KR101677466B1 - Anti-wrinkle Cosmetic Composition Comprising Hylocereus undatus (Haw.) Britt. and Rose Extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving wrinkles containing an extract and an extract as an active ingredient. More particularly, the present invention relates to a cosmetic composition having excellent antioxidative, anti-aging and anti-wrinkle effects by removing harmful active oxygen and protecting skin cells, and keeping the skin elastic and bright by containing the extract and the extract as an active ingredient in a cosmetic base . The dragon and extract of the present invention has an antioxidative effect by removing active oxygen in the skin and protecting the skin from cell damaging substances and promoting collagen biosynthesis by promoting the proliferation of skin cells, It is possible to prepare a cosmetic composition which exhibits excellent antioxidation, anti-aging and wrinkle-reducing effects by containing the above-mentioned extract and the extract as an active ingredient in a cosmetic base.

Description

(Anti-wrinkle Cosmetic Composition Comprising Hylocereus undatus (Haw.) Britt. and Rose Extract}

The present invention relates to a cosmetic composition for improving wrinkles containing an extract and an extract as an active ingredient. More particularly, the present invention relates to a cosmetic composition having excellent antioxidative, anti-aging and anti-wrinkle effects by removing harmful active oxygen and protecting skin cells, and keeping the skin elastic and bright by containing the extract and the extract as an active ingredient in a cosmetic base .

Aging is an indispensable part of life. All creatures, whether animals or plants, are born on the path of aging. Thanks to hygiene and health care, human longevity has been significantly extended over the last century. As a result, the proportion of elderly people is steadily increasing. The aging process takes place in the entire body of the body, and the physiological functions of the organs, such as hypertension, chronic coronary artery disease and diabetes, are changed with aging (Choi, Hyun-Cheol, Of the dermatologists in Korea. Skin aging is also progressing with age.

Skin aging can be divided into natural aging (intrinsic aging) and surrounding environment, especially photoaging due to ultraviolet rays, which occurs naturally with age. Photoaing refers to the aging phenomenon observed in the skin such as the face, the back of the hand, and the back of the neck exposed to sunlight for a long time, which is a result of combining the effects of the endogenous aging and the ultraviolet rays. There is a difference in the mechanism of wrinkle formation between natural aging and photoaging, but the skin cell proliferation and metabolism are reduced, the defect between epidermis and dermis is weak, and it is easily damaged by small stimulus, Physiological changes such as excessive expression of collagenase, Matrix Metalloproteinase (MMPs), appear on the skin, and dryness of the skin is lost due to structural differences in the epidermis and dermis, resulting in wrinkles gradually becoming wrinkles.

Skin wrinkles are the most common symptom of aging skin, and are the most common criteria for assessing the degree of aging (Lee, Hyun-hwa (1999). A review of wrinkles and their management in skin aging. -631). As the living standards have improved recently and the interest in skin beauty has increased, interest in skin aging has increased. Therefore, although it is impossible to prevent skin aging, various attempts have been made to suppress and slow down skin wrinkling, which is a representative symptom of skin aging.

A potent mechanism for aging is known as oxidation of the body or 'oxidative stress' due to the formation of free radicals. Free radicals are made from almost all molecules when they lose electrons. It is produced in the mitochondria during cellular respiration when free energy in the cell binds stable oxygen. Cells have enzymes that convert oxygen to reactive oxygen species (ROS), superoxide (O ₂ -) or hydrogen peroxide (H ₂ O ₂ ). However, the activity of these enzymes is significantly lower than that of the mitochondrial respiratory chain. The human mitochondria take up 400 L of O ₂ per day and convert to water, which is a four-electron reduction process. If this part of the O ₂ single e-even if only O ^2- generated by reducing the amount of the path exceeds the ROS produced to take all other mechanisms. These ROS rapidly associate with other cell compounds and damage such as mitochondria and proteins. Antioxidants act to neutralize free radicals and to neutralize damage from free radicals.

A study of free radical theory on aging was made more active by superoxide dismutase (SOD) in 1956 (Denham H (1956) Aging: a theory based on free radical and radiation chemistry. Journal of Gerontology 11: 298-300 ).

Superoxide dismutase (SOD) causes the superoxide anion to change to oxygen (O ₂ ) and H ₂ O ₂ (hydrogen peroxide) by throwing or accepting electrons of Mn or Cu. . It is believed that the life span can be extended depending on the SOD enzyme activity ratio and gene expression. Over time, oxidative damage persists in cells and tissues, causing many changes in the body that occur as a result of aging. Free radicals are not only associated with aging but also with degenerative diseases such as cancer, atherosclerosis, and cataracts. Free radicals may be caused by external causes of skin aging, such as smoking or UV damage. In such cases, the skin's own antioxidant network, such as those found in Vitamin E11 and C, protects against damage by helping to eliminate free radicals. However, free radicals do not work well because of the lack of antioxidants to overcome the free radical generation, which damages the skin. Skin has a repair mechanism for this damage. However, as the time elapses, the repair mechanism gradually weakens, leading to cell death and apoptosis, resulting in skin aging.

In addition, ROS (Reactivoxygen species) causes adverse effects on cells. Since ROS oxidizes unsaturated fats in the fluid film surrounding the cells (called lipid peroxidation), the fluid membrane does not adequately protect the cells. Because nutrients can not pass through cells, waste accumulates and cells can not function efficiently. Some kinds of oxidized fats are converted into Aldehyde (toxic chemicals) that enter the cell, causing them to damage, causing an inflammatory reaction, and eventually causing apoptosis that will kill or suicide young, healthy skin cells. In addition, ROS increases collagenase, a protein enzyme that breaks down the supporting tissues of the skin, reducing the firmness of the skin.

In order to prevent aging of the skin, various attempts have been made such as laser treatment and facial molding. Conventional patents related to anti-aging cosmetic compositions using natural extracts, which are not an artificial method for aging as described above, are disclosed in Korean Patent Publication No. 10-0562348 B1 " High-functional cosmetic composition containing whitening, wrinkle and anti-aging cosmetic ingredients containing ethanol extract of Maize Huang, " Korean Registered Patent No. 10-0588830 B1, " Anti-aging cosmetic composition containing Baekjong extract, 10-0443588 B1 " Anti-aging cosmetic composition containing mulberry extract and ragweed extract ", Korean Patent Registration No. 10-0536744 B1 " Cosmetic composition having anti-aging effect on skin containing hornbug extract, There are many. However, there is no cosmetic composition containing the extract and the extract as main components.

