CN115957164B - Composite extract, application thereof and cosmetics - Google Patents
Composite extract, application thereof and cosmetics Download PDFInfo
- Publication number
- CN115957164B CN115957164B CN202211647277.4A CN202211647277A CN115957164B CN 115957164 B CN115957164 B CN 115957164B CN 202211647277 A CN202211647277 A CN 202211647277A CN 115957164 B CN115957164 B CN 115957164B
- Authority
- CN
- China
- Prior art keywords
- extract
- gtcc
- sample
- ginseng
- ganoderma lucidum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000284 extract Substances 0.000 title claims abstract description 142
- 239000002537 cosmetic Substances 0.000 title claims abstract description 22
- 239000002131 composite material Substances 0.000 title description 6
- 238000000605 extraction Methods 0.000 claims abstract description 40
- 150000001875 compounds Chemical class 0.000 claims abstract description 36
- 241000208340 Araliaceae Species 0.000 claims abstract description 29
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 28
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 28
- 235000008434 ginseng Nutrition 0.000 claims abstract description 28
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 27
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 24
- 241000222336 Ganoderma Species 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 95
- 240000008397 Ganoderma lucidum Species 0.000 claims description 47
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims description 47
- 235000007189 Oryza longistaminata Nutrition 0.000 claims description 33
- 240000007594 Oryza sativa Species 0.000 claims description 33
- 235000007164 Oryza sativa Nutrition 0.000 claims description 33
- 239000007788 liquid Substances 0.000 claims description 22
- 238000002360 preparation method Methods 0.000 claims description 22
- 239000002904 solvent Substances 0.000 claims description 20
- 235000020710 ginseng extract Nutrition 0.000 claims description 16
- 238000001914 filtration Methods 0.000 claims description 13
- 239000004519 grease Substances 0.000 claims description 11
- 235000002789 Panax ginseng Nutrition 0.000 claims description 7
- 238000002390 rotary evaporation Methods 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 6
- 238000002604 ultrasonography Methods 0.000 claims description 5
- 230000004580 weight loss Effects 0.000 claims description 4
- 238000010025 steaming Methods 0.000 claims description 3
- 239000002671 adjuvant Substances 0.000 claims 1
- 230000002195 synergetic effect Effects 0.000 abstract description 14
- 241000196324 Embryophyta Species 0.000 abstract description 11
- 230000003712 anti-aging effect Effects 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 83
- 239000003921 oil Substances 0.000 description 61
- 235000019198 oils Nutrition 0.000 description 61
- UILPJVPSNHJFIK-UHFFFAOYSA-N Paeonol Chemical group COC1=CC=C(C(C)=O)C(O)=C1 UILPJVPSNHJFIK-UHFFFAOYSA-N 0.000 description 54
- 230000000694 effects Effects 0.000 description 27
- YLTGFGDODHXMFB-UHFFFAOYSA-N isoacetovanillon Natural products COC1=CC=C(C(C)=O)C=C1O YLTGFGDODHXMFB-UHFFFAOYSA-N 0.000 description 27
- MLIBGOFSXXWRIY-UHFFFAOYSA-N paeonol Natural products COC1=CC=C(O)C(C(C)=O)=C1 MLIBGOFSXXWRIY-UHFFFAOYSA-N 0.000 description 27
- 235000019441 ethanol Nutrition 0.000 description 24
- 235000006708 antioxidants Nutrition 0.000 description 22
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 18
- 102000016387 Pancreatic elastase Human genes 0.000 description 18
- 108010067372 Pancreatic elastase Proteins 0.000 description 18
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical group [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 18
- 238000002835 absorbance Methods 0.000 description 17
- 239000000243 solution Substances 0.000 description 17
- 230000007760 free radical scavenging Effects 0.000 description 16
- 238000000034 method Methods 0.000 description 15
- 240000005001 Paeonia suffruticosa Species 0.000 description 13
- 235000003889 Paeonia suffruticosa Nutrition 0.000 description 13
- 239000004480 active ingredient Substances 0.000 description 13
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 12
- 230000002292 Radical scavenging effect Effects 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 11
- 229940089161 ginsenoside Drugs 0.000 description 11
- 229930182494 ginsenoside Natural products 0.000 description 11
- 230000005764 inhibitory process Effects 0.000 description 11
- -1 oryzanol Substances 0.000 description 11
- 239000012488 sample solution Substances 0.000 description 10
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 9
- 230000002849 elastaseinhibitory effect Effects 0.000 description 9
- 239000000843 powder Substances 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 239000002994 raw material Substances 0.000 description 9
- 150000003648 triterpenes Chemical class 0.000 description 9
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 8
- 241000736199 Paeonia Species 0.000 description 8
- 235000006484 Paeonia officinalis Nutrition 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 238000001514 detection method Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 239000012086 standard solution Substances 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- 238000005303 weighing Methods 0.000 description 7
- MIJYXULNPSFWEK-GTOFXWBISA-N 3beta-hydroxyolean-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C MIJYXULNPSFWEK-GTOFXWBISA-N 0.000 description 6
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- JKLISIRFYWXLQG-UHFFFAOYSA-N Epioleonolsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4CCC3C21C JKLISIRFYWXLQG-UHFFFAOYSA-N 0.000 description 6
- YBRJHZPWOMJYKQ-UHFFFAOYSA-N Oleanolic acid Natural products CC1(C)CC2C3=CCC4C5(C)CCC(O)C(C)(C)C5CCC4(C)C3(C)CCC2(C1)C(=O)O YBRJHZPWOMJYKQ-UHFFFAOYSA-N 0.000 description 6
- MIJYXULNPSFWEK-UHFFFAOYSA-N Oleanolinsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C MIJYXULNPSFWEK-UHFFFAOYSA-N 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 229940100243 oleanolic acid Drugs 0.000 description 6
- HZLWUYJLOIAQFC-UHFFFAOYSA-N prosapogenin PS-A Natural products C12CC(C)(C)CCC2(C(O)=O)CCC(C2(CCC3C4(C)C)C)(C)C1=CCC2C3(C)CCC4OC1OCC(O)C(O)C1O HZLWUYJLOIAQFC-UHFFFAOYSA-N 0.000 description 6
- 239000011550 stock solution Substances 0.000 description 6
- 238000013329 compounding Methods 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 229960000583 acetic acid Drugs 0.000 description 4
- 230000003110 anti-inflammatory effect Effects 0.000 description 4
- 230000003064 anti-oxidating effect Effects 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 239000012362 glacial acetic acid Substances 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 4
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 4
- 235000012141 vanillin Nutrition 0.000 description 4
- OCZVHBZNPVABKX-UHFFFAOYSA-N 1,1-diphenyl-2-(2,4,6-trinitrophenyl)hydrazine;ethanol Chemical compound CCO.[O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1NN(C=1C=CC=CC=1)C1=CC=CC=C1 OCZVHBZNPVABKX-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- ZTOJFFHGPLIVKC-CLFAGFIQSA-N abts Chemical compound S/1C2=CC(S(O)(=O)=O)=CC=C2N(CC)C\1=N\N=C1/SC2=CC(S(O)(=O)=O)=CC=C2N1CC ZTOJFFHGPLIVKC-CLFAGFIQSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 235000008390 olive oil Nutrition 0.000 description 3
- 239000004006 olive oil Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 235000015112 vegetable and seed oil Nutrition 0.000 description 3
- 239000008158 vegetable oil Substances 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 239000005632 Capric acid (CAS 334-48-5) Substances 0.000 description 2
