KR101583595B1 - Cosmetic composition and a method of manufacturing - Google Patents

Abstract

The present invention relates to a cosmetic composition and a manufacturing method thereof, and the cosmetic composition of the present invention includes a complex extract obtained from rooibos leaf, mulberry leaf, marine oil, cell vein, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato.
The present invention relates to a combination extract of rooibos, coriander, corn oil, cellve, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato which has excellent antioxidative, antiinflammatory and skin irritation-reducing effects, Is effective for use as a cosmetic for improving skin such as calming skin and relieving stress.

Description

[0001] Cosmetic composition and a method of manufacturing [

The present invention relates to a cosmetic composition and a manufacturing method thereof, and more particularly, to a cosmetic composition containing an active ingredient of a natural plant complex product having excellent antioxidant, anti-inflammation and skin irritation mitigating effect, and a manufacturing method thereof.

In general, harmful oxygen is a by-product generated during the process of making energy in the body, which is also referred to as active oxygen. Active oxygen is produced by the normal metabolism of oxygen and plays a major role in cell signaling and homeostasis.

However, due to environmental stress, the concentration of reactive oxygen species can increase dramatically and can play a major role in cell structure damage, which is oxidative stress. Too much active oxygen in the body damages cells and genes, promotes aging and causes various diseases directly or indirectly. Active oxygen has been mainly associated with cancer promoting processes and is known to promote cancer cell changes. However, recent research has shown that reactive oxygen species can alter genes and attack normal cell genes, causing inflammation of nearby cells, It is known to cause.

In addition, active oxygen acts as an immune function and a signal transducer to eliminate germs and foreign bodies, but when it occurs, it attacks the cells and breaks down the immune system and causes many illnesses. When UV rays are formed, melanin pigment is formed. As a result, when the metabolism is declined, spots and freckles are generated and active oxygen oxidizes the collagen constituting the skin. For this reason, collagen is weakened and the skin loses elasticity and wrinkles.

Therefore, active oxygen is also involved in inflammation reaction and immune system, and it is closely related to skin diseases as well as diseases, and thus efforts are being made to reduce active oxygen.

KR 2003-0017172 10 KR 2009-0099975 10 KR 2013-0040316 10

Accordingly, the present invention provides a method of screening herbal medicines having antioxidant and anti-inflammatory effects such as antioxidant and anti-inflammation, And a complex extract derived from tomato, which is excellent in antioxidant, anti-inflammatory and skin irritation-relieving effects.

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In order to achieve the above object, the present invention is characterized in that the present invention includes a complex extract comprising a combination of rooibos leaves, rhubarb, corn oil, cell bee, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato.

1 to 15 parts by weight of rooibos, 1 to 15 parts by weight of corn oil, 1 to 15 parts by weight of corn oil, 1 to 15 parts by weight of cell bee outpost, 1 to 15 parts by weight of red ginseng, 1 to 15 parts by weight of hibiscus 1 to 15 parts by weight of a leaf, 1 to 15 parts by weight of a red clover outpost, 1 to 15 parts by weight of a mangosteen fruit, 1 to 15 parts by weight of a cranberry, and 1 to 15 parts by weight of a tomato.

The complex extract may be selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diether ether And is extracted with at least one solvent.

The complex extract is characterized by comprising 0.1 to 10 parts by weight per 100 parts by weight of the total cosmetic composition.

1 to 15 parts by weight of rooibus leaves, 1 to 15 parts by weight of corn oil, 1 to 15 parts by weight of corn oil, 1 to 15 parts by weight of cell beer outpost, 1 to 15 parts by weight of red ginseng, 1 to 15 parts by weight of hibiscus leaf, 1 to 15 parts by weight of a red clover outpost, 1 to 15 parts by weight of a mangosteen fruit, 1 to 15 parts by weight of cranberry and 1 to 15 parts by weight of a tomato to prepare a plant mixture, The method comprising the steps of: preparing an extract by adding a solvent having a weight of 5 to 10 times the weight of the plant mixture; and concentrating and drying the extract prepared in the step.

Accordingly, the present invention relates to a combination extract of roots, roots, corn syrup, cell beer, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato, which are derived from natural sources and have excellent antioxidative, antiinflammatory, The cosmetic composition containing the extract as an active ingredient has an effect that it can be effectively used as a skin cosmetic composition for calming skin and alleviating stress.

Hereinafter, embodiments of the present invention will be described in detail.

