KR101095609B1 - Cosmetic composition containing whitening Composition for whitening Skin - Google Patents

Abstract

The present invention relates to a cosmetic composition excellent in whitening effect, containing a whitening complex consisting of cheongung extract, Soruju extract, sewage extract, niacinamide, papain and nopal extract. More specifically, the whitening complex of the present invention has excellent activity inhibitory effect of α-MSH / SCF, gene inhibitory effect of TRP-1, TRP-2, tyrosinase activity inhibitory effect, melanosomal migration inhibitory effect, and keratin loss. It relates to a cosmetic composition which not only has an effect but also promotes skin turnover and is involved in all stages of melanin formation, and shows an excellent effect on whitening.

Whitening, complex, melanin, cosmetic

Description

Cosmetic composition for skin whitening containing a whitening complex {Cosmetic composition containing whitening Composition for whitening Skin}

The present invention relates to a cosmetic composition excellent in whitening effect by containing a whitening complex consisting of cheongung extract, soruja extract, sewage extract, niacinamide, papain and nopal extract. More specifically, the whitening complex of the present invention has excellent activity inhibitory effect of α-MSH / SCF, gene inhibitory effect of TRP-1, TRP-2, tyrosinase activity inhibitory effect, melanosomal migration inhibitory effect, and keratin loss. It relates to a cosmetic composition which not only has an effect but also promotes skin turnover and is involved in all stages of melanin formation, and shows an excellent effect on whitening.

The present invention relates to a cosmetic composition containing a whitening complex and excellent in whitening effect. More specifically, the present invention relates to a cosmetic composition containing a cheongung extract, a sorbus extract, sewage extract, niacinamide, papain and nopal extract, which shows excellent effect on whitening.

Skin is a very important tissue that has direct contact with the external environment, protects the human body and has biochemical and physical functions. This tissue is largely divided into three parts: the epidermis, the dermis, and the hypodermis.

Melanin is a pigment in the skin's epidermal layer that prevents skin damage caused by UV rays and protects the skin from external harmful factors such as trapping free radicals and free radicals generated in skin. .

On the other hand, melanin is made from melanocytes (melanocyte) present in the basal layer of the epidermis and migrate to epidermal cells called keratinocytes. There are no enzymes that break down melanin in vivo, but they are removed by falling off the skin as keratinocytes fall off the epidermis.

Melanosome endoplasmic reticulum in melanocytes contains three enzymes in melanogensis. First of all, tyrosine (L-3, tyrosinase) is caused by the most important enzyme called tyrosinase. It is converted to dopaquinone via 4-dihydroxyphenylalanine (DOPA) and becomes dopachrome by automatic oxidation from dopaquinone. Dopachrome again produces dihydroxy indole carboxylic acid (DHICA) by an enzyme called tyrosinase related protein-2 (TRP-2), known as dopachrome tautomerase. DHICA is then oxidized by tyrosinase related protein-1 (TRP-1), known as DHICA oxidase, to produce Indole-5,6-quinone-carboxylic acid, which then produces a dark brown eumelanin. . The melanin thus produced is transferred to the keratinocytes through the melanosomes, and after about 28 days of keratinization, the melanin comes out to the surface of the skin and disappears with the keratin.

 In skin tissues, to protect the body from ultraviolet rays, melanin is synthesized, but when the melanin is excessively synthesized and the release of skin is slowed down, pigmentation such as blemishes, freckles, spots, and mushrooms may occur, causing skin aging and damage. Done.

To stimulate melanin synthesis in the skin, keratinocytes secrete α-MSL (α-melanocyte-stimulating hormone) and SCF (Stem Cell Factor) when exposed to external stimuli such as ultraviolet rays. Cells secreted α-MSH and SCF stimulate the MSH receptors on the surface of melanocytes to induce melanin pigmentation. Second, mental stress and wounds increase hormone and cytokine secretion, affecting melanocytes, and third, pregnancy and disease.

And the cells of the stratum corneum have a cell turn over action that allows millions of dead keratinocytes to break away from the skin and be replaced with new keratinocytes. In general, the process of cell growth and differentiation is eliminated by the normal rate of the cell cycle, but the stratum corneum is not smoothly eliminated due to external environmental factors, stress, and skin aging. Eventually, if the aging keratin is not normally eliminated, the skin loses activity, remains on the skin surface, the stratum corneum becomes thick, darkens the skin tone, becomes dull, and the skin surface becomes rough.

