KR102334192B1 - Functional cosmetic composition comprising korean dandelion - Google Patents

Abstract

The present invention relates to a wrinkle-improving functional composition using a natural product. More specifically, the present invention provides a wrinkle-improving cosmetic composition including a Taraxacum albidum extract, an Oenothera extract and wild ginseng water, a composition for inner beauty, a pharmaceutical composition, and a health functional food composition. The composition is used as a cosmetic, pharmaceutical, and health functional food composition of natural ingredients for wrinkle improvement, including Taraxacum albidum and Oenothera, which are special crops in Gangwon-do with excellent antioxidant activity and elastase inhibitory activity, thereby reducing side effects such as toxicity of previously used chemicals and being used safely and effectively to improve skin wrinkles.

Description

Functional cosmetic composition containing white dandelion as an active ingredient {FUNCTIONAL COSMETIC COMPOSITION COMPRISING KOREAN DANDELION}

The present invention relates to a functional cosmetic composition comprising white dandelion as an active ingredient.

Recently, as interest in natural materials and eco-friendliness increases, consumers' preference for organic products and natural products is no longer limited to food and beverages, but is also attracting attention from cosmetic materials. The development of cosmetic materials using safe natural materials is being actively conducted to prevent concerns about human toxicity, and accordingly, the domestic and overseas natural cosmetics market is also growing every year.

The natural cosmetics market, which was worth $34.5 billion (about 38.7 trillion won) in 2018, is expected to grow at an average annual rate of 5.2%, reaching $54.5 billion in global sales by 2027. According to Future Market Insights (FMI), growing consumer concerns about the side effects of chemicals in cosmetics and toiletries, and the dissemination of knowledge about the benefits of natural ingredients and the rise of ethical consumerism It appears that growth is accelerating.

White dandelion (Korean dandelion, Taraxacum coreanum ) is a perennial plant belonging to the Compositae family. In oriental medicine, dried outflowers are collected before or after flowering in spring and summer and used for detoxification, anti-inflammatory and diuretic purposes. has been used In addition, there is a record that the young leaves were used as herbs and the roots were used for medicinal purposes. However, its use as a functional cosmetic using it is not known yet.

Republic of Korea Patent Registration No. 10-0878080 (2009.01.13. Announcement)

An object of the present invention is to provide a functional composition for wrinkle improvement using a natural product.

In order to achieve the above object, the present invention provides a wrinkle improvement cosmetic composition comprising a white dandelion extract, an evening primrose extract and wild ginseng water.

The present invention provides a composition for inner beauty comprising a white dandelion extract, an evening primrose extract and wild ginseng water.

The present invention provides a wrinkle-improving pharmaceutical composition comprising a white dandelion extract, an evening primrose extract and wild ginseng water.

In addition, the present invention provides a health functional food composition for improving wrinkles comprising a white dandelion extract, an evening primrose extract and wild ginseng water.

The composition according to the present invention contains white dandelion, evening primrose, etc., which are special crops of Gangwon-do having excellent antioxidant activity and elastase inhibitory activity, so that it can be used as a cosmetic, pharmaceutical, and health functional food composition of natural ingredients for wrinkle improvement. have.

The composition according to the present invention can be used for improving skin wrinkles more safely and effectively by reducing side effects such as toxicity of previously used chemicals by utilizing natural ingredients.

1 shows a special crop of Gangwon-do for the production of standard raw materials for functional cosmetics according to an embodiment of the present invention.
2 and 3 are a raw material extraction process according to an embodiment of the present invention, FIG. 2 is an organic solvent extraction process of white dandelion and evening primrose, and FIG. 3 is a schematic diagram of a reflux extraction process of wild ginseng.
Figure 4 shows the overall process of the cosmetic standard raw material production using the Gangwon-do specialty crop extract according to an embodiment of the present invention.
5 is a cytotoxicity test result of the cosmetic composition prepared according to the above example.
6 and 7 are an experiment confirming the wrinkle improvement function of the cosmetic composition prepared according to the above example, FIG. 6 is a DPPH radical scavenging activity test result, and FIG. 7 is an elastase inhibitory activity test result.

Hereinafter, the present invention will be described in detail.

The present invention provides a cosmetic composition for wrinkle improvement comprising a white dandelion extract, an evening primrose extract and wild ginseng water, which are special crops of Gangwon-do.

