KR100681700B1 - Cosmetic composition for skin whitening comprising senkyunolide a and gluconic acid as active ingredients - Google Patents

Abstract

A cosmetic composition is provided to show improved skin whitening effect without skin irritation by comprising senkyunolide A having melanocyte stimulating hormone activity inhibition effect and gluconic acid capable of removing melanin accumulated at keratin. The skin whitening cosmetic composition comprises senkyunolide A(3-butyl-6,7-dihydrophthalide) and gluconic acid as effective ingredients. The senkyunolide A is isolated from Cnidium officinale or chemically synthesized. Regarding the total weight of the cosmetic composition, 0.00001-20.0 wt.% of the senkyunolide A and 0.001-10.0 wt.% of the gluconic acid are contained.

Description

Cosmetic Composition for Skin Whitening Comprising Senkyunolide A and Gluconic Acid as Active Ingredients}

The present invention relates to a cosmetic composition for skin whitening.

The pigments involved in human skin color development include melanin, carotene and hemoglobin (oxidized hemoglobin, reduced hemoglobin), among which melanin is the most important factor in determining the color of the skin, depending on the amount, nature and distribution of the skin. This is determined.

The main cause of darkening of human skin is due to the synthesis of this melanin. That is, when the skin is exposed to ultraviolet rays, melanin production promoting factors are secreted from keratinocytes, which are skin epidermal cells, and melanocytes, a kind of skin cells, are stimulated by these factors to synthesize melanin, and melanin is synthesized on the skin surface. Is released and the skin epidermis becomes black.

The mechanism by which melanin is synthesized in melanocytes is as follows. That is, dopaquinone is produced by the action of an enzyme called tyrosinase using the tyrosine in the cell as a substrate, and 5,6-dihydroxyindole (DHI), a monomer of melanin, is passed through dopachrome by a continuous oxidation reaction from dopaquinone. ) And 5,6-dihydroxyindole-2-carboxylic acid (DHICA) are produced, and they are polymerized with each other to finally form a melanin copolymer.

Since melanin is a very stable compound that does not decompose even at high temperatures, inhibiting melanin production by inhibiting some reactions in the melanogenesis process is the most common method of preventing the skin from turning black. Conventional melanin suppression methods are chelates such as kojic acid, substrate analogs such as arbutin, plant extracts such as upper limb extract and licorice extract.

However, these whitening raw materials have a disadvantage in that there is little practical whitening effect because there is no ability to remove already formed melanin pigment. In order to compensate for the above disadvantages, skin exfoliants such as lactic acid having a melanin pigment removal function are used in addition to the whitening cosmetic composition in the art. However, lactic acid exhibits a high activity in strong acidic conditions, so the effect is very low in general cosmetic compositions, and there is a strong irritation problem.

Therefore, the melanin pigment removal function is superior to the existing skin whitening cosmetics, the development of skin whitening cosmetics without skin irritation is urgently required in the art.

Throughout this specification many patent documents are referenced and their citations are indicated. The disclosure of the cited patent documents is hereby incorporated by reference in their entirety, and the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

Accordingly, the present inventors have made diligent efforts to solve the problem that conventional tyrosinase inhibitors or substances that inhibit the activity of melanocyte stimulating hormones reduce the production of new melanin but do not remove the already produced pigments in a short time. In addition to the fresh quinolide A, which has an excellent effect of inhibiting the activity of melanocyte stimulating hormone, gluconic acid, which has almost no stimulus and excellent exfoliation effect, is applied to cosmetics, compared to lactic acid, a conventional keratin remover. The present invention has been completed by knowing that a superior whitening effect can be obtained through melanin production inhibition and melanin removal effect accumulated in keratin.

Accordingly, it is an object of the present invention to provide a cosmetic composition for skin whitening, which contains Sancunolide A and gluconic acid as an active ingredient.

Other objects and advantages of the present invention will become apparent from the following detailed description and claims.

The present invention provides a cosmetic composition for skin whitening, which contains Sancunolide A and gluconic acid as an active ingredient.

