KR102569246B1 - Composition for skin whitening and wrinkle improvement containing saussurea neoserrata extract - Google Patents

Abstract

The present invention relates to a composition for skin whitening and wrinkle improvement, in the case of containing sangolchi extract as an active ingredient, there is no concern about side effects due to long-term use due to the use of natural products, and skin whitening improvement activity through tyrosinase inhibitory activity In addition to exhibiting anti-wrinkle activity through elastase inhibitory activity, it can also exhibit anti-obesity activity through the effect of inhibiting fat formation.

Description

Composition for skin whitening and wrinkle improvement containing an extract of sangolchwi

The present invention relates to a composition for skin whitening and wrinkle improvement. More specifically, it relates to a composition capable of exhibiting skin whitening and anti-wrinkle activity and also exhibiting anti-obesity activity, by including an extract of Sangolchi as an active ingredient.

Various factors are involved in determining the skin color of a person. Among them, the activity of melanocytes that produce melanin pigment, the distribution of blood vessels, the thickness of skin, and pigment-related factors inside and outside the human body, such as carotenoids and bilirubin, are important. In particular, the most important factor is a pigment called melanin, which is produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. It is known that pigmentation of the skin is caused by stimuli such as ultraviolet rays or inflammation, and also by factors such as genetic factors, hormones, foods, and chemicals. In particular, inflammation-related factors such as prostaglandins, histamine, alpha-MSH, and NO (Nitric oxide) are involved in abnormal skin pigmentation symptoms such as melasma, freckles, and pigmentation, resulting in melanocyte formation. ) is well known to activate melanin production in In addition, there is also a report that hyperpigmentation is accompanied by immunological mediators even in the process of tissue damage and regeneration.

Looking at melanin pigment synthesis in detail, when melanocytes are stimulated by various factors, tyrosinase produces melanin in melanosomes. That is, tyrosine in melanocytes is converted into dopa by the action of tyrosinase, then converted into dopaquinone by the action of dopaoxidase, and then tyrosinase-related protein-1 (tyrosinase-related protein-1). Melanin is produced by the action of protein-1 (hereinafter referred to as TRP-1) and tyrosinase-related protein-2 (hereinafter referred to as TRP-2). In this series of reactions, since tyrosinase activates the rate-limiting reaction of the melanin pigment formation process, melanin formation is primarily controlled by the activity and expression of tyrosinase. In addition, TRP2, known as DOPA chromium transmutase, activates the reaction of DOPA chromium to 5,6-dihydroxyindole-2-carboxylic acid (DHICA), and TRP-1 oxidizes DHICA to carboxyl indole-quinone. let it As a result, TRP-1 and TRP-2 are involved in the polymerization reaction, which is the final stage of formation of melanin, an insoluble pigment. It has been reported that these tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2) increase their activity by inflammatory mediators, thereby increasing the synthesis of melanin.

Currently developed whitening agents include kojic acid, arbutin, glutathione, vitamin C, etc., but they do not have a whitening effect satisfactory to consumers, and their usage is limited due to skin safety. Therefore, various attempts have been made for each stage of melanin synthesis.

In addition, as the elderly population increases, skin beauty and anti-aging, which can be a measure of aging, are emerging as major concerns. Wrinkles of the skin and reduced antioxidant activity in the body due to aging can be used as a measure of health. Skin aging can be divided into intrinsic aging and extrinsic aging according to factors. Intrinsic aging is known to be caused by changes in physiological functions of the skin epidermis and dermis according to age, and extrinsic aging is known to be caused by changes in physiological functions of the skin caused by environments such as air pollution, exposure to ultraviolet rays, and stress. The common mechanism of this aging is continuous exposure to oxidative stress, increasing free radicals in the body, and increasing the activity of elastase that decomposes elastin, a connective tissue of the dermis. , Degradation of hyaluronic acid can be explained by increased activity of hyaluronidase. In other words, skin aging can be explained as the formation of wrinkles by destroying elastin and hyaluronic acid, which are the basic structures of the skin, to break down the dermis and dry the skin. In addition, radicals in the body are involved in the melanin formation process and activate tyrosinase to cause spots and freckles.

In our daily lives, we are always exposed to harmful effects caused by active oxygen, and the harmful effects caused by aging gradually accumulate and the antioxidant capacity gradually decreases. Under normal body conditions, the antioxidant protection system removes active oxygen through enzymatic (Superoxide dismutase, etc.) and non-enzymatic (Polyphenols, Flavonoids, etc.) reactions. Therefore, in order to exhibit antioxidant activity, antioxidant enzyme reactions such as SOD must be activated or non-enzymatic substances that act directly as antioxidants must be consumed. In this regard, research on mechanisms for improving skin wrinkles and the like has been actively conducted, and research on cosmetics and health functional foods showing biochemical efficacy and effects is being conducted.

