KR101229762B1 - External Composition for promoting hair growth and preventing alopecia - Google Patents

Abstract

The present invention relates to an external preparation composition for promoting hair growth and preventing hair loss containing the complex extracts of Nasturtium herb, cypress oleander, coarse lichen, Ganoderma lucidum and half-harvest as active ingredients, and the herbal extracts are safe even when used for long-term skin. In particular, the 5 alpha-reductase (5α-reductase) inhibitory effect is excellent and can be usefully used as an external preparation for promoting hair growth and preventing hair loss.

Description

External composition for promoting hair growth and preventing hair loss {External Composition for promoting hair growth and preventing alopecia}

 The present invention relates to an external preparation composition containing a complex herbal extract as an active ingredient, specifically, a composite extract of nasturtium, baekyeop, johyeop, cypress, ganoderma lucidum and half of the extract as an active ingredient to improve hair growth and prevent hair loss. Eggplant relates to an external preparation.

The number of people who complain of hair loss due to various stresses and environmental pollution is increasing, and accordingly, the production and consumption of hair loss related products is also increasing. Hair loss refers to the increase in the number of hairs that fall out of the hair follicles of the growing or resting phase of hair follicles during the growth cycle of hair follicles. Interest is also increasing. The causes of hair loss include poor blood circulation, excessive male hormone action, excessive sebum secretion, hypodermic function caused by peroxides and bacteria, genetic factors, aging, and stress. The cause is unknown. In general, the most typical form of hair loss is androgenetic alopecia, which is caused by an excess of male hormone action, and is dependent on androgen (androgen), a male hormone. Representative androgens include testosterone and dihydrotestosterone (DHT). Patients are known to have more dihydrotestosterone (Van Neste et al., Br J Dermatol. 143 (3), pp804-810, 2000). Testosterone is converted to dihydrotestosterone by 5 alpha-reductase, and this conversion is performed by 5 alpha-reductases of Type 2 of the two types of 5 alpha-reductases, Type 1 and Type 2. It has been reported that progressive diminishing dermal papilla and hair follicles is caused by converted dihydrotestosterone (Whiting D., J Am Acad Dermatol. 28, pp755-763, 1993; Singlair R., Br Med J. 317, pp865 -886, 1998).

The most widely used in the treatment and prevention of such alopecia is the minoxidil-containing agent approved by the United States FDA, and recently, the main ingredient is propecia (finasteride) which has a 5 alpha-reductase inhibitory effect. Propecia) has been released and used. However, in the case of anti-hair loss products mainly containing chemicals such as minoxidil, there are many limitations in using them as skin side effects occur. As a result, researches for the prevention of hair loss with herbal ingredients have recently been carried out, and a topical coating hair regrowth agent prepared by mixing extracts of mulberry leaf, baekbaek leaf, black sesame, kelp, and seaweed (Korea Patent No. 10-0476791), natural situation mushroom A hair-growth composition containing the extract and a method for preparing the same (Korean Patent No. 10-0348155), a hair-growth composition consisting of an extract of Baekdusan, Daesan, and Gold having a hair follicle bactericidal function (Korean Patent No. 10-0487275) have been published. However, the clinical effects of these hair regrowths or hair growth agents are not so excellent and their mechanism of action is not clearly identified. Accordingly, research is being actively conducted to search for hair products having less skin side effects, better hair loss prevention, and hair growth promotion effects than minoxidil and propecia.

Therefore, the present inventors have experimented with various herbal medicines to overcome the above problems, and in the case of mixed extraction of nasturtium, baekryeop, johyeop, cypress, ganoderma lucidum and half of the hair growth of excellent hair growth or hair loss improvement by synergy It confirmed that there was an effect and completed this invention.

Therefore, the present invention is safe even when used in the human body for a long time by containing herbal extracts such as nasturtium, baekryeop, johyeop, cypress, Ganoderma lucidum and half-harvest complex extract as an active ingredient, providing an external preparation composition excellent in promoting hair growth and preventing hair loss. For the purpose of

According to the present invention in order to achieve the above object, it is provided with an external composition having hair growth and hair loss prevention improvement effect by containing as an active ingredient nasturtium, baekryeop, johyeop, cypress, ganoderma lucidum and half-harvest complex extract. The herbal extracts are extracted after mixing the nasturtium, baekryeop, johyeop, cypress, ganoderma lucidum and halves in a weight ratio of 1-10: 1-5: 1-5: 1-5: 1-5: 1-5 It is characterized in that, it is preferably extracted after mixing in the same weight ratio. The herbal compound extract may be prepared by extracting with one or more solvents selected from the group consisting of purified water, alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, propylene glycol and butylene glycol. The herbal compound extract is preferably contained in a ratio of 0.01 to 10% by weight, preferably 0.05 to 5% by weight, more preferably 0.1 to 2% by weight based on the total weight of the composition.