Sankyunolide A (3-butyl-6,7-dihydrophthalide), one of the active ingredients of the cosmetic composition of the present invention, inhibits melanocyte stimulating hormone activity, inhibits melanin production, and inhibits the production of tyrosinase. It is the substance which shows the whitening effect through the inhibitory effect (refer: Experimental example 1 and 2). Excellent skin lightening effect of the skin composition for skin whitening including Sancu Noride A is disclosed in Korean Patent No. 504408.

The Sancunolide A of the present invention is isolated from the natural arch of the uterus. The horoscope is a perennial herb belonging to the Apiaceae, Cnidium officinale and Ligusticum chuanxiong may be used.

The uterus includes various types of phthalides (eg, ligustilide, sanquinolide A, and sanquinolide H, etc.), among which the novel quinolide A of the present invention inhibits the activity of melanocyte stimulating hormone. There is a use, thereby inhibiting the biosynthesis of tyrosinase itself, thereby inhibiting the activity of tyrosinase, showing a skin whitening effect superior to the conventional whitening agent. In addition, the present invention, the quinolide A can be used as a superior whitening material because it exhibits the above-mentioned activity at a lower concentration than the cheongung extract itself.

In the present invention, the uterus used for extracting the quinomolide A can be used for various organs, tissues (roots, leaves, flowers, stems, fruits, seeds, etc.), and most preferably, the root of the uterus is used. .

Preferably, the fresh quinolide A of the present invention is isolated from the horoscope extract. The extract may be prepared by various extraction solvents such as (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (c) the lower alcohol and water Mixed solvent, (d) acetone, (e) ethyl acetate, (f) chloroform, (g) 1,3-butylene glycol, and (h) butyl acetate can be obtained from Cheongung. More preferably, the astronomical extract of the present invention is obtained using a hydrous lower alcohol, most preferably ethanol. On the other hand, it will be apparent to those skilled in the art that the astronomical extract of the present invention can be obtained in addition to the above-described extraction solvent, as well as the astronomical extract having substantially the same effect using other extraction solvent.

On the other hand, the fresh quinolide A of the present invention can be isolated and purified from the cheongung extract by a known method. For example, the Sancunolide A can be prepared by various chromatography such as gas chromatography (GC), high speed gas chromatography (HSGC), liquid chromatography (LC), high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). It can be separated and purified by such a method.

In addition, in addition to the isolated from the cheongoung extract of the present invention, the quinolide A of the present invention can also be used chemically synthesized.

Gluconic acid used as another active ingredient in the cosmetic composition of the present invention is one of the components of the alpha hydroxy acid (AHA) series produced by glucose fermentation, while the components of the alpha hydroxy series are skin aging. Efficacy in removing excessively accumulated aging keratin, the removal of keratin promotes the regeneration of the skin, as a result can be used to improve skin elasticity and skin moisturizing. The most widely used AHAs are fermented milk or lactic acid obtained by enzymatic reaction (Lactic acid, MW = 90.08), citric acid (MW = 210.14) contained in large quantities in fruits such as lemon or orange, apples, grapes and strawberries. And a large amount of malic acid (Malic acid, MW = 130.98) and tartaric acid (Tartaric acid, MW = 150.09) obtained from grapes.

Gluconic acid, which is included as an active ingredient in the cosmetic composition of the present invention, is an environmentally friendly substance with excellent biological resolution, and effectively separates the melanin pigment deposited on the skin. There is an advantage that does not irritate.

Gluconic acid of the present invention exhibits an excellent whitening effect through a complementary action by being used with Sancunolide A having excellent efficacy in inhibiting the activity of melanocyte stimulating hormone. The excellent whitening effect is that gluconic acid removes the dead skin of the skin to remove the melanin pigment existing in the existing stratum corneum, and the fresh quinoide A component extracted from the natural cheongung extract is penetrated into the skin to inhibit melanin production. Synergy occurs.