On the other hand, obesity refers to a case in which fat is excessively accumulated in the body, and according to national health statistics, the prevalence of obesity is increasing from 26.0% in 1998 to 31.4% in 2011. In particular, obesity has been found to be related to complications of various adult diseases. For example, it has been reported that obese patients have a mortality rate about 2 times higher in the case of liver cirrhosis, 1.6 times in the case of cerebrovascular disease, and 1.8 times higher in the case of coronary artery disease than those of normal weight.

Currently, diet therapy, exercise therapy, and drug therapy are being tried as a treatment method for obesity, and among these, obesity treatment used as drug therapy can control appetite, hinder digestion and absorption of fat, increase energy consumption, or decrease lipid metabolism. Although it was developed targeting control, etc., various side effects exist. Xenical (Roche) inhibits fat absorption by inducing inhibition of lipase, which is separated from the pancreas and digestive system, but shows a low weight loss effect of only 2-3%, accompanied by side effects such as frequent diarrhea and steatorrhea. In addition, Reductil (Abbott) suppresses appetite by suppressing the reuptake of serotonin and noradrenaline, so it has a weight loss effect of 5 to 10%, but has side effects of cardiovascular diseases such as stroke and myocardial infarction. It was withdrawn from the market by the EMA and the US Food and Drug Administration (FDA), recommending suspension of prescription and use, and voluntary recall. In addition, many of the products developed as anti-obesity drugs have been banned from sale due to serious side effects. For example, aminophylline has been reported to have a wide range of side effects across the mental nervous system, circulatory system, and digestive system despite its excellent body fat decomposition effect, and fenfluramine, dexfenfluramine, topiramay, or epidrine have also been judged to be unsuitable drugs for obesity treatment. sale is prohibited. As Western medicine has shown its limitations in overcoming side effects and chronic diseases of synthetic drugs, the value of natural food and drug products with anti-obesity effects is emerging.

Accordingly, in the present invention, the present invention was completed by including the extract of Sangolchi as a natural product to exhibit anti-obesity activity while exhibiting skin whitening activity and wrinkle improvement activity.

KR 10-1937331 B1 KR 10-1936294 B1

An object of the present invention relates to a composition for skin whitening and anti-wrinkle containing an extract of Sangolchi.

Another object of the present invention is to provide a cosmetic composition, a food composition, and a health functional food, which contain an extract of sangolchi as an active ingredient and have no side effects, no problems even when taken for a long time, and excellent skin whitening and wrinkle improvement effects.

In order to achieve the above object, the composition for skin whitening and anti-wrinkle according to an embodiment of the present invention is to include an extract of Sangolchwi as an active ingredient.

The composition exhibits skin whitening and anti-wrinkle activity by inhibiting tyrosinase and elastase.

The composition exhibits anti-obesity activity by inhibiting the formation of adipocytes in adipocytes.

The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohol, and mixtures thereof.

A cosmetic composition according to another embodiment of the present invention includes the composition for skin whitening and wrinkle improvement.

The cosmetic composition is a formulation selected from the group consisting of skin, serum, lotion, emulsion, cream, essence, lotion, foundation, pack, soap, surfactant-containing cleansing, spray and powder.

A food composition according to another embodiment of the present invention includes the composition for skin whitening and wrinkle improvement.

Hereinafter, the present invention will be described in more detail.

As used herein, the term 'extract' has a meaning commonly used in the art as a crude extract in the art as described above, but also includes fractions obtained by additional fractionation of the extract in a broad sense.

That is, the natural extract includes not only those obtained by using an extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc. The fraction obtained through the purification method is also included in the natural extract of the present invention.

The natural extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

In the present specification, the term 'comprising as an active ingredient' means containing an amount sufficient to achieve the efficacy or activity of the following natural extract.

A composition for skin whitening and wrinkle improvement according to an embodiment of the present invention includes an extract of Sangolchwi as an active ingredient.

The sangolchwi (saussurea neoserrata nakai) grows in deep mountains and has a height of 50 to 110 cm. The stem stands straight and sometimes branches from the upper part. The base of the leaf flows into the stem and becomes the wing of the stem. The leaves attached to the root and the lower part are removed when the flower blooms, and the leaves attached to the stem are alternate and lanceolate or oval-shaped lanceolate, 12 to 15 cm long. The tip is pointed and the bottom narrows. It gets smaller as it goes to the upper part, and there are curly hairs on the back side and sharp sawtooth on the edge. Also, the flowers bloom in purple in August or September. Head flowers hang at the ends of branches and main stems in a corymbose inflorescence, and the flower diameter is 8 to 10 mm. The gun is barrel-shaped, 8 to 9 mm long and 4 to 5 mm in diameter. The bracts are lined up in 4 lines, the edges are purple, and white hairs like spider webs grow. Fruits are achene, about 5mm long, with black brown lines. The crown hair is 2 lines and brown. Young shoots are edible. This species is endemic to Korea and is distributed in northern regions.

