KR102459512B1 - Method for preparing complex extracts of neungi, chrysanthemum, and magnolia using Cyclodextrin Glucanotransferas and cosmetic composition containing the same - Google Patents

Abstract

The present invention relates to an antioxidant, skin whitening and moisturizing cosmetic composition containing active ingredients of a complex extract of Neungyi, chrysanthemum and magnolia using CGTase (Cyclodextrin Glucanotransferase) and a method for manufacturing the same, and Neungyi, Chrysanthemum and As an active ingredient of the magnolia complex extract, it contains 0.01 to 20.0% by weight based on the total weight of the cosmetic.
The antioxidant, skin whitening and moisturizing cosmetic composition of the present invention is safe for the skin and exhibits excellent melanin production inhibition and antioxidant activity due to enzymatic activity, synergistic action by complex components of chrysanthemum and magnolia, and thus skin whitening, wrinkle improvement and elasticity The effect is excellent according to the enhancement and moisturizing power.

Description

Method for preparing complex extracts of neungi, chrysanthemum, and magnolia using Cyclodextrin Glucanotransferas and cosmetic composition containing the same

The present invention relates to a method for producing a complex extract of Neungyi, chrysanthemum and magnolia using cyclodextrin glucanotransferase (CGTase), and an antioxidant, skin whitening and moisturizing cosmetic composition containing the same as an active ingredient.

Recently, as the industry develops rapidly and the income level improves, interest in preventing or improving skin aging along with maintaining the beauty of appearance is increasing. Research and development utilizing the high functionality of natural materials is being actively carried out.

In order to delay or improve skin aging, it is necessary to use an antioxidant that can promote collagen synthesis or give cell regeneration functions or remove free radicals. Representative antioxidants include enzymes called superoxide dismutase (SOD) and vitamins There are C, vitamin E (tocopherol), flavonoids, etc., and collagen synthesis promoting substances include vitamin C, vitamin A (retinoic acid), and skin growth factor (EGF). It is not easy to apply to cosmetics with In some cases, it is known that the effects such as antioxidant action and stability are insufficient.

In the effort to delay skin aging using cosmetics, the stabilization technology of cosmetic ingredients is important in maximizing its function, and the stability of cosmetic active ingredients is improved by microencapsulation, liposome, liquid crystal method, etc.

On the other hand, cyclodextrin glucanotransferase is an enzyme that produces a cyclic cyclodextrin, and the cyclic cyclodextrin uses its structural properties to enclose various hydrophobic compounds in the hydrophobic site in the molecule to form an inclusion complex. Various studies are being conducted to increase the bioavailability by improving the solubility and stability of the encapsulated material and improving the sustained-release properties.

As a prior art related to a method for manufacturing a cosmetic composition using an enzyme, for example, in Patent Document 1, 0.01 to 10% by weight of arazyme, a proteolytic enzyme, 20 to 30% by weight of a natural herbal extract, 5 to 15% by weight of amphoteric surfactant % and 20 to 55% by weight of purified water is disclosed, and in Patent Document 2, mixed herbal medicines of Baekbuja, Baekji, Youngneunghyang, Hyangbuja, Baekbokryeong and Gamsonghyang are solvent-extracted and mixed herbal medicine. Disclosed is a mixed herbal medicine extract for skin wrinkle improvement, which is obtained by preparing a crude extract and then treating the crude extract with a carbohydrate hydrolase and a pectin hydrolase.

In addition, Patent Document 3 includes a sugar hydrolytic enzyme-treated red ginseng and Chunma extract as active ingredients, wherein the enzyme-treated red ginseng extract is a red ginseng extract obtained by glycolysis of a red ginseng water extract with a cellulase enzyme, and the enzyme-treated Chunma extract is Chunma ethanol After converting the extract into maltooligosylglucoside using water-soluble starch and CGTase (Cyclodextrin glycosyltransferase) enzyme, it is a chonma extract that is hydrolyzed by sugar using a glucoamylase enzyme, and the red ginseng and cheonma extracts are 1: 9 Disclosed is a cosmetic composition for preventing or improving skin wrinkles, characterized in that it is mixed in a weight ratio of 11 to 11.

The inventor of the present invention uses cyclodextrin glucanotransferase (CGTase) to improve the production of physiologically active substances of Neungyi, Chrysanthemum and Magnolia while developing a cosmetic with high stability and excellent antioxidant, skin whitening and moisturizing effects, and the present invention was completed.

US 10-0828379 B1 US 10-1142541 B1 US 10-2139156 B1

The problem to be solved in the present invention relates to a method for producing a complex extract of Neungyi, chrysanthemum and magnolia using cyclodextrin glucanotransferase, and to providing an antioxidant, skin whitening and moisturizing cosmetic composition containing the same as an active ingredient.

