KR102214557B1 - Cosmetic composition comprising dystaenia takeshimana extract - Google Patents

Abstract

The present invention relates to a cosmetic and health functional food composition comprising an island body ( Dystaenia takeshimana ) extract as an active ingredient, and more particularly, a cosmetic composition for improving skin wrinkles, a cosmetic composition for skin whitening, and It provides a health functional food composition.
The composition has an antioxidant ability, and can exhibit excellent wrinkle improvement effect and skin whitening effect by inhibiting the activity of collagenase, elastase, and tyrosinase, it can be used as a functional cosmetic composition and health functional food composition.

Description

Cosmetic composition containing island body extract as an active ingredient {COSMETIC COMPOSITION COMPRISING DYSTAENIA TAKESHIMANA EXTRACT}

The present invention relates to a cosmetic composition and a health functional food composition containing an island body ( Dystaenia takeshimana ) extract as an active ingredient.

Skin aging is a phenomenon that occurs through functional, structural, and biochemical processes in both body tissues and cells, and modern people seeking a healthy and relaxing life have increased interest in skin health, resulting in functional cosmetics derived from natural extracts for wrinkle improvement and whitening. There is a trend of increasing preference for. In response to this, research and development on functional foods and functional cosmetic materials made of natural materials are actively progressing.

Reactive oxygen species (ROS), one of the causes of skin aging, are substances produced by various factors such as energy generation process in the body and exposure to ultraviolet rays, and fragmentation of DNA, peroxidation and inactivation of lipids and proteins, etc. It causes aging by reducing the biological function. Due to this ROS, human skin exhibits several functional and structural changes. Changes in the structure of the stratum corneum of the skin, intrinsic aging, which decreases the biosynthesis of biological proteins, as well as extrinsic aging caused by exposure to various contaminants and UV rays, reduces skin elasticity, wrinkles, blemishes, Freckles, etc. are produced.

The occurrence of skin wrinkles is a representative symptom of aging skin. The main cause is a remarkable decrease in collagen, a collagen protein produced from fibroblasts, and elastin, a elastic protein protein. Collagen is a major component of the extracellular matrix (ECM), which accounts for about 80% of the skin dermis. It forms most of the organic substances in the skin, tendons, bones and teeth. It serves to protect the body. Elastin, an insoluble fiber protein that contributes to elasticity, accounts for 3-4% of the dermal layer and surrounds the collagen bundle. As skin aging progresses, the number and function of skin fibroblasts decrease, resulting in a decrease in the production of matrix proteins such as collagen and elastin, and the activities of collagenase and elastase that degrade them increase. Elasticity, moisture, and smoothness are slowed down.

Melanin (melanin) is widely distributed in living organisms, and is one of the major factors determining the color of human skin, hair, and eyes. Melanin is synthesized from melanocytes existing in the epidermal layer of the skin and transferred to keratinocytes through dendrite to protect the skin from harmful elements such as ultraviolet rays. Melanin production, a major factor causing abnormal skin pigmentation problems, is produced by enzymes such as tyrosinase, tyrosinase related protein-1 (TRP-1), and TRP-2. Therefore, by inhibiting the activity or synthesis of tyrosinase, melanin production can be reduced, and abnormal skin pigmentation problems such as melasma and freckles can be suppressed.

Island body ( Dystaenia takeshimana ) is a perennial herb belonging to the Umbelliferae family, and is also called'pigweed ', and is a Korean specialty plant that grows natively in Jeollanam-do, Chungnam, Gyeonggi-do and mainly Ulleungdo. Island body is a wild vegetable that is still enjoyed on Ulleungdo. It is effective in detoxification, mountain breeze, blue heat, and sodam. Studies on anti-inflammatory activity have been reported so far, but there are no reports on skin wrinkle improvement or whitening effect. to be.

Korean Patent Publication No. 10-2019-0058102 (published on May 29, 2019)

In order to solve the above problems, the present invention provides a cosmetic composition made of a natural material having excellent effects on skin wrinkle improvement and skin whitening.

In addition, the present invention provides a health functional food composition made of natural materials having excellent effects on skin wrinkle improvement and skin whitening.

The present invention provides a cosmetic composition for improving skin wrinkles containing an island body ( Dystaenia takeshimana ) extract as an active ingredient.