The present invention can exhibit excellent skin whitening and anti-wrinkle effects by including the sangolchwi extract.

The composition exhibits skin whitening and anti-wrinkle activity by inhibiting tyrosinase and elastase.

The tyrosinase (Tyrosinase) is an enzyme involved in the most important initial determination step in the melanin biosynthetic pathway in the human body. Aging of the human skin is a phenomenon that occurs naturally over time, but as the skin becomes thinner due to pollutants, sunlight, and ultraviolet rays, and spots, freckles, and age spots increase, the demand for skin whitening is increasing. In particular, the ultraviolet rays To protect against damage caused by exposure, melanin pigment is produced. The oxidative stress generated by ultraviolet rays increases the activity of melanosomes, which are small ducts of melanocytes in epidermal or dermal cells, and the activity of the melanosomes is tyrosinase ( tyrosinase) to produce more melanin pigments. Accordingly, whitening activity may be exhibited by inhibiting the activity of tyrosinase.

On the other hand, skin connective tissue related to skin wrinkle formation is mainly composed of fibrous components such as collagen fiber, reticular fiber, and elastic fiber, and collagen and elastin in dermal tissue is an important fibrous tissue that works closely with each other to maintain the flexibility and elasticity of the skin.

In particular, the elastin is a major fibrous tissue that influences the elasticity of the skin, and its amount is about 2% to form a network tissue supporting the epidermis, playing a very important role in skin elasticity. Elastin is decomposed by a degrading enzyme called elastase, and is produced by fibroblasts until the age of 18 to 19, but from then on, production stops and only the already produced elastin is responsible for skin elasticity. Resilience is difficult to recover. Causes of the weakening of the elastin include deficiency and aggregation of elastin fibers and significant increase in the activity of elastase, which is the main factor in skin wrinkle formation, thereby inhibiting elastase production to create skin wrinkles can suppress

In other words, in the case of the composition containing the extract of Sangolchi of the present invention, the whitening activity by inhibiting tyrosinase activity is excellent, and the skin wrinkle formation inhibitory activity through elastase production inhibitory activity can also be exhibited to a superior extent. do.

The composition exhibits anti-obesity activity by inhibiting the formation of adipocytes in adipocytes.

Adipose tissue including the adipose cells is divided into white adipose tissue (WAT) and brown adipose tissue (BAT), and most adipose tissue stores energy in the body and adiponectin, leptin, etc. While responsible for secreting the same hormone, BAT is responsible for thermogenesis. Due to its ability to store energy, when surplus calories are excessive, expansion of WAT occurs through hypertrophy, in which the size of adipocytes increases, and differentiation in which the number of adipocytes increases (hyeperplasia, adipogenesis). .

Adipogenesis, which represents differentiation in which the number of adipocytes increases, refers to a process in which preadipocytes differentiate into adipocytes, and 3T3-L1 cells are widely used in anti-obesity research as a preadipocyte model optimized for adipogenesis research. there is. Proliferatively stopped preadipocytes begin to differentiate by stimulation of adipogenic hormonal cocktails including 3-isobutyl-1-methyl-xanthine (IBMX), dexamethasone and insulin. The process of differentiation of preadipocytes goes through organically linked early, middle, and late stages to generate fat globules to become mature adipocytes with the ability to accumulate fat and endocrine function.

Accordingly, in the case of the composition containing the extract of Sangolia of the present invention, it can exhibit excellent anti-obesity activity by inhibiting the formation of fat globules in adipocytes as well as skin whitening and wrinkle-reducing activities.

The skin whitening and anti-wrinkle composition of the present invention may further include an extract of a mortar and pestle, an extract of Orchid alfalfa, and an extract of callephensis rue.

Jeolgutdae (Echinops setifer Iljin) is a perennial plant of the dicotyledonous plant Asteraceae, also called garichwi or jeolgudae. Grows in sunny meadows. It is about 1m high, has slightly branched branches, and is covered with cottony hairs, so the whole is white. The leaves from the root have long petioles and are green on the surface and white on the back. Also, the edge is cracked like a thistle and has thorns. Leaves from the stem have no petiole, are long oval, and split into 5-6 pairs. The flower blooms in July-August, is man-colored, and has a diameter of about 5cm, and is an ornamental flower. The end of the corolla is split into 5 pieces and rolled back, and the end of the gun barrel becomes like a thorn. The fruit is an achene with dense hairs, and the hairs are like scales. The young leaves are edible and the root is used for scraping. Distributed in Korea and Japan.