The present invention relates to cosmetic compositions for improving skin comprising a complex extract comprising a combination of rooibos leaves, rhubarb, corn oil, cell bee, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato. The weight percentage of the complex extract is 1 to 15 < RTI ID = 0.0 > 1 to 15% by weight of corn oil, corn oil 1 to 15% by weight of cellulase outpost, 1 to 15% by weight of red ginseng, 1 to 15% by weight of hibiscus leaf, 1 to 15% by weight of red clover outpost, 1 to 15% by weight of mangosteen fruit, To 15% by weight and tomato 1 to 15% by weight.

Rooibos is a conifer belonging to leguminous plants and is a combination of 'rooi' and 'shrub' (bos) in African. Rooibos leaves are dried and used as tea. Tea is sweet, has no caffeine, tannin concentration is extremely low, and is considered to have antioxidant activity. Antioxidant ingredients of rooibos prevent cell destruction, prevent skin aging, contain minerals, soften skin, and are known to be good for cancer, allergy and atopy. In the present invention, various organs (such as leaves, flowers, stems and roots) of rooibos are dried, and more preferably rooibos leaves are dried.

The rhizome is a root shell of perennial peonies, which has a peculiar odor, a little bit of taste, and a very low quality. The pharmacological actions of the herringbone are analgesic, antipyretic, anti-convulsive, anti-inflammatory, anti-thrombotic, antiallergic, and antibacterial.

Cornus officinalis is the fruit of the Cornus officinalis, a small tall tree that leaves its leaves in the winter. In early spring, yellow and fragrant flowers are bloomed before the leaves are faded. Fruits are dried without food, dried or boiled in a car, and soaked in a drink. It is known to be effective for cholesterol reduction, skin care and diabetes, and is good for the kidneys and protects the legs. Therefore, it is widely used for the treatment of atopic diseases such as itching.

Shelvia (Salvia officinalis L.) is a perennial plant of the Mediterranean origin, also called sage. Medieval Selvia is also an ancient medicinal plant that has been widely known as a panacea since ancient times. Sage leaves are used as dried vine leaves and medicinal leaves, which contain essential oils. Its ingredients have excellent pharmacological effect on the nervous system and digestive system as well as the tonic action, and it is excellent as anti-inflammatory agent for various inflammation because it has disinfecting action such as preservation, antibacterial and anti-inflammation. In the present invention, leaves and flowers of cell beer, more preferably flowers are used as active ingredients.

Red ginseng is a kind of Chinese medicinal herb that ginseng root (Panax ginseng) is steamed. Red ginseng is known to be effective in the treatment of cancer and has effects such as anti-stress effect, blood circulation improvement, immune function and anti-aging. Red ginseng contains several kinds of phenolic compounds and maltose, so it has antioxidant and fatigue inhibiting effect and promotes the activation of enzymes related to inhibition of aging, thereby delaying aging. In addition, it is known that red ginseng has many saponins because it has immune function effectively. Therefore, it is known that it is effective in restoring immune function deterioration of a person having a weakened immune function or a weak person.

Hibiscus (Hibiscus mutabilis) is a herb belonging to the family Rosaceae, characterized by red petals. This red pigment contains a large amount of vitamin C, which contains a large amount of citric acid, a good and sour ingredient for skin, and is an herbal plant that attracts much attention as a fatigue remover. Hibiscus has an excellent antioxidant effect that keeps the blood clear and inhibits the generation and action of active oxygen causing skin aging. In the present invention, hibiscus flower or outpost, more preferably outpost, is used as an effective ingredient.

Red clover (Trifolium pratense L.) is a purple clover in flower-colored pink. It is a herb used as an herb from ancient Roman times. Flowers have anti-inflammatory and diuretic effects, so if you drink flowers with herbal tea, it will improve eczema and cough, and it is said to be effective for skin trouble and arthritis. In particular, it began to receive attention as a chemotherapeutic drug since about 1930, and it is now known to be used for chemotherapy. In the present invention, a red clover flower or an outpost, more preferably a plantlet is used as an active ingredient.

Mangosteen (Garcinia mangostana) is known to be effective against inflammation and antivirals with strong antioxidant power. The skin of mangosteen contains a component called xanthone or xanthone, which is a polyphenolic compound and is the world's best antioxidant. Mangosteen inhibits cell necrosis and cancer proliferation and helps improve blood circulation. It is also known to be effective for skin troubles such as immune enhancement, atopy, and allergy. It is also known that it contains various minerals such as calcium, phosphorus, and iron, vitamins, and a large amount of dietary fiber, and the flesh contains catechin components, thereby causing skin troubles and whitening effects. In the present invention, the whole mangosteen husk or fruit is used, and more preferably, the whole fruit is used as an effective ingredient.

