KR20200124020A - Composition for skin whitening comprising Tradescantia pallida extract - Google Patents

Abstract

The present invention relates to a composition for skin whitening comprising a Tradescantia pallida extract as an active ingredient. According to the present invention, the Tradescantia pallida extract exhibits antioxidant activity and tyrosinase inhibitory activity to have an excellent skin whitening effect, and thus may be favorably used as a cosmetic composition for skin whitening, a pharmaceutical composition for skin whitening, a health product composition for skin whitening, and the like.

Description

Composition for skin whitening comprising Palidajaju chicken gaebi extract as an active ingredient {Composition for skin whitening comprising Tradescantia pallida extract}

The present invention relates to a composition for skin whitening comprising an extract of Palidajaju chicken gaebi as an active ingredient.

Skin is one of the major tissues that perform physical and biochemical defense functions of the human body, and is composed of epidermis, dermis, and hypodermis, and collagen produced from fibroblasts in the dermis ( There is an extracellular matrix (ECM) composed of collagen) and elastin, which regulates the elasticity of the skin and is involved in wrinkle formation. Collagen, which occupies more than 90% of the dermis, performs functions such as mechanical firmness of the skin, resistance of connective tissue and binding force of tissues, support of cell adhesion, induction of cell division and differentiation, and occupies about 2-4% of the dermal layer. The present elastin is an insoluble fibrous protein and performs the function of wrapping collagen bundles.

On the other hand, in modern society, skin such as erythema reactions, pigment reactions, skin aging, skin cancer, etc., due to the increase of the average life span, increased opportunities for UV exposure due to the increase in leisure activities, the destruction of the ozone layer due to environmental pollution, and the increase in the absolute amount of UV. Change is increasing. In particular, when exposed to UV for a long period of time, the stratum corneum of the skin becomes thick and the extracellular matrix, which is a component of the skin, is denatured, reducing the elasticity of the skin and causing abnormalities in the skin pigment, resulting in excessive production of melanin.

Melanin, which acts as a determinant of skin color, is a polymer compound of phomelanin or eumelanin, which is synthesized in organelles called melanosomes in melanocytes. The synthesized melanin pigment is delivered to adjacent cells, keratinocytes, through dendrites of melanocytes, and the delivered melanin pigments are distributed in various parts of the skin through the keratinocytes. The most important intracellular factor in melanin synthesis is a transcription factor called microphthalmia-associated transcription factor (MITF), which is responsible for increasing the expression of tyrosinase, which catalyzes the final melanogenesis process. do. Melanin, which is excessively produced by causes such as ultraviolet rays, hormones, and inflammation, deposits pigment on the skin to form spots and freckles, and may also cause skin cancer. Therefore, in order to maintain young and beautiful skin, it is necessary to develop a new whitening agent along with the development of antioxidants that can protect skin cells and tissues.

Meanwhile, Tradescantia pallida is a fleshy, evergreen sukkeun herb belonging to the family Chicken Epiaceae and is native to northeastern Mexico. The flower has 3 pink petals and yellow stamens, and the leaf stem is purple. It can be easily propagated through cuttings and is cultivated for ornamental purposes. As for the physiological activity of Palidajaju chicken gaebi, studies on the antioxidant activity and antibacterial activity of leaves collected in Malaysia have recently been reported, but there have been no related studies since it was introduced in Korea.

Korean Patent Registration No. 10-1152326 (registered on May 25, 2012)

An object of the present invention is to provide a cosmetic composition for skin whitening, a pharmaceutical composition for skin whitening, or a health food composition for skin whitening.

Another object of the present invention is to provide a reagent composition for inhibiting tyrosinase activity.

In order to achieve the above object, the present invention provides a cosmetic composition for skin whitening comprising an extract of Palidajaju chicken ( Tradescantia pallida ) as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for skin whitening comprising an extract of Palidajaju chicken ( Tradescantia pallida ) as an active ingredient.

