KR20230109286A - Composition for protecting photo-damaged skin comprising extract of Kadsura coccinea - Google Patents

Abstract

The present invention relates to a composition for skin photodamage protection comprising an extract of black oenophila, wherein the extract of each part of Kadsura coccinea, particularly the leaf extract of black oenophila, improves keratinocyte viability and releases LDH in the presence of UVA and UVB irradiation , It reduces intracellular ROS generation and cell death to show skin photodamage protection effects, so it can be used in various ways such as medicines for skin photodamage protection, cosmetics, and health functional foods.

Description

Composition for protecting photo-damaged skin comprising extract of Kadsura coccinea}

The present invention relates to a composition for protecting skin from photodamage comprising a black oatmeal extract.

Solar ultraviolet (UV), characterized by UVA (320-400 nm), UVB (280-320 nm), and UVC (100-280 nm), is the most important cause of skin cancer and photoaging due to cytotoxicity, genotoxicity and phototoxicity. is an environmental factor. Specifically, UVA and UVB account for more than 95% and more than 3% of daily UV irradiation, respectively, and UVA and UVB irradiation penetrate the epidermal and/or dermal layers of the skin to indirectly or directly induce reactive oxygen species (ROS). This can cause oxidative damage and cell death.

In recent decades, UV irradiation has become a serious skin health problem and is still spreading dangerously worldwide, and is known as a direct and consistent irritant to keratinocytes, which account for about 95% of the skin's epidermal cell mass. Keratinocytes act as the first barrier to microbial, chemical and physical hazards and help defend against UVA and UVB irradiation. It is known that when keratinocytes are exposed to UVA and UVB, intracellular ROS are generated to induce apoptosis.

On the other hand, Kadsura coccinea (Lem.) BC Sm) is an economically and medically important species belonging to Schizandra chinensis, also known as "black tiger" in China, and is mainly cultivated in southern China, Thailand, and Korea, and is used to prevent various diseases. became interested in Chinese folk remedies for In addition to being consumed as food, pharmacological properties, especially anti-HIV, anti-fungal, anti-lipid peroxidation, anti-hepatitis, anti-inflammatory and anti-tumor activity are known. In addition, many studies have confirmed therapeutic effects such as treating gastrointestinal disorders and rheumatoid arthritis, soothing the heart, strengthening the kidneys, and promoting blood and body fluid circulation.

However, the skin photoprotection effect using the black oenophile extract has not been known at all, and it is necessary to develop a new treatment for the defense of skin keratinocytes against UV irradiation that causes serious skin health problems.

Korean Registered Patent No. 10-2011240 (registered on Aug. 8, 2019)

An object of the present invention is to provide a composition for skin photoprotection through the development of a new therapeutic agent for the defense of skin keratinocytes against UV irradiation that causes serious skin health problems.

The present invention provides a pharmaceutical composition for protecting skin from photodamage comprising Kadsura coccinea extract.

In addition, the present invention provides a cosmetic composition for protecting skin from photodamage comprising Kadsura coccinea extract.

In addition, the present invention provides a health functional food for skin photodamage protection containing Kadsura coccinea extract.

In addition, the present invention provides an external preparation for skin photodamage protection containing Kadsura coccinea extract.

According to the present invention, extracts of black roe deer ( Kadsura coccinea ) parts, especially black roe leaf extracts, improve keratinocyte viability in the presence of UVA and UVB irradiation and reduce LDH release, intracellular ROS production and apoptosis, thereby reducing skin light Since it shows a damage protection effect, it can be used in various ways such as medicines for skin photodamage protection, cosmetics, and health functional foods.

Figure 1 shows the antioxidant effect of extracts of black roe deer parts, (A) pictures of each part of black roe deer, (B) total polyphenol content, (C) flavonoid content, (D) ABTS radical scavenging activity, and (E) DPPH radical scavenging activity, respectively.
Figure 2 shows the photodamage protection effect of the extract for each part of black oatmeal on the survival rate and cytotoxicity of keratinocytes by UVA or UVB irradiation.
Figure 3 shows the photodamage protection effect of extracts by parts of black oatmeal on ROS generation in keratinocytes by UVA or UVB irradiation.
Figure 4 shows the photodamage protection effect of the extract of each part of black oatmeal against apoptosis by UVA or UVB irradiation.

