KR20000038844A - Whitening cosmetic base composition comprising mung bean extract - Google Patents

Whitening cosmetic base composition comprising mung bean extract Download PDF

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Publication number
KR20000038844A
KR20000038844A KR1019980053972A KR19980053972A KR20000038844A KR 20000038844 A KR20000038844 A KR 20000038844A KR 1019980053972 A KR1019980053972 A KR 1019980053972A KR 19980053972 A KR19980053972 A KR 19980053972A KR 20000038844 A KR20000038844 A KR 20000038844A
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South Korea
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mung bean
preparation example
bean extract
extract
acetone
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KR1019980053972A
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Korean (ko)
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KR100463081B1 (en
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김범준
김정하
조병기
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유상옥
주식회사 코리아나화장품
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/02Preparations for care of the skin for chemically bleaching or whitening the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95

Abstract

PURPOSE: Mung extract has excellent inhibiting activity of melanin formation. A whitening cosmetic base composition comprising mung bean extract as a whitening component has an excellent clinical whitening effect, and improves the stability thereof in preparation and the safety on the skin. CONSTITUTION: A mung bean extract can be prepared by the following steps of: adding water, acetone, ethylacetate or 1,3 butyleneglycol aqueous solution in the powder of mung bean; heating the solution at 50-90°C for 1-20 hrs( or 5-40°C for 1-15 days) in the extractor installed with a cooling condenser; and decompression concentration of the extract solvent in the distillatory equipment.

Description

녹두추출물을 함유하는 미백화장료 조성물Whitening cosmetic composition containing mung bean extract

본 발명은 녹두추출물을 함유하는 미백화장료 조성물에 관한 것으로, 더욱 상세하게는 녹두(Phaseolus aureus, Phaseolus radiatus, Mung bean) 추출물을 함유하여 멜라닌 생성을 억제함으로써 미백효능을 갖는 미백화장료 조성물에 관한 것이다.The present invention relates to a whitening cosmetic composition containing mung bean extract, and more particularly, to a whitening cosmetic composition having a whitening effect by inhibiting melanin production by containing mung bean (Phaseolus aureus, Phaseolus radiatus, Mung bean) extract.

피부의 흑화는 내적,외적 요인에 대한 피부세포의 반응에 의하여 발생하는 것으로, 대표적인 요인이 피부가 자외선에 노출됨으로 인한 것이다. 즉, 피부가 자외선에 노출되면 티로시나아제가 활성화되는데, 이것이 피부조직에 존재하는 아미노산의 일종인 티로신에 작용하여 도파를 생성시키고 다시 도파퀴논을 생성시키는 산화과정을 통해 피부색소세포인 멜라노사이트(Melanocyte)내의 멜라노좀(Melanosome)에서 멜라닌이라는 중합체가 합성되며, 이 멜라닌은 피부의 각질형성세포인 케라티노사이트(Keratinocyte)로 전달되어 각질화과정에 의해 피부 표면에 도달하여 자외선으로부터 피부를 보호하게 된다. 따라서, 멜라닌은 우리 인체에 없어서는 안되는 자외선 방어제이며, 또한, 단백질, 지질, 핵산 등과 같은 생체 성분을 변형시키는 다양한 라디칼을 제거하는 효과적인 프리라디칼 소거제의 역할을 감당하고 있으나, 멜라닌이 국소적으로 과도하게 합성되거나, 피부병변 및 노화에 따른 피부의 생리기능이 떨어지게 되면 멜라닌이 피부 표면에 침착되어 기미, 주근깨 및 다양한 색소침착을 유발하게 된다.Blackening of the skin is caused by the response of skin cells to internal and external factors, a typical factor being skin exposure to ultraviolet rays. In other words, when the skin is exposed to ultraviolet light, tyrosinase is activated, which acts on tyrosine, a type of amino acid present in skin tissues, which produces dopa, and then dopaquinone. In the melanosomes in the), a polymer called melanin is synthesized, and the melanin is transferred to keratinocytes, keratinocytes of the skin, and reaches the surface of the skin by keratinization to protect the skin from ultraviolet rays. Thus, melanin is an indispensable UV protective agent in our body and also serves as an effective free radical scavenger that removes various radicals that modify biological components such as proteins, lipids, nucleic acids, etc. When excessively synthesized or skin physiological function is reduced due to skin lesions and aging, melanin is deposited on the skin surface, causing melasma, freckles and various pigmentation.

이러한 피부흑화의 원인과 기작이 밝혀지면서, 피부흑화 과정에 관여하는 효소인 티로시나제(Tyrosinase)의 활성 저해효과를 갖는 물질들을 화장료에 배합하거나, 멜라닌 생성과정 중의 일부 반응을 저해함으로써 멜라닌의 생성을 감소시키는 방법이 일반적으로 사용되고 있다. 이러한 목적을 위해 사용되고 있는 대표적인 물질로는 아스코르빈산, 코직산, 히드로퀴논 등과 상백피추출물, 감초추출물 등의 식물추출물이 있다. 그러나, 코직산은 높은 티로시나아제 활성 저해작용을 갖는 반면 화장품에 배합시 변색 및 경시변화에 따른 역가 저하 등의 안정성에 문제점을 가지며 피부자극이 커 사용상의 한계를 갖고, 아스코르빈산은 티로시나제 활성 저해효과가 낮고 분자 자체의 안정성이 낮아 멜라닌 생성 억제제로 적합하지 않으며, 히드로퀴논은 피부에 대한 자극이 높아 안전성 문제로 현재 화장품 배합시에 그 사용성이 제한되고 있다. 또한, 대부분의 식물추출물들은 고농도에서만 비교적 높은 티로시나아제 활성 저해효과를 나타내며, 저농도에서는 저해 효과가 거의 나타나지 않는 등의 문제점을 갖고 있다. 특히, 기존 멜라닌 생성 억제제들의 가장 큰 문제점은 실제 사람 피부에 대한 임상적인 실험시 미백 효과가 미약하다는 점에 있다.As the causes and mechanisms of skin blackening have been revealed, it is possible to reduce the production of melanin by incorporating substances having an inhibitory effect of tyrosinase, an enzyme involved in skin blackening, into cosmetics or inhibiting some reactions during the production of melanin. The method of making is generally used. Representative materials used for this purpose include ascorbic acid, kojic acid, hydroquinone and the like, plant extracts such as lettuce extract, licorice extract. However, kojic acid has high tyrosinase activity inhibitory effect, but has a problem in stability such as lowering of titer due to discoloration and change over time when combined with cosmetics and has a limitation on use due to large skin irritation, and ascorbic acid inhibits tyrosinase activity. The low effect and low stability of the molecule itself are not suitable as inhibitors of melanin production, and hydroquinone has high irritation to the skin. In addition, most plant extracts have a relatively high inhibitory effect on tyrosinase activity only at high concentrations, and at low concentrations, there is a problem such that little inhibition effect. In particular, the biggest problem of the existing melanogenesis inhibitors is that the whitening effect is weak in clinical experiments on human skin.