Today, Asian women prefer white and clean skin like white jade and make it an important standard of beauty. Therefore, the development of whitening agent for treatment of abnormal skin pigmentation and satisfying beauty needs is being actively developed.

At present, in the development of a whitening agent for skin beauty, a method of reducing the generated melanin pigment by decolorization and a method of inhibiting the activity of tyrosinase, an enzyme that forms melanin pigment, are known. However, the whitening agent using tocopherol, vitamins, etc. which are used for reducing melanin pigment | dye is known that the decolorization effect of melanin pigment is insignificant.

Accordingly, attention has been paid to inhibitors that inhibit the production of melanin pigment by inhibiting the activity of tyrosinase. In the conventional cosmetic field, as a whitening component, for example, substances that inhibit tyrosinase enzyme activity such as kojic acid, arbutin, hydroquinone, vitamin-C (L-Ascorbic acid) and Their derivatives and various plant extracts have been used. However, the stability in the prescription system is poor, the color is degraded, or the use thereof is limited due to the occurrence of off-flavor, efficacy at the biological level, unclear effect and safety problems.

In addition, a method of obtaining a whitening effect by inhibiting only one process among the various stages of melanin synthesis has been generally studied, and a composition that inhibits the whole process of melanin synthesis is not known and thus has not yet exhibited an excellent whitening effect. to be.

In order to solve the above problems, the present inventors have divided the melanin pigment formation process into five steps to improve skin whitening.

As a result, the whitening complex formed from the extract of scorpion, Sorusup, sewage, niacinamide, papain and nopal extracts inhibits the activity of α-MSH / SCF secreted from keratinocytes by the first ultraviolet, second tyrosinase, Inhibits the expression of TRP-1 and TRP-2, inhibits the activity of the third tyrosinase, inhibits the movement of melanin (melanoma), fourth, promotes keratin exfoliation and The present invention has been completed by confirming that the activity can be increased to promote turnover of the skin and thus exhibit a very improved whitening effect.

Accordingly, it is an object of the present invention to provide a cosmetic composition in which all the steps of melanin production are blocked to maximize the whitening effect.

The present invention may include the following means to solve the above problems.

The cosmetic composition for skin whitening of the present invention is formed of a cheongung extract, Soru-ji extract, sewage extract, niacinamide, papain and nopal extract.

On the other hand, the total weight of the cosmetics is 0.001 ~ 5.0% by weight of the cheongung extract, 0.001 ~ 5.0% by weight of Soru-Jin extract, 0.001 ~ 10.0% by weight of sewage extract, 0.001 ~ 10.0% by weight of niacinamide, 0.01 ~ 0.01 of papain 1.0% by weight, nopal extract is characterized in that it contains 0.001 to 5.0% by weight.

The composition is characterized in that it has one formulation selected from the group consisting of softener, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder.

As described above through the following preparation examples and examples, the whole process of melanin production in the cosmetics containing the whitening complex of the cheongung extract, Sorujuk extract, sewage extract, niacinamide, papain and nopal extract of the active ingredient of the present invention By blocking the skin whitening cosmetic composition with excellent whitening effect was able to be completed.

Hereinafter, look at the present invention in detail

The present invention relates to a cosmetic composition for skin whitening containing a whitening complex as an active ingredient, wherein the whitening complex is formed of cheongung extract, Soru-Jung extract, sewage extract, niacinamide, papain, hapa extract.

The cosmetic composition of the present invention is characterized by inhibiting the activity of α-MSH / SCF, tyrosinase, TRP-1 and TRP-2, inhibiting the activity of tyrosinase, inhibiting melanin migration, exfoliation of keratin and promoting skin turnover. It relates to a skin whitening cosmetic composition.

The uterine extract used in the present invention is characterized by containing in an amount of 0.001 to 5.0% by weight based on the total weight of the composition. Preferably it is 0.1 to 2.0% by weight and when the content of the cheongung extract is less than 0.001% by weight it is difficult to expect the effect, when it exceeds 5.0% by weight does not appear to increase the apparent effect of increasing the content.

As an active ingredient of the cosmetic composition of the present invention, the extract is contained in an amount of 0.001 to 5.0% by weight based on the total weight of the composition. Preferably it is 0.1 to 2.0% by weight and when the content of the rubbing extract is less than 0.001% by weight it is difficult to expect the effect, when it exceeds 5.0% by weight does not appear to increase the apparent effect of increasing the content.