Hereinafter, the present invention will be described in more detail.

The inventors of the present invention have made diligent efforts to develop materials capable of preventing or treating skin photodamage, and as a result, extracts for each part of Kadsura coccinea , especially black roe leaf extracts, improve the viability of keratinocytes in the presence of UVA and UVB irradiation. The present invention was completed by revealing that the LDH release, intracellular ROS production and apoptosis were reduced to exhibit skin photodamage protection effects.

Accordingly, the present invention provides a pharmaceutical composition for skin photodamage protection containing Kadsura coccinea extract.

The black oenophile extract may be extracted from at least one of leaves, roots, stems, or fruits of black oenophile with water, C1 to C4 alcohol, or a mixed solution thereof, but is not limited thereto.

The black roe extract has a keratinocyte protective activity against ultraviolet rays. In particular, black roe extract has high total polyphenol and flavonoid content, shows radical scavenging activity, can improve keratinocyte viability in the presence of ultraviolet irradiation and reduce LDH release, intracellular ROS production and cell death, The skin photodamage protection effect of the black oenophile extract shows the excellent skin photodamage protection effect in the order of the black oko leaf extract, the black oko root extract, the black oko locust stem extract, and the black oko locust fruit extract.

The pharmaceutical composition may be provided in one or more formulations selected from the group consisting of gels, emulsions, injections, powders, granules, aerosols, pastes, percutaneous absorbents, and patches according to conventional methods, but is not limited thereto.

In another embodiment of the present invention, the pharmaceutical composition is a suitable carrier, excipient, disintegrant, sweetener, coating agent, expanding agent, lubricant, lubricant, flavoring agent, antioxidant, buffer, bacteriostatic agent commonly used in the preparation of pharmaceutical compositions , Diluents, dispersants, surfactants, binders and may further include one or more additives selected from the group consisting of lubricants.

Specifically, carriers, excipients and diluents are lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline Cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil may be used, and solid dosage forms for oral administration include tablets, pills, powders, granules, and capsules. These solid preparations may be prepared by mixing at least one or more excipients, for example, starch, calcium carbonate, sucrose or lactose, gelatin, etc., with the composition. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral administration include suspensions, solutions for oral use, emulsions, syrups, and the like, and various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included in addition to commonly used simple diluents such as water and liquid paraffin. Preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, suppositories, and the like. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base material of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogeratin and the like may be used.

The pharmaceutical composition is administered to a subject in a conventional manner through intravenous, intraarterial, intraperitoneal, intramuscular, intraarterial, intraperitoneal, intrasternal, transdermal, intranasal, inhalational, topical, rectal, oral, intraocular or intradermal routes. can be administered with

The preferred dosage of the black oenophile extract may vary depending on the condition and weight of the subject, the type and severity of the disease, the type of drug, the route and duration of administration, and may be appropriately selected by a person skilled in the art. According to one embodiment of the present invention, although not limited thereto, the daily dosage may be 1 to 1000 mg/kg, specifically 10 to 500 mg/kg. Administration may be administered once a day or divided into several administrations, and the scope of the present invention is not limited thereby.

In the present invention, the 'subject' may be a mammal including a human, but is not limited to these examples.

In addition, the present invention provides a cosmetic composition for protecting skin from photodamage comprising Kadsura coccinea extract.

The cosmetic composition according to the present invention can be prepared in any formulation conventionally manufactured in the art, skin lotion, skin softener, skin toner, astringent, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture It may be provided in a formulation selected from the group consisting of cream, hand cream, foundation, essence, nutrient essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser, but is not limited thereto.