이에, 본 발명자들은 기존의 멜라닌 생성 억제제들이 갖고 있는 문제점인 미약한 임상 미백효과를 극복하고, 보다 우수한 멜라닌 생성 억제 효과를 갖는 미백화장료를 제공하기 위하여 한방약 또는 민간 요법에 이용되고 있는 천연 식물을 대상으로 미백 효능이 있는 물질에 대하여 예의 연구하던 중 녹두(Phaseolus aureus, Phaseolus radiatus, Nung bean) 추출물이 우수한 멜라닌 생성 억제효과를 갖고 있음을 발견하고 본 발명을 완성하게 되었다.Accordingly, the inventors of the present invention target natural plants that are used in herbal medicine or folk remedies to overcome the weak clinical whitening effect, which is a problem of existing melanin production inhibitors, and to provide a whitening cosmetic having a superior melanin production inhibitory effect. As a result of intensive research on a substance having a whitening effect, it was found that mung bean (Phaseolus aureus, Phaseolus radiatus, Nung bean) extract has an excellent melanin production inhibitory effect and completed the present invention.

따라서, 본 발명의 목적은 녹두추출물을 함유하는 미백화장료 조성물을 제공하는 것이다.Accordingly, it is an object of the present invention to provide a whitening cosmetic composition containing mung bean extract.

상기한 목적을 달성하기 위하여, 본 발명의 미백화장료는 녹두추출물을 조성물 총 중량에 대하여 0.01∼20.0중량%의 양으로 함유함을 특징으로 한다.In order to achieve the above object, the whitening cosmetic of the present invention is characterized by containing the mung bean extract in an amount of 0.01 to 20.0% by weight based on the total weight of the composition.

상기한 본 발명의 목적, 그외의 목적, 특징 및 장점은 하기 발명의 상세한 설명으로부터 당업자에게 명백하게 드러날 것이다.The above and other objects, features and advantages of the present invention will become apparent to those skilled in the art from the following detailed description.

이하 본 발명을 보다 상세하게 설명한다.Hereinafter, the present invention will be described in more detail.

녹두(Phaseolus aureus, Phaseolus radiatus, Mung bean)는 동의보감 등의 문헌에 나타난 피부미용과 관련된 처방을 보면 녹두분말로 만들어 피부 미용효과를 얻을 수 있다고 기록되어 있으며, 최근에는 녹두를 건조 분말 형태나 물, 또는 꿀 등에 개어 피부 미용에 널리 사용되고 있는데, 특히, 녹두 단백질과 녹두 후라보노이드 성분은 세안에 효과적이며, 생리활성 성분인 비텍신과 이소비텍신은 항산화효과가 뛰어나 화장료에 함유되어 피부노화 방지 효과를 갖는다는 등의 사실이 알려져 있다. 그러나, 녹두추출물을 기미, 주근깨 및 다양한 색소침착 제거를 목적으로 미백화장료에 사용한 예는 없다.Mung bean (Phaseolus aureus, Phaseolus radiatus, Mung bean) has been documented in the literature related to skin care that can be obtained by making mung bean powder to obtain skin beauty effects. Or it is widely used in skin care by nectar, etc. In particular, mung bean protein and mung bean flavonoids are effective for face washing, and the bioactive ingredients, bitexin and isobitaxin, have excellent antioxidant effects and are contained in cosmetics to prevent skin aging. The fact is known. However, there are no examples of using mung bean extract in whitening cosmetics for the purpose of removing spots, freckles and various pigmentation.

본원 발명은 이러한 녹두추출물이 멜라닌 생성 억제 효능을 가진다는 것을 발견한 것으로, 녹두추출물은 깨끗한 물로 세척한 녹두를 건조, 거피한 후 미세하게 분말화하고, 분말화한 녹두분말에 물, 또는 아세톤, 에틸아세테이트 또는 1,3-부틸렌글리콜 수용액을 녹두분말 건조중량에 대하여 1∼15부피배의 양으로 부가한 다음, 냉각콘덴서가 부착된 추출기에서 50∼90℃ 온도로 1∼20시간동안 가열한 후, 추출용매를 냉각콘덴서가 부착된 증류장치로 감압농축하여 얻어지거나; 상기 녹두분말에 물, 또는 아세톤, 에틸아세테이트 또는 1,3-부틸렌글리콜의 수용액을 녹두분말 건조중량에 대하여 1∼15부피배의 양으로 부가한 다음, 5∼40℃ 온도로 1∼15일간 침적시켜 유효성분을 추출한 후, 추출용매를 냉각콘덴서가 부착된 증류장치로 감압농축하여 얻어진다. 이러한 녹두추출물은 강한 멜라닌 생성 억제능을 나타낼 뿐만 아니라 제품으로서의 안정성 및 피부에서의 안전성 또한 우수하다.The present invention has been found that such mung bean extract has melanin inhibitory effect, mung bean extract is finely powdered after drying and peeling the mung bean washed with clean water, water or acetone in the powdered mung bean powder, Ethyl acetate or 1,3-butylene glycol aqueous solution was added in an amount of 1 to 15 parts by volume based on the dry weight of mung bean powder, and then heated at 50 to 90 ° C. for 1 to 20 hours in an extractor equipped with a cooling capacitor. Then, the extraction solvent is obtained by concentrating under reduced pressure with a distillation apparatus equipped with a cooling capacitor; Water, or an aqueous solution of acetone, ethyl acetate or 1,3-butylene glycol is added to the mung bean powder in an amount of 1 to 15 parts by volume based on the dry weight of the mung bean powder, and then at a temperature of 5 to 40 ° C. for 1 to 15 days. After dipping and extracting the active ingredient, the extraction solvent is obtained by concentrating under reduced pressure with a distillation apparatus equipped with a cooling capacitor. These mung bean extracts not only exhibit a strong melanin inhibitory activity, but also excellent stability as a product and safety on the skin.

본 발명에서 목적하는 효과를 얻기 위하여 상기 녹두추출물은 조성물 총 중량에 대하여 0.01∼20.0중량%, 바람직하게는 0.1∼10.0중량%의 양으로 함유된다.In order to obtain the desired effect in the present invention, the mung bean extract is contained in an amount of 0.01 to 20.0% by weight, preferably 0.1 to 10.0% by weight, based on the total weight of the composition.

또한, 본 발명의 미백화장료는 그 제형에 있어서 특별히 한정되는 바가 없으며, 예를 들면, 유연화장수, 수렴화장수, 영양화장수, 아이크림, 영양크림, 맛사지크림, 클렌징크림, 클렌징폼, 클렌징워터, 파우더, 엣센스, 팩 등의 제형을 가질 수 있다.In addition, the whitening cosmetic of the present invention is not particularly limited in the formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder It may have a formulation such as, essence, pack and the like.

그리고, 각 제형의 미백화장료 조성물에 있어서, 상기한 녹두추출물 외에 다른 성분들을 기타 화장료의 제형 또는 사용목적 등에 따라 당업자가 어려움없이 적의선정하여 배합할 수 있다.In addition, in the whitening cosmetic composition of each formulation, other ingredients in addition to the above-mentioned mung bean extract can be appropriately selected and blended by those skilled in the art without difficulty according to the formulation or use purpose of other cosmetics.

이하, 녹두추출물의 제조방법을 간단히 나타낸 후, 실시예 및 비교예를 들어 본 발명의 구성 및 효과를 상세히 설명한다. 그러나, 본 발명이 이들 실시예에만 한정되는 것은 아니다.Hereinafter, after briefly showing the preparation method of the mung bean extract, the configuration and effects of the present invention will be described in detail with reference to Examples and Comparative Examples. However, the present invention is not limited only to these examples.