Korean Patent Publication No. 10-0562348 B1 Korean Patent Registration No. 10-0588830 B1 Korean Patent Registration No. 10-0443588 B1 Korean Patent Publication No. 10-0536744 B1

The present inventors have made extensive efforts to develop a cosmetic composition having excellent antioxidation, anti-aging and anti-wrinkle effect by using various natural extracts. As a result, it has been found that cosmetic compositions containing Hylocereus undatus (Haw.) Britt. & Rose ) When applied to a composition, it has an antioxidative effect by removing active oxygen in the skin and protecting the skin from cell damaging substances, promoting the proliferation of skin cells and enhancing collagen biosynthesis, The present invention has been completed.

In order to verify the above-mentioned effects, the inventors of the present invention have found that extracts containing at least one of apple mango extract, fashion fruit extract, dragon extract, red kiwifruit extract and Atetomoya extract are extracted with a solvent and fractionated, and then Superoxide dismutase SOD) -like activity measurement method, the effect of promoting total collagen biosynthesis in the cell and the effect of promoting procollagen type-1 production from human fibroblast, Antioxidation, anti-aging and wrinkle-improving effects, thus completing the present invention.

Accordingly, an object of the present invention is to provide an extract showing antioxidative, anti-aging and anti-wrinkle effects.

Another object of the present invention is to provide a cosmetic composition comprising the above-mentioned extract.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

The present invention contains an extract of Hylocereus undatus (Haw.) Britt. & Rose ) as an active ingredient.

The extract may be an extract obtained by extracting with a solvent selected from water, alcohol, methanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, chloroform, glycerin and a mixed solvent thereof.

The extract may be extracted using at least one extraction method selected from the group consisting of heating at 45 to 120 ° C for 30 minutes to 24 hours followed by hot water extraction or shaking at 10 to 80 ° C for 6 to 24 hours.

The extract can be lyophilized at -60 to -10 < 0 > C for 24 to 72 hours.

The extract may be contained in an amount of 0.001 to 30.0% by weight, more preferably 0.01 to 5.0% by weight, based on the total weight of the cosmetic composition.

The extract may be for antioxidant, anti-aging and wrinkle improvement.

The cosmetic composition may have formulations of softening longevity, nutritional lotion, eye cream, nutritional essence, nutritional cream, massage cream, body cream, cleansing cream, cleansing foam, cleansing water, powder and pack.

The cosmetic composition further comprises one or more extracts selected from the group consisting of Mangifera indica L. , Passiflora edulis Sims , Red kiwi ( Actinidia chinensis Planch. ) And Annona Squamosa .

The dragon and extract of the present invention has an antioxidative effect by removing active oxygen in the skin and protecting the skin from cell damaging substances and promoting collagen biosynthesis by promoting the proliferation of skin cells, It is possible to prepare a cosmetic composition which exhibits excellent antioxidation, anti-aging and wrinkle-reducing effects by containing the above-mentioned extract and the extract as an active ingredient in a cosmetic base.

FIG. 1 is a graph showing the results of a superoxide dismutase (SOD) -like activity experiment of apple mango extract prepared in Example 1. FIG.
FIG. 2 is a graph showing the results of a superoxide dismutase (SOD) -like activity experiment of the fashion fruit extract prepared in Example 2. FIG.
FIG. 3 is a graph showing the results of a superoxide dismutase (SOD) -like activity experiment of the extract prepared in Example 3. FIG.
FIG. 4 is a graph showing the results of a superoxide dismutase (SOD) -like activity experiment of apple mango extract prepared in Example 4. FIG.
FIG. 5 is a graph showing the results of Superoxide dismutase (SOD) -like activity experiment of the fashion fruit extract prepared in Example 5. FIG.
6 is a graph showing the results of Superoxide dismutase (SOD) -like activity experiment of the fashion fruit extract prepared in Example 6. FIG.
FIG. 7 is a graph showing the results of the hydrogen peroxide removal effect of the apple mango extract prepared in Example 1. FIG.
8 is a graph showing the results of hydroperoxide removal effect of the fashion fruit extract prepared in Example 2. FIG.
FIG. 9 is a graph showing the results of hydroperoxide removal effect of the extract prepared in Example 3. FIG.
FIG. 10 is a graph showing the results of hydroperoxide removal effect of apple mango extract prepared in Example 4. FIG.
11 is a graph showing the results of the hydrogen peroxide removal effect of the fashion fruit extract prepared in Example 5. FIG.
FIG. 12 is a graph showing the results of hydroperoxide removal effect of the fashion fruit extract prepared in Example 6. FIG.

The present invention provides a cosmetic composition comprising an extract and an extract as an active ingredient.

The cosmetic composition according to the present invention may contain one or more extracts selected from the group consisting of Mangifera indica L. , Passiflora edulis Sims , Red kiwi ( Actinidia chinensis Planch. ) And Annona Squamosa May be further included.

In the present invention, the term 'extract' refers to the extracts of Mangifera indica L. , Passiflora edulis Sims , Hylocereus undatus (Haw.) Britt. & Rose ), Red kiwi ( Actinidia chinensis Planch. Refers to an extract obtained by extracting at least one species selected from the group consisting of hot water extraction using a solvent or shaking extraction with at least one selected from Annona Squamosa and then filtering and lyophilizing the extract .

Dragonfruit (Scientific name: Hylocereus undatus (Haw.) Britt. & Rose ) is a name given to the appearance that the fruit is open on the branches and resembles the figure holding the dragon. It is also known as Pitaya in English. One of the cactus fruit, origin is Central America. Vietnam, Taiwan, China, Thailand, and Japan, and is cultivated as a special product in Jeju Island in Korea.

It grows up to 50㎝ in size from the potted seedlings growing in the pot, and after about 10 months, the stem grows more than 1m and blooms. The flower is about 45㎝ long, full bloom overnight and then loses the next day. The fruit is elliptical, and it can be harvested 30 to 40 days after the petal has fallen. Magnesium, calcium, iron, zinc, sodium, carotene, water soluble dietary fiber, carbohydrate, protein, lipid, vitamin C, vitamin B1, and vitamin B2, which are superior to apples and pears, , Vitamin B3 and other beneficial minerals and antioxidants are rich in the body. Sugar content is 16-18 Brix (unit of sugar), higher than apple (8-16 Brix), peach (7-15 Brix) and citrus (7-16 Brix).