The present invention provides a cosmetic composition for skin whitening containing an island body ( Dystaenia takeshimana ) extract as an active ingredient.

The present invention provides a health functional food composition containing the island body ( Dystaenia takeshimana ) extract as an active ingredient.

The island body ( Dystaenia takeshimana ) extract according to the present invention has excellent antioxidant activity, is effective in improving skin wrinkles by inhibiting the activity of collagenase and elastase, and has effective properties in preventing or improving pigmentation by inhibiting tyrosinase activity. .

Accordingly, cosmetic compositions and health functional food compositions containing the extract as an active ingredient exhibit excellent effects on skin wrinkle improvement or skin whitening, and thus can be usefully used as functional cosmetics or health functional foods.

In addition, the composition has few side effects and can be safely used by using natural products.

1 is a graph showing the total content of polyphenols and flavonoids of the island body methanol extract (DTME) according to an experimental example of the present invention.
2 is a graph showing the electron donating ability according to the concentration of the island body methanol extract (DTME) according to an experimental example of the present invention.
3 is a graph showing the inhibitory ability of collagenase activity according to the concentration of the island body methanol extract (DTME) according to an experimental example of the present invention.
4 is a graph showing the inhibitory ability of elastase activity according to the concentration of the island body methanol extract (DTME) according to an experimental example of the present invention.
5 is a graph showing the ability to inhibit tyrosinase activity according to the concentration of the island body methanol extract (DTME) according to an experimental example of the present invention.

Hereinafter, the present invention will be described in detail.

The present inventors selected the island body as a new natural product material that requires higher safety and functionality than the existing natural plant, and the present invention by confirming the possibility of application as a functional cosmetic natural product material through various physiologically active features found in the methanol extract of the island body. Was completed.

In the present invention, the term "extract" refers to a substance obtained by extracting a component of a natural substance regardless of the extraction method, extraction solvent, extracted component, or form of the extract, and after extracting the substance obtained by extracting the component of a natural substance Any material that can be obtained by processing or processing by a method may be included.

In the present invention, the term "cosmetics" may include functional cosmetics having an effect of preventing or improving skin aging, and the like, and the functional cosmetics, unlike general cosmetics, have a physiologically active effect and a professional functionality in which the effect is emphasized. , As defined in the Cosmetics Act, it refers to a cosmetic that emphasizes specific effects and effects such as skin whitening and skin wrinkle improvement.

In the present invention, the term "health functional food" is a food manufactured and processed using raw materials or ingredients having useful functions for the human body, and is a food with high medical and medical effects processed so that the biological control function is effectively displayed in addition to nutrition supply It means and can be mixed with terms known in the art such as functional food.

The present invention provides a cosmetic composition for improving skin wrinkles containing an island body ( Dystaenia takeshimana ) extract as an active ingredient.

In the cosmetic composition according to the present invention, the island body extract may be extracted using a C1 to C4 alcohol or an aqueous solution thereof as a solvent, and preferably extracted using methanol or an aqueous methanol solution as a solvent. The island body may be harvested or cultivated in nature, and may be purchased commercially. The island body extract may be obtained by extracting the flowers, leaves, stems, roots, or a mixture of these parts of the island body, and preferably extracting the root of the island body.

The island body extract may be extracted according to a method commonly used in the art, for example, a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, etc., and these are performed alone or in combination of two or more methods. Can be done.

In the present invention, "wrinkle improvement" means inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating wrinkles that have already been created. Wrinkle generation is mainly caused by a decrease in collagen, and the decrease in collagen may be caused by excessive oxidative stress or ultraviolet irradiation.

The cosmetic composition according to the present invention may have antioxidant activity, collagenase activity inhibitory activity, or elastase activity inhibitory activity. The collagenase is a collagen-degrading enzyme, which inhibits and decomposes the synthesis of collagen constituting the skin tissue, thereby weakening skin elasticity and generating wrinkles. The elastase is an elastin degrading enzyme, and, like the collagenase, is a major cause of skin elasticity reduction and wrinkle formation.

The cosmetic composition according to the present invention may exhibit excellent skin wrinkle improvement effects by inhibiting collagenase or elastase activity. The anti-wrinkle effect may include conventional skin protection effects such as preventing, improving, and inhibiting or improving skin aging. In addition, since collagen and elastin are involved in the mechanical firmness of the skin, the binding force and elasticity of the tissue, the cosmetic composition can improve skin elasticity, skin regeneration, wound healing, and repair and regeneration of damaged skin tissue.

