KR20010096669A - cosmetic composition containing plant extract complex - Google Patents

Abstract

PURPOSE: A cosmetic composition containing an extract obtained from a vegetable mixture of Portulaca oleracea L., rosemary, Scutellaldose baicalensis, Angelica archangelica, pine mushroom, arbutin, Panax ginseng and vegetable placenta is provided, which has skin whitening and cell proliferation effects. CONSTITUTION: This cosmetic composition contains 0.01 to 10% by weight of a plant extract obtained by adding one or more solvents selected from the group consisting of distilled water, ethanol, methanol, butanol, acetone, ethyl acetate, hexane, propanol, hydrous butylene glycol and hydrous propylene glycol to a mixture of 15 to 25% by weight of Portulaca oleracea L., 20 to 25% by weight of rosemary, 5 to 15% by weight of Scutellaldose baicalensis, 15 to 25% by weight of Angelica archangelica, 5 to 20% by weight of pine mushroom, 1 to 5% by weight of arbutin, 5 to 15% by weight of Panax ginseng and 5 to 15% by weight of vegetable placenta and extracting at 50 to 95deg.C for 4 to 20 hr.

Description

Cosmetic composition containing a mixed plant extract having a whitening and cell proliferation effect {cosmetic composition containing plant extract complex}

The present invention relates to a cosmetic composition exhibiting a whitening and cell proliferation effect containing an extract obtained from a botanical mixture of machi, rosemary, golden, fresh vine, matsutake, arbutin, red ginseng, and vegetable placenta.

Skin is a very important tissue that has direct contact with the external environment, protects the human body and has biochemical and physical functions. This tissue is largely divided into three parts: the epidermis, the dermis, and the hypodermis. Melanin is a black pigment produced in the epidermis and is produced in cells called melanocytes. Melanin absorbs the light energy of sunlight UV and protects cells deeper from UV damage. However, abnormally low production of melanin causes skin lesions such as vitiligo and, on the contrary, excessive synthesis of melanin by UV light not only damages the skin but also forms spots and freckles. It can also lead to skin cancer from these lesions.

Skin is also a tissue with the ability to heal functional and structural damage. However, aging and various diseases lower the proliferation and regeneration of these cells, thereby losing skin flexibility and elasticity. Therefore, cell proliferation ability of skin tissue cells is an important factor in skin health. However, if the cells are physiologically inactivated due to various contaminants and stress, and the cells are not regenerated and proliferated properly, the skin may be damaged and cause rapid skin aging. Therefore, if the cell formation ability by inducing active cell division of these cells can be expected to be healthy skin as well as the effect of inhibiting skin aging, such as increased skin elasticity, wrinkle suppression.

Animal extracts or various synthetic materials have been developed for the whitening and anti-ageing, but most of them have a problem that many of them have problems or do not obtain sufficient effects on stability and safety.

Accordingly, it is an object of the present invention to provide a vegetable natural extract having no problems in stability and safety with excellent whitening and cell proliferation effects.

Another object of the present invention is to provide a cosmetic composition comprising an extract having such efficacy.

1 is a graph showing the skin whitening effect of the cosmetic composition of the present invention.

Figure 2 is a graph showing the skin elasticity improving effect of the cosmetic composition of the present invention.

In view of the above, the present inventors searched for substances that are effective in whitening and cell proliferation among various natural products in nature in order to find the fundamental substances having whitening and cell proliferation potency, and as a result, machi, rosemary, golden, fresh herb, pine, arbutin, It was found that the mixed plant extract of red ginseng and plant placenta showed excellent whitening and cell proliferation effect.

In the present invention, as a result of searching for the whitening and cell proliferation effect of the mixed plant extract obtained by extracting these eight natural products at a constant mixing ratio, it was found that the effect is superior to the respective extracts and the test results solved the stability and safety problems at the same time.

In addition, it was found that even in a test result of directly applying the mixed plant extract in a cosmetic composition to a certain concentration, the skin whitening and cell proliferation effect was also observed.

The present invention provides a cosmetic composition containing a mixed plant extract of machi, rosemary, golden, fresh vinegar, pine, arbutin, red ginseng, vegetable placenta, which are effective in preventing elasticity of melanin and enhancing elasticity by cell proliferation.