[제조예 1][Production Example 1]

정제수로 세척하고 건조, 거피한 녹두 1㎏을 분쇄하고, 이 분쇄물에 25% 아세톤 2ℓ를 부가한 다음 냉각콘덴서가 부착된 추출기에서 80℃ 온도에서 3시간동안 가열하여 추출하였다. 이 추출물을 300메쉬 여과포로 여과한 다음, 15℃온도에서 7일간 방치하여 저온숙성시킨 후 와트만 2번 여과지로 여과하고, 냉각콘덴서가 달린 증류장치로 60℃온도에서 감압농축하여 건조중량 53.7g의 녹두추출물을 얻었다.After washing with purified water, 1 kg of dried, peeled mung beans were ground, 2 liters of 25% acetone was added to the ground product, and the mixture was extracted by heating at 80 ° C. for 3 hours in an extractor equipped with a cooling capacitor. The extract was filtered with a 300 mesh filter cloth, left at 15 ° C. for 7 days, and then matured at low temperature. Then, the extract was filtered with Whatman No. 2 filter paper, concentrated under reduced pressure at 60 ° C. using a distillation apparatus equipped with a cooling capacitor, and dried at 53.7 g. Mung bean extract was obtained.

[제조예 2][Production Example 2]

추출용매로서 25% 아세톤 대신에 50% 아세톤을 사용하는 점만을 제외하고는 제조예 1에서와 동일한 방법으로 제조하여 건조중량 44.1g의 녹두추출물을 얻었다.Except for using 50% acetone instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 1 to obtain a dry weight of 44.1g mung bean extract.

[제조예 3][Manufacture example 3]

추출용매로서 25% 아세톤 대신에 75% 아세톤을 사용하는 점만을 제외하고는 제조예 1에서와 동일한 방법으로 제조하여 건조중량 35.2g의 녹두추출물을 얻었다.Except for the use of 75% acetone instead of 25% acetone as the extraction solvent was prepared in the same manner as in Preparation Example 1 to obtain a dry weight of 35.2g mung bean extract.

[제조예 4][Production Example 4]

추출용매로서 25% 아세톤 대신에 100% 아세톤을 사용하는 점만을 제외하고는 제조예 1에서와 동일한 방법으로 제조하여 건조중량 15.8g의 녹두추출물을 얻었다.Except for using 100% acetone instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 1 to obtain a dry weight of 15.8g mung bean extract.

[제조예 5]Production Example 5

추출용매로서 25% 아세톤 대신에 25% 에틸아세테이트를 사용하는 점만을 제외하고는 제조예 1에서와 동일한 방법으로 제조하여 건조중량 66.4g의 녹두추출물을 얻었다.Except for using 25% ethyl acetate instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 1 to obtain a dry weight of 66.4g mung bean extract.

[제조예 6][Manufacture example 6]

추출용매로서 25% 아세톤 대신에 50% 에틸아세테이트를 사용하는 점만을 제외하고는 제조예 1에서와 동일한 방법으로 제조하여 건조중량 47.8g의 녹두추출물을 얻었다.Except for using 50% ethyl acetate instead of 25% acetone as the extraction solvent was prepared in the same manner as in Preparation Example 1 to obtain a dry weight of 47.8g mung bean extract.

[제조예 7][Manufacture example 7]

추출용매로서 25% 아세톤 대신에 75% 에틸아세테이트를 사용하는 점만을 제외하고는 제조예 1에서와 동일한 방법으로 제조하여 건조중량 39.9g의 녹두추출물을 얻었다.Except for using 75% ethyl acetate instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 1 to obtain a dry weight of 39.9g mung bean extract.

[제조예 8][Manufacture example 8]

추출용매로서 25% 아세톤 대신에 100% 에틸아세테이트를 사용하는 점만을 제외하고는 제조예 1에서와 동일한 방법으로 제조하여 건조중량 31.3g의 녹두추출물을 얻었다.Except for using 100% ethyl acetate instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 1 to obtain a dry weight of 31.3g mung bean extract.

[제조예 9][Manufacture example 9]

추출용매로서 25% 아세톤 대신에 25% 1,3-부틸렌글리콜을 사용하는 점만을 제외하고는 제조예 1에서와 동일한 방법으로 제조하여 건조중량 95.8g의 녹두추출물을 얻었다.Except for using 25% 1,3-butylene glycol instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 1 to obtain a dry weight of 95.8g mung bean extract.

[제조예 10][Production Example 10]

추출용매로서 25% 아세톤 대신에 50% 1,3-부틸렌글리콜을 사용하는 점만을 제외하고는 제조예 1에서와 동일한 방법으로 제조하여 건조중량 76.3g의 녹두추출물을 얻었다.Except for using 50% 1,3-butylene glycol instead of 25% acetone as the extraction solvent was prepared in the same manner as in Preparation Example 1 to obtain a dry weight of 76.3g of mung bean extract.

[제조예 11][Production Example 11]

추출용매로서 25% 아세톤 대신에 75% 1,3-부틸렌글리콜을 사용하는 점만을 제외하고는 제조예 1에서와 동일한 방법으로 제조하여 건조중량 59.4g의 녹두추출물을 얻었다.Except for using 75% 1,3-butylene glycol instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 1 to obtain a dry weight of 59.4g of mung bean extract.

[제조예 12][Manufacture example 12]

추출용매로서 25% 아세톤 대신에 100% 1,3-부틸렌글리콜을 사용하는 점만을 제외하고는 제조예 1에서와 동일한 방법으로 제조하여 건조중량 23.0g의 녹두추출물을 얻었다.Except for using 100% 1,3-butylene glycol instead of 25% acetone as the extraction solvent was prepared in the same manner as in Preparation Example 1 to obtain a dry weight 23.0g mung bean extract.

[제조예 13][Production Example 13]

정제수로 세척하고 건조, 거피한 녹두 1㎏을 분쇄하고, 이 분쇄물에 25% 아세톤 5ℓ를 부가한 다음, 30℃온도에서 3일간 침적시켜 추출하였다. 이 추출물을 300메쉬 여과포로 여과한 다음, 15℃온도에서 7일간 방치하여 저온숙성시킨 후 와트만 2번 여과지로 여과하고, 냉각콘덴서가 달린 증류장치로 60℃온도에서 감압농축하여 건조중량 45.4g의 녹두추출물을 얻었다.After washing with purified water, 1 kg of dried, peeled mung beans were ground, 5 L of 25% acetone was added to the ground product, and the mixture was immersed at 30 ° C. for 3 days and extracted. The extract was filtered with a 300 mesh filter cloth, left at 15 ° C. for 7 days, and then matured at low temperature. Then, the extract was filtered using Whatman No. 2 filter paper, and concentrated under reduced pressure at 60 ° C. using a distillation apparatus equipped with a cooling capacitor to dry weight 45.4 g. Mung bean extract was obtained.

[제조예 14]Production Example 14

추출용매로서 25% 아세톤 대신에 50% 아세톤을 사용하는 점만을 제외하고는 제조예 13에서와 동일 방법으로 제조하여 건조중량 34.4g의 녹두추출물을 얻었다.Except for using 50% acetone instead of 25% acetone as the extraction solvent was prepared in the same manner as in Preparation Example 13 to obtain a dry weight of 34.4g mung bean extract.

