KR20130040316A - Cosmetics composites - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a Rhododendron brachycarpum extract is provided to relieve wrinkling and to enhance cell proliferation. CONSTITUTION: A cosmetic composition for improving skin condition contains 0.001-20% of a Rhododendron plant extract. Rhododendron plants include Rhododendron brachycarpum G. Don. and Rhododendron brachycarpum G. Don var. roseum koidz. The cosmetic composition for antioxidation, anti-wrinkling, and cell proliferation contains the extract. The extract is prepared using one or more solvents selected from the group consisting of water including purified water, methanol, ethanol, propylene glycol, ethyl acetate, butylene glycol, ether, chloroform, hexane, ethyl acetate, and acetone. The cosmetic composition for whitening and antioxidation is manufactured in the form of a skin softener, a lotion, a nutrition cream, a massage cream, a nutrition serum, an essence, or a pack.

Description

Cosmetic composition for skin improvement containing manchurian extract {cosmetics composites}

The present invention relates to a cosmetic composition containing wrinkles (Rhododendron brachycarpum) extract to improve wrinkles and skin having an anti-wrinkle effect and cell proliferation effect.

Human skin is an organ that tends to change as it ages, along with eyes and teeth. These skins are exposed to ultraviolet light, and photochemical reactions that produce reactive oxygen species continue to occur. These reactive oxygen species also lead to skin cell and tissue damage when the skin becomes inflamed. They break the antioxidant defenses, which consist of antioxidant and non-enzymatic antioxidants, bringing the oxidant / antioxidant balance into the oxidized state. In skin, as a means to defend against these exogenous factors, antioxidants such as vitamin E, vitamin C, ubiquinol and glutathione, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (catalase) Antioxidants such as CAT. However, continuous exposure of exogenous factors such as UV results in the reduction of antioxidants and the destruction of antioxidant enzymes, which are the skin's defense system, resulting in oxidative stress, damaging cellular components and promoting photoaging (Sies, H. ( 1986) Biochemistry of oxidative stress. Angew. Chem. 25, 1058-1071., Sies, H. (1991) Oxidative stress: Oxidants and antioxidant s. Academic Press, N. Y.). Recently, in order to protect the skin from such exogenous factors, studies of sunscreens and antioxidants have been actively conducted. As a result, continuous oxidative stress may lead to lipid peroxidation, protein oxidation, activation of proteolytic enzymes that destroy epileptic components, chain cleavage and abnormal cross-linking of elastic fibers collagen and elastin, cleavage of hyaluronic acid chains, promotion of melanogenesis, DNA Causing damage to biological components such as oxidation. As a result, skin aging, characterized by decreased elasticity, wrinkles and spots, and freckles, is accelerated.

 External signs of skin aging include increased skin wrinkles, relaxation, reduced gloss, gloss, and smoothness, and reduced elasticity. The texture becomes rough, wrinkles are disturbed, pigmentation spots increase, partial depigmentation spots occur, and yellowish color occurs. Such aging can be divided into natural aging and photo aging. The skin change caused by ultraviolet rays is called photo aging, and the change according to age appearing in the area that is hardly exposed to sunlight is called natural aging.

Although the rate of aging of the skin may be changed by the presence or absence of ultraviolet rays, the result suggests that human skin has no choice but to age. Therefore, in order to prevent skin aging, a system capable of suppressing excess free radical species generation and efficiently removing the generated free radicals, and a system related to the inhibitory activity of enzymes causing wrinkles on the skin, must be included in the prescription of cosmetics. have.

