KR100825450B1 - Skin anti-wrinkle cosmetics composition containing Forsythiae Fruit extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin wrinkles, characterized in that it comprises a soft extract, more preferably, matyrethinol, actinigen or a mixture thereof. The cosmetic composition for skin wrinkle improvement according to the present invention exhibits excellent effects in antioxidant, skin cell proliferation, collagen synthesis and collagenase expression related to skin wrinkle improvement and is very effective for skin wrinkle improvement, when applied to skin. It is very safe with no side effects.

Duct Extract, Matyr Resinol, Actigenin, Wrinkle Improvement

Description

Skin anti-wrinkle cosmetics composition containing Forsythiae Fruit extract}

The present invention relates to a cosmetic composition that is safe in skin application and has an excellent skin wrinkle improvement effect.

The skin has many opportunities for contact with a variety of external stimuli, making wrinkles more prone than other organs. Among them, facial skin is directly exposed to sunlight, dry air, and pollutants, so that aging such as wrinkles occurs earlier than skin that is not.

The most characteristic change due to the aging of the skin tissue is a change in the matrix (matrix), the aging of the human skin fibroblast (human skin fibroblast) is reduced the ability to produce fibers and substrates. The overall amount of substrate decreases, making the skin thinner and reducing the elasticity of the skin, forming wrinkles. In other words, as aging progresses, the skin becomes less elastic, impaired blood circulation, and weakened skin barriers.

In addition, when the skin is exposed to ultraviolet rays, free radicals and reactive oxygen species (ROS), which are the main causes of skin cell damage, are produced. They can also damage wrinkles by attacking collagen and fibers that cause DNA damage, attack cell membranes, create aging spots, and moisturize, soften, and flexibly maintain skin.

Many cosmetics have been studied to reduce skin wrinkles caused by such external environment or internal mechanism. In the recent development of cosmetics, natural herbal products are used to provide nutrition to the skin, minimize the toxic effects of cosmetics by chemical synthesis, reduce side effects, and treat the various symptoms of the skin. There is a growing interest in cosmetic development.

Therefore, the technical problem to be achieved by the present invention is to provide a cosmetic composition that is useful for skin wrinkle improvement due to the effects of antioxidant, cell proliferation, collagen synthesis, and collagenase expression inhibiting skin wrinkle improvement without causing skin side effects will be.

In order to achieve the above technical problem, the present invention provides a cosmetic composition for improving skin wrinkles, comprising a Forsythia Fruit extract.

Preferably, the present invention provides a cosmetic composition for improving skin wrinkles, which comprises matairesinol, actigenin, or a mixture thereof, more preferably matairesinol.

More preferably, the present invention provides a cosmetic composition for improving skin wrinkles, characterized in that the total content of matyresinol, actigenin or a mixture thereof in the cosmetic composition is 0.001 to 10% by weight relative to the total weight of the cosmetic composition. .

The present invention also provides a method for producing a soft duct extract having an increased content of the active ingredient for skin wrinkle improvement by treating the soft duct extract with an acid or an enzyme.

Hereinafter, the cosmetic composition for improving skin wrinkles of the present invention will be described in more detail.

The present invention is based on the surprising finding that duct extracts, in particular matyrethinol and / or actinigen contained in ducts, are safe and useful for skin wrinkle improvement.

Forsythia fruit of the present invention is a fruit of Forsythia viridissima L. belonging to the family Oleaceae ( Oleaceae ). Fruits are narrow egg-shaped, with short beak at the end, and split into 2 pieces when fully cooked. Usually, when the fruit is ripe, pick it up and dry it in the sun.

These associations have been used as anti-inflammatory and drainage skin diseases in oriental medicine, and as a tyrosinase inhibitor, inhibit the melanin synthesis and act on melanin metabolic pathways induced by α-melanosite stimulating hormone (α-MSH). It has the effect of controlling melanogenesis and is attracting attention as a material for whitening cosmetics. It is also known that it can be used as a medicine for treating cancer.

As described above, yeonkyo is widely used as a new material for food, cosmetics, and medicines, but it is not known about the cosmetic composition for improving wrinkles and skin wrinkles containing such yeonkyo extract. Accordingly, the present inventors completed the present invention by evaluating antioxidant, skin cell proliferation, collagen synthesis, and collagenase expression inhibitory effects associated with skin wrinkle improvement.

