KR102277369B1 - Composition for preventing or improving wrinkle comprising Tradescantia pallida extract - Google Patents

Abstract

The present invention relates to a composition for preventing or improving wrinkle comprising the Palida frequent chicken gabi extract as an active ingredient, and according to the present invention, the Palida purple chicken gabi extract exhibits antioxidant activity, collagenase activity inhibitory ability, and elastase activity inhibitory activity. It can be usefully used as a cosmetic composition for preventing or improving skin wrinkles, a pharmaceutical composition for preventing or treating skin wrinkles, a health food composition for preventing or improving skin wrinkles, and the like.

Description

Composition for preventing or improving wrinkle comprising an extract of palidajajudakgabi as an active ingredient {Composition for preventing or improving wrinkle comprising Tradescantia pallida extract}

The present invention relates to a composition for preventing or improving wrinkles comprising an extract of palidajajudakgabi extract as an active ingredient.

The skin is one of the main tissues that perform the physical and biochemical defense function of the human body, and is composed of the epidermis, dermis and hypodermis. An extracellular matrix (ECM) composed of collagen) and elastin exists to control the elasticity of the skin and is involved in the creation of wrinkles. Collagen, which occupies more than 90% of the dermis, performs functions such as the mechanical firmness of the skin, the resistance of connective tissue and the binding force of tissues, support of cell adhesion, and induction of cell division and differentiation. Elastin is an insoluble fibrous protein that encapsulates collagen bundles.

On the other hand, in modern society, erythema reaction, pigmentation reaction, skin aging, skin cancer, etc. are caused by the increase of the average lifespan, the increase in UV exposure opportunities due to the increase in leisure activities, the destruction of the ozone layer due to environmental pollution, and the increase in the absolute amount of UV. Change is increasing. In addition, as the age increases, the amount of fibroblasts and the elasticity of the skin decrease due to the action of fibroblasts and the decrease in the number of cells, and the structure of the stratum corneum occurs due to the loss of moisture in the skin cells. As a result, lipids, proteins, polysaccharides and nucleic acids, which are the main constituents of the skin, are damaged, and skin cells and tissues are destroyed and skin aging occurs.

It is known that skin aging is caused by loss of biobound water in skin cells, structural change in the stratum corneum of the skin, reduction in differentiation of epidermal cells, and degradation of biosynthetic functions of proteins and intercellular substances by fibroblasts in the dermis. In order to delay and inhibit skin aging, it is necessary to suppress excess reactive oxygen species (ROS) in the skin as well as in vivo or to remove free radicals generated, and chain cleavage of collagen and elastin It should be able to suppress the deformation of the cross-links.

Accordingly, as the number of women who want young and elastic skin increases, the market scale of functional food and cosmetics is expanding, and research using natural extracts is in progress rapidly. Natural extracts contain various physiologically active ingredients, and these ingredients have antioxidant, antibacterial, and anti-inflammatory effects, and thus are being used in various ways as cosmetics and skin care materials.

On the other hand, Tradescantia pallida is a fleshy evergreen succulent plant belonging to the Rosaceae family, native to northeastern Mexico. The flower has three pink petals and yellow stamens, and the leaf stem is purple. It can be easily propagated through cuttings and is cultivated for ornamental purposes. The study on the physiological activity of Palidajajabijabi was recently reported on the antioxidant and antibacterial activity of leaves collected in Malaysia, but since its introduction in Korea, there has been no related research until now.

Republic of Korea Patent Registration No. 10-1152326 (Registered on May 25, 2012)

It is an object of the present invention to provide a cosmetic composition for preventing or improving skin wrinkles, a pharmaceutical composition for preventing or treating skin wrinkles, or a health food composition for preventing or improving skin wrinkles.

Another object of the present invention is to provide a reagent composition for inhibiting collagenase activity, or a reagent composition for inhibiting elastase activity.

In order to achieve the above object, the present invention provides a cosmetic composition for preventing or improving skin wrinkles comprising an extract of Tradescantia pallida as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for preventing or treating skin wrinkles comprising an extract of Tradescantia pallida as an active ingredient.

In addition, the present invention provides a health food composition for preventing or improving skin wrinkles comprising an extract of Tradescantia pallida as an active ingredient.

In addition, the present invention provides a reagent composition for inhibiting collagenase activity comprising an extract of Tradescantia pallida as an active ingredient.

In addition, the present invention provides a reagent composition for inhibiting elastase activity comprising an extract of Tradescantia pallida as an active ingredient.