According to a preferred embodiment of the present invention, in the cosmetic composition of the present invention, the contents of the fresh quinolide A and the gluconic acid as active ingredients are 0.00001 to 20.0% by weight and 0.001 to 10.0% by weight, respectively, based on the total weight of the cosmetic composition. More preferably, they are 0.0001-10.0 weight% and 0.05-5.0 weight%. The skin whitening effect is less likely to occur when the contents of Sancu-Noride A and Gluconic acid are less than 0.00001% and 0.001% by weight, respectively, and when it exceeds 20.0% and 10.0% by weight, respectively, it is highly likely to cause skin irritation. It can also affect stabilization.

The cosmetic composition of the present invention may further include a whitening component in addition to the raw quinolide A and gluconic acid as an active ingredient exhibiting a whitening effect, but also shows an excellent whitening effect with only the raw cunolide A and gluconic acid.

The components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions in addition to raw quinolide A and gluconic acid as active ingredients, and include, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments and perfumes. Conventional adjuvants, and carriers.

The whitening composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing agents, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it may be prepared in the form of softening cream, converging cream, nourishing cream, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular, in the case of spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Preparation Example 1 Isolation and Purification of Sanquinolide A

3 kg of dried Cheongung root powder purchased from Gyeongdong Market, Seoul, Korea, was deposited in 18 liters of ethanol for 3 to 5 days, filtered using Whatman filter paper No. 5, and then completely reduced to a rotary vacuum evaporator equipped with a cooling condenser. Dried. The dried powder was partitioned into a mixture containing ethyl acetate and water, and the ethyl acetate layer was evaporated under reduced pressure again. The material thus obtained was partitioned through an open silica column (normal hexane: ethyl acetate = 3: 1) with an R _f value of 0.5 in TLC to obtain 30.1 g under reduced pressure. Here, the value of 0.5 of the R _f are the results obtained by the experimental values of the R _f of the substance having the activity using a preparation (prep) TLC previously. To purify the material obtained by distillation under reduced pressure, the compound was separated using Prep LC, distilled under reduced pressure, and the residual solvent was removed using a vacuum pump to obtain 3.1 g of Sanquinolide A:

Mass of extract: 192.12; ms (m / z (%)) 192 (17.19), 163 (1.91), 135 (3.57), 107 (100); ¹ HNMR 6.21 (dt), 5.91 (dt), 4.93 (dd) ) 2.47-1.39 (m), 0.9 (t); IR 2932, 2872, 1747, 1241 cm ⁻¹ ; UV (207, 278 nm).

Experimental Example  One: Saint-Quinoide  Inhibitory Effect of A on Melanin Production

The inhibitory effect of melanin production by the inhibition of melanocyte stimulating hormone activity of the raw quinolide A prepared in Preparation Example 1 was tested as follows.

Melanosite cells were purchased from a mouse derived B-16 melanoma cell line (ATCC CRL 6323). Melanoma cell lines were inoculated in DMEM medium containing 4.5 g / l glucose, 10% serum and 1% antibiotic and incubated at 37 ° C. in a 50 ml T-flask. After culturing for 24 hours under 5% CO ₂ condition, the cells were separated by treatment with 0.05% trypsin containing 0.02% EDTA, followed by culturing to 5 × 10 ⁴ cells / plate on φ100 plates. After 24 hours of incubation, 100 μM of melanocyte stimulating hormone (α-MSH, Sigma, United States of America) was added to each plate, followed by addition of the fresh quinolide A at concentrations of 5, 10, 20 and 50 μg / ml, respectively. After 5 days of culture, cells in each plate were obtained to quantify the amount of melanin. First, 5% trichloroacetate (TCA) was treated, stirred and centrifuged to wash the precipitated melanin with phosphate saline buffer, and then dissolved by treating with 1N NaOH in the precipitated melanin and measuring the absorbance at 475 nm. The amount of was quantified. Melanin concentrations were determined from standard concentration curves of synthetic melanin (Sigma, USA) and the inhibition rate of melanin production of each extract was determined. The results of this experiment are shown in Table 1 below.