Preferably, the composition may include 5-15% by weight of white dandelion extract, 10-30% by weight of evening primrose extract, and the remaining amount of wild ginseng water.

Preferably, the composition may include white dandelion extract, evening primrose extract and wild ginseng water in a weight ratio of 1: (1 to 2): (1 to 8), more preferably 1:2:7 by weight.

As used herein, the term "extract" refers to a substance obtained by extracting a component of a natural product regardless of an extraction method, an extraction solvent, an extracted component, or the form of an extract. It may include any material obtainable by processing or processing by the method.

The extract may be extracted according to a method commonly used in the art, for example, hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc., which are performed alone or in combination of two or more methods. can be

Preferably, the white dandelion extract may be an extract obtained by extracting white dandelion ( Taaxacum coreanum Nakai) with an aqueous (C1-C4) alcohol solution, and more preferably, the flower, leaf, root part, or a mixture thereof of the white dandelion. may be extracted with an aqueous ethanol solution, but is not limited thereto.

Preferably, the evening primrose extract may be an extract obtained by extracting evening primrose ( Oenothera biennis ) with an aqueous (C1-C4) alcohol solution, and more preferably, evening primrose hay may be extracted with an aqueous ethanol solution, but is not limited thereto.

Preferably, the wild ginseng water may be a water vapor collected by reflux extraction of wild ginseng, but is not limited thereto.

As used herein, "wrinkle improvement" means suppressing or inhibiting the generation of wrinkles on the skin, or alleviating the already generated wrinkles. Wrinkle generation is mainly caused by a decrease in collagen or elastin, and the decrease in collagen or elastin may be caused by excessive oxidative stress or ultraviolet irradiation.

As used herein, the term "cosmetics" may include functional cosmetics having an effect of preventing or improving skin aging, wrinkle generation, etc., and the functional cosmetics are professional functional cosmetics emphasizing physiologically active efficacy and effects, unlike general cosmetics. As defined by the Cosmetics Act, it means a cosmetic that emphasizes specific efficacy and effects such as wrinkle improvement.

Since the composition has excellent antioxidant activity and elastase inhibitory activity, it can be used as a cosmetic composition for improving wrinkles.

The wrinkle improvement may include conventional skin protection effects such as skin wrinkle prevention, improvement, skin aging inhibition or improvement. In addition, since collagen and elastin are involved in the mechanical firmness of the skin, the bonding strength and elasticity of tissues, etc., effects such as skin elasticity improvement, skin regeneration, wound healing, and repair and regeneration of damaged skin tissue can be obtained by the composition.

The composition may further include one or more components selected from the group consisting of adenosine, hyaluronate, and glycerin, but is not limited thereto, and the cosmetic raw material list registered with the Ministry of Food and Drug Safety and ICID (International Cosmetic Ingredients Collection) ) may further include one or more of the listed ingredients.

For example, the cosmetic composition may include an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a fragrance, a surfactant, an emulsifier, a filler, a sequestering agent, a preservative, a vitamin, a blocking agent, It may further comprise one or more adjuvants commonly used in the cosmetic field, such as wetting agents, dyes and pigments, hydrophilic or lipophilic actives, or any other ingredients commonly used in cosmetics.

The vitamin may include a water-soluble or oil-soluble vitamin, and the water-soluble vitamin is a water-soluble vitamin that can be formulated in cosmetics, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, and nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like, and more preferably vitamin C, which helps collagen synthesis. In addition, salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) or derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) may be included, and the water-soluble vitamin is a microorganism transformation method , can be obtained by a conventional method such as a purification method from a culture of a microorganism, an enzymatic method or a chemical synthesis method.

The cosmetic composition according to the present invention may be prepared in any formulation conventionally prepared in the technical field to which the present invention pertains. For example, it may be formulated as a lotion, lotion, essence, cream, paste, gel, solution, suspension, emulsion, oil, wax, pack, powder, foundation, spray, surfactant-containing cleansing, etc., but is limited thereto. it is not going to be

More specifically, flexible lotion, nourishing lotion, astringent lotion, nourishing cream, massage cream, milk lotion, powder, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, powder foundation, emulsion foundation , wax foundation, cleansing cream, cleansing foam, cleansing water, soap, pack, stick product, balm type product, spray or powder formulation.

