KR102253376B1 - Composition for improving winkle or skin whitening comprising atriplex gmelinii extract or paederia scandens extract - Google Patents

Abstract

The present invention relates to a composition for wrinkle improvement or skin whitening comprising an extract of Atriplex gmelinii or Paederia scandens as an active ingredient. It provides a cosmetic composition and a health functional food composition for wrinkle improvement or skin whitening containing as an active ingredient.
The extract has antioxidant properties, and can exhibit excellent wrinkle improvement and whitening effects by inhibiting the activity of collagenase, elastase, and tyrosinase, and thus can be used as a functional cosmetic composition and a health functional food composition.

Description

A composition for improving wrinkles or skin whitening containing extracts such as chinensis or Gyeyo as an active ingredient {COMPOSITION FOR IMPROVING WINKLE OR SKIN WHITENING COMPRISING ATRIPLEX GMELINII EXTRACT OR PAEDERIA SCANDENS EXTRACT}

The present invention relates to a composition for improving wrinkles or skin whitening, comprising an extract of Atriplex gmelinii or an extract of Paederia scandens as an active ingredient.

Skin aging is a phenomenon that occurs through functional, structural, and biochemical processes in both body tissues and cells, and modern people seeking a healthy and relaxing life have increased interest in skin health, resulting in functional cosmetics derived from natural extracts for wrinkle improvement and whitening. There is a trend of increasing the preference of. In response to this, research and development on functional foods and functional cosmetic materials made of natural materials are actively progressing.

Reactive oxygen species (ROS), one of the causes of skin aging, are substances produced by various factors such as energy generation process in the body and exposure to ultraviolet rays, and fragmentation of DNA, peroxidation and inactivation of lipids and proteins, etc. It causes aging by reducing the biological function. Due to this ROS, human skin exhibits several functional and structural changes. Skin elasticity decreases due to extrinsic aging due to various contaminants and UV exposure, as well as intrinsic aging, which decreases the structure of the stratum corneum and biosynthesis of biological proteins. Freckles are produced.

The occurrence of skin wrinkles is a representative symptom of aging skin. The main cause is a remarkable decrease in collagen, a collagen protein produced from fibroblasts, and elastin, a resilient protein, of the skin dermis. Collagen is a major component of the extracellular matrix (ECM), which accounts for about 80% of the skin dermis. It forms most of the organic substances in the skin, tendons, bones and teeth. It serves to protect the body. Elastin, an insoluble fiber protein that contributes to elasticity, occupies 3-4% of the dermal layer and surrounds the collagen bundle. As skin aging progresses, the number and function of skin fibroblasts decrease, resulting in a decrease in the production of matrix proteins such as collagen and elastin, and the activities of collagenase and elastase that degrade them increase. Elasticity, moisture, and smoothness are slowed down.

Melanin (melanin) is widely distributed in living organisms, and is one of the major factors determining the color of human skin, hair, and eyes. Melanin is synthesized from melanocytes present in the epidermal layer of the skin and transferred to keratinocytes through dendrite, protecting the skin from harmful elements such as ultraviolet rays. Melanin production, a major factor causing abnormal skin pigmentation problems, is produced by enzymes such as tyrosinase, tyrosinase related protein-1 (TRP-1), and TRP-2. Therefore, by inhibiting the activity or synthesis of tyrosinase, melanin production can be reduced, and abnormal skin pigmentation problems such as melasma and freckles can be suppressed.

Currently, tyrosinase inhibitors such as hydroquinone, kojic acid, and arbutin are used as raw materials for whitening functional cosmetics, but problems related to skin safety such as cytotoxicity and skin cancer have been reported. There is a need to develop natural cosmetic materials.

Korean Patent Registration No. 10-0616252 (registered on August 21, 2006)

In order to solve the above problems, the present invention provides a cosmetic composition made of a safe natural material having excellent efficacy in improving wrinkles or skin whitening.

In addition, the present invention provides a health functional food composition made of a safe natural material having excellent efficacy in improving wrinkles or skin whitening.