The varieties are white, yellow, and yellow. Cauliflower is red in the skin but the flesh is in the white variety, and red ginseng is the red variety in the skin and pulp. The yellow bell is yellow with flesh and the flesh is white. In Cheju Island, only white and white breeds are cultivated. White meat can be cut into several pieces and peeled like bananas, and red meat breeding is best to eat with a spoon because the pigment of fruit is buried in the hands. It is good to mix with water, milk, yogurt, honey, and change into a blender. It is also used as a main ingredient in making lacquer and jelly.

Apple Mango (Scientific name: Mangifera indica L. ) is a mango with red color like apples. Mangoes are classified as belonging to the lacquer family, and are known as the Malay Peninsula, Myanmar, and northern India. The cultivation history of mangoes is very long, 4000 years or 6000 years, and it is said to be a sacred fruit tree in India, which is a country of origin. Legend has it that Buddha is meditation, where enlightenment is said to be a bamboo, and this bamboo is a mango tree. It is also the world's most cultivated tropical fruit and its production is fifth in the world after grapes, bananas, oranges and apples. It was introduced in Europe after the Portuguese discovered the Indian route in the 15th century. By the Portuguese to Africa, to the conquered Brazil, along the islands to the West Indies, and to Mexico. Florida. California. Hawaii and so on. Currently, the distribution area is cultivated in tropical and subtropical regions such as Africa, Brazil, Mexico, Florida, California, Hawaii and is cultivated in Seogwipo, Jeju Island in Korea.

Jeju-grown mangoes are varieties of apple mangoes, which are produced in subtropical areas, and are highly adaptable to domestic soil. Also, the temperature difference between day and night is excellent. Especially, foreign fiber has a lot of fiber, thick skin and seed, whereas the improved Korean mango has a small seed and a thin shell. It also makes high sugar content and unique and 18bx.

Passiflora ( Passiflora edulis Sims ) is a tropical fruit with a clock flower. Fashion Fruits is a compound word of 'passion' and 'fruit'. It has a distinctive flavor and flavor. Its origin is southern Brazil. Each node of the stem has tendrils and stipules. Leaves are alternate phyllotaxis and three folded palmate compound leaves. The flower runs on the axilla of the branch of the new branch one by one, and blooms in the morning and it leaves in the afternoon. There are 5 calyxes, 5 white, 5 petals and purple spots on the white background. There are five stamens and three broken pistils. The fruit shape is round or elliptical, and there are those that ripen in red and yellow and ripen in yellow. When we divide the halves, we have lots of seeds, and the jelly state surrounding it is pulp. Flesh is sweet, but sour taste is strong enough to be processed with juice or jam. It uses fruit juice or syrup to make jelly, mousse, sorbet, and ice cream.

Fashion fruit is smaller in size but contains more ingredients for women than pomegranate. Niacin skin moisturizing effect 5.2 times that of pomegranate, dietary fiber to help intestinal bowel activity 2.6 times the pomegranate, helps relieve fatigue and vitamin C to improve the skin contains three times. Beta carotene (vitamin A), which has antioxidant properties, is not found in pomegranate, while fashion fruit is 64 ㎍ (per 100 g). On the other hand, fat is 58% less than pomegranate, and sugar is only 84%, so there is no worry about calories. It is therefore worth mentioning that the name "fruit of women" goes beyond the pomegranate and is called the "goddess fruit."

Jeju's fashion flesh is cultivated by environment-friendly farming methods that farmers bring in from overseas, eat soil, wind and sunlight of Jeju Island, and it is evaluated as a nutritious, fresh and fragrant fruit with excellent moisturizing, antioxidant and fatigue effect.

Red Kiwi (Scientific name: Actinidia chinensis Planch. ) Is a deciduous vine plant of the Quercus variabilis. Kiwi was originally a plant that grew in southern China, but it began growing in New Zealand in the early 20th century. In the early days, it was commercialized under the name 'Chinese gooseberry'. 'Hayward' varieties or similar varieties are generally cultivated, but gold-colored kiwi, with a yellow flesh, is a hybrids of dahlia and kiwi. The name Kiwi was attached to Kiwi, a New Zealand native bird. The official name is Chinese dyer, which is commonly known as kiwi. In China, it is also called Guido (鬼 桃), 里 梨 (藤 梨).

Flowers bloom in June-July and are white. Fruits are mature in 8-10 months, ovate, dense brown hairs and stored for 3-4 months. The center of the pulp is cream-colored and has semicircular seeds around it, light green around it and small black seeds embedded in it. Vitamin C is abundant, and one adult is required to have enough amount of one day for an adult. Kiwi contains six times as much vitamin E as apple, so it is effective in preventing heart disease and cancer. In addition, it contains polyphenols and polysaccharides, which are known to be strong antioxidants that inhibit the development of cancer.

One kiwi contains about 15% of the recommended daily dose of potassium, an electrolyte component that is involved in the function of the nerves and muscles, preventing hypertension and controlling heart activity and function. The taste seems to be a mixture of strawberry, banana and pineapple, but it is sour if it does not undergo enough aging period after harvest. Because of this, kiwi are classified as fresh fruits.

Red kiwi cultivated in Jeju is like gold or green kiwi, but it has little outer surface and fluffy hair. Red Kiwi is a delicious kiwi, and as soon as it is picked up, the sugar content is 15 ~ 16Brix. If it is cooked at room temperature for about one week, it goes over 20Brix.

Ahtte Moya (atemoya scientific name: Annona Squamosa) is the world's three migwa one (durian, mangosteen, cherimoya) is very rare in the world as one of the cherimoya fruit created by crossing a Sugar Apple Fruit. Atelope is a fruit called cherimoya in the Spanish language custard apple in the English language. Atemoya is a fruit of high sugar content without any acidity.

Atemoya is a blend of sweetsop, a tropical fruit, and Cherimoya, a highland of the Andes mountains. It is a combination of Brazilian 'Atele' in Sweet Shop and 'Moya' in Cherimoya and 'Atemoya' It is a fruit named as. The sugar content is about 25 Brix and boasts the highest sugar content of the fruit on the earth. Atemoya is a fruit that grows like Apple Mango, and it is best to eat it when you are tender and tender. Postpartum is naturally grown at room temperature for 5 ~ 6 days, and the color becomes dark brown. In order to promote postnatal growth, it is faster than natural postpartum if it is kept at room temperature in an enclosed space with apples.

