KR102475898B1 - Cosmetic composition comprising Graviola antibiotic extract and method of preparing the same - Google Patents

Abstract

The present invention provides a cosmetic composition for promoting skin vitality containing a graviola antibacterial extract that promotes skin vitality such as skin improvement, skin soothing and skin moisturizing, and a manufacturing method thereof, thereby providing antioxidant, antibacterial activity, skin barrier activity, whitening effect and It has excellent anti-inflammatory activity, so it is effective in manufacturing essence for improving skin vitality, such as skin improvement, skin soothing and skin moisturizing.

Description

COSMETIC COMPOSITION COMPRISING Graviola antibiotic extract and method of preparing the same}

The present invention relates to a skin cosmetic composition comprising Graviola extract as an active ingredient, and more particularly, to a cosmetic composition for enhancing skin vitality containing a Graviola antibacterial extract that promotes skin vitality such as skin improvement, skin soothing and skin moisturizing and a manufacturing method thereof.

As human skin ages, the secretion of various hormones that internally regulate metabolism decreases, and the function of immune cells and the activity of cells decrease, resulting in a decrease in the biosynthesis of immune proteins and biocomponent proteins necessary for the body. Intrinsic aging or chronologic aging caused externally by various contaminants and ultraviolet rays causes the skin to become thinner and its elasticity to decrease, and as it is exposed to ultraviolet rays, keratinocytes and keratinocytes in the epidermis Melanocytes are damaged by UVB (Ultra Violet B) and are affected by extrinsic aging or photo aging.

In particular, in modern society, due to hormonal abnormalities caused by excessive stress and sudden changes in the external environment, the vitality of the skin deteriorates more quickly due to the influence of air pollutants that exceed the skin's defense mechanism or stronger ultraviolet rays.

In chronological aging, characteristic clinical skin findings include fine wrinkles, atrophy of the dermis, and a decrease in the subcutaneous fat layer, while in the photoaging process, rough and deep wrinkles appear and abnormal elastotic materials accumulate, making the skin look like leather. thick and loose

In the cells constituting the skin, cell activity decreases due to endogenous aging or photoaging, and the signal transduction system becomes incomplete, increasing the biosynthesis of MMPs (matrix metalloproteinases), which are enzymes that decompose skin tissue, and reducing the biosynthesis of collagen, resulting in wrinkles. It is known that this occurs, elasticity decreases, and melanin production, which causes skin darkening, increases. Therefore, substances that can thicken the skin by proliferating skin cells or increasing the matrix substances constituting the skin, substances that can inhibit the biosynthesis of MMPs that degrade collagen, the matrix substances constituting the skin, and inhibition of melanin biosynthesis Substances that can do this have been found to alleviate skin symptoms such as wrinkles, loss of elasticity, and darkening of the skin.

Recently, according to the environment-friendly and nature-oriented trend, active ingredients used in cosmetics are not only chemical substances but also plant-derived natural products based on their usefulness, and natural ingredients with fewer side effects on the skin are mixed in cosmetics in various forms. Accordingly, there is a demand for the development of cosmetics made of natural materials that are environmentally friendly and nature-oriented, so there are few side effects, and all of them have excellent antibacterial, antioxidant, and anti-inflammatory effects, which are key factors for skin health, and can enhance skin vitality. .

KR 10-2015-0118078 A (2015.10.21.) KR 10-2015-0082813 A (2015.07.16.) KR 10-2015-0065560 A (2015.06.15.) KR 10-1458013 B1 (2014.10.29.)

Accordingly, the inventors of the present invention have made constant efforts to develop a cosmetic composition for enhancing skin vitality of a novel natural material. It has been treating wounds, and as a result of research in the field of medicine, Graviola extract containing phytochemical and acetogenin, which are anti-cancer and antioxidant substances, has a skin soothing effect, skin barrier strengthening, whitening and anti-aging effect, antibacterial activity, and skin cell proliferation enhancement effect, and to maximize these effects, an effective combination method of graviola extract, plant extract with skin stabilizing effect, and various peptides was identified, The present invention was completed by confirming the excellent skin vitality enhancing effect.

Accordingly, an object of the present invention is to provide a cosmetic composition for enhancing skin vitality comprising a graviola extract as an active ingredient.

In addition, another object of the present invention is to provide a method for producing a cosmetic composition for enhancing skin vitality containing an antibacterial extract of Graviola.

In addition, another object of the present invention is to provide an essence containing a cosmetic composition for enhancing skin vitality containing the graviola extract as an active ingredient.