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000004909 Moisturizer Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 230000003042 antagnostic effect Effects 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000011208 chromatographic data Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000032050 esterification Effects 0.000 description 2
- 238000005886 esterification reaction Methods 0.000 description 2
- 239000003574 free electron Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 238000010832 independent-sample T-test Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 230000001333 moisturizer Effects 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 229960002446 octanoic acid Drugs 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000002516 radical scavenger Substances 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- QEVHRUUCFGRFIF-MDEJGZGSSA-N reserpine Chemical compound O([C@H]1[C@@H]([C@H]([C@H]2C[C@@H]3C4=C(C5=CC=C(OC)C=C5N4)CCN3C[C@H]2C1)C(=O)OC)OC)C(=O)C1=CC(OC)=C(OC)C(OC)=C1 QEVHRUUCFGRFIF-MDEJGZGSSA-N 0.000 description 2
- 230000009759 skin aging Effects 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 239000000516 sunscreening agent Substances 0.000 description 2
- 238000009210 therapy by ultrasound Methods 0.000 description 2
- 238000002137 ultrasound extraction Methods 0.000 description 2
- WMBWREPUVVBILR-WIYYLYMNSA-N (-)-Epigallocatechin-3-o-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-WIYYLYMNSA-N 0.000 description 1
- KZJWDPNRJALLNS-VPUBHVLGSA-N (-)-beta-Sitosterol Natural products O[C@@H]1CC=2[C@@](C)([C@@H]3[C@H]([C@H]4[C@@](C)([C@H]([C@H](CC[C@@H](C(C)C)CC)C)CC4)CC3)CC=2)CC1 KZJWDPNRJALLNS-VPUBHVLGSA-N 0.000 description 1
- CSVWWLUMXNHWSU-UHFFFAOYSA-N (22E)-(24xi)-24-ethyl-5alpha-cholest-22-en-3beta-ol Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(CC)C(C)C)C1(C)CC2 CSVWWLUMXNHWSU-UHFFFAOYSA-N 0.000 description 1
- KLEXDBGYSOIREE-UHFFFAOYSA-N 24xi-n-propylcholesterol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)CCC(CCC)C(C)C)C1(C)CC2 KLEXDBGYSOIREE-UHFFFAOYSA-N 0.000 description 1
- LPZCCMIISIBREI-MTFRKTCUSA-N Citrostadienol Natural products CC=C(CC[C@@H](C)[C@H]1CC[C@H]2C3=CC[C@H]4[C@H](C)[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C)C(C)C LPZCCMIISIBREI-MTFRKTCUSA-N 0.000 description 1
- ARVGMISWLZPBCH-UHFFFAOYSA-N Dehydro-beta-sitosterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)CCC(CC)C(C)C)CCC33)C)C3=CC=C21 ARVGMISWLZPBCH-UHFFFAOYSA-N 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 102000016942 Elastin Human genes 0.000 description 1
- 108010014258 Elastin Proteins 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WMBWREPUVVBILR-UHFFFAOYSA-N GCG Natural products C=1C(O)=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-UHFFFAOYSA-N 0.000 description 1
- UFNDONGOJKNAES-UHFFFAOYSA-N Ginsenoside Rb1 Natural products CC(=CCCC(C)(OC1OC(COC2OC(CO)C(O)C(O)C2O)C(O)C(O)C1O)C3CCC4(C)C3C(O)CC5C6(C)CCC(OC7OC(CO)C(O)C(O)C7OC8OC(CO)C(O)C(O)C8O)C(C)(C)C6CC(O)C45C)C UFNDONGOJKNAES-UHFFFAOYSA-N 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 102000002274 Matrix Metalloproteinases Human genes 0.000 description 1
- 108010000684 Matrix Metalloproteinases Proteins 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 235000002791 Panax Nutrition 0.000 description 1
- 241000208343 Panax Species 0.000 description 1
- 241000222341 Polyporaceae Species 0.000 description 1
- 241000218201 Ranunculaceae Species 0.000 description 1
- 235000003434 Sesamum indicum Nutrition 0.000 description 1
- 244000000231 Sesamum indicum Species 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- YURJSTAIMNSZAE-UHFFFAOYSA-N UNPD89172 Natural products C1CC(C2(CC(C3C(C)(C)C(O)CCC3(C)C2CC2O)OC3C(C(O)C(O)C(CO)O3)O)C)(C)C2C1C(C)(CCC=C(C)C)OC1OC(CO)C(O)C(O)C1O YURJSTAIMNSZAE-UHFFFAOYSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- MJVXAPPOFPTTCA-UHFFFAOYSA-N beta-Sistosterol Natural products CCC(CCC(C)C1CCC2C3CC=C4C(C)C(O)CCC4(C)C3CCC12C)C(C)C MJVXAPPOFPTTCA-UHFFFAOYSA-N 0.000 description 1
- LGJMUZUPVCAVPU-UHFFFAOYSA-N beta-Sitostanol Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(C)CCC(CC)C(C)C)C1(C)CC2 LGJMUZUPVCAVPU-UHFFFAOYSA-N 0.000 description 1
- NJKOMDUNNDKEAI-UHFFFAOYSA-N beta-sitosterol Natural products CCC(CCC(C)C1CCC2(C)C3CC=C4CC(O)CCC4C3CCC12C)C(C)C NJKOMDUNNDKEAI-UHFFFAOYSA-N 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 208000007287 cheilitis Diseases 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229920002549 elastin Polymers 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- GCFHZZWXZLABBL-UHFFFAOYSA-N ethanol;hexane Chemical compound CCO.CCCCCC GCFHZZWXZLABBL-UHFFFAOYSA-N 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- GZYPWOGIYAIIPV-JBDTYSNRSA-N ginsenoside Rb1 Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)O[C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(CC[C@H]4C(C)(C)[C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC[C@]4(C)[C@H]3C[C@H]2O)C)(C)CC1)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GZYPWOGIYAIIPV-JBDTYSNRSA-N 0.000 description 1
- TXEWRVNOAJOINC-UHFFFAOYSA-N ginsenoside Rb2 Natural products CC(=CCCC(OC1OC(COC2OCC(O)C(O)C2O)C(O)C(O)C1O)C3CCC4(C)C3C(O)CC5C6(C)CCC(OC7OC(CO)C(O)C(O)C7OC8OC(CO)C(O)C(O)C8O)C(C)(C)C6CCC45C)C TXEWRVNOAJOINC-UHFFFAOYSA-N 0.000 description 1
- PWAOOJDMFUQOKB-WCZZMFLVSA-N ginsenoside Re Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](O[C@@H]2[C@H]3C(C)(C)[C@@H](O)CC[C@]3(C)[C@@H]3[C@@]([C@@]4(CC[C@@H]([C@H]4[C@H](O)C3)[C@](C)(CCC=C(C)C)O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)C)(C)C2)O[C@H](CO)[C@@H](O)[C@@H]1O PWAOOJDMFUQOKB-WCZZMFLVSA-N 0.000 description 1
- YURJSTAIMNSZAE-HHNZYBFYSA-N ginsenoside Rg1 Chemical compound O([C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(C[C@@H]([C@H]4C(C)(C)[C@@H](O)CC[C@]4(C)[C@H]3C[C@H]2O)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)C)(C)CC1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O YURJSTAIMNSZAE-HHNZYBFYSA-N 0.000 description 1
- CBEHEBUBNAGGKC-UHFFFAOYSA-N ginsenoside Rg1 Natural products CC(=CCCC(C)(OC1OC(CO)C(O)C(O)C1O)C2CCC3(C)C2C(O)CC4C5(C)CCC(O)C(C)(C)C5CC(OC6OC(CO)C(O)C(O)C6O)C34C)C CBEHEBUBNAGGKC-UHFFFAOYSA-N 0.000 description 1
- AOGZLQUEBLOQCI-UHFFFAOYSA-N ginsenoside-Re Natural products CC1OC(OCC2OC(OC3CC4(C)C(CC(O)C5C(CCC45C)C(C)(CCC=C(C)C)OC6OC(CO)C(O)C(O)C6O)C7(C)CCC(O)C(C)(C)C37)C(O)C(O)C2O)C(O)C(O)C1O AOGZLQUEBLOQCI-UHFFFAOYSA-N 0.000 description 1
- 239000008269 hand cream Substances 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000010829 isocratic elution Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 235000015500 sitosterol Nutrition 0.000 description 1
- KZJWDPNRJALLNS-VJSFXXLFSA-N sitosterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CC[C@@H](CC)C(C)C)[C@@]1(C)CC2 KZJWDPNRJALLNS-VJSFXXLFSA-N 0.000 description 1
- NLQLSVXGSXCXFE-UHFFFAOYSA-N sitosterol Natural products CC=C(/CCC(C)C1CC2C3=CCC4C(C)C(O)CCC4(C)C3CCC2(C)C1)C(C)C NLQLSVXGSXCXFE-UHFFFAOYSA-N 0.000 description 1
- 229950005143 sitosterol Drugs 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
- 230000000475 sunscreen effect Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 238000013417 toxicology model Methods 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Abstract
The invention relates to the technical field of plant extraction, and particularly provides a compound extract, application thereof and cosmetics. Compared with the prior art, the compound extract obtained according to the specific proportion has antioxidant and anti-aging activities; compared with single cortex moutan extract, ginseng radix extract or Ganoderma extract, has remarkable synergistic effect in antioxidant activity.