As an active ingredient of the cosmetic composition of the present invention, sewage extract is characterized by containing in an amount of 0.001 to 10.0% by weight based on the total weight of the composition. Preferably it is 0.5 to 5.0% by weight and when the content of the sewage extract is less than 0.001% by weight it is difficult to expect the effect, when it exceeds 10.0% by weight does not appear to increase the apparent effect of increasing the content.

As an active ingredient of the cosmetic composition of the present invention, niacinamide is characterized by containing in an amount of 0.001 to 5.0% by weight based on the total weight of the composition. Preferably it is 0.5 to 5.0% by weight and when the content of niacinamide is less than 0.001% by weight it is difficult to expect the effect, when it exceeds 10.0% by weight does not appear to increase the apparent effect of increasing the content.

As an active ingredient of the cosmetic composition of the present invention, papain is contained in an amount of 0.01 to 1.0% by weight based on the total weight of the composition. If the content of papain is less than 0.01% by weight, it is difficult to expect the effect, if it exceeds 1.0% by weight may cause skin irritation.

As an active ingredient of the cosmetic composition of the present invention, the nopal extract is characterized in that it is contained in an amount of 0.001 to 5.0% by weight based on the total weight of the composition. Preferably it is 0.1 to 2.0% by weight and the content of the nopal extract less than 0.001% by weight is difficult to expect the effect, when it exceeds 5.0% by weight does not appear to increase the apparent effect of increasing the content.

Cnidium officinale , an active ingredient in the present invention, is a perennial herb belonging to the mountain family, and is native to China, and its height is 30 to 60 cm and the stems stand straight. Roots are enlarged, beads, leaves are pinnate. Flowers are white and bloom in August to September, with 5 petals, 5 stamens and 1 pistil. Herbal medicine is used for medicinal roots, blood circulation, headache, pain, sedation, anti-ulcer, blood pressure control, antibacterial, blood is known to be effective, especially women's menstrual pain, postpartum abdominal pain is used.

Rumex crispus, an active ingredient of the present invention, is called a yangje-geun, sol-bang, a billiard, and the like, and is a perennial herb that grows and grows again when the stem is dried up in midsummer. Young leaves are eaten as herbs, and are said to be effective for ringworm, athlete's foot, and eczema. Since ancient times, it has been used for skin diseases such as amelioration, scabies and skin cancer, and it is also used for cystitis and colitis. It is called Yangje-geun when the roots are broken and dried in the autumn and dried in the sun.

Sewage (Polygonum multiflorum) is an active ingredient of the present invention is a vine medicinal plant that is known from China and has been cultivated for a long time. The height is 3 ~ 4m and the roots are extended to the side, often forming tubers. The reddish brown tuber is called Shouo in one shot. Shouo is famous as a herb that strengthens kidney function, enhances energy, darkens hair and makes people live longer without disease. Sewage is also a good medicine to boost the energy of the elderly. The kidney function is good, so that the hair does not whiten and the hair does not fall out. Chinese people regards sewage as the three major medicines with ginseng and wolfberry.

Niacinamide, an active ingredient of the present invention, is known as vitamin B3, and is also called pyridine-3-carboxyl or nicotinamide. Known effects include promoting the biosynthesis of ceramides, moisturizing and anti-inflammatory.

Papain, an active ingredient of the present invention, is a proteolytic enzyme found in fruit juices of papaya trees native to South America, and used in digestive and broiler meats.

Nopal (Opuntia ficus-indica L. var.saboten Makino), an active ingredient of the present invention, is a cactus cultivated around Jeju Island, and is called a palm cactus because its stem has a broad shape like a palm. . It is a perennial plant, reaching 2m in height, forming a fan-shaped section, and elongating. Yellow flowers of 2-3cm bloom in May-June. Calyx leaves, petals, stamens are numerous, there is one pistil, and flowers are alive with nerves and get stained. As flowers bloom, they bear fruit in November, and both fruits and stems are used for food.

Extract of the present invention can be prepared as follows.