In order to achieve the above object, the present invention is a graviola extract, portulaca extract, centella asiatica extract, witch hazel extract, bamboo extract, chamomile flower and leaf extract, rice bran extract, western hawthorn extract, eucalyptus leaf extract, grape leaf extract It provides a cosmetic composition for enhancing skin vitality comprising a graviola extract as an active ingredient.

At this time, the composition according to the present invention is 5 to 15% by weight of the graviola extract and the portulaca extract, centella extract, witch hazel extract, bamboo extract, chamomile flower and leaf extract, rice bran extract, western hazelnut, with respect to 100% by weight It is characterized in that it comprises 0.5 to 5% by weight of cypress tree extract, eucalyptus leaf extract, and grape leaf extract, respectively.

In order to achieve the above-mentioned other object, the present invention provides a method for producing a cosmetic composition for enhancing skin vitality containing an antibacterial extract of Graviola.

In order to achieve the above-mentioned other object, the present invention provides a skin essence comprising a cosmetic composition for enhancing skin vitality containing the Graviola antibacterial extract.

As described above, the present invention provides a cosmetic composition for enhancing skin vitality comprising an antibacterial extract of Graviola as an active ingredient and a manufacturing method thereof. The composition of the present invention has excellent antioxidant, antibacterial activity, skin barrier activity, whitening effect and anti-inflammatory activity, so it is effective in preparing essence for enhancing skin vitality such as skin improvement, skin soothing and skin moisturizing.

1 is a view showing a manufacturing process of a cosmetic composition for enhancing skin vitality containing an antibacterial extract of Graviola according to the present invention;
Figure 2 is a photo of the acne bacteria antibacterial test result of the cosmetic composition for promoting skin vitality containing the graviola antibacterial extract according to the present invention.

Hereinafter, detailed descriptions of preferred embodiments of the present invention will be described with reference to the accompanying drawings. Specific details have been shown in the following description, which are provided only to help a more general understanding of the present invention, and are not intended to limit the present invention to specific embodiments, and all changes included in the spirit and technical scope of the present invention, It should be understood to include equivalents or alternatives. In the description of the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the present application, they should not be interpreted in an ideal or excessively formal meaning. don't

"Skin vitality promotion" or "skin improvement" as used in the detailed description of the present invention means any action or effect that inhibits or delays skin damage or disease symptoms, improves disease symptoms, or improves skin health. means

The term “extract” refers to an active ingredient isolated from a natural product, that is, a material exhibiting a desired activity. The extract may be obtained through an extraction process using water, an organic solvent, or a mixed solvent thereof, and may include a dry powder thereof or any form formulated using the same. In addition, the extract includes fractionated extracts subjected to the extraction process.

1 is a view showing a method for manufacturing a cosmetic composition for promoting skin vitality containing an antibacterial extract of Graviola according to the present invention, as shown in FIG. A composition and a method for preparing the same are provided.

The Graviola extract, which accounts for the largest portion of the cosmetic composition for skin vitality enhancement containing the antibacterial Graviola extract according to the present invention, is Portuguese for Soursop (Soursop), which is anti-cancer and anti-oxidant phytochemicals It contains ingredients and acetogenin. The Graviola extract used in the present invention uses an extract obtained from the leaves and stems of Sueoshop. Graviola extract obtained from the leaves and stems of Sueo Shop is effective in soothing stressed or problematic skin and provides a refreshing feeling without stickiness after use.

The cosmetic composition of the present invention is an herbal plant extract as another active ingredient, portulaca extract, centella asiatica extract, witch hazel extract, bamboo extract, chamomile flower and leaf extract, rice bran extract, western hawthorn extract, eucalyptus leaf extract, grape Contains leaf extract.

The portulaca extract, centella asiatica extract, witch hazel extract, bamboo extract, chamomile flower and leaf extract, rice bran extract, western hawthorn extract, eucalyptus leaf extract, and grape leaf extract constituting the herbal plant extract used in the present invention are used for aromatherapy. It has a refreshing phytoncide fragrance to provide effects, and is intended to comfortably soothe skin irritated by stress.

The herbal plant extract is preferably extracted using a solvent selected from water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and more preferably ethanol or a mixture of ethanol and water. The suitable amount of the extraction solvent is not particularly limited, and is 1 to 10 times the weight of portulaca, centella, witch hazel, bamboo, chamomile flowers and leaves, rice bran, hawthorn, eucalyptus leaves, and grape leaves constituting oriental plants. , more preferably 5 to 8 times. However, the method of extracting the herbal plant extract, which is an active ingredient of the present invention, is not particularly limited.

