KR101810585B1 - Cosmetic compositions for slimming containing Fagopyrum esculentum, Ginkgo biloba and Nelumbo Nucifera Leaf complex extract, Sodium Deoxycholate and Tyrosine - Google Patents

Abstract

The present invention relates to a cosmetic composition for body slimming. More specifically, the present invention relates to a cosmetic composition for body slimming, containing 0.001-30 wt% of a composite of tyrosine, sodium deoxycholate, and an extract mixture consisting of buckwheat, ginkgo leaves, and lotus leaves with respect to the total weight of cosmetic ingredients. The cosmetic composition is safe by containing natural substances, excellently reduces cellulite, and exhibits slimming effects.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for slimming containing fragrant seeds, buckwheat, ginkgo leaf and lotus leaf blend extract, sodium deoxycholate and tyrosine complex. [0002] Cosmetic compositions for slimming containing Fagopyrum esculentum, Ginkgo biloba and Nelumbo Nucifera Leaf complex extract,

The present invention relates to a cosmetic composition for slimming, and more particularly, to a cosmetic composition for slimming, which comprises a mixture of buckwheat, ginkgo leaf and lotus leaf extract, sodium deoxycholate and a tyrosine complex to neutralize triglyceride in adipocyte Glycerol and free fatty acid, and is effective not only in reducing body fat and cellulite, but also in skin-safe cosmetic composition for slimming.

Cells that accumulate or release energy in the body are adipocytes, which are stored as triglycerides in the adipocytes when the supply is much higher than the demand for energy, and when the energy is depleted, (glycerol) and free fatty acids. Obesity is caused by the accumulation of excessive energy due to the imbalance of this process. It is caused by the increase or increase in the number of adipocytes. It usually refers to the abnormal hypertrophy of subcutaneous fat tissue below the skin layer. Cellulite is a circulatory disorder caused by excessive accumulation of fat and waste products, which causes the skin to become rugged like a rough orange peel. It occurs only in the skin of the woman and subcutaneous fat. Cellulite is not only abnormal accumulation of subcutaneous fat tissue but also lymphatic system stagnation in the body tissue gap due to abnormality of the lymphatic system, and a kind of swelling phenomenon appears at the site, and when the lymphatic fluid stagnates in the tissue gap, Not only the problem but also accumulation of toxic substances in the body adversely affect skin cells and inhibit ionization activity for nutrient intake of cells, resulting in skin necrosis as well as skin aging. Conventionally known fat decomposition promoting substances are mostly methylxanthine substances such as caffeine and theophylline, and adenylate cyclase, an enzyme closely related to lipolysis in adipocytes, (Phosphodiesterase), and the lipid-lowering effect is shown through the inhibitory effect of phosphodiesterase. However, they are known to have various adverse effects such as a widespread perception that they are addictive substances, inducing bronchodilation, or exacerbating the premenstrual syndrome phenomenon, so there is no clear guarantee for safety.

Therefore, there is a need to develop a new substance which is equivalent to or more effective than the above substances, but which is safer for the human body. The present inventors have searched natural materials showing slimming effects and found that the combination of buckwheat, ginkgo leaf and lotus leaf extract, sodium deoxycholate and tyrosine complex is effective for improving cellulite, inhibiting lipogenesis and slimming And confirmed the present invention.

Korean Patent Publication No. 2011-0136567 (December 21, 2011) Korean Patent Publication No. 2007-0025413 (2007.08.08)

Therefore, the present invention relates to a process for decomposing and discharging neutral fat accumulated in adipocytes into glycerol and free fatty acids by containing a mixture of buckwheat, ginkgo leaf and lotus leaf extract, sodium deoxycholate and tyrosine complex Which is effective for reducing obesity and reducing cellulite, has less irritation to the skin, and is safe for human body and has no side effects even when used for a long period of time. The purpose.

In order to achieve the above object, according to the present invention, there is provided a cosmetic composition for slimming comprising 0.001-30 wt% of a mixture of buckwheat, ginkgo leaf and lotus leaf extract, sodium deoxycholate and tyrosine, A composition is provided.

The complex is composed of 98 to 99.998% by weight of a mixed extract of buckwheat, ginkgo, and lotus leaf, 0.001 to 1% by weight of sodium deoxycholate and 0.001 to 1% by weight of tyrosine, 98% by weight of mixed extract of buckwheat, gingko leaf and lotus leaf, 1% by weight of sodium deoxycholate and 1% by weight of tyrosine.

The mixed extract of buckwheat, ginkgo leaf and lotus leaf can be obtained by mixing buckwheat, ginkgo leaf and lotus leaf at a weight ratio of 1: 3: 1 to 3: 1 to 3, more preferably 3: 3: 2 To be extracted.

The mixed extract is extracted with at least one extraction solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, ethyl acetate, ethylene glycol, propylene glycol, butylene glycol and glycerin, more preferably water, It is extracted by a mixed solvent of 1 to 4 alcohol.

The cosmetic composition has cellulite improvement and elasticity enhancing effect.