[제조예 15][Production Example 15]

추출용매로서 25% 아세톤 대신에 75% 아세톤을 사용하는 점만을 제외하고는 제조예 13에서와 동일 방법으로 제조하여 건조중량 33.1g의 녹두추출물을 얻었다.Except for using 75% acetone instead of 25% acetone as the extraction solvent was prepared in the same manner as in Preparation Example 13 to obtain a dry weight 33.1g mung bean extract.

[제조예 16][Production Example 16]

추출용매로서 25% 아세톤 대신에 100% 아세톤을 사용하는 점만을 제외하고는 제조예 13에서와 동일 방법으로 제조하여 건조중량 18.9g의 녹두추출물을 얻었다.Except for using 100% acetone instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 13 to obtain a dry weight of 18.9g mung bean extract.

[제조예 17][Production Example 17]

추출용매로서 25% 아세톤 대신에 25% 에틸아세테이트를 사용하는 점만을 제외하고는 제조예 13에서와 동일한 방법으로 제조하여 건조중량 58.8g의 녹두추출물을 얻었다.Except for using 25% ethyl acetate instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 13 to obtain a dry weight of 58.8g mung bean extract.

[제조예 18][Production Example 18]

추출용매로서 25% 아세톤 대신에 50% 에틸아세테이트를 사용하는 점만을 제외하고는 제조예 13에서와 동일한 방법으로 제조하여 건조중량 48.1g의 녹두추출물을 얻었다.Except for using 50% ethyl acetate instead of 25% acetone as the extraction solvent was prepared in the same manner as in Preparation Example 13 to obtain a dry weight of 48.1g mung bean extract.

[제조예 19][Production Example 19]

추출용매로서 25% 아세톤 대신에 75% 에틸아세테이트를 사용하는 점만을 제외하고는 제조예 13에서와 동일한 방법으로 제조하여 건조중량 23.9g의 녹두추출물을 얻었다.Except for using 75% ethyl acetate instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 13 to obtain a dry bean extract of 23.9g dry weight.

[제조예 20][Production Example 20]

추출용매로서 25% 아세톤 대신에 100% 에틸아세테이트를 사용하는 점만을 제외하고는 제조예 13에서와 동일한 방법으로 제조하여 건조중량 15.2g의 녹두추출물을 얻었다.Except for using 100% ethyl acetate instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 13 to obtain a dry weight of 15.2g mung bean extract.

[제조예 21][Production Example 21]

추출용매로서 25% 아세톤 대신에 25% 1,3-부틸렌글리콜을 사용하는 점만을 제외하고는 제조예 13에서와 동일한 방법으로 제조하여 건조중량 91.2g의 녹두추출물을 얻었다.A green bean extract having a dry weight of 91.2 g was obtained in the same manner as in Preparation Example 13, except that 25% 1,3-butylene glycol was used instead of 25% acetone as the extraction solvent.

[제조예 22][Production Example 22]

추출용매로서 25% 아세톤 대신에 50% 1,3-부틸렌글리콜을 사용하는 점만을 제외하고는 제조예 13에서와 동일한 방법으로 제조하여 건조중량 73.5g의 녹두추출물을 얻었다.Except for using 50% 1,3-butylene glycol instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 13 to obtain a dry weight of 73.5g of mung bean extract.

[제조예 23][Manufacture example 23]

추출용매로서 25% 아세톤 대신에 75% 1,3-부틸렌글리콜을 사용하는 점만을 제외하고는 제조예 13에서와 동일한 방법으로 제조하여 건조중량 53.8g의 녹두추출물을 얻었다.Except for using 75% 1,3-butylene glycol instead of 25% acetone as the extraction solvent was prepared in the same manner as in Preparation Example 13 to obtain a dry weight of 53.8g mung bean extract.

[제조예 24][Manufacture example 24]

추출용매로서 25% 아세톤 대신에 100% 1,3-부틸렌글리콜을 사용하는 점만을 제외하고는 제조예 13에서와 동일한 방법으로 제조하여 건조중량 30.9g의 녹두추출물을 얻었다.Except for using 100% 1,3-butylene glycol instead of 25% acetone as an extraction solvent was prepared in the same manner as in Preparation Example 13 to obtain a dry weight of 30.9g mung bean extract.

[시험예 1] : 티로시나제 활성 억제능 시험Test Example 1 Tyrosinase Activity Inhibitory Activity Test

먼저 기질인 티로신을 0.05M 인산나트륨 완충용액(pH 6.8)에 녹여 0.1㎎/㎖의 용액(기질액)을 준비하고, 녹두추출물 100㎎을 50% 1,3-부틸렌글리콜 10㎖에 용해시키고 다시 완충용액에 희석하여 200㎍/㎖로 조절하여 추출물 시료액을 준비하였다. 기질액 0.5㎖를 시험관에 넣은 다음, 추출물 시료액 0.5㎖를 가하고 37℃ 항온조에서 10분간 반응시킨 후, 버섯류(Mushroom)로부터 추출한 200U/㎖ 티로시나아제 용액(SIGMA사 제품) 0.5㎖씩을 가하여 교반한 다음, 다시 37℃ 항온조에서 10분간 반응시켰다. 이 반응액이 든 시험관을 얼음 위에 놓아 급냉시켜 반응을 중지시키고 분광광도계(spectrophotometer)로 475nm에서 흡광도를 측정하여 하기 수학식 1에 따라 티로시나제 활성 저해율을 계산한 후, 그 결과를 표 1에 나타내었다. 이때 녹두추출물 대신에 완충용액 0.5㎖를 사용한 것을 대조군으로 하여 동일한 방법에 의해 흡광도를 측정하여 이를 이용하여 티로시나제 활성 억제능을 계산하였다.First, tyrosine, a substrate, was dissolved in 0.05 M sodium phosphate buffer (pH 6.8) to prepare a 0.1 mg / ml solution (substrate), and 100 mg of mung bean extract was dissolved in 10 ml of 50% 1,3-butylene glycol. Dilution in the buffer solution was adjusted to 200 ㎍ / ㎖ to prepare an extract sample solution. 0.5 ml of substrate solution was added to a test tube, 0.5 ml of extract sample solution was added thereto, and reacted for 10 minutes in a 37 ° C thermostat. Then, 0.5 ml of 200 U / ml tyrosinase solution (manufactured by SIGMA) extracted from mushrooms was added and stirred. Then, it was reacted for 10 minutes in a 37 ℃ thermostat. After the test tube containing the reaction solution was placed on ice to quench the reaction, the absorbance was measured at 475 nm with a spectrophotometer, and the tyrosinase activity inhibition rate was calculated according to Equation 1 below, and the results are shown in Table 1. . In this case, the absorbance was measured by the same method using 0.5 ml of buffer instead of mung bean extract as a control, and the tyrosinase activity inhibitory activity was calculated using the same.