The composition for preventing and improving hair growth or hair loss is hair tonic, hair conditioner, hair essence, hair lotion, hair shampoo, hair rinse, hair treatment, hair nourishing cream, hair wax, hair pack, hair soap, hair mousse or hair spray. It can be prepared in the formulation of. In addition, the composition may be used as an external preparation for skin of the formulation, such as liquid, cream, paste or solid form.

The external preparation according to the present invention contains a complex extract of herbal medicine as an active ingredient, thereby improving the hair loss caused by promoting the secretion of androgen, a male hormone, by safe and effective inhibition of 5α-reductase activity. It is excellent in promoting hair growth and can be usefully used as an external preparation for hair.

1 is a diagram showing the hair growth effect in hair follicles using the isolated culture of rat vibrissa hair follicle of the extract of Nasturtium, Siegyeop, Chohyeop, Cypress, Ganoderma lucidum and half of the extract according to an embodiment of the present invention.
Figure 2 is a diagram showing the effect of inhibiting the 5 alpha-reductase (5α-reductase) of the complex extract of Nasturtium herb, baekryeop, cooperative, cypress, Ganoderma lucidum and half in accordance with an embodiment of the present invention.
Figure 3 is a photograph showing the hair growth effect of the composite extract of nasturtium, baekryeop, johyeop, cypress, Ganoderma lucidum and half in accordance with an embodiment of the present invention.

The present invention relates to a composition having hair growth promoting and hair loss prevention effect, containing as an active ingredient namulchocho, baekyeop, johyeop, cypress, Ganoderma lucidum and half-harvest complex extracts mixed at a specific ratio. Extracts of nasturtium, ephedra, cotyledon, cypress, ganoderma lucidum and halves, mixed in specific ratios, have synergistic effects to promote hair growth and prevent and improve hair loss.

The complex extracts of these herbs added as active ingredients in the composition of the present invention can be extracted from the roots, leaves, stems, fruits or mixtures thereof of these plants. Nasturtium (Eclipta prostrata L.) belongs to Asteraceae, is an annual herb, about 40cm high. The leaves are willow-shaped. It grows in low mountains and fields in central and southern regions. It contains ingredients such as gasoline, tannins, saponins, lactones, nicotine, ecliftin, and vitamin A. Nasturtium is used for the treatment of swelling and other wounds because it stops blood and removes pus in the private sector, and the juice has been used to blacken beard or hair.

The cypress leaves are the young branches and leaves of the Thuja orientalis L. Pharmacological action has been reported to reduce bleeding time, cough, expectorant action, and to reduce blood pressure, antibacterial action, sea water and asthma. Therefore, the baekbaekbyeol (側柏葉) is used for nosebleeds, bloody stools, urine bleeding, uterine bleeding. It is also used for seawater asthma, sputum, hair loss, seborrheic dermatitis and traumatic bleeding.

Johyup is the fruit of the legume tree (Gleditsia japonica Miquel var.koraiensis Nakai). Johyup is a name given to pods that open on trees. This drug is odorless and very sick, and when powdered it causes sneezing. The taste is spicy, warm in nature and slightly poisonous. Johyup has a strong expectorant effect and is used for pulmonary tuberculosis, lung abscess, and chronic bronchitis. It is also used for mental confusion due to a stroke, inability to greet, jeongan, and also used for boils, skin ulcers and constipation. The pharmacological effects of gastric mucosal irritation, respiratory tract endocrine secretion promotion, antibacterial action, and central nerve palsy have been reported.

Ganoderma lucidum is a mushroom of the fungus of the fungus of Democratic mushrooms. It occurs in the roots and stumps of hardwood roots in summer and also sprouts on the ground. It is a year-round mushroom with a gloss like lacquer on mushroom shade and mushroom stand. It has been known to be effective in diuresis, interpolation, tonic, mental stability, arthritis and bronchitis, and has an effect on lowering blood pressure, improving hyperlipidemia, lowering blood sugar, enhancing immune and anti-tumor effects.