Concentration of Sancunolide A (μg / ml) Melanin production rate (%) 0 ^* 100.0 0 ^** 175.8 5 ^** 160.6 10 ^** 121.3 20 ^** 105.5 50 ^** 98.7 ^* Melanosite stimulating hormone no treatment ^** Melanosite stimulating hormone 100μM treatment

As shown in Table 1, the fresh quinolide A of the present invention concentration-dependently reduces the melanin production rate in melanocytes treated with melanocyte stimulating hormone, and melanocyte stimulation when 50 μg / ml or more was treated. The melanin production rate was lower than the melanin production rate (100.0%) in the hormone-treated melanocytes. Therefore, it can be seen that the raw quinolide A of the present invention has an effect of inhibiting melanin production by inhibiting melanocyte stimulating hormone activity excellently.

Experimental Example 2: Confirmation of the inhibition of tyrosinase production of the Sancunoride A

Melanocite cells were cultured in the same manner as in Experimental Example 1, and 5, 10, 20, and 50 µg / ml of the fresh quinolide A prepared in Preparation Example 1 were added to the plates, respectively. Cells were then harvested after 3 days of culture. The harvested cells were washed twice with sodium phosphate buffer, treated with 0.1% Triton X-100, and reacted for 30 seconds using an ultrasonic crusher. The reaction solution was separated from the cell debris and the supernatant by using a high speed centrifuge (12,000 rpm, 30 minutes, 4 ° C). The separated supernatant was separated by protein using electrophoresis and reacted with a reaction solution containing 0.2% dopa solution for about 8 hours, and then the amount of tyrosinase produced in each gel was confirmed. The results of the experiment are shown in Table 2 below.

Concentration of Sancunolide A (μg / ml) Tyrosinase band strength 0 ^* 100 0 ^** 205 5 ^** 185 10 ^** 153 20 ^** 132 50 ^** 101 ^* Melanosite stimulating hormone no treatment ^** Melanosite stimulating hormone 100μM treatment

As shown in Table 2, the present embodiment of the present invention, quinolide A, reduces the concentration of tyrosinase production in melanocytes treated with melanocyte stimulating hormone, and melano when 50 μg / ml or more is treated. The band intensity was similar to that of tyrosinase band in melanocytes which were not treated with the site stimulating hormone. Therefore, the present invention, the quinolide A inhibits the activity of melanocyte stimulating hormone to inhibit the biosynthesis of tyrosinase itself, thereby suppressing melanin production better than the inhibition of melanin production by the inhibitory effect of tyrosinase activity of the conventional whitening agent. It can be seen that the effect.

Experimental Example 3: Exfoliation Effect of Gluconic Acid

In order to measure the exfoliation effect of gluconic acid (Envitech, Inc., Korea), ten Korean volunteers with bright skin color between 20 and 33 years old were tested. Instrabronze containing a dihydroxyacetone (DHA) component was used to measure the exfoliation pattern. Intrabronze stains skin keratin by converting free amino acids in the keratin protein into a brown substance called melanoidin. The experimental method is as follows. First, three square zones of width x length of 1 cm were formed on the subject's arms, and 10 µl of 10% Intrabronze was applied twice a day to sufficiently dye the skin brown. After staining, the initial L (brightness) value of each zone was measured using the Minolta CR 300 measuring device. Distilled water, 2% lactic acid and 2% gluconic acid (45% solution), which were each exfoliated, were applied twice a day to the stained test sites, and L values were measured on days 3, 6, 8 and 10. The results are shown in Table 3.

sample Distilled water 2% lactic acid 2% Gluconic Acid (45% solution) ΔL (3 days) 0.8 1.3 1.5 ΔL (6 days) 2.0 2.2 2.6 ΔL (8 days) 2.6 3.0 4.0 ΔL (10 days) 3.6 4.6 6.3

As can be seen from the experimental results of the exfoliation effect of Table 3, gluconic acid was excellent in exfoliation effect than lactic acid used as a whitening raw material as a keratin exfoliant as well as distilled water as a control.