The cosmetic composition may include a carrier acceptable in cosmetic formulations in addition to the black oenophile extract. The carrier may include alcohol, oil, surfactant, fatty acid, silicone oil, preservative, humectant, humectant, viscosity modifier, emulsion, stabilizer, sunscreen, coloring agent, fragrance, diluent, and the like. Specific compounds or compositions that can be used as the alcohol, oil, surfactant, fatty acid, silicone oil, preservative, humectant, humectant, viscosity modifier, emulsion, stabilizer, sunscreen, coloring agent, fragrance, diluent, etc. are already known in the art. Therefore, those skilled in the art can select and use an appropriate compound or composition.

In addition, the present invention provides a health functional food for skin photodamage protection containing Kadsura coccinea extract.

In the present invention, "functional health food" means a food manufactured and processed using raw materials or ingredients having useful functionality for the human body in accordance with the Health Functional Food Act, and "functional" refers to the structure and function of the human body. It refers to intake for the purpose of obtaining useful effects for health purposes such as regulating nutrients for functions or physiological functions.

The health functional food of the present invention may include conventional food additives, and the suitability as the "food additive" is determined according to the general rules of the food additive code approved by the Ministry of Food and Drug Safety and general test methods, etc., unless otherwise specified. It is judged according to the standards and standards for the relevant item.

Items listed in the "Food Additive Code" include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid, natural additives such as dark pigment, licorice extract, crystalline cellulose, goyang pigment, guar gum, and mixed preparations such as sodium L-glutamate preparations, noodle-added alkali preparations, preservative preparations, and tar color preparations.

The health functional food of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills and the like. For example, among the health functional foods in the form of capsules, hard capsules can be prepared by mixing and filling the composition according to the present invention with additives such as excipients in conventional hard capsules, and soft capsules contain the composition according to the present invention. It can be prepared by mixing with additives such as excipients and filling in a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a colorant, a preservative, and the like, if necessary. Definitions of terms for the excipients, binders, disintegrants, lubricants, corrigents, flavoring agents, etc. are described in literature known in the art, and include those having the same or similar functions. There is no particular limitation on the type of food, and it includes all health functional foods in the usual sense.

The health functional food can exhibit a more excellent skin photodamage protection effect by ingesting it in the form of inner beauty food. The inner beauty is an inner beauty food referred to as "eating cosmetics or beauty food", which refers to food that changes the skin constitution to be healthy by absorbing various ingredients good for the skin into the body, and cosmetics suitable for the skin type. You can select and consume inner beauty food that suits each individual by considering skin condition and lifestyle. More preferably, when the cosmetics containing the cosmetic composition and the inner beauty food containing the black roe extract are mixed, the skin photodamage protection effect is significantly increased compared to using only cosmetics, so that a more effective skin photodamage protection effect can be seen. has the advantage of

In the present invention, the term "prevention" refers to all activities that suppress or delay a disease by administering the composition according to the present invention. In the present invention, the term "treatment" refers to all activities that improve or beneficially change the symptoms of a disease by administering the composition according to the present invention. In the present invention, "improvement" means any action that improves the bad condition of a disease by administering or ingesting the composition of the present invention to a subject.

In addition, the present invention provides an external preparation for skin photodamage protection containing Kadsura coccinea extract.

The external preparation may be provided in a formulation selected from the group consisting of ointments, creams, gels, patches, sprays, warning agents, lotions, liniment agents, pasta agents, and cataplasma agents, but is not limited thereto.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for explaining the present invention in more detail, and it is to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. It will be self-evident.

<Example 1> Preparation of black roe extract

In a farm house in Ulsan, the conductivity of the nutrient solution of 3-year-old black roe in a 9L plastic pot is 1.0 mS ㅇ cm ^-1 , and the inorganic components [NO ₃ -N, 16; NH ₄ -N, 1.34; P, 4; K, 8; Ca, 8; and S, 4; Unit: me·L ^-1 ] was sufficiently watered using a complete nutrient solution. Black roe grown in pots was harvested in December 2020 by dividing into roots, stems, leaves, and fruits (Fig. 1A). The collected samples were immediately lyophilized in a lyophilizer and stored in plastic bags at -20 ° C until analysis. 20 g of each of the dried roots, stems, leaves and fruits of black oatmeal were pulverized into a fine powder and extracted with ethyl alcohol (EtOH) at room temperature. Filtration and evaporation of the EtOH extract of black oatmeal were carried out at 45° C. under reduced pressure, followed by lyophilization. Finally, the solid extract (50 mg/mL) was dissolved in dimethyl sulfoxide (DMSO) for further experiments.

