KR102315700B1 - Composition for treating or preventing acne comprising elaeagnus umbellata leaves extract - Google Patents

Abstract

The present invention relates to a composition for treating or preventing acne comprising a Bodhi tree leaf extract, and more particularly, to a cosmetic composition for improving or preventing acne, a health food composition or a pharmaceutical composition comprising the Bodhi tree leaf extract as an active ingredient . In addition, it provides an antibacterial composition for inhibiting the activity of Propionibacterium acnes ( Propionibacterium acnes , P. acnes ) containing an extract of Bodhi tree leaf as an active ingredient.
The Bodhi tree leaf extract is extracted under optimal extraction conditions and has excellent antibacterial activity against the acne bacterium Propionibacterium acnes , so it can effectively treat, improve or prevent acne.

Description

A composition for treating or preventing acne comprising an extract of Bodhi tree leaves {COMPOSITION FOR TREATING OR PREVENTING ACNE COMPRISING ELAEAGNUS UMBELLATA LEAVES EXTRACT}

The present invention relates to a composition for treating or preventing acne comprising an extract of Bodhi tree leaf.

Acne is an inflammatory skin disease that mainly causes comedones, papules, pus blisters, nodules, and pseudocysts on the face, neck, chest, back and shoulders. Acne usually occurs in the early teens, but symptoms may worsen around the age of 20, and may also occur in adults in their 30s and 40s. It usually disappears after several years without treatment, but if left untreated, it can leave a permanent scar, which is a cosmetic problem. Therefore, it is important to take care of the skin to prevent acne from occurring on a regular basis and to pay attention to the treatment of acne.

The exact cause of acne is still unknown, but it is known that several causes act in a complex way. The male hormone increased during puberty enlarges the sebaceous glands (skin oil glands) of the skin, and an oily substance called sebum is produced in these sebaceous glands. It causes them to fall out quickly, and these cells aggregate and block the pores of the hair follicles to become a comedone, the basic lesion of acne.

In addition, the lipolytic enzyme secreted by the bacteria resident in the hair follicle decomposes the triglycerides in the sebum to form free fatty acids. It is released to cause erythema, edema, and pus.

Linden trees (Elaeagnus umbellata Thunb. Autumn olive) are the deciduous broad-leaved shrub belonging to elaeagnaceae (Elaeagnaceae) lime hawthorn (Elaeagnus), the country widely distributed in hapcheon county gayamyeon known as barley shit trees ppeolttong, pingkeupap lime in the private .

Analysis of general components and lycopene content of Bodhi tree fruits, leaves, flowers, and fruits, antioxidant and cancer cell proliferation inhibitory effects of fruit extracts, and comparison of antioxidant activity of fruit and leaf solvent fractions have been reported. There are reports of component analysis, antioxidant and anticancer, whitening effect and antiplatelet effect of fruit extract, and leaves, roots and branches of Bodhi tree are also used to treat cough and asthma.

However, there are not many systematic efficacy studies of Bodhi tree, and although it is approved for food, there is little use as a food material.

Since constant management is required to treat acne, there is a need for natural materials that are safe and effective in treating acne with fewer side effects even if they are taken or contacted for a long time.

Republic of Korea Patent Publication No. 10-2009-0029503 (published on March 23, 2009)

It is an object of the present invention to provide a composition for treating or preventing acne using a natural extract having excellent antibacterial activity.

Another object of the present invention is to provide an antibacterial composition using the natural extract.

The cosmetic composition for improving or preventing acne according to the present invention may contain a Bodhi tree leaf extract as an active ingredient.

The health food composition for improving or preventing acne according to the present invention may contain a Bodhi tree leaf extract as an active ingredient.

The pharmaceutical composition for treating or preventing acne according to the present invention may contain a Bodhi tree leaf extract as an active ingredient.

The antibacterial composition for inhibiting Propionibacterium acnes , P. acnes activity according to the present invention may contain a Bodhi tree leaf extract as an active ingredient.

The Bodhi tree leaf extract according to the present invention can be used as an effective antibacterial composition to exhibit excellent antibacterial activity against the acne bacterium Propionibacterium acnes by being extracted under optimal extraction conditions.

In addition, the Bodhi tree leaf extract can be used as a cosmetic composition, health food composition, or pharmaceutical composition for improving or preventing acne, and by using a natural product such as the Bodhi tree leaf extract, acne can be effectively treated or prevented without side effects.