As shown in Figure 1, the method for producing a cosmetic composition for enhancing skin vitality containing the antibacterial extract of Graviola according to the present invention is used as a moisture evaporation blocker as the first mixture 10 with respect to 100% by weight of the final composition 1-3% by weight of olive oil, 3-5% by weight of emulsifier, and 0.1-0.5% by weight of flavoring agent are added in this order, completely dissolved and dispersed (11), filtered, and transferred to a mixer (13) . Here, the emulsifier may be used by mixing polysorbate 80 and 60.

In the second mixture (20), 20-40% by weight of purified water used as a solvent, 0.1-0.5% by weight of each binder and emulsion stabilizer, and 1-3% by weight of trisiloxane are sequentially added, and then completely dissolved and dispersed (21). After filtering, it is transferred to an emulsifier (23). Herein, ethyl cellulose may be used as a binder, and xanthan gum may be used as an emulsion stabilizer.

The third mixture 30 contains 5-10% by weight of purified water as a solvent, 5-10% by weight of propylene glycol, 5-10% by weight of butylene glycol, 1-5% by weight of hyaluronic acid, hydrolyzed Decollagen 1-3 wt%, Glycerin 1-3 wt%, Surfactant 1-3 wt%, Sueoshop leaf/stem extract 5-15 wt%, Portulaca oleracea extract 0.5-5 wt%, Centella asiatica extract 0.5-5 wt%, Witch hazel extract 0.5-5% by weight, chamomile flower/leaf extract 0.5-5% by weight, bamboo extract 0.5-5% by weight, rice bran extract 0.5-5% by weight, hawthorn extract 0.5-5% by weight, eucalyptus leaf extract 0.5% by weight -5 wt%, grape leaf extract 0.5-5 wt%, sodium PCA 0.5-2 wt%, carboxymethyl chitin 0.5-2 wt%, acetyl hexapeptide 0.5-2 wt%, oligopeptide 0.5-2 wt%, dimethicone Chicon 1-3 wt%, 1,2-hexanediol 1-3 wt%, snail slime filtrate 0.5-2 wt%, allantoin 0.05-0.5 wt%, disodium EDTA 0.05-0.5 wt% were added in this order. After dissolving and dispersing (31), it is filtered and transferred to an emulsifier (33).

The first to third mixtures 10, 20, and 30 transferred to the emulsifier are completely mixed (40). When mixing, the rotation speed of the homo mixer is 1500-2500 rpm and the rotation speed of the paddle mixer is 20-50 rpm.

Then, the homo mixer is stopped and the paddle mixer starts defoaming by applying a vacuum while maintaining the rotation speed (41). The number of times of vacuum and defoaming can be performed 3-10 times.

The composition after vacuum and defoaming is placed in a storage tank, aged for 10 to 30 hours (43), and then transferred to and stored in a filling room (45).

As the surfactant included in the third mixture 30, PEG-60 hydrogenated castor oil may be used, and glycerin and sodium PCA are used as skin moisturizers. In addition, dimethicone and allantoin are used as skin protectants, 1,2-hexanediol is used as a preservative, and disodium idiate is used as a sequestering agent.

Fundamental factors of skin trouble, such as hormonal imbalance, bacteria in hair follicles, factors related to various stresses due to lifestyle and irregular eating habits, sensitivity of sebaceous glands, occurrence of secondary harmful bacteria due to hygiene conditions, inflammatory reactions, and oxidative stress When this increases, their actions act more complexly, resulting in allergic reactions, poor blood circulation, and cell metabolism and growth inhibition. And these causes eventually cause skin troubles such as itching and inflammation.

Inflammatory response, one of the above secondary factors, is caused by stimulation of harmful bacteria, and regardless of this, it is also chronically caused by metabolic syndrome caused by a westernized lifestyle characterized by high calories and lack of exercise, and mental stress or It can also be caused temporarily by shock. The inflammatory reaction accelerates tissue degeneration, suppresses cell growth, and overly sensitizes the immune system, further stimulating allergic phenomena, thereby damaging the health of skin tissue.

The above factors (harmful bacteria, inflammation, and oxidative stress) cause skin trouble by complexly and synergistically acting together to cause a vicious cycle that continues and worsens. Therefore, the composition of the present invention, which provides excellent effects on all of the above complex factors, improves the skin by sterilization, soothes the skin, and moisturizes the skin, as well as increases the synthesis of collagen, which is destroyed due to cell function decline, It promotes blood circulation and provides very excellent effects in preventing aging.