As a result of the various evaluation experiments, among the substances extracted and separated from the ducts, matyr Resinol, Actigenin or mixtures thereof were more effective in improving skin wrinkles, and in particular, the effect of Matyr Resinol was the most preferable for collagen synthesis.

Such matairesinol and / or actinigen can be obtained from the duct via the extraction, separation and purification methods commonly used in the field of the present invention, but may also be prepared through chemical synthesis methods commonly known in the art. have.

The present invention also provides a method for preparing a soft extract extract containing a large amount of the active ingredient for skin wrinkle improvement by treating the soft extract with acid or enzyme glycosidase. That is, by treating the glycosides contained in the soft-core extract with an acid or an enzyme, it is possible to increase the content of matairesinol and / or actinigen, which are non-glycosides excellent in skin wrinkle improvement.

It is preferable that the total content of matyrethinol and / or actinigen contained in the cosmetic composition is 0.001 to 10% by weight based on the total weight of the cosmetic composition. If the content of the active ingredient is less than 0.001% by weight, it is not preferable to achieve a sufficient wrinkle improvement effect of the original desired skin, if the content exceeds 10% by weight may cause problems in the stability of the cosmetic And, due to this there is a disadvantage that can not express a sufficient cosmetic effect when using or cosmetics.

The present invention is a cosmetic composition for improving skin wrinkles, characterized in that any one selected from the group consisting of cosmetics, essences, skins, lotions, creams, packs, foundations and makeup bases In addition, it provides a cosmetic composition for improving skin wrinkles, characterized in that the soft extract extract-containing cosmetic composition is used in the manufacture of a basic product cosmetics or color cosmetics. In addition, the cosmetic composition of the present invention can be applied in various forms such as liquid, cream, paste and solid, and can be prepared using a conventional cosmetic preparation method.

Hereinafter, the following examples and the like will be described in order to describe the present invention in more detail. However, embodiments according to the present invention can be modified in many different forms and the scope of the present invention should not be construed as being limited to the embodiments described below. Embodiments of the present invention are provided by way of example in order to facilitate a specific understanding of the present invention.

<Extraction Examples 1 to 4>

It surname is grown in North Gyeongsang castle forsythia (Forsythia viridissima Lindley was purchased directly from the Yeongcheon Herb Market.

Extraction, separation and purification of the four lignan compounds from Yeongyo were carried out as follows. 80% ethanol aqueous solution (2 L) was added to the coarsely pulverized dry bridge (300 g), followed by hot water extraction to obtain an ethanol crude extract (31.2 g). The solution was redissolved again with 80% ethanol aqueous solution (1 L), and then concentrated using an equal amount of a mixture of n-hexane, ether, and n-butanol to obtain an ether extract (1.4 g) and n-butanol extract (6.2 g). Respectively obtained.

Next, ether fractions were subjected to column chromatography with chloroform-ethanol (5: 1, v / v) mixed solvent using silica gel 60 (70-230 mesh, Merck, Germany) to obtain four fractions (Fr.1). , Fr.2, Fr.3, and Fr.4). Next, Fr. 2 was dissolved in ethanol, and 100% ethanol was used as a mobile phase to conduct a Sephadex LH-20 (Pharmacia, Sweden) column chromatography. Next, all separated fractions were measured for UV absorption spectra using a photo-diode array UV-VIS spectrophotometer (S1100, Scinco, Co., Korea), and then fractions where useful components were detected among the fractions having the same absorption spectrum (fraction 51 ~ 65, fractions 80-95) were pale yellow amorphous actigenin (17 mg; extraction example 1)) and matairesinol (14 mg; extraction example 2), respectively.

 Next, the n-butanol fraction was dissolved in chloroform-ethanol (3: 1, v / v) mixed solvent and subjected to silica gel column chromatography using the same solvent, and divided into seven fractions (Fr. 1, Fr. 2, Fr. 3, Fr. 4, Fr. 5, Fr. 6, Fr. 7). Next, Fr.2 and Fr.4 were dissolved in ethanol and Sephadex LH-20 column chromatography was performed with 100% ethanol as a mobile phase to actin (actiin, 450 mg; extraction Example 3) from Fr.2 and Fr.4. And matairesinoside (150 mg; extraction example 4) were each isolated purely. The yield of the soft-core extract according to each example is shown in Table 1 below.