Figure 1 shows the antibacterial effect on the Propionibacterium acnes (Propionibacterium acnes, P. acnes ) strain for each extraction condition of a Bodhi tree leaf extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The present inventors have completed the present invention by confirming the optimal extraction conditions of the Bodhi tree leaf extract and confirming the antibacterial activity against its acne bacteria, Propionibacterium acnes , P. acnes.

The present invention provides a cosmetic composition for improving or preventing acne comprising a bodhi tree leaf extract as an active ingredient.

As used herein, the term "extract" refers to a material obtained by extracting a component of a natural product regardless of an extraction method, an extraction solvent, an extracted component, or the form of an extract, and the material obtained by extracting the component of a natural product is extracted It may include any material obtainable by processing or processing by the method.

As used herein, "cosmetics" may include functional cosmetics having an effect such as alleviating or preventing acne-prone skin. The functional cosmetic means a cosmetic with professional functionality that emphasizes physiologically active efficacy and effect, unlike general cosmetics.

In the cosmetic composition according to the present invention, the bodhi tree leaf extract may be extracted using a C1 to C4 alcohol or an aqueous solution thereof as a solvent, and preferably 60 to 80% by weight of an aqueous ethanol solution as a solvent. can

Preferably, the Bodhi tree leaf extract is immersed in 60 to 80 wt%, more preferably 70 wt% ethanol aqueous solution at 70 to 90°C, more preferably 80°C for 5 to 7 hours, more preferably It may be extracted for 6 hours.

The bodhi tree leaves may be collected or grown in nature, or purchased commercially.

The bodhi tree leaf extract may be extracted according to a method commonly used in the art, for example, hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method, etc., which are performed alone or by using two or more methods It can be performed in combination.

In the cosmetic composition according to the present invention, the Bodhi tree leaf extract may have antibacterial activity against the acne bacteria Propionibacterium acnes. According to an embodiment of the present invention , the antibacterial activity against Propionibacterium acnes ( P. acnes ) in the Bodhi tree leaf extract was confirmed to be superior to that of chloramphenicol, a positive control.

In the cosmetic composition according to the present invention, the bodhi tree leaf extract may be included in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total cosmetic, but is not limited thereto.

The cosmetic composition according to the present invention may further include one or more of the ingredients listed in the list of cosmetic raw materials registered with the Food and Drug Administration and ICID (International Cosmetic Ingredients). For example, the cosmetic composition may include an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a fragrance, a surfactant, an emulsifier, a filler, a sequestering agent, a preservative, a vitamin, a blocking agent, It may further comprise one or more adjuvants commonly used in the cosmetic field, such as wetting agents, dyes and pigments, hydrophilic or lipophilic actives, or any other ingredients commonly used in cosmetics.

The cosmetic composition according to the present invention may be prepared in any formulation conventionally prepared in the technical field to which the present invention pertains. For example, it may be formulated as softening lotion, nutrient lotion, emulsion, lotion, cream, paste, gel, solution, suspension, oil, wax, pack, powder, foundation, spray, surfactant-containing cleansing, etc. It is not limited. More specifically, skin lotion, skin softener, skin toner, nourishing cream, massage cream, milk lotion, powder, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, powder foundation, emulsion foundation , cleansing cream, cleansing foam, cleansing water, soap, pack, stick product, balm type product, spray or powder formulation.

Preferably, the cosmetic composition may be a surfactant-containing cleansing formulation, and in this case, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate , sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester and the like may be further included.

When the cosmetic composition according to the present invention is a cream or gel formulation, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be further included as a carrier component. can

When the cosmetic composition is a solution or emulsion formulation, a solvent, a solvating agent or an emulsifying agent, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid It may further include esters and the like.

When the cosmetic composition is a suspension formulation, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be further included.

When the cosmetic composition is in a powder or spray formulation, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be further included as a carrier component, and in particular, in the case of a spray formulation, additional chlorofluorohydrocarbon , may further include a propellant such as propane/butane or dimethyl ether.

The cosmetic composition according to the present invention may be used alone or by being applied in duplicate, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.

The present invention provides a health food composition for improving or preventing acne comprising a bodhi tree leaf extract as an active ingredient.

The bodhi tree leaf extract has antibacterial activity against the acne bacterium Propionibacterium acnes and can be used as a health food composition for improving or preventing acne. Corresponding features may be substituted for the above-mentioned parts.

In the health food composition according to the present invention, the health food may be prepared in powder, granule, tablet, capsule, syrup or beverage, etc. for the purpose of improving or preventing acne, and there is no limitation in the form that the food can take, It may include all foods in the ordinary sense. For example, beverages and various drinks, fruits and their processed foods (canned fruit, jam, etc.), fish, meat and their processed foods (ham, bacon, etc.), breads and noodles, cookies and snacks, dairy products (butter, cheese, etc.) ), etc. are possible, and may include all functional foods in a conventional sense. It may also include food used as feed for animals.