<Example 2> Analysis of total phenolic and flavonoid content of black ocher extract

The total polyphenol and flavonoid contents of the extracts of the roots, stems, leaves and fruits of Black Rooster prepared in Example 1 were measured using Folin-Ciocalteu (total polyphenols) and aluminum chloride (flavonoids) colorimetric methods. Absorbance was measured at 700 nm (total polyphenols) using an Ultrospec 6300 Pro (GE Healthcare Life Sciences, Buckinghamshire, UK) and at 510 nm (flavonoids) using a VICTOR Multilabel Plate Reader (Perkin-Elmer, Waltham, MA, USA). ) was measured.

A standard curve was prepared using gallic acid (total polyphenols) and quercetin (flavonoids) as standards. QE/g).

As a result of analyzing the total phenol content, as shown in FIG. 1B, the black roe deer root extract (197.6 ± 27.2 mg GAE / g) showed the highest phenol content, and the black roe deer leaf extract (153.7 ± 6.7 mg GAE / g) and the black roe deer stem extract ( 88.1 ± 7.8 mg GAE/g) followed, followed by black roe fruit extract (21.8 ± 4.8 mg GAE/g) with the lowest phenol content.

In addition, as a result of flavonoid content analysis, as shown in FIG. 1C, the black roe deer leaf extract (94.5 ± 6.3 mg QE / g) showed the highest flavonoid content, followed by the black roe deer root extract (79.6 ± 4.2 mg QE / g), Black oenophila stem extract (14.9 ± 1.3 mg QE/g) and black oenophile fruit extract (6.4 ± 2.0 mg QE/g) had the lowest flavonoid content.

<Example 3> DPPH and ABTS analysis

The DPPH and ABTS radical scavenging activities of the extracts (0.5 mg/mL) of the roots, stems, leaves and fruits of black roe deer prepared in Example 1 were measured according to a previously known method [Nanomaterials 2020, 10, 1350] with slight modifications. Black roe root, stem, leaf and fruit extracts (0.5 mg/mL) were mixed with DPPH solution (60 μM) in a microplate. After shaking the sample vigorously, it was stored in the dark at 25°C for 30 minutes. A stock solution of 7 mM ABTS and 2.6 mM potassium persulfate was prepared in distilled water at room temperature in the dark for 18 hours. Extracts (0.5 mg/mL) of roots, stems, leaves, and fruits of black oatmeal were mixed with the above-prepared solution, and then left in the dark at room temperature for 30 minutes. The absorbance of the sample reactants was monitored at 510 nm (DPPH) or 734 nm (ABTS).

As shown in Figure 1D, the black roe leaf extract (94.7 ± 2.9%) and black roe root extract (82.8 ± 5.9%) showed the highest ABTS radical scavenging activity, and the black roe stem extract (29.7 ± 2.0%) and black roe roe fruit Extract (15.9±2.0%) followed. As shown in Figure 1E, the DPPH radical scavenging activity of black roe deer leaf extract (99.9 ± 0.1%) > black roe deer root extract (95.5 ± 3.6%) > black roe deer stem extract (25.7 ± 2.1%) > black roe deer fruit extract (8.7 ± 1.1%) %) in order.

<Example 4> Examination of the skin photoprotection effect of the black oatmeal extract

1. Cell culture

Adherent keratinocytes (HaCaT) were cultured in DMEM (Invitrogen Corporation, Carlsbad, CA, USA) containing 10% FBS (Invitrogen Corporation, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Invitrogen Corporation, Carlsbad, CA, USA). ) and inoculated into the medium solution, and incubated at 37°C with 5% CO ₂ . The growth of adherent HaCaT cells was observed regularly, and the medium was changed every 2-3 days. Cells in logarithmic growth phase were used for subsequent experiments.