Cacalia auriculata var. alata Nakai is a perennial plant of the dicotyledonous plant Asteraceae that grows in deep mountains. The stem stands upright, the height is 1~2m, the diameter of the lower part is 1.5cm, and the branches diverge from the upper part. Leaves are alternate, triangular, pointed at the end, slightly heart-shaped at the bottom, and saw-toothed at the edge. The leaf attached to the middle part of the stem is 15 cm long and 25 cm wide, and the petiole has wings, and the wings at the bottom are wide and cover the stem. Flowers bloom in August in yellow color, and capitulum runs at the end of the stem forming a panicle. The gun pieces are 5 to 6 pieces, 13 to 15 cm long, and arranged in a single line. There are 1 or 2 guns under the gun, and the head painting is made up of about 10 ornamental flowers. The fruits are achene, line-shaped, with lines, hairless, and the hairs are white and 8mm long. eat young leaves As a Korean endemic species, it is distributed in Unseonryeong, Potaesan, and Choigaryeong, Hamgyeongnam-do.

Ruta chalepensis L. is a citrus flowering plant known as Ruta Fringed and is a perennial plant of the genus Ruta and Ruta. The height is 80cm, and the leaves are piled up in layers, split into several branches, and divided into small leaves. Flowers are formed with 4 to 5 yellow petals and bloom in June to September, and fruits are pointed in August to November. Distributed in Eurasia and North Africa.

Preferably, the skin whitening and anti-wrinkle composition of the present invention contains the sangolchwi extract, the sagebrush extract, the quinoa extract, and the callephensis rue extract, thereby improving skin whitening compared to the case where only the sangolchwi extract is included. And may exhibit wrinkle improvement effect, and may also exhibit anti-obesity activity in addition.

More preferably, it may contain 1 to 5 parts by weight of the sangolchwi extract, 1 to 5 parts by weight of the sangolchi extract, 1 to 5 parts by weight of the Orchid alfalfa extract, and 1 to 5 parts by weight of the rue extract.

When used as a complex extract within the range of parts by weight, a synergistic effect of critical significance is expressed due to the synergistic effect of the interaction of each extract, and by including it as a complex extract, the unique aroma or taste of the extract As a result, palatability can be improved. In addition, when out of the above range, the synergistic effect is rapidly reduced or almost absent.

The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohol, and mixtures thereof.

A method for preparing the extract may be a conventional extraction method in the art, such as an ultrasonic extraction method, a leaching method, and a reflux extraction method. Specifically, it may be an extract obtained by extracting a natural product from which foreign substances are removed by washing and drying with water, alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, and may be an extract obtained by sequentially applying the solvents to a sample.

In the ultrasonic extraction method, the reaction is performed at 30 to 50 ° C. for 0.5 to 2.5 hours, and the extraction solvent is water or 50 to 100% of alcohol having 1 to 6 carbon atoms. Specifically, extraction is performed at 40 to 50 ° C. for 1 to 2.5 hours, and water or 70 to 80% of alcohol having 1 to 6 carbon atoms is used as an extraction solvent.

The leaching method is carried out at 15 to 30 ° C. for 24 to 72 hours, and water or 50 to 100% of alcohol having 1 to 6 carbon atoms is used as an extraction solvent. More specifically, it is performed at 20 to 25 ° C. for 30 to 54 hours, and the extraction solvent is water or 70 to 80% of alcohol having 1 to 6 carbon atoms.

The reflux extraction method is based on 100 mL of water and alcohol having 1 to 6 carbon atoms, 10 to 30 g of pulverized natural product, 1 to 3 hours of reflux time, and 50 to 100% of alcohol having 1 to 6 carbon atoms or water. More specifically, based on 100 mL of alcohol having 1 to 6 carbon atoms or 100 mL of water, 10 to 20 g of pulverized natural product, 1 to 2 hours of reflux, and 70 to 90% of alcohol or water having 1 to 4 carbon atoms.

The extraction solvent may be used in an amount of 2 to 50 times, more specifically, 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for leaching in the extraction solvent for 1 to 72 hours, more specifically 24 to 48 hours.

After extraction, the extract can be fractionated by sequentially applying a fresh fractionation solvent. The fractionation solvent used in the fractionation is at least one selected from the group consisting of water, hexane, butanol, ethylacetic acid, ethylacetate, methylene chloride, and mixtures thereof, and is preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, a method such as concentration or lyophilization may be additionally used.