Hereinafter, examples of the formulation of the present invention include a softening lotion, a convergent lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence and a pack, but the formulation of a cosmetic including the composition of the present invention is not limited thereto.

Formulation Example 1 Softener (Skin Lotion)

Softening water containing the sanctuide A and 45% gluconic acid solution as in Table 4 below was prepared according to a conventional method.

ingredient Content (Unit: wt%) Saint-Quinoride A 1.00 45% Gluconic Acid Solution 0.50 glycerin 5.00 1,3-butylene glycol 3.00 PEG 150 1.00 Allantoin 0.10 DL-panthenol 0.30 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 5.00 ethanol 10.0 Octyldodeceth-16 0.20 Polysorbate 20 0.20 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Formulation Example 2: Converging Cosmetic Water

As shown in Table 5 below, astringent lotion including Sancunolide A and 45% gluconic acid solution was prepared according to a conventional method.

ingredient Content (Unit: wt%) Saint-Quinoride A 1.00 45% Gluconic Acid Solution 0.50 glycerin 2.00 1,3-butylene glycol 2.00 Allantoin 0.20 DL-panthenol 0.20 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 3.00 ethanol 15.00 Polysorbate 20 0.30 Witch hazel extract 2.00 Citric acid a very small amount Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Formulation Example 3: Nutritional Cosmetics

As shown in Table 6, a nourishing lotion including fresh quinolide A and 45% gluconic acid solution was prepared according to a conventional method.

ingredient Content (Unit: wt%) Saint-Quinoride A 1.50 45% Gluconic Acid Solution 1.00 Stearyl alcohol 1.50 lanolin 1.50 Polysorbate 60 1.30 Sorbitan stearate 0.50 Cured Vegetable Oil 1.00 Mineral oil 5.00 Squalane 3.00 Trioctanoine 2.00 Dimethicone 0.80 Tocopherol Acetate 0.50 Carboxy Vinyl Polymer 0.12 glycerin 5.00 1,3-butylene glycol 3.00 Sodium hyaluronate 5.00 Triethanolamine 0.12 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Formulation Example 4: Nutrition Cream

A nutritive cream containing Sancunolide A and 45% Gluconic Acid solution was prepared according to a conventional method as shown in Table 7 below.

ingredient Content (Unit: wt%) Saint-Quinoride A 1.50 45% Gluconic Acid Solution 1.00 Lipophilic monostearate 2.20 Stearic acid 1.50 Beeswax 1.00 Polysorbate 60 1.50 Sorbitan stearate 0.60 Cured Vegetable Oil 1.00 Squalane 3.00 Mineral oil 5.00 Trioctanoine 5.00 Dimethicone 1.00 Sodium magnesium silicate 0.10 glycerin 5.00 Betaine 3.00 Triethanolamine 1.00 Sodium hyaluronate 4.00 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Formulation example 5: Massage cream

A massage cream including fresh quinolide A and 45% gluconic acid solution was prepared according to a conventional method as shown in Table 8 below.

ingredient Content (Unit: wt%) Saint-Quinoride A 1.00 45% Gluconic Acid Solution 0.50 Lipophilic monostearate 1.50 Stearyl alcohol 1.50 Stearic acid 1.00 Polysorbate 60 1.50 Sorbitan stearate 0.60 Isostearyl isostearate 5.00 Squalane 5.00 Mineral oil 35.00 Dimethicone 0.50 Hydroxyethyl Cellulose 0.12 glycerin 6.00 Triethanolamine 0.70 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum Pigment

Formulation Example 6: Essence

As shown in Table 9 below, the essence comprising the fresh curide A and 45% gluconic acid solution was prepared according to a conventional method.

ingredient Content (Unit: wt%) Saint-Quinoride A 1.50 45% Gluconic Acid Solution 1.00 glycerin 10.00 Betaine 5.00 PEG-150 2.00 Allantoin 0.10 DL-panthenol 0.30 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.10 Sodium hyaluronate 8.00 Carboxy Vinyl Polymer 0.20 Triethanolamine 0.18 Octyldodecanol 0.30 Octyldodeceth-16 0.40 ethanol 6.00 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Formulation example 7: Pack