The cosmetic composition for improving wrinkles or skin whitening according to the present invention may contain an extract of Atriplex gmelinii , an extract of Paederia scandens , or a mixture thereof as an active ingredient.

The health functional food composition for improving wrinkles or skin whitening according to the present invention may contain an extract of Atriplex gmelinii , an extract of Paederia scandens , or a mixture thereof as an active ingredient.

The extract of Atriplex gmelinii , Paederia scandens , or a mixture thereof according to the present invention has excellent antioxidant activity, is effective in improving skin wrinkles by inhibiting the activity of collagenase and elastase, and has tyrosinase activity. By inhibiting, it has properties that are effective in preventing or improving pigmentation.

Accordingly, cosmetic compositions and health functional food compositions containing the extract as an active ingredient exhibit excellent effects on wrinkle improvement or skin whitening, and thus can be usefully used as functional cosmetics or health functional foods.

In addition, the composition can be used safely from toxicity or side effects as a natural material.

1 is a graph showing the total content of polyphenols and flavonoids in a methanol extract (AGME) according to an experimental example of the present invention.
2 is a graph showing the total content of polyphenols and flavonoids in a methanol extract (PSME) such as Gyeyo according to an experimental example of the present invention.
Figure 3 is a graph showing the electron donating ability according to the concentration of the methanol extract (AGME) according to an experimental example of the present invention.
Figure 4 is a graph showing the electron donating ability according to the concentration of the methanol extract (PSME) such as gyeyo according to an experimental example of the present invention.
5 is a graph showing the inhibitory ability of collagenase activity according to the concentration of a methanol extract (AGME) according to an experimental example of the present invention.
6 is a graph showing the inhibitory ability of collagenase activity according to the concentration of a methanol extract (PSME) such as Gyeyo according to an experimental example of the present invention.
7 is a graph showing the inhibitory ability of elastase activity according to the concentration of the methanol extract (AGME) of the present invention according to an experimental example.
8 is a graph showing the inhibitory ability of elastase activity according to the concentration of a methanol extract (PSME) such as Gyeyo according to an experimental example of the present invention.
9 is a graph showing the ability to inhibit the tyrosinase activity according to the concentration of the methanol extract (AGME) of the tyrosinase according to an experimental example of the present invention.
10 is a graph showing the ability to inhibit the tyrosinase activity according to the concentration of methanol extract (PSME) such as gyeyo according to an experimental example of the present invention.

Hereinafter, the present invention will be described in detail.

The present inventors have selected Gaetneungja and Gyeyo as new natural material materials that require higher safety and functionality than existing natural plants, and functional cosmetics through various physiologically active features found in methanol extracts of Gyeyo, etc. The present invention was completed by confirming the possibility of application as a natural material.

In the present invention, " Atriplex gmelinii " is a salty plant belonging to the genus Chenopodiaceae, and refers to an annual herb that grows in intertidal zones, reclaimed lands, and coastal sand dunes, which are areas with high salinity along the coast. It is also distributed in Korea, Japan, China, Russia, and North America, and young leaves and stems have been used as food for livestock, and are also called "Gingamyeongaju".

In the present invention, the term " Paederia scandens " is a vine plant belonging to the Rubiaceae family, and grows wild in sunny places or beach grasses in the southern regions of Korea and Jeju Island, Vietnam, India, the Philippines, Japan, and China. , It is also distributed in the United States. In oriental medicine and in the private sector, it is known that the roots, leaves, skins and fruits of Gyeyo etc. remove wind, promote blood circulation, and detoxify.

In the present invention, "extract" refers to a substance obtained by extracting a component of a natural substance regardless of the extraction method, the extraction solvent, the extracted component, or the form of the extract. Any material that can be obtained by processing or processing by a method may be included.

In the present invention, "wrinkle improvement" refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating wrinkles that have already been created. Wrinkle generation is mainly caused by a decrease in collagen, and the decrease in collagen may be caused by excessive oxidative stress or ultraviolet irradiation.

In the present invention, "whitening" refers to an action of suppressing, inhibiting, or alleviating excessive deposition of melanin pigments and the like to prevent blackening of the skin caused by the pigmentation, thereby improving skin troubles.