The health food composition according to the present invention may be prepared by further comprising a food pharmaceutically acceptable food additive (food additive) and other suitable auxiliary ingredients commonly used in the art.

Unless otherwise specified, the suitability as a food additive can be judged according to the standards and standards for the relevant item in accordance with the general rules and general test methods of the Food Additives Code approved by the Food and Drug Administration. The items listed in the 'Food Additives Codex' include, for example, chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; natural additives such as persimmon pigment, licorice extract, crystalline cellulose, high pigment, and guar gum; Mixed preparations, such as a sodium L-glutamate preparation, a noodle-added alkali agent, a preservative preparation, and a tar dye preparation, etc. are mentioned.

The other auxiliary ingredients include, for example, flavoring agents, natural carbohydrates, sweeteners, vitamins, electrolytes, colorants, pectic acid, alginic acid, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents, etc. may further contain. In particular, as the natural carbohydrate, monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol can be used. , as the sweetener, natural sweeteners such as taumatine and stevia extract or synthetic sweeteners such as saccharin and aspartame may be used.

The effective dose of the Bodhi tree leaf extract contained in the health food according to the present invention may be appropriately adjusted according to the purpose of use, such as improvement or prevention of acne. The composition has the advantage of not having side effects that may occur during long-term use of general drugs by using natural products and food as raw materials, and has excellent portability, and can be taken as an adjuvant for improving or preventing acne.

The present invention provides a pharmaceutical composition for the treatment or prevention of acne containing a bodhi tree leaf extract as an active ingredient.

The bodhi tree leaf extract has antibacterial activity against the acne bacterium Propionibacterium acnes and can be used as a pharmaceutical composition for treating or preventing acne. Corresponding features may be substituted for the above-mentioned parts.

The pharmaceutical composition according to the present invention may be prepared according to a conventional method in the pharmaceutical field. The pharmaceutical composition may be combined with a suitable pharmaceutically acceptable carrier according to the formulation, and if necessary, excipients, diluents, dispersants, emulsifiers, buffers, stabilizers, binders, disintegrants, solvents, etc. may be prepared further comprising have. The appropriate carrier and the like do not inhibit the activity and properties of the Bodhi tree leaf extract according to the present invention, and may be selected differently depending on the dosage form and formulation.

The pharmaceutical composition according to the present invention can be applied in any dosage form, and more specifically, it can be used by formulating oral dosage forms, external preparations, suppositories, and parenteral dosage forms of sterile injection solutions according to conventional methods.

The solid dosage form among the oral dosage forms is in the form of tablets, pills, powders, granules, capsules, etc., and at least one or more excipients, for example, starch, calcium carbonate, sucrose, lactose, sorbitol, mannitol, cellulose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc may be included. In addition, the capsule formulation may further include a liquid carrier such as fatty oil in addition to the above-mentioned substances.

Among the oral dosage forms, liquid formulations include suspensions, solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients, for example, wetting agents, sweeteners, fragrances, preservatives, etc. may be included. have.

The parenteral formulation may include a sterile aqueous solution, a non-aqueous solution, a suspension, an emulsion, a freeze-dried formulation, and a suppository. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, Tween 61, cacao butter, laurin fat, glycerogelatin, and the like can be used. Without being limited thereto, any suitable agent known in the art may be used.

In addition, the pharmaceutical composition according to the present invention may further add _{calcium or vitamin D 3 or the like to enhance therapeutic efficacy.}

The pharmaceutical composition according to the present invention may be administered in a pharmaceutically effective amount.

As used herein, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment and not to cause side effects.

The effective dose level of the pharmaceutical composition is determined by the purpose of use, the age, sex, weight and health status of the patient, the type of disease, severity, drug activity, sensitivity to drug, administration method, administration time, administration route and excretion rate, treatment It may be determined differently depending on factors including the duration, formulation or concurrent use of drugs and other factors well known in the medical field. For example, although not constant, generally 0.001 to 100 mg/kg, preferably 0.01 to 10 mg/kg, may be administered once to several times a day. The above dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition according to the present invention may be administered by an appropriate administration route according to the form of the formulation, and may be administered through various routes, either oral or parenteral, as long as it can reach the target tissue. The administration method does not need to be particularly limited, and for example, oral, rectal or intravenous, muscle, skin application, subcutaneous, respiratory inhalation, intrauterine dural or intracerebroventricular injection, etc. are administered in a conventional manner. can be

The pharmaceutical composition according to the present invention may be used alone for the treatment or prevention of acne, or may be used in combination with surgery or other drug treatment.