More specifically, by adding Cyclodextrin glycosyltransferase (CGTase) to the complex extract of Neungyi, Chrysanthemum, and Magnolia, the active ingredient, the complex extract, is contained at a high concentration while improving the stability of the antioxidant, skin whitening and moisturizing cosmetic composition is intended to provide

As a means of solving the problems in the present invention, the method for producing a complex extract of Neungyi, Chrysanthemum and Magnolia using cyclodextrin glucanotransferase (CGTase) is a process of obtaining a complex extract of Neungi, Chrysanthemum, and Magnolia, and cyclodextrin in the complex extract By adding glucanotransferase to inclusion complex extracts of neungyi, chrysanthemum, and magnolia, which are active ingredients, it comprises a step of preparing a clathrate in which the complex extract is included.

The above-mentioned Neungi has a very strong scent, so it is also called Hyanghyang (incense mushroom). Its original name is Ungi (熊茸), and in dialect it is called Neungi (能耳). It is recorded in the Go (五洲衍文長箋散稿), and it has the effect of slowing down skin aging and purifying the blood. Neung (能) also means bear, so it seems that the mushroom, which was a bear mushroom in Korean, was called ungi or neungi in Chinese, and then settled as neungi.

Chrysanthemum contains a lot of essential oils such as cusanthenone, adenine, prine, beatin, and chrysacemin, a yellow pigment, which are useful for the human body. It has the effect of relieving the back, and has been frequently used as a herbal medicine or tea.

Magnolia is a deciduous broad-leaved arboreous tree that lives in Korea and Japan, and is mainly used as a garden tree. Its action is diverse, such as having an analgesic action, and the pharmacological action known to date is a component that lowers blood pressure, and among the volatile components, it is known that there is a local astringent effect.

One embodiment of a method for producing a complex extract of Neungyi, Chrysanthemum and Magnolia using cyclodextrin glucanotransferase (CGTase) according to the present invention is a). The first step of obtaining a complex extract of Neungi, chrysanthemum and magnolia by warming and extracting neungi, chrysanthemum and magnolia with purified water or hot spring water, b). A second step of manufacturing cyclodextrin in which the complex extract of Neungyi, Chrysanthemum, and Magnolia is encapsulated by adding dextrin containing cyclodextrin glucanotransferase to the complex extract of the first step to include the complex extract. is done

Another embodiment of the method for producing a complex extract of Neungyi, chrysanthemum and magnolia using cyclodextrin glucanotransferase (CGTase) according to the present invention is a) 1 to 10 parts by weight of Neungyi, 1 to 10 parts by weight of chrysanthemum, and 1 part by weight of magnolia 1st step of obtaining a complex extract of Neungyi, Chrysanthemum and Magnolia by warming and extracting ~10 parts by weight with hot spring water, b) Cyclodextrin glucanotransferase 0.01 ~ 1.0W/V with respect to 100 parts by weight of the complex extract of the first step % concentration of dextrin containing 1.0 to 5.0 parts by weight to include the complex extract of the first step to produce a cyclodextrin containing the complex extracts of Neungyi, Chrysanthemum and Magnolia .

The hot spring water of the present invention is preferably the hot spring water of Yuseong hot spring managed by Yuseong-gu, Daejeon, and the quality of the hot spring water is slightly alkaline with a water temperature of 31 to 35 ℃, pH 7.95, and the water quality type is Na-HCO ₃ type, E. coli, etc. It is being investigated as not being exposed to pollutants, and according to the data of the Korean Onsen Association, it contains more than average Korean hot spring ingredients, so it is announcing that it is in an optimal state for research and development of hot spring water cosmetics.

And as a means of solving another problem of the present invention, a cosmetic composition using a complex extract of Neungi, Chrysanthemum, and Magnolia using cyclodextrin glucanotransferase (CGTase) as an active ingredient is effective for the enzymatic action of cyclodextrin glucanotransferase. By using the structural characteristics of the cyclodextrin produced by

One embodiment of the cosmetic composition using the complex extract of Neungyi, Chrysanthemum, and Magnolia using cyclodextrin glucanotransferase (CGTase) according to the present invention as an active ingredient is the use of the cyclodextrin glucanotransferase (CGTase) , chrysanthemum, magnolia complex extract prepared according to the manufacturing method, cyclodextrin (cyclodextrin) containing the complex extract of chrysanthemum and magnolia as an active ingredient It consists of a cosmetic composition containing 0.01 to 20% by weight based on the total weight of the cosmetic. .

Cyclodextrin glucanotransferase, which is an enzyme that produces the cyclic cyclodextrin, is a well-known substance, and in the present invention, the ability to be an active ingredient is improved by using the structural characteristics of cyclodextrin produced by cyclodextrin glucanotransferase. By composing a cyclodextrin containing complex extracts of chrysanthemum, chrysanthemum and magnolia as an active ingredient in a cosmetic, it can be stabilized with an increase in the content of the active ingredient relative to the cosmetic, and the solubility of the active ingredient is improved.

