 3.3 × σ / S (S / N = 3)

Limit of Quantitation (LOQ) = 10 × σ / S (S / N = 10)

(σ is the mean standard deviation of the intercepts, and s is the mean of the slopes.)

Benzopyran concentration (ug / Kg, ppb) GS (Comparative Example 1) 2.34 ± 0.065 NGS (Example 1) 0.32 ± 0.007

As a result of analyzing the content of benzopyran, a carcinogenic substance present in the black ginseng extract, as shown in Table 3, the black ginseng (GS) prepared by the method of erythropoietic cell in Comparative Example 1 exceeded 2 ppb as a reference value. Benzopyran was detected. On the other hand, black ginseng (NGS) prepared by using the method of high temperature and high pressure in Example 1, it was confirmed that benzopyran is detected below the reference value of 0.32ppb.

From the above results, the black ginseng (NGS) prepared by the method of the present invention not only kept the original shape of ginseng without bursting during the manufacturing process, and almost no carcinogenic substance such as benzopyran, which was recently controversial, was not detected. It was confirmed that black ginseng of good quality could be prepared.

Experimental Example 3. Hair Growth Promotion Experiment

In order to confirm the hair growth effect of the black ginseng extract of the present invention obtained in the above example, the experiment was performed by applying Lee et al. [9] method disclosed in the literature (Nam, SY, Mun, J. H, Yun, Y W., Baek, IJ and Yon, J. M, Effect of NDM hair tonic on hair growth promotion in an alopecia model of C57BL / 6 Mice, Korean J. Lab.Animal Sci ., 20 (1) , 55 (2004 ))

3-1. Preparation of Experimental Animals

    Five-week-old male C57BL / 6 mice were purchased from a central laboratory animal and used for experiments after being adapted to the animal breeding environment for one week. Animal breeding room was maintained at 23 ± 3 ℃ temperature, 50 ± 10% relative humidity, and 12 hours each day. The experimental animals were freely fed the feed and water, and the total of 6 groups was 6, and 6 animals were used for the experiment.

3-2. Experiment process

     To examine the wool effect, we used 6-cycle age of resting hairs with pink dorsal skin color. After depilation, the hair of the back of the mouse was removed using Evacrine depilatory drug (Sampo Pharmaceutical Co., Ltd.), and then, two days later, 100 μl of the sample was applied to each of the groups. Sample treatment was conducted once every two weeks at 10 am.

     As a sample, 3% MXD (minoxidil, Hyundai Pharmaceutical) and 5% black ginseng extract (sample dissolved black ginseng at 5% concentration in saline solution) were applied to the skin (hair removal area) at 100ul every day. Saline was applied.

   On the 1st, 7th and 14th day after the start of the experiment, light anesthesia was performed with ether to visually check the hair growth state, and the photo was taken with a camera (Nikon, Coolpix S230). Hair growth was visually evaluated by comparing 0-25% (-), 25-50% (±), 50-75% (+), 75-100% (++).

As a result, as shown in Figures 1 to 3, it was confirmed that the effect (++) of the black ginseng extract of the present invention showed an equivalent to superior effect than the hair growth effect of the minoxidil of the existing hair regrowth agent (see Table 4).

Comparison of hair re-growths in alopecia models of C57BL / 6 mice induced by Minoxidil after topical application of test compounds for 2 weeks Treatment Score Saline ± 3% minoxidil ++ 5% Black Ginseng Extract ++

Examples of the formulation of the composition containing the extract of the present invention will be described, but the present invention is not intended to be limited thereto but merely to be described in detail.

Formulation Example 1 Preparation of Hair Tonic

NGSS 1%

Ethanol 55.0%

Castor Oil 5.0%

Glycerin 3.0%

Pyroctoneamine 0.1%

Spices, pigments

Purified water level

Total 100.0%

Formulation Example 2 Preparation of Hair Lotion

NGSS 1.0%

Cetostearyl alcohol 2.0%

Stearyltriethylammonium Chloride 2.0%

Hydroxyethyl cellulose 0.5%

Pyroctonolamine 0.1%

Fragrance, pigment 0.5%

Purified water level

Total 100.0%

Formulation Example 3 Preparation of Hair Soap

NGSS 0.1%

Titanium dioxide 0.2%

Polyethylene Glycol 0.8%

Glycerin 0.5%

Ethylenediaminetetraacetic Acid 0.05%

Sodium 1.0%

Color, soap flavor

Cosmetic soap base (13% moisture, parts by weight)

Total 100.0%

1 is a diagram showing the hair growth effect of the mice coated with the control group (saline treatment) (A1-1 days; A2-7 days and A3-14 days);

2 is a diagram showing the hair growth effect of mice coated with a positive control (minoxidil treatment) (B1-1 day; B2-7 days and B3-14 days);

Figure 3 is a diagram showing the hair growth effect of the mice coated with the sample group (black ginseng extract treatment) (C1-1 day; C2-7 days and C3-14 days).

Claims (4)

A first step of washing the ginseng with an ultrasonic cleaner and then performing a first drying process for drying at a temperature of 20 to 60 ° C. for 24 to 96 hours so that the water content is 20 to 55%; A second step of performing a steaming step of steaming at a temperature of 105 to 155 ° C. for 1 to 4 hours under a pressure of 0.06 to 0.50 MPa; A third step of performing a secondary drying step of drying the water content within 14% for 24 to 48 hours at a temperature of 40 to 70 ° C; A fourth step of pulverizing the black ginseng prepared in the third step, adding about 3 to 7 times (v / w) of water to the black ginseng and leaving it to stand at room temperature for 3 to 7 hours; Thereafter, water containing purified water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, and a solvent selected from hexane, butylene glycol, propylene glycol, glycerin, ethyl acetate, ether, and chloroform were added thereto for 1 to 10 hours, and 80 to 80 After extraction by cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, etc. at 120 ℃ through a manufacturing method comprising a fifth step of cooling the solvent to room temperature, filtering and then removing the solvent using a vacuum condenser A cosmetic composition for preventing hair loss and promoting hair growth, comprising the obtained black ginseng extract as an active ingredient. The method of claim 1, wherein in addition to the fifth step, the black ginseng extract is suspended in water, and then hexane, ethyl acetate, chloroform, or an ether solvent is added to remove the non-polar substance, and saturated butanol is added to the remaining water layer to present the ginseng. A cosmetic composition for preventing hair loss and promoting hair growth, comprising a crude saponin extract obtained through an additional step of extracting saponin and removing the solvent with a reduced pressure concentrator as an active ingredient. The cosmetic composition according to claim 1 or 2, wherein the extract is contained in an amount of 0.01 to 5% by weight based on the total weight of the composition. According to claim 1 or 2, wherein the composition is a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisturizer cream, Hair Massage Cream, Hair Wax, Hair Aerosol, Hair Pack, Hair Nutrition Pack, Hair Soap, Hair Cleansing Foam, Hair Oil, Hair Dryer, Hair Preservative, Hair Dye, Hair Wave, Hair Decolorant, Hair Gel, Hair Glaze, Hair Dressing Uh, a cosmetic composition, characterized in that the formulation of the hair lacquer, hair moisturizer, hair mousse or hair spray.

Images