Description
Technical Field
The invention relates to the technical field of plant extraction, in particular to a compound extract, application thereof and cosmetics.
Background
In recent years, cosmetics with plant concepts are popular, and development of the cosmetics have become mainstream trends in the cosmetics industry. In the compendium of materia medica, 168 Chinese herbal medicines for beautifying in the traditional Chinese herbal medicine are summarized, and at present, plant raw materials are mostly applied in cosmetics in the form of extracts. For color cosmetics, the product with the functions of nourishing and protecting skin can make the product have a qualitative leap.
Cortex moutan is dried root bark of Paeonia suffruticosa of Ranunculaceae. Produced in Anhui, sichuan, henan and Shandong provinces. Bitter and pungent, slightly cold, and the effects of clearing heat and cooling blood, activating blood and dissolving stasis, removing deficiency heat and the like, and can restore heart, liver and kidney meridians.
Ganoderma lucidum is called a precious traditional Chinese medicine for strengthening body resistance and strengthening body, belongs to medicinal fungi of Polyporaceae, and has been used for treating diseases such as liver diseases, high cholesterol, hyperlipidemia and the like. The main antioxidant active ingredients in ganoderma lucidum are known as ganoderma lucidum triterpene and ganoderma lucidum polysaccharide. The ganoderma lucidum is divided into three growth stages: spores, hyphae, fruiting bodies, ganoderma lucidum as the second growth stage.
Ginseng is perennial herb of Panax of Araliaceae. Stems, leaves, flowers, fruits and processing byproducts of ginseng are raw materials of the light industry, and can be processed into commodities such as cigarettes, wines, teas, crystals, pastes and the like containing ginseng components.
The prior art has not been studied about compounding of cortex moutan extract, ganoderma lucidum extract and ginseng extract and applying the compounded product to an antioxidant product.
Disclosure of Invention
In view of the above, the present invention aims to provide a compound extract, its application and cosmetics, wherein the compound extract has antioxidant and antiaging activities; compared with single cortex moutan extract, ginseng radix extract or Ganoderma extract, the composition has remarkable synergistic effect in antioxidant effect.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
A compound extract comprises cortex moutan extract, ginseng radix extract and Ganoderma extract.
The main component of the tree peony bark extract is paeonol, which can obviously absorb UVB ultraviolet rays, has wide wavelength coverage, and can be used as a sun-screening agent of cosmetics; the wide pharmacological action of paeonol is mainly based on the comprehensive action of antioxidant and anti-inflammatory mechanisms, and the clinical application of paeonol is mainly concentrated in two parts, wherein one part is to use paeonol as an ointment for treating skin inflammation, and the anti-inflammatory property of paeonol has prevention and treatment effects on various skin diseases such as wet diseases and the like. Considering that consumers can play a role in improving aging requirements caused by lip inflammation, paeonol is taken as a main active substance to be examined in cortex moutan raw materials in subsequent experiments.
The compound extract of the invention comprises the following components in parts by weight:
10-75 parts of tree peony bark extract;
5-60 parts by weight of ginseng extract;
4-80 parts of ganoderma lucidum extract.
The compound extract of the invention preferably comprises the following components in parts by weight:
35-72 parts of tree peony bark extract;
12-46 parts by weight of ginseng extract;
4-49 parts of ganoderma lucidum extract.
In one embodiment of the invention, the compound extract comprises the following components in parts by weight:
71 parts by weight of moutan bark extract;
12 parts by weight of ginseng extract;
17 parts of ganoderma lucidum extract.
In one embodiment of the invention, the compound extract comprises the following components in parts by weight:
50 parts of tree peony bark extract;
46 parts by weight of ginseng extract;
4 parts of ganoderma lucidum extract.
In the present invention, the preparation method of the tree peony bark extract comprises: extracting cortex moutan with extraction solvent at 20-30deg.C for 50-80 min at a feed-liquid ratio of 1 (10-15);
The extraction solvent consists of 25wt% of red rice oil, 25wt% of ethanol and 50wt% of GTCC;
the frequency of the ultrasonic wave is 20-30 kHz.
The preparation method of the tree peony bark extract preferably comprises the following steps: extracting cortex moutan with extraction solvent at room temperature at a feed-liquid ratio of 1:15 for 60min;
The frequency of the ultrasound was 25.6kHz.
The preparation method of the tree peony bark extract preferably comprises the following steps: taking cortex moutan powder, adding a mixed extractant (25% red rice oil, 25% ethanol and 50% GTCC) into the powder according to the ratio of feed liquid to material of 1:15 at normal temperature, simultaneously carrying out ultrasound at 25.6kHz, keeping the initial temperature consistent, stirring at 60r/min, carrying out ultrasound extraction for 60min, filtering with filter paper to remove residues, removing ethanol by rotary evaporation, and supplementing the weight loss of each step with mixed grease (red rice oil: GTCC=1:2) to obtain the cortex moutan extract.
The GTCC in the invention is high-purity grease formed by esterification of caprylic acid/capric acid and glycerin, has good spreadability, and ensures that skin has a slippery but not greasy feeling; is easy to be absorbed by skin; the cosmetic has good effect on the uniformity and the fineness of cosmetics, so that the skin is lubricated and glossy; meanwhile, the composition can be used as a base material of a moisturizing factor, a stabilizer of cosmetics, an antifreezing agent and a homogenizing agent. Red rice belongs to a medicinal and edible resource, is rich in proteins, amino acids, vitamins, microelements and functional active ingredients, and is reported to have good anti-inflammatory and antioxidant effects. Oryzanol in red rice has good efficacy of stabilizing fat and grease, and can accelerate blood circulation and metabolism of skin; absorbing ultraviolet rays, preventing erythema caused by the ultraviolet rays, and inhibiting tyrosinase activity; can be used as moisturizer, skin conditioner, whitening and freckle removing agent and antioxidant in cosmetics, and has effects of nourishing circulation, nourishing skin and preventing skin aging. The red rice oil is used as natural vegetable oil, has stable oil quality and low viscosity, and is not easy to cause greasy feeling when being smeared; has good affinity and permeability with skin; wherein the product is rich in natural active ingredients such as oryzanol, vitamin E, sitosterol, etc. In addition, the red rice oil can extract fat-soluble active ingredients with different polarities, such as paeonol, ganoderma triterpene, ginsenoside and the like, from various plant raw materials, so that the oil extract has remarkable efficacy.