In addition, the present invention provides a health food composition for skin whitening comprising an extract of Palidajaju chicken ( Tradescantia pallida ) as an active ingredient.

In addition, the present invention provides a reagent composition for inhibiting tyrosinase activity comprising an extract of Tradescantia pallida as an active ingredient.

According to the present invention, the Palidajaju chicken gaebi extract exhibits antioxidant activity and tyrosinase activity inhibitory activity, so it has excellent skin whitening effect, and is useful as a cosmetic composition for skin whitening, a pharmaceutical composition for skin whitening, a health food composition for skin whitening, etc. Can be utilized.

Figure 1 is a check of the total polyphenol content of the Palidajaju chicken gaebi extract.
Figure 2 is to confirm the total flavonoid content of the extract of Palidajaju chicken gaebi.
Figure 3 is to confirm the electron donating ability of Palidajaju chicken gaebi extract.
Figure 4 is to confirm the tyrosinase activity inhibitory ability of the extract of Pali dajaju chicken gaebi.

The inventors of the present invention prepared an extract of Palidajaju chicken gaebi using ethanol, methanol or ethyl acetate, and confirmed the skin whitening effect from the antioxidant activity and tyrosinase activity inhibition activity of the extract, and completed the present invention.

Accordingly, the present invention provides a cosmetic composition for skin whitening comprising an extract of Palidajaju chicken ( Tradescantia pallida ) as an active ingredient.

The extract may be extracted with any one or more solvents selected from the group consisting of ethanol, methanol, and ethyl acetate, but it is specified that the extract is not limited thereto.

It should be noted that the extract may be included in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total composition, but is not limited thereto.

The extract may improve pigmentation by inhibiting the activity of tyrosinase and exhibit a skin whitening effect.

When the composition of the present invention is a cosmetic composition, the cosmetic composition may include conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and fragrances, and a carrier in addition to the active ingredients. In addition, the cosmetic composition may further include a skin absorption accelerator to enhance its effect.

The formulation of the cosmetic composition may be prepared in any formulation conventionally prepared in the art, for example, the cosmetic composition is a lotion, emulsion, skin, toner, lotion, essence, sunscreen, cream, makeup base, foundation , Powder, pack, gel, ointment, patch, stick, shampoo, rinse, spray, makeup remover and detergent, etc. may be formulated, but is not limited thereto.

When the formulation is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component. .

When the formulation is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane/butane Or a propellant such as dimethyl ether.

When the formulation is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3 -Butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the above formulation is a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose , Aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

In addition, the present invention provides a pharmaceutical composition for skin whitening comprising an extract of Palidajaju chicken ( Tradescantia pallida ) as an active ingredient.

When the composition of the present invention is a pharmaceutical composition, for administration, it may include a pharmaceutically acceptable carrier, excipient, or diluent in addition to the above-described active ingredients. Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, Polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oils.

The pharmaceutical compositions of the present invention can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, or sterile injectable solutions according to a conventional method. . Specifically, when formulated, it may be prepared using diluents or excipients such as fillers, weight agents, binders, wetting agents, disintegrants, and surfactants that are commonly used. Solid preparations for oral administration include, but are not limited to, tablets, pills, powders, granules, capsules, and the like. Such a solid preparation may be prepared by mixing at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, gelatin, etc. in addition to the active ingredient. Further, in addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. It can be prepared by adding various excipients, such as wetting agents, sweetening agents, fragrances, preservatives, and the like, in addition to oral liquids and liquid paraffin. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations, and tasks. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyloleate, and the like may be used. As a base for suppositories, witepsol, macrosol, Tween 61, cacao butter, laurin, glycerogelatin, and the like can be used.

A suitable dosage of the pharmaceutical composition of the present invention varies depending on the condition and weight of the patient, the severity of the disease, the form of the drug, and the time, but may be appropriately selected by a person skilled in the art, and the daily dosage of the composition is preferably It is 0.001 mg/kg to 50 mg/kg, and it can be administered once to several times a day as needed.