The cosmetic composition may be formulated into solutions, suspensions, emulsions, pastes, gels, creams, cleansing preparations containing surfactants, tonics, scaling, etc., but is not limited thereto. More specifically, it can be manufactured by a formulation such as a soft lotion, a nutritional lotion, a nutritional liquid, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water,

The combination of buckwheat, ginkgo leaf and lotus leaf blend extract, sodium deoxycholate and tyrosine according to the present invention exhibits excellent cellulite improvement and slimming effect due to its synergistic action while being safe for the human body And can be usefully used in cosmetics for slimming.

Hereinafter, the present invention will be described in detail.

Buckwheat is cultivated in various places. Height is 60 ~ 90cm and stem is empty. The roots are dullness and the root length is 90 to 120cm, which is strong against drought. Leaves are 1 to 3 nodes beneath the main stem but opposite to each other. Flowers are white and bloom in infinite inflorescence in July-October, and flowers have a lot of honey, and they become a source of honey, and they are mainly used for Taja crystal. There are 8 ~ 9 stamens and 1 pistil. Fruits are brown or dark brown when they mature, and their shape is triangular.

Buckwheat grows well on dry land, has a short growth period of 60 to 100 days, and is particularly strong in adapting to poor environments. It grows in a cool, moderate rainfall area, mild in the early stages of growth, not high temperature in the flowering stage and low in rain. However, since the growth period is short and it is adaptable to climate, it can be cultivated anywhere except the main soil to 70 ˚ N. The cultivation range is very wide. Summer buckwheat adapted to early sowing, autumn buckwheat adapted to late sowing, and intermediate type with intermediate properties. Foot is used as bare food, and leaves are used as vegetables. Seed fruit is made from buckwheat rice, which is cooked and cooked. But it is high in protein content and contains vitamin B1, B2 and nicotinic acid. Powder has become a raw material for making confectionery and cotton. In Korea, I ate mexican and cold noodles for a long time. It has a high fiber content and contains rutin, which is used as an antiparasitic or hypotensive agent. It is also grown for the purpose of producing this routine.

The bank is known as the Gongsando (孫子 木), and the shape of the leaves resembles flippers, so it is also called 脚 樹 (鴨 脚 樹). The bark is gray, thick, corky and cracked. There are two kinds of branches: long branches and short branches. Unlike most veterinary plants whose leaves are conifers, ginkgo leaves are fan-shaped and split into two at the center, but not split, and two or more split. The veins are divided into two. Leaves running on long branches cluster together in shorter branches.

The fruit grows like a ball with nucleus and ripens in yellow in October. Scalp is oval-shaped, with 2 to 3 ridges and white. The fruit is similar to the apricot, so the name of the bank is the sum of the apricot and acupuncture and the silvery silver. This seed is called white rice. The outer skin is odorous and irritates when exposed to the skin, because it has a ginkgoic acid and a mucilaginous bilobol component in the skin of the fruit.

Lotus is a perennial plant that is planted in ponds and wetlands throughout the country. It is distributed worldwide in East Asia, South Asia, Australia, North America and so on. The rootstock is thick, has many holes in it, and stretches sideways from the ground. Beard roots and leaves come from nodes of rootstock. Leaves are round shield, 30 ~ 90cm in diameter, concave in the middle, with flat edges. Petioles are 1 ~ 2m long. The flowers hang one by one at the end of a flower that rises above water in July ~ August, and it is red, pink, white, and 10 ~ 25cm in diameter. It keeps pumping for only 3-4 days in the daytime. Calyx is 4-5, green, early fall. Petals are 16 ~ 24, obovate. There are about 400 operations, and the anther is yellow. Bracts are spongy, pointy, and flat on the surface. The fruit resembles a nut and runs one by one in a hole in a bush, oval, and black. It is cultivated for ornamental use, and it is commonly used for edible purposes and cultivated to obtain roots. It is used to make petals and leaves by tea, and to use steamed leaves to make steamed dishes.

Sodium Deoxycholate is a sodium salt of deoxycholic acid. Deoxycholic acid is one of the bile acids, which makes various organic compounds and molecular compounds (colic acid). The sodium salt acts as a surface active agent and acts to destroy any type of bacteria or intracellular granule structure.

Tyrosine (Tyrosine) is an essential amino acid for natural melanin production. One of the aromatic α-amino acids is a glossy, minute needle-shaped crystal with a molecular weight of 191.19 and a melting point of 314 to 318 ° C. It is a non-essential amino acid and does not dissolve well in water. Protein xanthoprotein (yellow) and milone (red) reactions are caused by tyrosine and detected and quantified by these reactions. It is contained in most proteins, but it is mostly caused by casein and silk fibroin, and is caused by decomposition by hydrolysis, digestion and decay thereof. It is also found in old cheese and also in glass form. In 1846 Liebig was found in the alkaline degradation products of casein, and Erlenmeyer succeeded in synthesizing the structure. Di (di) - and mono (iodo) tyrosine are present in the thyroid with thyroxine, the main thyroid hormone, and iodine tyrosine also exists in various seaweeds and sponges. Tyrosine is produced in vivo by enzymatic hydrolysis of phenylalanine. There are two pathways for oxidative degradation of tyrosine. One is the pathway for the degradation of fumaric acid and acetoacetic acid. The other is the pathway for the production of melanin, adrenal hormone, and adrenaline by the change of 3, 4-dioxyphenylalanine by tyrosinase. Congenital metabolic abnormalities are greatly contributing to the metabolism studies of tyrosine. These include arctapenuria, phenylketonuria, or pigment deficiency, all of which are thought to be caused by a lack of a metabolic pathway.