In the present invention, the ginseng extract is red ginseng rootlet extract. Ginseng radix Rubri has antioxidant, antiaging and antifatigue effects. Ginsenoside is main active ingredient of Ginseng radix Rubri, is amphoteric substance, and has biological activity of resisting oxidation and aging.
In the present invention, the method for preparing ginseng extract comprises: extracting ginseng by using an extraction solvent at the temperature of 20-30 ℃ and the feed-liquid ratio of 1 (10-15), wherein the extraction time is 50-80 min;
The extraction solvent consists of 25wt% of red rice oil, 25wt% of ethanol and 50wt% of GTCC;
the frequency of the ultrasonic wave is 20-30 kHz.
The preparation method of the ginseng extract preferably comprises the following steps: extracting Ginseng radix with extraction solvent at room temperature at a feed-liquid ratio of 1:15 for 60min;
The frequency of the ultrasound was 25.6kHz.
The preparation method of the ginseng extract of the present invention preferably comprises: mixing Ginseng radix powder at a ratio of 1:15, adding mixed extractant (25% red rice oil, 25% ethanol and 50% GTCC) at room temperature, simultaneously performing ultrasonic treatment at 25.6kHz, stirring at 60r/min, ultrasonic extracting for 60min, filtering with filter paper to remove residue, steaming to remove ethanol, and adding mixed oil (red rice oil: GTCC=1:2) to obtain Ginseng radix extract.
In the invention, the ganoderma lucidum extract is a ganoderma lucidum fruiting body extract. The modern medical research reveals that the ganoderma lucidum has pharmacological effects of resisting tumor, enhancing immunity, reducing blood sugar and the like, and mainly contains active ingredients such as triterpene, polysaccharide, nucleoside and alkaloid and the like. The ganoderma triterpene has high fat solubility, has the activities of antioxidation, antibiosis, antivirus and the like, and is one of important indexes for identifying the quality of ganoderma.
In the invention, the preparation method of the ganoderma lucidum extract comprises the following steps: extracting ganoderma lucidum with an extraction solvent at 20-30 ℃ for 50-80 min, wherein the feed-liquid ratio is1 (10-15);
The extraction solvent consists of 25wt% of red rice oil, 25wt% of ethanol and 50wt% of GTCC;
the frequency of the ultrasonic wave is 20-30 kHz.
The preparation method of the ganoderma lucidum extract preferably comprises the following steps: extracting Ganoderma with extraction solvent at room temperature at a feed-liquid ratio of 1:15 for 60min;
The frequency of the ultrasound was 25.6kHz.
The preparation method of the ganoderma lucidum extract preferably comprises the following steps: mixing Ganoderma powder at a ratio of 1:15, adding mixed extractant (25% red rice oil, 25% ethanol and 50% GTCC) into the powder at normal temperature, simultaneously performing ultrasonic treatment at 25.6kHz, stirring at 60r/min, ultrasonic extracting for 60min, filtering with filter paper to remove residue, steaming to remove ethanol, and adding mixed oil (red rice oil: GTCC=1:2) to obtain Ganoderma extract.
The invention also provides application of the compound extract in preparing antioxidant products.
In the present invention, the antioxidant is DPPH free radical scavenging.
The invention also provides cosmetics, which comprise the compound extract and cosmetically acceptable auxiliary materials.
The cosmetic of the present invention is preferably a lotion, an emulsion, a foundation, an essence, a mask, a sunscreen, a face cream, an eye cream, a hand cream or a body cream; the mask is preferably a patch type mask or a smearing type mask; the essence is preferably essence water or essence oil.
The auxiliary materials are acceptable auxiliary materials in cosmetics, and are preferably one or more of solvents, thickeners, moisturizers, film forming agents, liposomes, preservatives, colorants, pH regulators and essence; the solvent is preferably water or an alcohol solvent.
The invention adopts grease to extract active ingredients with fat solubility in natural products (red sesame, red ginseng, tree peony bark and red rice oil) with homology of medicine and food, and compounds the extracts and evaluates the effects of antioxidation and anti-aging, calculates the degree of synergy of compound samples by an isoradiometric analysis method, and inspects the synergy between single samples, thereby providing a method and a basis for evaluating the activity of the oil extract of the compound natural products and the feasibility of the oil extract in color cosmetic application, and the invention has the following beneficial effects:
(1) The traditional oil extraction method of medicinal plants is mainly to fry and extract raw materials at high temperature by using vegetable oil, and the high temperature condition of the traditional process is not only easy to destroy active ingredients in plants, but also easy to cause rancidity of the vegetable oil. Compared with the traditional frying technology, the invention has the advantages of less loss of fat-soluble active ingredients in the compound extract sample, good sample stability, simple operation technology and low equipment requirement, and the oil extract can be directly used in the color cosmetic product after the ethanol is removed by rotary evaporation. In addition, oryzanol rich in red rice oil improves the antioxidation capability and the moistening degree of the sample; GTCC is high-purity grease formed by esterification of caprylic acid/capric acid and glycerin, has good spreadability, and can enhance the stability and solubility of a sample.
(2) The invention utilizes biochemical method to evaluate antioxidant and anti-aging activities of the composite extracts of ganoderma lucidum, ginseng and tree peony bark respectively from three indexes of DPPH free radical scavenging ability, ABTS free radical reducing ability and elastase inhibiting ability, and simultaneously calculates the degree of synergy of the composite samples by an isoradiometric analysis method to investigate the synergy among single oil extracts.
Experiments prove that the highest degree of synergy of the combination K2# in the compound combination is 2.535 in terms of DPPH free radical scavenging capability, and the corresponding combination proportion is 50% of peony oil extract, 45.8% of ginseng oil extract and 4.17% of ganoderma lucidum oil extract; the synergy of the combination K1# is 2.236, and the corresponding combination proportion is 71% of the moutan bark oil extract, 12% of the ginseng oil extract and 17% of the ganoderma lucidum oil extract, and the synergy of the K1# and the K2# is not statistically different.
Drawings
FIG. 1 is a graph showing a comparison of pre-experimental data of paeonol extraction rates using different extractants;
FIG. 2 is a graph showing comparison of paeonol extraction rate experimental data under different extraction conditions in an orthogonal experiment;
FIG. 3 is a graph comparing the activities of extracts of single plants;
FIG. 4 is a graph comparing the DPPH radical scavenging ability IC50 values of single plant extracts and formulated samples;
FIG. 5 is a graph comparing VC equivalent values of extracts of single plants and compounded samples;
Fig. 6 is an equivalent front view of the DPPH radical scavenging ability of the compounded sample;
FIG. 7 is a left side view of the equivalent surface of the DPPH radical scavenging ability of the formulated sample;
FIG. 8 is a top view of the equivalent surface of the DPPH radical scavenging ability of the compounded sample;
FIG. 9 is a graph showing the DPPH radical scavenging ability and elastase inhibitory ability of the formulated samples.
FIG. 10 is a graph comparing elastase inhibition ability of a formulated sample to that of a single sample.
Detailed Description
The technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In order to further illustrate the present invention, the following examples are provided. The raw materials used in the following examples of the present invention are all commercially available. The raw materials adopted in the embodiment of the invention are commercial products, wherein GTCC is purchased from IOI ESTERCHEM (M) SDN.BHD.
1. Preparation method of cortex moutan, ginseng radix Rubri, ganoderma lucidum fruiting body extract and analysis of main active components
1.1 Optimization of extraction Process
Because of the excellent lipid solubility, anti-inflammatory property and relatively low cost of the paeonol raw material, the extraction agent and the extraction process are optimized by taking paeonol as a target object of the active ingredient.