When the cosmetic composition according to the present invention is a cream or gel formulation, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be further included as a carrier component. can

When the cosmetic composition is a solution or emulsion formulation, a solvent, solvating agent or emulsifying agent, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid It may further include esters and the like.

When the cosmetic composition is a suspension formulation, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be further included.

When the cosmetic composition is in a powder or spray formulation, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be further included as a carrier component, and in particular, in the case of a spray formulation, additional chlorofluorohydrocarbon , may further include a propellant such as propane/butane or dimethyl ether.

When the cosmetic composition is a surfactant-containing cleansing formulation, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glycerides, fatty acid diethanolamide, vegetable oil, linoline derivatives or ethoxylated glycerol fatty acid esters may be further included.

The cosmetic composition according to the present invention may be used alone or by being applied in duplicate, or it may be used by applying it overlappingly with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be changed according to the skin condition or taste of the user.

The present invention provides a composition for inner beauty comprising a white dandelion extract, an evening primrose extract and wild ginseng water.

As used herein, "inner beauty" means eating cosmetics, and not only temporarily improving the skin surface by artificial methods such as applying cosmetics, but also providing healthy skin from the inside and the inside through food, etc. means caring for

Preferably, the composition may include 5-15% by weight of white dandelion extract, 10-30% by weight of evening primrose extract, and the remaining amount of wild ginseng water.

Preferably, the composition may have a skin wrinkle improvement effect.

Corresponding features may be substituted for the above-mentioned parts.

The composition for inner beauty according to the present invention is an emollient, antioxidant, suspending agent, foaming agent, surfactant, purified water, ionic or non-ionic emulsifier, filler, preservative, vitamin, blocker, wetting agent, dye, pigment, hydrophilic or hydrophilic It may further include any one or more adjuvants selected from oily active agents, but is not limited thereto, but is not limited thereto, but is not limited thereto, but is not limited thereto. It may further include any one of a substance, a personal care compound, an active ingredient, and a cosmetic adjuvant, or a combination thereof.

The composition may consist of any one or more formulations selected from films for oral mucosa, tablets, syrups, capsules, pills, granules, powders, and aerosols, but is not limited thereto.

The present invention provides a wrinkle-improving pharmaceutical composition comprising a white dandelion extract, an evening primrose extract and wild ginseng water.

Since the composition has excellent antioxidant activity and elastase inhibitory activity, it can be used as a pharmaceutical composition for skin wrinkle improvement.

Corresponding features may be substituted for the above-mentioned parts.

The pharmaceutical composition according to the present invention may be prepared according to a conventional method in the pharmaceutical field. The pharmaceutical composition may be combined with a suitable pharmaceutically acceptable carrier according to the formulation, and if necessary, excipients, diluents, dispersants, emulsifiers, buffers, stabilizers, binders, disintegrants, solvents, etc. may be prepared further comprising have. The appropriate carrier and the like do not inhibit the activity or properties of the extract according to the present invention, and may be selected differently depending on the dosage form and formulation.

The pharmaceutical composition according to the present invention can be applied in any dosage form, and more specifically, it can be used by formulating oral dosage forms, external preparations, suppositories, and parenteral dosage forms of sterile injection solutions according to conventional methods.

The solid dosage form among the oral dosage forms is in the form of tablets, pills, powders, granules, capsules, etc., and at least one or more excipients, for example, starch, calcium carbonate, sucrose, lactose, sorbitol, mannitol, cellulose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc may be included. In addition, the capsule formulation may further include a liquid carrier such as fatty oil in addition to the above-mentioned substances.

Among the oral dosage forms, liquid formulations include suspensions, solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients, for example, wetting agents, sweeteners, fragrances, preservatives, etc. may be included. have.

The parenteral formulation may include a sterile aqueous solution, a non-aqueous solution, a suspension, an emulsion, a freeze-dried formulation, and a suppository. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, Tween 61, cacao butter, laurin fat, glycerogelatin, and the like can be used. It is not limited thereto, and any suitable agent known in the art may be used.

In addition, the pharmaceutical composition according to the present invention may further add calcium, vitamin D3, or the like to enhance therapeutic efficacy.

The pharmaceutical composition according to the present invention may be administered in a pharmaceutically effective amount.