Atemoyas are used in folk remedies for various plant parts. It is mainly caused by acetogenins, alkaloids and flavonoids contained in roots, stems, bark, fruit and seeds. Acetogenins are effective for anticancer efficacy and cytotoxicity. Flavonoids are also used as a preventive agent to inhibit tumor development. Seeds are used as folk remedies such as pesticides and insect repellents through ethanol extermination. Seeds are said to give a treatment effect of atropine because of strong toxicity.

In particular, 16-B and 17-dhyroxykauran-19-oic acid have been found to inhibit HIV, and alkaloids extracted from the leaves are known to have stabilizing, sleeping effects and Parkinson's symptoms in West India.

The cosmetic composition according to the present invention contains an extract ( Hylocereus undatus (Haw.) Britt. & Rose ) as an active ingredient.

According to a preferred embodiment of the present invention, the extract is extracted with an extraction solvent selected from water, alcohol, methanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, chloroform, glycerin, , And heating at 45 to 120 ° C for 30 minutes to 24 hours for hot water extraction or shaking at 10 to 80 ° C for 6 to 24 hours is used to improve the yield of the extract Which is preferable in terms of reliability. At this time, it is preferable that the extract is filtered step by step using a filter cloth, a filter paper and a vacuum filtration apparatus, and freeze-dried at -60 to -10 ° C for 24 to 72 hours.

According to a preferred embodiment of the present invention, the extract extracted from the above method may contain 0.001 to 30.0% by weight, more preferably 0.01 to 5.0% by weight, based on the total weight of the cosmetic composition. If the amount of the extract is less than 0.001% by weight based on the total weight of the whitening cosmetic composition, the function as a skin cosmetic material is deteriorated because the effect of antioxidation, anti-aging and wrinkle improvement can not be sufficiently obtained. There is a problem of safety due to such a problem.

The extract prepared from the above method exhibits remarkable antioxidation, anti-aging and anti-wrinkle effects and can provide a cosmetic composition having excellent effects.

The whitening cosmetic composition may be a formulation of a softening agent, a nutritional lotion, an eye cream, a nutritional essence, a nutritional cream, a massage cream, a body cream, a cleansing cream, a cleansing foam, a cleansing water, a powder and a pack.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

[Example]

Example 1. Preparation of hot water extract

500 g of distilled water (sample: solvent = 1: 10 wt / wt) was added to each 50 g of crushed apple mango, fashion fruit, dragon fruit, red kiwi and Atemoya and boiled for 10 hours at 100 ° C using a hot water extractor equipped with a cooling condenser After cooling to room temperature, the medicinal material was filtered with a 380 mesh filter cloth and filtered through a 11 μm paper filter. The filtrate was filtered through a 0.45 μm membrane filter under reduced pressure, and then lyophilized at -50 ° C. for 72 hours to obtain a final extract.

Example 2. Preparation of methanol extract

1000 g of 99.9% methanol (sample: solvent = 1: 20 wt / wt) was added to 50 g each of crushed apple mango, fashion fruit, dragon fruit, red kiwi and Attermoya and the mixture was subjected to third extraction at room temperature for 3 days. The herbs were filtered with a 380 mesh filter bag and the filtrate was collected. The remaining herbs were extracted with the same amount of 70% ethanol and extracted at room temperature for 3 days. The herbs were filtered with a 380 mesh filter, and the filtrate was collected. Finally, the filtered herbs were extracted with 70% ethanol equivalent, extracted at room temperature for 3 days, filtered with 380 mesh filter, and filtered with a paper filter with 11 μm filter paper. The final filtrate was filtered through a 0.45 μm membrane filter, followed by concentration under reduced pressure, followed by lyophilization at -50 ° C. for 72 hours to obtain a final extract.

Example 3: Preparation of ethanol extract

1,000 g of 70% ethanol (sample: solvent = 1: 20 wt / wt) was added to 50 g each of crushed apple mango, fashion fruit, dragon fruit, red kiwi and Atemoya. The herbs were filtered with a 380 mesh filter bag and the filtrate was collected. The remaining herbs were extracted with the same amount of 70% ethanol and extracted at room temperature for 3 days. The herbs were filtered with a 380 mesh filter, and the filtrate was collected. Finally, the filtered herbs were extracted with 70% ethanol equivalent, extracted at room temperature for 3 days, filtered with 380 mesh filter, and filtered with a paper filter with 11 μm filter paper. The final filtrate was filtered through a 0.45 μm membrane filter, filtered and concentrated under reduced pressure, and then lyophilized for 72 hours at -50 ° C to obtain the final extract.

Example 4. Preparation of ethyl acetate extract

100g of 99.9% ethyl acetate (sample: solvent = 1: 20 wt / wt) was added to 50g each of crushed apple mango, fashion fruit, dragon fruit, red kiwi and atemoya. The herbs were filtered with a 380 mesh filter bag and the filtrate was collected. The remaining herbs were extracted with the same amount of 70% ethanol and extracted at room temperature for 3 days. The herbs were filtered with a 380 mesh filter, and the filtrate was collected. Finally, the filtered herbs were extracted with 70% ethanol equivalent, extracted at room temperature for 3 days, filtered with 380 mesh filter, and filtered with a paper filter with 11 μm filter paper. The final filtrate was filtered through a 0.45 μm membrane filter, filtered and concentrated under reduced pressure, and then lyophilized for 72 hours at -50 ° C to obtain the final extract.

Example 5: Preparation of Butylene Glycol Extract

30g of butylene glycol 1000g (sample: solvent = 1: 20 wt / wt) was added to 50g each of crushed apple mango, fashion fruit, dragon fruit, red kiwifruit and Attermoya and extracted at room temperature for 5 days. After 5 days, the fruit was filtered with a 380 mesh filter cloth and filtered through a 11 [mu] m paper filter. The filtrate was filtered through a 0.45 μm membrane filter, and the final extract was obtained.

Example 6. Preparation of Propylene Glycol Extract

50 g of Propylene glycol 1000 g (sample: solvent = 1: 20 wt / wt) is added to 50 g each of crushed apple mango, fashion fruit, dragon fruit, red kiwi and Attermoya and the mixture is extracted at room temperature for 5 days. After 5 days, the fruit was filtered with a 380 mesh filter cloth and filtered through a 11 [mu] m paper filter. The filtrate was filtered through a 0.45 μm membrane filter, and the final extract was obtained.