Comparative test
Respectively extracting with olive oil, red rice oil, GTCC and 2EHP by ultrasonic extraction method; and respectively carrying out auxiliary extraction on paeonol by respectively fusing olive oil, red rice oil, GTCC (gtCC), and 2EHP with absolute ethyl alcohol (volume ratio is 1:1), and taking paeonol extraction rate and physical state of a sample as evaluation indexes (without optimizing an extraction process, namely, without considering ultrasonic time, ultrasonic frequency and the like). The results are shown in FIG. 1.
As can be seen from fig. 1, the extraction yield under the ethanol-assisted extraction is higher than that of the single extraction solvent, wherein GTCC-ethanol and red rice oil-ethanol are higher. And the extract obtained by extracting the tree peony bark after the olive oil and the ethanol are compounded is sticky and has a peculiar smell, and the 2EHP is limited in use in the cosmetic industry. The red rice oil is rich in oryzanol, has better antioxidant capacity, stable GTCC and strong fluidity, so that the red rice oil and the GTCC are finally selected as main components of the extractant, and the absolute ethyl alcohol is used for auxiliary extraction, so that the consumption of the absolute ethyl alcohol is reduced as much as possible under the condition of improving the extraction rate of paeonol as much as possible.
1.1.1 Preparation of extracts
The cortex moutan powder was placed in a 250mL beaker and subjected to orthogonal experiments (total amount of extractant kept consistent) as shown in Table 1 and Table 2.3 times of each experiment are repeated, the initial temperature is kept consistent, stirring is carried out at 60r/min, filter paper is subjected to suction filtration (such as centrifugation at 4000rpm-5000rpm for turbidity), dregs are removed, ethanol is removed by rotary evaporation (the rotary evaporation temperature is 40 ℃, the rotating speed is constant, the liquid level in a water bath kettle exceeds the liquid level of a flask, the rotary evaporation time of different ethanol proportions is recorded, the time of different parallels in the same level is kept consistent), and the filtrate is weighed to compensate the loss weight of corresponding oil.
Taking about 0.25g of oil sample into a 10mL centrifuge tube, dissolving with 5mL of methanol, centrifuging for 10min at 8000r/min, taking supernatant, storing in a liquid storage bottle, and measuring.
The low frequency is 25.6kHz, the intermediate frequency is 45.8kHz, and the high frequency is 69.8kHz.
TABLE 1 factor level
TABLE 2 orthogonal experimental design table
1.1.2 Detection of paeonol content of extract
The liquid chromatography conditions were as follows. Chromatographic column: epider C18 Hypersil ODS 5 μm (. Phi.4.60 mm. Times.250 mm); mobile phase: a: b=0.1% phosphoric acid: acetonitrile, isocratic elution a: b=25:75; analysis time: 5min; the flow rate is 1.0mL/min; column temperature is 30 ℃; the sample injection amount is 10 mu L; detection wavelength: 274nm.
Standard stock solution of paeonol 0.2 mg/mL: precisely weighing 1mg paeonol standard substance, placing into 5mL brown volumetric flask, dissolving in methanol, fixing volume to scale, shaking, and preserving at 4deg.C.
The method comprises the following steps: standard solutions of paeonol were prepared at concentrations of 10ng/mL, 50ng/mL, 500ng/mL, 1. Mu.g/mL, 10. Mu.g/mL, and 50. Mu.g/mL, respectively, using standard stock solutions of paeonol. And (3) performing HPLC analysis, respectively sampling each concentration for 6 times, taking the peak area average value, drawing a standard curve, and calculating the precision. The detection limit and the quantitative limit are calculated by chromatographic data of 10ng/mL paeonol standard solution.
1ML of the sample solution in the volumetric flask was taken, and the filtration membrane (0.45 μm organic filtration membrane) was placed in a liquid phase vial. Sampling by HPLC for 3 times, taking average value, calculating the concentration of the sample liquid by using a standard curve, and calculating the paeonol content in the sample.
1.1.3 Results
Extraction yield results of paeonol extraction in orthogonal experiments are shown in table 3 below, and influence on extraction yield: the extractant is more than ultrasonic frequency more than feed liquid ratio more than ultrasonic time. And the optimal extraction process is as follows: mixing extracting agent 25% red rice oil, 25% absolute ethanol, 50% GTCC, and extracting at low frequency and feed-liquid ratio of 1:15 for 60min. In the experiment, the extraction process also obtains the highest extraction rate. The results are shown in FIG. 2.
TABLE 3 Paeonol extraction yield results Table
1.2 Preparation of cortex moutan extract and detection of paeonol content
1.2.1 Preparation of cortex moutan extract
The cortex moutan powder is placed in a 500mL beaker, and mixed extractant (25% red rice oil, 50% GTCC,25% absolute ethanol) is added according to the ratio of 1:15, and each experiment is repeated for 3 times. Low frequency, stirring at 60r/min, ultrasonic extracting for 60min, filtering with filter paper to remove residue, rotary evaporating to remove ethanol, and weighing the filtrate to compensate for the lost weight of the corresponding oil. The obtained extract is opaque oil body, light yellow brown, and has cortex moutan smell.
About 0.25g of the oil sample is taken and placed in a10 mL centrifuge tube, 5mL of methanol is added for dissolution, centrifugation is carried out for 10min at 8000r/min, the supernatant is taken and stored in a liquid storage bottle, and the liquid is to be detected.
1.2.2 Method for detecting paeonol content
Same as 1.1.2.
1.3 Preparation of Ginseng radix Rubri extract and ginsenoside content detection
1.3.1 Preparation of Ginseng radix Rubri extract
Red ginseng rootlet powder was placed in a 500mL beaker, and mixed extractant (25% red rice oil, 50% gtcc,25% absolute ethanol) was added at 1:15, and 3 replicates were performed for each set of experiments. Low frequency, stirring at 60r/min, ultrasonic extracting for 60min, filtering with filter paper to remove residue, rotary evaporating to remove ethanol, and weighing the filtrate to compensate for the lost weight of the corresponding oil. The final extract is transparent oil body, light yellowish brown, and has characteristic smell of plant.
About 0.25g of the oil sample is taken and placed in a10 mL centrifuge tube, 5mL of methanol is added for dissolution, centrifugation is carried out for 10min at 8000r/min, the supernatant is taken and stored in a liquid storage bottle, and the liquid is to be detected.
1.3.2 Method for detecting ginsenoside content
The liquid chromatography conditions were as follows. Chromatographic column: epider C18 Hypersil ODS 5 μm (. Phi.4.60 mm. Times.250 mm); mobile phase: a: b=water: acetonitrile, gradient elution procedure is shown in table 4; analysis time: 80min; the flow rate is 1.0mL/min; column temperature is 30 ℃; the sample injection amount is 10 mu L; detection wavelength: 203nm.
Note that the oil sample should be enriched with a low level of target active.
Table 4 HPLC gradient elution procedure for detecting ginsenoside
Ginsenoside mixed stock solution: respectively precisely weighing 10mg of ginsenoside Rg1 standard substance, 10mg of ginsenoside Re standard substance and 10mg of ginsenoside Rb1 standard substance, placing all the materials into a 5mL brown volumetric flask, dissolving with methanol, and fixing volume to obtain 2mg/mL ginsenoside mixed stock solution.
The evaluation method comprises the following steps: a ginsenoside stock solution was used to prepare ginsenoside stock solutions having concentrations of 1. Mu.g/mL, 10. Mu.g/mL, 50. Mu.g/mL, 0.1mg/mL, 0.5mg/mL, and 2mg/mL, respectively. And (3) performing HPLC analysis, respectively sampling each concentration for 3 times, taking the peak area average value, drawing a standard curve, and calculating the precision. The detection limit and the quantitative limit are calculated by chromatographic data of a paeonol standard solution of 1 mug/mL.
