KR101496926B1 - Cosmetic composition for itch relief and anti-dandruff comprising the extract of Scutellaria baicalensis GEORGE, Coptis chinensis, Phelledendron amurense, Paeonia lactiflora and Rooibos - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of Scutellaria baicalensis George, Coptis chinensis, Phelledendron amurense, Paeonia lactiflora, and Rooibos for itch and dandruff prevention, and to a method for manufacturing the mixed extract of Scutellaria baicalensis George, Coptis chinensis, Phelledendron amurense, Paeonia lactiflora, and Rooibos. The mixed extract of Scutellaria baicalensis George, Coptis chinensis, Phelledendron amurense, Paeonia lactiflora, and Rooibos is contained in 0.001-10 wt% for total weight of the cosmetic composition. The mixed extract of Scutellaria baicalensis George, Coptis chinensis, Phelledendron amurense, Paeonia lactiflora, and Rooibos is preferred to have Scutellaria baicalensis George, Coptis chinensis, Phelledendron amurense, Paeonia lactiflora, and Rooibos be mixed in the same weight ratio and be extracted.

Description

[0001] The present invention relates to a cosmetic composition for preventing itching and improving dandruff, which contains a mixed extract of red, yellow, gold, yellow, white, pomegranate and rooibos Rooibos}

The present invention relates to a cosmetic composition containing a mixed extract of gold, golden, yellow, white, peony and rooibos. More specifically, the present invention relates to a cosmetic composition containing golden, yellow, white, To a cosmetic composition for hair.

The scalp has a wide distribution of marine and sebaceous glands, and it is a good environment for microbes to reproduce, and many microorganisms reside there. There are three main types of resident microorganisms: anaerobic bacteria, aerobic bacteria and fungus, Malassezia species. Dandruff occurs when Malassezia, one of these scalp bacteria, begins to reside in the same bore as the scalp after puberty, when male hormones are secreted. Particularly, due to environmental factors such as climate, sweat and food, and physiological factors such as stress, dandruff grows excessively, resulting in dandruff if it exceeds 74% of the total number of normal bacteria, and seborrheic dermatitis occurs when it exceeds 83%. As such, dandruff is a by-product of metabolism that occurs in living skin cells mixed with decomposition of sebaceous oats and decolorization of the sides of the aging of the scalp, which is a mild seborrheic dermatitis. However, when these symptoms become severe, it is called dandruff, clinically, tofu, and medically dry boredom. Scalp pain, itching, or inflammation or erythema accompanied by symptoms caused by dandruff must prevent the growth of dandruff must.

Also, caution should be exercised when excess dandruff is left to cause hair loss. Drugs and shampoos that have been used to treat dandruff include chemicals such as Zinc pyrithione, ketoconazole, piroctoneolamine, Selenium sulfide, and Coal tar solution. On the other hand, chemicals that have been widely used as anti-dandruff agents have a good antibacterial activity in a small amount. On the other hand, they are very dangerous substances that can be absorbed into the body and make the pregnant fetus malformed. It should be replaced by a natural substance that is not harmful to human body, but synthetic chemical. Chrysanthemum zawadskii, Phyllosrachys Bambusoides, Sanguisorbaofficinalis, Resina pini, Forsythiaviridissima and Echinops setifer have been reported to date as antidandruff effective plants .

Korean Patent Publication No. 2011-0058251 entitled " Anti-Dandruff Hair Cosmetic Composition "describes an anti-dandruff hair cosmetic composition further comprising at least one component selected from oat extract or the above extract of pine needles and ginkgo leaf extract . Korean Patent Laid-Open Publication No. 2013-0073579 entitled " Composition for improving skin and scalp condition containing an extract of natural product complex fermented extracts "includes " Oylum, A complex fermentation extract of a natural product, a preparation method thereof, and a composition for improving skin and scalp condition using the extract as an active ingredient.

There is a growing demand for the development of anti-dandruff and itch-relieving cosmetic compositions derived from natural materials which have no side effects even when used for a long period of time, unlike the conventional active ingredients and antifungal substances of hair cosmetics.

The inventors of the present invention have found that it is possible to prevent itching and improve dandruff when gold, yellow radish, yellow whites, peony root and rooibos are screened and the selected herbal medicines are mixed and extracted by searching for herbal medicine materials having excellent anti- Thus completing the present invention.

Accordingly, an object of the present invention is to provide a cosmetic composition containing a herbal medicine mixed extract having excellent anti-itch and dandruff activity.