Extraction Example Lignan Compound Yield One Actigenin 17 mg 2 Matai Resinol 14 mg 3 Actin 450 mg 4 Mata Leishinoside 150 mg

<Extraction Examples 5 to 6>

Separation and purification of the lignan compound (actininine, matyrethinol) by enzymatic treatment from Yeongyo was carried out as follows.

1 L of 80% ethanol aqueous solution was added to 100 g of dry soft bridge, followed by heating and extraction at 70-80 ° C. for 12 hours, followed by filtration. The extraction process was repeated twice to obtain ductile ethanol extract (300 g). The resultant was dissolved again in an aqueous 80% ethanol solution, n-hexane 300 mL was added and fractionated to remove fat and pigment from the supernatant, and the lower layer was concentrated to obtain a duct degreasing ethanol extract (18 g). This was again dissolved in 100 mL of 90% ethanol aqueous solution, filtered and concentrated to obtain a soft extract (15 g), of which 5 g was dissolved in 100 mL of 10% ethanol aqueous solution. Pectinex (Pectinex, Novozymes, Denmark) was added thereto as an enzyme, followed by hydrolysis while stirring at 50-60 ° C. for 1 hour, followed by cooling and equilibrating with 40% ethanol aqueous solution (Mistubishi Chem. Co., Japan) column was eluted sequentially with 40%, 60%, 80% ethanol aqueous solution and 100% ethanol, respectively. At this time, the part eluted with 80% ethanol aqueous solution was divided into two fractions and concentrated under reduced pressure to obtain purified powder I (0.13 g) and purified powder II (0.12 g), respectively. These powders were then separated with Sephadex LH-20 using 100% ethanol to give 106.83 mg of matyrethinol and 120.2 mg of actinigen, respectively. Next, 10 g of the remaining skim-bridged ethanol extract was repeated twice in the same manner as in the above procedure to obtain final matyrethinol (320.5 mg; extraction example 5) and actigenin (360.6 mg; extraction example 6), respectively. The yield of soft-bridge extracts according to each example is shown in Table 2 below.

Extraction Example Lignan Compound Yield 5 Actigenin 320.5 mg 6 Matai Resinol 360.6 mg

From the results of Tables 1 and 2, it can be seen that the nonglycoside that is an active phytoestrogen is obtained at a very high concentration by enzymatic treatment in the extraction process of the lignan compound.

Experimental Example 1. Antioxidant Effect Test

The free radical elimination test and the active oxygen elimination test were performed to examine the antioxidant effects of the anti-wrinkle effect assay test on the extracts prepared in each of the extraction examples 3 to 6 of the present invention.

Free radical scavenging test is a modification of the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299 ~ 303, 1993), which is a stable free radical DPPH (1,1-Diphenyl-2-picrylhydrazyl radical) Was used. Each extract sample obtained according to the extraction examples 3 to 6 in 1 mL of 0.2 mM DPPH solution was diluted with methanol in an appropriate concentration, mixed in 2 mL, and allowed to stand at room temperature for 10 minutes, and then absorbance was measured at 517 nm. The control group was measured in the same manner by adding methanol instead of the sample solution, and using the methanol instead of DPPH solution to obtain a color correction value for each of the extracted sample and the control.

By using the following equation 1 to calculate the free radical removal rate as a numerical value is shown in Table 3 below. In Table 3, IC ₅₀ is the concentration of the sample required to achieve 50% free radical scavenging ratio, which is a numerical value used in comparison with other component substances.

The active oxygen scavenging experiment is a modification of the method of Noro et al. (Chen. Pharm. Bull., 31, 3984 ~ 3987, 1983), and the active oxygen generation system by xanthine / xanthine oxidase enzyme reaction The change of absorbance due to oxidation of nitroblue tetrazolim (NBT) by active oxygen was measured.