Cranberry (Vaccinium oxycoccus L.) refers to the fruit of a group of plants belonging to the azalea and vaccinium. It is an evergreen thick-leaved tree, and is an alpine, Korean paper, and wetland type water. When the flower is bloomed, the petal turns upside down and dries. It is said that it is like the head and neck of the cranes. It is said that it contains benzoic acid abundantly, has anticancer effect, and strengthens immunity. It is known that it has more antioxidative and antioxidant effects and is rich in uronic acid, so it has the effect of reducing obesity and suppressing obesity.

Tomato (Solanum lycopersicum) refers to branches and branches or branches. Tomato red lycopene ingredient is good for anticancer effect and is known to be more than twice as strong as beta carotene ingredient known as anticancer drug. Vitamins A and C are abundant, and it is known to effectively treat and prevent adult diseases caused by cancer and other acidification. Because the moisture content of the tomato is 90%, it smoothes the metabolism and gives the rough skin. Vitamin B of the tomato makes skin and hair smooth and elastic, and vitamin E prevents aging.

According to one embodiment of the present invention, the combined extracts of rooibos, mulberry, coriander, svelea, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato may be hot water extract or water, ethanol, methanol, butanol, Is a solvent extract which is extracted with at least one solvent selected from the group consisting of glycerol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diether ether.

That is, the combined extracts of rooibos, mulberry, coriander, selenium, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato can be used in the form of a mixed extract obtained by extracting each of the components with the solvent.

Hereinafter, a method for producing a natural plant complex extract of the present invention will be described.

According to one embodiment of the present invention, firstly, rooibus leaves 1-15 1 to 15% by weight of corn oil, corn oil 1 to 15% by weight of cellulase outpost, 1 to 15% by weight of red ginseng, 1 to 15% by weight of hibiscus leaf, 1 to 15% by weight of red clover outpost, 1 to 15% by weight of mangosteen fruit, To 15% by weight of tomato and 1 to 15% by weight of tomato are mixed and mixed to prepare a natural plant mixture. (Step 1) Here, 1 to 15 parts by weight of the weight portion is a value accumulated through a lot of experiments and data.

Subsequently, an extract is prepared by adding a solvent having a weight of 5 to 10 times the weight of the crude drug mixture. (Step 2) If less than 5 times of the solvent is added, there is no effect of mixing the solvent properly. If the amount of the solvent is more than 10 times, the effect of the mixture is reduced due to excessive mixing.

The extraction solvent is at least one selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether One type of solvent may be used.

The extract solution extracted with the solvent can be concentrated and then dried to produce a natural plant complex extract (red complex). (Third step)

The combined extract of rooibosum, mulberry, corn oil, red bean, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato according to the present invention thus produced was superior in antioxidative activity by its synergistic action (Test Example 1) Cytotoxicity was alleviated from external stimuli due to irradiation (Test Example 2), and excellent effects were also shown in the effect of suppressing the expression of inflammation-related factors (Test Example 3). In addition, it was confirmed that the natural plant complex extract had excellent effects on the skin irritation-reducing effect (Test Example 4).

In the cosmetic composition of the present invention, the combined extract of rooibos, coriander, corn oil, cellve, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition. It is because of many experiments and accumulated data that the effect is excellent in the case of the weight part as described above.

The cosmetic composition containing the natural combination extract of rooibos, coriander, marinated oil, cellve, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato having the antioxidant and anti-inflammatory effects of the present invention can be produced in any formulations conventionally prepared Such as lotion, cream, essence, cleansing foam, cleansing water, pack, body lotion, body oil, body gel, shampoo, rinse, hair conditioner, hair gel, foundation, lipstick, mascara, .

Hereinafter, the present invention will be described in detail with reference to examples and test examples of the present invention, but the present invention is not limited thereto.

Example 1: Preparation of natural plant complex extract

The dried and cut rooibos leaves, Mulberry, Corn oil, Cellular outpost, Red ginseng, Hibiscus leaf, Red clover outpost, Mangosteen fruit, Cranberry and Tomato were mixed in the ratios shown in Table 1 below.