In addition, the conventional antibacterial agent should minimize the time it stays on the skin due to toxicity and irritation, and there is a problem of causing resistance. There is no problem even if formed, and it has a characteristic that resistance does not occur.

This point is well shown in the examples for evaluating the effectiveness of the cosmetic composition for enhancing skin vitality containing the antibacterial extract of Graviola according to the present invention.

Efficacy evaluation according to an embodiment of the present invention was conducted by antibacterial activity test, skin antibacterial peptide expression increase confirmation test, skin barrier resilience test, whitening effect confirmation test, anti-inflammatory effect confirmation test, and antioxidant effect confirmation test.

Example 1: Antibacterial activity test

The antibacterial activity test was conducted on the essence, which is a cosmetic composition containing the graviola extract according to the present invention treated with no preservatives. The control group was a product using ordinary purified water, and was carried out using Propionibacterium acnes to measure the sterilization effect. The test method was carried out according to the Halo test, in which a specimen was placed on a nutrient medium coated with acne bacteria and observed how much restraint band was generated around the specimen.

Treat 30 mg of the sample on a sterilized paper disc, place it in the center of the plate medium inoculated with the bacterial solution, and then incubate for 72 hours in anaerobic conditions using an anaerobic jar at (35 ± 1) Inhibition zone formed The diameter of was measured. As a result of measuring the antibacterial activity of the sample under this test condition, an 11 mm low zone was formed against the P. acnes bacteria used in the test.

In order to find out the antibacterial effect on the sample (essence), a halo test was conducted to measure the size of the inhibition zone. As a result, the sample formed an inhibition zone of 11 mm against P. acnes.

The antimicrobial activity test results are shown in Table 1 and FIG. 2 below. As shown in Table 1, the width (W) of the growth arrest zone is the difference between the total diameter (T) of the sample and the low zone and the diameter (D) of the sample divided by 2, and is 11 mm. Figure 2 shows a photograph after 72 hours of antibacterial activity test.

As described above, the cosmetic composition for enhancing skin vitality containing the antibacterial extract of Graviola according to the present invention has excellent antibacterial activity and can be effectively used in the preparation of functional essence for enhancing skin activity.

Example 2: Test for confirming the increase in the expression of antibacterial peptides in the skin

Graviola extract was treated with human keratinocytes (keratinocytes) to confirm the expression of antimicrobial peptides (CAMP; Cathelicidin AntiMicrobial Peptide, LL-37) expressed in skin cells themselves.

Example 3: Skin barrier resilience test

Graviola extract's ability to enhance cell proliferation was confirmed in keratinocytes and fibroblasts.

Example 4: Whitening effect confirmation test

The effect of inhibiting melanin production in melanosomes of melanocytes was confirmed.

Example 5: Anti-inflammatory effect confirmation test

In order to confirm the possibility of developing products for skin inflammatory diseases such as atopy and psoriasis, the effect of inhibiting mast cell degranulation and the production of inflammatory cytokines TNF-a and IL-8 of keratinocytes was confirmed.

Example 6: Antioxidant effect confirmation test

The free radical scavenging activity of the graviola extract was measured or the effect of increasing superoxide dismutase (SOD) activity in keratinocytes and fibroblasts was confirmed.

The cosmetic composition of the present invention can be prepared in any formulation conventionally prepared in the art, and in addition to the herbal plant extract of the present invention, topical application commonly used in the field of dermatology by containing a dermatologically acceptable medium or base Or it can be prepared in the form of an adjuvant that can be applied systemically.

In addition, the cosmetic composition of the present invention is a moisture evaporation blocker, binder, emulsion stabilizer, skin moisturizer, surfactant, preservative, skin protectant and metal ion sequestering agent or commonly used in cosmetics in addition to the graviola extract and herbal plant extract It may contain adjuvants commonly used in cosmetology or dermatology, such as any other ingredient. In addition, the above components may be introduced in an amount generally used in the field of dermatology. Meanwhile, the cosmetic composition of the present invention does not use parabens, which are cosmetic preservatives, at all.

In the essence using the cosmetic composition according to the present invention, the cosmetic composition for enhancing skin vitality may contain 5 to 15% by weight of graviola extract with respect to 100% by weight of the total product, and 0.5 to 5% by weight of herbal plant extracts, respectively. It is preferably included in a percentage by weight.

The essence of the present invention may further contain at least one active ingredient exhibiting the same or similar function in addition to the cosmetic composition for enhancing skin vitality.