Another object of the present invention is to provide a more effective method for producing a herbal medicine mixed extract having an excellent anti-itch and anti-dandruff activity.

In accordance with the present invention, there is provided a cosmetic composition for preventing itching and improving dandruff, which comprises a mixed extract of gold, yellow, white, peony, and rooibos. At this time, it is preferable that the mixed extract of gold, yellow, white, pomegranate and rooibos is obtained by ultrasonic extraction.

The combined extracts of the golden, yellow, white, peony, and roots are contained in an amount of 0.001 to 10% by weight based on the total weight of the cosmetic composition.

It is preferable that the above-mentioned mixed extract of gold, yellowtail, yellowtail, peony, and rooibus is mixed with the same weight ratio of the above-mentioned golden, yellowtail, yellowtail, peony and rooibos.

The cosmetic composition may be at least one selected from the group consisting of hair tonic, hair conditioner, hair essence, hair lotion, hair nourishing lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nourishing cream, hair moisturizing cream, , A hair pack, a hair nutrition pack, a hair soap, a hair cleansing foam, a hair oil, a hair gel, a hair mousse or a hair spray.

According to another aspect of the present invention, there is provided a method for manufacturing a gold-plated steel plate, comprising the steps of: mixing gold, rhodochrosite, At least one solvent selected from the group consisting of water in a volume of 1 to 50 times, an alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol is added and heated at 50 to 80 占 폚 1 to 2 hours; Leaving at room temperature for 7 to 10 days after filtration; And concentrating and drying after filtration, a method for producing a mixture of gold medallion, yellow whites, peonies and rooibos is provided. Preferably, the extraction solvent is 70% by weight ethanol and the extraction temperature is 80 占 폚.

The mixed extracts of golden, yellow, yellow, white, peony, and rooibos according to the present invention are excellent in antimicrobial activity against dandruff, suppression of sebum secretion, and reduction of pores, and the cosmetic composition containing the same is useful as a cosmetic for preventing itching and improving dandruff Can be used. great.

1 is a graph showing the content of bicalkine in the mixed extract of Example 11 of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention is characterized in that a mixed extract of golden, yellowtail, yellowtail, peony and rooibos is prepared and used as a cosmetic for hair.

Golden (黄 芩) is a herbaceous herbaceous plant belonging to the family Lamiaceae (Scutellaria baicalensis GEORGE) is the root of the drug. The golden (Scutellaria baicalensis GEORGE) grows in the grasslands of the mountains and is 20 to 60 cm in height. Several families grow up to give up, have hairs, and branches are divided. Leaves are opposite to each other and have a straight edge. The fruit is fruitful in September, and is round and shaped in calyx. The roots are conical and yellow in color. The roots are used as acupuncture, diuretic, branch, ejaculation, and anti-inflammatory in oriental medicine. On the other hand, bicalin, one of the flavonoids, is known to have antioxidant, anti-cancer and anti-inflammatory effects as a component contained in gold [Jung SH, Kang KD, Ji D et al. The flavonoid baicalin counteracts ischemic and oxidative insults to retinal cells and lipid peroxidation to brain membranes. Neurochem Int 2008 53: 325-337. Li-Weber M. New therapeutic aspects of flavones: the anticancer properties of scutellaria and its main active constituents wogonin, baicalein and baicalin. Cancer Treat Rev 2009 35: 57-89].

Hwangryeong (黄连) is a Chinese origin and is also used for medicinal purposes in Korea, Japan and China. It grows in the ecological land of the shade of the mountain forest. The underground stem is thick and spreads to the side. It has many beard roots, 4 ~ 5 roots at the end of stem, and 10 ~ 27cm in length. The leaf is a compound leaf with three small leaves. The small leaf is a little hard, sharp and sharp. It is named after the stem and the stem of the underground stem are dark yellow. It is called 黄连 (黄连), which is rooted in the sun for about 5 ~ 6 years after harvesting the roots of rootstocks, why they are planted in November, and has the effect of dryness, soothing, anti-inflammation and antibacterial.

Huangyak is a word that refers to the bark of a primrose tree as a medicinal herb, and it is also called Hwangkeongpyeong. It is a deciduous broad-leaved arboreous tree with a height of 10m. The shell has developed cork and the inner skin is yellow. Leaves are opposite to each other, with double leaf petiole, 5 ~ 13 petioles, egg-shaped lanceolate or long round. There are small sawtooth and soft hairs on the edge of the leaf, and there are some long hairs on the base of the middle vein. Yellow flowers bloom in May-June, which is a monotony of diatoms. It is distributed in all regions except Jeju and Jeonnam regions in Korea. It is distributed in Japan, Manchuria, China, Amur, and Sochi, and grows in forests or mountain valleys.