To the plant mixture, 70% aqueous ethanol (5 kg) (3 kg) was added as an extraction solvent, and the mixture was boiled at 100 for 8 hours in an extractor equipped with a cooling condenser. After filtering, the mixture was filtered through a 300-mesh filter paper, left at room temperature for 3 days, and the precipitate was filtered twice using filter paper Edbentek No. 5 and Watman GFC 47 mm filter paper. Then, the mixture was concentrated at 50 using a vacuum concentrator and then dried using a spray dryer (manufactured by BUCHI) to obtain 35.7 g of a natural plant complex extract.

Comparative Example 1 to Comparative Example 3: Preparation of a natural plant complex extract

The natural plant complex extract was prepared in the same manner as in Example 1, according to the ratios shown in Table 1 below. Natural product known to have antioxidant activity as Comparative Example 1, natural product known to have anti-inflammatory activity as Comparative Example 2, and natural product known to have anti-irritation activity as Comparative Example 3 were used.

[Table 1]

Example 2, Example 3: Preparation of lotion containing crude drug complex extract

Lotions containing roots, roots, coriander, cellulare, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato combined extract (Example 1) were prepared according to a conventional method with the composition and contents shown in Table 2 below .

[Table 2]

Test Example 1: Measurement of antioxidative effect using NBT method

In order to confirm the antioxidative effect of the extracts of roubicin, mulberry, corn oil, cellve, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato obtained in Example 1, , And the antioxidative activity was measured using the NBT method. In order to measure the antioxidative effect, the active oxygen produced by xanthine and xanthine oxidase was measured by the NBT method and the effect of the test substance on the removal of active oxygen, that is, the effect of active oxygen scavenging was evaluated. The active oxygen generated by xanthine and xanthine oxidase was reacted with Nitro Blue Tetrazolium (NBT), and the blue color produced thereby was measured at a wavelength of 560 nm to measure the active oxygen scavenging rate. The method of measuring the active oxygen scavenging rate is as follows. 2.4 ml of 0.05 M Na ₂ CO ₃ , 0.1 ml of a 3 mM xanthine solution, 0.1 ml of a 3 mM EDTA solution, 0.1 ml of a BSA solution and 0.1 ml of a 0.72 mM NBT solution were added to a vial (5 ml vial) and thereto was added DMSO 0.1 ml of the sample solution prepared at a concentration of 0.1 (wt / v)% is added and left at 25 for 10 minutes. Thereafter, 0.1 ml of xanthine oxidase solution is added, and the mixture is stirred rapidly and incubation is started for 25 to 20 minutes. Then, 0.1 ml of ₆ Mm CuCl ₂ solution is added to stop the reaction, and the absorbance St at 560 nm is measured. The blank test is carried out in the same manner as above using distilled water instead of the sample solution, and the absorbance Bt is measured. Again, the blank of the sample solution is measured by the same procedure using distilled water instead of the xanthine oxidase solution to determine the absorbance Bo. The results of the effects were calculated by the following formula, and the results are shown in Table 3 below.

Inhibition rate (%) = [1- (St-So) / (Bt-Bo)] 100

St: Absorbance at 560 nm after enzyme reaction of sample solution

Bt: Absorbance at 560 nm after enzyme reaction of blank test solution

So: the absorbance at 560 nm before the reaction in the absence of enzyme in the sample solution

Bo: absorbance at 560 nm before the reaction in the absence of enzyme in the blank test solution

[Table 3]

As shown in Table 3, the antioxidative effect was confirmed in all of the samples tested at a concentration of 0.1 (wt / v)%. In particular, the extract of natural combination of rooibos, rhododendron, corn oil, cellve, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato showed excellent antioxidative effect as compared with Comparative Example 1, Showed better antioxidant effect.

Test Example 2: Confirmation of mitigation effect of cytotoxicity by ultraviolet irradiation

Human-derived fibroblasts were cultured in 96-well plates at a concentration of 1 × 10 ⁵ cells / well, and 0.2 ml of IMDM medium containing 10% FBS was added and cultured in a CO ₂ incubator for 18 hours. After the sample solution dissolved in DMSO was treated, the amount of ultraviolet light of 2 MED was irradiated for 10 minutes and cultured for 24 hours. After that, MTT assay was performed to examine cell viability. For MTT assay, 0.1 ml of MTT solution was added to the cultured cells, followed by incubation for 4 hours. The medium was removed and 0.5 ml of DMSO was added to the formazan, and the absorbance was measured at 570 nm using an ELISA. BHT was used as a control.