According to an experimental example of the present invention, the methanol extract of the island body was found to contain 383.9 TAE mg/g of polyphenol and 275.8 RE mg/g of flavonoids. The polyphenols and flavonoids are secondary metabolites of plants, and are antioxidants that can increase the safety of foods or cosmetics by inhibiting antioxidant activity and lipid peroxidation. In addition, electron donating ability through DPPH radical scavenging ability of the island body methanol extract and its positive control, ascorbic acid, showed similar values of 96.1 and 97.2% at 1000 μg/mL, respectively.

According to another experimental example of the present invention, the inhibitory activity of collagenase activity of the methanol extract of the island body and the positive control ascorbic acid was 65.0 and 69.7%, respectively, at 1000 μg/mL, and the IC ₅₀ was calculated as 496.4 and 448.3 μg/mL, respectively. .

In addition, the inhibitory ability of the island body methanol extract and the positive control ascorbic acid to inhibit elastase activity was 60.3 and 40.5% at 1000 μg/mL, respectively, and the IC ₅₀ of the island body methanol extract was calculated as 758.0 μg/mL, whereas the IC of ascorbic acid _{Although 50} was not calculated, it was confirmed that it was greater than 1000 μg/mL, and it was confirmed that the islandbody methanol extract had a similar or superior level of collagenase or elastase activity inhibitory activity than its positive control. More details will be described later in the following experimental examples.

The present invention provides a cosmetic composition for skin whitening containing an extract of Dystaenia takeshimana as an active ingredient.

In the cosmetic composition according to the present invention, the island body extract may be extracted using a C1 to C4 alcohol or an aqueous solution thereof as a solvent, and preferably extracted using methanol or an aqueous methanol solution as a solvent. The island body may be harvested or cultivated in nature, and may be purchased commercially. The island body extract may be obtained by extracting the flowers, leaves, stems, roots, or a mixture of these parts of the island body, and preferably extracting the root of the island body.

In the present invention, "whitening" refers to an action of suppressing, inhibiting, or alleviating excessive deposition of melanin pigments and the like to prevent blackening of the skin caused by the pigmentation, thereby improving skin problems.

The cosmetic composition according to the present invention may have a tyrosinase activity inhibitory ability. The tyrosinase is an enzyme involved in the melanin production process that induces pigmentation, and the islandbody extract may exhibit excellent skin whitening effect by inhibiting the activity of the tyrosinase. The whitening effect may include freckles, melasma, hyperpigmentation after UV exposure, hyperpigmentation after inflammation, prevention and improvement of pigmentation including senile black spots, brown spots or age spots.

According to an experimental example of the present invention, the tyrosinase activity inhibitory ability of the island body methanol extract and its positive control arbutin was 71.0 and 73.1% at 1000 μg/mL, respectively, and IC ₅₀ was calculated as 138.3 and 175.3 μg/mL, respectively, and the island body It can be seen that the methanol extract has excellent tyrosinase activity inhibition.

In the cosmetic composition according to the present invention, the island body extract may be included in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total cosmetic composition, but is not limited thereto.

The cosmetic composition according to the present invention may further include one or more of the ingredients registered in the list of cosmetic ingredients registered with the Food and Drug Administration and the ICID (International Cosmetic Material Collection). For example, the cosmetic composition is an organic solvent, a solubilizer, a thickener, a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, a fragrance, a surfactant, an emulsifier, a filler, a sequestering agent, a preservative, a vitamin, a blocker, It may further comprise one or more adjuvants commonly used in the cosmetic field, such as wetting agents, dyes and pigments, hydrophilic or lipophilic actives, or any other ingredients commonly used in cosmetics.

The vitamin may include a water-soluble or oil-soluble vitamin, and the water-soluble vitamin is a water-soluble vitamin that can be blended in cosmetics, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, Nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like, and more preferably, vitamin C, which helps collagen synthesis, may be included. In addition, their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-magnesium salt, etc.) may also be included, and the water-soluble vitamin is a microbial transformation method. , It can be obtained by a conventional method such as a purification method from a culture of microorganisms, an enzyme method, or a chemical synthesis method.