Commercially purchased Cheongung, Soru-Jung, Sewao, and Nopal are purchased, washed with water, and dried in the shade. The dried Cheongung, Soru-Jung, Sewao, and Nopal are chopped and placed in an extraction container, and extracted for a predetermined time at an appropriate temperature in an appropriate amount of extraction solvent. The extract may be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. Preferably, water is effective, and the amount of the extraction solvent is 2 to 15 of the dry weight of the herbal medicine. Double it. The extraction method may be an extraction method such as water bath extraction, reflux cooling extraction, ultrasonic extraction, and the like, preferably reflux concentrated extraction at about 30 to 80 ℃, preferably about 50 to 60 ℃ as extraction reflux cooling method, extraction The time is effective for 5 to 20 hours, preferably 10 hours, and is characterized by being obtained by freeze drying.

The active ingredient of the present invention has the effect of inhibiting the activity of α-MSH / SCF, inhibiting the expression of tyrosinase, TRP-1 and TRP-2, inhibiting the activity of tyrosinase, inhibiting melanin migration, exfoliation of keratin and promoting skin turnover It can be effectively used as a whitening composition.

The skin whitening cosmetic composition of the present invention is not particularly limited in the formulation, the composition for skin whitening of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, Emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like, but are not limited thereto.

In addition to the active ingredients included in the cosmetic composition of the present invention cheongung extract, Soru-Jung extract, sewage extract, niacinamide, papain, hawa extract, including components commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers And conventional inclusions such as vitamins, pigments and flavorings.

Hereinafter, the present invention will be described in more detail with reference to the following examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited to these embodiments according to the gist of the present invention to those skilled in the art to which the present invention pertains. Will be self explanatory.

Preparation Example 1 Preparation of Cheongung Extract

50 g of commercially available Cheongung was ground to powder using a grinder, and then distilled water of 5 times the weight of Cheongung was added thereto, extracted using a reflux cooling extraction method, filtered through a 400 mesh filter cloth, and the filtrate was concentrated under reduced pressure. It was then freeze-dried to obtain 4.8 g of extract.

Preparation Example 2 Preparation of Soruja Extract

50 g of commercially available sorbet is ground into powder using a grinder, and then distilled water of 5 times the weight of sorbet is added thereto, and extracted using a reflux cooling extraction method, filtered through a 400 mesh filter cloth, and the filtrate is collected under reduced pressure. Concentrated and then lyophilized to obtain 4.3g of the extract.

Preparation Example 3 Preparation of Sewage Extract

50 g of commercially available sewage was ground into a powder using a grinder, and then distilled water of 5 times the weight of sewage was added thereto, extracted using a reflux cooling extraction method, filtered through a 400 mesh filter cloth, and the filtrate was concentrated under reduced pressure. It was then freeze-dried to obtain 5.1 g of the extract.

Preparation Example 4 Preparation of Nopal Extract

50 g of commercially available nopal was ground to a powder using a grinder, and then distilled water 5 times the weight of the nopal was added thereto, followed by extraction using a reflux cooling extraction method, filtered through a 400 mesh filter cloth, and the filtrate was concentrated under reduced pressure. It was then freeze-dried to remove 4.2 g of the extract.

Experimental Example 1 Melanin Synthesis Inhibitory Effect

The inhibitory effect of α-MSH / SCF on the extract prepared in Preparation Examples 1 to 4, niacinamide, and papain was performed as follows.

B16F1 melanoma cells were cultured in Dulbecco's modified Eagle's medium (DMEM) medium containing 10% fetal bovine serum, aliquoted 2.5 × 10 ⁴ cells per well in 24-well culture plates, and at 37 ° C., 5% CO ₂ conditions. Incubated. Here, the extracts of Preparation Examples 1 to 4, niacinamide and papain were treated with 0.05%, α-MSH (Sigma-Aldrich, USA) was added to 100 nM, and SCF (Vexxon, Korea) was added to α-MSH / The cells were divided into SCF-treated and non-treated groups, and then cultured for 2 days. After 2 days of culture, the cells were recovered, and the recovered cells were reacted at 90 ° C. for 1 hour by adding 1N NaOH containing 10% DMSO to be sufficiently dissolved in melanin, and then, at a wavelength of 405 nm using an ELISA reader. Absorbance was measured. The experimental results are shown in Table 1.