2. UVA and UVB irradiation

Keratinocytes were irradiated with UVA or UVB (Bio-Link BLX-365, Villber-Lourmat, Eberhardzell, Germany), the UVA dose was 20 J/cm ² and the UVB dose was 50 mJ/cm ² .

3. Cell viability and cytotoxicity assay

For cell viability assay, Cell Counting Kit-8 (CCK-8) solution from CCK-8 Assay Kit (Sigma-Aldrich, St. Louis, MO, USA) was added to the HaCaT keratinocyte suspension according to the manufacturer's instructions and incubated at 37 °C. incubated for 4 hours. That is, 2x10 ⁴ cells were dispensed into each well of a 24-well plate and cultured for 24 hours at 37, 5% CO ₂ . After 24 hours of incubation, CCK-8 reagent was added to each well and cells were further cultured for 4 hours. Extracellular lactate dehydrogenase (LDH) release was measured in HaCaT keratinocyte culture medium using a cytotoxicity detection kit (Roche Applied Science, Switzerland). Absorbance was analyzed at 450 nm (CCK-8) and 490 nm (LDH) using a VICTOR Multilabel Plate Reader.

As a result of CCK-8 analysis, as shown in FIG. 2A, it was found that the black roe extract did not significantly change keratinocyte viability at a concentration of 0.5 mg/mL, and significantly inhibited keratinocyte viability in the presence of UVA or UVB irradiation. The viability of keratinocytes was increased by the treatment of extracts from each part of black oatmeal.

In addition, as a result of measuring extracellular LDH release, UVA or UVB irradiation significantly promoted LDH release as shown in FIG.

From these results, it was confirmed that the anti-proliferative and cytotoxic effects induced by UVA or UVB irradiation on keratinocytes were alleviated in the order of black roe leaf extract, black roe deer root extract, black roe deer stem extract, and black roe deer fruit extract. In particular, the black oenophile leaf extract was able to restore cell viability to a controlled level.

4. Assessment of intracellular ROS production in keratinocytes

Intracellular ROS levels were measured by 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate acetyl ester (CM-H ₂ DCFDA; Thermo Fisher Scientific, Waltham, MA, USA). analyzed using All procedures were performed according to the manufacturer's instructions. That is, after treatment with the extract (0.5 mg/mL) of each part of black oenophile, the keratinocytes (HaCaT) were washed with phosphate buffered saline (PBS) and incubated with CM-H ₂ DCFDA (5 μM) for 30 minutes in the dark. . Then, the production of intracellular ROS was visualized using a Carl Zeiss fluorescence microscope. Fluorescence intensity was measured based on a fluorescent dye (CM-H ₂ DCFDA) using a flow cytometer (Fit NXT Flow Cyto, Thermo Fisher Scientific, Pasadena, CA, USA).

As a result of fluorescence microscopic analysis, as shown in FIG. 3A, significant staining was shown in keratinocytes by UVA or UVB irradiation, but it was confirmed that the intracellular ROS level was suppressed by treatment with extracts of each part of black oatmeal.

According to the quantified results of flow cytometry, as shown in FIG. 3B, UVA and UVB irradiation increased the intracellular ROS level of keratinocytes by 27.2 ± 4.5% and 34.1 ± 4.2%, respectively, compared to the control group (5.7 ± 0.2%), and It was confirmed that the intracellular ROS level was inhibited in the order of leaf extract, black oenophila root extract, black oenophile stem extract, and black oko fruit extract.

From these results, it is shown that the black oenophila extract, in particular, the black oenophile leaf extract, significantly inhibited keratinocyte damage by reducing the level of endogenous ROS.