The cosmetic composition according to the present invention is made according to a conventional method of manufacturing a cosmetic, and for example, a lotion, emulsion, cream, essence, pack, mask together with a pharmaceutically, cosmetic or dermatologically acceptable excipient. , foundation, sunscreen, BB cream, shampoo, treatment, body wash, body lotion, hand lotion or skin cleanser formulation.

The cosmetic composition of the present invention comprises cyclodextrin containing complex extracts of Neungyi, Chrysanthemum and Magnolia as an active ingredient, thereby improving the stability and solubility of the active ingredient with the effect of relatively increasing the content of the active ingredient in the cosmetic. indicate the characteristics.

In addition, it shows excellent skin whitening, aging delay and moisturizing effects because it is safe for the skin and exhibits excellent melanin production inhibition and antioxidant activity due to the synergistic action of active ingredients contained in high concentrations.

1 is a graph showing the Tyrosinase inhibitory activity of a complex extract of Neungyi, Chrysanthemum and Magnolia using CGTase (Cyclodextrin Glucanotransferase) according to the present invention;

Hereinafter, the present invention will be described in detail through specific contents, examples and test examples for carrying out the invention, but the present invention is not limited by the description below.

The method for producing a complex extract of Neungyi, Chrysanthemum and Magnolia using cyclodextrin glucanotransferase (CGTase) according to the present invention is a). 1 to 10 parts by weight of neungi, 1 to 10 parts by weight of chrysanthemum, and 1 to 10 parts by weight of magnolia by heating and extracting with purified water or hot spring water to obtain a complex extract of neungi, chrysanthemum and magnolia, b). By adding 1.0 to 5.0 parts by weight of dextrin containing cyclodextrin glucanotransferase at a concentration of 0.01 to 1.0 W/V% with respect to 100 parts by weight of the complex extract of the first process, the complex extract of the first process is included, It consists in including a second step of producing a cyclodextrin in which the complex extract of chrysanthemum and magnolia is included.

The first process according to the present invention is an extraction process for obtaining a complex extract of Neungyi, chrysanthemum, and magnolia, and extracts by combining 1 to 10 parts by weight of Neungyi, 1 to 10 parts by weight of chrysanthemum, and 1 to 10 parts by weight of magnolia, It is preferable to use a combination of chrysanthemum and magnolia in the same weight ratio.

The extraction process of the first step is 8 hours ~ 1 day, more preferably 10 hours ~ 18 hours, most preferably 12 hours ~ Extraction is performed for 16 hours, and if the extraction time is less than 12 hours, the extraction of the desired component is insufficient, and when the extraction time exceeds 1 day, there is no change in the extraction component and a problem of discoloration of the extract occurs.

The hot spring water is preferably the hot spring water of Yuseong Hot Springs managed by Yuseong-gu, Daejeon, and the quality of the hot spring water is slightly alkaline at a water temperature of 31 to 35 ℃ and pH 7.95, and the water quality type is Na-HCO ₃ , exposed to contaminants such as E. coli. It has been investigated as not being used, so it is particularly suitable as an extraction solvent for the manufacture of hot spring water cosmetics.

In the second process according to the present invention, dextrin containing cyclodextrin glucanotransferase (CGTase) at a concentration of 0.01 to 1.0 W/V% is added to the complex extract of Neungyi, chrysanthemum, and magnolia obtained in the first process to obtain cyclodextrin. It is a process of enclosing complex extracts of Neungyi, Chrysanthemum and Magnolia with cyclodextrin generated by the enzymatic action of glucanotransferase (CGTase).

The second process consists of reacting for 5 hours to 24 hours at a reaction condition of a temperature of 30 to 80° C. and pH 3.0 to 8.0, preferably 8 hours to a reaction condition of a temperature of 40 to 60° C. and pH 4.5 to 6.5. The reaction is carried out for 12 hours, and if the reaction conditions are not within the above range, stability and inclusion of the desired component are insufficient.

In addition, the dextrin, which is a hydrolyzate of starch added in the second step, consists of selecting DE 1 to DE 60 dextrin, preferably DE 5 to DE 30 dextrin, more preferably DE 7 to DE 12 dextrin. done by doing

The reaction product generated from the second process is concentrated under reduced pressure and dried by a method such as freeze drying, spray drying, cold air drying or hot air drying to prepare a complex extract of neungyi, chrysanthemum and magnolia in the form of a dried product as an active ingredient in cosmetics. It is advisable to prepare

And the cosmetic composition containing the complex extract of Neungyi, chrysanthemum, and magnolia using cyclodextrin glucanotransferase according to the present invention includes the complex extract of Neungi, chrysanthemum and magnolia prepared by the first to second processes described above. It consists of containing cyclodextrin as an active ingredient.

Cyclodextrin containing the compound extract of Neungi, Chrysanthemum and Magnolia as the active ingredient is contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition, preferably 0.1 to 10% by weight, and 1 to It is more preferable to contain it at 5 weight%.