Chamaecyparis obtusa is an evergreen tree of the genus Cypressaceae, also called cypress. Usually reaches 40m in height and 2m in diameter, the branches grow horizontally, the branches are drooped, the bark is reddish brown, and is torn vertically. The leaves are egg-shaped ridges, thick in quality, the fruit ripens brown in September to October, the flowers bloom in April, and the male and female run on different branches by monogamy, and the male flowers are yellow. The leaves of the baekbaek stops the hemorrhage and abdominal pain caused by gastrointestinal bleeding, and is a weak but diuretic in gonorrhea, and is used for coughing due to respiratory diseases. In the present invention, cypress is used for leaves, branches, seeds, flowers, stems or roots, preferably leaves or branches.

Pinelliae Tuber is a tuber of Pinellia ternata Breitenbach (Chunnam and Araceae), which completely removes the bark.In the muscles, essential oil, small amount of fat, starch, nicotine, low liquid, aspartic acid, glutamic acid It contains amino acids such as arginine, β-sitosterol, and choline, and has antitussive and expectorant effects. It acts as an antiviral and detoxifying agent. .

After mixing the nasturtium, ephedra, cotyledon, cypress, ganoderma lucidum and halves in a specific mixing ratio, the extraction solvent is purified water, methanol, ethanol, propanol, butanol having 1 to 4 carbon atoms, ethyl acetate, glycerin, propylene glycol and Extraction is carried out by a conventional extraction method using at least one solvent selected from the group consisting of butylene glycol. As the extraction method, extraction methods such as bath extraction, reflux cooling extraction, and ultrasonic extraction may be used.

In the preparation of the complex extract, the mixing ratio of the Nasturtium herb, baekryeop, Johyeop, Cypress, Ganoderma lucidum and halves are based on dry weight, respectively, 1-10: 1-5: 1-5: 1-5: 1-5: 1 It is mixed at a ratio of ˜5. The extract having the above mixing ratio showed excellent activity. More preferably, they are mixed in the same weight ratio. Meanwhile, the herbal extract of the present invention may be extracted after mixing the respective herbal medicines as described above, or may be mixed after obtaining the respective herbal medicine extracts.

According to one embodiment of the present invention, nasturtium leaf, baekryeop, coarse coriander, cypress, Ganoderma lucidum and half of the same dry matter or its pulverized product is added to the extraction solvent and immersed at 5-40 ° C. for 1-15 days while stirring as necessary. Or it may be extracted by heating for 4 to 20 hours at 40 ~ 100 ℃. In this case, it is preferable that the amount of the solvent used for extraction is usually 5 to 20 times the amount of the extraction raw material as a weight ratio.

Herbal complex extract according to an embodiment of the present invention may be specifically prepared according to the following examples. First, cut the nasturtium, the ephedra, cotyledon, cypress, Ganoderma lucidum, and half plants in equal proportions, and then, as an extraction solvent, purified water, ethanol, methanol, propanol, butanol, ethyl acetate, butylene glycol, propylene glycol and glycerin 5-20 times, more preferably 10-20 times at least one selected solvent is added at room temperature or a cooling condenser is installed to prevent evaporation of the solvent, stirring extraction, cold extraction, hot water extraction, ultrasonic extraction Extraction method, such as reflux cooling extraction, heating extraction, preferably stirring extraction or heating extraction extraction method, filtered and deposited for 1 to 15 days at 1 to 10 ℃. After repeating the above process 1 to 7 times, preferably 1 to 3 times, concentrated under reduced pressure and dried to obtain a herbal extract according to an embodiment of the present invention.

In the external preparation for hair growth promotion and hair loss prevention improvement of the present invention, the hair growth promotion and hair loss prevention improvement may be alleviation, prevention, or improvement of any hair loss symptom that occurs through a biological metabolic process activated by a signal factor that stimulates hair loss. However, it is preferable to alleviate, prevent or improve the alopecia caused by the secretion of androgen, a male hormone, and more specifically, to alleviate the alopecia performed by inhibiting the activity of 5α-reductase. , Prevention, or improvement.

The hair extract effect of hair follicle, dermal papilla cell proliferation effect and 5 alpha-reductase (5α-) using the mixed extract of the present invention in rat vibrissa hair follicle separation culture As a result of checking the reductase inhibitory effect, it was confirmed that the hair growth promotion and hair loss prevention effect were excellent. The mixed extract of the present invention can be directly used as an external preparation because it has the effect of promoting hair growth and hair loss prevention itself, and added to other hair growth compositions containing natural extracts effective for promoting hair growth and preventing hair loss. It is also available in the form used to extract the extracts.