A cosmetic composition according to another embodiment of the present invention includes the composition for skin whitening and wrinkle improvement.

The cosmetic composition of the present invention may be prepared by mixing a dermatologically acceptable carrier in addition to the composition of the present invention.

The dermatologically acceptable carrier may include purified water, oil, wax, fatty acid, fatty alcohol, fatty acid ester, surfactant, moisture absorbent, thickener, antioxidant, viscosity stabilizer, chelating agent, buffer, preservative, lower alcohol, and the like. However, it is not limited thereto, and the type and concentration are various, and include parts that those skilled in the art can change within the scope of the present invention.

In addition, if necessary, functional substances such as whitening agents, moisturizing agents, anti-inflammatory agents, antibacterial agents, antifungal agents, vitamins, sunscreens, antibiotics, anti-acne agents, perfumes, and dyes may be included, which are generally used in the cosmetic field. As may be included in the cosmetic composition according to the present invention. The cosmetic composition of the present invention may contain at least one moisturizing active ingredient exhibiting the same or similar function in addition to the composition of the present invention in order to enhance its functional effect.

The cosmetic composition is a formulation selected from the group consisting of skin, serum, lotion, emulsion, cream, essence, lotion, foundation, pack, soap, surfactant-containing cleansing, spray and powder.

Cosmetics prepared from the cosmetic composition of the present invention can be prepared in the form of general emulsifier type and solubilization formulation. Emulsified cosmetics include nutrition lotion, cream, essence, etc., and solubilized cosmetics include softening lotion.

In addition to the composition of the present invention, the cosmetic may be prepared in the form of an adjuvant that can be applied topically or systemically, which is commonly used in the art, by containing a dermatologically acceptable medium or base.

Suitable cosmetic formulations include, for example, solutions, gels, solid or pasty anhydrous products, emulsions obtained by dispersing an oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), non-ionic forms. It may be provided in the form of a follicular dispersion, cream, toner, lotion, powder, ointment, spray or conceal stick. In addition, it can be prepared in the form of a foam (foam) or the form of an aerosol composition further containing a compressed propellant.

Products to which the cosmetic composition of the present invention can be added include, for example, cosmetics such as astringent lotion, softening lotion, nutrient lotion, various creams, essences, packs, foundations, etc., and cleansing, face wash, soap, treatment or beauty essence etc. Specific formulations include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, essence, nutrient essence, pack, soap, shampoo, and cleansing foam. , cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder, or eye shadow.

A food composition according to another embodiment of the present invention includes the composition for skin whitening and wrinkle improvement.

At this time, the amount of the extract in the food or beverage may be added at about 0.01 to 15% by weight of the total food weight, and the health drink composition may be added at a rate of about 0.01 to 10g based on 100 ml, but is not limited thereto .

When the food composition of the present invention is a beverage composition, there is no particular limitation on other ingredients except for containing the extract as an essential ingredient in the indicated ratio, and it may contain various flavoring agents or natural carbohydrates as additional ingredients like conventional beverages. can Examples of the aforementioned natural carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) may be used, but are not limited thereto. The ratio of the natural carbohydrates may generally range from about 1 to about 20 g per 100 ml of the composition of the present invention, but may not be limited thereto.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its It may contain salts, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like, but may not be limited thereto.

In addition, the composition of the present invention may contain natural fruit juice and fruit flesh for producing fruit juice beverages and vegetable beverages, and these components may be used independently or in combination. The ratio of these additives is not critical, but may be selected in the range of about 0.1 to about 20 parts by weight per 100 parts by weight of the composition of the present invention, but is not limited thereto.

In addition, it is possible to prepare a functional food including the food composition, and the functional food according to the present invention uses a specific ingredient as a raw material for the purpose of health supplementation, or extracts, concentrates, refines, mixes, etc. a specific ingredient contained in a food raw material. It is a food manufactured and processed by the method, including health supplement food, and is designed and processed so that other food components can fully exert their bioregulatory functions such as biodefense, regulation of biorhythm, prevention and recovery of disease, etc. It is intended to include all foods that also have functions related to disease prevention and recovery from diseases.

The composition of the present invention provides a natural extract-based composition capable of exhibiting skin whitening and wrinkle improvement activities through tyrosinase inhibition and elastase inhibition activity by including an extract of sangolchi as an active ingredient. can do.

The composition of the present invention may provide a composition capable of exhibiting an anti-obesity effect through the adipocyte formation inhibitory activity by including the extract of Sangolchi as an active ingredient.