Peel off the trunk from the trunk before and after removing the bark (rough skin) and cut and dry in the sun. This shell is used as a medicine to lower blood sugar. It also inhibits growth of pneumococcal bacteria, Mycobacterium tuberculosis, Staphylococcus aureus, etc., inhibits the growth of tumor cells, and has a sterilizing action.

Paeonia lactiflora is a perennial herb that grows in mountainous areas. Several stems come out of a stand and stand straight, about 60cm high, with no hairs on leaves and stems. There are several roots, but they are thin and thick with a pointed cylindrical shape with both ends. Leaves are alternate and lower leaves are double leaves with three small leaves. Flowers bloom in May to June at the end of the stem. It is large and beautiful. It grows about 10cm in diameter. There are many kinds of horticultural varieties such as red and white. The fruit is ovate, the end is bent like a hook, it is split along the inner line, and the seed is spherical. The flowers are beautiful and they are used for gardening. Root is used as medicine for pain, abdominal pain, dysmenorrhea, amenorrhea, hematemesis, anemia, bruise.

Rooibos (Rooibos, Aspalathus linearis ) is a conifer belonging to legume plants. It grows only in the Cederberg Mountains, north of Cape Town, South Africa. The leaves are dried and used as a car. The tea is sweet, has no caffeine, has an extremely low tannin concentration, and is considered antioxidant.

According to the present invention, there is provided a cosmetic composition for preventing itching and improving dandruff, which contains a mixed extract of golden, yellow, white, peony and rooibos. According to one embodiment of the present invention, the ultrasound extraction method is applied to the preparation of the mixed extracts of the golden, yellow, white, peony and rooibos.

Ultrasonic extraction method is applied to obtain good efficiency in fast reaction time in pulverization, mixing and extraction processes. It has higher extraction efficiency, less energy consumption than heat extraction method which is the conventional traditional extraction method, It has the advantage of being stable against heat, while preventing destruction and loss of protein. Such an ultrasonic extraction method also has an advantage in that it can increase the efficiency of the extraction process and can obtain many physiologically active substances safely and effectively when applied to a herbal or natural herbal raw material. In the ultrasonic extraction method, the ultrasonic wave is a sound Refers to a sound of a high frequency that can not be heard by the ear, wherein the term "low temperature" refers to a temperature of 60 ° C. or less, and the plant mixture is generally designed by avoiding the extraction temperature at a high temperature or a cryogenic temperature to selectively remove unnecessary components, And overcome the drawbacks of inadequate selectivity of solvent by ultrasonic extraction method.

In addition, the ultrasonic extraction method is characterized in that the intensity of the ultrasonic energy change can be controlled according to the raw material to be extracted in the production of the plant extract. Therefore, the extraction efficiency is very high according to the raw material, and the possibility of denaturation and destruction of the active ingredient exhibiting pharmacological activity is minimized. The production speed is faster than the conventional hot water extraction method, thereby shortening the extraction time and using the low cost low energy. .

In addition, the extracts extracted by the ultrasonic extraction method have an excellent ultraviolet absorbing effect in comparison with the extracts extracted by the hot water extraction method and the solvent extraction method, and have a high non absorbance and can be used in various bases and additives for cosmetics, It is possible to provide a cosmetic composition which is excellent in solubility, affinity and stability, and protects the skin from various stresses and has an effect of moisturizing and cleansing the skin.

In the present invention, the extraction solvent is selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol, butylene glycol and mixed solvents thereof (for example, functional alcohols, Water, propylene glycol, and propylene glycol), and is preferably extracted with a solvent selected from water, anhydrous alcohol having from 1 to 4 carbon atoms, or hydrated alcohol. Here, the alcohol having 1 to 4 carbon atoms may be methanol, ethanol, n-propanol, iso-propanol, n-butanol, iso-butanol or tert-butanol. Ethanol is preferred. Here, the functional alcohol may contain 0.3 to 90 (wt / wt)% of water.

Hydroxy ethanol is most preferred in these extraction solvents, and the amount of water contained is preferably 30 to 90 (wt / wt)%, more preferably 70 (wt / wt)%. In the present invention, 00% functional alcohol means that the alcohol is contained at a weight ratio of 00%.