If the active ingredient is less than 0.01% by weight of the total weight of the cosmetic composition, it is difficult to provide satisfactory efficacy, and if it is more than 20% by weight, it affects the odor and color of the composition, so that it is difficult to expect commercial properties and additional efficacy.

The cosmetic composition containing the complex extract of Neungyi, Chrysanthemum, and Magnolia using cyclodextrin glucanotransferase according to the present invention can be applied to a cosmetic composition for antioxidant, skin whitening or moisturizing.

In addition, the cosmetic composition according to the present invention is prepared according to a conventional method of manufacturing a cosmetic, for example, a lotion, emulsion, cream, essence, pack, mask together with pharmaceutically, cosmetically or dermatologically acceptable excipients. , foundation, sunscreen, BB cream, shampoo, treatment, body wash, body lotion, hand lotion or skin cleanser formulation.

Hereinafter, the present invention will be described in more detail through <Examples> and <Test Examples>.

<Example 1>

1. Preparation of complex extracts of Neungyi, Chrysanthemum and Magnolia

500 g of commercially available Neungyi, 500 g of chrysanthemum, and 500 g of magnolia were finely cut with a grinder in a mixed state, put into a prepared extractor, 15,000 g of oily hot spring water was added, and then extracted at 50° C. for 12 hours. After the extraction was completed, the solid was removed and a filtrate was obtained.

15,000 g of the obtained filtrate, 10 g of maltodextrin (DE 12), and 1 g of CGTase were added to the prepared reaction vessel, and after 8 hours of reaction at 50° C., pH 5.5, the enzyme was inactivated by heating at 90° C. for 30 minutes.

After the reaction was completed, the filtrate was concentrated under reduced pressure and freeze-dried to obtain 12.5 g of dry powder of a clathrate (enzyme complex extract) containing complex extracts of Neungyi, Chrysanthemum and Magnolia.

<Comparative Example 1>

For comparison, 500 g each of Neungyi, Chrysanthemum and Magnolia were mixed and pulverized, and the complex extract extracted under the same extraction conditions as in <Example 1> was dried to obtain 4.7 g of dry powder.

<Test Example 1> Polyphenol content measurement test

The polyphenol content was measured using each of the samples prepared in <Example 1> and <Comparative Example 1>.

To measure the total polyphenol content, after diluting the extract sample obtained by each method to 5 mg/ml, 25ul of the sample was put in 48well, 50ul of Folin & ciocaltean was added, reacted for 3 minutes, and then 1ml of 2% sodium carbonate was added After mixing, absorbance was measured at 750 nm and shown in [Table 1] below.

Sample name Total polyphenol content (mg GA eq/g) Example 1 Enzyme Complex Extract 101.17±1.56 Comparative Example 1 simple complex extract 69.81±2.31

As shown in [Table 1], it can be confirmed that the complex extract of chrysanthemum and magnolia using the CGTase enzyme of <Example 1> has a higher polyphenol content than the simple complex extract of <Comparative Example 1>.

<Test Example 2> Skin cell toxicity test

Toxicity to skin cells was measured using each of the samples prepared in <Example 1> and <Comparative Example 1>.

The cell line used for this test was 3 types of cells including human dermal fibroblast (Lonza, Basel, Switzerland). The cells were prepared in Dulbecco's Modified Eagle Medium (DMEM; Gibco-BRL, Invitrogen; Life Technologies, Gaithersburg, MD, USA) in 10% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), 1% Using a medium containing penicillin / streptomycin (penicillin 100 IU / ml, streptomycin 100 μg / ml; Life Technologies), 5% CO ₂ In a cell incubator, cultured under a temperature condition of 37 ° C.

The WST-1 (Water-soluble tetrazolium salt) assay is a method to measure and investigate the activity of mitochondrial NADH-dehydrogenase in cells. Then, the colored formazan is formed by the dehydrogenase in the mitochondria. If the formed formazan is confirmed by the absorbance at a wavelength of 450 nm, the concentration of living cells can be confirmed. Cells were seeded in a 96-well plate at 2×10 ³ cells/well and cultured for 24 hours. After that, the WST-1 assay solution was treated by 10% of the amount of the medium and reacted at 37°C for additional 0.5 to 1 hour, and then absorbance at 450 nm using an iMark microplate reader (Bio-Rad, Hercules, CA, USA). was measured and the reference absorbance was measured at 650 nm to correct the result. The results were expressed as mean ± standard deviation from three independent experiments, and are shown in [Table 2] below. P<0.05 was verified through Student's t-test and indicates the significance of the results.

Sample name Concentration (ppm) Cell viability (%) Example 1 Enzyme Complex Extract 100 101.5 150 104.2 200 110.0 Comparative Example 1 simple compound
extract 100 100.9 150 102.8 200 108.9

As shown in [Table 2], it can be seen that the complex extract of chrysanthemum and magnolia using the CGTase enzyme is a safe ingredient that does not cause toxicity to skin cells.