* Melanin production inhibitory effect sample Melanin production (%) Untreated group α-MSH treatment group SCF treatment group Control group 100.0 100.0 100.0 Preparation Example 1 88.3 33.4 42.5 Production Example 2 85.1 41.7 31.6 Production Example 3 46.1 50.6 51.4 Preparation Example 4 94.5 95.3 97.3 Niacinamide 96.8 96.8 95.6 Papain 99.0 99.0 98.1

As can be seen from the results of Table 1, it can be seen that the melanin production inhibitory effect is shown in Preparation Examples 1, 2, and 3. In the case of Cheongung extract of Preparation Example 1, the best melanin production inhibitory effect was obtained in α-MSH treatment, and the extract of Soru-Jin extract of Preparation Example 2 had the best melanin production inhibitory effect in SCF treatment. This showed the best melanin production inhibitory effect. In addition, in the sewage extract of Preparation Example 3, all of the α-MSH / SCF treatment group showed a melanin production inhibitory effect to some extent.

Experimental Example 2 Gene Expression Inhibition Effect

In order to determine the effects of tyrosinase, TRP-1 and TRP-2 gene expression on the extracts prepared in Preparation Examples 1 to 4, niacinamide, and papain, were performed as follows.

B16F1 melanoma cells were cultured in Dulbecco's modified Eagle's medium (DMEM) medium containing 10% fetal bovine serum, aliquots of 1.6 × 10 ⁶ / dish cells, and cultured under 37 ° C., 5% CO ₂ conditions. Here, the extracts of Preparation Examples 1 to 4, niacinamide and papain were treated with 0.05%, and α-MSH (Sigma-Aldrich, USA) was treated with 100 nM. After culturing for 2 days, the culture medium was removed, and then 1 ml of Trizol (Trizol, invitrogen, USA) was added thereto, followed by RNA isolation by Invitrogen. Reverse transcription-polymerase chain reaction was performed to make cDNA, and reverse transcription-polymerization reaction was performed using an all-in-one RT-PCR kit (SuperBio, Korea). The conditions were reported in Ho Min Kim, et al., Korean J. Oriental Physiology & Pathology 22 (6): 1444 ~ 1448, 2008, and quantified by electrophoresis on 2% agarose gel. The correction was made with actin and the results are shown in Table 2 below.

sample Gene expression level Tyrosinase TRP-1 TRP-2 α-MSH 100.0 100.0 100.0 Preparation Example 1 + α-MSH 94.3 96.7 97.7 Preparation Example 2 + α-MSH 92.1 94.8 92.4 Preparation Example 3 + α-MSH 43.5 51.1 50.0 Preparation Example 4 + α-MSH 99.6 97.4 97.5 Niacinamide + α-MSH 93.4 94.3 94.8 Papain + α-MSH 99.9 99.9 99.4

As can be seen from the results of Table 2, it was found that the tyrosinase, TRP-1, and TRP-2 gene expression inhibitory effects were obtained from the sewage extract of Preparation Example 3.

Experimental Example 3 Measurement of Tyrosinase Activity Inhibition

The extracts prepared in Preparation Examples 1 to 4 and niacinamide, papain were carried out as follows to determine the effect on tyrosinase enzyme activity.

210 μl of 0.1 M phosphate buffer (pH 6.8) was added to the 96 well plate, and the extract prepared in Preparation Examples 1-4 at the final concentration of 200 μg / ml, and 30 μl of niacinamide, papain or distilled water were added. 20 μl of mushroom-derived tyrosinase is then added. After addition of 40 μl of 5 mM L-DOPA as a substrate, the reaction was carried out at 37 ° C. for 10 minutes, and then the absorbance was measured at a wavelength of 475 nm using an ELISA reader. The control group was distilled water, and the results are shown in Table 3 below.

sample Tyrosinase activity (%) Control group 100.0 Preparation Example 1 94.5 Production Example 2 96.4 Production Example 3 50.1 Preparation Example 4 95.1 Niacinamide 94.0 Papain 99.8

As can be seen from the results of Table 3, the activity of tyrosinase enzyme in the sewage extract of Preparation Example 3 was found to be inhibited by 49.1% compared to the control.

Experimental Example 4 Melanosome Movement Inhibition Effect Measurement

The extracts prepared in Preparation Examples 1 to 4 and niacinamide, papain were carried out as follows to determine the melanoma migration inhibitory effect.