(실험농도: 200㎍/㎖)(Experimental concentration: 200 µg / ml) 실험물질Experimental substance 티로시나제 활성 저해율(%)Tyrosinase activity inhibition rate (%) 실험물질Experimental substance 티로시나제 활성 저해율(%)Tyrosinase activity inhibition rate (%) 제조예 1Preparation Example 1 13.513.5 제조예 13Preparation Example 13 15.315.3 제조예 2Preparation Example 2 17.317.3 제조예 14Preparation Example 14 19.219.2 제조예 3Preparation Example 3 20.220.2 제조예 15Preparation Example 15 24.524.5 제조예 4Preparation Example 4 24.124.1 제조예 16Preparation Example 16 30.730.7 제조예 5Preparation Example 5 11.411.4 제조예 17Preparation Example 17 20.820.8 제조예 6Preparation Example 6 28.728.7 제조예 18Preparation Example 18 37.937.9 제조예 7Preparation Example 7 33.933.9 제조예 19Preparation Example 19 43.543.5 제조예 8Preparation Example 8 30.430.4 제조예 20Preparation Example 20 45.345.3 제조예 9Preparation Example 9 34.534.5 제조예 21Preparation Example 21 38.438.4 제조예 10Preparation Example 10 42.342.3 제조예 22Preparation Example 22 53.453.4 제조예 11Preparation Example 11 48.648.6 제조예 23Preparation Example 23 57.057.0 제조예 12Preparation Example 12 46.646.6 제조예 24Preparation Example 24 57.457.4

이하, 상기 제조예 22에서 얻은 녹두추출물의 농도를 5, 25, 50, 100, 200 및 500㎍/㎖로 조정하여, 농도별 티로시나제 활성 저해율을 구하고, 그 결과를 하기 표 2에 나타내었다.Hereinafter, the concentrations of mung bean extracts obtained in Preparation Example 22 were adjusted to 5, 25, 50, 100, 200, and 500 µg / ml to determine the inhibition rate of tyrosinase activity for each concentration, and the results are shown in Table 2 below.

실험농도(㎍/㎖)Experimental concentration (㎍ / ㎖) 티로시나제 활성 저해율(%)Tyrosinase activity inhibition rate (%) 55 7.57.5 2525 13.813.8 5050 24.224.2 100100 33.833.8 200200 53.453.4 500500 68.768.7

상기 표 2에서 알 수 있는 바와 같이, 티로시나제 활성을 50% 저해하는 녹두추출물의 농도(IC50)는 약 182.7㎍/㎖로, 코직산이 약 5.8㎍/㎖, 알부틴이 약 45.3㎍/㎖, 상지추출물이 약 0.5㎍/㎖인 것을 고려해 볼 때 녹두추출물의 미백효과는 좋지 않다.As can be seen in Table 2, the concentration (IC 50 ) of mung bean extract that inhibits tyrosinase activity by 50% is about 182.7 µg / ml, about 5.8 µg / ml of kojic acid, about 45.3 µg / ml of arbutin, and upper limbs. Considering that the extract is about 0.5µg / ml, the whitening effect of mung bean extract is not good.

[시험예 2] : 멜라닌 생성 억제능 시험Test Example 2 melanin production inhibitory test

25㎠ T-플라스크에 DMEM 배지(포도당 4.5g/ℓ 89%, 혈청 10%, 항생제 1%) 5∼6㎖를 넣은 후, 동결보관중인 마우스 유래 B-16 멜라노마(ATCC CRL 6323) 세포주를 접종하여 37℃ 온도, 5% CO2조건하에서 24시간동안 배양한 다음, 0.02% EDTA가 함유된 0.05% 트립신을 처리하여 세포를 분리한 후, 25㎠ T-플라스크에 접종하여 48시간동안 배양하였다. 그 결과, 얻어진 세포수는 플라스크 당 5.76×106cells이었다. 여기에 100μg/㎖의 녹두추출물을 DMEM 배지에 희석시켜 배양된 멜라노마 세포에 처리하여 37℃에서 5일간 배양하였다. 배양 후 배지를 모두 제거하고, 0.02% EDTA와 0.05% 트립신을 함유한 살린-포스페이트 완충용액(PBS) 1㎖를 처리하여 세포를 분리시킨 후 5분간 원심분리하여 세포만을 수집하였다. 수집된 세포에 5% 트리클로로아세테이트(TCA)를 처리하여 교반하고, 원심분리하여 침전된 멜라닌을 살린-포스페이트 완충용액으로 세척하였다. 이어서, 침전된 멜라닌에 1N NaOH를 처리하여 용해시킨 후 475nm에서 흡광도를 측정하고, 합성 멜라닌(시그마사)의 표준농도 곡선으로부터 멜라닌의 농도를 결정한 후, 멜라닌 생성 억제율을 하기 표 3에 나타내었다.After 5-6 ml of DMEM medium (4.5 g of glucose, 10% of serum, 1% of antibiotics) was placed in a 25 cm2 T-flask, the mouse-derived B-16 melanoma (ATCC CRL 6323) cell line was frozen. Incubated for 24 hours at 37 ℃ temperature, 5% CO 2 conditions, and then treated with 0.05% trypsin containing 0.02% EDTA to isolate the cells, incubated in 25 cm 2 T-flask and incubated for 48 hours. . As a result, the cell number obtained was 5.76 × 10 6 cells per flask. Here, 100 μg / mL mung bean extract was diluted in DMEM medium, treated with cultured melanoma cells, and cultured at 37 ° C. for 5 days. After incubation, all of the medium was removed, and the cells were separated by treatment with 1 ml of saline-phosphate buffer solution (PBS) containing 0.02% EDTA and 0.05% trypsin, followed by centrifugation for 5 minutes to collect only cells. The collected cells were treated with 5% trichloroacetate (TCA), stirred, and centrifuged to wash the precipitated melanin with saline-phosphate buffer. Subsequently, 1N NaOH was dissolved and dissolved in the precipitated melanin, the absorbance was measured at 475 nm, and the melanin concentration was determined from the standard concentration curve of the synthetic melanin (Sigma).

(실험농도: 100㎍/㎖)(Experimental concentration: 100 µg / ml) 실험물질Experimental substance 멜라닌 생성 억제율(%)Melanin production inhibition rate (%) 실험물질Experimental substance 멜라닌 생성 억제율(%)Melanin production inhibition rate (%) 제조예 1Preparation Example 1 38.038.0 제조예 13Preparation Example 13 45.145.1 제조예 2Preparation Example 2 45.745.7 제조예 14Preparation Example 14 49.849.8 제조예 3Preparation Example 3 59.559.5 제조예 15Preparation Example 15 55.355.3 제조예 4Preparation Example 4 54.254.2 제조예 16Preparation Example 16 51.751.7 제조예 5Preparation Example 5 21.621.6 제조예 17Preparation Example 17 34.034.0 제조예 6Preparation Example 6 36.736.7 제조예 18Preparation Example 18 38.938.9 제조예 7Preparation Example 7 44.844.8 제조예 19Preparation Example 19 48.748.7 제조예 8Preparation Example 8 45.045.0 제조예 20Preparation Example 20 49.349.3 제조예 9Preparation Example 9 33.633.6 제조예 21Preparation Example 21 49.249.2 제조예 10Preparation Example 10 62.562.5 제조예 22Preparation Example 22 72.772.7 제조예 11Preparation Example 11 69.269.2 제조예 23Preparation Example 23 76.076.0 제조예 12Preparation Example 12 66.566.5 제조예 24Preparation Example 24 79.879.8 비교제조예 1Comparative Production Example 1 50.950.9

이하, 녹두추출물 무처리군 및 상기 제조예 22에서 얻은 녹두추출물의 농도를 10, 50, 100 및 200㎍/㎖로 조절하여, 농도별 멜라닌 함량 및 멜라닌 생성 억제율을 구하고, 그 결과를 하기 표 4 및 표 5에 나타내었다. 한편, 녹두 추출물 대신에 코직산을 실험물질로 하여 동일 실험을 실시하고, 그 결과를 하기 표 4와 5에 비교제조예로서 기재하였다.Hereinafter, by adjusting the concentration of mung bean extract untreated group and the mung bean extract obtained in Preparation Example 22 to 10, 50, 100 and 200 ㎍ / ㎖, to determine the melanin content and the inhibition rate of melanin production by concentration, the results are shown in Table 4 And in Table 5. Meanwhile, the same experiment was carried out using kojic acid as a test substance instead of mung bean extract, and the results are described as Comparative Preparation Examples in Tables 4 and 5 below.