The mixed extract of golden, yellow, white, peony and rooibos of the present invention can be prepared by a known extraction method known to a person skilled in the art. For example, in the present invention, the dried products of gold, yellow, white, peony, and rooibos are chopped and selected from among 1 to 50 times as much volume of mixed dry weight of water, 1 to 4 carbon atoms or their hydrated alcohols Adding an extraction solvent and immersing the mixture at 4 to 30 DEG C for 3 to 20 days to extract an active ingredient and then concentrating the extraction solvent with a vacuum concentrator. In addition, the dried products of gold, yellow, white, peach, rooibos of the present invention are pulverized and mixed, and water, 1 to 30 times by volume of the mixed dry weight thereof, alcohol having 1 to 4 carbon atoms, ethyl acetate, water, glycerin , At least one solvent selected from the group consisting of ethylene glycol, propylene glycol, butylene glycol, and mixtures thereof is used. Then, in order to prevent evaporation of the solvent, an extractor equipped with a cooling condenser is used, Heating for 48 hours, or immersing the mixture at 5 to 30 DEG C for 1 to 5 days to extract the active ingredient, and concentrating the extraction solvent with a vacuum concentrator.

More preferably, it is prepared by the ultrasonic extraction method known in the above-mentioned extraction solvents.

It is preferable to extract the dried product of the golden, yellow, white, peony, and rooibos of the present invention with water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, and the extraction temperature may vary depending on the extraction solvent, .

The golden, yellow, white, peony, and rooibos extracts of the present invention may be a concentrate obtained by concentrating an extract obtained by extracting with an extraction solvent, a lyophilized concentrate or a powder obtained by spray drying and concentrating the concentrate.

In the cosmetic composition of the present invention, the mixed extract of golden, yellow, white, peony and rooibos of the present invention may be added to the cosmetic in an amount of 0.001 to 10.0% by weight based on the total weight of the cosmetic composition, preferably 0.001 to 5.0 % ≪ / RTI > by weight of the composition.

The cosmetic composition of the present invention is not particularly limited in its formulation, and examples thereof include hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, It may have formulations such as nutritional cream, hair moisturizing cream, hair massage cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair gel, hair mousse or hair spray.

In addition, in the cosmetic composition of each formulation, components other than the above-mentioned mixed extract of golden, yellow, white, peony and roots can be arbitrarily selected and formulated according to the formulation or purpose of use of other cosmetics. For example, it may include colorants (coloring matters), flavoring agents (flavoring agents), suspending agents, emulsifying agents, dissolution aids, stabilizers, isotonic agents, pH adjusting agents, viscosity adjusting agents, solvents, preservatives and the like which are conventionally used in cosmetic compositions . The cosmetic composition of the present invention may contain at least one other active ingredient which exhibits anti-itchiness and dandruff improvement, in addition to a mixed extract of gold, yellow, white, peony and rooibos.

[Example]

Hereinafter, the constitution and effects of the present invention will be described in detail with reference to examples, comparative examples and formulation examples, but the present invention is not limited thereto.

Examples 1 to 5: Powder preparation (hot water extraction) of each of the extracts of golden, yellow, white, peony, and rooibos,

200g each of dried gold, yellow, white, peony and rooibos was washed with purified water, and 2kg of distilled water was added to the dried gold, yellow, white, peony, and rooibos purchased from Kyungdong Market. The mixture was filtered through a 300-mesh filter paper, left at room temperature for 1 week, and the precipitate was filtered through Edbentek No. 5 filter paper and Wattman GF / C 150 mm filter paper twice. The concentrate was concentrated at a temperature of 40 to 50 ° C using a vacuum concentrator and then dried to obtain an extract powder of the title using a spray drier (model name: B-290, manufactured by BUCHI) under the following conditions (Krussmann, 1982 ). The results are shown in Table 1 below.

Example 6 Preparation of Powder of Golden, Yellow, White, Peony and Rooibos Mixed Extract (Hot Water Extraction)

The powder was prepared by mixing, extracting and filtering in the same manner as in Example 1, so as to obtain 200 g of a mixture of each of 40 g each of 1: 1: 1: 1: 1 ratio of gold, The results are shown in Table 1 below.

Example 7 Preparation of Powder of Golden, Yellow, White, Peony and Rooibos Mixed Extract (Ultrasonic Low Temperature Extraction)

After washing and drying the roots, golden, yellow, white, peony, and roots were added to 2 kg of distilled water in an amount of 1: 1: 1: 1: , And the mixture was allowed to stand at room temperature for 1 week. The precipitate was filtered twice using a filter paper of Edbentek No. 5 and a Wattman GF / C 150 mm filter. The concentrate was concentrated at a temperature of 40 to 50 ° C using a vacuum concentrator and then dried to obtain an extract powder of the title using a spray drier (model name: B-290, manufactured by BUCHI) under the following conditions (Krussmann, 1982 ). The results are shown in Table 1 below.