  Plants in the genus include Rhododendron brachycarpum G. Don, Rhododendron brachycarpum G. Don var.roseum koidz or Yellow Rhododendron brachycarpum G. Don, and Rhododendron brachycarpum G. Don. It is distributed in Japan. It is an alpine plant in deep mountain forests almost everywhere in our country. It is an evergreen shrub. The bark is white with ash. Height 4m, leaves regenerated, end of branch, skin, oval, elliptical lanceolate, dull, 8-20cm long, 2-5cm wide, curled back, flat, dark green, glossy Its back is dense with light brown hairs. Flowers bloom in June-July and run by inflorescences at the end of 10-20 branches. Petioles reddish brown with dense hairs. Corollas are funnel-shaped, white or light yellow, with green spots on the inside, with five branches. There are 10 surgeries and 1 pistil. The ovary has dense brown hairs. Fruits are granules, oval-shaped, about 2cm long, ripen brown in September. The components of the Rhododendron include andromethotoxin, flavonoid quercetin, hyperpherides, phenolic glycosides, rhododendrin and its aglycone, rhododendrol, tannin, and piperine. Leaves are called Seoknam-Yeop (石 南 葉) and are used for the treatment of urinary tract pain, pain, arthralgia, renal low back pain, sonic, menstrual irregularities, infertility, neuralgia, hypertension, tonic, diuretic It works as well, and was used for earaches, phlegm and abdominal pain. (Ingredients and Uses of Medicinal Herbs, Kwan-Shim, p543, 1999), (Principal Color Book of Korean Plants, Kyohaksa, 579p (1998))

It is an object of the present invention to provide a cosmetic composition for improving wrinkles and skin having a rhododendron brachycarpum extract and having an anti-wrinkle effect and a cell proliferation effect.

The present invention is a cosmetic composition for improving skin containing the extract of Rhododendron plants.

According to the present invention is provided with a cosmetic composition for improving wrinkles and skin having a rhododendron brachycarpum extract having a wrinkle relaxation effect and cell proliferation effect.

 In order to achieve the above object, the result of searching for an effective material for wrinkling and cell proliferation by having a function of preventing or improving skin aging among various natural products was found to have an excellent effect of preventing and improving skin aging. In the present invention, the anti-wrinkle effect, cell proliferation effect of the extract of Rhododendron was measured. As a result, the anti-aging effect of the skin and the skin improvement effect were found. At the same time, the use of non-toxic plants has solved the safety and safety issues.

The process of preparing the Rhododendron extract of the present invention is as follows. In order to increase the content of the active ingredient extracted in step 1, and step 1 to extract the active ingredient using an extract solvent, simply concentrated under reduced pressure or concentrated under reduced pressure after fractionating the aqueous layer and the non-aqueous layer using an organic solvent. It consists of two steps.

The present invention is described in more detail as follows. Firstly, the extract is a solvent, water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, chloroform, glycerin, butylene glycol, propylene glycol, hydrous ethanol, hydrous methanol, hydrous propanol, hydrous butanol, hydrous 1-20 times by volume of one or more solvents selected from the group consisting of glycerin, hydrous butylene glycol and hydrous propylene glycol are added. Extraction method is a method of extracting by heating 40 ~ 95 ℃, 4 to 20 hours in the state of preventing the evaporation of the solvent is equipped with a cooling condenser, or by extracting the active ingredient by deposition for 1 to 15 days at 5 ~ 40 ℃ Can be. The extract of this extract is concentrated in full pressure under reduced pressure while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser, and then added to the cosmetics in an amount of 0.01 to 10%, preferably 0.01 to 5% by dry weight. . The following Examples and Experimental Examples illustrate the content of the present invention, but the content of the present invention is not limited thereto.

Example  One.

1kg of Manbyeongcho (outpost) purchased from Gyeongdong Market, Seoul, was put in 10L of 95% ethanol, heated and extracted for 5 hours in a 70 ° C water bath equipped with a cooling condenser, filtered through a 250 mesh filter cloth, and left to mature at 4 ° C for 7 days. Then, the resultant was filtered with Whatman filter paper to obtain 8.7L of extract (68.8 g of solid).

Example  2 ~ 17

 Using the solvent of Table 1 below and extracting in the same manner as in Example 1 and the results are shown in Table 1 below.

Solvent (w / w%) Extract (L) Solids (g) Example 2. water 8.6 72.3 Example 3. Hexane 8.5 71.5 Example 4. Ethyl acetate 8.5 68.3 Example 5. chloroform 8.3 66.2 Example 6. Acetone 8.5 63.5 Example 7. Function Acetone (70) 8.5 64.8 Example 8. Butanol 8.7 66.8 Example 9. Water Butanol (70) 8.7 70.3 Example 10. Methanol 8.6 71.5 Example 11. Water Methanol (70) 8.6 72.6 Example 12. ethanol 8.5 74.5 Example 13. Functional Ethanol (30) 8.5 76.3 Example 14. Functional ethanol (70) 8.7 77.8 Example 15. Butylene glycol 8.7 72.1 Example 17. Hydrated butyl glycol (30) 8.5 73.5 Example 18. Hydrate Butylene Glycol (70) 8.4 70.0 Example 19. Propylene glycol 8.6 71.0 Example 20. Hydrous propylene glycol (70) 8.5 71.5 Example 21. glycerin 8.5 68.5 Example 22. Functional glycerin (70) 8.6 66.3

Example 23.

