KR102214765B1 - Cosmetic composition for skin whitening and anti-wrinkle containing extracts of herb mixture - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening and wrinkle improvement comprising as an active ingredient at least one extract selected from the group consisting of cornus milk, safflower, purslane and white dandelion.

Description

A cosmetic composition for skin whitening and wrinkle improvement containing natural compound extracts {COSMETIC COMPOSITION FOR SKIN WHITENING AND ANTI-WRINKLE CONTAINING EXTRACTS OF HERB MIXTURE}

The present invention relates to a cosmetic composition for skin whitening and wrinkle improvement comprising as an active ingredient at least one extract selected from the group consisting of cornus milk, safflower, purslane and white dandelion.

The causes of wrinkles can be broadly divided into wrinkles caused by natural aging and wrinkles caused by UV exposure (photoaging). Common phenomena found in relation to skin changes due to natural aging and photoaging are the reduction and transformation of collagen and elastin, the main constituent proteins in the dermis, and reduction of skin elasticity due to the reduction of hyaluronic acid, the matrix of the dermis. In particular, according to the results of the studies so far, it is known that the reduction and transformation of collagen and elastin, the main proteins in the dermis, are directly involved in the formation of wrinkles and the reduction of elasticity of the skin.

Human skin color is determined by several factors, such as the distribution of active blood vessels of melanocytes, which make melanocytes, the thickness of the skin, and the presence or absence of pigments inside and outside the body such as carotenoids and bilirubin. Among them, tyrosinase at melanocytes, etc. The most important is melanin, a black pigment produced by the action of several enzymes. The main function of melanin is to protect the skin from damage by removing many harmful radicals. Therefore, a high amount of melanin means that you have an effective countermeasure system that can protect your skin from physical or chemical toxins. Factors that promote the production of melanin include sunlight and ultraviolet rays, as well as estrogen or prostagladin. Melanin biosynthesis is a continuous oxidation reaction of specific enzymes such as tyrosinase, tyrosinase related protein1 (TRP-1), and dopachrome tautomerase (DCT) from the melanocytes of melanocytes in the epidermis Among them, the ability to inhibit tyrosinase activity, a major enzyme in melanin synthesis, plays an important role in the development of melanin polymer inhibitors.

Korean Patent Publication No. 10-2012-0076706

The present inventors completed the present invention by selecting cornus, safflower, purslane and white dandelion as natural substances having whitening and skin wrinkle improvement effects, and confirming the tyrosinase inhibitory effect of the complex extracts thereof.

In order to achieve the above object, the present invention provides a cosmetic composition for skin whitening and wrinkle improvement comprising one or more extracts selected from the group consisting of cornus, safflower, purslane and white dandelion as an active ingredient.

In one embodiment of the present invention, the composition may include white dandelion and safflower extract.

In one embodiment of the present invention, the composition may include cornus milk and safflower extract.

In one embodiment of the present invention, the composition may include cornus milk and purslane extract.

In an embodiment of the present invention, the absolut may be included in the range of 1 to 200 μg/mL.

In one embodiment of the present invention, the composition may inhibit tyrosinase.

In one embodiment of the present invention, the extract may be extracted using water or an organic solvent, and the organic solvent is methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene. It may be one selected from the group consisting of chloride, hexane and cyclohexane.

The natural product complex extract of the present invention not only exhibits an excellent antioxidant effect, but also exhibits an excellent synergistic effect in inhibiting tyrosinase, thereby having excellent effects in skin whitening and wrinkle improvement.