[Experimental Example]

Experimental Example 1. Antioxidant effect

1-1. DPPH (2,2-Diphenyl-1-picrylhydrazyl) scavenging activity experiment

 100 μl of 5 μM to 10 -4 M DPPH (1-1-diphenyl-2-picryl-hydrazyl) was added to 100 μl of each concentration solution in all the examples, stirred for 1 minute, reacted for 30 minutes in a 25 ° C. incubator, Absorbance was measured at 540 nm using a microplate counter. L-Ascorbic aicd (100 μg / ml) was used as the experimental control, and the electron donating ability was shown by the absorbance reduction rate of the sample solution and the no-added solution.

The results of the antioxidative effects are shown in Tables 1 to 6 below.

Example 1. DPPH scavenging activity (%) Samples (占 퐂 / ml) Control 1000 100 10 Apple mango 100 82 48 13 Fashion Fruits 100 79 57 7 Dragon 100 90 68 35 Red Kiwi 100 37 18 4 Atemoya 100 42 11 3

Example 2 DPPH scavenging activity (%) Samples (占 퐂 / ml) Control 1000 100 10 Apple mango 100 50 35 14 Fashion Fruits 100 91 45 10 Dragon 100 85 55 27 Red Kiwi 100 67 12 9 Atemoya 100 78 20 4

Example 3. DPPH scavenging ability (%) Samples (占 퐂 / ml) Control 1000 100 10 Apple mango 100 57 38 4 Fashion Fruits 100 89 57 27 Dragon 100 94 68 35 Red Kiwi 100 59 15 14 Atemoya 100 48 31 21

Example 4. DPPH scavenging ability (%) Samples (占 퐂 / ml) Control 1000 100 10 Apple mango 100 91 56 33 Fashion Fruits 100 78 37 22 Dragon 100 68 34 15 Red Kiwi 100 57 28 12 Atemoya 100 40 15 9

Example 5. DPPH scavenging ability (%) sample(%) Control 4 2 One Apple mango 100 52 21 11 Fashion Fruits 100 59 47 13 Dragon 100 35 24 5 Red Kiwi 100 47 29 16 Atemoya 100 49 21 5

Example 6. DPPH scavenging ability (%) sample(%) Control 4 2 One Apple mango 100 58 35 10 Fashion Fruits 100 62 50 9 Dragon 100 60 55 6 Red Kiwi 100 30 25 19 Atemoya 100 52 15 8

As a result, as shown in Tables 1 to 6, DPPH elimination ability was significantly exhibited when they were treated by concentration in all the examples. Particularly, in Examples 1 and 4, apple mango, in Examples 2, 5 and 6, fashion fruit and in Example 3, the highest antioxidative activity was shown due to the DPPH scavenging ability being the highest. Examples 1, 2, 3 and 4 In Examples 5 and 6, the DPPH scavenging ability was generally low.

1-2. Superoxide dismutase (SOD) -like activity experiment

SOD-like activity was measured for the apple mango extracts (Examples 1 and 4), the fashionable fruit extract (Examples 2, 5 and 6), and the extract and the extract (Example 3) Was carried out as follows. 200 μl of WST working solution in SOD assay kit (WST) was added to 20 μl of the concentration-dependent sample solution, mixed with 20 μl of enzyme working solution, and incubated at 37 ° C for 20 minutes. Absorbance was measured at 450 nm using a microplate counter.

Superoxide dismutase (100 units, Sigma) was used as an experimental control, and the superoxide dismutase - like activity was expressed by the absorbance reduction rate of the sample solution and the non - additive solution.

Results for SOD (superoxide dismutase) -like activity measurements are shown in Table 7 and Figures 1-6 below.

SOD-like inhibition activity (%) Example 1.
Apple mango extract Example 2.
Fashion Fruits
extract Examples 3,
Dragon
extract Example 4.
Apple mango extract Example 5.
Fashion Fruit Extract Example 6.
Fashion Fruit Extract SOD (100 units) 98.25 98.25 94.25 93.75 97.75 97.75 10 (ug / ml) 45.2 17.95 7.95 12.8 7.75 12.75 25 (ug / ml) 72.15 29.95 39.95 30.25 12.95 24.90 50 (ug / ml) 90.25 46.8 57.30 64.85 25.80 34.30 100 (ug / ml) 92.80 56.65 74.15 88.50 36.50 46.50

As a result, as shown in Table 7 and Figs. 1 to 6, the apple mango extracts of Examples 1 and 4 had the best SOD-like activity. Next, the extracts of Example 3 and Activity.

1-3. Hydrogen peroxide removal effect

(Example 1, 4), the fashion fruit extract (Examples 2, 5 and 6), and the extract and the extract (Example 3), which exhibited the best effects from the respective results of the Experimental Example, the hydrogen peroxide removal effect The experiment was carried out as follows. In the experiment to remove the hydrogen peroxide, 20 μl of sample solution per concentration, 20 μl of 10 mM hydrogen peroxide, and 100 μl of 0.1 M phosphate buffer (pH 5) were added to a 96-well plate and reacted at 37 ° C. for 5 minutes. / Ml peroxidase was added and reacted at 37 ° C for 10 minutes, and the absorbance was measured at 405 nm.

The results of the hydrogen peroxide removal effect are shown in Table 8 and Figs. 7 to 12.

H ₂ O ₂ inhibition effect (%) Example 1.
Apple mango extract Example 2.
Fashion Fruits
extract Examples 3,
Dragon
extract Example 4.
Apple mango extract Example 5.
Fashion Fruit Extract Example 6.
Fashion Fruit Extract ascorbic
acid
(500 ug / ml) 99.67 100.17 100.00 97.00 98.00 97.67 25 (ug / ml) 11.23 8.83 9.90 5.27 5.27 6.13 50 (ug / ml) 23.97 17.70 21.00 9.99 8.50 9.83 100 (ug / ml) 38.60 35.17 34.17 22.33 20.40 15.50 250 (ug / ml) 75.00 48.00 85.00 39.30 36.67 24.00 500 (ug / ml) 86.83 60.33 93.00 50.50 45.47 30.50

As shown in Table 8 and FIGS. 7 to 12, the hydrogen peroxide removal efficiency test showed that when treated with the apple mango extract of Example 1 and the solvent extract of Example 3, The antioxidant activity was determined to be excellent.