The extract is added to cosmetics, that is, softening water (skin), nourishing lotion (lotion), nourishing cream, massage cream, essence, pack, and the like.

The present invention will be described in more detail as follows.

Machi, rosemary, golden, fresh vinegar, matsutake, arbutin, red ginseng, vegetable placenta are dried 15-15%, 20-25% of rosemary, 5-15% of golden, 15-25 of fresh vinegar, respectively. %, Clusters 5-20%, arbutin 1-5%, red ginseng 5-15%, vegetable placenta, 5-15% dry weight ratio, distilled water, ethanol, methanol, butanol, acetone, 10-20 times by volume of at least one solvent selected from the group consisting of ethyl acetate, hexane, propanol, hydrous butylene glycol, hydrous propylene glycol is added. Extraction method is a method of extracting by heating for 4-20 hours at 50-95 ℃, or extracting the active ingredient at 5-37 ℃ 1-15 days in a state that the cooling condenser is installed to prevent the solvent from evaporating. Can be used.

The extracted plant extracts, such as machi, rosemary, golden, fresh vinegar, pine, arbutin, red ginseng, and vegetable placenta, are concentrated under reduced pressure while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser. It is added to the cosmetic in an amount of -10%, preferably 0.01-5%.

Hereinafter, the present invention will be described in more detail using examples.

The following Examples and Experimental Examples are for explaining the contents of the present invention, but the contents of the present invention are not limited thereto.

Extract Preparation Example 1.

Completely dried, 200 g of gusset, 200 g of rosemary, 100 g of golden silver, 200 g of fresh vinegar, 100 g of clusters, 50 g of arbutin, 100 g of red ginseng, 50 g of vegetable placenta, were put into 10 kg of distilled water and boiled for 3 hours in an extractor with a cooling condenser. After filtration with a 400 mesh filter cloth and cooled to room temperature, the mixture was left to age for 7 days at 5-15 ° C., and then filtered with Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure to 80 in a distillation apparatus equipped with a cooling condenser to obtain 34 g (dry weight).

Extract Preparation Example 2

Completely dried, 200 g machi, 200 g rosemary, 100 g of golden silver, 200 g of fresh vinegar, 150 g of vinegar, 50 g of arbutin, 50 g of red ginseng, 50 g of vegetable placenta were put into 10 kg of ethanol and boiled for 3 hours in an extractor with a cooling condenser. After filtration with a 400 mesh filter cloth and cooled to room temperature, the mixture was left to age for 7 days at 5-15 ° C., and then filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure to 80 in a distillation apparatus equipped with a cooling condenser to obtain 35 g (dry weight).

Extract Preparation Example 3.

1 g of dried dried machi, 150 g of rosemary, 250 g of golden silver, 150 g of fresh vinegar, 150 g of vinegar, 50 g of arbutin, 50 g of red ginseng, and 50 g of vegetable placenta were put into 10 kg of ethanol and extracted by boiling for 3 hours in an extractor equipped with a cooling condenser. After filtration with a 400 mesh filter cloth and cooled to room temperature, the mixture was left to age for 7 days at 5-15 ° C., and then filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure to 80 in a distillation apparatus equipped with a cooling condenser to obtain 37 g (dry weight).

Extract Preparation Example 4.

1 kg of dried dried machi, 200 g of rosemary, 200 g of golden, 100 g of fresh vinegar, 150 g of vinegar, 50 g of arbutin, 50 g of red ginseng, and 50 g of vegetable placenta were put into 10 kg of butanol and extracted by boiling in an extractor with a cooling condenser for 3 hours. After filtration through a mesh filter cloth and cooled to room temperature, the mixture was left to mature at 5-15 ° C. for 7 days, and then filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure to 80 in a distillation apparatus equipped with a cooling condenser to obtain 29 g (dry weight).

Extract Preparation Example 5.

After drying completely, dried 1 g machi, 200 g of rosemary, 250 g of golden, 150 g of fresh vinegar, 150 g of vinegar, 50 g of arbutin, 50 g of red ginseng, and 50 g of vegetable placenta were put into 10 kg of acetone and extracted by boiling for 3 hours in an extractor with a cooling condenser. After filtration through a mesh filter cloth and cooled to room temperature, the mixture was left to mature at 5-15 ° C. for 7 days, and then filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure to 80 in a distillation apparatus equipped with a cooling condenser to obtain 32 g (dry weight).