In the present invention, the term "cosmetics" may include functional cosmetics having an effect of preventing or improving skin aging, and the like, and the functional cosmetics have a physiologically active effect and a professional functionality with an emphasis on the effect unlike general cosmetics. , As defined in the Cosmetics Act, it refers to a cosmetic that emphasizes specific effects and effects such as skin whitening and skin wrinkle improvement.

In the present invention, the term "health functional food" is a food manufactured and processed using raw materials or ingredients having useful functions for the human body, and is a food with high medical and medical effects processed so that a bioregulatory function is effectively displayed in addition to nutritional supply. It means and can be mixed with terms known in the art such as functional food.

The present invention provides a cosmetic composition for improving wrinkles or skin whitening, containing as an active ingredient an extract of Atriplex gmelinii , an extract of Paederia scandens, or a mixture thereof.

In the cosmetic composition according to the present invention, the extract or Gyeyo etc. extract may be extracted with water, C1 to C4 alcohol or a mixed solvent thereof, and preferably extracted using methanol or an aqueous methanol solution as a solvent. It may be, but is not limited thereto.

The extract or Gyeyo etc. extract may be extracted according to a method commonly used in the art, for example, hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc., and these are performed alone or two types It can be carried out using the above method in combination.

The cosmetic composition according to the present invention may contain as an active ingredient the single extract of the extract of Gaetneungji or Gyeyo, etc., and may contain a complex extract extracted by mixing the extract of Gyeyo, etc. as an active ingredient, or the It may contain as an active ingredient a mixture of the extract and the extract of Gyeyo and the like.

In the cosmetic composition according to the present invention, the extract or extract or mixture thereof may have antioxidant activity, and inhibit the activity of collagenase, elastase, or tyrosinase. can do.

According to an experimental example of the present invention, the methanol extract of slender mums was found to contain 268.7 TAE mg/g of polyphenol and 247.5 RE mg/g of flavonoids, and the methanol extract of Gyeyo etc. was found to contain 268.7 TAE mg/g of polyphenol and 247.5 RE mg/g of flavonoids. It contained 373.3 TAE mg/g polyphenol and 305.8 RE mg/g flavonoids, showing high antioxidant activity. The polyphenols and flavonoids are secondary metabolites of plants, and are antioxidants that can increase the safety of foods or cosmetics by inhibiting antioxidant activity and lipid peroxidation. In addition, the electron donating ability through DPPH radical scavenging activity of methanol extracts such as methanol extract, Gyeyo, etc. and ascorbic acid as a positive control were 83.2%, 91.5%, and 97.2% respectively at 1000 μg/mL, which was similar to the positive control. .

In the cosmetic composition according to the present invention, the extract or extract of Gyeyo, or a mixture thereof may exhibit an excellent wrinkle improvement effect by inhibiting collagenase or elastase activity. The anti-wrinkle effect may include conventional skin protective effects such as preventing or improving skin wrinkles, inhibiting or improving skin aging. In addition, collagen and elastin are involved in the mechanical firmness of the skin, the binding force and elasticity of the tissues, so the skin elasticity improvement, skin regeneration, wound healing, repair and regeneration of damaged skin tissues by the extracts of the slender snail extract, the extract or mixtures thereof Etc. can be obtained.

According to an experimental example of the present invention, the inhibitory activity of collagenase activity of the methanol extract, Gyeyo, etc., and ascorbic acid as a positive control thereof were 41.4%, 62.8%, and 69.7%, respectively, at 1000 μg/mL, and IC ₅₀ Was not calculated in the methanol extract, but was found to be greater than 1000 μg/mL, and the IC ₅₀ of methanol extract and ascorbic acid were calculated as 697.9 and 448.3 μg/mL, respectively.

In addition, the inhibitory activity of elastase activity of methanol extracts such as the methanol extract and the methanol extract of Gyeyo, and ascorbic acid as a positive control thereof were 36.9%, 56.0% and 40.5% respectively at 1000 μg/mL, and the IC ₅₀ of the methanol extract such as Gyeyo was 824.8 μg. It was calculated as /mL, and the IC ₅₀ of the methanol extract and ascorbic acid was not calculated, but it was found to be greater than 1000 μg/mL. Through this, it can be confirmed that the inhibitory ability of the methanol extracts such as the methanol extract and Gyeyo to inhibit the activity of collagenase or elastase is similar to or superior to that of the positive control group.