The present invention utilizes the fact that, when a mixture of sodium deoxycholate and tyrosine is mixed with buckwheat, ginkgo leaf and lotus leaf blend extract, the cellulite improvement and the slimming effect are enhanced to be more excellent .

According to the present invention, there is provided a cosmetic composition for slimming comprising 0.001-30% by weight of a mixture of buckwheat, ginkgo leaf and lotus leaf mixture, sodium deoxycholate and tyrosine, based on the total weight of the cosmetic.

The complex is composed of 98 to 99.998% by weight of a mixed extract of buckwheat, ginkgo, and lotus leaf, 0.001 to 1% by weight of sodium deoxycholate and 0.001 to 1% by weight of tyrosine, 98% by weight of mixed extract of buckwheat, gingko leaf and lotus leaf, 1% by weight of sodium deoxycholate and 1% by weight of tyrosine.

The mixed extracts of buckwheat, ginkgo, and lotus leaves are mixed at a weight ratio of 1: 3: 1 to 3: 1 to 3, respectively, and mixed at a ratio of 3: 3: 2. The yield and the slimming effect are most excellent.

The mixed extract is extracted with at least one extraction solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, ethyl acetate, ethylene glycol, propylene glycol, butylene glycol and glycerin, more preferably water, It is extracted by a mixed solvent of 1 to 4 alcohol.

According to one embodiment of the present invention, the mixed extract of buckwheat, ginkgo leaf and lotus leaf can be prepared by the following method.

First, buckwheat, ginkgo leaf, and dried lotus leaves are cut off, and an extraction solvent selected from water, 1 to 4 carbon atoms, or alcohols having a carbon number of 1 to 4 or a hydrated alcohol thereof is added at a rate of 1 to 30 For 5 days, extracting the active ingredient, and concentrating the extraction solvent with a vacuum concentrator.

According to another example, buckwheat, gingko leaf and dried lotus leaf blades are pulverized and mixed, and they are mixed with water in a volume of 1 to 30 times the dry weight thereof, alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene Glycol, or butylene glycol, or a mixture thereof as a solvent, the solvent is extracted by heating with an extractor equipped with a cooling condenser at 30 to 130 ° C for 1 to 48 hours to prevent evaporation of the solvent, Immersing it for 5 days, extracting the active ingredient, and concentrating the extracting solvent with a vacuum concentrator. Further, it can be produced by using an ultrasonic extraction method known in the above-mentioned extraction solvent.

Sodium deoxycholate and tyrosine are mixed with the mixed extract of buckwheat, ginkgo leaf and lotus leaf thus prepared to prepare a complex. The above-mentioned mixed extracts of buckwheat, ginkgo, and lotus leaves showed excellent slimming effects. However, when sodium deoxycholate and tyrosine were mixed, they showed better cellulite improvement and slimming effect .

In this case, the complex is composed of 98 to 99.998% by weight of a mixed extract of buckwheat, ginkgo leaf and lotus leaf, 0.001 to 1% by weight of sodium deoxycholate and 0.001 to 1% by weight of tyrosine, 98% by weight of mixed extract of buckwheat, ginkgo leaf and lotus leaf, 1% by weight of sodium deoxycholate and 1% by weight of tyrosine.

The composite thus prepared may be added to the cosmetic in a proportion of 0.001 to 30% by weight, more preferably 0.001 to 5.0% by weight, based on the total weight of the cosmetic.

The complex thus prepared promotes the decomposition of triglyceride into glycerol and free fatty acid in adipocytes, and has effects of reducing body fat and cellulite, and also has a skin-safe body slimming effect Respectively.

The cosmetic composition of the present invention is not particularly limited in its formulation and can be applied to cosmetic compositions such as external liquid, cream, ointment, paste, aerosol, gel, wax and the like. In addition, in the cosmetic composition of each formulation, components other than the above-mentioned compound extracts of Gugija, green tea, chrysanthemum, yulmu, ginger, omija, persimmon leaves, dandelion, Can be arbitrarily selected and blended. For example, it may include colorants (coloring matters), flavoring agents (flavoring agents), suspending agents, emulsifying agents, dissolution aids, stabilizers, isotonic agents, pH adjusting agents, viscosity adjusting agents, solvents, preservatives and the like which are conventionally used in cosmetic compositions .

In addition, the cosmetic composition of the present invention may further comprise other active ingredients, in addition to the above-mentioned complex, in which the cellulite improvement and slimming cosmetic composition are effective.

[Example]

Hereinafter, the constitution and effects of the present invention will be described in detail by way of examples and the like, but the present invention is not limited thereto.

Production Example 1: Production of mixed extract of buckwheat, ginkgo leaf and lotus leaf

300 g, 300 g and 200 g of buckwheat, ginkgo leaf and lotus leaf were respectively washed with purified water, dried and used. 2 kg of 70% ethanol was added and the mixture was boiled for 4 hours at 60 ~ 80 ℃ in an extractor equipped with a condenser.