Extract Preparation Example 6.

150 g of dried machi, 100 g of rosemary, 200 g of golden silver, 200 g of fresh vinegar, 150 g of vinegar, 50 g of arbutin, 100 g of red ginseng, and 50 g of vegetable placenta were added to 10 kg of ethyl acetate and boiled for 3 hours in an extractor with a cooling condenser. After filtration with a 400 mesh filter cloth and cooled to room temperature, the mixture was left to age for 7 days at 5-15 ° C., and then filtered with Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure to 80 in a distillation apparatus equipped with a cooling condenser to obtain 33 g (dry weight).

Extract Preparation Example 7.

Completely dried, 200 g of machi, 200 g of rosemary, 100 g of golden silver, 200 g of fresh vinegar, 150 g of vinegar, 50 g of arbutin, 50 g of red ginseng, and 50 g of vegetable placenta were added to 10 kg of hexane and boiled for 3 hours in an extractor with a cooling condenser. After filtration with a 400 mesh filter cloth and cooled to room temperature, the mixture was left to age for 7 days at 5-15 ° C., and then filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure to 80 in a distillation apparatus equipped with a cooling condenser to obtain 30 g (dry weight).

Extract Preparation Example 8.

Completely dried, 200 g machi, 200 g of rosemary, 100 g of golden silver, 200 g of fresh vinegar, 150 g of vinegar, 50 g of arbutin, 50 g of red ginseng, and 50 g of vegetable placenta were added to 10 kg of propanol and boiled for 3 hours in an extractor with a cooling condenser. After filtration with a 400 mesh filter cloth and cooled to room temperature, the mixture was left to age for 7 days at 5-15 ° C., and then filtered through Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure to 80 in a distillation apparatus equipped with a cooling condenser to obtain 32 g (dry weight).

Extract Preparation Example 9.

1 kg of dried dried machi, 200 g of rosemary, 200 g of golden silver, 200 g of fresh vinegar, 150 g of vinegar, 50 g of arbutin, 50 g of red ginseng, and 50 g of vegetable placenta were placed in 10 kg of hydrous butylene glycol and 3 hours in an extractor with cooling condenser. Boiled and extracted, filtered with a 400 mesh filter cloth, cooled to room temperature and left to mature for 7 days at 5-15 ℃ and then filtered with Whatman No. 2 filter paper. This mixed plant extract was concentrated under reduced pressure to 80 in a distillation apparatus equipped with a cooling condenser to obtain 39 g (dry weight).

Extract Preparation Example 10.

1 g of dried dried machi, 200 g of rosemary, 200 g of golden silver, 200 g of fresh vinegar, 150 g of vinegar, 50 g of arbutin, 50 g of red ginseng, and 50 g of vegetable placenta were put into 10 kg of butylene glycol and boiled in an extractor with a cooling condenser for 3 hours. After extraction, the filtrate was filtered through a 400 mesh filter cloth, cooled to room temperature, left to mature at 5-15 ° C. for 7 days, and then filtered using Whatman No. 2 filter paper. The mixed plant extract was concentrated under reduced pressure to 80 in a distillation apparatus equipped with a cooling condenser to obtain 36 g (dry weight).

Experimental Example 1.

Tyrosinase inhibitory effect was measured for the preparation examples of the mixed plant extracts of Machi, Rosemary, Golden, Sincho, Matsutake, Arbutin, Red Ginseng, and Plant Placenta.

1) Experiment Method

To 1.0 ml of L-tyrosine (0.3 mg / ml), add 1.0 ml of potassium phosphate buffer solution (pH 6.8, 0.1M) and 0.9 ml of mixed plant extract at different concentrations at room temperature and hold for 10 to 20 minutes at 37. Then add 0.1 ml of tyrosinase (1250 units / ml) and hold for 10 minutes. The reaction was terminated under freezing conditions consisting of ice water after taking out within 10 minutes ± 5 seconds. At this time, the control group was added 30% butylene glycol instead of the mixed plant extract of each concentration. Finally, the absorbance is examined at 475 nm. The tyrosinase inhibitory effect of each plant extract at each concentration is determined by the following formula.