실험농도(㎍/㎖)Experimental concentration (㎍ / ㎖) 멜라닌 함량(ρg/cell)Melanin content (ρg / cell) 제조예 22Preparation Example 22 비교제조예 1Comparative Production Example 1 무처리군No treatment group 4.284.28 4.284.28 1010 2.572.57 2.322.32 5050 2.092.09 2.252.25 100100 1.171.17 2.102.10 200200 0.760.76 2.012.01

실험농도(㎍/㎖)Experimental concentration (㎍ / ㎖) 멜라닌 생성 억제율(%)Melanin production inhibition rate (%) 제조예 22Preparation Example 22 비교제조예 1Comparative Production Example 1 무처리군No treatment group -- -- 1010 40.040.0 45.845.8 5050 51.251.2 47.447.4 100100 72.772.7 50.950.9 200200 82.282.2 53.053.0

상기 표 4 및 표 5에서 알 수 있는 바와 같이, 멜라닌을 생성하는 세포인 멜라노사이트의 세포배양에서는 녹두추출물의 멜라닌 생성 억제능이 우수함을 알 수 있다.As can be seen in Table 4 and Table 5, it can be seen that the cell culture of melanocytes that produce melanin is excellent in melanin production inhibitory ability of mung bean extract.

이상의 시험예 1 및 2를 종합하여 볼 때, 녹두추출물은 피부 흑화의 반응 기작 중 티로시나제의 활성을 저해하는데 관여하는 것이 아니라, 멜라닌 생성과정 중의 일부 반응을 저해함으로써 멜라닌 생성을 감소시키는 역할을 함을 알 수 있다.Taken together, Test Example 1 and 2 above, mung bean extract is not involved in inhibiting the activity of tyrosinase in the reaction mechanism of skin blackening, but rather plays a role in reducing melanin production by inhibiting some reactions in melanogenesis. Able to know.

이하, 상기 제조예 1 내지 24에서 얻은 녹두추출물을 3.0중량%의 양으로 함유하는 미백화장료(실시예 1) 및 코직산을 0.8중량%의 양으로 함유하는 미백화장료(비교실시예 1)를 제조한 후, 미백효능 시험을 실시하였다.Hereinafter, a whitening cosmetic material (Example 1) containing the mung bean extract obtained in Preparation Examples 1 to 24 in an amount of 3.0% by weight and a whitening cosmetic material (comparative example 1) containing kojic acid in an amount of 0.8% by weight were prepared. Then, a whitening efficacy test was performed.

[실시예 1 및 비교실시예 1] : 에센스[Example 1 and Comparative Example 1]: Essence

(단위: 중량부)(Unit: parts by weight) 성분ingredient 실시예 1Example 1 비교실시예 1Comparative Example 1 녹두추출물Mung Bean Extract 3.03.0 -- 코직산Kojic acid -- 0.80.8 글리세린glycerin 10.010.0 10.010.0 베타인Betaine 5.05.0 5.05.0 PEG 1500PEG 1500 2.02.0 2.02.0 알란토인Allantoin 0.10.1 0.10.1 DL-판테놀DL-panthenol 0.30.3 0.30.3 EDTA-2NaEDTA-2Na 0.020.02 0.020.02 벤조페논-9Benzophenone-9 0.040.04 0.040.04 히드록시에틸 셀룰로우스Hydroxyethyl cellulose 0.10.1 0.10.1 소듐히아루로네이트Sodium hyaluronate 8.08.0 8.08.0 카르복시비닐폴리머Carboxy Vinyl Polymer 0.20.2 0.20.2 트리에탄올아민Triethanolamine 0.180.18 0.180.18 옥틸도데카논Octyldodecanone 0.30.3 0.30.3 옥틸도데세스-16Octyldodeceth-16 0.40.4 0.40.4 에탄올ethanol 6.06.0 6.06.0 향, 방부제, 색소Incense, preservative, coloring 미량a very small amount 미량a very small amount 정제수Purified water to 100to 100 to 100to 100

[시험예 3] : 임상실험에 의한 미백효능 시험[Test Example 3]: whitening efficacy test by clinical experiment

본 발명의 화장료의 임상 미백효능을 시험하기 위하여, 기미, 주근깨 및 색소침착증을 갖고 있는 건강한 성인 남녀 40명을 무작위로 20명씩 2개 그룹(A 및 B)으로 나눈 후, A그룹을 대상으로는 실시예 1을, B그룹을 대상으로는 비교실시예 1을 각각 1일 2회씩 12주간 도포한 후 피부색의 변화를 크로마메타(Minolta CR300)를 이용하여 색의 밝기변화(ΔL)를 측정하고, 복수의 숙련자에 의한 객관적 육안 관찰과 피검자에 의한 주관적 육안관찰을 실시하여 미백효과를 측정하였다. 그 결과를 하기 표 6에 나타내었다. 이때 미백효능 정도를 다음의 7등급으로 분류하여 평가하였다.In order to test the clinical whitening efficacy of the cosmetics of the present invention, 40 healthy adult men and women with blemishes, freckles, and pigmentation were randomly divided into two groups (A and B) of 20 people, Example 1 was applied to Group B, and Comparative Example 1 was applied twice a day for 12 weeks, respectively, and the change in skin color was measured using chromata (Minolta CR300) to measure the change in color brightness (ΔL), The whitening effect was measured by objective visual observation by a plurality of skilled workers and subjective visual observation by a subject. The results are shown in Table 6 below. At this time, the degree of whitening efficacy was classified into the following seven ratings.

·미백효능 평가 기준 :Evaluation criteria for whitening efficacy:

-3: 매우 악화 -2: 악화 -1: 약간 악화 0: 변화 없음-3: very worse -2: worse -1: slightly worse 0: no change

1: 약간 개선 2: 개선 3: 매우 개선1: slightly improved 2: improved 3: highly improved

피검자Subject 피부색의 밝기 변화(ΔL)Change in brightness of skin color (ΔL) 숙련자의 객관적 평가Objective evaluation of the skilled person 피검자의 주관적 평가Subjective Evaluation of the Subject A그룹A group B그룹B group A그룹A group B그룹B group A그룹A group B그룹B group 1One 5.35.3 2.32.3 1One 00 1One 1One 22 4.94.9 2.92.9 33 1One 22 1One 33 4.54.5 3.43.4 1One 1One 1One 1One 44 4.74.7 1.51.5 00 00 1One 1One 55 4.14.1 2.62.6 22 00 1One 00 66 4.54.5 3.83.8 22 1One 22 22 77 4.84.8 0.80.8 1One 1One 22 1One 88 3.73.7 0.30.3 33 1One 1One 22 99 5.75.7 2.72.7 00 1One 00 00 1010 3.93.9 2.22.2 1One 00 00 00 1111 4.04.0 3.63.6 1One 00 00 -1-One 1212 3.33.3 3.53.5 22 00 1One 00 1313 4.64.6 2.52.5 22 1One 33 22 1414 4.44.4 1.91.9 1One 00 1One 1One 1515 4.34.3 2.02.0 33 00 33 1One 1616 5.65.6 3.13.1 22 00 1One 00 1717 5.25.2 2.82.8 1One 00 1One 00 1818 5.05.0 2.12.1 00 00 1One 00 1919 5.35.3 3.73.7 00 00 00 00 2020 5.25.2 2.52.5 33 22 1One 00 평균값medium 4.74.7 2.52.5 1.451.45 0.450.45 1.151.15 0.60.6