1 kg of Manbyeongcho (outpost) purchased from Gyeongdong Market, Seoul, was added to 10L of 30% hydrous ethanol, stirred and extracted at room temperature for 3 days, filtered through 250 mesh filter cloth, left to mature at 4 ° C for 7 days, and then filtered through Whatman filter paper. 8.3 L of an extract (solid content of 65.8 g) was obtained.

Example  24.

1kg of Manbyeongcho (outpost) purchased from Gyeongdong Market, Seoul, was added to 10L of 30% hydrous butylene glycol, stirred and extracted at room temperature for 3 days, filtered through a 250 mesh filter cloth, left to mature at 4 ° C for 7 days, and then filtered through Whatman filter paper. To give 8.6L (solid content 63.8g).

Example  25.

53 g of the extract powder obtained in Example 14 was dispersed in 400 g of water, 400 g of n-hexane was added thereto, and shake extraction was performed three times. The n-hexane layer was filtered and concentrated under reduced pressure. 4.4 g of extract powder was obtained.

Example  26.

400 g of ethyl acetate was added to 400 g of the aqueous layer obtained in Example 25, followed by shaking extraction three times. The ethyl acetate was filtered and concentrated under reduced pressure. 11.8 g of extract powder was obtained.

Example  27.

400 g of butanol was added to 400 g of the water layer obtained in Example 26, followed by shaking extraction three times. The butanol was filtered and concentrated under reduced pressure. 20.1 g of extract powder was obtained.

Example 28 .

400 g of the water layer obtained in Example 27 was concentrated under reduced pressure to obtain 12.5 g of an extract powder.

Experimental Example  One. free Radical  Erasure efficacy experiment

   Using the obtained samples of Examples 1 to 28 to improve the method of Fujita, etc., using a stable 1,1-diphenyl-2-picrylhydrazyl radical (1,1-Diphenyl-2-picrylhydrazyl radical) The free radical scavenging activity was measured. 2 ml of the sample was added to 1 ml of 0.2 mM diphenyl-2-picrylhydrazyl (DPPH) solution at each concentration, reacted at room temperature for 10 minutes, and then the maximum absorbance was measured at 525 nm according to the following formula. The free radical scavenging effect (%) was obtained.

Superoxide radical scavenging activity SC ₅₀ is the concentration of extract (μg / ml) required to remove 50% of the superoxide radical.

% Free radical scavenging capacity = [1- (A-A ') / (B-B'))] x 100

A: absorbance at 525 nm of the reaction solution to which the sample was added

A ': absorbance at 525 nm of the reaction solution without DPPH

B: absorbance at 525 nm of reaction solution without sample

B ': absorbance at 525 nm of sample and reaction solution without DPPH

Free radical scavenging effect Example SC _{50 (} μg / ml) Example 1 1 21 Example  2 103 Example  3 99 Example  4 98 Example  5 102 Example  6 113 Example  7 152 Example  8 152 Example  9 230 Example  10 165 Example  11 89 Example  12 99 Example  13 86 Example  14 67 Example  15 156 Example  16 252 Example  17 232 Example  18 167 Example  19 151 Example  20 160 Example  21 135 Example  22 125 Example  23 103 Example  24 72 Example  25 110 Example  26 65 Example  27 30 Example  28 135

Experimental Example 2 . Elastase  Inhibitory effect experiment

     The inhibitory effect of elastase activity was measured on the Rhododendron extract of Examples 1 to 28. Substrate solution containing 8.8 mM Elastase substrate Succ-Ala-Ala-A-p-nitroanilide standard in 60 µl of a buffer adjusted with 0.267 M Tris solution with 0.267 M hydrochloric acid (HCl) to pH 8.0. 100 μl of the sample prepared by diluting the extract obtained in Examples 1 to 28 to a predetermined concentration in purified water was mixed and mixed with each other, and 10 μg / ml of Porcine Pancreatic Elastase standard was used. After adding 20 μl of the enzyme solution, the mixture was reacted at 25 ° C. for 15 minutes, and the absorbance B was measured at 410 nm for the amount of para-nitroaniline produced.