% Tyrosinase inhibition =

[1-(enzyme activity of control plant extract at each concentration / enzyme activity of control)] X 100

Table 1. Measurement of Whitening Effect on Mixed Plant Extracts

Example Final concentration of the mixed plant extract contained in the reaction solution (㎍ / ml) Tyrosinase inhibition rate (%) Example 1 200100 8548 Example 2 200100 8852 Example 3 200100 9258 Example 4 200100 8841 Example 5 200100 8952 Example 6 200100 7870 Example 7 200100 7238 Example 8 200100 8252 Example 9 200100 9670 Example 10 200100 9058

Experimental Example 2.

The whitening effect of melanoma B16 was measured against the mixed plant extracts of Machi, Rosemary, Golden, Sincho, Matsutake, Arbutin, Red Ginseng, and Plant Placenta.

1) Experimental method.

Melanin production inhibition function was assayed at the cellular level through melanocyte culture. B16 melanoma cells are incubated for 10 days in 10% bovine melanoma cells in the presence of 5% carbon dioxide until the 1.0 X 108 cells / T25 flask. Then mix the plant extracts by concentration and incubate for 2 days in the presence of 5% carbon dioxide. Then trypsin is added to collect the cells and observe the degree of blackening of the cells. Melanin was quantified by measuring the absorbance at 475 nm, taking the supernatant of the T25 flask discharged from melanin, and the number of cells was mixed under the ratio of collected cells: tryptophan blue = 1: 9 and observed under a microscope. Count the amount of melanin produced per unit cell to show the inhibitory effect in%.

Table 2. Measurement of Whitening Effect on Mixed Plant Extracts

Example Final concentration of the mixed plant extract contained in the reaction solution (㎍ / ml) Melanogenesis inhibition rate (%) Example 1 5010 8039 Example 2 5010 7528 Example 3 5010 7734 Example 4 5010 7319 Example 5 5010 6925 Example 6 5010 5416 Example 7 5010 4622 Example 8 5010 6530 Example 9 5010 8537 Example 10 5010 7930

Experimental Example 3. Experiment of cell proliferation effect on mixed plant extract

The effect of cell proliferation was measured for the examples of Machi, Rosemary, Golden, Sincho, Matsutake, Arbutin, Red Ginseng, and Plant Placenta mixed extract.

Experiment method

100 μl of 5% young calf serum (FBS) solution was added to a 96 microwell plate, and then the sample was dispersed in a predetermined amount in DMEM medium containing 2% FBS, diluted, and added again by 100 μl. Using a hemocytometer, fibroblasts obtained from neonatal foreskin tissue were dispersed in 100 μl medium per well and cultured for 7 days. After incubation, the supernatant was removed and washed again by adding 200 μl of 5% PBS (phosphate buffered saline), and 150 μl of DMSO was added per well, followed by shaking for 10 minutes to measure absorbance at 570 nm. At this time, the experiments were conducted in the same manner without adding sprout mixture extract as a control, and the effect of cell proliferation was compared from the difference in relative absorbance.

Table 3. Cell Proliferation Effects on Mixed Plant Extracts

Example Final concentration of the sample contained in the reaction solution (㎍ / ml) Cell proliferation effect (%) Example 1 100 107 Example 2 100 130 Example 3 100 121 Example 4 100 113 Example 5 100 109 Example 6 100 111 Example 7 100 119 Example 8 100 116 Example 9 100 111 Example 10 100 105

Prescription Example 1.

A prescription example of a lotion (skin lotion) in cosmetics containing the extract of the plant mixture of the present invention is as follows. The extract used herein is that of Example 9.

number Raw material Prescription Example 1 Comparative Example 1 One Mixed plant extracts 4.0 - 2 glycerin 3.0 3.0 3 Butylene Glycol 2.0 2.0 4 Propylene glycol 2.0 2.0 5 Polyoxyethylene (40) Cured Castor Oil 1.0 1.0 6 ethanol 10.0 10.0 7 Citric acid 0.1 0.1 8 Sodium citrate 0.2 0.2 9 antiseptic a very small amount a very small amount 10 Pigment a very small amount a very small amount 11 Spices a very small amount a very small amount 12 Purified water a very small amount Remaining amount

<Manufacturing method>

Formulation Example 1: 2,3,4,7,8,9 in 12 are added in order to dissolve it, and then dissolved by heating 5 times to dissolve, and 11 is added to 12 for stirring. . Finally, 1, 6, 10 times are added and fully stirred before aging.