<Test Example 3> Melanin synthesis inhibitory effect test

The melanin synthesis inhibitory effect was tested by using the composite extract prepared in <Example 1> and <Comparative Example 1> as a sample.

B16F10 cells were divided into 3x10 ⁵ cells in a 60 mm culture dish, and then treated at a concentration of 200 μg/ml in the medium of Samples 1 to 5 of <Example 1> and <Comparative Example 1>. For a positive control, α-MSH (Sigma-Aldrich), a hormone that promotes melanin synthesis, was treated in the same cell at a concentration of 200 nM.

After the sample and α-MSH were treated in B16F10 melanoma cells, the cells were cultured for 48 hours at room temperature, the cells were harvested, washed twice with PBS, and the inhibition of melanin synthesis was measured for each sample. The results are shown in Table 3 below. indicated.

Sample name Melanin synthesis inhibition rate (%) Example 1 Enzyme Complex Extract 63.4 Comparative Example 1 simple complex extract 41.0

As can be seen from [Table 3], in the inhibition rate of melanin synthesis, it can be seen that the ability corresponding to <Example 1>, the chrysanthemum and magnolia enzyme complex extract is much superior to that of the <Comparative Example 1> simple complex extract. .

<Test Example 4> Antioxidant effect test

<Example 1> and <Comparative Example 1> were used as a sample to measure the antioxidant activity by DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging activity.

For the DPPH radical scavenging ability, the method of Mensor et al. was referred. After 20 μl of 80 μl of 0.2 mM DPPH ethanol solution was added to the fermentation broth samples of Preparation Examples 1 to 6, stirred for 1 minute, reacted in an incubator at 25° C. for 10 minutes, and absorbance was measured at 517 nm using an ELISA reader.

DPPH radical scavenging activity was expressed as a percentage according to [Formula 1] below, and the results are shown in [Table 4] below.