 In addition, 100 µl of each sample prepared by adding 20 µl of substrate solution, 20 µl of purified water and 20 µl of purified water, and the extract water obtained in Examples 1 to 23 was added to 60 µl of the same buffer as described above. After that, the absorbance C was measured as a control.

In addition, absorbance A was measured using 100 μl of purified water instead of the sample as the test solution, and the absorbance A was measured using 120 μl of purified water instead of the enzyme solution and the test solution. Was taken as the color correction liquid and the absorbance D was measured to obtain an elastase inhibition rate (%) as shown in Equation 1 below.

[Equation 1]

Elastase Inhibition Rate (%) = [1- (Enzyme Activity (BC) / Control Enzyme Activity (AD) of Each Sample]] × 100

Elastase inhibitory activity IC ₅₀ for each example was expressed as the concentration of extract (μg / ml) required to inhibit the elastase activity by 50%.

As a result of the above experiment, the lowest elastase inhibitory activity (IC ₅₀ = 620 µg / ml) for the extract obtained in Example 27 as shown in Table 3 below, for the extract obtained in Example 27 The highest elastase inhibitory activity (IC ₅₀ = 75 μg / ml) was confirmed.

Elastase Inhibitory Effect Example IC ₅₀ (μg / ml) Example 1 240 Example 2 260 Example 3 250 Example 4 255 Example 5 260 Example 6 195 Example 7 165 Example 8 170 Example 9 165 Example 10 175 Example 11 145 Example 12 165 Example 13 160 Example 14 140 Example 15 165 Example 16 155 Example 17 170 Example 18 169 Example 19 150 Example 20 195 Example 21 200 Example 22 195 Example 23 200 Example 24 185 Example 25 350 Example 26 90 Example 27 75 Example 28 620

Experimental Example 3. Experiment of cell proliferation effect in monolayer cell culture

The cell proliferation effect of the extract obtained in Examples 1 to 28 was measured by applying the method described in the literature (MTT assay, J Immunological Methods 65, 55 (1983)).

Human fibroblasts (ATCC, CRL-2310) were seeded in 24 well plates at a concentration of 1 × 10 ⁴ cells / ml. The medium used was DMEM (Dubelcco's Modified Eagle Medium, BRL, USA) medium containing 10% bovine serum, and DMEM (Dubelcco'S Modified Eagle Medium, BRL, USA) containing 2% bovine serum 24 hours after cell inoculation 1 10 ml of the extracts obtained in Examples 1 to 28 were added at 100 mg / ml and 200 mg / ml, followed by incubation in a 37 ° C., 5% CO ₂ incubator for 2 days. After the incubation, 2.5 mg / ml yellow tetrazolium salt (MTT) solution was added 1.0 ml per well, and after 4 hours, the yellow tetrazolium salt solution was removed, and dimethyl sulfoxide (DMSO) was added 1.0 ml per well. After dissolving for minutes, the absorbance was measured at 570 nm. In addition, the solvent of the extract as a negative control group was carried out in the same manner as described above to compare and observe the effect of cell proliferation from the difference in relative absorbance.

As a result of the above experiment, as shown in Table 4 below, in the case of 100 µg / ml concentration of the extract obtained in each of Examples 1 to 28, Example 25 had the lowest cell proliferation effect of 95%. It showed the highest cell proliferation effect of 123% and an average cell proliferation effect of 116.3%. In the case of 200㎍ / ㎖ concentration of the extract, Example 25 was the lowest at 98%, and Example 27 was the highest at 135%. The cell proliferation effect was shown, and the average cell proliferation effect was 115.5%.