As used herein, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment and not to cause side effects.

The effective dose level of the pharmaceutical composition may be determined according to the purpose of use, the age, sex, weight and health status of the patient, the type of disease, the severity, the activity of the drug, the sensitivity to the drug, the administration method, the administration time, the administration route and the excretion rate, the treatment The duration, formulation, or concomitant use may be determined differently depending on factors including drugs and other factors well known in the medical field. For example, although not constant, generally 0.001 to 100 mg/kg, preferably 0.01 to 10 mg/kg, may be administered once to several times a day. The above dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition according to the present invention may be administered by an appropriate administration route according to the form of the formulation, and may be administered via various routes, either oral or parenteral, as long as it can reach the target tissue. The administration method does not need to be particularly limited, and for example, oral, rectal or intravenous, muscle, skin application, subcutaneous, respiratory inhalation, intrauterine dural or intracerebroventricular injection, etc. are administered in a conventional manner. can be

The pharmaceutical composition according to the present invention may be used alone for skin wrinkle improvement, or may be used in combination with surgery or other drug treatment.

The present invention provides a wrinkle-improving health functional food composition comprising a white dandelion extract, an evening primrose extract and wild ginseng water.

Since the composition has excellent antioxidant activity and elastase inhibitory activity, it can be used as a health functional food for skin wrinkle improvement.

Corresponding features may be substituted for the above-mentioned parts.

In the health functional food composition according to the present invention, the health functional food may be prepared in powder, granule, tablet, capsule, syrup or beverage, etc., and there is no limitation in the form that the health functional food can take, It can include any food with meaning. For example, beverages and various drinks, fruits and their processed foods (canned fruit, jam, etc.), fish, meat and their processed foods (ham, bacon, etc.), breads and noodles, cookies and snacks, dairy products (butter, cheese, etc.) ), etc. are possible, and may include all functional foods in a conventional sense. It may also include food used as feed for animals.

The health functional food composition according to the present invention may be prepared by further including pharmaceutically acceptable food additives and other suitable auxiliary ingredients commonly used in the art. For example, flavoring agents, natural carbohydrates, sweeteners, vitamins, electrolytes, coloring agents, pectic acid, alginic acid, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents, etc. can

In particular, as the natural carbohydrate, monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol can be used. , As the sweetener, natural sweeteners such as taumatine and stevia extract, or synthetic sweeteners such as saccharin and aspartame can be used.

The effective dose of the extract contained in the health functional food according to the present invention may be appropriately adjusted according to the purpose of skin wrinkle improvement. The composition has the advantage of not having side effects that may occur during long-term use of general drugs by using natural products and food as raw materials, and has excellent portability, and can be taken as an adjuvant for skin wrinkle improvement.

In addition, the present invention may provide a reagent composition for inhibiting elastase activity comprising a white dandelion extract, an evening primrose extract and wild ginseng water.

Corresponding features may be substituted for the above-mentioned parts.

Hereinafter, examples will be described in detail to help the understanding of the present invention. However, the following examples are merely illustrative of the content of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

<Example 1> Preparation of functional cosmetic composite raw materials using Gangwon-do specialty crops

1. Raw material

Poyeonggong (Yanggu white dandelion, hay 600g), evening primrose (600g hay), and Pyeongchang wild ginseng water (3 kg of extract by steam extraction) were prepared (FIG. 1).

2. Pretreatment

The prepared raw fruits and dried raw materials were pre-treated in the following order and used as extraction raw materials for ease of use and unification of raw material supply and demand extraction methods.

1) Low-temperature drying: Primary drying was performed for 12-15 hours while removing moisture inside the dryer at a low-temperature drying temperature of 30°C. After completion of the primary drying, the conservative moisture content of the sample is within 20%.

2) Freeze-drying: In order to prevent spoilage of the sample, ease of storage, and reduce the maximum moisture content, freeze-drying at -20°C to keep the minimum moisture content of 7% and the moisture content of the test sample at 13% or less to pre-treat the sample. did

3) Grinding: The powder pulverized to 40 mesh or less using a grinder (IKA A11 basic) was stored at -20°C and used in the experiment.