As shown in Table 10 below, a pack including Sancunolide A and 45% gluconic acid solution was prepared according to a conventional method.

ingredient Content (unit: weight%) Saint-Quinoride A 1.00 45% Gluconic Acid Solution 0.50 Polyvinyl alcohol 15.00 Cellulose gum 0.15 glycerin 3.00 PEG-150 2.00 Saclodextrin 0.15 DL-panthenol 0.40 Allantoin 0.10 Monomethylammonium Glycyric Acid 0.30 Nicotinamide 0.50 ethanol 6.00 PEG-40 Cured Castor Oil 0.30 Preservative, fragrance, coloring a very small amount Distilled water Remaining amount Sum 100

Experimental Example 4 Measurement of Whitening Effect

Nutritional cream of Formulation Example 4 and Comparative Example 1 and 30 according to the composition shown in Table 11 to determine the whitening effect for the cosmetic composition of the present invention in 30 Korean female volunteers aged 19-40 with dark skin After preparing the nutrition cream of 2 was measured the whitening.

ingredient Content (Unit: wt%) Formulation Example 4 Comparative Example 1 Comparative Example 2 Saint-Quinoride A 1.50 1.50 1.50 45% Gluconic Acid Solution 1.00 - - Lactic acid - - 1.00 Lipophilic monostearate 2.20 2.20 2.20 Stearic acid 1.50 1.50 1.50 Beeswax 1.00 1.00 1.00 Polysorbate 60 1.50 1.50 1.50 Sorbitan stearate 0.60 0.60 0.60 Cured Vegetable Oil 1.00 1.00 1.00 Squalane 3.00 3.00 3.00 Mineral oil 5.00 5.00 5.00 Trioctanoine 5.00 5.00 5.00 Dimethicone 1.00 1.00 1.00 Sodium magnesium silicate 0.10 0.10 0.10 glycerin 5.00 5.00 5.00 Betaine 3.00 3.00 3.00 Triethanolamine 1.00 1.00 1.00 Sodium hyaluronate 4.00 4.00 4.00 Preservative, fragrance, coloring a very small amount a very small amount a very small amount Distilled water Remaining amount Remaining amount Remaining amount Sum 100 100 100

As for the measurement site | part, four places of 1 * 1cm <2> were displayed on the arm, and each sample (formulation example 4 and comparative examples 1 and 2) was apply | coated to three parts continuously, and one place was untreated and used as a control. Subsequently, the whitening degree (ΔL value) was measured at two week intervals for two months using a colorimeter (Minolta CR 300) as a measuring device. The results are shown in Table 12.

division Formulation Example 4 Comparative Example 1 Comparative Example 2 Control ΔL value 4.15 2.80 3.55 0.00

As shown in Table 12, after eight weeks, the nutritional cream of Formulation Example 4, which contains both Sanctuide A and Gluconic Acid, is a conventional exfoliant in Comparative Example 1, Sancunoride A containing only Sanctuide A. The ΔL value was much higher than that of Comparative Example 2 containing lactic acid and a control containing nothing of these components. In other words, the composition of the present invention containing Sancu Noride A and gluconic acid shows an excellent whitening effect compared to the conventional whitening cosmetic composition, it can be seen that the whitening effect is synergistically increased by the mixing of the two components.

Experimental Example 5: Stability Test of Formulation

The cosmetic formulations of Formulation Example 4, Comparative Example 1 and Comparative Example 2 were stored in an opaque container at constant temperature maintained at 45 ° C. or 4 ° C. for 12 weeks, and then the degree of separation and discoloration were measured. At this time, the degree of product separation and discoloration were evaluated by classifying into the following six grades. The results are shown in Table 13.