2.4 mL of Na ₂ CO ₃ , 0.1 mL of xanthine, 0.1 mL of EDTA (ethylenediaminetetraacetic acid), 0.1 mL of BSA (bovine serum albumin), 0.1 mL of NBT, and 0.1 mL of sample were mixed well and allowed to stand at 25 ° C. for 10 minutes. After adding 0.1 mL of xanthine oxidase and reacting at 25 ° C. for 20 minutes, 6 mM CuCl ₂ was added to stop the reaction. Thereafter, the absorbance was measured at an optical wavelength of 560 nm. For the control group, purified water was added instead of the sample solution, and the same method was used. Purified water was used instead of the xanthine oxidase solution to obtain color correction values for each of the extracted sample and the control.

Using the following equation 2, the oxygen scavenging rate is calculated and shown as a numerical value. In Table 3 below, IC ₅₀ is a concentration of the sample required to achieve 50% of the active oxygen scavenging rate, which is a value used in comparison with other component substances, and the smaller the value, the higher the scavenging ratio.

Table 3 shows antioxidants of the duct extracts of Examples 3 to 6 and dibutylhydroxytoluene (BHT), a conventional synthetic antioxidant, and ascorbic acid (L-ascorbic acid) and tocopherol (dl-α-tocopherol), which are vitamins. The effects were compared.

sample Free radical scavenging, IC ₅₀ (ug / ml) Free radical activator, IC ₅₀ (mg / ml) Actin 60.06 4362 Mata Leishinoside 40.25 2956 Actigenin 5.75 1634 Matai Resinol 4.50 542 Dibutylhydroxytoluene 134.15 > 5000 Ascorbic Acid 2.90 - Tocopherol 18.30 (0.018 IU) 223 (0.22 IU)

As shown in Table 3, the extraction examples 3 to 6 showed a superior antioxidant effect of about 2-200 times compared to dibutyl hydroxyl toluene, known as a synthetic antioxidant. In addition, the active phytoestrogens nonglycosides, matyrethinol and actigenin, showed superior antioxidant effects compared to the glycosides actin and matyrethinoside.

As mentioned above, soft-leaved extracts can help delay the increase of skin wrinkles because they have free radicals and scavenging activity against free radical species that can accelerate skin wrinkle formation. However, since the antioxidant effect alone can not be seen to improve the wrinkles of the skin, the following evaluation experiments were conducted.

Experimental Example 2. Fibroblast Proliferation Effect Test

In order to confirm the proliferative effect of fibroblasts, the degree of proliferation of normal human fibroblasts was tested on the duct extracts prepared in each of the extraction examples 3 to 6 of the present invention.

Normal human fibroblasts (Detroit 551, KCLB 10110) used in this experimental example were used by the Korea Cell Line Bank. Cells were inoculated at a density of 1 × 10 ⁴ cells in DMEM (Dulbecco's Modified Eagle's Medium) with 10% bovine serum and incubated at 37% for 5% CO ₂ for one day. Samples were treated by diluting them in media containing no serum by concentration. After 1 day of incubation, the cell dye (MTT, 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) was added, followed by further incubation for 4 hours. The chromosome crystals were dissolved in dimethy sulfoxide (DMSO) and measured for absorbance at 540 nm for relative comparison. As a control, a culture medium without a sample was used. Using the following equation (3) to calculate the viability of the cell numerical value is shown in Table 4 below.

Extraction Example Cell proliferation effect 1 ug / mL 10 ug / mL 100 ug / mL Actin 100.1-102.9 102.1-102.9 102.8-105.4 Mata Leishinoside 100.1 ~ 103.1 101.5-103.4 103.2 ~ 104.7 Actigenin 101.1-103.6 101.6-104.3 122.1-132.0 Matai Resinol 102.0-107.1 98.9-101.9 114.3 ~ 131.1 Oleanolic acid 97.5-114.4 100.4 ~ 120.1 93.7-101.7 Retinyl palmitate 99.1 ~ 105.1 97.3 ~ 101.4 100.2-107.6

As in the results shown in Table 4, the duct extracts according to the present invention is equivalent to or higher than the oleanolic acid (oleanolic acid) or retinyl palmitate known to be effective in wrinkle improvement in cosmetics It showed a blast proliferation effect, and the cell proliferation effect on the fibroblasts having actinogen and matyrethinol extract of the non-glycosides of Example 5 and 6 was better than that of the actin and matairecinoside of the glycosides of Examples 3 and 4 . In addition, the cell proliferation ability of actigenin and matareresinol was high depending on the concentration, indicating that the cell proliferation effect of fibroblasts was excellent and that the non-glycoside concentration in the duct extract should be above a certain level when applied to the final cosmetic. Suggested.