Experimental Example 2: Cytotoxicity test

Based on the results obtained from the above-mentioned antioxidant experiments, the cytotoxicity for determining the effective concentration for measuring the promoting effect of total collagen and Procollagen type-I biosynthesis on human-derived dermal fibroblasts, which is an evaluation of wrinkle for each sample, was measured.

Human fibroblast cells were cultured in 10% FBS-IMDM (Iscove's Modified Dulbecco's Medium, Gibco), and the cell concentration was adjusted to 5 ㅧ 104 cells / ㎖. , And the cells were cultured in a 5% CO2 incubator at 37 ° C for 24 hours. After 24 hours of incubation, the medium was removed, and 200 μl of the extracts of Examples 1 to 6 prepared by 10% FBS-IMDM and 180 μl of FBS-free IMDM were added again and reacted for 48 hours under the same conditions. At this time, the experimental group using only FBS-free IMDM was used as a control of the sample. Three hours before the completion of the reaction, 20 쨉 l of a 5 mg / ml MTT solution (in 1 x PBS (-)) was added, and the reaction was further completed for 48 hours. After removing the MTT reaction solution, 150 μl of DMSO (Dimethyl sulfoxide) was added thereto, followed by stirring (room temperature) for 5 minutes. The absorbance was measured at 570 nm using a microplate counter (Bio-Rad, USA).

The results for the cytotoxicity experiment are shown in Tables 9 to 14 below.

Example 1. Cell proliferation rate (%) Samples (占 퐂 / ml) Control 200 150 100 50 Apple mango 100 103.12 108.89 112.54 83.92 Fashion Fruits 100 101.23 96.41 99.87 101.50 Dragon 100 84.35 97.04 83.49 100.42 Red Kiwi 100 142.30 112.42 110.83 97.15 Atemoya 100 88.01 91.08 72.49 100.85

Example 2. Cell proliferation rate (%) Samples (占 퐂 / ml) Control 200 150 100 50 Apple mango 100 101.02 99.84 115.01 89.24 Fashion Fruits 100 104.13 86.17 94.78 111.40 Dragon 100 88.50 95.45 100.56 92.10 Red Kiwi 100 132.12 110.21 97.48 99.75 Atemoya 100 84.27 91.58 82.59 120.90

Example 3. Cell proliferation rate (%) Samples (占 퐂 / ml) Control 200 150 100 50 Apple mango 100 162.30 112.42 99.83 97.15 Fashion Fruits 100 112.03 86.56 94.99 121.45 Dragon 100 105.15 98.05 93.55 90.72 Red Kiwi 100 160.10 92.47 115.97 99.25 Atemoya 100 94.20 91.18 92.45 90.85

Example 4. Cell proliferation rate (%) Samples (占 퐂 / ml) Control 200 150 100 50 Apple mango 100 95.10 88.01 98.55 90.52 Fashion Fruits 100 161.30 110.42 80.43 99.15 Dragon 100 94.35 100.04 93.19 89.12 Red Kiwi 100 92.30 102.42 90.83 97.15 Atemoya 100 102.57 51.08 72.59 88.45

Example 5. Cell proliferation rate (%) sample(%) Control 4 2 One 0.5 Apple mango 100 95.38 101.44 95.20 89.10 Fashion Fruits 100 91.23 86.51 109.17 111.45 Dragon 100 94.46 99.12 103.40 90.12 Red Kiwi 100 150.40 87.12 90.13 117.10 Atemoya 100 94.27 51.08 72.49 100.85

Example 6. Cell proliferation rate (%) sample(%) Control 4 2 One 0.5 Apple mango 100 93.52 98.87 102.56 99.10 Fashion Fruits 100 111.55 100.41 89.87 105.50 Dragon 100 89.55 99.04 93.56 100.45 Red Kiwi 100 102.40 92.00 100.83 97.45 Atemoya 100 80.16 80.45 85.49 86.85

As shown in Tables 9 to 14, the skin fibroblast cytotoxicity test results for Examples 1 to 6 showed that 200 μg / ml, 4% or less of all extracts from apple extracts of apple mango, fashion fruit, dragon, red kiwi and Atemoya But did not show cytotoxicity at the concentration. Particularly, as a result of the toxicity test of dermal fibroblast cells to Example 3, it was found that the effect of promoting cell proliferation on dermal fibroblasts was significant in a concentration-dependent manner.

Therefore, based on the test results, the effect of promoting collagen production from skin dermal fibroblasts was measured at a maximum concentration of 200 μg / ml and 4% or less.

EXPERIMENTAL EXAMPLE 3 Experiments to Promote Total Collagen Biosynthesis in Cells

Based on the results of Experimental Example 2, intracellular collagen production promoting effect was measured within an effective concentration that does not exhibit cytotoxicity.

In order to measure the promoting effect of collagen production in CCD-986sk (human skin dermal fibroblast), SIRCOL collagen assay (Biocolor Ltd) was used as follows. That is, after culturing the CCD-986sk (human dermal skin fibroblast) in 10% FBS-IMDM, the cell concentration was adjusted to 1.5 x 105 cells / ml and the cell lysate was prepared. And cultured for 24 hours at 37 ° C in a 5% CO2 incubator.

After culturing for 24 hours, the medium was removed, and 1 ml of fresh 10% FBS-IMDM was added. Each of the samples prepared by concentration was added again, and incubated again for 24 hours under the same conditions. After culturing for 24 hours, 0.5 ml of culture was taken from each plate, transferred to a microtube, and 1 ml of DYE Reagent (SIRCOL collagen assay, Biocolor Ltd) was added. After stirring at room temperature for 30 minutes, the supernatant DYE Reagent was completely removed by centrifugation (15,000 rpm, 5 minutes). Then, 0.1 ml of ALKALI Reagent (SIRCOL collagen assay, Biocolor Ltd) was added, stirred for 5 minutes, transferred to a 96-well microplate, and absorbance was measured at 540 nm using a microplate counter. El-ascorbic acid (100 μg / ml) was used as a comparator, and the promoting effect of collagen biosynthesis was shown by the absorbance increase rate relative to the no-added solution of the sample solution. Here, the control means no additive.

The results of experiments for promoting total collagen biosynthesis in the cells are shown in Tables 15 to 20 below.