In the cosmetic composition according to the present invention, the tyrosinase is an enzyme involved in the process of producing melanin that induces pigmentation, and the extract, Gyeyo etc., or a mixture thereof inhibits the activity of the tyrosinase, thereby exhibiting excellent skin whitening effect. Can be indicated. The whitening effect may include freckles, melasma, hyperpigmentation after UV exposure, hyperpigmentation after inflammation, prevention of pigmentation including dark spots, brown spots, age spots, and the like.

According to an experimental example of the present invention, the inhibitory ability of tyrosinase activity of the methanol extract, the methanol extract such as Gyeyo, and arbutin, a positive control thereof, were 74.7%, 65.5%, and 73.1% at 1000 μg/mL, respectively, and the methanol extract of Gyeyo and the like _{The IC 50} of arbutin, a positive control group, was calculated as 126.3 and 175.3 μg/mL, respectively, and the IC ₅₀ of the methanol extract was not calculated, but it was found to be smaller than 62.5 μg/mL. It can be seen that the inhibitory ability of the methanol extract to inhibit tyrosinase activity is similar to or higher than that of the positive control arbutin. More details will be described later in the following experimental examples.

In the cosmetic composition according to the present invention, the extract, such as Gyeyo, or a mixture thereof may be included in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total cosmetic composition, but is not limited thereto.

The cosmetic composition according to the present invention may further include one or more of the ingredients registered in the list of cosmetic ingredients registered with the Food and Drug Administration and the ICID (International Cosmetic Material Collection). For example, the cosmetic composition may include organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, fragrances, surfactants, emulsifiers, fillers, sequestering agents, preservatives, vitamins, blockers, It may further comprise one or more adjuvants commonly used in the cosmetic field, such as wetting agents, dyes and pigments, hydrophilic or lipophilic actives, or any other ingredients commonly used in cosmetics.

The vitamins may include water-soluble or oil-soluble vitamins, and the water-soluble vitamins are water-soluble vitamins that can be blended into cosmetics, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, Nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like, and more preferably, vitamin C, which aids in collagen synthesis, may be included. In addition, their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-magnesium salt, etc.) may also be included, and the water-soluble vitamin is a microbial transformation method. , It can be obtained by a conventional method such as a purification method from a culture of microorganisms, an enzymatic method, or a chemical synthesis method.

The cosmetic composition according to the present invention can be prepared in any formulation conventionally prepared in the technical field to which the present invention belongs. For example, it may be formulated as a lotion, emulsion, lotion, cream, paste, gel, solution, suspension, oil, wax, pack, powder, foundation, spray, surfactant-containing cleansing, etc., but is not limited thereto. . More specifically, flexible lotion, nutrition lotion, astringent lotion, nutrition cream, massage cream, milk lotion, powder, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, powder foundation, emulsion foundation , Cleansing cream, cleansing foam, cleansing water, soap, pack, stick product, balm type product, spray or powder formulation.

When the cosmetic composition according to the present invention is in a cream or gel formulation, it further contains animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component. I can.

When the cosmetic composition is a solution or emulsion formulation, as a solvent, solvating agent or emulsifying agent, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or fatty acid of sorbitan It may further include esters and the like.

When the cosmetic composition is a suspension formulation, as a carrier component, liquid diluents such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline It may further include cellulose, aluminum metahydroxide, bentonite, agar, or tracant.

When the cosmetic composition is a powder or spray formulation, it may further include lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder as a carrier component, and in particular, in the case of a spray formulation, additional chlorofluorohydrocarbon , It may further include a propellant such as propane / butane or dimethyl ether.

When the cosmetic composition is a surfactant-containing cleansing formulation, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester.

The cosmetic composition according to the present invention may be used alone or in duplicate, or may be used in duplicate with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use may be varied according to a user's skin condition or taste.