시료명 항산화효과(DPPH소거 활성율,%) 실시예1 효소 복합 추출물 87.3 비교예1 단순 복합 추출물 64.5 DPPH 라디칼 소거활성의 수치가 높다는 것은 항산화 효과가 높다는 것을 의미하며, 이는 멜라닌 생합성 과정의 산화반응의 억제를 통한 멜라닌 생성을 억제하여 미백효과를 나타내는 것이다.
DPPH 라디칼 소거활성의 수치가 높다는 것은 항산화 효과가 높다는 것을 의미하며, 이는 멜라닌 생합성 과정의 산화반응의 억제를 통한 멜라닌 생성을 억제하여 미백효과를 나타내는 것이다.
<시험예 5> Tyrosinnase 저해 효과 시험
<실시예 1>와 <비교예 1>을 통하여 제조한 각각의 추출물을 시료로 하여 Tyrosinase 저해 활성(Tyrosinase inhibitation activity)을 측정하였다.
Tyrosinase 저해 활성은 농도 별 시료 100㎕에 0.2M potassium phosphate buffer(pH6.5) 50㎕, 2mM L-tyrosine 50㎕, mushroom tyrosinase(110U/ml) 10㎕를 혼합하여 37℃에서 20분간 반응시킨 후 490nm에서 Multiplate Reader를 사용하여 흡광도를 측정하였다. Tyrosinase 저해활성(Tyrosinase inhibition activity)은 아래 [계산식 2]와 같이, 시료 무첨가구에 대한 시료 첨가구의 흡광도비로 계산하였으며 그 결과 [도 1]에 나타내었다.
[계산식 2]
Tyrosinnase 저해활성 = {1-(A-B)/(C-D)}×100
상기 식에서 A: 시료 첨가구의 흡광도, B: 공시료의 흡광도, C: 대조군 흡광도, D: 대조군 공시료 흡광도이다.
첨부한 [도 1]에 나타난 바와 같이 <실시예 1>과 <비교예 1>의 경우 타이로시나제 억제제의 함량이 많아 우수한 미백 효과를 나타낸다는 것을 확인할 수 있었으며, 특히 <실시예 1>의 경우 매우 많은 타이로시나제 억제제의 함량에 의해 매우 우수한 미백효과를 보인다는 것을 확인할 수 있다.
<실시예 2>
상기 <실시예 1>에서 제조된 능이, 국화 및 목련의 복합추출물이 포접된 포접물을 유효성분으로 하여 아래 [표 5]에 나타낸 조성성분 및 비율에 따라 수중유형 유화(O/W emulsion) 타입의 크림 제형의 화장품을 제조하였다.
조성성분 실시예 2 조성비(중량%) 세토스테아릴알코올 3.0 글리세릴스테이레이트 2.0 세테아릴올리베이트, 솔비탄올리베이트 1.0 폴리글리세릴-3 메틸글루코스디스테아레이트 1.5 스큐알렌 5.0 에틸헥실올리베이트, 소듐아크릴레이트코폴리머, 폴리글리세릴-4올리에이트 0.5 아크릴레이트/C10-30알킬아크릴레이트크로스폴리머 0.3 카프릴릭/카프릭트리글리세라이드 5.0 세틸에칠헥사노에이트 5.0 디메치콘 0.5 부틸렌글리콜 5.0 글리세린 8.0 1,2헥산디올 2.0 암모니움아크롤로일디메틸타우레이트/브이피코폴리머 0.3 알지닌 0.3 소듐아크릴레이트,폴리솔베이트80 0.3 효소 복합 추출물(실시예 1) 5.0 정제수 To 100 <실시예 3>
상기 <실시예 1>에서 제조된 능이, 국화 및 목련의 복합추출물이 포접된 포접물을 유효성분으로 하여 아래 [표 6]에 나타낸 조성성분 및 비율에 따라 샴푸 제형의 화장품을 제조하였다.
조성성분 실시예 3 조성비(중량%) 세토스테아릴알코올 3.0 소듐글루코네이트 0.50 소듐라우로일글루타메이트 0.10 클로페네신 0.20 소듐코코일이세티오네이트 3.30 폴리쿼터늄-10 0.20 다이소듐라우레스설포석시네이트,소듐라우릴설포아세테이트, 소듐클로라이드, 정제수 23.00 바바수아미도프로필베타인, 글리세린, 정제수 5.00 라우릴글루코사이드, 정제수 5.00 코코-베타인, 정제수 7.00 하이드롤라이즈드실크 0.10 폴리쿼터늄-7 0.30 하이드롤라이즈드케라틴 0.01 시트릭애씨드 0.10 소듐클로라이드 0.70 향료 0.50 에틸헥실글리세린 0.05 효소 복합 추출물(실시예 1) 5.00 정제수 To 100 <비교예 2>
<비교예 1>을 통하여 제조한 단순 복합 추출물을 유효성분으로 하여 수중유형 유화(O/W emulsion) 타입의 크림 제형의 화장품을 제조하였으며, 조성성분 및 비율은 <실시예 2>와 동일하게 하였다.
<비교예 3>
<비교예 1>을 통하여 제조한 단순 복합 추출물을 유효성분으로 하여 샴푸 제형의 화장품을 제조하였으며, 조성성분 및 비율은 <실시예 3>과 동일하게 하였다.
<시험예 6>
상기 <실시예 2>, <실시예 3>, <비교예 2> 및 <비교예 3> 의 수중유형 유화(O/W emulsion) 타입의 크림과 샴푸 제형의 화장품에 피부자극성 정도를 비교하기 위하여 인체에 대한 폐쇄첩포 시험(closed patch test)을 실시하였다.
건강한 성인 남녀 20명을 대상으로 하박부 안쪽에 각각의 시료를 0.2g씩 첩포하고 24시간 후 핀챔버를 제거한 후 4시간 경과한 다음 육안으로 피부상태를 확인하고, 그 결과를 아래 [표 7]에 나타내었다. 피부자극도는 하기 [계산식 3]에 의해 계산하였다.
[계산식 3]
피부자극도 = {(+-)수×1}+{(+)수×2}+{(++)수×3}/피험자수
시료명 피시험자수 판정결과 자극도 - + - + + + 실시예2 20명 20 0 - - 0.00 실시예3 20 0 - - 0.00 비교예2 20 0 - - 0.00 비교예3 20 0 - - 0.00 -:홍반이나 특이사항 없음, +-:주위보다 약간 붉어짐, +:주위보다 현저히 붉어짐
++:주위보다 심히게 붉어지고 부풀어 오름 상기 [표 7]에 나타낸 바와 같이, <실시예 2>, <실시예 3>, <비교예 2> 및 <비교예 3>의 화장품 조성물은 피부자극도가 매우 낮은 것으로 확인할 수가 있었다.
<시험예 7>
상기 <실시예 2>와 <비교예 2>에 대하여 실제 사용 시 피부 미백효과 및 보습효과를 알아보기 위하여 건강한 30 ~ 55세 성인여성 100명에 대하여 각각 <실시예 2>, <비교예 2>의 2그룹으로 나누어 인체 사용성 시험을 실시하였다.
상기 <실시예 2>와 <비교예 2>의 조성으로 제조된 시험품 50g씩을 설문지와 함께 나눠주고 아침과 저녁에 하루 2회씩 총 8주간 사용하게 한 후 설문지를 회수하여 분석하였다.
총 100명의 시험대상자 중 <실시예 2> 그룹의 50명, <비교예 2> 그룹의 50명의 설문지를 회수하여 분석한 결과를 아래 [표 8]에 나타내었다.
구분 응답자 수 매우 악화됨 약간 악화됨 변화없음 약간 좋아짐 매우 좋아짐 피부색 변화정도 실시예2 - - 8 12 30 비교예2 - - 19 21 10 피부 보습감 정도 실시예2 - - 10 23 17 비교예2 - - 8 28 14 상기 [표 8]에 나타낸 바와 같이, 본 발명에 따른 <실시예 2>의 크림 제형 화장료 조성물이 <비교예 2>의 크림에 비하여 피부색 변화, 피부 보습감개선 효능에 대한 사용자의 만족도가 높은 것을 확인할 수가 있다.[Formula 1]