Example 1: Total collagen biosynthesis promoting effect (%) Samples (占 퐂 / ml) Control Comparator 200 150 100 50 Apple mango 100 200 140.2 135.5 111.2 105.7 Fashion Fruits 100 200 103.7 100.8 98.4 97.5 Dragon 100 200 130.4 124.0 105.9 102.9 Red Kiwi 100 200 100.8 95.7 90.2 88.2 Atemoya 100 200 146.8 125.9 109.9 103.8

Example 2: Total collagen biosynthesis promoting effect (%) Samples (占 퐂 / ml) Control Comparator 200 150 100 50 Apple mango 100 200 150.2 146.5 110.5 103.1 Fashion Fruits 100 200 102.9 101.5 97.8 94.2 Dragon 100 200 100.6 99.8 90.8 84.7 Red Kiwi 100 200 152.9 134.7 124.6 116.2 Atemoya 100 200 136.4 122.4 119.2 101.4

Example 3: Total collagen biosynthesis promotion effect (%) Samples (占 퐂 / ml) Control Comparator 200 150 100 50 Apple mango 100 200 143.8 129.5 120.2 111.7 Fashion Fruits 100 200 105.1 102.1 98.8 89.5 Dragon 100 200 148.4 138.5 124.6 116.9 Red Kiwi 100 200 99.5 95.6 83.1 81.1 Atemoya 100 200 142.1 125.2 119.8 107.2

Example 4. Promoting effect of total collagen biosynthesis (%) Samples (占 퐂 / ml) Control Comparator 200 150 100 50 Apple mango 100 200 162.2 145.8 121.5 110.4 Fashion Fruits 100 200 104.9 102.8 95.1 89.9 Dragon 100 200 100.5 99.6 93.5 90.9 Red Kiwi 100 200 101.5 100.9 96.8 88.1 Atemoya 100 200 103.1 99.4 97.5 90.0

Example 5: Total collagen biosynthesis promoting effect (%) sample(%) Control Comparator 4 2 One 0.5 Apple mango 100 200 106.1 103.7 98.1 88.4 Fashion Fruits 100 200 103.9 97.5 93.6 85.5 Dragon 100 200 101.6 99.9 95.7 92.3 Red Kiwi 100 200 105.4 101.6 96.9 91.4 Atemoya 100 200 103.2 99.8 94.7 91.1

Example 6. Total collagen biosynthesis promoting effect (%) sample(%) Control Comparator 4 2 One 0.5 Apple mango 100 200 105.1 103.7 98.6 95.2 Fashion Fruits 100 200 102.8 98.5 90.8 88.2 Dragon 100 200 103.6 99.1 95.9 84.6 Red Kiwi 100 200 106.0 102.1 93.5 91.2 Atemoya 100 200 103.5 101.9 92.2 86.4

As shown in Tables 15 to 20, in the case of Example 1, in the case of Example 2, in the case of Example 2, in the case of Example 3, from the apple mango, red kiwi, and attemya extracts, the apple mango, In Example 4, Atemeya extract showed a concentration-dependent stimulation of collagen biosynthesis in Atemoya extract, which promotes the synthesis of collagen, which is a constituent in the skin, and is effective in improving skin aging and wrinkles. In Examples 4 and 5, total collagen biosynthesis was not exhibited. In the case of L-ascorbic acid (100 μg / ml) as a comparative example, the promoting effect was 200%.

Experimental Example 5: Promoting the production of Procollagen type-1 derived from human fibroblast

Based on the results of Experimental Examples 3 and 4, the effect of accelerating the production of intracellular Procollagne type-1 in the case of the extract of each Example without cytotoxicity and promoting total collagen production was measured.

In this experiment, the amount of propeptides that are separated when collagen is formed in Procollagen during the process of collagen production by human skin dermal fibroblast activity is tested to see if there is a wrinkle improving effect. The culture method of human skin dermal fibroblast CCD-986sk used in this experiment was the same as that of the isoflavone derivative of cytotoxicity measuring method. For the test method, 3 ㅧ 104 cells / ㎖ cell liquid was prepared with pre-cultured Fibroblast, Each well was plated in a 48-well plate and incubated in a CO 2 incubator (37 ° C, 5% CO 2) for 24 hours. After incubation, the medium was removed and washed once with 1 ml of PBS. 900 μl of FBS free IMDM medium was added, and 100 μl of the extract prepared by previously prepared concentration was also added and then cultured in a CO2 incubator (same condition) for 48 hours. When the culture was completed, 1.5 ml of each plate was taken and transferred to a microtube. 100 μl of the prepared culture was added to each well of a 96-well plate provided by Procollagen Type I C-peptide EIA kit (procoated), and then cultured in a CO2 incubator (same conditions) for 2 hours. After removing the culture medium from the well plate, wash with 400 μl of PBS three times, add 100 μl of Antibody-POD Conjugate Solution, react at 37 ° C for 1 hour, remove the well plate, wash with 400 μl of PBS three times Respectively. 100 μl of Substrate solution (TMBZ: tetramethylbenzidine) was added, reacted at room temperature (20-30 ° C) for 15 minutes, and 100 μl of stop solution (1N H ₂ SO ₄ ) was added and mixed well. Absorbance was measured at 540 nm using a microplate reader. The amount of collagen was determined by measuring the absorbance at 540 nm using the standard solution in the kit, and the amount of synthesized propeptide was calculated. The control means a non-additive.

The results for promoting the production of Procollagen type-1 from human fibroblasts are shown in Tables 21 to 24 below.