Experimental Example 3. Collagen Synthesis Effect Test

The collagen synthesis performance of assaying the effect of skin wrinkle improvement on the duct extracts prepared in each of the extraction examples 3 to 6 of the present invention was measured by culturing fibroblasts. Human normal fibroblasts as in Experimental Example 2 were inoculated in a 24-well microplate at a concentration of 1 × 10 ⁵ cells / well, followed by CO ₂ at 37 ° C. and 5% concentration. The incubator was incubated for 24 hours. The soft-core extracts obtained in the extraction examples 3 to 6 were incubated for 24 hours in a serum-free DM medium (DMEM, Jeil Biotechservices) medium.

Collagen synthesis amount was performed using the enzyme linked immunosorbent assay (ELISA) as follows. Barries incubated for 24 hours were dispensed into 96-well microplates and coated overnight at 4 ° C. Washed three times with washing buffer (PBS; phosphate buffered saline) and blocking solution (5% skin milk) was added and blocked for 1 hour at 37 ℃. The blocking solution was discarded and washed three times with a washing buffer, and the primary antibody (rabbit anti-collagen type I) was diluted in PBS, added 100 ul, and reacted at 37 ° C. for 90 minutes. After washing three times with washing buffer, the secondary antibody (anti-rabbit IgG alkaline phosphatase conjugate) was diluted in PBS and added 100 ul each reaction was reacted for 90 minutes at 37 ℃. After washing three times with washing buffer, 100 ul of alkaline phosphatase substrate solution was added and developed at room temperature, and then the absorbance was measured at 405 nm using an ELISA reader (EL800, Bio-Tek Instruments, Inc.). . The measured value was expressed as collagen synthesis effect by Equation 4 below. At this time, the reaction absorbance of the cell culture without the sample was used as a control.

At this time, in order to determine the collagen synthesis effect of the duct extract, ascorbic acid was set as a comparison group, and the average value was obtained by performing three times each.

Table 5 shows the experimental results and the experimental results performed for the comparative group using different concentrations of the soft bridge extracts obtained in the extraction examples 3 to 6.

Test substance (ug / ml) Collagen Synthesis Effect (%) Actin 0 1 10 50 100 100.0 102.1 102.6 104.7 104.9 Mata Leishinoside 0 1 10 50 100 100.0 102.3 104.1 105.6 106.4 Actigenin 0 1 10 50 100 100.0 109.0 106.6 114.4 113.6 Matai Resinol 0 1 10 50 100 100.0 98.5 103.6 115.1 133.8 Ascorbic Acid 0 1 10 50 100 100.0 99.2 102.7 107.2 113.5

As shown in Table 5, the nonglycoside, actigenin, showed an effect similar to that of ascorbic acid, which is known to be an excellent collagen synthesizing effect. The effect was shown.

Experimental Example 4. Collagenase (MMP-1) Expression Inhibition Effect Test>

The inhibition of expression of collagenase (MMP-1), the expression of which was increased by UV-A irradiation of the soft-leaved extract obtained according to the extraction example 6 of the present invention, was carried out by the ELISA method used in Experimental Example 3.

Human normal fibroblasts as in Experimental Example 2 were inoculated into 24-well microplates (1 × 10 ⁵ cells / well) and incubated in a CO ₂ incubator at 37 ° C., 5% concentration for 24 hours. After irradiating UV-A in a range that does not affect the fibroblasts cultured using the UV chamber, the sample was incubated for 48 hours by replacing with the medium to which the sample was added, and then the medium was recovered and coated on a 96-well microplate. The blocking solution was treated for 1 hour to block sites where collagenase was not bound. After reacting the secondary antibody for about 90 minutes, the primary antibody was treated and reacted at 37 ° C. for 90 minutes, followed by color development by adding 100 ul of the substrate solution. The duct extract obtained in Extraction Example 6 was incubated in DMEM medium containing no serum added for each concentration for 24 hours. Absorbance was measured at 405 nm using an ELISA reader. The measured value represented the degree of inhibition of collagenase expression according to Equation 5 below. At this time, the reaction absorbance of the cell culture without the sample was used as a control.