The filtrate was subjected to primary filtration with a paper filter paper, followed by secondary filtration with a membrane filter having a pore size of 1.0 mu m, followed by final filtration with a membrane filter having a pore size of 0.45 mu m. The mixture was concentrated at a temperature of 40 to 50 ° C using a vacuum concentrator (product name: Coolace CCA-1100, manufactured by EYELA), and then dried using a drier (model name: B-290, manufactured by BUCHI) Was obtained.

Production Examples 2 to 22 Preparation of mixed extract of buckwheat, gingko leaf and lotus leaf with the extraction ratio

The weight ratio of buckwheat, ginkgo leaf and lotus leaf was adjusted as shown in Table 1 (total weight: 800 g), and extraction and filtration were carried out in the same manner as in Preparation Example 1 above.

division Mixing ratio Production Example 1 3: 3: 2 Production Example 2 1: 1: 1 Production Example 3 2: 1: 1 Production Example 4 1: 2: 1 Production Example 5 1: 1: 2 Production Example 6 3: 1: 1 Production Example 7 1: 3: 1 Production Example 8 1: 1: 3 Production Example 9 1: 2: 3 Production Example 10 1: 3: 2 Production Example 11 2: 1: 2 Production Example 12 2: 1: 3 Production Example 13 2: 2: 1 Production Example 14 2: 3: 1 Production Example 15 2: 2: 3 Production Example 16 2: 3: 2 Production Example 17 2: 3: 3 Production Example 18 3: 1: 2 Production Example 19 3: 2: 1 Production example 20 3: 2: 2 Production Example 21 3: 3: 1 Production Example 22 3: 2: 3

Comparative Preparation Example 1: Preparation of buckwheat seed extract

800 g of buckwheat, purchased from Kyungdong market, was washed with purified water and dried, and the same procedure as in Preparation Example 1 was carried out.

Comparative Preparation Example 2: Preparation of Ginkgo biloba extract

800 g of ginkgo biloba purchased from Kyungdong market was washed with purified water and dried, and then the same procedure as in Production Example 1 was carried out.

Comparative Preparation Example 3: Preparation of lotus leaf extract

800 g of lotus leaf purchased from Kyungdong market was washed with purified water and dried, and then the same procedure as in Production Example 1 was carried out.

Test Example 1: Identification of cell non-toxicity and cell proliferation effect

The cell proliferation effect of the buckwheat, ginkgo leaf and lotus leaf blend extracts prepared in the above Preparation Example was measured using cell culture. Sodium Deoxycholate and Tyrosine were purchased and used in Comparative Preparation Examples 4 and 5.

1) Experimental method

The cytotoxicity and proliferation experiments were carried out in the following manner. Cultured human normal fibroblasts were inoculated into each well of a 96-well microplate to a concentration of 1 × 10 ⁴ cells and cultured in DMEM medium at 37 ° C. for 24 hours. Then, the control group was changed to the DMEM medium containing 2.5% of the serum at the final concentrations of 0.1, 0.5, 1.0, 2.0 and 5.0%, and the control group was replaced with the serum-free DMEM medium for 24 hours. After incubation, MTT (Sigma, USA) solution (3 mg / ml) was added to compare the cell viability and the cell viability was measured at 570 nm using an ELISA reader (Molecular Devices, USA) The cells were cultured under the same conditions as above, and the cell proliferation rate of the test sample was calculated with the cell proliferation of the control group as 100%.

2) Experimental results

The cell proliferation rate was calculated by the following equation, and the results are shown in Table 2 below.

density(%) Cell proliferation (%) Average Control group 0.1 0.5 1.0 2.0 5.0 Production Example 1 100 102.9 105.6 106.5 103.5 102.2 104.14 Production Example 2 100 103.7 102.9 101.3 100.3 100 101.64 Production Example 3 100 105.8 103.9 102.1 103.7 101 103.3 Production Example 4 100 101.6 101.8 100.9 100.4 99.6 100.86 Production Example 5 100 101.7 100.4 99.7 101 99.6 100.48 Production Example 6 100 100.9 101.5 100.8 99.9 98.9 100.4 Production Example 7 100 101.7 100.4 99.7 101 99.6 100.48 Production Example 8 100 100.9 101.5 100.8 99.9 98.9 100.4 Production Example 9 100 101.2 100.7 100.5 100.8 97.8 100.2 Production Example 10 100 102.9 101.4 100.1 99.5 99.8 100.74 Production Example 11 100 101.3 100.4 99.7 101 99.6 100.4 Production Example 12 100 105.8 101.0 99.4 99.6 99.9 101.14 Production Example 13 100 103.3 103.6 99.1 100.2 97.2 100.68 Production Example 14 100 101.8 101.2 100.8 96.8 100.5 100.22 Production Example 15 100 102.6 102.4 98.2 100.0 101.1 100.86 Production Example 16 100 101.3 101.1 100.9 99.6 99.4 100.46 Production Example 17 100 100.8 100.6 101.6 98.2 100.7 100.38 Production Example 18 100 99.3 99.2 100.3 102.8 101.0 100.52 Production Example 19 100 105.8 99.8 98.9 101.5 99.3 101.06 Production example 20 100 104.3 103.4 98.9 92.4 102.6 100.32 Production Example 21 100 98.8 101.2 101.4 101.5 100.9 100.76 Production Example 22 100 103.3 100.6 99.1 100.5 99.2 100.54 Comparative Preparation Example 1 100 101.5 101.8 100.9 100.1 98.6 100.58 Comparative Production Example 2 100 103.5 99.9 101.3 100.3 100.01 101.00 Comparative Production Example 3 100 100.7 98.6 100.8 99.9 98.9 99.78 Comparative Production Example 4 100 101.3 101.9 100.9 100.2 99.6 100.78 Comparative Preparation Example 5 100 101.4 100.1 99.7 101.1 99.6 100.38