Sample name Antioxidant effect (DPPH scavenging activity,%) Example 1 Enzyme Complex Extract 87.3 Comparative Example 1 simple complex extract 64.5 A high level of DPPH radical scavenging activity means that it has a high antioxidant effect, which shows a whitening effect by inhibiting melanin production through inhibition of the oxidation reaction of the melanin biosynthesis process.
A high level of DPPH radical scavenging activity means that it has a high antioxidant effect, which shows a whitening effect by inhibiting melanin production through inhibition of the oxidation reaction of the melanin biosynthesis process.
<Test Example 5> Tyrosinnase inhibitory effect test
Tyrosinase inhibition activity (Tyrosinase inhibition activity) was measured for each extract prepared in <Example 1> and <Comparative Example 1> as a sample.
Tyrosinase inhibitory activity was obtained by mixing 50 μl of 0.2M potassium phosphate buffer (pH 6.5), 50 μl of 2mM L-tyrosine, and 10 μl of mushroom tyrosinase (110U/ml) in 100 μl of each concentration sample and reacting at 37° C. for 20 minutes. Absorbance was measured at 490 nm using a Multiplate Reader. Tyrosinase inhibition activity was calculated as the absorbance ratio of the sample-added group to the non-sample-added group, as shown in Equation 2 below, and the results are shown in [FIG. 1].
[Formula 2]
Tyrosinnase inhibitory activity = {1-(AB)/(CD)}×100
In the above formula, A: the absorbance of the sample addition port, B: the absorbance of the blank sample, C: the control absorbance, D: the absorbance of the control blank sample.
As shown in the attached [FIG. 1], in the case of <Example 1> and <Comparative Example 1>, it was confirmed that the content of the tyrosinase inhibitor was high and thus an excellent whitening effect was exhibited, especially in <Example 1> In this case, it can be confirmed that a very good whitening effect is exhibited by the content of a very large amount of tyrosinase inhibitor.
<Example 2>
Oil-in-water emulsion (O/W emulsion) type according to the composition components and ratios shown in [Table 5] below, using the inclusion product containing the complex extract of Neungi, chrysanthemum, and magnolia prepared in <Example 1> as an active ingredient of cream formulations were prepared.
composition Example 2 Composition ratio (wt%) cetostearyl alcohol 3.0 glyceryl stearate 2.0 cetearyl olivate, sorbitan olivate 1.0 Polyglyceryl-3 methylglucose distearate 1.5 squalene 5.0 Ethylhexyl olivate, sodium acrylate copolymer, polyglyceryl-4 oleate 0.5 Acrylate/C10-30 alkyl acrylate crosspolymer 0.3 Caprylic/Capric Triglycerides 5.0 Cetylethylhexanoate 5.0 dimethicone 0.5 butylene glycol 5.0 glycerin 8.0 1,2 hexanediol 2.0 Ammonium acroloyl dimethyl taurate/vpicopolymer 0.3 Arginine 0.3 Sodium acrylate, polysorbate 80 0.3 Enzyme Complex Extract (Example 1) 5.0 Purified water To 100 <Example 3>
Using the inclusion product containing the complex extract of Neungyi, chrysanthemum, and magnolia prepared in <Example 1> as an active ingredient, cosmetics of a shampoo formulation were prepared according to the compositional components and ratios shown in [Table 6] below.
composition Example 3 Composition ratio (wt%) cetostearyl alcohol 3.0 sodium gluconate 0.50 Sodium Lauroyl Glutamate 0.10 Clofenesin 0.20 Sodium Cocoyl Cethionate 3.30 Polyquaternium-10 0.20 Disodium laureth sulfosuccinate, sodium lauryl sulfoacetate, sodium chloride, purified water 23.00 Barbasuamidopropyl betaine, glycerin, purified water 5.00 Lauryl Glucoside, Purified Water 5.00 Coco-betaine, purified water 7.00 Hydrolyzed Silk 0.10 Polyquaternium-7 0.30 Hydrolyzed Keratin 0.01 Citric Acid 0.10 sodium chloride 0.70 Spices 0.50 Ethylhexylglycerin 0.05 Enzyme Complex Extract (Example 1) 5.00 Purified water To 100 <Comparative Example 2>
A cosmetic of an oil-in-water emulsion (O/W emulsion) type cream formulation was prepared using the simple complex extract prepared in <Comparative Example 1> as an active ingredient, and the composition and ratio were the same as in <Example 2>. .
<Comparative Example 3>
Cosmetics in a shampoo formulation were prepared using the simple complex extract prepared in <Comparative Example 1> as an active ingredient, and the composition and ratio were the same as in <Example 3>.
<Test Example 6>
In order to compare the degree of skin irritation to the cosmetics of the oil-in-water emulsion (O/W emulsion) type cream and shampoo formulation of <Example 2>, <Example 3>, <Comparative Example 2> and <Comparative Example 3> A closed patch test was performed on the human body.
For 20 healthy adult males and females, 0.2 g of each sample was applied to the inside of the lower arm, 24 hours later, the pin chamber was removed, and 4 hours later, the skin condition was visually checked. shown in The degree of skin irritation was calculated by the following [Equation 3].
[Formula 3]
Skin irritation = {(+-) number×1}+{(+) number×2} + {(++) number×3}/number of subjects
Sample name number of subjects Judgment result stimulus degree - + - + + + Example 2 20 people 20 0 - - 0.00 Example 3 20 0 - - 0.00 Comparative Example 2 20 0 - - 0.00 Comparative Example 3 20 0 - - 0.00 -: No erythema or unusual, +-: Slightly reddened from the surrounding area, +: Remarkably reddened from the surrounding area.
++: Reddens and swells more than the surrounding area As shown in [Table 7], the cosmetic compositions of <Example 2>, <Example 3>, <Comparative Example 2> and <Comparative Example 3> were confirmed to have very low skin irritation.
<Test Example 7>
<Example 2> and <Comparative Example 2> for 100 healthy 30-55 year old adult women, respectively, in order to examine the skin whitening effect and moisturizing effect during actual use for <Example 2> and <Comparative Example 2> was divided into 2 groups and tested for human usability.
50 g each of the test article prepared in the composition of <Example 2> and <Comparative Example 2> was distributed with the questionnaire, and the questionnaire was collected and analyzed after allowing it to be used twice a day in the morning and evening for a total of 8 weeks.
[Table 8] shows the results of collecting and analyzing questionnaires from 50 people in the <Example 2> group and 50 people in the <Comparative Example 2> group out of a total of 100 test subjects.
division number of respondents very bad slightly worse no change slightly better very good degree of skin color change Example 2 - - 8 12 30 Comparative Example 2 - - 19 21 10 level of skin moisture Example 2 - - 10 23 17 Comparative Example 2 - - 8 28 14 As shown in [Table 8], it is confirmed that the cream-formed cosmetic composition of <Example 2> according to the present invention has higher user satisfaction with respect to the skin color change and skin moisturizing effect than the cream of <Comparative Example 2>. can be