The cosmetic composition according to the present invention can be prepared in any formulation conventionally prepared in the technical field to which the present invention belongs. For example, it may be formulated as a lotion, emulsion, lotion, cream, paste, gel, solution, suspension, oil, wax, pack, powder, foundation, spray, surfactant-containing cleansing, etc., but is not limited thereto. . More specifically, skin lotion, skin softener, skin toner, nutrition cream, massage cream, milk lotion, powder, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, powder foundation, emulsion foundation , Cleansing cream, cleansing foam, cleansing water, soap, pack, stick-like product, balm type product, spray or powder formulation.

When the cosmetic composition according to the present invention is in a cream or gel formulation, it may further include animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component. I can.

When the cosmetic composition is a solution or emulsion formulation, as a solvent, solvating agent or emulsifying agent, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or fatty acid of sorbitan It may further include esters and the like.

When the cosmetic composition is a suspension formulation, as a carrier component, liquid diluents such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline It may further include cellulose, aluminum metahydroxide, bentonite, agar, or tracant.

When the cosmetic composition is a powder or spray formulation, it may further include lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder as a carrier component, and in particular, in the case of a spray formulation, additional chlorofluorohydrocarbon , It may further include a propellant such as propane / butane or dimethyl ether.

When the cosmetic composition is a surfactant-containing cleansing formulation, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester, and the like may be further included.

The cosmetic composition according to the present invention may be used alone or in duplicate, or may be used in duplicate with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use thereof may be varied according to a user's skin condition or taste.

The present invention provides a health functional food composition containing the island body ( Dystaenia takeshimana ) extract as an active ingredient.

The composition may have an antioxidant activity, a collagenase activity inhibitory activity, an elastase activity inhibitory activity, and a tyrosinase activity inhibitory activity, and thus may exhibit a skin wrinkle improvement or skin whitening effect. The anti-wrinkle effect may include conventional skin protective effects such as preventing and improving wrinkles, inhibiting and improving skin aging, and collagen and elastin are involved in the mechanical firmness of the skin, the binding force and elasticity of the tissue, etc. It is possible to obtain effects such as regeneration, wound healing, and repair and regeneration of damaged skin tissue. In addition, the whitening effect may include freckles, melasma, hyperpigmentation after exposure to ultraviolet rays, hyperpigmentation after inflammation, prevention of pigmentation including black spots, brown spots, age spots, and the like.

In the health functional food composition according to the present invention, the health functional food may be prepared as a powder, granule, tablet, capsule, syrup or beverage, and there is no limitation on the form that the health functional food can take. It can include all foods of meaning. For example, beverages and various drinks, fruits and processed foods thereof (canned fruit, jam, etc.), fish, meat and processed foods thereof (ham, bacon, etc.), bread and noodles, cookies and snacks, dairy products (butter, cheese, etc.) ), and the like, and may include all functional foods in the usual sense. It may also include food used as feed for animals.

The health functional food composition according to the present invention may be prepared further comprising a food pharmaceutically acceptable food additive and other appropriate auxiliary ingredients commonly used in the art. For example, flavoring agents, natural carbohydrates, sweetening agents, vitamins, electrolytes, colorants, pectic acids, alginic acids, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents, etc. I can. In particular, as the natural carbohydrates, monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol may be used. , As the sweetener, natural sweeteners such as taumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used.

The effective dose of the island body extract contained in the health functional food according to the present invention may be appropriately adjusted according to the purpose of use, such as prevention or improvement of skin wrinkles or pigment coloring. The composition has the advantage of not having side effects that may occur during long-term use of general drugs by using natural products and foods as raw materials, and has excellent portability, and can be taken as an adjuvant for skin wrinkle improvement or skin whitening.

In addition, the present invention can provide a reagent composition for inhibiting the activity of collagenase, elastase, or tyrosinase containing the island body extract as an active ingredient.

Hereinafter, examples will be described in detail to aid understanding of the present invention. However, the following examples are for illustrative purposes only, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

<Example 1> Preparation of island body extract

1. Reagents and instruments

Folin &Ciocalteu's phenol reagent, dimethyl sulfoxide (DMSO), diethylene glycol, sodium phosphate, 1,1-diphenyl-2- Picryl hydrazyl (1,1-diphenyl-2-picryl hydrazyl; hereinafter, DPPH), tannic acid, rutin, 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D -Arg (4-phenylazobenzyloxycarbonyl-Pro- Leu-Gly-Pro-D-Arg), N- succinyl - (L-Ala) ₃ -p- not nitro fluoride (N-succinyl- (L-Ala ) 3 -p -nitroanilide), collagenase from Clostridium histolyticum, elastase from porcine pancreas, tyrosine, and mushroom tyrosinase from Sigma. A product of St. Louis, MO, USA was used, and other general reagents were used with special quality products.