The external preparations for promoting hair growth and preventing hair loss may include hair tonic, hair conditioner, hair essence, hair lotion, hair shampoo, hair rinse, hair treatment, hair nourishment cream, hair wax, hair pack, hair soap, hair mousse or hair spray. It can be prepared in the formulation of. In addition, the composition may be used as an external preparation for skin of the formulation, such as liquid, cream, paste or solid form.

The herbal complex extract of the present invention is contained in a ratio of 0.01 to 10% by weight, preferably 0.05 to 5% by weight, more preferably 0.1 to 2% by weight, based on the total weight of the external preparation composition. The composition for external preparation of the present invention is a fat, organic solvent, solubilizer, thickener and gelling agent, softener, antioxidant, suspending agent, stabilizer, foaming agent, fragrance, surfactant, water, ion in addition to the herbal extract Contains adjuvants commonly used in type or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations can do.

[Example]

Hereinafter, the present invention will be described in detail by way of the following examples and test examples, but the present invention is not limited to these examples.

Preparation Example 1 Preparation of Nasturtium Extract

1 kg of 70 kg (wt%) ethanol was purchased from Gyeongdong market, and then extracted by stirring in 10% of 70% (wt%) ethanol at room temperature for 5 days, filtered through a 250 mesh filter cloth, and left to mature at 4 ° C. for 7 days to mature. .5) was filtered to give 9.0L of an extract (91.5 g of solid content).

Preparation Example 2 Preparation of White Leaf Extract

1kg of the cypress lobe purchased from Gyeongdong market was added to 10L of 70% (wt%) ethanol, stirred and extracted at room temperature for 5 days, filtered through a 250 mesh filter cloth, left to mature at 4 ° C for 7 days, and then aged, and then Whatman filter paper (N0.5) Filtration was carried out to give 8.9 L (68.8 g of solid content).

Preparation Example 3 Preparation of Crude Extract

1kg of Johyup purchased from Gyeongdong Market was added to 10% of 70% (wt%) ethanol, stirred and extracted at room temperature for 5 days, filtered through a 250 mesh filter cloth, left to mature at 4 ° C for 7 days, and then ripened. Whatman filter paper (N0.5) The resulting mixture was filtered to give 9.2 L (solid content 81.2 g).

Preparation Example 4 Preparation of Nasturtium Herbs, Cucumber Leaf, and Cooperative Coconut Extract

500 g each of Nayeoncho, Saekyeop and Johyup purchased from Gyeongdong Market were placed in 10L of 70% (wt%) ethanol, stirred for 5 days at room temperature, filtered through a 250 mesh filter cloth, left to mature at 4 ° C for 7 days, and then aged. It filtered with (N0.5) and obtained 9.0L of extract (solid content 162.5g).

Preparation Example 5 Preparation of Nasturtium Herb, Cucumber Leaf, Johyup, Cypress, Ganoderma Lucidum Mushroom, Half Haricot Extract

500g each of Hannyeoncho, Saekyeop, Johyup, Cypress, Ganoderma lucidum, and halves purchased from Kyungdong Market were added to 10L of 70% (wt%) ethanol, stirred and extracted at room temperature for 5 days, filtered through 250 mesh filter cloth, and left at 4 ° C for 7 days. After aging, the resultant was filtered with Whatman filter paper (N0.5) to obtain 8.8 L of extract (232.1 g of solid content).

Experimental Example 1: Confirmation of hair growth effect by the isolation culture of Rat vibrissa hair follicle

In order to investigate the hair loss prevention and hair growth effect of the herbal extracts obtained in the preparation examples, the experiment was performed using the organ culture method described in the literature (Jindo and Tsuboi, Jpn. J. Dermatol., 107, pp 769-779, 1997; Imai et al., Arch.Drmatol.Res ., 284, pp466-471, 1993; Jindo et al., J. Dermatol. 20, pp756-762, 1993; Philpott et al., J. Cell Sci. , 97, pp 463-471, 1990).