[Table 4]

As shown in Table 4, the cytotoxic relaxation effect was confirmed in all of the samples tested at the concentration of 0.1 (wt / v)%. In particular, the extracts of natural combinations of rooibos, rhododendron, corn oil, cellve, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato showed better cytotoxic effects than those of Comparative Examples 1 to 3, Effect.

Test Example 3: Confirmation of suppression effect of inflammatory mediator expression by ultraviolet irradiation

RAW 264.7 cells were adjusted to 1 × 10 ⁵ cells / well using DMEM supplemented with 10% FBS, inoculated on a 24-well plate, treated with 2 MED for 10 min, Lt; / RTI > The amount of TNF-α produced was measured using a TNF-α ELISA kit (eBioscience INC, USA). The inhibition rate of TNF-? Production was calculated by the following equation, and the results are shown in Table 5 below.

TNF-production inhibitory rate (%) = [(amount of TNF-α production in control group - amount of TNF-α production in sample) / amount of TNF-α in control group] × 100

[Table 5]

As shown in Table 5, TNF inhibitory effect was confirmed in all of the samples tested at the concentration of 0.1 (wt / v)%. In particular, the extracts of natural combination of rooibos, rhododendron, corn oil, cellve, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato showed excellent TNF-α inhibitory effect in comparison with Comparative Examples 1 to 3.

Test Example 4: Confirmation of skin soothing effect

The present invention relates to a cosmetic composition containing a natural product fermented extract according to the present invention and a cosmetic composition containing the natural product fermented extract according to the present invention, The degree to which stimulation induced by applying a cosmetic composition not contained was calibrated relative to each other. First, taping was repeated 30 times with a scotch tape on both sides of the underbody to cause skin erythema. After the erythema was induced, the cosmetic containing the natural product fermented extract according to the present invention was applied, and the cosmetic without the natural product fermented extract according to the present invention was applied. The degree of skin soothing was evaluated 3 hours after application.

≪ Evaluation of degree of skin irritation (erythema) >

0 (no response) 1 (very mild erythema) 2 (faint or mild erythema)

3 (clear erythema but weak erythema) 4 (pronounced erythema) 5 (severe erythema)

[Table 6]

As can be seen in Table 6 above, the lotion compositions of Examples 2 to 3 containing the present invention rouibos, coriander, corn oil, cellve, red ginseng, hibiscus, red clover, mangosteen, cranberry, The skin irritation mitigation effect was superior to that of the control or non-extract containing control. Among them, the lotion formulation of Example 3 showed the best skin irritation mitigation effect.

Claims (5)

Wherein the cosmetic composition comprises a complex extract of a combination of roots, roots, roots, coriander, cellulare, red ginseng, hibiscus, red clover, mangosteen, cranberry and tomato.
The composition of claim 1, wherein the complex extract comprises 1 to 15 parts by weight of rooibus leaves, 1 to 15 parts by weight of cornstalks, 1 to 15 parts by weight of cornstarch, 1 to 15 parts by weight of cellulite outpourings, 1 to 15 parts by weight of red ginseng, 1 to 15 parts by weight of a hibiscus leaf, 1 to 15 parts by weight of a red clover outpost, 1 to 15 parts by weight of a mangosteen fruit, 1 to 15 parts by weight of a cranberry, and 1 to 15 parts by weight of a tomato.
The method of claim 1, wherein the combined extract is selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diether ether Wherein the cosmetic composition is extracted with at least one solvent selected from the group consisting of
The cosmetic composition according to claim 1, wherein the complex extract is 0.1 to 10 parts by weight based on 100 parts by weight of the total cosmetic composition.
1 to 15 parts by weight of rooibus leaves, 1 to 15 parts by weight of corn oil, 1 to 15 parts by weight of corn oil, 1 to 15 parts by weight of cell beer outpost, 1 to 15 parts by weight of red ginseng, 1 to 15 parts by weight of hibiscus leaves 1 to 15 parts by weight of a red clover outpost, 1 to 15 parts by weight of mangosteen fruit, 1 to 15 parts by weight of cranberry and 1 to 15 parts by weight of tomato,
A second step of preparing an extract by adding a solvent having a weight of 5 to 10 times the weight of the plant mixture prepared in the first step;
And a third step of concentrating and drying the extract prepared in the second step.