2. Island Body Extract

Island body ( D. takeshimana ) methanol extract was used by pre-sale from Korea Plant Extract Bank. Island body roots were collected in October 2005 in the Nari Basin, Buk-myeon of Ulleungdo. 100 g of the pulverized sample and 1 L of 100% methanol were added to the flask and extracted for 1 hour at 45° C., which was repeatedly extracted three times. The extract was filtered, concentrated and dried. The yield of the methanol extract of the island body was 7.58%. The sample was dissolved in DMSO, diluted from 1000 μg/mL to 62.5 μg/mL with distilled water, and then used in the experiment. Each of the experimental examples to be described later was measured three times and indicated the mean ± standard deviation (SD) value.

<Experimental Example 1> Confirmation of antioxidant activity of the island body methanol extract (DTME)

1. Total polyphenol content and total flavonoid content

The total polyphenol content was measured by the Folin & Denis method (Folin & Denis, 1912). To 1 mL of the sample diluted to 200 μg/mL, add 1 mL of folin reagent, react at room temperature for 3 minutes, mix 1 mL of 10% Na ₂ CO ₃ and stand at room temperature for 1 hour to measure absorbance at 725 nm I did. The calibration curve was prepared using tannic acid.

The total flavonoid content was measured by the Davis variant (Davies et al, 1980). 1 mL of diethylene glycol was mixed with 100 μL of a sample diluted to a concentration of 200 μg/mL, reacted at room temperature for 5 minutes, and then 100 μL of a 1 N NaOH solution was added and allowed to stand at 37° C. for 1 hour, and the absorbance was measured at 420 nm. The calibration curve was prepared using rutin.

1 is a graph showing the total content of polyphenols and flavonoids of the island body methanol extract (DTME) according to an experimental example of the present invention.

Referring to FIG. 1, the total polyphenol of the island body methanol extract (DTME) was 383.9 TAE (tannic acid equivalent) mg/g, and the total flavonoids were 275.8 RE (rutin equivalent) mg/g.

2. Electronic donation ability

The electron donating ability was measured by the method of Perez (Perez et al. 2004). To a 96-well plate, 50 μL of 0.2 mM DPPH solution dissolved in ethanol and 100 μL of island body methanol extract at concentrations of 62.5, 125, 250, 500, and 1000 μg/mL were added each, and left for 30 minutes in a light-shielding state, and then at 517 nm. The absorbance was measured. SC ₅₀ (50% scavenging concentration, 50% free radical scavenging concentration) was calculated using GraphPrism software Ver.7 (Graphpad Software, lnc., La Jolla, CA, USA).

2 is a graph showing the electron donating ability according to the concentration of the island body methanol extract (DTME) according to an experimental example of the present invention.

2, the island body methanol extract showed a positive (+) dose-response correlation in a concentration-dependent manner, and the electron donating ability for the positive control ascorbic acid (AA) and the island body methanol extract was 1000 μg/mL. In 97.2 and 96.1%, respectively.

After calculating the SC _50, SC ₅₀ seombadi methanol extracts are Although not the other hand, the positive control group of ascorbic acid is ₅₀ SC value calculated by 257.4μg / mL calculated was found to be lower than 62.5μg / mL.

<Experimental Example 2> Enzyme activity confirmation of the island body methanol extract (DTME)

1. Collagenase activity

4-phenylazobenzyloxycarbonyl in 0.1M Tris-HCl buffer (pH 7.5) containing 100 μL of a sample diluted to 62.5, 125, 250, 500, 1000 μg/mL and 4 mM CaCl ₂ After mixing 250 μL of a substrate solution in which -Pro-Leu-Gly-Pro-D-Arg (0.3 mg/mL) was dissolved, 150 μL of collagenase (0.2 mg/mL) was added and reacted at room temperature for 20 minutes. Here, 1 mL of 6% citric acid was mixed, and 1.5 mL of ethyl acetate was mixed to stop the reaction. Then, the supernatant was taken and absorbance was measured at 320 nm. Ascorbic acid (AA) was used as a positive control. IC ₅₀ (50% inhibitory concentration, 50% inhibitory concentration of collagenase activity) was calculated using GraphPrism software.