According to the present invention, the extract of palidaja gaebi extract exhibits antioxidant activity, collagenase activity inhibitory activity, and elastase activity inhibitory activity and thus has an excellent skin wrinkle improvement effect, a cosmetic composition for preventing or improving skin wrinkle, for preventing or treating skin wrinkle It can be usefully used as a pharmaceutical composition, a health food composition for preventing or improving skin wrinkles, and the like.

1 is a view confirming the total polyphenol content of the palidajaju chicken gabi extract.
Figure 2 confirms the total flavonoid content of the palidajaju chicken gabi extract.
Figure 3 is a confirmation of the electron donating ability of the palidajaju chicken gabi extract.
Figure 4 confirms the collagenase activity inhibitory ability of the palidazaju chicken gae extract.
Figure 5 confirms the elastase activity inhibitory ability of the palidajaju chicken gae extract.

The inventors of the present invention prepared an extract of falida jasmine chicken using ethanol, methanol or ethyl acetate, and confirmed the skin wrinkle improvement effect from the antioxidant ability, collagenase activity inhibition ability, and elastase activity inhibition ability of the extract. of was completed.

Accordingly, the present invention provides a cosmetic composition for preventing or improving skin wrinkles comprising an extract of Tradescantia pallida as an active ingredient.

The extract may be extracted with any one or more solvents selected from the group consisting of ethanol, methanol and ethyl acetate, but is not limited thereto.

The extract may be included in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total composition, but is not limited thereto.

The extract may prevent or improve skin wrinkles by inhibiting the activities of collagenase and elastase.

When the composition of the present invention is a cosmetic composition, the cosmetic composition may include conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers in addition to the active ingredients. In addition, the cosmetic composition may further include a skin absorption accelerator to enhance the effect.

The formulation of the cosmetic composition may be prepared in any formulation conventionally prepared in the art, for example, the cosmetic composition is a lotion, emulsion, skin, toner, lotion, essence, sunscreen, cream, makeup base, foundation , powder, pack, gel, ointment, patch, stick, shampoo, rinse, spray, makeup remover and cleaning agent, etc., but is not limited thereto.

When the formulation is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. .

When the formulation is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane/butane or a propellant such as dimethyl ether.

When the formulation is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3 -Butylglycol oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose , aluminum metahydroxide, bentonite, agar or tracanth may be used.

In addition, the present invention provides a pharmaceutical composition for preventing or treating skin wrinkles comprising an extract of Tradescantia pallida as an active ingredient.

When the composition of the present invention is a pharmaceutical composition, for administration, a pharmaceutically acceptable carrier, excipient or diluent may be included in addition to the active ingredients described above. The carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The pharmaceutical composition of the present invention can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, or sterile injection solutions, respectively, according to conventional methods. . In detail, when formulating, it can be prepared using a diluent or excipient such as a filler, a weight agent, a binder, a wetting agent, a disintegrant, a surfactant, etc. commonly used. Solid preparations for oral administration include, but are not limited to, tablets, pills, powders, granules, capsules, and the like. Such a solid preparation may be prepared by mixing at least one or more excipients, for example, starch, calcium carbonate, sucrose, lactose, gelatin, etc. in addition to the active ingredient. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. It may be prepared by adding various excipients, for example, wetting agents, sweetening agents, fragrances, preservatives, etc., in addition to liquids for oral use and liquid paraffin. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations, and tasks. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used. As the base of the suppository, Witepsol, Macrosol, Tween 61, cacao butter, laurin fat, glycerogelatin, etc. can be used.

A suitable dosage of the pharmaceutical composition of the present invention varies depending on the patient's condition and weight, the degree of disease, drug form, and time, but may be appropriately selected by those skilled in the art, and the daily dosage of the composition is preferably It is 0.001 mg/kg to 50 mg/kg, and may be administered once a day or divided into several times a day as needed.

In addition, the present invention provides a health food composition for preventing or improving skin wrinkles comprising an extract of Tradescantia pallida as an active ingredient.

When the composition of the present invention is a health food composition, various nutrients, vitamins, minerals (electrolytes), synthetic flavoring agents and flavoring agents such as natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.), pectic acid and salts thereof , alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, it may contain the pulp for the production of natural fruit juices, synthetic fruit juices and vegetable beverages. These components may be used independently or in combination. In addition, the health food composition may be in the form of any one of meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, gum, ice cream, soup, beverage, tea, functional water, drink, alcohol and vitamin complex. can

In addition, the health food composition may additionally contain food additives, and the suitability as a food additive is determined according to the general rules and general test methods of the Food Additives Code approved by the Ministry of Food and Drug Safety, unless otherwise specified. It is judged according to the standards and standards.