1ML of the sample solution in the volumetric flask was taken, and the filtration membrane (0.45 μm organic filtration membrane) was placed in a liquid phase vial. Sampling by HPLC for 3 times, taking average value, calculating the concentration of the sample liquid by using a standard curve, and calculating the content of ginsenoside in the sample.
1.4 Preparation of Ganoderma lucidum fruiting body extract and Ganoderma lucidum triterpene content detection
1.4.1 Preparation of Ganoderma lucidum fruiting body extract
The fruiting body powder of Ganoderma lucidum was placed in a 500mL beaker, and mixed extractant (25% red rice oil, 50% GTCC,25% absolute ethanol) was added at a ratio of 1:15, and 3 replicates were performed for each experiment. Low frequency, stirring at 60r/min, ultrasonic extracting for 60min, filtering with filter paper to remove residue, rotary evaporating to remove ethanol, and weighing the filtrate to compensate for the lost weight of the corresponding oil. The obtained extract is transparent oil body, reddish brown, and has special sweet taste of Ganoderma.
Since other fat-soluble active ingredients in the sample have other color-developing reactions with perchloric acid-vanillin, the absorbance of the sample is greatly changed. Therefore, the simulated extractant with the normal hexane as the mixed grease extractant is used for extracting the ganoderma lucidum triterpene according to the same extraction conditions, and the normal hexane-ganoderma lucidum extract is used as a sample for detecting the triterpene substance content in the sample.
1.4.2 Method for detecting triterpene content of ganoderma lucidum
Oleanolic acid standard solution: 2mg of oleanolic acid standard is precisely weighed, placed in a 5mL brown volumetric flask, dissolved by using methanol and fixed in volume, and then 0.4mg/mL oleanolic acid standard solution is obtained.
Vanillin glacial acetic acid solution: precisely weighing 0.5g of vanillin, placing in a 10mL brown volumetric flask, adding glacial acetic acid for dissolution, fixing the volume, and oscillating to obtain vanillin glacial acetic acid solution with the concentration of 50 mg/mL.
Standard curve preparation: precisely weighing 0.2mL, 0.3mL, 0.4mL, 0.5mL, 0.6mL and 0.8mL of oleanolic acid standard solution, respectively placing into 15mL test tubes with plugs, volatilizing, cooling, precisely adding 0.2mL of newly prepared vanillin glacial acetic acid solution and 0.8mL of perchloric acid, shaking uniformly, heating in a water bath at 70 ℃ for 15 minutes, immediately cooling in the ice bath for 5 minutes, taking out, precisely adding 4mL of ethyl acetate, and shaking uniformly. The standard solution of oleanolic acid was not added as a blank in the above-described operation, and the absorbance was measured at 546nm wavelength using a spectrophotometer, and a standard curve was drawn with the absorbance as the ordinate and the concentration as the abscissa.
Precisely measuring 0.2mL of the sample solution, placing the sample solution into a 15mL test tube with a plug, operating the method in the same way from 'volatilizing', measuring absorbance, reading the content of oleanolic acid in the sample solution from a standard curve, and calculating to obtain the product.
1.5 Analysis results of the content of Main active ingredient
The paeonol content of the tree peony bark extract, the ginsenoside content of the red ginseng rootlet extract, and the ganoderma lucidum triterpene content of the ganoderma lucidum fruiting body extract are shown in Table 5.
TABLE 5 Main active ingredient content (mg/g) in extracts of cortex moutan, ginseng radix Rubri and Ganoderma lucidum fruiting body
2. Activity research of three extracts and research on compounding and synergy degree thereof
2.1 Single Activity ability assay of three extracts
2.1.1DPPH determination of free radical scavenging Capacity
DPPH (1, 1-diphenyl-2-trinitrophenylhydrazine) is a relatively stable lipid radical with a free electron on N and a purple color in ethanol solution, with a maximum absorption peak at 517 nm. After the addition of the antioxidant, DPPH captures an electron to pair with the free electron, the purple color fades to a colorless substance, the absorption at 517nm disappears, and the degree of fading is quantitatively related to the number of electrons it receives. According to the principle, a spectrophotometer is used for detecting the change of the absorbance value after DPPH free radical reacts with the sample liquid, and the capability of the sample for providing hydrogen atoms, scavenging free radicals and resisting oxidation can be detected.
Respectively diluting cortex moutan extract, ginseng radix Rubri extract, and Ganoderma lucidum fruiting body extract with n-hexane ethanol solution (n-hexane: ethanol=1:1) at different concentrations.
100 Mu L of sample solutions with different concentrations and 100 mu L of DPPH-ethanol solution with the concentration of 0.25mmol/L are respectively added into a 96-well plate by a liquid-transferring gun, uniformly mixed, and after standing for 30min, the sample is subjected to light absorption value measurement at 517 nm. Each sample was run in 3 replicates, with an average of A1, using VC as a positive control. DPPH clearance was calculated as follows:
Wherein A0 is absorbance at 517nm of 100. Mu.L of sample solvent+100. Mu.L of DPPH-ethanol; a1 is absorbance at 517nm of 100. Mu.L of sample solution +100. Mu.L of DPPH-ethanol; a2 is the absorbance at 517nm of 100. Mu.L of the sample solution+100. Mu.L of ethanol. Three in parallel per group (one 96-well plate can only make one sample).
2.1.2ABTS determination of free radical reducing Capacity
AbTS is oxidized into green AbTS + under the action of a proper oxidant, and the total antioxidant capacity of the sample can be obtained by measuring the absorbance of the AbTS at the wavelength of 734 nm.
Preparation of ABTS stock (7 mmol/L): ABTS 0.09602g was taken and distilled water 25mL was added. (mw=548.7). K 2S2O8 stock (140 mmol/L): k 2S2O8 0.94612g was taken and 25mL of distilled water was added. (M W = 270.32). Dissolving and diluting the G1#, G2#, and G3# oil extract samples by absolute ethyl alcohol. Accurately measuring 5mL of 7mmol/LABTS solution and 88 mu L of 140mmol/L (final concentration 2.45 mmol/L) potassium persulfate (K 2S2O8) solution, and uniformly mixing to prepare the ABTS working mother solution. Storing at room temperature for 12-16h in dark place. Dilution with absolute ethanol (or PBS solution at ph=7.4) prior to use requires absorbance of ABTS working fluid minus blank, a734 is 0.700±0.020. Accurately measuring 10 mu L of sample solution (positive reference substance solution) with each concentration gradient and 200 mu L of ABTS working solution in a 96-well plate, lightly mixing, standing at room temperature for reaction for 6min, and measuring the absorbance value at 734nm by using an enzyme-labeled instrument. The total antioxidant capacity was calculated as follows:
Wherein A 0 is the absorbance value of the reaction system without the sample, namely, absolute ethyl alcohol is used for replacing the sample; a 1 is the absorbance value of the sample contained in the reaction system; a 2 is the absorbance value of the reaction system containing the sample, but absolute ethyl alcohol is used instead of ABTS.
A 0: sample lysis reagent 10. Mu.L+ABTS 200. Mu.L;
A 1 sample 10. Mu.L+ABTS 200. Mu.L
A 2 sample 10. Mu.L+200. Mu.L ethanol
2.1.3 Measurement of elastase inhibitory Capacity
Elastase is one of the most important enzymes in the matrix metalloproteinase family, secreted synthetically by fibroblasts, and is capable of degrading elastin in the skin leading to skin aging.