At this time, in order to determine the inhibitory effect of collagenase expression of duct extract, oleanolic acid was set as a comparison group, and the average value was determined by performing three times each.

Test substance (ug / mL) Collagenase expression inhibitory effect (%) Matai Resinol 0 1 10 100 83.8 64.2 Oleanolic acid 0 1 10 100 76.1 69.3

As shown in Table 6, the extraction example 6 showed an inhibitory effect of collagenase synthesis to a degree similar to oleanolic acid, which is well known as a collagenase synthesis inhibitor.

<Formulation Example 1 and Comparative Example 1>

Component composition of the nutrient skin including the yeonchu extract according to the extraction example 6 was prepared as shown in Table 7. At this time, the unit of component content is weight%.

number ingredient Formulation Example 1 Formulation Comparative Example 1 One Purified water Remaining amount Remaining amount 2 Carbomer 0.05 0.05 3 Hydroxyethyl cellulose 0.05 0.05 4 Butylene Glycol 3.0 3.0 5 glycerin 2.0 2.0 6 Fiji-1500 0.5 0.5 7 ethanol 4.0 4.0 8 Polysorbate 80 0.2 0.2 9 Triethanolamine 0.05 0.05 10 antiseptic a very small amount a very small amount 11 Ducted Extract According to Extraction Example 6 0.2 －

Among the components identified by component number in Table 7, components 2 and 3 were first dispersed and dispersed in component 1 (purified water), and then components 4 to 6 were added, and components 8 to 10 dissolved in component 7 and fractionated. Component 11 dissolved in component 4 was added sequentially to prepare a mixture.

Comparative Example 1 for Formulation Example 1, except for component 11, the other components of the composition or manufacturing method was set up to proceed in the same manner.

<Formulation Example 2 and Comparative Example 2>

The ingredient composition of the nutrient lotion containing the duct extract according to the extraction example 6 was prepared as shown in Table 8. At this time, the unit of component content is weight%.

number ingredient Formulation Example 2 Formulation Comparative Example 2 One Cetearyl Alcohol 1.0 1.0 2 Glyceryl Stearate 0.7 0.7 3 Polysorbate 60 1.2 1.2 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyloctanoate 6.0 6.0 6 Squalane 4.0 4.0 7 Macadamia Nut Oil 3.0 3.0 8 Butylene Glycol 4.0 4.0 9 glycerin 4.0 4.0 10 Carbomer 0.15 0.15 11 Xanthan Gum 0.03 0.03 12 antiseptic a very small amount a very small amount 13 Purified water Remaining amount Remaining amount 14 Arginine 0.15 0.15 15 Ducted Extract According to Extraction Example 6 0.5 －

Among the components identified by each component number in Table 8 above, components 1 to 7 are first dissolved by heating at a temperature of 70 ° C., and then components 8 to 12 are dissolved and dispersed in component 13, and emulsified by heating to 70 ° C. Thereafter, the emulsified product was neutralized with component 14, cooled to a temperature of 56 ° C, and then, component 15 dissolved in the fractionated component 8 was added, stirred, and cooled at room temperature to prepare.

Comparative Example 2 with respect to Formulation Example 2, except for the ductile extract according to the extraction example 5 which is component 15, the other components of the composition or manufacturing method was set to proceed in the same manner.

<Formulation Example 3 and Comparative Example 3>

The composition of the nutrient essence containing the duct extract according to the extraction example 6 was prepared as shown in Table 9. At this time, the unit of component content is weight%.

number ingredient Formulation Example 3 Formulation Comparative Example 3 One Purified water Remaining amount Remaining amount 2 Carbomer 0.2 0.2 3 Hydroxyethyl cellulose 0.1 0.1 4 Butylene Glycol 8.0 8.0 5 glycerin 6.0 6.0 6 Sodium hyaluronate 150 15.0 7 ethanol 4.0 4.0 8 Polyoxyethylene Cured Castor Oil 0.1 0.1 9 Triethanolamine 0.2 0.2 10 antiseptic a very small amount a very small amount 11 Ducted Extract According to Extraction Example 6 5.0 -

Of the components distinguished by each component number in Table 9, components 2 and 3 were first dispersed and dispersed in component 1 (purified water), and then components 4 to 5 and 6 were added, and components 8 to 10 were dissolved in component 7. And component 11 dissolved in aliquot component 4 were added sequentially to prepare a mixture.