5. Apoptosis assay

After UV exposure and black roe extract treatment, HaCaT keratinocytes were trypsinized and centrifuged, and then fluorescein isothiocyanate (FITC) Annexin V/Dead Cell Apoptosis Kit (Invitrogen Life Technologies, Carlsbad, CA, USA) was used according to the manufacturer's instructions. Apoptosis of the obtained cells was evaluated. That is, keratinocytes were rinsed twice with PBS, and keratinocytes were obtained in annexin binding buffer and mixed with FITC/Annexin V (component A) and propidium iodide (PI) working solution. After incubation in the dark at room temperature for 15 minutes, keratinocyte apoptosis was measured and the percentage of apoptotic cells was calculated using a flow cytometer (Fit NXT Flow Cyto, Thermo Fisher Scientific, Pasadena, CA, USA). Signals for the FL1 (FITC/Nexin V) and FL3 (PI) channels were detected and quadrant marker staining and dot plots of the stained cells were set up.

As shown in FIG. 4A, UVA and UVB irradiation promoted Annexin V staining activity, whereas extracts from parts of black oatmeal reduced the Annexin V staining activity rate according to UVA and UVB irradiation. Annexin V staining activity was not induced by treatment with the extract alone (0.5 mg/mL) from each part of the black roe deer without UVA and UVB irradiation.

As shown in Figure 4B, the quantified data of flow cytometry showed that UVA and UVB irradiation increased the apoptosis level of keratinocytes by 46.3 ± 1.5% and 48.7 ± 1.0%, respectively, compared to the control group (5.0% ± 0.7%), but neither UVA nor UVB It was confirmed that the level of apoptosis was suppressed in the order of black roe leaf extract, black roe root extract, black roe stem extract, and black roe fruit extract by treatment with extracts of each part of black roe deer under irradiation conditions.

Hereinafter, formulation examples of a composition containing the black roe deer leaf extract (KCL) according to the present invention will be described, but the present invention is not intended to limit this, but only to be specifically described.

<Formulation Example 1> Prescription example of pharmaceutical composition

<Formulation Example 1-1> Preparation of powder

A powder was prepared by mixing 20 mg of KCL, 100 mg of lactose, and 10 mg of talc and filling in an airtight bag.

<Formulation Example 1-2> Manufacture of tablets

Tablets were prepared by mixing 10 mg of KCL, 100 mg of corn starch, 100 mg of lactose, and 2 mg of magnesium stearate, and then tableting according to a conventional tablet manufacturing method.

<Formulation Example 1-3> Preparation of capsule formulation

After mixing 10 mg of KCL, 100 mg of corn starch, 100 mg of lactose, and 2 mg of magnesium stearate, the above ingredients were mixed according to a conventional capsule preparation method and filled into gelatin capsules to prepare capsules.

<Formulation Example 1-4> Preparation of injection

After mixing 10 mg of KCL, an appropriate amount of sterilized distilled water for injection, and an appropriate amount of a pH adjusting agent, the above ingredient content was prepared per 1 ampoule (2 ml) according to a conventional method for preparing an injection.

<Formulation Example 2> Health Supplement

<Formulation Example 2-1> Manufacture of health food

KCL 1 mg, appropriate amount of vitamin mixture (vitamin A acetate 70 μg, vitamin E 1.0 mg, vitamin B1 0.13 mg, vitamin B2 0.15 mg, vitamin B6 0.5 mg, vitamin B12 0.2 μg, vitamin C 10 mg, biotin 10 μg, nicotinamide 1.7 mg, folic acid 50 μg, calcium pantothenate 0.5 mg) and appropriate amount of mineral mixture (ferrous sulfate 1.75 mg, zinc oxide 0.82 mg, magnesium carbonate 25.3 mg, monopotassium phosphate 15 mg, dicalcium phosphate 55 mg, potassium citrate 90 mg, calcium carbonate 100 mg, and magnesium chloride 24.8 mg) were mixed, then granules were prepared and health food was prepared according to a conventional method.