Items listed in the Food Additives Codex, for example, chemical synthetic products such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid, depigmentation, licorice extract, crystalline cellulose, high cooling pigment, natural additives such as guar gum, L-glutamic acid Mixed preparations, such as a sodium preparation, an alkali agent for noodles, a preservative preparation, and a tar dye preparation, etc. are mentioned.

In this case, the composition according to the present invention to be added to food in the process of preparing the health food composition may appropriately increase or decrease its content as necessary.

In addition, the present invention provides a reagent composition for inhibiting collagenase activity comprising an extract of Tradescantia pallida as an active ingredient.

In addition, the present invention provides a reagent composition for inhibiting elastase activity comprising an extract of Tradescantia pallida as an active ingredient.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example 1: Reagents and Instruments

Phenol (Folin &Ciocalteu's phenol reagent), dimethyl sulfoxide (DMSO), diethylene glycol, potassium persulfate (potassium potroxodisulfate), 1,1-diphenyl-2-picrylhydrazyl (1,1-diphenyl-2-picrylhydrazyl; DPPH), tannic acid, rutin, 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg(4-phenylazobenzyloxycarbonyl- Pro-Leu-Gly-Pro- D-Arg), N - succinyl _{- (L-Ala) 3 -} p - ( nitro not fluoride) [N -succinyl- (L-Ala ) 3 - p - (nitroanilide)] , collagenase (from Clostridium histolyticum ), elastase (from porcine pancreas) products of Sigma (St. Louis, MO, USA) were used, and for other general reagents, premium products were used.

Example 2: Preparation of Palidajaju Chicken Gabi Extract

In order to prepare the T. pallida extract, the above-ground part of the pallida common chicken gae growing in the glass greenhouse of the College of Life and Resources Science, Jeju University was collected and dried in a dryer. The dried Palidajaja Chicken Gaebi was pulverized with a blender, sieved through a 500 μm sieve, and extracted twice with 100 mL of ethanol, methanol or ethyl acetate per 10 g for 24 hours each, followed by concentration with a vacuum concentrator. Samples that were not completely concentrated were dried with a freeze dryer, and each of T. pallida ethanol extract (TPEE), T. pallida methanol extract (TPME), and ethyl acetate A sample of the extract ( T. pallida ethyl acetate extract; TPAE) was prepared. After that, the sample was dissolved in DMSO, diluted with distilled water from 1000 μg/mL to 62.5 μg/mL, and then used for the experiment.

Example 3: Antioxidant activity analysis

1) Analysis of total polyphenol content

The total polyphenol content was measured by the Folin-Denis method (Folin & Denis, 1912). 1 mL of folin-reagent was added to 1 mL of the sample diluted to 200 μg/mL, reacted for 3 minutes at room temperature, 1 mL of 10% Na ₂ CO ₃ was added, and left at room temperature for 1 hour. Then, absorbance was measured at 725 nm. The calibration curve was prepared using tannic acid.

As a result, as shown in FIG. 1 , it was confirmed that the total polyphenol content was 298.7 tannic acid equivalent (TAE) mg/g for TPEE, 418.4 TAE mg/g for TPME, and 430.1 TAE mg/g for TPAE.

2) Total flavonoid content

Total flavonoid content was determined by the method of Davies et al. (1980). 1 mL of diethylene glycol was added to 100 μL of a sample diluted to 200 μg/mL, and after reaction at room temperature for 5 minutes, 100 μL of 1 N NaOH solution was added and left at 37° C. for 1 hour. Then, absorbance was measured at 420 nm. A calibration curve was created using a routine.

As a result, as shown in FIG. 2 , it was confirmed that the total flavonoid content was 180.5 rutin equivalent (RE) mg/g for TPEE, 247.1 RE mg/g for TPME, and 359.1 RE mg/g for TPAE.

3) Electron donating ability (DPPH radical scavenging ability)

The electron donating ability was measured by the method of Blois (1958). To a 96-well plate, add 50 µL of 0.2 mM DPPH solution in ethanol and 100 µL each of Pallidaceae extract at a concentration of 62.5, 125, 250, 500, and 1000 µg/mL, and leave it for 30 minutes in a light-shielded state 517 Absorbance was measured in nm. GraphPrism software Ver. 7 (Graphpad Software, lnc., La Jolla, CA, USA) was used _{to calculate SC 50} (50% scavenging concentration, 50% scavenging concentration of free radicals).