상기 표 6에서 알 수 있는 바와 같이, 본 발명의 녹두추출물을 함유한 화장료 조성물을 도포한 A그룹의 평균 ΔL값은 4.7(P<0.01)이며, 코직산을 함유한 화장료 조성물을 도포한 B그룹의 평균 ΔL값은 2.5(P>0.05)이었고, 숙련자에 의한 미백평가에서는 A그룹은 1.45(P<0.01), B그룹은 0.45(P>0.05)이었으며, 피검자에 의한 미백 평가에서는 A그룹은 1.15(P<0.05), B그룹은 0.6(P>0.05)로 나타나, 이상의 결과를 종합하여 볼 때 A그룹의 임상 미백효능이 더욱 우수함을 알 수 있다.As can be seen in Table 6, the average ΔL value of the A group to which the cosmetic composition containing the mung bean extract of the present invention is applied is 4.7 (P <0.01), of the B group to which the cosmetic composition containing kojic acid is applied. The mean ΔL value was 2.5 (P> 0.05), and in the whitening evaluation by the expert, the A group was 1.45 (P <0.01), and the B group was 0.45 (P> 0.05). P <0.05) and B group showed 0.6 (P> 0.05), and it can be seen that the clinical whitening efficacy of group A is more excellent when the above results are combined.

[시험예 4] : 제형의 안정성 시험Test Example 4 Test of Stability of Formulation

본 발명의 화장료 제형의 안정성을 시험하기 위하여, 실시예 1 및 비교실시예 1을 45℃로 일정하게 유지되는 항온조에서 불투명 초자 용기에 담아 2주동안 보관하고, 또한 4℃로 일정하게 유지되는 완전히 차광된 냉장고내에서 불투명 초자 용기에 담아 2주 동안 보관한 후 변색 정도를 비교측정하였다. 그 결과를 하기 표 7에 나타내었다. 이때 제품 변색 정도는 다음의 6등급으로 분류하여 평가하였다.In order to test the stability of the cosmetic formulation of the present invention, Example 1 and Comparative Example 1 were stored in an opaque glass container for 2 weeks in a constant temperature bath at a constant temperature of 45 ℃ and kept completely at 4 ℃ After storing for 2 weeks in an opaque glass jar in a shaded refrigerator, the degree of discoloration was measured. The results are shown in Table 7 below. At this time, the degree of product discoloration was evaluated by classifying into the following six grades.

·제품 변색 평가 기준 :Product discoloration evaluation criteria:

0: 변화 없음 1: 극히 조금 변함 2: 조금 변함0: no change 1: very little change 2: little change

3: 조금 심하게 변함 4: 심하게 변함 5: 극히 심하게 변함3: slightly worse 4: very badly 5: very severely

온도Temperature 실험물질 변색 정도Discoloration degree of test substance 실시예 1Example 1 비교실시예 1Comparative Example 1 45℃45 ℃ 0.50.5 5.05.0 4℃4 ℃ 00 2.02.0

상기 표 7에서 알 수 있는 바와 같이, 본 발명의 녹두추출물을 함유한 화장료 조성물은 변색이 거의 되지 않으므로 제형으로서의 안정성이 큼을 알 수 있다.As can be seen in Table 7, the cosmetic composition containing the mung bean extract of the present invention is hardly discolored, it can be seen that the stability as a formulation is large.

[시험예 5] : 피부 자극시험[Test Example 5]: Skin irritation test

본 발명의 화장료의 피부자극 정도를 시험하기 위하여, 실시예 1 및 비교실시예 1을 패쇄첩포시험(Patch Test) 방법으로 24시간동안 적용한 후 제거하고, 이때 나타나는 피부자극 정도를 비교측정하였다. 그 결과를 하기 표 8에 나타내었다. 이때 피부자극 정도는 다음의 6등급으로 분류하여 평가하였다.In order to test the skin irritation degree of the cosmetic of the present invention, Example 1 and Comparative Example 1 were removed after applying for 24 hours by the Patch Test method, and the skin irritation degree appeared at this time was measured and compared. The results are shown in Table 8 below. At this time, skin irritation degree was evaluated by classifying into the following six grades.

·피부자극 평가 기준 :Skin irritation evaluation criteria:

0: 자극 없음 1: 자극 거의 없음 2: 자극 조금 있음0: no stimulation 1: almost no stimulation 2: little stimulation

3: 자극 조금 심함 4 : 자극 심함 5: 자극 극히 심함3: Slightly Severe Stimulus 4: Severe Stimulation 5: Severe Stimulation

경과시간Elapsed time 피부자극 정도Skin irritation degree 실시예 1Example 1 비교실시예 1Comparative Example 1 24시간24 hours 0.50.5 5.05.0 48시간48 hours 00 1.51.5

이하, 상기한 시험예들의 결과를 근거로 하여, 녹두추출물을 함유함으로써 피부미백 효과를 제공할 수 있는 여러 제형의 화장료를 조성하여 제시한다. 그러나, 본 발명의 조성물이 하기의 제형예들로 한정되는 것은 아니다.Hereinafter, based on the results of the above test examples, by presenting a cosmetic composition of various formulations that can provide a skin lightening effect by containing mung bean extract. However, the composition of the present invention is not limited to the following formulation examples.

(제형예 1) 유연화장수(스킨로션)Formulation Example 1 Softener (Skin Lotion)

배합성분Ingredient 중량부Parts by weight 녹두 추출물Mung Bean Extract 0.10.1 1,3-부틸렌글리콜1,3-butylene glycol 6.06.0 글리세린glycerin 4.04.0 올레일알코올Oleyl alcohol 0.10.1 폴리솔베이트 20Polysorbate 20 0.50.5 에탄올ethanol 15.015.0 벤조페논-9Benzophenone-9 0.050.05 향, 방부제Incense, preservative 미량a very small amount 정제수Purified water to 100to 100

(제형예 2) 영양화장수(밀크로션)Formulation Example 2 Nutrients (Milk Lotion)

배합성분Ingredient 중량부Parts by weight 녹두 추출물Mung Bean Extract 1.01.0 프로필렌글리콜Propylene glycol 6.06.0 글리세린glycerin 4.04.0 트리에탄올아민Triethanolamine 1.21.2 토코페릴아세테이트Tocopheryl Acetate 3.03.0 유동 파라핀Floating paraffin 5.05.0 스쿠알란Squalane 3.03.0 마카다미아너트오일Macadamia Nut Oil 2.02.0 폴리솔베이트 60Polysorbate 60 1.51.5 솔비탄세스퀴올레이트Sorbitan sesquioleate 1.01.0 카르복시비닐 폴리머Carboxyvinyl Polymer 1.01.0 향, 방부제Incense, preservative 미량a very small amount 정제수Purified water to 100to 100