1 is a graph showing the total polyphenol content of cornus milk, safflower, purslane and white dandelion extracts according to an embodiment of the present invention.
2 is a graph showing the total flavonoid content of cornus milk, safflower, purslane and white dandelion extracts according to an embodiment of the present invention.
3 is a graph showing the DPPH radical scavenging ability of cornus milk, safflower, purslane and white dandelion extracts in an embodiment of the present invention.
4 is a graph showing the ABTS radical scavenging ability of cornus milk, safflower, purslane and white dandelion extracts in an embodiment of the present invention.
5 is a graph showing the tyrosinase inhibitory effect of cornus milk, safflower, purslane and white dandelion extracts and complex extracts thereof in an embodiment of the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description, detailed descriptions of techniques well known to those skilled in the art may be omitted. In addition, in describing the present invention, when it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description may be omitted. In addition, terms used in the present specification are terms used to properly express preferred embodiments of the present invention, which may vary depending on the intention of users or operators, or customs in the field to which the present invention belongs.

Therefore, the definitions of these terms should be made based on the contents throughout this specification. Throughout the specification, when a part "includes" a certain component, it means that other components may be further included rather than excluding other components unless specifically stated to the contrary.

The present invention relates to a cosmetic composition for skin whitening and wrinkle improvement comprising as an active ingredient at least one extract selected from the group consisting of cornus milk, safflower, purslane and white dandelion.

Cornus fructus is a dried fruit flesh by removing seeds from the fruit of Cornus officinalis , and has been used as a medicinal plant from ancient times, and is known to be good for liver disease and nerves and has anticancer activity. Constituents of cornus milk include gallic acid, malic acid, ursolic acid, and iridoid glycosides such as loganine and sweroside, and have been reported to have excellent antioxidant and anti-inflammatory effects.

Safflower ( Carthamus tinctorius ) is a one-year herb belonging to the Asteraceae family of the bellflower. Safflower seeds are rich in linoleic acid and vegetable sterols, and contain polyphenol compounds such as serotonin derivatives and lignans, so they have antioxidant activity, cholesterol-lowering action, anticancer, anti-cancer, It is known to have the effect of inflammation.

Purslane ( Portulaca oleracea ) is an annual plant in the family Purslane, and has been used as a skin soothing agent because it is effective in relieving swelling in folk remedies. Clinically, it has been used to treat wounds and diseases such as skin ulcers and dermatitis. Purslane contains ingredients such as coumarin, flavonoids and vitamin C, so it has excellent antioxidant activity, and is known to have various effects such as anticancer and anti-inflammatory.

Taraxacum coreanum is a native dandelion that grows in the lowlands of Korea and has long been used as a medicinal plant. White dandelion contains phenolic compounds such as taraxasterol and stigmasterol and flovonoid derivatives, and is known to have anti-inflammatory, antioxidant, immune-related activities, and anti-cancer effects.

The composition may be a complex extract of two natural products selected from cornus milk, safflower, purslane and white dandelion. The complex extract may be, for example, white dandelion and safflower extract, cornus and safflower extract, or cornus and purslane extract. The complex extracts showed a tyrosinase inhibitory activity that was increased by about 2.5 to 3.5 times compared to when used alone.

The extract may be extracted using water or an organic solvent, and the organic solvent may be, for example, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, and cyclo It may be selected from the group consisting of hexane. The organic solvent extraction method may include all methods that can be modified by one of ordinary skill in the art.

The composition may contain the complex extract alone, or may further include one or more pharmaceutically acceptable carriers, excipients, or diluents.

Cornus, safflower, purslane or white dandelion extract in the composition of the present invention may be included in a concentration of 1 to 200 μg/mL, and in the case of a complex extract of two or more natural products, each may be included in a mass ratio of 1:1.

In the above, the term "pharmaceutically effective amount" refers to an amount sufficient for the physiologically active ingredient to be administered to an animal or human to exhibit a desired physiological or pharmacological activity. However, the pharmaceutically effective amount may be appropriately changed according to the severity of symptoms, age, weight, health condition, sex, administration route, and treatment period of the patient.