Example 8 Preparation of Powder of Golden, Yellow, White, Peony and Rooibos Mixed Extract (Ultrasonic High Temperature Extraction)

After washing and drying the roots of golden, yellow, white, peony, and roots, the mixture was added to 2 kg of distilled water in a ratio of 1: 1: 1: , And the mixture was allowed to stand at room temperature for 1 week. The precipitate was filtered twice using a filter paper of Edbentek No. 5 and a Wattman GF / C 150 mm filter. The concentrate was concentrated at a temperature of 40 to 50 ° C using a vacuum concentrator and then dried to obtain an extract powder of the title using a spray drier (model name: B-290, manufactured by BUCHI) under the following conditions (Krussmann, 1982 ). The results are shown in Table 1 below.

division Powder (g) Example 1 15 Example 2 14 Example 3 13 Example 4 8 Example 5 12 Example 6 15 Example 7 12 Example 8 16

Examples 9 to 12: Preparation of Powder of Golden, Yellow, White, Peony and Rooibos Mixed Extracts by Extraction Solvent (Ultrasonic High Temperature Extraction)

After washing and drying the roots of golden, yellow, white, peony, and rooibos, each of 40g of the mixture was mixed with 200g of the mixture at a ratio of 1: 1: 1: The mixture was filtered through 300 mesh filter paper, left at room temperature for 1 week, and the precipitate was filtered twice with filter paper Edbentek No. 5 and Wattman GF / C 150 mm filter paper. The concentrate was concentrated at a temperature of 40 to 50 ° C using a vacuum concentrator and then dried to obtain an extract powder of the title using a spray drier (model name: B-290, manufactured by BUCHI) under the following conditions (Krussmann, 1982 ). The results are shown in Table 2 below.

COMPARATIVE EXAMPLE 1 Preparation of Powder of Golden, Yellow, White and Peony Mixture Extract (Ultrasonic High Temperature Extraction)

After washing and drying, four kinds of prescription ingredients of Seiho Cheongsan San acid were added to 2kg of 70% ethanol. The mixture was mixed with 1: 1: 1: 1 at a ratio of 1: 1: 1: C. for 2 hours, filtered through a 300-mesh filter paper, left at room temperature for 1 week, and the precipitate was filtered twice with a filter paper of Edbentek No. 5 and Wattman GF / C 150 mm filter paper. The concentrate was concentrated at a temperature of 40 to 50 ° C using a vacuum concentrator and then dried to obtain an extract powder of the title using a spray drier (model name: B-290, manufactured by BUCHI) under the following conditions (Krussmann, 1982 ). The results are shown in Table 2 below.

division Extractor Yield (g) Example 9 30% functional ethanol 16 Example 10 50% functional ethanol 17 Example 11 70% functional ethanol 20 Example 12 80% functional ethanol 19 Comparative Example 1 70% functional ethanol 19

As shown in Table 1 and Table 2, more mixed extracts than the single extract were obtained, and ultrasonic extraction was obtained more than ultrasonic extraction (high temperature) rather than hot water extraction. % Function ethanol is better.

Test Example 1: Antimicrobial activity test for dandruff

The activity against five Malassezia species was measured for Examples 1 to 12 above. Microbroth dilution test was performed to select excellent natural substances with antimicrobial activity against 5 species of Malassezia. The composition of the medium for evaluating the antimicrobial activity of the natural extract is 10 g / L of olive oil, 10 g / L of glycerin, 5 g / L of tween-80, 2 g / L of yeast extract and 1 L of distilled water. 2,010 μl of the medium was placed in a 20-ml culture tube and sterilized by autoclave (121 ° C, 15 min). 1% (30 μl) of the seed culture was inoculated into the sterilized culture tube, 2% (60 μl) of the stock solution was added to each of the above Examples 1 to 12 in which DMSO was added, and the shaking incubator (35 DEG C, 200 rpm) for 36 hours. Salic acid was used as a control. 1.5 mL of the culture broth was placed in a micro tube and centrifuged at 12,000 rpm for 5 minutes using a centrifuge (Micro 17R, Hanil, Korea) to recover Malassezia. The supernatant was discarded and the concentrate was resuspended in 1.5 mL of distilled water to minimize the effect of the pigment on the natural material. The recovered Malassezia were resuspended in distilled water and absorbance was measured at 600 nm using a UV / Visible Spectrophotometer (8453 Value, Agilent, USA).