On the other hand, as described above, the present invention provides a method for producing a cosmetic composition for enhancing skin vitality containing a graviola extract as an active ingredient, which includes dissolving the first to third mixtures separately, mixing them step by step, and cooling them do.

As described above, it was confirmed that the cosmetic composition of the present invention has excellent skin vitality enhancement effects such as antimicrobial activity, sterilization effect, skin barrier recovery ability, whitening effect, anti-inflammatory effect and antioxidant effect.

Meanwhile, in the detailed description of the present invention, specific embodiments have been described, but various modifications are possible without departing from the scope of the present invention. Therefore, the scope of the present invention should not be limited to the described embodiments and should not be defined, but should be defined by not only the scope of the claims to be described later, but also those equivalent to the scope of these claims.

As described above, the present invention provides a cosmetic composition for enhancing skin vitality containing a graviola extract as an active ingredient and a method for manufacturing the same, and the composition of the present invention has antioxidant, whitening, skin barrier recovery, antibacterial and anti-inflammatory activities This is excellent, so it is effective in manufacturing essence for enhancing skin vitality, so industrial applicability is high.

Claims (6)

A cosmetic composition comprising a graviola extract, portulaca extract, centella asiatica extract, witch hazel extract, bamboo extract, chamomile flower and leaf extract, rice bran extract, hawthorn extract, eucalyptus leaf extract, and grape leaf extract.
According to claim 1,
Based on 100% by weight of the composition, 5 to 15% by weight of the Graviola extract and the portulaca extract, centella extract, witch hazel extract, bamboo extract, chamomile flower and leaf extract, rice bran extract, western hawthorn extract, eucalyptus leaf extract , A cosmetic composition comprising 0.5 to 5% by weight of grape leaf extract, respectively.
An essence comprising the cosmetic composition of claim 1.
Based on 100% by weight of the final composition, 1-3% by weight of olive oil, 3-5% by weight of emulsifier, and 0.1-0.5% by weight of flavoring agent were sequentially added, and the first mixture, which was completely dissolved and dispersed and filtered, was mixed with an emulsifier (Mixer). ) The first step of transferring to;
20-40% by weight of purified water, 0.1-0.5% by weight of binder and emulsion stabilizer, and 1-3% by weight of trisiloxane are added in this order, completely dissolved and dispersed, and the second step of transferring the filtered second mixture to the emulsifier. ;
5-10% by weight of purified water, 5-10% by weight of propylene glycol, 5-10% by weight of butylene glycol, 1-5% by weight of hyaluronic acid, 1-3% by weight of hydrolyzed collagen, glycerin 1-3% by weight, 1-3% by weight of surfactant, 5-15% by weight of sorghum leaf/stem extract, 0.5-5% by weight of portulaca extract, 0.5-5% by weight of Centella asiatica extract, 0.5-5% by weight of witch hazel extract, Chamomile flower/leaf extract 0.5-5% by weight, bamboo extract 0.5-5% by weight, rice bran extract 0.5-5% by weight, hawthorn extract 0.5-5% by weight, eucalyptus leaf extract 0.5-5% by weight, grape leaf extract 0.5 -5 wt%, sodium PCA 0.5-2 wt%, carboxymethyl chitin 0.5-2 wt%, acetyl hexapeptide 0.5-2 wt%, oligopeptide 0.5-2 wt%, dimethicone 1-3 wt%, 1, 1-3% by weight of 2-hexanediol, 0.5-2% by weight of snail mucus filtrate, 0.05-0.5% by weight of allantoin, and 0.05-0.5% by weight of disodium EDTA were sequentially added, followed by dissolving and dispersing and filtering. 3 A third step of transferring the mixed agent to the emulsifier;
A fourth step of mixing the first to third mixing agents transferred to the emulsifier;
A fifth step of vacuuming and defoaming the mixture mixed in the fourth step; and
A method for producing a cosmetic composition, characterized in that consisting of a sixth step of transferring and storing the composition after vacuum and defoaming in the fifth step in a storage tank and aging for 10-30 hours.
The method of claim 4, wherein the fourth step,
A method for producing a cosmetic composition, characterized in that mixing the rotation speed of a homo mixer at 1500-2500 rpm and the rotation speed of a paddle mixer at 20-50 rpm.
The method of claim 5, wherein the fifth step,
The method for producing a cosmetic composition, characterized in that the homo mixer is stopped, and the paddle mixer performs vacuum and defoaming 3-10 times while maintaining the number of revolutions.

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