In addition, "pharmaceutically acceptable" as used herein refers to physiologically acceptable and when administered to a human, usually does not cause allergic reactions such as gastrointestinal disorders, dizziness, or similar reactions. Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, Polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oils. In addition, fillers, anti-aggregating agents, lubricants, wetting agents, flavoring agents, emulsifying agents and preservatives may additionally be included.

In addition, the composition of the present invention may be formulated using methods known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal, and various for oral or parenteral administration. It can be formulated in a form. The formulation may be in the form of powders, granules, tablets, emulsions, syrups, aerosols, soft or hard gelatin capsules, sterile injectable solutions, sterile powders, ointments, lotions, creams, plasters, pastes, and the like.

The composition according to the present invention may be administered through various routes including oral, transdermal, subcutaneous, intravenous or intramuscular, and the dosage of the active ingredient may include various factors such as the route of administration, the patient's age, sex, weight, and the patient's severity. It can be appropriately selected according to. In addition, the composition of the present invention can be administered in parallel with a known compound capable of enhancing the desired effect.

Furthermore, the composition according to the present invention can be used not only as a cosmetic composition as described above, but also as a health functional food. For example, it can be easily utilized as a main raw material, an auxiliary raw material, a food additive, a functional food or a beverage.

The term "food" means a natural product or processed product containing one or more nutrients, and preferably means a state that can be eaten directly through a certain degree of processing, and as a general meaning, food , Food additives, functional foods and beverages.

Foods to which the composition for food may be added include, for example, various foods, beverages, gum, tea, vitamin complexes, and functional foods. In addition, special nutritional foods (e.g., formula, infant food, etc.), processed meat products, fish meat products, tofu, jelly, noodles (e.g., ramen, noodles, etc.), bread, health supplements, seasoning foods (e.g., soy sauce) , Soybean paste, red pepper paste, mixed sauce), sauces, confectionery (e.g. snacks), candy, chocolate, gum, ice cream, dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, pickles (various kimchi) , Pickles, etc.), drinks (eg, fruit drinks, vegetable drinks, soy milk, fermented drinks, etc.), natural seasonings (eg, ramen soup, etc.), but are not limited thereto. The food, beverage or food additive may be prepared by a conventional manufacturing method.

In addition, the term "functional food" or "health-functional food" refers to a food group or food composition that has added value to the food to act and express the function of the food for a specific purpose by using physical, biochemical, and bioengineering techniques. It refers to a food that is designed and processed to sufficiently express the body's control functions related to defense rhythm control, disease prevention and recovery, etc. to a living body, and specifically, may be a health functional food. The functional food may contain food additives acceptable food additives, and may further include suitable carriers, excipients, and diluents commonly used in the manufacture of functional foods.

The type of health supplement food is not limited thereto, but may be in the form of powder, granules, tablets, capsules, or beverages.

Advantages and features of the present invention, and a method of achieving them will become apparent with reference to embodiments described below in detail. Hereinafter, the present invention will be described in detail by examples. However, these examples are intended to specifically illustrate the present invention, and the scope of the present invention is not limited to these examples.

[Example]

Preparation Example 1: Preparation of natural product extract

Dry powders of cornus milk ( C. officinalis ), safflower ( C. tinctorius ), purslane ( P. oleracea ) and white dandelion ( T. coreanum ) were quantified, put in each flask, and immersed in 95% ethanol for 24 hours at room temperature. Left unattended. Then, the supernatant was taken and passed through a filter paper, and the filtrate was concentrated under reduced pressure. After measuring the weight of each extract, each yield was calculated (Table 1).

Example 1. Analysis of total polyphenol and flavonoid content

In order to measure the total polyphenol content of the extract of Preparation Example 1, the concentration of each extract was adjusted to 100 µg/ml. The total polyphenol content was diluted with 100 µl of each ethanol extract in 4.5 ml with distilled water, Folin-Ciocalteau) reagent 100 µl was added and left for 3 minutes. Then, 300 µl of 2% sodium carbonate (Na ₂ CO ₃ ) was added and allowed to stand at room temperature for 2 hours, and the absorbance was measured at 760 nm. The total polyphenol content was calculated from the calibration curve prepared by analyzing the same method as the sample by setting gallic acid as a standard material.