Claims (7)

a) 1 to 10 parts by weight of Neungyi, 1 to 10 parts by weight of chrysanthemum, and 1 to 10 parts by weight of magnolia by warming and extracting with purified water or oily hot spring water to obtain a complex extract of Neungi, Chrysanthemum and Magnolia;
b) 1.0 to 5.0 parts by weight of dextrin containing cyclodextrin glucanotransferase at a concentration of 0.01 to 1.0 W/V% with respect to 100 parts by weight of the complex extract of the first step, temperature 30 to 80° C., pH 3.0 to 8.0 A second process of producing a cyclodextrin containing the complex extract of Neungyi, Chrysanthemum and Magnolia by inclusion of the complex extract of the first process by reacting for 5 to 24 hours under the reaction conditions of
c). For antioxidant, skin whitening, or moisturizing, characterized in that the second step includes a third step of preparing a cosmetic composition containing cyclodextrin containing a complex extract of chrysanthemum and magnolia as an active ingredient Method for producing a cosmetic composition, characterized in that applied to. The method according to claim 1, wherein the dextrin is applied to antioxidation, skin whitening, or moisturizing, characterized in that DE 1 to DE 60 dextrin is selected. delete Contains 100 parts by weight of a complex extract of Neungyi, chrysanthemum and magnolia extracted from 1 to 10 parts by weight of chrysanthemum, 1 to 10 parts by weight of chrysanthemum, and 1 to 10 parts by weight of magnolia at a concentration of 0.01 to 1.0 W/V% of cyclodextrin glucanotransferase. A dextrin containing 1.0 to 5.0 parts by weight of dextrin and cyclodextrin containing complex extracts of Neungyi, Chrysanthemum and Magnolia as an active ingredient, characterized in that the active ingredient contains 0.01 to 20% by weight based on the total weight of the cosmetic. A cosmetic composition, characterized in that it is applied for antioxidant, skin whitening or moisturizing. delete delete The method according to claim 4, wherein the cosmetic composition is a lotion, emulsion, cream, essence, pack, mask, foundation, sunscreen, BB cream, shampoo, treatment, body wash, body lotion, hand lotion and any one selected from skin cleanser formulations Cosmetic composition, characterized in that the formulation of.

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