As shown in Table 2, when the cell proliferation at the optimal cell growth conditions was taken as 100%, the mixed extracts of buckwheat, ginkgo leaf, and lotus leaf were all cell non-toxic and had cell proliferation effects considering the measurement error range .

Test Example 2: First skin irritation test

To examine the skin irritation of the mixed extracts of buckwheat, ginkgo leaf and lotus leaf preparations of Preparation Examples 1 to 22, 10% (wt / v) of each sample was applied to the back region of a healthy adult male and female ) After spraying a certain amount (0.2 g) of purified water into the purified water, the pin chamber was removed and skin condition after 4 hours was judged by the following criteria and the degree of stimulus.

<Criteria>

-: No erythema or unusual phenomenon

±: slightly redder than around

+: Significantly reddened than surrounding

++: more reddened and bulging than ambient

sample Number of test subjects (persons) Judgment result Irritation degree ++ + ± - Production Example 1 25 - - - 25 0.00 Production Example 2 25 - - - 25 0.00 Production Example 3 25 - - - 25 0.00 Production Example 4 25 - - - 25 0.00 Production Example 5 25 - - One 24 0.01 Production Example 6 25 - - - 25 0.00 Production Example 7 25 - - - 25 0.00 Production Example 8 25 - - - 25 0.00 Production Example 9 25 - One - 24 0.01 Production Example 10 25 - - - 25 0.00 Production Example 11 25 - - - 25 0.00 Production Example 12 25 - One - 24 0.01 Production Example 13 25 - - One 24 0.01 Production Example 14 25 - - - 25 0.00 Production Example 15 25 - - - 25 0.00 Production Example 16 25 - - - 25 0.00 Production Example 17 25 - - - 25 0.00 Production Example 18 25 - - One 24 0.01 Production Example 19 25 - One - 24 0.01 Production example 20 25 - - - 25 0.00 Production Example 21 25 - - - 25 0.00 Production Example 22 25 - - - 25 0.00 Comparative Preparation Example 1 25 - - One 24 0.01 Comparative Production Example 2 25 - - - 25 0.00 Comparative Production Example 3 25 - - - 25 0.00 Comparative Production Example 4 25 - One - 24 0.01 Comparative Preparation Example 5 25 - - - 25 0.00

As can be seen from Table 3, the extracts of buckwheat, gingko leaf and lotus leaf extract according to the present invention had no skin irritation.

Test Example 3: Measurement of inhibition rate of adipocyte differentiation

The following experiments were conducted to examine the inhibitory effect of the extracts of the above Preparation Examples and Comparative Preparations on the differentiation of 3T3-L1 cell line (Korean Cell Line Bank, KCLB 10092.1) into undifferentiated adipocytes.

The undifferentiated 3T3-L1 cell line was maintained in DMEM medium containing 10% bovine calf serum. The undifferentiated 3T3-L1 cell line was cultured to a density of 90%, and then DMEM medium containing 10% fetal bovine serum and dexamethasone, IBMAX, and insulin was changed to induce differentiation. The extracts of the comparative preparation examples and the preparation examples were dissolved in dimethyl sulfoxide (DMSO) and treated continuously. After incubation for 2 days, the medium was replaced with DMEM medium containing 10% fetal bovine serum and insulin. Subsequently, DMEM media containing only 10% fetal calf serum was replaced at two-day intervals. After 8 days from the induction of regeneration, regeneration into the local taxa was completed. At this time, the control group was replaced with the same amount of DMSO at the same interval as the experimental group. After the regeneration is completed, the oil red ocher is extracted from the cells, and then the absorbance (500 nm) of the solution is measured with a spectrophotometer to determine the differentiation into adipocytes Respectively. The inhibition rate of adipocyte differentiation was calculated by the following equation.