In addition, by providing the composition of the present invention as a cosmetic composition or food composition, etc., it is possible to provide a composition that can exhibit excellent functionality and preference without causing side effects due to long-term use.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

[Preparation Example 1: Preparation of natural extract]

1. Preparation of wild bone extract

After washing the sangolchwi with water, it was completely air-dried. After pulverizing the dried sangolchwi with a blender, leaching with 80% ethanol at room temperature for 36 hours, the sample was filtered to prepare an extract (SE) of sangolch.

2. Preparation of other extracts

In the same manner as the mountain chrysanthemum extract (SE), a mortise stem extract (EE), a quinoa extract (CE), and a callephensis rue extract (RE) were prepared.

3. Preparation of complex extracts

A composite extract was prepared by mixing the sangolchwi extract (SE), the sagebrush extract (EE), the quinoa extract (CE), and the rue extract (RE) in the weight range shown in Table 1 below. .

MX1 MX2 MX3 MX4 MX5 MX6 MX7 MX8 MX9 SE 100 100 100 100 100 100 100 100 100 EE - 20 - - One 10 20 30 40 CE - - 20 - One 10 20 30 40 RE - - - 20 One 10 20 30 40

(unit: parts by weight)

[Experimental Example 1: Cytotoxicity evaluation of natural extract]

In order to evaluate the safety of the extracts of Sangolchi (SE, MX1), the cell viability of human epithelial cells was confirmed by MTT analysis. Specifically, HaCaT cells, a skin keratinocyte cell line, were inoculated into a 12-well plate at a concentration of 1×10 ⁴ cells/well, cultured for 24 hours, and then washed with PBS. Then, after adding samples of sangolchi extract (SE, MX1) diluted at each concentration (0, 20, 40, 60, 80, 100, 120 μg/ml) to each well (negative control group is untreated group ), cultured for 24 hours and 48 hours. After culturing for 24 hours and 48 hours, 40 μl of 0.5% MTT solution was added to each well, and cultured for 1 hour in a 5% CO ₂ , 37° C. incubator. After removing the culture medium, 150 μl of DMSO was added and shaken for 10 minutes. Then, the absorbance was measured at 595 nm using an ELISA plate reader. was found not to affect

In addition, in the same manner as above, the cytotoxicity test of natural extracts, such as the natural extracts, E. It was found that neither the Quercus quinoa extract (CE) nor the Calefensis rue extract (RE) treatment group had any significant effect on cell proliferation.

Accordingly, it can be confirmed that the treatment of the sangolchwi extract (SE), the sagebrush extract (EE), the quinoa extract (CE), and the rue extract (RE) of the present invention does not exhibit toxicity to cells.

[Experimental Example 2: Evaluation of Skin Improvement of Sangolchwi Extract]

1. Evaluation of skin whitening improvement

The inhibitory effect on tyrosinase activity was measured for the extracts (SE, MX1) obtained in Preparation Example 1. In this experiment, tyrosinase extracted from mushrooms was used (Sigma-Aldrich, US). An experimental method was used to measure the degree of color development based on the formation of melanin of the L-tyrosine substrate, which is a substrate for tyrosinase, by measuring the absorbance value at 490 nm. A reaction solution was prepared by adding 40 μl of 1.5 mM L-tyrosine to 120 μl of 0.1 M phosphate buffer (Ph 6.8). After adding 20 μl of sangolchi extract (SE, MX1) according to the concentration to the reaction solution and stabilizing for 5 minutes, 20 μl of mushroom tyrosinase (100 U/ml) was added and enzymatic reaction was performed at 37° C. for 10 minutes. In the positive control group, arbutin was used instead of the sample solution of Sangolchwi extract. After adding all the reaction solutions and enzymatically reacting at 37° C. for 10 minutes, the absorbance was measured at 490 nm and calculated according to Equation 1 below to obtain the degree of enzyme inhibitory activity of the sample. The results are shown in Table 2 below.

[Equation 1]

Tyrosinase activity inhibition rate (%) = [1 - {(absorbance of test group - absorbance of sample) / (absorbance of control group - absorbance of blank sample solution)}] X 100

extract Tyrosinase activity inhibition rate (%) 0 μg/mL 40 μg/mL 80 μg/mL 120 μg/mL mountain odor 0 11.69 23.34 30.01 Arbutin 0 10.02 18.77 23.57

(unit : %)

Referring to Table 2, as a result of evaluating the tyrosinase activity inhibition rate by treating the extract (SE, MX1) of the present invention for each concentration, it can be confirmed that the effect is similar to or higher than that of the positive control arbutin. , It can be confirmed through the experimental results that it exhibits excellent skin whitening activity in a concentration-dependent manner.