In addition, the present invention provides a health food composition for skin whitening comprising an extract of Palidajaju chicken ( Tradescantia pallida ) as an active ingredient.

When the composition of the present invention is a health food composition, various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.), pectic acid and salts thereof , Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, and the like. In addition, it may contain pulp for the manufacture of natural fruit juices, synthetic fruit juices and vegetable beverages. These components may be used independently or in combination. In addition, the health food composition may be in the form of any one of meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, gum, ice cream, soup, beverage, tea, functional water, drink, alcohol and vitamin complex. I can.

In addition, the health food composition may additionally contain food additives, and the suitability as a food additive is determined according to the general rules and general test methods of the Food Additive Code approved by the Ministry of Food and Drug Safety, unless otherwise specified. It is judged according to standards and standards.

For example, ketones, glycine, potassium citrate, nicotinic acid, chemical synthetic products such as cinnamic acid, dark pigment, licorice extract, crystalline cellulose, high cooling pigment, natural additives such as guar gum, L-glutamic acid And mixed preparations such as sodium preparations, noodle-added alkali preparations, preservative preparations, and tar color preparations.

At this time, the composition according to the present invention added to the food in the process of manufacturing the health food composition can be appropriately added or subtracted, if necessary.

In addition, the present invention provides a reagent composition for inhibiting tyrosinase activity comprising an extract of Tradescantia pallida as an active ingredient.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example 1: Reagents and instruments

Phenol (Folin & Ciocalteu's phenol reagent), dimethyl sulfoxide (DMSO), diethylene glycol, potassium persulfate (potassium potroxodisulfate), 1,1-diphenyl-2-picrylhydrazyl (1,1-diphenyl-2-picrylhydrazyl; DPPH), tannic acid, rutin, tyrosine, and tyrosinase from mushroom are manufactured by Sigma (St. Louis, MO, USA). The product was used, and other general reagents used a special product.

Example 2: Palidajaju chicken gaebi extract preparation

To prepare the extract of Palidajaju chicken ( T. pallida ), the above-ground part of Palidajaju chicken gaebi growing in the glass greenhouse of the College of Life Science and Resources at Cheju National University was collected and dried in a dryer. The dried Palidajaju chicken gaebi was pulverized with a blender, filtered through a 500 μm sieve, extracted twice for 24 hours with 100 mL of ethanol, methanol or ethyl acetate per 10 g, and then concentrated with a reduced pressure concentrator. Samples that have not been completely concentrated are dried with a freeze dryer and sold separately by T. pallida ethanol extract (TPEE), T. pallida methanol extract (TPME), and ethyl acetate. An extract ( T. pallida ethyl acetate extract; TPAE) sample was prepared. Thereafter, the sample was dissolved in DMSO, diluted from 1000 μg/mL to 62.5 μg/mL with distilled water, and used in the experiment.

Example 3: Analysis of antioxidant activity

1) Analysis of total polyphenol content

Total polyphenol content was measured by the Folin-Denis method (Folin & Denis, 1912). 1 mL of folin-reagent was added to 1 mL of the sample diluted to a concentration of 200 μg/mL, reacted at room temperature for 3 minutes, 1 mL of 10% Na ₂ CO ₃ was added, and left at room temperature for 1 hour. Then, the absorbance was measured at 725 nm. The calibration curve was prepared using tannic acid.

As a result, as shown in FIG. 1, the total polyphenol content was 298.7 tannic acid equivalent (TAE) mg/g for TPEE, 418.4 TAE mg/g for TPME, and 430.1 TAE mg/g for TPAE.

2) Total flavonoid content

The total flavonoid content was measured by a variant of Davies et al. (1980). 1 mL of diethylene glycol was added to 100 μL of a sample diluted to a concentration of 200 μg/mL, reacted for 5 minutes at room temperature, and then 100 μL of a 1 N NaOH solution was added and left at 37°C for 1 hour. Then, the absorbance was measured at 420 nm. The calibration curve was prepared using a routine.