Comparative Example 1: 2, 3, 4, 7, 8, and 9 were added sequentially in order to dissolve by stirring, and 5 times were heated to 60 so as to dissolve, followed by 11 inputs, followed by stirring. . Finally, 6, 10 times are added and fully stirred before aging.

Prescription Example 2.

Prescription examples of nutritional lotions in the cosmetics of the plant mixture of the present invention are as follows. The extract used herein is that of Example 9.

number Raw material Prescription Example 1 Comparative Example 1 One Mixed plant extracts 6.0 - 2 Cetearyl Alcohol 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 4 Ceramide 0.7 0.7 5 Ceteares-4 1.2 1.2 6 cholesterol 1.5 1.5 7 Dicetylphosphate 0.4 0.4 8 glycerin 5.0 5.0 9 Sunflower oil 10.0 10.0 10 Carbomer 0.2 0.2 11 Xanthan Gum 0.3 0.3 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 2: After mixing and stirring the raw materials 8,10,11,12,14, the mixture was heated to 80-85 ° C and introduced into the manufacturing part, and then the emulsifier was activated and 2,3,4,5,6,7,9 Was heated to 80 to 85 ° C. and then emulsified. After emulsification, the mixture was cooled to 45 ° C. while stirring using a stirrer, and 13 times were added thereto. After cooling to 25 ℃ it was aged.

Comparative Example 2: After mixing and stirring the raw materials 8,10,11,12,14, the mixture was heated to 80 ~ 85 ° C. and introduced into the manufacturing unit, and then the emulsifier was activated and 2,3,4,5,6,7,9 It heated and heated to 80-85 degreeC, and emulsified. After emulsification, the mixture was cooled to 45 ° C. while stirring using a stirrer, and 13 times were added thereto, cooled to 25 ° C., and aged.

Prescription Example 3.

Prescription example of nutritional cream in the cosmetic containing the extract of the plant mixture of the present invention is as follows. The extract used herein is that of Example 9.

number Raw material Prescription Example 1 Comparative Example 1 One Mixed plant extracts 10.0 - 2 Cetearyl Alcohol 4.0 4.0 3 Glyceryl Monostearate 3.0 3.0 4 Ceramide 0.7 0.7 5 Polysorbate 80 2.0 2.0 6 cholesterol 3.0 3.0 7 Dicetylphosphate 0.4 0.4 8 glycerin 5.0 5.0 9 Sunflower oil 22.0 22.0 10 Carbomer 0.5 0.5 11 Xanthan Gum 0.5 0.5 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount Remaining amount

<Manufacturing method>

Formulation Example 3 While mixing and stirring the raw materials 8,10,11,12,14 and heating to 80 ~ 85 ° C. to the manufacturing part, the emulsifier is activated and 2,3,4,5,6,7,9 It heated and heated to 80-85 degreeC, and emulsified. After emulsification, the mixture was cooled to 45 ° C. while stirring using a stirrer, and 13 times were added thereto. After cooling to 25 ℃ it was aged.

Comparative Example 3: After mixing and stirring the raw materials 8,10,11,12,14 to 80 ~ 85 ° C. and putting them into the manufacturing part, the emulsifier was activated and 2,3,4,5,6,7,9 It heated and heated to 80-85 degreeC, and emulsified. After emulsification, the mixture was cooled to 45 ° C. while stirring using a stirrer, and 13 times were added thereto, cooled to 25 ° C., and aged.

Prescription Example 4

A prescription example of a massage cream in cosmetics containing the extract of the plant mixture of the present invention is as follows. The extract used herein is that of Example 9

number Raw material Example 2 Comparative Example 2 One Mixed plant extracts 5.0 - 2 Beeswax 4.0 4.0 3 Polysorbate 60 1.0 1.0 4 Sorbitan sesquioleate 0.8 0.8 5 Liquid paraffin 40.0 40.0 6 Squalane 5.0 5.0 7 Caprylic / Capric Triglycerides 4.0 4.0 8 Carbomer 0.2 0.2 9 glycerin 5.0 5.0 10 Butylene glycol 3.0 3.0 11 Propylene glycol 3.0 3.0 12 Triethanolamine 0.2 0.2 13 antiseptic a very small amount a very small amount 14 Pigment a very small amount a very small amount 15 Spices a very small amount a very small amount 16 Purified water Remaining amount Remaining amount