The results are shown in Table 4 below.

division Differentiation inhibition rate (%) Production Example 1 One% 31.12 Production Example 2 One% 20.60 Production Example 3 One% 21.12 Production Example 4 One% 20.15 Production Example 5 One% 19.64 Production Example 6 One% 23.82 Production Example 7 One% 20.51 Production Example 8 One% 22.50 Production Example 9 One% 23.16 Production Example 10 One% 22.51 Production Example 11 One% 21.54 Production Example 12 One% 23.32 Production Example 13 One% 23.60 Production Example 14 One% 23.12 Production Example 15 One% 23.64 Production Example 16 One% 23.82 Production Example 17 One% 24.51 Production Example 18 One% 26.50 Production Example 19 One% 25.16 Production example 20 One% 27.51 Production Example 21 One% 26.54 Production Example 22 One% 28.32 Comparative Preparation Example 1 One% 15.94 Comparative Production Example 2 One% 14.70 Comparative Production Example 3 One% 16.40 Comparative Production Example 4 One% 15.94 Comparative Preparation Example 5 One% 16.70

As shown in Table 4, the extracts of buckwheat, ginkgo leaf and lotus leaf blend according to the present invention exhibited higher adipocyte differentiation inhibitory activity than the respective extracts due to the synergistic effect, and showed the highest activity in Preparation Example 1 . Thus, it can be seen that the buckwheat seeds, ginkgo leaf, and lotus leaf blended extracts of the above-mentioned production examples have excellent slimming effects.

Examples 1 to 4: Preparation of buckwheat, ginkgo leaf and lotus leaf mixed extract, sodium deoxycholate and tyrosine complex

Sodium deoxycholate and tyrosine were mixed with the mixed extract of Preparation Example 1 showing the most excellent fat-decomposing effect in the proportions shown in Table 5 below to prepare a composite.

(weight%) Example Comparative Example One 2 3 4 One 2 3 4 5 Buckwheat, ginkgo leaf and lotus leaf extract 98 99.8 99.8 99.8 100 99 99 99.9 99.9 Sodium deoxycholate One 0.15 0.05 0.1 - One - 0.1 - Tyrosine One 0.05 0.15 0.1 - - One - 0.1

Formulation Examples 1 to 4: Manufacture of Massage Cream

1 kg of a massaging cream containing the above complex was prepared using the following preparation method in the contents shown in Table 6 below.

number Raw material Content (% by weight) Formulation Example Comparative Formulation Example One 2 3 4 One 2 3 4 5 One Complex (Example 1) 3.0 - - - - - - - - Complex (Example 2) - 3.0 - - - - - - - Complex (Example 3) - - 3.0 - - - - - - Complex (Example 4) - - - 3.0 - - - - - Composite (Comparative Example 1) - - - - 3.0 - - - - The composite (Comparative Example 2) - - - - - 3.0 - - - Compound (Comparative Example 3) - - - - - - 3.0 - - Composite (Comparative Example 4) - - - - - - - 3.0 - The composite (Comparative Example 5) - - - - - - - - 3.0 2 Carboxyvinyl polymer 0.45 0.45 0.45 0.45 0.45 0.45 0.45 0.45 0.45 3 Triethanolamine 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 0.9 4 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 5 Cetyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 6 Figure-30
Glyceryl cocoate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 7 PHY-80
Glyceryl cocoate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 8 Sodium coco amphopropionate 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 9 Butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 10 Phenoxyethanol a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount 11 Spices a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount 12 Pigment a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount 13 Purified water Balance Balance Balance Balance Balance Balance Balance Balance Balance

In Table 6, substances 9, 10, and 13 were heated to 80 to 85 ° C. while being mixed and stirred. Then, the mixture was put into a manufacturing part, and then an emulsifier was allowed to react. Substances 2, 3, 4, 6, And then emulsified. After the emulsification is completed, the mixture is cooled to 50 ° C with stirring using an agitator, and then the substance 11 is added. After cooling to 45 ° C, the substance 12 is added. When the emulsification is completed, the mixture is cooled to 35 ° C with stirring using a stirrer, The mixture was cooled to 25 캜 and aged at 25 캜 for 3 days to prepare a head massage cream.

Test Example 4: Confirmation of formulation stability

The formulations prepared in Formulation Examples 1 to 4 were stored in an opaque glass container at room temperature (25 ° C), 4 ° C and 45 ° C in a room, a refrigerator and a thermostatic water bath for 12 weeks, And separation were observed, and the stability of these formulations was confirmed by confirming smearing by smell. The results are shown in Table 7.

Temperature condition Stability verification (discoloration, removal and separation) Formulation Example Comparative Formulation Example One 2 3 4 One 2 3 4 5 Room temperature 0 0 0 0 0 0 0 0 0 4 ℃ 0 0 0 0 0 0 0 0 0 45 ° C 0 0 0 0 0 0 0 0 0

<Formulation stability level>

0: No change, 1: Minute change, 2: Change, 3: Extreme change

All of Formulation Examples 1 to 4 were stable at room temperature, 4 占 폚 and 45 占 폚 temperature condition without discoloration, separation and separation phenomenon.

Test Example 5: Slimming Effect Clinical Test Evaluation

Slimming effect on face

10 women each aged 30 to 60 years or older who feel that the normal face area swells well are allowed to use the massage cream of Formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 5 on the face for 2 weeks. The slimming effect was evaluated by measuring the facial volume of PRIMOS Body before use, immediately after use, and 2 weeks after use, and calculating the difference in facial area volume. The results are shown in Tables 8 to 17 below, respectively.