3. Extraction process

1) Poyeonggong (white dandelion)

Extraction experiments were conducted using samples obtained by mixing and pulverizing dried flowers, leaves, and roots of white dandelion. Hot water extraction was performed by adding 10 times distilled water to 100 g of the pulverized sample, followed by extraction three times for 2 hours at 100 ° C., and organic solvent extraction was performed using 10 times 70% ethanol in 100 g of the pulverized sample at 80 ° C. Extraction was performed three times for 3 hours.

Table 1 below compares the yield of white dandelion extract according to the two extraction methods.

Sample Average Yields
(%, dry basis) Test 1 Test 2 Test 3 hot water extraction 40.26 38.88 41.23 40.68 Organic solvent extraction method 51.17 51.20 53.28 49.04

Through this, it was found that the extraction yield of white dandelion was high in the organic solvent extraction method, and the optimal extraction method of white dandelion was set as the organic solvent extraction method, and the extraction process was standardized (FIG. 2).

2) Evening primrose

For extracts of evening primrose, seeds and stamens, the organic solvent extraction method is expected to be suitable through the study results of fat-soluble extracts. For hot water extraction, 14 times distilled water was added to 100 g of pulverized evening primrose hay sample, boiled at 100° C. for 2 hours, cooled at room temperature for 10 hours, and then extracted under reflux twice. For organic solvent extraction, 10 times 70% ethanol was added to 100 g of the pulverized sample, and then stirred extraction was performed at room temperature for 12 hours at 150 rpm using a stirrer at 25 ± 2 ° C. .

Table 2 below compares the yield of the evening primrose extract according to the two extraction methods.

Sample Average Yields
(%, dry basis) Test 1 Test 2 Test 3 hot water extraction 40.96 41.56 40.08 41.24 Organic solvent extraction (SE) 40.86 40.88 41.12 40.58

Referring to this, although the extraction yield of hot water extraction is high, the extraction yield of the organic solvent room temperature stirring extraction (SE) method is effective compared to the time of the extraction process, so it was standardized to the organic solvent extraction method (FIG. 2).

3) Goat ginseng water

Due to the high price of raw materials, we received WGDE directly produced by a wild ginseng grower and went through a concentration step to use it as a sample.

The extraction method notified by the manufacturer is as follows.

For extraction of wild ginseng water (WGDE), a distillation extractor was used. 1 L of distilled water was added to 100 g of dried wild ginseng, and hot water was performed by reflux extraction for 3 hours. After boiling, the water vapor evaporated after stopping the supply of cooling water to the reflux extractor was collected and used as a liquid (FIG. 3).

4. Centrifugation and functional ingredient concentration process

The concentration process was repeated as a method to remove the residues and organic solvents of the extract extracted by the above extraction process and to increase the component concentration.

1) Primary purification: filtration

Each extract was prepared according to Whatman No. 1 After the primary filtration process of filtering impurities, etc. with a filter paper, a filtration layer is formed using powdered activated carbon, and the extract is passed through the secondary filtration process to adsorb contaminants in the extract and separate solvents and chlorophyll components in plants was performed.

2) Secondary purification: centrifugation

The first purified extract was centrifuged at 2,000-3,000 rpm for 7-15 minutes using a centrifugal device Labogene 416G, and only the supernatant was filtered to fractionate the crude extract step by step. Plant active ingredients were extracted and purified.

3) Concentration: reflux concentration (rotary reduced pressure concentration, vacuum evaporation method)

This is a step to obtain high-purity components by adding a concentration step to the components of the extract that has completed the second purification step. The pure extract of the extract is dried by evaporating a solvent such as ethanol of the extract with a reduced pressure concentrator EYELA N-1300 and drying and waste treatment. was aggregated and fractionated using layer separation. The temperature of the water bath was maintained at 35 ° C. to 42 ° C., the vacuum for the boiling point was 175 mbar, and the rotation speed was 140 to 200 rpm (solvent: ethanol-140 rpm, distilled water-200 rpm).

5. Mixing Mixing and Formulation Separation Process

Cosmetic ingredients were developed through mixing, mixing, and freeze-drying processes by functionalities of the high-purity extracts that went through the above-mentioned concentration process. After freeze-drying the concentrated extract, it was stored at -40°C. Mixed powder complex can be divided into a method of mixing powdered raw materials by freeze-drying each extract and a method of freeze-drying and powdering a liquid mixed complex raw material. The ratio of residues was high, so the former method was used to prepare raw materials in powder form. The final standardization process is shown in FIG. 4 .