No. Relative inhibition (%) M. restricta M. globosa M. slooffiac M. sympodialis M. furfur Example 1 66 58 58 60 59 Example 2 32 7 13 -4 34 Example 3 45 54 48 52 65 Example 4 43 27 34 51 31 Example 5 -5 18 -13 23 34 Example 6 69 65 61 54 59 Example 7 62 70 69 58 57 Example 8 74 76 71 62 68 Example 9 74 75 68 65 78 Example 10 81 70 67 59 70 Example 11 90 83 79 81 84 Example 12 86 72 70 64 75 Comparative Example 1 76 52 61 58 72 Control group 86 95 96 86 91

As shown in Table 3, most of the mixed extracts of golden, yellow, white, peony and rooibus extracts using the ultrasonic extractor exhibited high antimicrobial activity. In particular, Example 11 in which 70% Respectively. The control group, salic acid, showed 86 ~ 96% antimicrobial activity. In addition, it can be confirmed that Example 11 in which rooibose is added to Comparative Example 1 is more excellent. Accordingly, it can be seen that the golden, yellow, white, peony, and rooibos mixture extracts of the present invention have antimicrobial effects against dandruff, and are effective in preventing itching and improving dandruff.

Test Example 2: Test for suppressing sebum secretion

 The 5α-reductase activity inhibition rates for the above Examples 1 to 12 were measured.

Mature Sprague-Dawley male rats (7-8 weeks old) fasted for 24 hours were sacrificed with diethyl ether, and the abdominal prostate was removed to remove connective tissue. The suspension was then finely sliced by adding buffer solution (0.32 M sucrose, 0.1 mM dithiothreitol, 20 mM sodium acetate) and suspended in a pulverizer. The suspension was centrifuged to remove supernatant Was used to partially purify 5α-reductase. Activity inhibition experiments were performed using this enzyme.

A portion of the obtained supernatant was taken into a buffer containing 0.2 M base acid and 0.2 M dibasic acid, and then reacted with a substrate (testosterone) having an isotope of ³ H to measure the amount of product dihydrootestosterone Respectively. The reaction solution was added with 1 mM dithiothreitol, 40 mM sodium phosphate (pH 6.5), 50 μM NADPH, [1,2,6,7- ³ H] testosterone / testosterone and enzyme suspension (0.5 mg) .

The sample was dissolved in 10% ethanol so as to be 1%, and 6.40 L of the sample was added to the above reaction solution so that the final concentration became 0.01%. As a control group, the same volume of solvent was used. As a positive control group, Finasteride, a substance well known as an inhibitor of 5α-reductase, approved by the US FDA, was used. Merck uses this material to manufacture and sell a product called Propecia.

The reaction was started at the same time as adding the enzyme suspension and proceeded at 37 ° C for 30 minutes and then extracted with 1 ml of ethyl acetate. 100 μl of the ethyl acetate phase was applied on a Silica plastic sheet kiesel gel F254 The developed solvent system was developed with acetate-cyclohexane (1: 1).

After drying the plastic samples in the air, the bath system was used to measure the amount of isotopes. The dried plastic sheet and x-ray film were put together in a bath cassette, and after 1 week, the testosterone and dihydrootostosterone residues Committee members were measured. The results for each inhibition rate are shown in Table 4 below.

* T: ³ H radioactivity in the testosterone area.

** DHT: ³ H radioactivity in the dihydrotestosterone region

*** Conversion rate: radioactivity to DHT area / total radioactivity

**** Inhibition rate: ((conversion rate of control group - conversion rate of sample) / conversion rate of control group) X 100

Sample (0.01%) T (dpm)) DHT (dpm) Conversion Rate (%) Inhibition rate (%) Example 1 8643 3713 30.05% 32.47% Example 2 8032 3755 31.86% 28.41% Example 3 7975 3851 32.56% 26.82% Example 4 8326 4087 32.93% 26.01% Example 5 8025 2784 25.76% 42.12% Example 6 8067 2442 23.24% 47.78% Example 7 8292 2668 24.34% 45.29% Example 8 8089 2044 20.17% 54.67% Example 9 8014 1746 17.89% 59.80% Example 10 7942 1448 15.42% 65.34% Example 11 8341 1249 13.02% 70.73% Example 12 8279 1423 14.67% 67.04% Comparative Example 1 8347 2498 19.06% 62.76% Control group 8472 6792 44.50% - Positive control group 8191 3086 27.37% 38.50%

As shown in Table 4 above, it can be confirmed that the mixed extracts rather than the single extracts are more excellent in the inhibitory effect of 5α-reductase inhibition than the hot-water extract in ultrasonic extraction (high temperature) of the functional ethanol solvent. In addition, it can be confirmed that Example 11 in which rooibose is added to Comparative Example 1 is more excellent.