In order to measure the total flavonoid content of the extract of Preparation Example 1, the concentration of each extract was adjusted to a final concentration of 100 µg/ml. The total flavonoid content was 15 at room temperature by mixing 150 µl of each ethanol extract and 150 µl of 2% AlCl ₃ After reacting for a minute, absorbance was measured at 450 nm. The total polaronoid content was calculated from a standard curve prepared using quercetin.

The results of analyzing the total polyphenol and flavonoid contents of natural products are shown in FIGS. 1 and 2. As can be seen in Figures 1 and 2, it was confirmed that the extracts of cornus milk ( C. officinalis ) and purslane ( P. oleracea ) had a high content of total polyphenols and flavonoids.

Example 2. Antioxidant efficacy analysis

DPPH radical scavenging ability measurement

The extracts of Preparation Example 1 were each adjusted to a concentration of 100 μg/ml to measure antioxidant activity. The scavenging ability against DPPH radicals was determined by adding 100 µl of 0.2 mM 1,1-diphenyl-2-picryhydrazyl (DPPH) solution to 100 µl of each ethanol extract, reacting in the dark for 30 minutes, and measuring the absorbance at 490 nm. According to the DPPH radical scavenging ability was confirmed.

3 shows the DPPH radical scavenging ability measurement results, it can be seen that the cornus oil ( C. officinalis ) and purslane ( P. oleracea ) extracts show a high DPPH radical scavenging ability.

ABTS radical scavenging ability measurement

The extracts of Preparation Example 1 were each adjusted to a concentration of 100 μg/ml to measure antioxidant activity. After mixing 15 mL of 7 mM ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonate) solution and 264 μl of 140 mM potassium sulfate), the absorbance was measured at 630 nm by reacting for 30 minutes in a dark room.

Figure 4 shows the ABTS radical scavenging ability measurement results, safflower ( C. tinctorius ) shows the highest radical scavenging ability, cornus milk ( C. officinalis ) and purslane ( P. oleracea ) extracts also show a certain level of radical scavenging ability. I can.

Example 3. Tyrosinase (tyrosinase) activity inhibition experiment

The extracts of Preparation Example 1 were each adjusted to a concentration of 100 μg/ml to measure the inhibitory effect of tyrosinase activity. 50 µl of each extract or complex extract of two kinds of natural products, 50 µl of mushroom tyrosinase (700 U/ml), 100 µl of 0.1 sodium phosphate buffer (pH 6.8), and 100 µl of 2.5 mM L-DOPA were added to a 96-well plate and then 37 It was reacted at °C for 1 hour. Then, the absorbance was measured at 475 nm. The tyrosinase inhibition rate was calculated numerically using the following equation and shown in FIG. 5.

As can be seen in Figure 5, cornus ( C. officinalis ), safflower ( C. tinctorius ), purslane ( P. oleracea ), and white dandelion ( T. coreanum ) extracts are two types of complex extracts than when the single extract is In this case, it can be seen that a higher tyrosinase activity inhibitory effect is shown.

So far, the present invention has been looked at around its preferred embodiments. Those of ordinary skill in the art to which the present invention pertains will be able to understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (8)

A cosmetic composition for skin whitening comprising cornus milk and purslane extract as active ingredients. delete delete delete The method of claim 1,
The cornus milk or purslane extract is a cosmetic composition for skin whitening that is included in a concentration range of 1 to 200 μg/mL. The method of claim 1,
The composition is a cosmetic composition for skin whitening that inhibits tyrosinase. The method of claim 1,
The extract is a cosmetic composition for skin whitening that is extracted using water or an organic solvent The method of claim 7,
The organic solvent is a cosmetic composition for skin whitening selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane.

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