In addition, the present invention provides a health functional food composition for improving wrinkles or skin whitening, containing as an active ingredient an extract of Atriplex gmelinii , an extract of Paederia scandens, or a mixture thereof.

The extract or a mixture thereof may have antioxidant activity, and may inhibit the activity of collagenase, elastase, or tyrosinase, thereby improving skin wrinkles or whitening skin. The wrinkle improvement effect may include conventional skin protection effects such as preventing and improving skin wrinkles, inhibiting and improving skin aging, and collagen and elastin are involved in the mechanical firmness of the skin, the binding force and elasticity of the tissue, and so on, improving skin elasticity, Effects such as skin regeneration, wound healing, and repair and regeneration of damaged skin tissue can be obtained. The skin whitening effect may include freckles, melasma, hyperpigmentation after UV exposure, hyperpigmentation after inflammation, prevention and improvement of pigmentation including senile black spots, brown spots or age spots.

In the health functional food composition according to the present invention, the health functional food may be prepared as a powder, granule, tablet, capsule, syrup or beverage, and there is no limitation on the form that the health functional food can take. It can include all foods of meaning. For example, beverages and various drinks, fruits and processed foods thereof (canned fruit, jam, etc.), fish, meat and processed foods thereof (ham, bacon, etc.), bread and noodles, cookies and snacks, dairy products (butter, cheese, etc.) ) And the like, and may include all functional foods in the usual sense. It may also include food used as feed for animals.

The health functional food composition according to the present invention may be prepared further comprising a food pharmaceutically acceptable food additive and other appropriate auxiliary ingredients commonly used in the art. For example, flavoring agents, natural carbohydrates, sweetening agents, vitamins, electrolytes, colorants, pectic acids, alginic acids, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents, etc. I can. In particular, as the natural carbohydrates, monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol may be used. , As the sweetener, natural sweeteners such as taumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used.

The effective dose of the extract or mixture thereof contained in the health functional food according to the present invention may be appropriately adjusted according to the purpose of use, such as skin whitening, pigmentation prevention or improvement, and wrinkle improvement. The composition has the advantage of not having side effects that may occur during long-term use of general drugs by using natural products and foods as raw materials, and has excellent portability, and can be taken as an aid for skin wrinkle improvement or whitening.

In addition, the present invention may be provided as a reagent composition for inhibiting the activity of collagenase, elastase, or tyrosinase, which contains an extract of Pseudomonas japonica extract, Gyeyo, etc. or a mixture thereof as an active ingredient.

Hereinafter, examples will be described in detail to aid understanding of the present invention. However, the following examples are merely illustrative of the contents of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely describe the present invention to those of ordinary skill in the art.

<Example 1> Fine Gaetneungja ( Atriplex gmelinii ) Extract preparation

1. Reagents and instruments

Folin &Ciocalteu's phenol reagent, dimethyl sulfoxide (DMSO), diethylene glycol, potassium peroxodisulfate, 1,1-diphenyl-2 -Picryl hydrazyl (1,1-diphenyl-2-picryl hydrazyl; hereinafter, DPPH), tannic acid, rutin, 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro- D-Arg (4-phenylazobenzyl oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg), N-succinyl-(L-Ala) ₃ -p-nitroanilide (N-succinyl-(L-Ala) ₃ -p-nitroanilide), collagenase (collagenase from Clostridium histolyticum ), elastase (elastase from porcine pancreas), tyrosine (tyrosine), and tyrosinase from mushroom are manufactured by Sigma (St. Louis, MO, USA). The product was used, and other general reagents were used with special quality products.

2. Fine-tailed snail extract

The methanol extract of Atriplex gmelinii was sold and used by the Korea Plant Extract Bank. The outposts of the slender gaetneunggo were collected on August 19, 2003 in Bomok-dong, Seogwipo-si, Jeju-do. 100 g of the pulverized sample and 1 L of 100% methanol were added to the flask and extracted for 1 hour at 45° C., and this was repeatedly extracted three times. The extract was filtered, concentrated and dried. The yield of the methanol extract of slender snail was 5.67%. Samples were dissolved in DMSO prior to use in experiments. Each of the experimental examples to be described later was measured three times and indicated the mean ± standard deviation (SD) value.