Keratinocytes and melanocytes were isolated from the neonatal foreskin. Specifically, the epidermis was separated from the foreskin tissue by treatment with 0.25% trypsin, and then 7.5 mg / ml bovine pituitary extract, 0.1 mg / ml human epidermal growth factor (hEGF), 0.5 mg / ml hydrocortisone, 50 Cells were isolated by vortexing with KGM (Clonetics) medium containing mg / ml gentamicine, 50 mg / ml amphotericin and 5 mg / ml insulin, incubated in a T75 flask and keratinized. Cells and melanocytes were obtained respectively. At this time, the cultured melanocytes were stained with carboxy fluorescein diacetate (CFDA) to analyze the melanosomes. For experiments, keratinocytes and melanocytes were co-cultured in coculture medium (KGM + (KGM-TPA-hydrocortisone)). Then, 0.05% of the samples were treated with the cells co-cultured at 12 hour intervals for 6 days. And nothing was added as a control. The treated cells were cultured with mouse monoclonal antibodies against anticytokeratin (Zymed Laboratories, USA) to separate keratinocytes from fluorescence activated cell sorting (FACS), and then anti-mouse bound with phycoerythrin. IgG (Burlingame, United States) and the cultured cells were reacted for 30 minutes at 37 ℃. Cells cultured with a scraper were then removed from the flask and FACS analyzed using EPICSXL flow cytometer (Coulter Cytometry, Coulter Corp., United States). At this time, in the FACS analysis, keratinocytes reacting with phycoerythrin at 575 nm were analyzed, and melanosomes of keratinocytes were confirmed by measuring CFDA at 525 nm. The results obtained in the coculture were analyzed using Coulter XL software (Coulter Corp.) and the results are shown in Table 4 below.

sample Melanosome migration inhibition rate (%) Control group 0.0 Preparation Example 1 0.9 Production Example 2 1.1 Production Example 3 1.3 Preparation Example 4 0.7 Niacinamide 62.1 Papain 0.0

As can be seen from the results in Table 4, it was found that niacin amide has excellent melanocyte migration inhibitory effect.

Experimental Example 5 Trypsin Activity Promoting Effect

In order to determine the effect of the proteolytic enzyme activity on the extract and niacinamide prepared in Preparation Examples 1 to 4 were carried out as follows.

BAPNA (N-benzoyl-D, L-arginine-p-nitrid hydrochloride) acts as a substrate for trypsin and when hydrolyzed by trypsin, p-nitroalanine is produced which exhibits maximum absorbance at 410 nm. Using this method, the following experiment was performed to determine the trypsin activity promoting effect on the extracts and niacinamide of Preparation Examples 1-4.

100 μl each of the extract prepared in Preparation Examples 1 to 4 and the concentration of niacinamide at 0.5% were taken, respectively, and 50 mM Tris / HCl (pH 8.2) containing 100 μl of 60 μg / ml trypsin solution and 50 mM CaCl 2 was used. After incubation for 10 minutes at 37 ° C., 600 μl of 400 μg / ml BAPNA solution was added thereto, followed by incubation for 2 minutes at 37 ° C. for 30 minutes, and 30% acetic acid was added to terminate the reaction. . Distilled water was used as a control and the results are shown in Table 5 below.

Trypsin activity promoting effect sample Trypsin activity promoting effect Control group 0.0 Preparation Example 1 1.1 Production Example 2 0.8 Production Example 3 0.9 Preparation Example 4 41.8 Niacinamide 0.9

As can be seen from the results of Table 5, it could be seen that there is a trypsin activity promoting effect in the nopal extract of Preparation Example 4.

[Production Example 5]

A cosmetic composition was prepared containing a whitening complex of cheongung extract, sorbus extract, sewage extract, niacinamide, papain and nopal extract.

ingredient Example 1 (% by weight) Example 2 (% by weight) Example 3 (% by weight) Example 4 (% by weight) Example 5 (% by weight) Example 6 (% by weight) Example 7 (% by weight) Comparative Example (wt%) Celestial extract 1.0 6.0 Sorousum Extract 1.0 6.0 Sewao Extract 1.0 6.0 Niacinamide 1.0 6.0 Papain 1.0 6.0 Nopal extract 1.0 6.0 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Liquid paraffin 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Polysorbate 60 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Sorbitan sesquirol
Olate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Squalane 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Beeswax 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Triethanolamine 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Propylene glycol 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Carboxyl Vinyl Polymer 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 incense a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance Balance Balance Balance Balance system 100 100 100 100 100 100 100 100

Experimental Example 6 Whitening Effect

In order to measure the whitening effect of the present invention, healthy women in their 30s and 40s were divided into 8 groups of 10 to 10 people each of Examples 1 to 7 and Comparative Examples to be applied twice a day for 12 weeks. The black and white degree of skin was apply | coated and the effect was judged using the color difference meter (Minolta CR2002). L *, a *, b * color system is used to display color. In this experiment, L * value (brightness) was mainly used as an index. L * values range from black (L * = 0) to white (L * = 100), and ΔL * after 12 weeks was obtained and shown in Table 7 below.