Method for determining the inhibitory activity of Porcine Pancreatic Elastase (PPE): using AAANA as substrate, 100. Mu.L of 50mmol/L Tris-HCl buffer (pH 8.0) was mixed with 50. Mu.L of sample solutions of different mass concentrations, 25. Mu.L of 1.015mmol/L AAANA solution was added, incubation was carried out at 37℃for 15min, followed by 25. Mu.L of elastase solution (0.3U/mL) and absorbance at 410nm was measured after 15min, and elastase solution and AAANA solution were each formulated with 50mmol/L Tris-HCl buffer (pH 8.0) and EGCG was used as positive control, and assayed in parallel for 3 times. The inhibition ratio of elastase by the sample was calculated according to the following formula.
/>
Wherein: a is the absorbance of the reaction solution without the sample; b is the absorbance of the reaction solution containing the sample.
2.2, Ternary extract compounding and antioxidant Capacity determination
2.2.1 Sample compounding
The cortex moutan extract obtained in 1.2, the red ginseng rootlet extract obtained in 1.3 and the ganoderma lucidum fruiting body extract obtained in 1.4 are mixed and compounded according to the proportion shown in Table 6, and DPPH free radical scavenging capacity is used as an index for evaluating the compounding effect. Table 6 shows the results of the complex proportion design by the uniform design formula design method, and the complex proportion was analyzed by calculating the degree of synergy and the equivalent surface model in parallel.
TABLE 6 Compound Table (volume fraction) for each single extracted sample
2.2.2 Determination of the DPPH radical scavenging Capacity of the Compound sample
And 2.1.1.
2.2.3 Complex sample synergy calculation
Some compounds have weaker activity in a single state and have stronger antioxidation effect in a mixed state, namely positive synergistic effect, namely synergistic effect for short, exists among the components. Such as VE and VC. The analysis method of the antioxidant synergistic effect at present is mainly used for researching pharmacodynamics in the medicine field, and comprises a simple effect addition model, an independent model, an isoradiometric analysis method and a response surface method. The method is used for analyzing the synergy degree of the compound sample.
The DPPH radical scavenging ability measurement IC50 values of the samples described in 2.1.1 and 2.2.2 are expressed as the amount of VC with equal effect. The Theoretical Antioxidant Capacity (TAC) of the composite sample is related to the single antioxidant capacity, and the calculation formula is as follows:
TAC＝Wa×Ta+Wb×Tb+Wc×TC
In the formula, T a、Tb、TC represents antioxidant capacity (TE) of the same fixed dose of cortex moutan extract, ganoderma lucidum fruiting body extract and Ginseng radix Rubri extract, and W a、Wb、Wc represents weight (%) of antioxidant capacity of equivalent dose of single component of cortex moutan extract, ganoderma lucidum fruiting body extract and Ginseng radix Rubri extract in the compound sample.
The Synergistic Effect (SE) is the ratio of the actual effect to the theoretical effect.
Synergistic effects are exhibited when SE is greater than 1; SE is antagonistic when less than 1.
And independent sample T-test using SPSS 26.
2.2.4 Equivalent surface construction
The equivalent surface was constructed with reference to the concentration sum (Concentration Addition, CA) model in the chemical combination toxicity model. Mathematically, the CA model is expressed as:
where n is the component number of the mixture, C i is the concentration of component i in the mixture that produces an effect of x%, and EC x,i is the concentration of component i alone that causes an effect of x%.
After the construction of the equivalent surface is completed, multiplying the proportion of each compound sample by the IC50 value of the corresponding sample to obtain the corresponding scattered point coordinates of each sample. On both sides of the plane, one side including the origin of the coordinate system is defined as the lower plane, and the other side is defined as the upper plane. If the data points are below the plane, the compound sample is shown to generate a synergistic effect; the data points represent a compound sample composite CA model on a plane; the data points represent the antagonism on a plane.
2.2.5 Data statistics
All experiments were repeated at least three times, IC50 values were measured as a linear fit, and the Theoretical (TAC) and actual (EAC) values were analyzed for significance using independent sample T-test, p <0.05 indicated a statistical difference, p <0.01 indicated a significant statistical difference, and p <0.001 indicated a very significant statistical difference.
2.2.6 Determination of elastase inhibition Capacity of Compound sample
And 2.1.3.
2.3 Experimental results
2.3.1 Activity measurement of Single oil extract
The clearance of DPPH radicals was linearly fitted according to the concentrations of the samples to obtain IC50 values of the samples. The IC50 values for samples G1#, G2#, and G3# are summarized in Table 7. The DPPH free radical scavenging ability of three single extracted samples is relatively close, the effect is obvious, and the effect is better by taking the DPPH free radical scavenging ability as a compound assessment index.
The clearance of ABTS radicals was linearly fitted according to the different concentrations of the sample to obtain the IC50 values of the sample. The IC50 values for samples G1#, G2#, and G3# are summarized in Table 7. ABTS free radical reducing ability of cortex moutan extract > Ginseng radix extract > Ganoderma extract. However, the three have lower ABTS radical reduction capability than DPPH radical scavenging capability.
Because the elastase measurement system is a water-soluble system, the oil system cannot effectively detect, 95% ethanol is used for replacing oil, the same raw materials are extracted by the same extraction process, and H1#, H2#, H3# samples are obtained, so that the elastase inhibition capacity of the G1#, G2#, G3# samples is simulated and detected equivalently.
Linear fitting was performed on the inhibition rate of elastase according to the different concentrations of the sample to obtain IC50 values of the sample. The IC50 values for h1#, h2#, h3# samples are summarized in table 7. The elastase inhibition ability of Ganoderma lucidum extract > cortex moutan extract > Ginseng radix extract.
Table 7G# sample antioxidant capacity IC50 value
/>
A comparison of the activities of extracts of a single plant is shown in FIG. 3. In summary, the activities of three oil extracts of ganoderma lucidum, ginseng and peony are as follows: DPPH radical scavenging ability > ABTS radical reducing ability > elastase inhibiting ability. Specifically, the DPPH free radical scavenging ability of the three oil extracts is similar; ABTS radical reducing ability: peony extract > ginseng extract > ganoderma lucidum extract; elastase inhibition ability: ganoderma lucidum extract > peony extract > ginseng extract. Furthermore, the results of the elastase inhibition ability measured for each sample extracted with 95% ethanol do not fully represent the elastase inhibition ability of each oil extract. Therefore, the three oil extracts have optimal DPPH free radical scavenging effect, and finally the DPPH free radical scavenging capacity is measured as an antioxidant capacity evaluation index of the compound sample to perform optimal formula optimization. In FIG. 3, the left Y-axis (0.55-0.75) is the DPPH radical scavenger IC50 value of D-G1#, D-G2#, D-G3#, i.e., to the left of the dashed line; the right Y-axis (0-160) is the ABTS radical scavenger, elastase inhibition IC50 value for A-G1#, A-G2#, A-G3#, H1#, H2#. D-G1# represents DPPH radical scavenging ability of peony extract; A-G1# represents the ABTS free radical reducing ability of peony extract; h1# represents elastase inhibitory ability of peony extract. G2# and H2# are Ginseng radix extract, and G3# and H2# are Ganoderma extract.
2.3.2 DPPH free radical scavenging ability of the Complex oil extract
The composition is characterized in that G1# is a tree peony bark extract, G2# is a red ginseng rootlet extract, G3# is a ganoderma lucidum fruit body extract, from the view of the IC50 value of DPPH free radical scavenging capacity of each compound sample and a single sample, the IC50 value of DPPH free radical scavenging capacity of each compound sample is lower than that of each single sample, which indicates that the DPPH free radical scavenging capacity of the compound sample is obviously improved, and that the three combinations have a synergistic effect compared with the single oil extract; the DPPH free radical scavenging ability IC50 values of the K4# and K5# samples are slightly lower than that of each single sample but are not much different; the DPPH free radical scavenging capacity IC50 values of the K6# and K7# samples are slightly higher than that of each single sample. The graph of the DPPH radical scavenging capacity IC50 versus the graph of fig. 4 is shown in fig. 4: p <0.001 represents a very significant statistical difference; * ***: p < 0.0001 was more pronounced than the statistical difference of P < 0.001.