In addition, the present invention provides an antibacterial composition for inhibiting the activity of Propionibacterium acnes , P. acnes , which is an acne bacterium, containing an extract of Bodhi tree leaf as an active ingredient.

As used herein, the term “antibacterial” refers to a property that resists microorganisms such as bacteria or fungi, and inhibits the growth or proliferation of the microorganisms. Preferably, in the present invention, it is possible to inhibit the activity of Propionibacterium acnes ( P. acnes ). Corresponding features may be substituted for the above-mentioned parts.

Hereinafter, to help the understanding of the present invention, examples will be described in detail. However, the following examples are merely illustrative of the contents of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

<Example 1> Preparation of extracts from Bodhi tree leaves

By crushing dried bodhi tree leaves, ethanol solvent conditions (0%, 20%, 50% 70%), temperature conditions (25℃, 60℃, 80℃), time conditions (3, 6, 12, 24, 48) time) were extracted. The extracted sample was filtered with Advantec (No. 5A, Tokyo, Japan) filter paper, concentrated using a vacuum concentrator, and then freeze-dried and used as a sample for analysis.

Table 1 shows the extraction yield for each extraction condition of the Bodhi tree leaf extract prepared according to an embodiment of the present invention. The extraction yield of the Bodhi tree leaf extract was calculated as the relative ratio of the dry weight of the Bodhi tree leaf and the extract weight.

Referring to Table 1, it was found that the extraction yield of the Bodhi tree leaf extract was the best when the Bodhi tree leaf was immersed in 70% ethanol and extracted at 80° C. for 6 hours.

<Example 2> Confirmation of antibacterial effect on acne bacteria of Bodhi tree leaf extract

The antibacterial effect on the bodhi tree leaf extract was confirmed using Propionibacterium acnes , P. acnes , Gram-positive bacteria.

The antibacterial activity was measured by measuring the clear zone size of the Bodhi tree leaf extract by the disk diffusion method. The clear zone of the disk was checked after 24 hours. Propionibacterium acnes ( P. acnes ) is a skin flora, known as a strain that causes skin troubles, particularly acne. Chloramphenicol was used as a positive control.

* P. acnes strain culture conditions

- Number of bacteria: 2×10 ⁶ cfu/mL

- Medium: RCM medium

- Culture conditions: 37℃, 10% CO ₂ , stationary culture

1 shows the antibacterial effect on P. acnes strains according to the extraction conditions of a Bodhi tree leaf extract according to an embodiment of the present invention.

Referring to FIG. 1 , the clear zone of the extract obtained by immersing bodhi tree leaves in 70% ethanol at 80° C. for 6 hours showed the best antibacterial effect with a clear zone of 7.0 ± 0.4 mm. A positive control, chloramphenicol (chloramphenicol, 0.1 mg/mL), formed a 5.0 mm clear zone.

As described above in detail a specific part of the content of the present invention, for those of ordinary skill in the art, it is clear that this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby. do. That is, the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (8)

Contains bodhi tree leaf extract as an active ingredient,
A cosmetic composition for improving or preventing acne, characterized in that it has antibacterial activity against the acne bacteria, Propionibacterium acnes. The method of claim 1,
The bodhi tree leaf extract,
A cosmetic composition for improving or preventing acne, characterized in that it is immersed in 60 to 80% by weight of an aqueous ethanol solution and extracted at 70 to 90° C. for 5 to 7 hours. delete The method of claim 1,
The bodhi tree leaf extract,
A cosmetic composition for improving or preventing acne, characterized in that it is included in an amount of 0.001 to 50 parts by weight based on 100 parts by weight of the total cosmetic. The method of claim 1,
The composition is
A cosmetic composition for improving or preventing acne, characterized in that it has the formulation of softening lotion, nourishing lotion, nourishing cream, essence, pack or soap. Contains bodhi tree leaf extract as an active ingredient,
A health food composition for improving or preventing acne, characterized in that it has antibacterial activity against acne bacteria, Propionibacterium acnes. Contains bodhi tree leaf extract as an active ingredient,
Acne bacteria Propionibacterium acnes ( Propionibacterium acnes ) A pharmaceutical composition for treating or preventing acne, characterized in that it has antibacterial activity. An antibacterial composition for inhibiting the activity of Propionibacterium acnes ( Propionibacterium acnes , P. acnes ) containing a Bodhi tree leaf extract as an active ingredient.

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