(제형예 3) 영양크림Formulation Example 3 Nutrition Cream

배합성분Ingredient 중량부Parts by weight 녹두 추출물Mung Bean Extract 3.03.0 바셀린vaseline 7.07.0 유동파라핀Liquid paraffin 10.010.0 밀납Beeswax 2.02.0 폴리솔베이트 60Polysorbate 60 2.02.0 솔비탄세스퀴올레이트Sorbitan sesquioleate 2.52.5 스쿠알란Squalane 3.03.0 프로필렌글리콜Propylene glycol 6.06.0 글리세린glycerin 4.04.0 트리에탄올아민Triethanolamine 0.50.5 카르복시비닐폴리머Carboxy Vinyl Polymer 0.50.5 토코페릴아세테이트Tocopheryl Acetate 0.10.1 향, 방부제Incense, preservative 미량a very small amount 정제수Purified water to 100to 100

(제형예 4) 마사지크림Formulation Example 4 Massage Cream

배합성분Ingredient 중량부Parts by weight 녹두 추출물Mung Bean Extract 0.10.1 프로필렌글리콜Propylene glycol 6.06.0 글리세린glycerin 4.04.0 트리에탄올아민Triethanolamine 0.50.5 밀납Beeswax 2.02.0 토코페릴아세테이트Tocopheryl Acetate 0.10.1 폴리솔베이트 60Polysorbate 60 3.03.0 솔비탄세스퀴올레이트Sorbitan sesquioleate 2.52.5 세테아릴알코올Cetearyl Alcohol 2.02.0 유동파라핀Liquid paraffin 30.030.0 카르복시비닐폴리머Carboxy Vinyl Polymer 0.50.5 향, 방부제Incense, preservative 미량a very small amount 정제수Purified water to 100to 100

(제형예 5) 팩Formulation Example 5 Pack

배합성분Ingredient 중량부Parts by weight 녹두 추출물Mung Bean Extract 3.03.0 프로필렌글리콜Propylene glycol 2.02.0 글리세린glycerin 4.04.0 카르복시비닐 폴리머Carboxyvinyl Polymer 0.30.3 에탄올ethanol 7.07.0 피이지-40 히드로게비이티드 캐스터 오일Fiji-40 Hydrogenated Castor Oil 0.80.8 트리에탄올아민Triethanolamine 0.30.3 향, 방부제Incense, preservative 미량a very small amount 정제수Purified water to 100to 100

이상에서 설명한 바와 같이, 미백화장료의 미백성분으로서 녹두추출물을 사용함으로써, 녹두추출물의 멜라닌 생성 억제능에 의해 우수한 임상 미백효능을 나타내며 제형내에서의 불안정성 및 피부안전성을 개선시킨 미백화장료 조성물을 제공할 수 있다.As described above, by using mung bean extract as a whitening ingredient of the whitening cosmetics, it is possible to provide a whitening cosmetic composition exhibiting excellent clinical whitening effect by the melanin production inhibitory effect of mung bean extract and improving instability and skin safety in the formulation. have.

Claims (3)

조성물 총 중량에 대하여 0.01∼20.0중량%의 양으로 녹두 추출물을 함유함을 특징으로 하는 미백화장료 조성물.A whitening cosmetic composition comprising mung bean extract in an amount of 0.01 to 20.0% by weight based on the total weight of the composition. 제 1항에 있어서, 상기 녹두추출물은 녹두분말에 물, 또는 아세톤, 에틸아세테이트 또는 1,3-부틸렌글리콜 수용액을 부가한 다음, 냉각콘덴서가 부착된 추출기에서 50∼90℃ 온도로 1∼20시간동안 가열한 후, 추출용매를 냉각콘덴서가 부착된 증류장치로 감압농축하여 얻어진 것임을 특징으로 하는 미백화장료 조성물.The method of claim 1, wherein the mung bean extract is added water or acetone, ethyl acetate or 1,3-butylene glycol aqueous solution to the mung bean powder, and then 1 to 20 at a temperature of 50 to 90 ℃ in an extractor with a cooling capacitor After heating for a time, the whitening cosmetic composition, characterized in that the extraction solvent was obtained by concentrating under reduced pressure in a distillation apparatus with a cooling capacitor. 제 1항에 있어서, 상기 녹두추출물은 녹두분말에 물, 또는 아세톤, 에틸아세테이트 또는 1,3-부틸렌글리콜 수용액을 부가한 다음, 5∼40℃ 온도로 1∼15일간 침적시켜 추출한 후, 추출용매를 냉각콘덴서가 부착된 증류장치로 감압농축하여 얻어진 것임을 특징으로 하는 미백화장료 조성물.The extract of claim 1, wherein the mung bean extract is extracted by adding water, or acetone, ethyl acetate or 1,3-butylene glycol aqueous solution to the mung bean powder, and then immersing it at a temperature of 5 to 40 ° C. for 1 to 15 days. A whitening cosmetic composition, characterized in that the solvent is obtained by concentrating under reduced pressure with a distillation apparatus equipped with a cooling capacitor.
KR10-1998-0053972A 1998-12-09 1998-12-09 Cosmetic compositions containing Phaseolus aureus extracts for skin whitening KR100463081B1 (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
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KR20030000715A (en) * 2001-06-26 2003-01-06 장성환 Method wich mke powder for a beauty pack
KR20030042527A (en) * 2001-11-23 2003-06-02 주식회사 코리아나화장품 Cosmetic Compositions Comprising Extract from Mung-bean for Abirritating Skin
KR100472104B1 (en) * 2000-08-04 2005-03-07 네비온 주식회사 A composition for external application for skin whitening
KR100858630B1 (en) * 2007-04-27 2008-09-17 주식회사 코리아나화장품 Cosmetic composition for screening uv rays and preventing photo-aging comprising mung bean extract as active ingredients
KR100885478B1 (en) * 2007-05-30 2009-02-24 동의나라주식회사 Natural whitening composition having antioxidizing function

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100973942B1 (en) * 2008-02-27 2010-08-04 주식회사 코리아나화장품 Cosmetic Composition for Whitening of the Skin Comprising the Extract of Fermented Phaselous radiatus Seed

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR900013932A (en) * 1989-03-22 1990-10-22 박효철 Herbal Herbal Ingredients for Cosmetic Powders and Extracts
KR0161333B1 (en) * 1995-10-27 1998-12-01 유상옥 Cosmetic composition comprising extracts of mung bean(phaseolus aureus)
KR100272463B1 (en) * 1998-11-16 2000-11-15 박동기 Extract extracted from natural substance for using in cosmetics

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100472104B1 (en) * 2000-08-04 2005-03-07 네비온 주식회사 A composition for external application for skin whitening
KR20030000715A (en) * 2001-06-26 2003-01-06 장성환 Method wich mke powder for a beauty pack
KR20030042527A (en) * 2001-11-23 2003-06-02 주식회사 코리아나화장품 Cosmetic Compositions Comprising Extract from Mung-bean for Abirritating Skin
KR100858630B1 (en) * 2007-04-27 2008-09-17 주식회사 코리아나화장품 Cosmetic composition for screening uv rays and preventing photo-aging comprising mung bean extract as active ingredients
KR100885478B1 (en) * 2007-05-30 2009-02-24 동의나라주식회사 Natural whitening composition having antioxidizing function

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