Comparative Example 3 with respect to Formulation Example 3, except for the soft-core extract according to the extraction example 5 of the component 11, the other components of the composition or manufacturing method was set to proceed in the same manner.

<Formulation Example 4 and Comparative Example 4>

The ingredient composition of the nutrient cream containing the duct bridge extract according to the extraction example 6 was prepared as shown in Table 10. At this time, the unit of component content is weight%.

number ingredient Formulation Example 4 Formulation Comparative Example 4 One Cetearyl Alcohol 1.5 1.5 2 Glyceryl Stearate 1.0 1.0 3 Polysorbate 60 1.2 1.2 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyloctanoate 6.0 6.0 6 Squalane 8.0 8.0 7 Apricot Kernel Oil 4.0 4.0 8 Dimethicone 1.0 1.0 9 Butylene Glycol 5.0 5.0 10 Grill serine 4.0 4.0 11 Magnesium Aluminum Silicate 0.2 0.2 12 Xanthan Gum 0.05 0.05 13 antiseptic a very small amount a very small amount 14 Purified water Remaining amount Remaining amount 15 Ducted Extract According to Extraction Example 6 1.0 －

On the other hand, among the components identified by each component number in Table 10 above, components 1 to 8 were first dissolved by heating at a temperature of 70 ° C, and then components 9 to 13 were dissolved and dispersed in component 14 and emulsified in heating to 70 ° C. . Thereafter, the emulsified product was cooled to a temperature of 56 ° C., and then component 15 dissolved in the fractionated component 9 was added thereto, stirred, and cooled to room temperature to prepare.

Comparative Example 4 with respect to Formulation Example 4, except for the soft-core extract according to extraction Example 5 which is component 15, the other components of the composition or the manufacturing method was set to proceed in the same manner.

<Formulation Example 5 and Comparative Example 5>

The composition of the nutritional pack containing the duct extract according to the extraction example 6 was prepared as shown in Table 11 below. At this time, the unit of component content is weight%.

number ingredient Formulation Example 5 Formulation Comparative Example 5 One Purified water Remaining amount Remaining amount 2 Butylene Glycol 3.0 3.0 3 glycerin 2.0 2.0 4 Polyvinyl alcohol 2.0 2.0 5 Polyvinylpyrrolidone 10.0 10.0 6 Cellulose gum 0.3 0.3 7 Mica 2.0 2.0 8 kaoline 2.0 2.0 9 ethanol 5.0 5.0 10 Polyoxyethylene Cured Castor Oil 0.3 0.3 11 antiseptic a very small amount a very small amount 12 Ducted Extract According to Extraction Example 6 2.0 -

Of the components identified by each component number in Table 11 above, components 2 and 3 are first added to component 1 (purified water), and then components 4 to 6 are added and heated to 70 ° C. while stirring and dispersing. The following components 7 to 8 are then added to disperse sufficiently and cooled to a temperature of 56 ° C. A substance prepared by dissolving components 10 to 11 in component 9 and component 12 dissolved in aliquoted component 2 in turn was stirred and cooled to room temperature.

Comparative Example 5 with respect to Formulation Example 5, except for the ductile extract according to the extraction example 5 which is component 12, the other components of the composition or manufacturing method was set to proceed in the same manner.

Experimental Example 5. Wrinkle improvement effect and skin irritation sensory test>

In order to evaluate the skin wrinkle improvement effect and skin irritation of the cosmetic composition for skin wrinkle improvement according to the present invention, a sensory test was conducted using the nutrition cream prepared in Formulation Example 4 and Comparative Example 4.

Specifically, in order to measure the antiwrinkle effect of the skin when the nourishing creams of Formulation Example 4 and Formulation Comparative Example 4 were applied to the skin, the left side of the face of the nourishing cream of Formulation Example 4 Test group), the right side of the face was allowed to continuously use the nutrition cream (control) of the formulation Comparative Example 4 once a day for 12 weeks.