Test Example 3: Pore reduction test

The hemoglobin settling rate was measured for Examples 1 to 12 above.

The pore-shrinking effect was confirmed using the bovine hemoglobin protein in Examples 1 to 12 above. A 1: 1 mixture of PBS (phosphate buffered saline, pH 7.4) and hemoglobin (1 mg / 1 ml) was prepared and centrifuged at 3000 rpm for 5 minutes. The supernatant was separated and the absorbance was measured at 407 nm . As a control, 2 ml of the mixed solvent was used instead of the sample. The pore shrinkage effect was measured by measuring the sedimentation amount of hemoglobin in the case where the final concentration of Examples 1 to 12 contained in the reaction solution was 1 to 5%. The results are shown in Table 5. The higher the sedimentation rate of hemoglobin, the better the pore shrinkage effect.

Precipitation of hemoglobin (%) Sample \ Concentration (%) 0 0.1 0.5 One 5 Control group 0.1 0.1 0.1 0.1 0.1 Example 1 0.1 1.5 4.7 8.6 25.3 Example 2 0.1 1.7 4.1 8 22.7 Example 3 0.1 1.6 3.9 7.6 23 Example 4 0.1 1.3 7.2 13.4 38.6 Example 5 0.1 2.8 10.1 24.7 66.1 Example 6 0.1 3.2 13.7 29.7 71.4 Example 7 0.1 3.8 14.9 31.4 82.8 Example 8 0.1 2.9 10.4 23.5 64.4 Example 9 0.1 3.7 14.2 30.1 77.9 Example 10 0.1 3.9 14.2 30.1 77.9 Example 11 0.1 4.2 15.6 38.8 85.4 Example 12 0.1 4.0 14.4 32.7 81.3 Comparative Example 1 0.1 3.5 13.4 28.4 74.8

As shown in the above Table 5, it can be confirmed that the mixed extract is superior to the hot water extract by the ultrasonic extraction of the ethanol solvent (high temperature) rather than the single extract, and the pore-shrinking effect is more excellent through the protein coagulation effect. In addition, it can be confirmed that Example 11 in which rooibose is added to Comparative Example 1 is more excellent.

Test Example 4: Quantitative analysis of baicalin

HPLC (Acme 9000, Youngin Instrument) assay was used to determine the baicalin content of Example 11 above. 10 mg of Baikalin (Sigma, USA) standard product was dissolved in methanol to make 100 ml, and the solution was diluted 10-fold to prepare a standard solution. In Example 11, 0.1 g was diluted 1000 times and quantitatively analyzed. The results are shown in FIG.

As shown in FIG. 1, the results of the analysis showed that the sample diluted 1,000-fold with respect to Example 11 had 2332 ppm of bacillary. Thus, it can be seen that the extract is effective against antioxidant, anti-cancer and anti-inflammatory effects of bicalin.

Test Example 5: Cytotoxicity and proliferation experiment

The cytotoxicity and proliferation experiments were carried out in the following manner. Normal human fibroblasts were inoculated into each well of a 96-well microplate to a concentration of 1 x 10 ⁴ cells and cultured in DMEM medium at 37 ° C for 24 hours. Then, the control group was changed to the DMEM medium containing 2.5% of the serum at the final concentrations of 0.1, 0.5, 1.0, 2.0 and 5.0%, and the control group was replaced with the serum-free DMEM medium for 24 hours. After incubation, MTT (Sigma, USA) solution (3 mg / ml) was added to compare the cell viability and the cell viability was measured at 570 nm using an ELISA reader (Molecular Devices, USA) The cells were cultured under the same conditions as above, and the cell proliferation rate of the test sample was calculated with the cell proliferation of the control group as 100%.

density(%) Cell proliferation (%) Control group 0.1 0.5 1.0 2.0 5.0 Example 1 100 99.8 100.0 100.3 98.9 100.1 Example 2 100 100.1 99.9 100.4 101.8 99.8 Example 3 100 100.3 101.3 101.1 100.0 99.4 Example 4 100 99.5 100.0 99.7 100.1 100.0 Example 5 100 102.8 98.8 100.2 98.6 100.9 Example 6 100 98.9 100.5 99.8 100.5 101.3 Example 7 100 100.6 101.1 100.0 100.5 100.4 Example 8 100 98.7 100.9 102.0 99.7 99.9 Example 9 100 100.1 99.7 99.3 100.2 98.7 Example 10 100 99.9 100.2 100.7 101.0 100.2 Example 11 100 100.0 97.8 100.7 99.9 99.8 Example 12 100 100.0 99.8 100.4 100.0 99.9 Comparative Example 1 100 99.9 100.0 99.9 100.2 99.8