3 is a graph showing the inhibitory ability of collagenase activity according to the concentration of the island body methanol extract (DTME) according to an experimental example of the present invention.

3, the island body methanol extract showed a positive (+) dose-response correlation in a concentration-dependent manner, and the inhibitory activity of collagenase activity for the positive control ascorbic acid and the methanol extract of the island body was 69.7 at 1000 μg/mL, respectively, It was found to be 65.0%.

In addition, IC ₅₀ of the methanol extract of ascorbic acid and island body was calculated to be 448.3 and 496.4 μg/mL, respectively.

2. Elastase activity

62.5, 125, 250, 500, 1000μg / mL of a sample diluted to a concentration of 5μL of 0.2 unit of porcine pancrease elastase dissolved in 100mM Tris-HCl buffer (pH 8) 5μL and N-succinyl-( L-Ala) ₃ -p-nitroanilide (0.5mg/mL) was added to 20 μL of a substrate solution and reacted at 37° C. for 10 minutes. After cooling for 5 minutes on ice, the absorbance was measured at 410 nm. Ascorbic acid was used as a positive control. IC ₅₀ (50% inhibitory concentration, 50% inhibitory concentration of elastase activity) was calculated using GraphPrism software.

4 is a graph showing the inhibitory ability of elastase activity according to the concentration of the island body methanol extract (DTME) according to an experimental example of the present invention.

4, the island body methanol extract showed a positive (+) dose-response correlation in a concentration-dependent manner, and the inhibitory activity of elastase activity against the positive control ascorbic acid and the methanol extract of the island body was 40.5 at 1000 μg/mL, respectively, It was found to be 60.3%.

In addition, IC ₅₀ of the result, the methanol extract seombadi calculating the IC _50, while the output in 758.0μg / mL, IC ₅₀ of the ascorbic acid was found to be greater than Although not calculated 1000μg / mL.

3. Tyrosinase (Mushroom tyrosinase) activity

Add 50 μL of sodium phosphate buffer (pH 6.8), 50 μL of 0.03% tyrosine, and 10 μL of mushroom tyrosinase to 5 μL of the sample diluted to 62.5, 125, 250, 500, 1000 μg/mL concentration, and react at 37°C for 10 minutes and ice After cooling for 5 minutes at, the absorbance was measured at 475 nm. Arbutin was used as a positive control. The IC ₅₀ (50% inhibitory concentration, 50% inhibitory concentration of tyrosinase activity) was calculated using GraphPrism software.

5 is a graph showing the ability to inhibit tyrosinase activity according to the concentration of the island body methanol extract (DTME) according to an experimental example of the present invention.

5, the island body methanol extract showed a positive (+) dose-response correlation in a concentration-dependent manner, and the mushroom tyrosinase activity inhibitory ability for the positive control arbutin and the island body methanol extract was 73.1 at a concentration of 1000 μg/mL, respectively. It was found to be 71.0%.

In addition, the results obtained by calculating the IC _50, IC ₅₀ for the positive control arbutin and seombadi methanol extract were calculated respectively 175.3, 138.3μg / mL.

As described above, specific parts of the present invention have been described in detail, and for those of ordinary skill in the art, it is obvious that these specific techniques are only preferred embodiments, and the scope of the present invention is not limited thereby. Do. That is, the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (9)

Island body ( Dystaenia takeshimana ) cosmetic composition for improving skin wrinkles containing methanol extract of the root as an active ingredient. delete The method of claim 1,
The composition,
A cosmetic composition for improving skin wrinkles, characterized in that it has the ability to inhibit collagenase activity or inhibit elastase activity. Island body ( Dystaenia takeshimana ) cosmetic composition for skin whitening containing the methanol extract of the root as an active ingredient. delete The method of claim 4,
The composition,
A cosmetic composition for skin whitening, characterized in that it has an ability to inhibit tyrosinase activity. The method of claim 4,
The composition,
A cosmetic composition for skin whitening, characterized in that used for preventing or improving pigmentation. delete delete

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