<Example 2> Gyeyo lanterns ( Paederia scandens ) Extract preparation

1. Reagents and instruments

The same reagents and equipment as in Example 1 were used.

2. Gyeyo lantern extract

The methanol extract of Paederia scandens was sold and used by the Plant Extract Bank of Korea. Outposts of Gyeyo lanterns were collected on October 13, 2004 at Odosan, Myosan-myeon, Hapcheon-gun, Gyeongsangnam-do. 100 g of the pulverized sample and 1 L of 100% methanol were added to the flask and extracted for 1 hour at 45° C., and this was repeatedly extracted three times. The extract was filtered, concentrated and dried. The yield of the methanol extract of Gyeyo etc. was 11.91%. Samples were dissolved in DMSO prior to use in experiments. Each of the experimental examples to be described later was measured three times and indicated the mean ± standard deviation (SD) value.

<Experimental Example 1> Confirmation of antioxidant activity of methanol extract (AGME) and Gyeyo etc. methanol extract (PSME)

1. Total polyphenol content and total flavonoid content

Total polyphenol content was measured by the Folin & Denis method (Folin & Denis, 1912). Add 1 mL of folin reagent to 1 mL of the sample diluted to 200 μg/mL, react at room temperature for 3 minutes _{, mix 1 mL of 10% Na 2} CO ₃ and stand at room temperature for 1 hour to measure absorbance at 725 nm I did. The calibration curve was prepared using tannic acid.

The total flavonoid content was measured by the Davis variant (Davies et al, 1980). 1 mL of diethylene glycol was mixed with 100 μL of a sample diluted to a concentration of 200 μg/mL, reacted at room temperature for 5 minutes, and then 100 μL of a 1 N NaOH solution was added and allowed to stand at 37° C. for 1 hour, and the absorbance was measured at 420 nm. The calibration curve was prepared using rutin.

1 is a graph showing the total content of polyphenols and flavonoids in a methanol extract (AGME) according to an experimental example of the present invention.

Referring to FIG. 1, the total polyphenols of the methanol extract of Psyllium chinensis were found to be 268.7 TAE (tannic acid equivalent) mg/g, and the total flavonoids were 247.5 RE (rutin equivalent) mg/g.

2 is a graph showing the total content of polyphenols and flavonoids in a methanol extract (PSME) such as Gyeyo according to an experimental example of the present invention.

Referring to FIG. 2, the total polyphenol of the methanol extract such as Gyeyo was found to be 373.3 TAE (tannic acid equivalent) mg/g, and the total flavonoid was 305.8 RE (rutin equivalent) mg/g.

2. Electronic donation ability

The electron donating ability was measured by the method of Blois (1958). Add 50 μL of 0.2 mM DPPH solution dissolved in ethanol to a 96-well plate and 100 μL of methanol extract or Gyeyo-eung methanol extract at 62.5, 125, 250, 500, and 1000 μg/mL concentrations, respectively, for 30 minutes under light-shielding condition. After standing, the absorbance was measured at 517 nm. _{SC 50} (50% scavenging concentration, 50% scavenging concentration) was calculated using GraphPrism software Ver.7 (Graphpad Software, lnc., La Jolla, CA, USA).

Figure 3 is a graph showing the electron donating ability according to the concentration of the methanol extract (AGME) according to an experimental example of the present invention.

Referring to Figure 3, the concentration-dependently of the methanol extract showed a positive (+) dose-response correlation, ascorbic acid (AA) as a positive control and electron donating ability for the methanol extract. Was found to be 97.2 and 83.2% at 1000 μg/mL, respectively.

SC After a calculated _50, going gaetneung ridden methanol extracts from the SC ₅₀ Although not the other hand, the positive control group of ascorbic acid is ₅₀ SC value calculated by 249.0μg / mL calculated was found to be lower than 62.5μg / mL.

Figure 4 is a graph showing the electron donating ability according to the concentration of the methanol extract (PSME) such as gyeyo according to an experimental example of the present invention.