<Manufacturing method>

Prescription example 4: Mixing and stirring 8,9,10,11,13,16 and heating to 80 ~ 85 ° C to put into the manufacturing part, then actuated emulsifier and 2,3,4,5,6,7,12 It heated and heated to 80-85 degreeC, and emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and 14 times were added thereto, then cooled to 45 ° C. and 15 times were added thereto. After cooling to 25 ℃ it was aged.

Comparative Example 4: 8,9,10,11,13,16 and stirred at 80-85 ° C. while mixing and stirring to put into the manufacturing unit, and then actuated emulsifier and 2,3,4,5,6,7,12 It heated and heated to 80-85 degreeC, and emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and then 14 times added, cooled to 45 ° C., 15 times added, cooled to 25 ° C., and aged.

Prescription Example 5

Prescription examples of nutritional essences in cosmetics containing the extract of the plant mixture of the present invention are as follows. The extract used herein is that of Example 9

number Raw material Example 2 Comparative Example 2 One Mixed plant extracts 10.0 - 2 Cetearyl Alcohol 1.70 1.70 3 Glycerin Monostearate 1.50 1.50 6 Vitamin f 0.7 0.7 5 Poly Sorbate 80 1.2 1.2 4 cholesterol 1.5 1.5 7 Dicetylphosphate 0.4 0.4 8 glycerin 5.0 5.0 9 Sunflower oil 15.0 15.0 10 Carbomer 0.2 0.2 11 Xanthan Gum 0.2 0.2 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Remaining amount a very small amount

<Manufacturing method>

Formulation Example 5: Mixing and stirring the raw materials 8,10,11,12,14 and heating to 80 ~ 85 ° C. and putting them into the manufacturing part, then actuating emulsifier and 2,3,4,5,6,7,9 It heated and heated to 80-85 degreeC, and emulsified. After emulsification, the mixture was cooled to 45 ° C. while stirring using a stirrer, and 13 times were added thereto. After cooling to 25 ℃ it was aged.

Comparative Example 5: The raw materials 8,10,11,12,14 were mixed and stirred and heated to 80-85 ° C. and introduced into the manufacturing unit, followed by the action of an emulsifier and 2,3,4,5,6,7,9 It heated and heated to 80-85 degreeC, and emulsified. After emulsification, the mixture was cooled to 45 ° C. while stirring using a stirrer, and 13 times were added thereto, cooled to 25 ° C., and aged.

Experiment to confirm whitening effect on skin

Experimental Example 1. Skin erythema & melanin index measurement experiment

In order to examine the effect of the mixed plant extract on the whitening effect when the nutrient lotions of Example 2 and Comparative Example 2 were applied to the skin, 20 women were selected as the 20-55 year old female layer. 3MED (Minimum Ederma Dose) ultraviolet rays were exposed to the left and right arms with a mexameter, and the nutrition lotion of Example 2 was applied to the inside of the left arm, and the nutrition lotion of Comparative Example 2 was applied to the inside of the right arm. After applying twice a day in the morning and evening, erythema index and melanin index were measured using Mexameter after 1 day, 3 days, 5 days, 1 week, 2 weeks, 3 weeks and 4 weeks. The skin whitening effect measured by mexameter is to measure the amount of erythema and melanin production. The smaller the value, the better the whitening effect. The measurement results are shown in Table 1 and FIG. 1.

Table 1

apply Improvement Number of people Melanin content (%) Erythema amount (%) Example 2 It is effective 11 2 3 is average 6 7 5 There are some changes 3 12 9 No change - - - Comparative Example 2 It is effective - - - is average 4 8 6 There are some changes 4 11 11 No change 12 17 15

It can be seen that the skin whitening effect is superior to Comparative Example 2 prepared without adding the cosmetic of Example 2 prepared by adding the mixed plant extract as shown in the investigation result.