2. Evaluation of skin wrinkle improvement

Elastase inhibitory activity was measured in order to evaluate the effect of improving skin wrinkles with respect to the extract (SE, MX1) obtained in Preparation Example 1. The extract (SE, MX1) of Preparation Example 1 was diluted with 0.2 M Tris-Cl buffer (pH 8.0) for each concentration and used as an elastase substrate in 1 ml of 0.2 M Tris-Cl buffer (pH 8.0). 4 mg of N-succinyl-(Ala)3-p-nitroanilide was added to make 8.8 mM (substrate solution). Porcine pancreatic elastase (PPE) was used in a 0.2 M Tris-Cl buffer (pH 8.0) at a concentration of 10 ug/ml (enzyme solution).

After mixing 60 ul of diluted extract sample, 20 ul of substrate solution, and 20 ul of enzyme solution by concentration and reacting at 25 ° C. for 15 minutes, the absorbance was measured at 405 nm and elastase inhibitory activity (%) according to the following formula 2 was calculated. For the control group, a blank test group to which distilled water was added was used instead of the sample, and 1 mg/ml of adenosine was used as a positive control group. The results are shown in Table 3 below.

[Equation 2]

Elastase inhibitory activity (%) = {1-(B-C)/(A-D)} X 100

A = Absorbance after reaction by adding distilled water instead of sample and adding enzyme

B = absorbance of the sample after reaction with the addition of enzyme

C = absorbance of the sample after reaction by adding distilled water instead of enzyme

D = absorbance after reaction by adding distilled water instead of sample and enzyme

Treatment concentration (μg/ml) Elastase activity inhibition rate (%) Mountain bone extract (SE, MX1) One 21.89 4 41.42 8 59.75 12 67.11 Adenosine 1mg/mL 40.67

(unit : %)

Referring to Table 3, it can be seen that the elastase activity inhibition rate is improved in a concentration-dependent manner when the Sangolchi extract of the present invention is treated. It can be seen that the anti-wrinkle effect is excellent in the case of the extract (SE, MX1) of the present invention because the elastase activity inhibition rate is higher.

[Experimental Example 3: Evaluation of anti-obesity activity of Sangolchwi extract]

1. Evaluation of anti-obesity activity

In order to investigate the effect of the addition of the Sangolchi extract (SE, MX1) prepared in Preparation Example 1 on the formation of adipocytes in 3T3-L1 pre-adipocytes, differentiation of adipocytes was induced and only lipid droplets were specifically stained. The accumulation of lipid droplets in adipocytes was observed using the Oil red O method.

Lipid droplets are inactive vesicles composed of single-layer neutral fat surrounded by phospholipids that appear during the differentiation process from fibroblast precursors to adipocytes, and are regulated by important adipogenic transcription factors such as PPARγ. When lipid droplet is dyed by oil red O method, only neutral fat and cholesterol ester are dyed, and other free fatty acids and phospholipids are not dyed. Since most of the areas where lipid droplets are accumulated are neutral fat, only lipid droplets can be dyed. Therefore, in order to confirm the effect of sangolchi extract (SE, MX1) on the formation of lipid droplets that appear during the differentiation of 3T3-L1 preadipocytes into adipocytes, differentiation of 3T3-L1 cells was induced and then oil red o The formation of lipid droplets was observed under a phase contrast microscope at 10× magnification before and after staining. The results are shown numerically in Table 4 below.

division Inhibition rate of fat globules formation in mast cells (%) 0 μg/mL 40 μg/mL 80 μg/mL 120 μg/mL Mountain bone extract (SE, MX1) 0 41.22 70.81 72.49

(unit : %)

As a result, it was observed that in the experimental group in which only 3T3-L1 cell differentiation was induced without treatment with mountain bone extract (SE, MX1), lipid droplet accumulation was actively progressed and the formation of adipocytes in mast cells was not suppressed. When the extracts (SE, MX1) were treated, the formation of fat globules was greatly inhibited. It was confirmed that the inhibitory effect of lipid droplets was enhanced in a concentration-dependent manner with the treatment of Sangolchi extract.

Therefore, it can be seen that the extracts of the present invention (SE, MX1) have a great effect on suppressing obesity when ingested by reducing the accumulation of adipocytes in adipocytes.

[Experimental Example 4: Comprehensive evaluation of composite extract]

Improvement of skin whitening by a complex extract further comprising natural extracts of the mountain bone extract (MX1, SE) of the present invention; , In order to confirm through experiments whether wrinkle improvement and anti-obesity activity are increased, the effects of the complex extracts (MX1 to MX9) of Table 1 were compared in the same way as the experimental method of the Sangolchi extract shown in Experimental Example 2, and the following table 5 as a whole.