Example Treatment concentration (㎍ / ㎖) Cell proliferation effect (%) Example 1 100 109 200 121 Example 2 100 103 200 107 Example 3 100 111 200 118 Example 4 100 102 200 109 Example 5 100 103 200 107 Example 6 100 111 200 121 Example 7 100 103 200 121 Example 8 100 105 200 117 Example 9 100 103 200 114 Example 10 100 105 200 117 Example 11 100 106 200 112 Example 12 100 105 200 121 Example 13 100 108 200 117 Example 14 100 105 200 108 Example 15 100 107 200 108 Example 16 100 103 200 111 Example 17 100 109 200 118 Example 18 100 103 200 116 Example 19 100 107 200 115 Example 20 100 105 200 115 Example 21 100 105 200 121 Example 22 100 107 200 115 Example 23 100 108 200 119 Example 24 100 102 200 103 Example 25 100 98 200 95 Example 26 100 108 200 132 Example 27 100 120 200 135 Example 28 100 116 200 121

Prescription example  Criteria for dispensing

The extract used in the following formulation example was used by mixing the sample of Example 27 showing the best effect in the above example to 1% by weight in 50% hydrous butylene glycol.

Prescription Example 1.

A prescription example of a lotion (skin) among cosmetics containing Mannachocho extract is as follows. Here, the Rhododendron extract is that of Example 27. Table 5 is Prescription Example 1.

number Raw material Example 1 Comparative Formulation Example 1 One   Panacea Extract 10.0 - 2 Propylene glycol 5.00 5.00 3 ethanol 3.00 3.00 4 Imidazolidinylurea 0.20 0.20 5 Allantoin 0.20 0.20 6 Citric acid 0.20 0.20 7 Sodium citrate 0.12 0.20 8 Hydrogenated Castor oil 0.10 0.20 9 Methylparaben 0.10 0.20 10 Combination fragrance 0.10 0.10 11 Purified water Total 100 Total 100

<Manufacturing method>

Dissolve hydrogenated castor oil, methylparaben and combination chemicals in ethanol and add the remaining ingredients to purified water.

Prescription example  2.

Prescription examples of lotions in cosmetics containing panacea extract are as follows. Here, the Rhododendron extract is that of Example 27. Table 6 is prescription example 2.

number Raw material Execution Formulation Example 2 Comparative Formulation Example 2 One   Panacea Extract 10.0 - 2 Squalane 3.50 3.50 3 Cetyl alcohol 3.00 3.00 4 glycerin 3.00 3.00 5 Glyceryl stearate 2.50 2.50 6 Cetyl octanoate 6.00 6.00 7 Sorbitan Sesquioleate 1.50 1.50 8 Fiji-100 Stearate 1.00 1.00 9 Sheer butter 0.50 0.50 10 Carbomer 0.30 0.30 11 Triethanolamine 0.30 0.30 12 Methylparaben 0.20 0.20 13 Imidazolidinylurea 0.15 0.15 14 Propylparaben 0.10 0.10 15 Combination fragrance 0.10 0.10 16 Purified water Total 100 Total 100

<Manufacturing method>

The raw material of the above-described content is dissolved in oil by the general emulsification method as the oil phase component, the component dissolved in purified water as the water phase component, the water phase component and the oil phase component at a constant temperature (80 ~ 85 ℃) as a general homo mixer After emulsifying the homogeneous mixture, the thickener (carbomer) is neutralized, followed by cooling, fragrance, addition of plant mixture extract, and degassing.

Prescription Example 3

A prescription example of a cream in cosmetics containing Mannachocho extract is as follows. The mixed plant extract here is that of Example 27. Table 7 is prescription example 3.

number Raw material Example 3 Comparative Formulation Example 3 One   Panacea Extract 10.0 - 2 glycerin 6.00 6.00 3 Squalane 6.00 6.00 4 Isohexadecane 5.50 5.50 5 Cetyl octanoate 4.00 4.00 6 Glyceryl stearate 2.50 2.50 7 Polysorbate 60 1.00 1.00 8 Fiji-100 Stearate 1.00 1.00 9 Stearic acid 1.00 1.00 10 lanolin 1.00 1.00 11 Laureth-12 1.00 1.00 12 Cetyl alcohol 0.50 0.50 13 Sorbitan cisquioleate 0.30 0.30 14 Carbomer 0.30 0.30 15 Triethanolamine 0.30 0.30 16 Methylparaben 0.20 0.20 17 Imidazolidinylurea 0.15 0.15 18 Propylparaben 0.10 0.10 19 Combination fragrance 0.10 0.10 20 Purified water Total 100 Total 100

<Manufacturing method>

The raw material of the above-described content is dissolved in oil by the general emulsification method as the oil phase component, the component dissolved in purified water as the water phase component, the water phase component and the oil phase component at a constant temperature (80 ~ 85 ℃) as a general homo mixer After emulsifying the homogeneous mixture, the thickener (carbomer) is neutralized, and then cooled, flavored, added to the plant mixture extract, and degassed to complete the preparation.