Males of Wistar rat (Samtako, Korea), 3 weeks old, were anesthetized with ethyl ether and cervical spine was killed. Rat left and right mystacial pads were isolated to isolate E / P buffer solution containing 100units / ml penicillin-100µg / ml streptomycin (Gibco Inc, NY, USA) [(50% Earle ′s balanced salts). solution; EBSS, Sigma MO, USA) + (50% Phosphate-buffered saline; PBS, Sigma MO, USA). The hair follicles (vibrissa follicle) were carefully separated by observation under an anatomical microscope. Separated hair follicles in a Petri dish containing E / P buffer solution, and incubated for a while in 37 ℃, 5% CO ₂ thermostat. 2 mM L-glutamine (Gibco Inc, NY, USA), 10 μg / ml insulin (Sigma MO, USA), 50 nM hydrocortisone (Sigma MO, USA) and 100 units / ml penicillin-streptomycin per well of a 24 well plate 500 μl of William E medium (Gibco Inc, NY, USA) was added, and one hair follicle was added per well and cultured at 37 ° C. in a 5% CO ₂ incubator while changing medium every three days. The morphology of the vibrissa follicles in culture was observed under a microscope (Olympus, Japan). As a control, purified water was used, and as a positive control, minoxidil sulfate was treated at a concentration of 1 μM, and the extract obtained in Preparation Examples 1, 2, 3, 4, and 5 was treated at a concentration of 10 μM. The change in hair follicle length was photographed with an image analyzer, and the growth degree was measured by comparing with the average length of the control group, and the results are shown in FIG. 1. As confirmed in Figure 1 it can be observed that the extract of the preparation showed an improved hair growth effect compared to the control. In particular, the extracts of Nasturtium herb, cypress lobe, Johyup, Ganoderma lucidum, Cypress, and halves showed equal or better hair growth effects than minoxidil. As a result, it was confirmed that the synergistic effect was obtained by mixing the herbal medicines.

Experimental Example 2: Confirmation of the 5 alpha-reductase inhibitory effect

Based on the results of the test example, to determine whether the hair growth effect of the hair follicles by the extract treatment was due to inhibition of 5α-reductase activity, rat prostate was added using 5α-reductase [ ³ H] is converted to the amount of testosterone ^([3 H] testosterone, T) is ^[3 H] dihydro-testosterone ^([3 H] Dihydrotestosterone, DHT) were determined

Male Wistar rats 7-8 weeks of age were inhaled with ethyl ether, slaughtered the cervical spine, and removed the prostate gland and quenched with liquid nitrogen. Prostate weight was measured and 5 ml / g of 20 mM photassium phosphate buffer at pH 6.5 containing 0.32 mM sucrose (Amresco Inc, USA) and 1 mM Dithiothreitol (Sigma, USA) was added. The prostate was ground with a homogenizer and filtered by centrifugation at 1000 rpm for 10 minutes. The supernatant was recovered, centrifuged with an ultracentrifuge (Beckman, USA), and the precipitate was recovered and dissolved by adding 20 mM photassium phosphate buffer, which was used as a 5 alpha-reductase fraction. Protein concentration was quantified using a Bio-Rad protein assay kit (Bio-Rad, USA) by standardizing BSA (Bovine serum albumin). In the Eppendorf tube, the extract of the above-mentioned 5-alpha-reductase fraction 250 µg and 120 nCi [1,2,6,7-3H] -testosterone, 200 µM NADPH (Calbiochem, US), Preparation Examples 1 to 5 500 μl was added with 40 mM potassium phosphate buffer at pH 6.6. After reacting at 37 ° C. for 30 minutes, 1 ml of ethyl acetate was added and centrifuged at 1000 rpm for 10 minutes. After recovering only the supernatant and drying under reduced pressure, 50 µl of ethyl acetate was added to dissolve the solution, which was then added to a TLC (Thin layer chromatography, Merck, Germany) plate. 50:50 (ethyl acetate: cyclohexane) was used as a developing solvent. Testosterone (Testosterone) was confirmed at 254nm using a UV lamp (UVGL-58, Upland, USA), dihydrotestosterone (dihydrotestosterone (DHT)) was confirmed by heating after sprinkling 10% sulfuric acid. The testosterone and dihydrotestosterone sites were cut out of the TLC plate, placed in a scintillation vial, 5 ml of cocktail, and the cpm (counter per minute) was measured using a liquid scintilation counter (Packard Instruments, USA). . The conversion rate of testosterone by 5 alpha-reductase activity was calculated as the ratio of DHT / (testosterone + DHT) and expressed as% of the control group (FIG. 2). As shown in FIG. 2, the activity of the 5 alpha-reductase was decreased by the extract of Preparation Example. In particular, the 5 alpha-reductase activity was significantly reduced by the extract of Preparation Example 5 compared to the control.