Test Example 2 Test for Increased Elasticity of Skin

In order to measure the effect of increasing the elasticity of the skin when the nourishing creams of Formulation Example 3 and Comparative Formulation Example 3 are applied to the skin, 20 to 55 years old women face the normal, oily, dry and combination skin owners On the left part, the nutrition cream of Formulation Example 3 was applied, and on the right part of the face, the nutrition cream of Comparative Formulation Example 3 was applied twice a day in the morning and evening, and then the skin was obtained after 1, 2, 3 and 4 weeks. The elasticity of the skin was measured using a elasticity analyzer (Ballistometer). The measurement results are shown in the following Table 2 and FIG.

TABLE 2

Cosmetic formulation of Formulation Example 3 Cosmetics of Comparative Formulation Example 3 Remarks T ₀ T ₁ T ₂ T ₃ T ₄ T ₀ T ₁ T ₂ T ₃ T ₄ Elasticity Spring water 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 amplitude first 1.8 4.3 5.6 6.7 7.5 1.7 3.3 4.0 4.2 4.5 second 1.5 3.1 4.2 5.3 6.1 1.6 2.2 2.5 3.0 3.5 Third time 0.0 2.2 2.6 4.4 5.6 0.0 0.0 1.9 2.2 2.7 Fourth 0.0 0.0 1.5 3.6 4.3 0.0 0.0 0.0 0.0 0.3 Average 0.825 2.4 3.475 5.00 5.85 0.825 1.375 2.1 2.35 2.75

(Note) T ₀ ; Before use

T ₁ ; After 1 week

T ₂ ; 2 weeks

T ₃ ; After 3 weeks

T ₄ ; After 4 weeks

As can be seen from the above results, the skin by the effect of improving the skin condition of the mixed plant extract compared to the nutritional cream of Comparative Example 3 prepared without the nutrition cream of Formulation Example 3 containing the mixed plant extract according to the present invention It can be seen that there is an elastic effect.

The mixed plant extract of the present invention has tyrosinase inhibitory activity and has an action of inhibiting melanin production. It also has a cell proliferation effect. As can be seen in the above embodiment, when the cosmetic composition containing the mixed plant extract of the present invention is applied to the skin, the whitening effect and skin elasticity improving effect is excellent.

Claims (7)

One selected from the group consisting of distilled water, ethanol, methanol, butanol, acetone, ethyl acetate, hexane, propanol, hydrous butylene glycol, and hydrous propylene glycol in a mixture of machi, rosemary, golden, fresh vine, cluster, arbutin, red ginseng, and vegetable placenta 10-20 times the volume of the solvent was added at 50-95 ℃, extracted by heating for 4-20 hours or extracted by deposition for 1-15 days at 5-37 ℃ plant extract having a cell proliferation effect . According to claim 1, 15-25% of machi, 20-25% of rosemary, 5-15% of gold, 15-25% of fresh vinegar, 5-20% of clusters, 1-5% of arbutin, red ginseng Plant extract, characterized in that 5 to 15%, plant placenta is mixed in a dry weight ratio of 5 to 15%. A cosmetic composition having a whitening and cell proliferating effect, comprising plant extracts extracted from machi, rosemary, golden, fresh vinegar, pine, arbutin, red ginseng, and plant placenta mixture. According to claim 3, 15-25% of machi, 20-25% of rosemary, 5-15% of gold, 15-25% of fresh vinegar, 5-20% of matsutake, 1-5% of arbutin, red ginseng A cosmetic composition having a whitening and cell proliferation effect, characterized in that 5 to 15%, plant placenta is mixed in a dry weight ratio of 5 to 15%. The method of claim 3, wherein the extract is a plant extract, such as distilled water, ethanol, methanol, butanol, acetone, ethyl acetate, hexane, propanol, hydrous butylene glycol At least one solvent selected from the group consisting of hydrous propylene glycol is added by adding a volume of 10-20 times and extracted by heating at 50-95 ° C. for 4-20 hours, or by depositing at 5-37 ° C. for 1-15 days. Cosmetic composition having a whitening and cell proliferation effect. 4. The cosmetic composition of claim 3, wherein the mixed plant extract is contained in an amount of 0.01 to 10% (w / v) as dry weight thereof. The cosmetic composition according to any one of claims 3 to 6, wherein the cosmetic composition has a whitening and cell proliferation effect having a formulation such as lotion (skin lotion), nutrition lotion, nutrition cream, massage cream, nutrition essence, and the like.