<Formulation Example 2-2> Manufacture of health drink

Add 1 mg of KCL, 1000 mg of citric acid, 100 g of oligosaccharide, 2 g of plum concentrate, 1 g of taurine, and purified water to make a total of 900 ml. After heating while stirring at 85 ° C., the resulting solution was filtered and collected in a sterilized 2 L container, sealed and sterilized, and stored in a refrigerator.

<Formulation Example 3> Cosmetic composition

<Formulation Example 3-1> Manufacture of skin lotion

3.0 parts by weight of propylene glycol, 0.1 part by weight of carboxypolymer, a small amount of preservative and the remaining amount of purified water are heated to 80 to 85 ° C. while mixing and stirring, and then introduced into the manufacturing unit, and then an emulsifier is operated, and polysorbate 60 1.0 part by weight, sorbitan Sesquioleate 0.5 parts by weight, liquid paraffin 10.0 parts by weight, sorbitan stearate 1.0 parts by weight, lipophilic monostearate glycerin 0.5 parts by weight, stearic acid 1.5 parts by weight, glyceryl stearate / PEG-400 stearate 1.0 parts by weight, triethanol After adding 0.2 parts by weight of amine by heating to 80 to 85 ℃, it was emulsified. After emulsification was completed, heat-cooled to 50 ° C while stirring using a stirrer, then added a trace amount of fragrance, cooled to 45 ° C, added a trace amount of pigment, added KCL at 35 ° C, cooled to 25 ° C, and aged.

<Formulation Example 2-2> Preparation of nutrient cream

0.3 parts by weight of carboxypolymer, 5.0 parts by weight of butylene glycol, 3.0 parts by weight of glycerin, and the remaining amount of purified water were heated to 80 to 85 ° C. while mixing and stirring, and then introduced into the manufacturing unit, and then an emulsifier was applied, and 2.0 parts by weight of stearic acid and cetyl alcohol 2.0 parts by weight, 2.0 parts by weight of glyceryl monostearate, 0.5 parts by weight of polyoxyethylene sorbitan monostearate, 0.5 parts by weight of sorbitan sesquioleate, glyceryl monostearate/glyceryl stearate/polyoxyethylene stearate 1.0 parts by weight of wax, 1.0 parts by weight of wax, 4.0 parts by weight of liquid paraffin, 4.0 parts by weight of squalane, and 4.0 parts by weight of caprylic/capric triglyceride were added by heating to 80 to 85° C., followed by emulsification by adding 0.5 parts by weight of triethanolamine. . After emulsification was completed, the mixture was cooled to 35 ° C while stirring using a stirrer, and then cooled to 25 ° C by introducing KCL and aged.

Having described specific parts of the present invention in detail above, it will be clear to those skilled in the art that these specific descriptions are merely preferred embodiments, and the scope of the present invention is not limited thereby. will be. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (10)

A pharmaceutical composition for skin photodamage protection comprising Kadsura coccinea extract. The pharmaceutical composition for protecting against photodamage to skin according to claim 1, wherein the extract of the black roe deer is obtained by extracting the black roe deer with water, C1-C4 alcohol, or a mixture thereof. The pharmaceutical composition for protecting against photodamage to the skin according to claim 1, wherein the black oatmeal is a leaf, root, stem or fruit. The pharmaceutical composition for protecting against photodamage to the skin according to claim 1, wherein the black roe extract has a keratinocyte protective activity against ultraviolet rays. A cosmetic composition for skin photodamage protection containing Kadsura coccinea extract. The method according to claim 5, wherein the cosmetic composition is skin lotion, skin softener, skin toner, astringent, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, foundation, essence, nutrient essence, pack, A cosmetic composition characterized in that it has a formulation selected from the group consisting of soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser. Health functional food for skin photodamage protection containing Kadsura coccinea extract. The health functional food according to claim 7, wherein the health functional food is an inner beauty food. An external preparation for skin photodamage protection containing Kadsura coccinea extract. The external preparation according to claim 9, characterized in that it is provided in a dosage form selected from the group consisting of ointments, creams, gels, patches, sprays, warning agents, lotions, liniment agents, pasta agents, and cataplasma agents.