Using the Waters HPLC system, the functional main components of the extracts of purpurea (white dandelion), evening primrose and wild ginseng were confirmed as follows.

Table 3 below shows the main functional components of each extract using HPLC analysis by applying C18-Column.

<Preparation Example 1> Manufacturing of functional cosmetics - GSP_A (Gangwon Special Plant_Anti-aging)

Functional cosmetic GSP_W was prepared with the ingredients and contents of Table 4 below, and its functionality was confirmed.

<Experimental Example 1> Cytotoxicity test

In order to evaluate the safety as a cosmetic material, the cell viability was measured by MTT assay. B16 F10 mouse melanoma cells (Korea Cell ^{Line Bank) were aliquoted at 1×10 4} cells/ml and cultured for 24 hours, followed by a fresh medium containing a sample of the extract diluted to a concentration of 10 to 1,000 μg/ml. was replaced with and incubated for 24 hours again. After adding 20 μl of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide, Sigma) to each well, 37℃, 5% CO ₂ incubator (Sanyo, Japan) ), the formazan formed after 2 hours of incubation was dissolved in 200 μl DMSO, and absorbance was measured at 595 nm with an ELISA reader (PowerWaveXS2, BioTek, USA).

Cell viability was calculated by Equation 1 below.

<Equation 1> Cell viability(%)=[(Exp.-Blank)/Control]×100

Using the cosmetic GSP_A and vitamin C prepared according to Preparation Example 1 as a control, the extract was diluted to a concentration of 10-1,000 μg/ml and tested for cytotoxicity, as shown in FIG. 5 , GSP_A was 1,000 μg/ml showed that it was safe even at high concentrations by showing more than 80% cell viability in

Experimental results were expressed as mean ± standard deviation (Mean ± S.D.), and statistical processing was analyzed using Student'sunpairedt-test and ANOVA, and it was determined that there was a significant difference when p <0.05. The same was applied to the following experiments.

<Experimental Example 2> Confirmation of optimal mixing ratio

In order to confirm each optimal mixing ratio, an experiment on the moisturizing effect of the mixed extract was conducted. The moisturizing rule is the most basic measure for improving the skin barrier as a basic characteristic of cosmetics.

Table 5 below shows the moisturizing effect over time according to each extract mixing ratio. Referring to this, when the mixing ratio of the wrinkle-improving functional extract (GSP_A) per 100 g of the total weight as a weight ratio of the liquid complex cosmetic is 1:2:7, moisturizing It was confirmed that the effect was the best. The raw material was liquefied (dissolved in 100 times distilled water, and the supernatant was used after 30 minutes) in the aqueous phase, goat ginseng water, and the raw material was heated and stirred at 75° C. and 11,000 rpm for 15 minutes.

<Experimental Example 3> Wrinkle improvement functional confirmation

White dandelion, evening primrose, and wild ginseng extracts were mixed (ratio of 1:2:7) to confirm the wrinkle improvement function.

1. Confirmation of antioxidant activity

Antioxidant activity by the DPPH method was measured according to the Blois method. After adding 3.8 mL of 0.4 mM DPPH to 0.2 mL of the GSP_A extract, mixing for 10 seconds, reacting at room temperature for 30 minutes, and measuring absorbance at 517 nm wavelength. The negative control group was tested by adding the same amount of ethanol instead of the sample, and as a positive control group, synthetic antioxidant BHA (butylated hydroxyanisole) was used in the same way.

As a result, as shown in FIG. 6 , the DPPH radical scavenging ability of the GSP_A extract was increased in a concentration-dependent manner. At a concentration of 500 μg/mL, an effect similar to that of BHA was exhibited, and at the remaining concentrations (10, 50, 100 μg/mL), the effect was lower than that of BHA.

Each component of the mixed raw material has antioxidant compounds, and in particular, it has been previously confirmed through HPLC analysis that they contain flavonoid compounds. Through this, it can be determined that the GSP_A extract according to the present invention has high antioxidant activity.