[Anti-Aging Confirmation Experiment on Skin]

Experimental Example 4. Increased elasticity test on the skin

To measure the effect of increasing the elasticity of the skin when the lotions of Example 2 and Comparative Formula 2 were applied to the skin, 20 to 55 year old females face the normal, oily, dry and combination skin. In the left part, the nutrition lotion of Example 2 was applied, and in the right part of the face, the nutrition lotion of Comparative Example 2 was applied twice a day in the morning and evening, and after 1, 2, 3, and 4 weeks Skin elasticity was measured using a skin elasticity measuring instrument (Ballistometer). The measurement results are shown in Table 8 below.

Cosmetics of Example 2 Cosmetics of Comparative Formulation Example 2 ratio
The T ₀ T _One T ₂ T ₃ T ₄ T ₀ T _One T ₂ T ₃ T ₄ burnt
Power
castle Spring water 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 camp
width first 1.9 4.8 5.0 5.1 5.6 1.9 3.0 3.3 3.6 3.6 second 1.3 4.6 5.2 5.2 5.8 1.6 2.0 2.3 2.5 2.9 third 0.0 2.4 3.6 3.7 3.7 0.0 0.0 1.9 2.5 2.5 fourth 0.0 1.0 2.9 3.3 3.5 0.0 0.0 0.0 0.0 0.0 Average 0.80 3.20 4.17 4.33 4.65 0.88 1.25 1.88 2.15 2.25

(Note) T ₀ ; Before use

T ₁ ; After 1 week

T ₂ ; After 2 weeks

T ₃ ; After 3 weeks

T ₄ ; After 4 weeks

As can be seen from the above results, the skin elasticity effect is improved by the skin condition improvement effect compared to the nutrient lotion of Comparative Formulation Example 2 prepared without adding the nutrient lotion of Example 2 containing the plant mixture extract according to the present invention. It can be seen that it is very excellent.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but on the contrary, &Lt; RTI ID = 0.0 &gt; and / or &lt; / RTI &gt;

Claims (10)

A cosmetic composition for skin improvement containing extracts of Rhododendron plants.
The method of claim 1,
Plants of the genus Rhododendron brachycarpum G. Don, Rhododendron brachycarpum G. Don var.roseum koidz is a cosmetic composition for skin improvement.
The method of claim 1,
Plant of the genus Pleurotus is a cosmetic for improving skin, characterized in that yellow Rhododendron aureum Geori.
According to claim 1, Cosmetic composition having an antioxidant effect comprising an extract extracted from Rhododendron.
The method of claim 1,
A cosmetic composition having an anti-wrinkle effect, comprising an extract extracted from Rhododendron.
The method of claim 1,
Cosmetic composition having a cell proliferation effect, including the extract extracted from Rhododendron.
The method of claim 1,
The extract is at least one solvent selected from the group consisting of C1 to C4 lower alcohol, propylene glycol, ethyl acetate, butylene glycol, ether, chloroform, hexane, ethyl acetate, acetone, including purified water, methanol, ethanol, and the like. Extracted cosmetic composition.
The method of claim 7, wherein
Cosmetics, characterized in that the extract is obtained by immersing at 4 ~ 40 ℃ for 1 to 15 days or by heating for 2 to 20 hours at 40 ~ 100 ℃.
The cosmetic composition according to claim 1, which is contained in an amount of 0.001-20%, preferably 0.01-10%, as its dry weight.
The method of claim 1,
Cosmetic composition is a cosmetic composition for whitening and antioxidant in the form of supple cosmetics, nourishing cosmetics, nutrition lotion, nutrition cream, massage cream, nutrition serum, essence or pack.