Subject number Formulation Example 1 Before use (㎣) After 2 weeks of use (㎣) Increase / decrease (㎣) One 213.17 198.37 -14.80 2 151.27 148.47 -2.80 3 223.27 224.09 +0.82 4 172.13 170.53 -1.60 5 162.39 161.98 -0.41 6 131.39 130.11 -1.28 7 160.45 146.39 -14.06 8 124.31 115.29 -9.02 9 176.37 174.09 -2.28 10 139.79 136.94 -2.85 Average 165.45 160.62 -4.82 Standard Deviation 32.54 32.71 0.17

Subject number Formulation Example 2 Before use (㎣) After 2 weeks of use (㎣) Increase / decrease (㎣) One 125.31 117.41 -7.9 2 144.22 148.13 +3.91 3 119.74 111.06 -8.68 4 171.34 164 -7.34 5 213.47 204.82 -8.65 6 245.55 243.61 -1.94 7 214.19 205 -9.19 8 175.93 184.09 +8.16 9 270.3 257.34 -12.96 10 170.62 171.73 +1.11 Average 185.06 180.71 -4.34 Standard Deviation 50.07 48.63 1.43

Subject number Formulation Example 3 Before use (㎣) After 2 weeks of use (㎣) Increase / decrease (㎣) One 140.17 139.11 -1.06 2 144.21 140.13 -4.08 3 119.73 111.04 -8.69 4 155.54 154.18 -1.36 5 178.91 175.37 -3.54 6 125.95 123.48 -2.47 7 144.27 130.14 -14.13 8 119.25 112.2 -7.05 9 126.3 125.53 -0.77 10 151.41 152.39 +0.98 Average 140.57 136.35 -4.21 Standard Deviation 18.69 20.17 1.48

Subject number Formulation Example 4 Before use (㎣) After 2 weeks of use (㎣) Increase / decrease (㎣) One 120.26 109.3 -10.96 2 89.58 87.92 -1.66 3 120.41 121.86 +1.45 4 171.28 166.76 -4.52 5 168.23 163.64 -4.59 6 171.13 166.57 -4.56 7 169.66 173.73 +4.07 8 245.16 241.11 -4.05 9 213.34 203.19 -10.15 10 166.99 162.85 -4.14 Average 163.604 159.693 -3.91 Standard Deviation 45.38 44.675 0.70

Subject number Comparative Formulation Example 1 Before use (㎣) After 2 weeks of use (㎣) Increase / decrease (㎣) One 142.19 141.07 -1.12 2 121.78 119.32 -2.46 3 141.87 137.09 -4.78 4 155.56 155.16 -0.40 5 175.78 173.15 -2.63 6 125.93 121.48 -4.45 7 168.34 165.49 -2.85 8 184.35 181.92 -2.43 9 178.93 184.09 +5.16 10 270.3 268.34 -1.96 Average 166.503 164.711 -1.79 Standard Deviation 42.55 43.23 0.67

Subject number Comparative Formulation Example 2 Before use (㎣) After 2 weeks of use (㎣) Increase / decrease (㎣) One 171.13 165.57 -5.56 2 169.66 172.73 +3.07 3 135.87 134.01 -1.86 4 155.56 154.16 -1.40 5 178.90 174.35 -4.55 6 125.57 118.37 -7.20 7 146.34 144.49 -1.85 8 134.34 120.91 -13.43 9 121.93 119.53 -2.40 10 151.46 151.55 +0.09 Average 149.07 145.56 -3.50 Standard Deviation 19.82 21.69 1.87

Subject number Comparative Formulation Example 3 Before use (㎣) After 2 weeks of use (㎣) Increase / decrease (㎣) One 140.19 139.07 -1.12 2 121.78 119.32 -2.46 3 140.87 139.09 -1.78 4 171.13 166.57 -4.56 5 169.66 159.73 -9.93 6 124.93 124.18 -0.75 7 166.34 165.49 -0.85 8 184.65 171.97 -12.68 9 126.93 125.53 -1.40 10 151.31 152.39 +1.08 Average 149.779 146.334 -3.44 Standard Deviation 22.20 19.46 2.74

Subject number Comparative Formulation Example 4 Before use (㎣) After 2 weeks of use (㎣) Increase / decrease (㎣) One 175.13 171.09 -4.04 2 270.3 265.34 -4.96 3 140.85 138.09 -2.76 4 153.56 144.16 -9.40 5 178.9 173.35 -5.55 6 125.83 123.38 -2.45 7 167.34 165.49 -1.85 8 184.35 180.92 -3.43 9 124.93 126.53 +1.60 10 151.15 151.25 +0.10 Average 167.234 163.96 -3.20 Standard Deviation 41.84 40.79 1.05

Subject number Comparative Formulation Example 5 Before use (㎣) After 2 weeks of use (㎣) Increase / decrease (㎣) One 140.19 129.07 -11.12 2 121.78 118.32 -3.46 3 140.77 138.88 -1.89 4 155.51 154.16 -1.35 5 175.9 174.35 -1.55 6 125.23 124.48 -0.75 7 136.34 133.45 -2.89 8 154.35 149.92 -4.43 9 171.13 169.57 -1.56 10 169.66 165.73 -3.93 Average 149.086 145.793 -3.29 Standard Deviation 19.21 19.87 0.65

Formulation Example Comparative Formulation Example One 2 3 4 One 2 3 4 5 Average -4.82 -4.34 -4.21 -3.91 -1.79 -3.50 -3.44 -3.27 -3.29

There was a variation in each subject, but in the case of using the massage cream of Formulation Examples 1 to 4, the volume of the facial region decreased more than that of Comparative Formulations 1 to 5. Among them, it was confirmed that the use of the massage cream of Formulation Example 1 shows the best reduction of the volume of the face region.