Referring to FIG. 4, methanol extracts such as Gyeyo showed a positive (+) dose-response correlation in a concentration-dependent manner, and electron donating abilities for methanol extracts such as ascorbic acid and Gyeyo, which are positive controls, were 97.2 and 91.5% at 1000 μg/mL, respectively. Appeared as.

After calculating the SC _50, SC ₅₀ paederia foetida methanol extracts are Although not the other hand, the positive control group of ascorbic acid is ₅₀ SC value calculated by 149.2μg / mL calculated was found to be lower than 62.5μg / mL.

<Experimental Example 2> Enzymatic activity confirmation of methanol extract (AGME) and Gyeyo etc. methanol extract (PSME)

1. Collagenase activity

4-phenylazobenzyloxycarbonyl in 0.1M Tris buffer solution (Tris-HCl buffer, pH 7.5) added with 100 μL of sample diluted to 62.5, 125, 250, 500, 1000 μg/mL and 4 mM CaCl ₂ After mixing 250 μL of a substrate solution in which -Pro-Leu-Gly-Pro-D-Arg (0.3 mg/mL) was dissolved, 150 μL of collagenase (0.2 mg/mL) was added and reacted at room temperature for 20 minutes. Here, 1 mL of 6% citric acid was mixed, and 1.5 mL of ethyl acetate was mixed to stop the reaction. After that, the supernatant was taken and absorbance was measured at 320 nm. Ascorbic acid (AA) was used as a positive control. _{The IC 50} (50% inhibitory concentration, 50% inhibitory concentration of collagenase activity) was calculated using GraphPrism software.

5 is a graph showing the inhibitory ability of collagenase activity according to the concentration of a methanol extract (AGME) according to an experimental example of the present invention.

Referring to Figure 5, the concentration-dependently of the methanol extract showed a positive (+) dose-response correlation, and the inhibitory ability of collagenase activity against the positive control, ascorbic acid and the methanol extract, was 1000 μg/mL. In 69.7 and 41.4%, respectively.

In addition, the IC ₅₀ of ascorbic acid was calculated to be 448.3 μg/mL, and the IC ₅₀ of the methanol extract was not calculated, but it was found to be greater than 1000 μg/mL.

6 is a graph showing the inhibitory ability of collagenase activity according to the concentration of a methanol extract (PSME) such as Gyeyo according to an experimental example of the present invention.

Referring to Figure 6, the methanol extracts such as Gyeyo showed a positive (+) dose-response correlation in a concentration-dependent manner, and the inhibitory ability of collagenase activity for methanol extracts such as ascorbic acid and Gyeyo, which were positive controls, was 69.7 at 1000 μg/mL, respectively, It was found to be 62.8%.

_{In addition, IC 50} of methanol extracts such as ascorbic acid and Gyeyo were calculated to be 448.3 and 697.9 μg/mL, respectively.

2. Elastase activity

62.5, 125, 250, 500, 1000μg / mL of a sample diluted to a concentration of 5μL and 0.2 unit of porcine pancrease elastase dissolved in 100mM Tris-HCl buffer (pH 8) 5μL and N-succinyl-( L-Ala) ₃ -p-nitroanilide (0.5mg/mL) was added to 20 μL of a substrate solution and reacted at 37° C. for 10 minutes. After cooling for 5 minutes on ice, the absorbance was measured at 410 nm. Ascorbic acid was used as a positive control. _{IC 50} (50% inhibitory concentration, 50% inhibitory concentration of elastase activity) was calculated using GraphPrism software.

7 is a graph showing the inhibitory ability of elastase activity according to the concentration of the methanol extract (AGME) of the present invention according to an experimental example.

Referring to FIG. 7, the methanol extract showed a positive (+) dose-response correlation in a concentration-dependent manner, and the inhibitory activity of elastase activity against the positive control, ascorbic acid and the methanol extract, was 1000 μg/mL. In 40.5 and 36.9%, respectively. _{The IC 50} of the methanol extract of ascorbic acid and chinensis was not calculated, but it was found to be greater than 1000 μg/mL.

8 is a graph showing the inhibitory ability of elastase activity according to the concentration of a methanol extract (PSME) such as Gyeyo according to an experimental example of the present invention.