As a result, as shown in FIG. 2, it was confirmed that the total flavonoid content was 180.5 rutin equivalent (RE) mg/g for TPEE, 247.1 RE mg/g for TPME, and 359.1 RE mg/g for TPAE.

3) Electron donating ability (DPPH radical scavenging ability)

The electron donating ability was measured by the method of Blois (1958). To a 96-well plate, 50 μL of 0.2 mM DPPH solution dissolved in ethanol and 100 μL of Palidajaju chicken gaebi extract at 62.5, 125, 250, 500, and 1000 μg/mL concentrations were added each, and allowed to stand for 30 minutes in a light-shielded state. The absorbance was measured at nm. GraphPrism software Ver. 7 (Graphpad Software, lnc., La Jolla, CA, USA) was used to calculate SC ₅₀ (50% scavenging concentration, 50% free radical scavenging concentration).

As a result, as shown in Fig. 3, the Palidajaju chicken gaebi extract showed a positive (+) dose-response correlation in a concentration-dependent manner. It was confirmed that the electron donating ability was 97.5, 70.4, 66.7, and 91.9% at 1000 μg/mL, respectively. After calculating the _{SC 50, TPEE, TPME, SC} 50 for TPAE extract was 664.8, 660.8, was 277.3 μg / mL. The SC ₅₀ of AA was not calculated, but was found to be smaller than 125 μg/mL.

Example 4: Enzyme activity analysis

1) Mushroom tyrosinase activity inhibition

After mixing 5 μL of the sample diluted to 62.5, 125, 250, 500, 1000 μg/mL, 50 μL of sodium phosphate buffer (pH 6.8), 50 μL of 0.03% tyrosine, and 10 μL of mushroom tyrosinase. It was reacted at 37°C for 10 minutes. After cooling for 5 minutes on ice, the absorbance was measured at 475 nm. Arbutin was used as a positive control. The IC ₅₀ (50% inhibitory concentration, 50% inhibitory concentration of tyrosinase activity) was calculated using GraphPrism software.

As a result, as shown in Fig. 4, the Palidajaju chicken gaebi extract showed a positive (+) dose-response correlation in a concentration-dependent manner, and the mushroom tyrosinase activity inhibitory ability against the positive control arbutin and TPEE, TPME, and TPAE extracts was It was confirmed that the concentrations were 62.7, 66.0, 68.2, and 65.2% at 1000 μg/mL concentration, respectively. IC ₅₀ for arbutin, TPEE, TPME, and TPAE extracts were calculated as 437.7 287.7, 267.4 and 343.2 μg/mL, respectively. In other words, it was found that the Pali dajaju chicken gaebi extract was superior to the positive control, arbutin, to inhibit tyrosinase activity.

Therefore, from the above results, it was confirmed that the Palidajaju chicken gaebi extract can effectively inhibit skin pigmentation and exhibit a whitening effect.

As the specific parts of the present invention have been described in detail above, it is obvious that these specific techniques are only preferred embodiments, and the scope of the present invention is not limited thereto for those of ordinary skill in the art. Therefore, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be interpreted as being included in the scope of the present invention.

Claims (7)

A cosmetic composition for skin whitening comprising an extract of Palidajaju chicken ( Tradescantia pallida ) as an active ingredient. The cosmetic composition for skin whitening according to claim 1, wherein the extract is extracted with one or more solvents selected from the group consisting of ethanol, methanol, and ethyl acetate. The cosmetic composition for skin whitening according to claim 1, wherein the extract is contained in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total composition. The cosmetic composition for skin whitening according to claim 1, wherein the extract inhibits the activity of tyrosinase. A pharmaceutical composition for skin whitening comprising an extract of Palidajaju chicken ( Tradescantia pallida ) as an active ingredient. A health food composition for skin whitening comprising an extract of Palidajaju chicken ( Tradescantia pallida ) as an active ingredient. A reagent composition for inhibiting tyrosinase activity comprising an extract of Palidajaju chicken ( Tradescantia pallida ) as an active ingredient.

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