2. Confirmation of inhibition of elastase activity

To 200 μl of substrate 1.0 mM　N-succinyl-(Ala)3-p-nitroanilide (Sigma), 20 μl of a sample solution diluted with 100-1,000 μg/ml of GSP_A Complex and 10 μl of 2.5U elastase (porcinepancreas solution) were added at 25°C. After reacting for 10 minutes, absorbance was measured at 410 nm with an ELISA reader. As a control group, adenosine, which is widely used as a functional cosmetic material for wrinkle improvement, was used.

As a result, as shown in FIG. 7 , at a concentration of 500 μg/ml of the GSP_A Complex raw material, an inhibitory rate of 68%, a wrinkle improvement effect similar to that of adenosine, a control group, was exhibited. Referring to the literature, the plants showing the elastase inhibitory effect at a concentration of 500 μg/ml were cornus controversa Hemsl. 80%, Machilus japonica Siebold 77%, cedar ( Cryptomeria japonica ) 73% and Wild rose ( Rosa multiflora ) showed an inhibitory effect of 73%. Compared to the above plants, the elastase inhibition rate of the GSP_A Complex raw material can be judged to show a similar or relatively superior effect.

<Experimental Example 4> Raw material test

Raw material tests were performed on properties, odor, stability (48 hrs), pH, lead (Pb) content, arsenic (As) content, and microorganisms. Among the test items, the Pb (lead) content test is a test method applied to clay or soil-related samples. In this experimental example, 7 kinds of experiments were performed by replacing the viscosity test for liquid raw materials for each function. In this case, since it cannot have viscosity, six types of tests were conducted in consultation with the client.

Table 6 below is the raw material test result obtained by collecting 100 g of GSP_A liquid raw material,

Table 7 below shows the raw material test results obtained by collecting 100 g of GSP_A powder raw material.

As described above in detail a specific part of the content of the present invention, for those of ordinary skill in the art, it is clear that this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby. do. That is, the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (14)

It contains white dandelion extract, evening primrose extract and wild ginseng water in a weight ratio of 1: (1-2): (7-8), wherein the white dandelion extract is an extract obtained by extracting white dandelion (Taraxacum coreanum Nakai) with an aqueous ethanol solution, and Evening primrose extract is an extract extracted with an aqueous ethanol solution and then stirred at room temperature, and the wild ginseng water is a wrinkle improvement cosmetic composition, characterized in that it is a water vapor collected by reflux extraction of wild ginseng. delete delete delete delete The method of claim 1,
The composition is
A cosmetic composition for improving wrinkles, characterized in that it further comprises one or more components selected from the group consisting of adenosine, hyaluronate, and glycerin. The method of claim 1,
The composition is
having a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, essences, lotions, powders, soaps, surfactant-containing cleansings, oils, powder foundations, emulsion foundations, wax foundations and sprays A cosmetic composition for wrinkle improvement, characterized in that. It contains white dandelion extract, evening primrose extract and wild ginseng water in a weight ratio of 1: (1-2): (7-8), wherein the white dandelion extract is an extract obtained by extracting white dandelion (Taraxacum coreanum Nakai) with an aqueous ethanol solution, and Evening primrose extract is an extract extracted with an aqueous ethanol solution and then stirred at room temperature, and the wild ginseng water is a water vapor collected by reflux extraction of wild ginseng. A composition for inner beauty having a skin wrinkle improvement effect. delete delete 9. The method of claim 8,
The composition is
Any one or more adjuvants selected from emollients, antioxidants, suspending agents, foaming agents, surfactants, purified water, ionic or non-ionic emulsifiers, fillers, preservatives, vitamins, blocking agents, wetting agents, dyes, pigments, hydrophilic or lipophilic active agents Composition for inner beauty (inner beauty), characterized in that it further comprises. 9. The method of claim 8,
The composition is
A composition for inner beauty, characterized in that it consists of any one or more formulations selected from oral mucosa films, tablets, syrups, capsules, pills, granules, powders, and aerosols. delete It contains white dandelion extract, evening primrose extract and wild ginseng water in a weight ratio of 1: (1-2): (7-8), wherein the white dandelion extract is an extract obtained by extracting white dandelion (Taraxacum coreanum Nakai) with an aqueous ethanol solution, and Evening primrose extract is an extract extracted with an aqueous ethanol solution and then stirred at room temperature, and the wild ginseng water is a wrinkle improvement health functional food composition, characterized in that it is a water vapor collected by reflux extraction of wild ginseng.

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