Referring to FIG. 8, methanol extracts such as Gyeyo showed a positive (+) dose-response correlation in a concentration-dependent manner, and the inhibitory activity of elastase activity against methanol extracts such as ascorbic acid and Gyeyo, which were positive controls, was 40.5 at 1000 μg/mL, respectively. It was found to be 56.0%.

In addition, the IC ₅₀ of the methanol extract of Gyeyo was calculated as 824.8 μg/mL, and the IC ₅₀ of ascorbic acid was not calculated, but it was found to be greater than 1000 μg/mL, so that the methanol extract of Gyeyo etc. was more effective in inhibiting elastase activity than ascorbic acid, a positive control. It was confirmed that this is high.

3. Mushroom tyrosinase activity

Add 50 μL of sodium phosphate buffer (pH 6.8), 50 μL of 0.03% tyrosine, and 10 μL of mushroom tyrosinase to 5 μL of the sample diluted to 62.5, 125, 250, 500, 1000 μg/mL concentration, and react at 37°C for 10 minutes and ice After cooling for 5 minutes at, the absorbance was measured at 475 nm. Arbutin was used as a positive control. _{The IC 50} (50% inhibitory concentration, 50% inhibitory concentration of tyrosinase activity) was calculated using GraphPrism software.

9 is a graph showing the ability to inhibit the tyrosinase activity according to the concentration of the methanol extract (AGME) of the tyrosinase according to an experimental example of the present invention.

Referring to Figure 9, the methanol extract concentration-dependently showed a positive (+) dose-response correlation, and the inhibitory ability of tyrosinase activity against the positive control, arbutin and methanol extract, was at a concentration of 1000 μg/mL. It was found to be 73.1 and 74.7%, respectively.

In addition, the results obtained by calculating the IC _50, IC ₅₀ of the Arbutin positive control was calculated by 175.3μg / mL, though thin IC ₅₀ of gaetneung ridden methanol extract is not calculated, it is found to be smaller than 62.5μg / mL, thin gaetneung It was confirmed that the methanol extract of chinensis was higher in whitening efficacy than the positive control, arbutin.

10 is a graph showing the inhibitory ability of tyrosinase activity according to the concentration of a methanol extract (PSME) such as Gyeyo according to an experimental example of the present invention.

Referring to FIG. 10, the methanol extract of Gyeyo showed a positive (+) dose-response correlation in a concentration-dependent manner, and the inhibitory ability of tyrosinase activity against the positive control, arbutin, and the methanol extract of Gyeyo, was 73.1 and 65.5 at 1000 μg/mL concentration, respectively. %.

In addition, IC ₅₀ of the result, the arbutin, and the methanol extract paederia foetida positive control calculate the IC ₅₀ was determined for each 175.3, 126.3μg / mL is calculated by, the whitening effect is higher than in the methanol extract paederia foetida positive control arbutin.

As described above, a specific part of the present invention has been described in detail, and for those of ordinary skill in the art, it is obvious that this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby. Do. That is, the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (7)

Thin gaetneung ridden (Atriplex gmelinii) cosmetic composition for skin whitening comprising methanol extract as an active ingredient. The method of claim 1,
The composition,
Gyeyo-deung (Paederia scandens) a cosmetic composition for skin whitening, characterized in that it further comprises a methanol extract. The method according to claim 1 or 2,
The extract,
A cosmetic composition for skin whitening, characterized in that it inhibits the activity of tyrosinase. The method according to claim 1 or 2,
The extract,
A cosmetic composition for skin whitening, characterized in that it is contained in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total cosmetic composition. The method according to claim 1 or 2,
The composition,
A cosmetic composition for skin whitening, characterized in that it has a formulation of a flexible lotion, a nutritional lotion, a nutritional cream, an essence or a pack. Gaetneung going ridden (Atriplex gmelinii) for skin containing methanol extract as an active ingredient whitening functional food composition. The method of claim 6,
The composition,
Gyeyo-deung (Paederia scandens) health functional food composition for skin whitening, characterized in that it further comprises a methanol extract.

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