Test substance ΔL * Example 1 44.5 Example 2 8.3 Example 3 7.9 Example 4 9.4 Example 5 8.0 Example 6 7.9 Example 7 7.4 Comparative example 1.12

As can be seen in Table 7, it was shown that the whitening effect in Examples 1 to 7 containing the composition of the present invention, in particular, the most excellent whitening effect in Example 1 containing all the active ingredients of the present invention can be seen. there was.

 Hereinafter, as an example of the cosmetic formulation of the present invention, the softening lotion, essence, shampoo, body cleanser, etc. containing the whitening complexes of the active ingredient of the present invention, Cheongung extract, Soruch extract, sewage extract, niacinamide, papain and nopal extract However, the formulation of the cosmetic of the present invention is not necessarily limited thereto.

Formulation Example 1 Flexible Cosmetics

Softening water containing the active ingredient was prepared according to a conventional method.

Raw material name Content (% by weight) Celestial extract 2.0 Sorousum Extract 2.0 Sewao Extract 2.0 Niacinamide 2.0 Papain 0.1 Nopal extract 2.0 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 2 Essence

An essence containing the active ingredient was prepared according to a conventional method.

Raw material name Content (% by weight) Celestial extract 2.0 Sorousum Extract 2.0 Sewao Extract 2.0 Niacinamide 2.0 Papain 0.1 Nopal extract 2.0 Cetostearyl alcohol 1.0 Glyceryl Monostearate 0.8 Sorbitan monostearate 0.3 Porofilparaben 0.1 Polysorbate 60 1.0 Mineral oil 5.0 Pyromethicone 3.0 Dimethicone 0.5 Allantoin 0.1 glycerin 5.0 Alcohol 2 Propylene glycol 3.0 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 3 Shampoo

Shampoo containing the active ingredient was prepared according to a conventional method.

Raw material name Content (% by weight) Celestial extract 2.0 Sorousum Extract 2.0 Sewao Extract 2.0 Niacinamide 2.0 Papain 0.1 Nopal extract 2.0 Alkyl sulfate (30%) 25.0 Alkyl ether sulfate (30%) 20.0 Alkylamidobetaine (30%) 5.0 Propylene glycol 3.0 Lauramid 4.0 EDTA-2Na 0.1 Polyquaternium-10 0.05 NaCl 0.1 Citric acid 2.4 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 4 Body Cleanser

A body cleanser containing the active ingredient was prepared according to a conventional method.

Raw material name Content (% by weight) Celestial extract 2.0 Sorousum Extract 2.0 Sewao Extract 2.0 Niacinamide 2.0 Papain 0.1 Nopal extract 2.0 Alkyl ether saccharide (30%) 30.0 Alkyl ether sulfosuccinate (30%) 20.0 Alkylamidobetaine (30%) 5.0 Propylene glycol 5.0 Betaine 5.0 Lauramiddiei 4.0 Cellulose gum 0.05 Hydrolyzed protein 0.1 Citric acid a very small amount NaCl a very small amount incense a very small amount antiseptic a very small amount Purified water Balance system 100

Claims (3)

A cosmetic composition for skin whitening containing a whitening complex formed from a cheongung extract, an extract of sol, extract of sewage, niacinamide, papain and nopal as an active ingredient. According to claim 1, wherein the total weight of the cosmetic extract is 0.001 to 5.0% by weight, Soruch extract is 0.001 to 5.0% by weight, sewage extract is 0.001 to 10.0% by weight, niacinamide is 0.001 to 10.0% by weight, papain Silver 0.01 ~ 1.0% by weight, Nopal extract is a cosmetic composition for skin whitening containing a whitening complex as an active ingredient, characterized in that it contains 0.001 to 5.0% by weight. The composition of claim 1, wherein the composition comprises one formulation selected from the group consisting of softener, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder. Cosmetic composition for skin whitening containing a whitening complex as an active ingredient having.