As shown in Table 6, when the cell proliferation at the optimal cell growth condition was taken as 100%, the mixed extracts of golden, yellow, white, peony and rooibus were all cell non-toxic considering the measurement error range, .

Formulation Example 1: Preparation of hair tonic using a mixed extract of golden, yellow radish, yellowish white, peony and rooibos

1 kg of hair tonic containing the mixed extracts of golden, yellow, white, peony, and rooibos (Example 11) was prepared in the amounts shown in Table 7 below using conventional methods.

number Raw material Content (% by weight) One Golden, Yellow, White, Peony, Rooibos (Example 11) 1.0 2 ethanol 55.0 3 Castor oil 5.0 4 glycerin 3.0 5 Pyrrotonamine 0.1 6 Spices a very small amount 7 Pigment a very small amount 8 antiseptic a very small amount 9 Purified water Balance

Formulation Example 2: Hair Ampule Production Using Mixed Extract of Golden, Yellow, White, Peony, and Rooibos

1 kg of the nutrient lotion containing the mixed extracts of golden, yellow, white, peony, and rooibos (Example 11) was prepared by the conventional method in Table 8 below.

number Raw material Content (% by weight) One Golden, Yellow, White, Peony, Rooibos (Example 11) 1.0 2 ethanol 30.0 3 Propylene glycol 20.0 4 1,3-butylene glycol 10.0 5 Cetyltrimethylammonium chloride 3.0 6 Dimethyl distearyl ammonium chloride 0.1 7 Lauryl alcohol 3.0 8 Myristyl alcohol 5.0 9 Spices a very small amount 10 Pigment a very small amount 11 Tocopheryl acetate 0.1 12 Biotin 0.1 13 Purified water Balance

Test Example 5: Confirmation of formulation stability

(25 ° C.), 4 ° C. and 45 ° C. for the formulations prepared in Formulation Examples 1 and 2, which contained a mixed extract of red, gold, yellow, white, After storage for 12 weeks in a glass container, discoloration and detachment were observed with the naked eye, and the stability of these formulations was confirmed by confirming smearing by smell. The results are shown in Table 9.

Temperature
Condition Stability verification (discoloration, removal and separation) Formulation Example 1 Formulation Example 2 Room temperature 0 0 4 ℃ 0 0 45 ° C 0 0

<Formulation stability level>

0: No change, 1: Minute change, 2: Change, 3: Extreme change

As shown in Table 9, all of Formulation Examples 1 and 2 were stable at room temperature, 4 占 폚 and 45 占 폚 temperature condition without discoloration, separation and separation phenomenon.

Claims (7)

A cosmetic composition for preventing itching and improving dandruff containing a mixed extract of golden, yellow, white, peony and rooibos. [Claim 2] The cosmetic composition for preventing itching and dandruff according to claim 1, wherein the mixed extracts of gold, yellow, white, peony, and rooibos are obtained by ultrasonic extraction. [Claim 2] The cosmetic composition for preventing itching and dandruff according to claim 1, wherein the mixed extract of gold, yellow, white, peony, and rooibos is contained in an amount of 0.001 to 10 wt% based on the total weight of the cosmetic composition. The cosmetic composition for the treatment of itch and dandruff according to claim 1, wherein the mixed extract is mixed with the same weight ratio of the golden, yellow, white, peony and rooibos. The cosmetic composition according to claim 1, wherein the cosmetic composition is selected from the group consisting of hair tonic, hair conditioner, hair essence, hair lotion, hair nourishing lotion, hair shampoo, hair conditioner, hair treatment, , Hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair gel, hair mousse or hair spray. Golden, yellow, white, peony, and rooibos at a same weight ratio; At least one solvent selected from the group consisting of water in a volume of 1 to 50 times, an alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol is added and heated at 50 to 80 占 폚 1 to 2 hours; Leaving at room temperature for 7 to 10 days after filtration; And filtering and then concentrating and drying the mixture. 7. The method according to claim 6, wherein the extraction solvent is 70% by weight ethanol and the extraction temperature is 80 ° C.

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