As a result, as shown in FIG. 3 , the palidajajajagabi extract showed a positive (+) dose-response correlation in a concentration-dependent manner, and ascorbic acid (AA) as a positive control and TPEE, TPME, and TPAE extracts were It was confirmed that the electron donating ability was 97.5, 70.4, 66.7, and 91.9% at 1000 μg/mL, respectively. After calculating the _{SC 50, TPEE, TPME, SC} 50 for TPAE extract was 664.8, 660.8, was 277.3 μg / mL. SC ₅₀ of AA was not calculated, but was found to be less than 125 μg/mL.

Example 4: Enzyme activity assay

1) Inhibitory ability of collagenase activity

4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly- in 0.1 M Tris-HCl buffer (pH 7.5) containing 100 µL of sample diluted to 62.5, 125, 250, 500, and 1000 µg/mL and 4 mM CaCl ₂ After mixing 250 µL of a substrate solution in which Pro-D-Arg (0.3 mg/mL) was dissolved, 150 µL of collagenase (0.2 mg/mL) was added, followed by reaction at room temperature for 20 minutes. Then, 1 mL of 6% citric acid was added, and 1.5 mL of ethyl acetate was added to stop the reaction, and then the supernatant was taken and absorbance was measured at 320 nm. Ascorbic acid was used as a positive control. _{IC 50} (50% inhibitory concentration, 50% inhibitory concentration of collagenase activity) was calculated using GraphPrism software.

As a result, as shown in FIG. 4 , the palidajajagabi extract showed a positive (+) dose-response correlation in a concentration-dependent manner, and ascorbic acid (AA) as a positive control and collagenase for TPEE, TPME, and TPAE extracts It was confirmed that the activity inhibiting ability was 65.9, 63.7, 76.0, and 36.4% at 1000 μg/mL, respectively. _{IC 50} of the AA, TPEE, and TPME extracts were calculated to be 248.8, 503.9, and 217.7 μg/mL, respectively, and the IC ₅₀ of the TPAE extract was not calculated, but was found to be higher than 1000 μg/mL. That is, it was found that the TPME extract had better ability to inhibit collagenase activity than the positive control AA.

2) Elastase activity inhibition ability

15 µL of sample diluted to 62.5, 125, 250, 500, and 1000 µg/mL and 5 µL of 0.2 unit of porcine pancreas elastase dissolved in 100 mM Tris-HCl buffer (pH 8) and N- After mixing 20 μL of a substrate solution in which succcinyl-(L-Ala) ₃ - p- nitroa-nilide (0.5 mg/mL) was dissolved, the mixture was reacted at 37°C for 10 minutes. After cooling on ice for 5 minutes, absorbance was measured at 410 nm. Ascorbic acid was used as a positive control. _{IC 50} (50% inhibitory concentration, the concentration inhibiting elastase activity by 50%) was calculated using GraphPrism software.

As a result, as shown in FIG. 5 , the palidajajajagabi extract showed a positive (+) dose-response correlation in a concentration-dependent manner, and ascorbic acid (AA) as a positive control and elastase for TPEE, TPME, and TPAE extracts It was confirmed that the activity inhibition ability was 40.8, 79.8, 83.3, and 69.1% at 1000 μg/mL, respectively. _{IC 50} of TPEE, TPME, and TPAE extracts were calculated as 537.3, 388.1, and 231.9 μg/mL, respectively, and IC ₅₀ of AA was not calculated, but was found to be greater than 1000 μg/mL. That is, the TPEE, TPME, and TPAE extracts were found to have superior ability to inhibit elastase activity than the positive control AA.

Therefore, from the above results, it was confirmed that the palidajajajajabi chicken extract can effectively inhibit the generation of skin wrinkles.

As the specific parts of the present invention have been described in detail above, for those of ordinary skill in the art, these specific descriptions are only preferred embodiments, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present invention.

Claims (8)

As a cosmetic composition for preventing or improving skin wrinkles comprising an extract of Tradescantia pallida as an active ingredient,
The extract is a cosmetic composition for preventing or improving skin wrinkles, characterized in that it inhibits the activity of collagenase and elastase. The cosmetic composition for preventing or improving skin wrinkles according to claim 1, wherein the extract is extracted with any one or more solvents selected from the group consisting of ethanol, methanol and ethyl acetate. The cosmetic composition for preventing or improving skin wrinkles according to claim 1, wherein the extract is included in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total composition. delete delete As a health food composition for preventing or improving skin wrinkles comprising an extract of Tradescantia pallida as an active ingredient,
The extract is a health food composition for preventing or improving skin wrinkles, characterized in that it inhibits the activity of collagenase and elastase. delete delete

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