<Decision Criteria>

0: No change 1: Very slight discoloration (separation)

2: slightly discolored (separation) 3: slightly discolored (separation)

4: severely discolored (separated) 5: extremely severely discolored (separated)

Temperature The degree of discoloration of the product according to prescription Formulation Example 4 Comparative Example 1 Comparative Example 2 45 ℃ 0 0 0 4 ℃ 0 0 0

As can be seen in Table 13, there was almost no discoloration in Formulation Example 4, Comparative Example 1 and Comparative Example 2 it was found that the cosmetic of the present invention is excellent in the stability of the formulation.

Experimental Example 6: Skin irritation test of gluconic acid

Formulation Example 4, Comparative Example 1 and Comparative Example 2 were used to measure the skin irritation of gluconic acid. To test the skin irritation of the prepared formulations, a patch test was carried out using a Haye's Test Chamber in 20 healthy men and women. The test site was wiped with 70% ethanol and dried, followed by dropping 15 μg or 15 μl of the prepared test substance (Formulation Example 4, Comparative Example 1 and Comparative Example 2) (finn chamber, 100 × 10, EPITEST, Finland). Was sealed in the forearm inner part of the test subject. The patch was applied for 24 hours, the patch was removed, and the test site was marked with a marker pen. 1 hour and 24 hours after each removal (1 hour after removing the patch, 24 hours = 24 hours, 48 hours including patch attachment) using a magnifying glass (8MC-150, DAZOR, United States) to observe the test site for erythema and edema Was observed. Skin response was determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) (Table 14), the average skin reactivity was calculated by the following equation 1 and the results are shown in Table 15.

Symbol score Evaluation standard - 0  No response, normal skin ± 0.5  Faint or light erythema + 1.0  Boundary but weak erythema, edema and papules ++ 2.0  Pronounced erythema, papules and vesicles +++ 3.0  Severe erythema and vesicle formation ++++ 6.0  Cannon formation

Test substance 24 hours 48 hours Average skin reactivity (n = 20) ± + ++ ± + ++ Formulation Example 4 3 2 0 One 0 0 1.67 Comparative Example 1 2 2 0 One 0 0 1.46 Comparative Example 2 4 3 0 3 2 0 3.54

As shown in Table 15, the irritation degree of the formulation example 4 containing gluconic acid was significantly lower than that in the comparative example 2 containing lactic acid, which is a conventional keratin remover, showed a high skin irritation. In addition, it was found that the degree of irritation was not high when compared with Comparative Example 1 containing no gluconic acid or lactic acid. Therefore, it can be seen that gluconic acid, which is an active ingredient of the cosmetic composition of the present invention, is a safe ingredient that hardly causes skin irritation.

As described in detail above, the present invention provides a cosmetic composition for skin whitening, which comprises Sancunolide A and gluconic acid as active ingredients. The cosmetic composition of the present invention provides an improved whitening effect by complementarily acting on skin whitening by the complex action of the raw quinolide A having melanin production inhibitory effect and gluconic acid having melanin removal effect accumulated in keratin, The active ingredients, Sancunolide A and Gluconic Acid, are natural extracts and food additives, respectively, and do not irritate the skin and have excellent safety of the formulation, and thus can be usefully used as a cosmetic composition for skin whitening.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, which is not limited to the scope of the present invention. Therefore, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (6)

Cosmetic composition for skin whitening, including Sankunolide A (Senkunolide A) and gluconic acid (Gluconic acid) as an active ingredient. The cosmetic composition for skin whitening according to claim 1, wherein the Sancunolide A is separated from the uterus. [Claim 2] The cosmetic composition for skin whitening according to claim 1, wherein the Sancunolide A is chemically synthesized. The cosmetic composition for skin whitening according to claim 1, wherein the content of the quinomolide A is 0.00001 to 20.0 wt% based on the total weight of the cosmetic composition. The cosmetic composition for skin whitening according to claim 1, wherein the content of gluconic acid is 0.001 to 10.0 wt% based on the total weight of the cosmetic composition. The group of claim 1 wherein the composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition for skin whitening, characterized in that the formulation is selected from.