Test Example 3: Hair Growth Effect Test

Experimental animal

Five-week-old female C57BL / 6 mice (Central Experimental Animal Co., Ltd.) were used in the experiment after adapting to the animal breeding environment for one week. Animal breeding room was maintained day and night at a temperature of 23 ± 3 ℃, 12 hours. Experimental animals were reared in mouse cages for isolation and were fed freely with experimental feed. The experimental group was set to 6 groups, and was made into a control group (sample untreated group), a positive control group (3% minoxidil (modern drug) application group), Preparation Examples 1 to 5 extract application group. Six mice in each experimental group were used for the experiment.

Sample application and observation of hair growth efficacy

Only healthy animals with no abnormalities found during quarantine and purifying periods were subjected to shaving form (Gillette foamy sensitive / Gillette (35735378) after the first epilation using electric clipper (ER143 / Panasonic / Japan) at the end of purification (P42 ~ 43day). ) / Australia) and shaving razor (Gillette Venus / Gillette (46239951) / US) for the width of the dorsal cervical to the tail proximal and at least 1 cm from the dorsal midline vertically. Second epilation was performed to select only individuals with homogeneous pink color on the dorsal skin and to use in the experiment. Group separation was performed the next day of hair removal. Groups were separated by Z-array method on the basis of weight using only animals that did not cause abrasion and severe redness during hair removal.

After brushing on the epilator site, 200 ul of test substance was dispensed on the tip of the brush using a micropipette, and then uniformly applied to the entire epilator site for 21 days once a day.

Photographs were taken using a digital camera (Canon EOS 30D / Canon / Japan) five times a day of hair removal at 0, 1, 2, 3, and 4 weeks (FIG. 3). Animals used for photography were taken by light anesthesia using dimethyl ether.

Hair growth was evaluated according to the evaluation criteria of Table 1 below.

Hair growth Scoring Judgment 0-20% - no effect 21-40% ± weak 41-60% + Good 61-80% ++ Excellent Over 81% +++ Very good

For the image files obtained from the photo evaluation, the percentage of hair growth in the total area of hair removal was calculated as a percentage (%) using Image Analysis Software (ImagePartner / SaramSoft / Korea) for each evaluation time point.

Hair regrowth rate (%) = area where hair grew / total area depilated × 100

group
hair regrowth area (%) (mean ± S.D.) 0 week 2 weeks 4 weeks Control 0 34.24 ± 15.56 85.57 ± 4.23 3% Minoxidil 0 42.78 ± 16.35 96.03 ± 2.65 Production Example 1 0 32.44 ± 7.17 77.36 ± 7.78 Production Example 2 0 23.67 ± 11.24 71.23 ± 13.24 Production Example 3 0 15.12 ± 4.98 68.76 ± 5.27 Production Example 4 0 28.45 ± 6.28 86.44 ± 4.45 Production Example 5 0 56.55 ± 14.19 94.56 ± 7.88

The hairs of the animals were taken at 7-day intervals starting from day 0 to 28 days after the end of the test. The degree of back hair growth was analyzed using Image J.

As a result, the control group that applied physiological saline only showed an average area of 34.24 ± 15.56% at 2 weeks and 85.57 ± 4.23% area at 4 weeks. Minoxidil, a positive control, showed rapid growth of hair follicles with an average area of 42.78 ± 16.35% at 2 weeks and 96.03 ± 2.65% at 4 weeks. In the case of Test Example 1, the average was increased to 32.44 ± 7.17% at 2 weeks and 77.36 ± 7.78% at 4 weeks, while in Example 2, the average was 23.67 ± 11.24% at 2 weeks and increased to 71.23 ± 13.24% at 4 weeks. . In Preparation Example 3, the average was 15.12 ± 4.98% at 2 weeks, and the average was 68.76 ± 5.27% at 4 weeks, and in Preparation Example 4, it was increased to 28.45 ± 6.28% at 2 weeks and 86.44 ± 4.45% at 4 weeks. In case of Preparation Example 5, the average increase was 56.55 ± 14.19% at 2 weeks and 94.56 ± 7.88% at 4 weeks.

On the basis of the hair growth area, the positive control group minoxidil group and Preparation Example 5 was found to be very good as (+++), Preparation Example 4 was shown to be excellent as (++), of Preparation Examples 1, 2, 3 In case there was no comparative advantage.