Slimming effect on thigh

90 women with a body mass index (BMI) of 21-27 were randomly divided into nine groups of 10 women, each of which had a body mass index (BMI) of 21-27 years old. The massage cream of Formulation Examples 1 to 6 was used. Five of 10 patients in each group applied the massage cream of the above Formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 5 to the left thigh and 5 patients to the right thigh. After 8 weeks of using the massage cream, the circumference of the thigh before and after use was measured using a tape measure (mm), and the slimming effect was evaluated by calculating the difference in thigh circumference. The difference in the length of the thigh circumference is shown in Table 18 below.

Formulation Example Comparative Formulation Example One 2 3 4 One 2 3 4 5 Average length change 35mm 29mm 28mm 25mm 11mm 21mm 22mm 15mm 17mm

As can be seen in Table 18 above, it can be seen that the change in length around the thigh of the evaluator population using the massage cream of the formulation embodiment is much greater than the change in the length of the population using the massage cream of Comparative Formulations 1 to 5.

Slimming effect on abdomen

For women with localized obesity or cellulite, 90 adult women aged 20 to 50 years with a Body Mass Index (BMI) of 21-27 were randomly assigned to one or two daily doses in the morning and evening for 10 minutes, Massage cream of comparative formulation example was massaged. After 5 weeks, abdominal fat percentage and body fat percentage before and after application were evaluated using a body composition meter. Abdominal fat percentage was calculated by dividing the maximum waist circumference measured on the umbilical line by the hip circumference length. The results are shown in Table 19 below.

Before Control After application of formulation increase Formulation Example 1 % Body fat 29.2 24.7 -4.5 Abdominal fat rate 0.86 0.51 -0.35 Formulation Example 2 % Body fat 27.8 24.3 -3.5 Abdominal fat rate 0.8 0.59 -0.21 Formulation Example 3 % Body fat 28.9 25.6 -3.3 Abdominal fat rate 0.84 0.59 -0.25 Formulation Example 4 % Body fat 28.4 25.6 -2.8 Abdominal fat rate 0.74 0.55 -0.19 Comparative Formulation Example 1 % Body fat 30.1 28.89 -1.21 Abdominal fat rate 0.75 0.72 -0.03 Comparative Formulation Example 2 % Body fat 31.1 28.6 -2.5 Abdominal fat rate 0.75 0.64 -0.11 Comparative Formulation Example 3 % Body fat 26.7 24.4 -2.3 Abdominal fat rate 0.83 0.74 -0.09 Comparative Formulation Example 4 % Body fat 28.6 26.62 -1.98 Abdominal fat rate 0.79 0.72 -0.07 Comparative Formulation Example 5 % Body fat 28.6 26.71 -1.89 Abdominal fat rate 0.77 0.71 -0.06

As can be seen from the above Table 19, when the massage cream of the formulation example containing the buckwheat seeds, ginkgo leaf and lotus leaf mixture extract, sodium deoxycholate and tyrosine complex of the present invention is used, And abdominal fat rate decreased significantly.

Skin elasticity enhancement effect

The thigh slimming effect test participants were asked to evaluate the elasticity enhancement effect. The degree of elasticity enhancement was classified into five grades as shown below, and the average value results thereof are shown in Table 20 below.

-2: Very decrease in elasticity -1: Moderate decrease in elasticity 0: No change in elasticity

1: slightly increased elasticity 2: very good elasticity

Formulation Example Comparative Formulation Example One 2 3 4 One 2 3 4 5 Degree of elasticity enhancement 2 One One 0 0 0 0 0 0

From the results shown in Table 20, it can be seen that the massage cream of the formulation example also has an elasticity-enhancing effect.

Claims (6)

Ginkgo seeds, ginkgo leaf and lotus leaf blend extract, sodium deoxycholate and tyrosine complex, which are extracted by mixing buckwheat, gingko leaf and lotus leaf at a weight ratio of 1: 3: 1 to 3: By weight based on the total weight of the cosmetic composition. The composition according to claim 1, wherein the complex comprises 98 to 99.998% by weight of a mixture of buckwheat, ginkgo leaf and lotus leaf extract, 0.001 to 1% by weight of sodium deoxycholate and 0.001 to 1% by weight of tyrosine Wherein the cosmetic composition is a cosmetic composition for slimming. delete The method according to claim 1, wherein the mixed extract is extracted with at least one extraction solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, ethyl acetate, ethylene glycol, propylene glycol, butylene glycol and glycerin A cosmetic composition for slimming. The cosmetic composition for slimming according to claim 1, wherein the cosmetic composition has cellulite improving effect. The cosmetic composition for slimming according to claim 1, wherein the cosmetic composition has an elasticity-enhancing effect.