For objective comparison of the experimental results, the effect of the extracts of Sangolchi (MX1, SE) was evaluated as an index of 1 to 10 through relative comparison with an index of 5, and the higher the index, the better.

MX1
(SE) MX2 MX3 MX4 MX5 MX6 MX7 MX8 MX9 Inhibition of tyrosinase activity 5 5 6 6 4 8 8 9 4 Inhibition of elastase activity 5 6 6 5 3 8 9 9 4 Inhibition of fat globule formation in mast cells 5 6 5 6 4 8 8 8 4

(Unit: index)

According to Table 5, compared to MX1 (SE), which was treated with only the mountain chrysanthemum extract alone, the three natural extracts of the present invention, sagebrush extract (EE), quinoa spp. extract (CE), and callephensis rue extract (RE) were obtained. In the case of MX2 to MX4, each of which further includes tyrosinase activity, elastase activity, and inhibitory activity in fat globule formation in mast cells are slightly improved, it can be seen that the activity is improved by including the natural extract.

Furthermore, in the case of MX5 to MX9, which contains all of the natural extracts, the natural extracts, tuna extract (EE), C. quinoa extract (CE), and rue extract (RE), it can be confirmed that the activity is further improved according to the mixed use. In particular, 1 to 5 parts by weight of a parsnip extract (EE), 1 to 5 parts by weight of a quinoa extract (CE) and rue extract (RE) 1, based on 100 parts by weight of mountain bone extract (MX1, SE) In the case of MX6 to MX8 included in 5 to 5 parts by weight, the evaluation index corresponds to 8 to 9, so when included in a composition of a preferred weight part instead of simple mixing, skin whitening and skin wrinkle improvement activities are improved, so that the composition has excellent skin functionality. It was confirmed through experiments that it is possible to provide as a composition showing a complex function by adding an anti-obesity effect as well as possible.

[Experimental Example 5: Evaluation of preference for tea beverage]

Tea beverages containing MX1 to MX9 were prepared, and 30 adult male and female testers in their 20s to 60s tasted them, and then evaluated the aroma and taste to evaluate the preference on a scale of 1 to 10. After synthesizing and averaging the results, they are shown in Table 6 below. The higher the index below, the better the preference, and each index was expressed by applying rounding to the average value.

MX1 MX2 MX3 MX4 MX5 MX6 MX7 MX8 MX9 taste 2 3 3 4 5 8 9 9 6 incense 2 3 4 3 6 8 8 9 7 Synthesis
preference 2 3 4
4 6 8 9 9 7

(Unit: Index)

Referring to Table 6, compared to the case of the MX1 (SE) containing only the extract of the present invention, the natural extracts of the natural extract, the quince extract (EE), the quinoa extract (CE), and the raspberry extract (RE), respectively. In the case of M2 to M4 containing more, it can be confirmed that the preference for taste and aroma is improved,

It can be seen that in the case of M5 to M9 containing all of the above-mentioned Mortaraxe extract (EE), C. quinoa extract (CE) and Calephensis rue extract (RE), the acceptability is further improved.

In particular, in the case of M6 to M8 containing preferred parts by weight of a mortar and pestle extract (EE), a lynx elegans extract (CE), and a callephensis rue extract (RE), as the overall preference is further improved, a composition with high preference can be provided. it can be seen that there is

Therefore, it can be seen that in the case of the mixed formulation, it is possible to improve the degree of acceptability of the wild extract (MX1, SE) based on the natural extract, and through this, there are no problems such as side effects due to long-term use , It can be confirmed that it can be provided as a food composition and a cosmetic composition with excellent skin whitening and wrinkle improvement activity, and improved anti-obesity activity and excellent taste and flavor preference, as well as functionality. there is.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also made according to the present invention. falls within the scope of the rights of

Claims (7)

Containing mountain bone extract
A composition for skin whitening and wrinkle improvement. According to claim 1,
The composition exhibits skin whitening and anti-wrinkle activity by inhibiting tyrosinase and elastase.
A composition for skin whitening and wrinkle improvement. According to claim 1,
The composition exhibits anti-obesity activity by inhibiting the formation of adipocytes in adipocytes.
A composition for skin whitening and wrinkle improvement. According to claim 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols, and mixtures thereof.
A composition for skin whitening and wrinkle improvement. A composition for skin whitening and wrinkle improvement according to any one of claims 1 to 4
cosmetic composition. According to claim 5,
The cosmetic composition is a formulation selected from the group consisting of skin, serum, lotion, emulsion, cream, essence, lotion, foundation, pack, soap, surfactant-containing cleansing, spray and powder
cosmetic composition. Claims 1 to 4, comprising a composition for skin whitening and wrinkle improvement according to any one of claims 1 to 4
food composition.