Example  1: Manufacture of hair tonic

Hair tonic having a composition as shown in Table 3 below was prepared according to a conventional method.

No. Raw material Content (% by weight) One Extract of Preparation Example 5 5.00 2 Castor oil 5.00 3 Resorcinol 0.10 4 menthol 0.05 5 Panthenol 0.20 6 Salicylic acid 0.10 7 Tocopheryl acetate 0.10 8 Pyridoxine hydrochloride 0.10 9 Pigment Suitable amount 10 Combination fragrance Suitable amount 11 ethanol Suitable amount 12 Purified water Balance system 100.00

Example 2: Preparation of Hair Shampoo

To prepare a hair shampoo having a composition as shown in Table 4 in accordance with a conventional method.

number Furtherance content(%) One Ammonium Lauryl Sulfate (ALS) 20.00 2 Ammonium Laures Sulfate (ALES) 20.00 3 Cocamide Mipa Suitable amount 4 Disodium iodide 0.03 5 Herbal Extract 30.00 6 Carbomer Suitable amount 7 ethanol Suitable amount 8 Extract of Preparation Example 5 5.00 9 Mixed extract 6.00 10 Cocamide Profile Betaine 6.00 11 Dimethicone (50%) 2.00 12 Methylparaben Suitable amount 13 DL-Panthenol Suitable amount 14 Tocopheryl acetate Suitable amount 15 Triethanolamine Suitable amount 16 Combination fragrance Suitable amount 17 menthol Suitable amount 18 Dimethicone (70%) 2.00 19 Methylchloroisothiazolinone Methylisothiazolinone mixture (MC / IMI) 0.03 20 Purified water Balance  system 100.00

Example 3: Hair Conditioner

A hair conditioner having a composition as shown in Table 5 below was prepared according to a conventional method.

No. Raw material content(%) One Extract of Preparation Example 5 5.00 2 Cetanol 3.50 3 Self-emulsifying glycerin monostearate 1.50 4 Propylene glycol 2.50 5 Stearylmethylbenzyl Ammonium Chloride (25%) 7.00 6 Methyl paraoxybenzoate 0.30 7 Pigment Suitable amount 8 Combination fragrance Suitable amount 9 Purified water Balance system 100.00

Example 4: Hair Lotion

To prepare a hair lotion having a composition as shown in Table 6.

No. Raw material content(%) One Extract of Preparation Example 5 5.00 2 Resorcinol 2.00 3 menthol 2.00 4 Panthenol 0.50 5 Pyrrothonol amine 0.10 6 Pigment Suitable amount 7 Combination fragrance Suitable amount 8 Purified water Balance system 100.00

Example 5: Hair Soap

To prepare a hair soap having a composition as shown in Table 7.

No. Raw material content(%) One Extract of Preparation Example 5 0.10 2 Titanium dioxide 0.20 3 Polyethylene Glycol 0.80 4 glycerin 0.50 5 Ethylenediaminetetraacetic acid 0.05 6 salt 1.00 7 Pigment Suitable amount 8 Soap Suitable amount 9 Cosmetic soap base (moisture 13%, parts by weight) Balance system 100.00

Claims (5)

External preparation composition for promoting hair growth and hair loss prevention, which contains complex extracts of Nasturtium herb, cypress lobe, johyup, cypress, Ganoderma lucidum and halves. The method of claim 1, wherein the complex extract is 1 to 10: 1-5: 1-5: 1-5: 1-5: 1-5: 1-5 with nasturtium, baekyeop, johyeop, cypress, Ganoderma lucidum and halves respectively After-mixing composition for external use characterized in that it is extracted. The method of claim 1, wherein the complex extract is prepared by extracting with at least one solvent selected from the group consisting of purified water, methanol, ethanol, propanol, butanol, ethyl acetate, glycerin, propylene glycol and butylene glycol. External composition. The composition for external preparation according to claim 1, wherein the composite extract contains 0.01 to 10% by weight based on the total weight of the external preparation. The composition according to any one of claims 1 to 4, wherein the external preparation is a hair tonic, a hair conditioner, a hair essence, a hair lotion, a hair shampoo, a hair rinse, a hair treatment, a hair nutrition cream, a hair wax, a hair pack, a hair External preparation, characterized in that the formulation is selected from the group consisting of soap, hair mousse or hair spray.

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