For the anti-wrinkle effect item of the skin in the sensory test, the anti-wrinkle effect of the nutritional cream of the formulation example 4 was relatively evaluated based on the nutritional cream of the formulation of Comparative Example 4. Phenomena such as stinging, tingling and erythema were evaluated. Evaluation was performed based on the five-point standard of very good (5 points), excellent (4 points), moderate (3 points), bad (2 points), very bad (1 point), the results are shown in Table 12 Indicated. In Table 12, the skin irritation indicates the degree of no skin irritation.

number Skin irritation Wrinkle improvement effect Formulation Example 4 Formulation Comparative Example 4 One 4 5 5 2 5 5 5 3 5 4 5 4 3 4 4 5 4 4 5 6 5 5 5 7 4 4 4 8 4 4 4 9 5 5 5 10 4 4 4 11 5 5 5 12 5 5 5 13 3 4 4 14 4 4 4 15 5 4 4 16 4 4 4 17 5 5 5 18 5 5 5 19 5 5 5 20 4 4 4 Average 4.40 4.45 4.55

As shown in Table 12 below, the skin irritation evaluation score for the cosmetic composition of Formulation Example 4 according to the present invention was very good as 4.40, and the skin irritation degree was low, as in Comparative Formulation Example 4. Could confirm.

In addition, it can be seen that the relative improvement of the wrinkles of the skin of the cosmetic composition of Formulation Example 4 compared to Formulation Comparative Example 4 was 4.55, an excellent degree of improvement.

Experimental Example 6. Skin Safety Experiment

In order to determine the occurrence of irritation or allergic reaction through the skin safety, that is, the skin reaction for the extract Example 4 and the formulation of Example 4 according to the extraction Example 6 in accordance with the Korea Food and Drug Administration standard skin safety test method The test was conducted.

Thirty healthy men and women aged twenty to fifty years were selected as subjects. The patch test was carried out on the inner side of the subject's upper arm and the patch was removed after 48 hours. The first reading was performed after about 60 minutes of stability, and the second reading was taken after 96 hours of patch application. Criteria are as shown in Table 13 below.

reaction weight Criteria of Judgment - 0 No response ± 0.5 Faint erythema + One Boundary but weak erythema, edema and papules ++ 2 Pronounced erythema, papules and blisters

The skin readiness of the sample was calculated using Equation 6 below for 48 hours and 96 hours after patch application.

The skin irritation degree of the sample showing the positive response calculated based on the test result of the patch test is shown in Table 14 below.

sample 48 hours 96 hours Responsiveness (%) + ++ + ++ 48 hours 96 hours Average Extraction Example 6 (2% in 1,3-butylene glycol) - - One - 0.00 0.56 0.28 Formulation Example 4 - - 2 - 0.00 1.11 0.56

As shown in Table 14, each of Extraction Example 6 and Formulation Example 4 according to the skin reactivity determination criteria according to Table 15 in the human scaffold test was found to be good results for use on sensitive skin without irritation. Therefore, the soft-leaved extract according to the extraction examples appeared to be a safe ingredient with no natural side effects as a natural product.

Average responsiveness of the skin Criteria 0.0 to 0.9 Non-irritating 1.0 to 2.9 Light stimulation 3.0 to 4.9 Heavy stimulation 5.0 or more Strong stimulation

As described above, the present invention provides a cosmetic composition containing matyresinol and / or actinigen as an active ingredient. The cosmetic composition according to the present invention has no skin side effects as a natural product, and has excellent anti-oxidation effect, cell proliferation effect, collagen synthesis effect, and expression inhibitory effect of collagenase, an enzyme that decomposes collagen, and wrinkles of skin. Very useful for improving

Claims (5)

A cosmetic composition for improving skin wrinkles, which comprises Forsythia Fruit extract. A cosmetic composition for improving skin wrinkles, comprising matairesinol, actinigen, or a mixture thereof. The cosmetic composition for improving skin wrinkles according to claim 2, wherein the cosmetic composition contains matyrethinol. The cosmetic composition for improving skin wrinkles according to claim 2, wherein the total content of the matyrethinol, actinigen or a mixture thereof is 0.001 to 10% by weight based on the total weight of the cosmetic composition. According to claim 1 or 2, wherein the cosmetic composition is any one selected from the group consisting of cosmetics, essences, skins, lotions, creams, packs, foundations and makeup bases. Cosmetic composition for improving skin wrinkles.