Example 1. Procollagen type-1 production promoting effect (ratio) Samples (占 퐂 / ml) Control Comparator 200 150 100 50 Apple mango 1.0 2.5 2.15 1.60 1.24 1.12 Dragon 1.0 2.5 2.25 1.67 1.37 1.09 Atemoya 1.0 2.5 1.98 1.48 1.19 0.97

Example 2. Procollagen type-1 production promoting effect (ratio) Samples (占 퐂 / ml) Control Comparator 200 150 100 50 Apple mango 1.0 2.5 2.31 1.97 1.82 1.51 Red Kiwi 1.0 2.5 2.20 1.84 1.53 1.30 Atemoya 1.0 2.5 2.05 1.95 1.23 0.99

Example 3 Promoting effect of Procollagen type-1 production (ratio) Samples (占 퐂 / ml) Control Comparator 200 150 100 50 Apple mango 1.0 2.5 2.46 2.05 1.76 1.40 Dragon 1.0 2.5 2.21 1.45 1.20 0.97 Atemoya 1.0 2.5 1.95 1.74 1.39 1.02

Example 4. Procollagen type-1 production promoting effect (ratio) Samples (占 퐂 / ml) Control Comparator 200 150 100 50 Apple mango 1.0 2.5 2.61 2.25 1.96 1.66

As shown in Tables 21 to 24, fruit extract promoting total collagen production promoted Procollagen type-1 production. In Example 1, procelollen type-I production promoting effect of up to 2.31 times, 2.46 times and 2.61 times in apple mango extract was promoted up to 2.25 times in the extract of Example 1 and 2,000 times in the extracts of Example 2, 3 and 4, It was found that the effect of the present invention on the improvement of the wrinkles was definitely effective. In the case of L-ascorbic acid (100 μg / ml), the effect was 2.5 times.

Accordingly, at least one of the apple mango extract, the fashion fruit extract, the dragon extract, the red kiwifruit extract, and the Ateto Moya extract is oxidized by a series of antioxidative effects and confirmation of promoting the biosynthesis of collagen and collagen precursors in human fibroblasts It is possible to prevent aging by promoting collagen production, and to exert an effect of improving skin wrinkles.

Hereinafter, each cosmetic formulation contains at least one of apple mango extract, fashion fruit extract, dragon extract, red kiwifruit extract and Atte Moya extract to remove active oxygen in skin and protect skin from cell damaging substances And exhibits an effect of enhancing collagen biosynthesis by promoting the activity of skin cells, thereby exhibiting an example of a cosmetic composition as a formulation having effects on antioxidation, anti-aging and wrinkle improvement.

The following prescription examples relate to the apple mango extract of Example 4 which has the best antioxidation activity, promotes skin cell activity and promotes collagen production, but is not necessarily limited thereto.

[Prescription Example]

Prescription Examples 1. Flexible lotion

Table 25 shows examples of prescription of softening water in cosmetics containing apple mango extract of Example 4.

ingredient Content (% by weight) Example 4. Apple mango extract 0.02 Polyoxyethylene hardened castor oil 0.5 Glycine 3.0 Dipotassium glycyrrhizate 0.1 1,3-butylene glycol 3.0 Sodium hyaruronate 0.1 ethanol 5.0 Antioxidant 0.1 Triethanolamine 0.1 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription Example 2. Nourishing Lotion

The prescription examples of the nutritional lotion among the cosmetics containing the apple mango extract of Example 4 are shown in Table 26 below.

ingredient Content (% by weight) Example 4. Apple mango extract 0.02 glycerin 7.0 Sorbitan stearate sucrose cocoate 2.0 Mineral oil 4.0 Trioctanoin 1.0 Stearic Acid 1.0 Glyceryl stearate 0.5 Sorbitan monostearate 1.0 Dimethicone 0.5 Antioxidant 0.3 Triethanolamine 0.1 Carbomer 0.2 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription Example 3. Essence

Table 27 shows prescription examples of essences in cosmetics containing apple mango extract of Example 4.

ingredient Content (% by weight) The apple mango extract of Example 4 0.02 glycerin 5.0 1,3-butylene glycol 2.0 Polyethylene glycol 2.0 Carbomer 1.0 Sodium hyaruronate 0.1 Glycine 3.0 Polyacrylamide 2.0 Hydroxyethyl cellulose 0.2 ethanol 3.0 Antioxidant 0.3 Triethanolamine 0.1 Polyoxyethylene hardened castor oil 1.0 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription Example 4. Cream

Table 28 shows examples of formulations of cream in cosmetics containing apple mango extract of Example 4.

ingredient Content (% by weight) The apple mango extract of Example 4 0.02 1,3-Buthylene Glycol 3.0 glycerin 3.0 Hydrogenated lecithin 1.0 Octyldodecanol 3.0 Trioctanoin 2.0 Stearic Acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan gum 0.2 Triethanolamine 0.1 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription Example 5. Pack

The prescription examples of the packs of the cosmetics containing the apple mango extract of Example 4 are shown in Table 29 below.

ingredient Content (% by weight) The apple mango extract of Example 4 0.02 glycerin 7.0 1,3-butylene glycol 3.0 Squalene 3.0 Dimethicone 3.0 Sodium hyaruronate 0.1 Glycine 2.0 Polyacrylamide 5.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Herein, each cosmetic formulation contains at least one of apple mango extract, fashion fruit extract, dragon extract, red kiwifruit extract and Atte Moya extract to remove active oxygen in skin and protect skin from cell damaging substances And exhibits an effect of enhancing collagen biosynthesis by promoting the activity of skin cells, thereby showing an example of cosmetic composition as a formulation having antioxidative and wrinkle-reducing effects, which is not limited to this formulation.

The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the formulas disclosed in the present invention are not intended to limit the scope of the present invention but to illustrate the present invention. The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

Claims (9)

A composition for improving wrinkles, comprising an extract of Hylocereus undatus (Haw.) Britt. & Rose as an active ingredient, the extract being extracted with water or ethanol.
delete The method according to claim 1,
Wherein the extract is extracted using at least one extraction method selected from the group consisting of heating at 45 to 120 ° C for 30 minutes to 24 hours followed by hot water extraction or shaking at 10 to 80 ° C for 6 to 24 hours Cosmetic composition.
The method according to claim 1,
Wherein the extract is lyophilized at -60 to -10 캜 for 24 to 72 hours.
The method according to claim 1,
Wherein the extract comprises 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.
The method according to claim 1,
Wherein the extract comprises 0.01 to 5.0% by weight based on the total weight of the cosmetic composition.
The method according to claim 1,
Wherein the extract further has an antioxidative effect.
The method according to claim 1,
Wherein the cosmetic composition has a formulation of a softening agent, a nutritional lotion, an eye cream, a nutritional essence, a nutritional cream, a massage cream, a body cream, a cleansing cream, a cleansing foam, a cleansing water, a powder and a pack.
The method according to claim 1,
Wherein the cosmetic composition further comprises one or more extracts selected from the group consisting of Mangifera indica L. , Passiflora edulis Sims , Red kiwi ( Actinidia chinensis Planch. ) And Annona Squamosa , Cosmetic composition.

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