2.3.3 Complex sample synergy calculation
The VC equivalent values of the single sample and the compound sample are shown in fig. 5, and in fig. 5, the VC equivalent values of the compound sample and the single sample are shown in fig. 5: p <0.001 indicates a very significant statistical difference. Theoretical values and synergy were calculated and the results are shown in table 8. Wherein the highest degree of synergy of K2# is 2.535; k1# synergy was 2.236, which differed by 0.299, and both were significantly different.
Table 8 summary of theoretical values and synergy of samples
Note that: * : p <0.05, indicating a statistical difference; * *: p <0.01, indicating significant statistical differences; * **: p <0.001 indicates a very significant statistical difference.
2.3.4 Equivalent facets
The equivalent surface equation is constructed according to the CA model as follows:
Wherein x is the G1 sample concentration (%); y is G2 sample concentration (%); z is G3 sample concentration (%).
The equivalent surfaces are shown in fig. 6-8. On a plane formed by points G1#, G2#, and G3#, the point of the compound sample below the plane is denoted as a synergistic effect, the point above the plane is denoted as an antagonistic effect, and the point falling within the plane is denoted as a summation effect.
As shown in the following figures, k1# -k5# shows a synergistic effect below the plane, k6# is antagonism, and k7# is additive effect. Approximately the same as the synergy calculation result. K1# is similar to K2# in color, and the degree of synergy is comparable.
Therefore, in all the combinations, the highest degree of synergy of the combination K2# is 2.535, and the corresponding combination proportion is 50% of peony oil extract, 45.8% of ginseng oil extract and 4.17% of ganoderma lucidum oil extract; the combination k1# synergy, second, was 2.236, which was 0.299 different from k2# (k1# and k2# were not statistically different in synergy). K1# and k2# have significant differences in antioxidant capacity compared to a single sample.
2.3.5 Elastase inhibition ability of the Compound sample
The results of testing the elastase inhibitory abilities of the k1# and k2# samples show that the elastase inhibitory abilities of the k1# and k2# samples have significant differences in fig. 9. The difference in elastase inhibitory abilities between the k1# and k2# samples was large, and the elastase inhibitory ability between the k1# samples was large, as shown in fig. 9: p <0.05, indicating a statistical difference; * *: p <0.01 indicates significant statistical differences. The elastase inhibitory capacity of each single sample versus the formulated sample versus, for example, that shown in fig. 10, it can be seen that there is no significant difference in the elastase inhibitory capacity of the formulated sample versus the single sample.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (3)
1. A compound extract, which is characterized by comprising cortex moutan extract, ginseng extract and ganoderma lucidum extract:
The volume ratio of the cortex moutan extract, the ginseng extract and the ganoderma lucidum extract is 71.1:12:16.8: or (b)
The volume ratio of the cortex moutan extract, the ginseng extract and the ganoderma lucidum extract is 50:45.8:4.17;
The preparation method of the cortex moutan extract comprises the following steps: ultrasonic extracting cortex moutan with an extracting solvent at 20-30deg.C with a feed-liquid ratio of 1:15 for 60 min, filtering with filter paper to remove residue, steaming to remove ethanol, and mixing oil to compensate for weight loss in each step;
The extraction solvent consists of 25wt% of red rice oil, 25wt% of ethanol and 50wt% of GTCC;
the mixed grease is red rice oil and GTCC; the mass ratio of the red rice oil to the GTCC is 1:2;
the frequency of the ultrasonic wave is 25.6kHz;
The ginseng extract is red ginseng rootlet extract;
The preparation method of the ginseng extract comprises the following steps: ultrasonic extracting ginseng by using an extracting solvent at the temperature of 20-30 ℃ and the feed-liquid ratio of 1:15 for 60min, filtering residues by filter paper, removing ethanol by rotary evaporation, and adding mixed grease to compensate the weight loss in each step;
The extraction solvent consists of 25wt% of red rice oil, 25wt% of ethanol and 50wt% of GTCC;
the mixed grease is red rice oil and GTCC; the mass ratio of the red rice oil to the GTCC is 1:2;
the frequency of the ultrasonic wave is 25.6kHz;
the Ganoderma extract is Ganoderma lucidum fruiting body extract;
the preparation method of the ganoderma lucidum extract comprises the following steps: ultrasonically extracting ganoderma lucidum with an extraction solvent at a temperature of 20-30 ℃ and a feed-liquid ratio of 1:15 for 60min, filtering residues with filter paper, removing ethanol by rotary evaporation, and adding mixed grease to compensate the weight loss in each step;
The extraction solvent consists of 25wt% of red rice oil, 25wt% of ethanol and 50wt% of GTCC;
the mixed grease is red rice oil and GTCC; the mass ratio of the red rice oil to the GTCC is 1:2;
The frequency of the ultrasound was 25.6kHz.
2. Use of a complex extract according to claim 1 for the preparation of an antioxidant product.
3. Cosmetic comprising the complex extract of claim 1, and cosmetically acceptable adjuvants.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211647277.4A CN115957164B (en) | 2022-12-21 | 2022-12-21 | Composite extract, application thereof and cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211647277.4A CN115957164B (en) | 2022-12-21 | 2022-12-21 | Composite extract, application thereof and cosmetics |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115957164A CN115957164A (en) | 2023-04-14 |
| CN115957164B true CN115957164B (en) | 2024-04-19 |
Family
ID=87354298
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211647277.4A Active CN115957164B (en) | 2022-12-21 | 2022-12-21 | Composite extract, application thereof and cosmetics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115957164B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150109031A (en) * | 2014-03-19 | 2015-10-01 | 김형길 | Cosmetic composition and a method of manufacturing |
| CN107184959A (en) * | 2017-05-07 | 2017-09-22 | 湖北民族学院 | A kind of honeysuckle frost and preparation method thereof |
| CN109512734A (en) * | 2017-11-24 | 2019-03-26 | 北京东方淼森生物科技有限公司 | Have effects that restore exterior-applied plant extract, cosmetics of elasticity of skin and gloss and preparation method thereof |
| CN110742843A (en) * | 2019-11-20 | 2020-02-04 | 上海华伊美化妆品有限公司 | Anti-aging traditional Chinese medicine extract of ginseng and glossy ganoderma as well as preparation method and application thereof |
| CN112137942A (en) * | 2020-07-09 | 2020-12-29 | 广州环亚化妆品科技有限公司 | Anti-aging traditional Chinese medicine composition and application thereof |
| KR102304035B1 (en) * | 2021-05-10 | 2021-09-23 | 주식회사 봉황천종삼중앙연구소 | Composition containing red ginseng ginsenoside for mask coating using functional materials having immunity improvement and antimicrobial effect and functional mask using the same |
| CN114886826A (en) * | 2022-05-26 | 2022-08-12 | 江苏巴帝恩生物科技有限公司 | Preparation method of plant firming compound |
-
2022
- 2022-12-21 CN CN202211647277.4A patent/CN115957164B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150109031A (en) * | 2014-03-19 | 2015-10-01 | 김형길 | Cosmetic composition and a method of manufacturing |
| CN107184959A (en) * | 2017-05-07 | 2017-09-22 | 湖北民族学院 | A kind of honeysuckle frost and preparation method thereof |
| CN109512734A (en) * | 2017-11-24 | 2019-03-26 | 北京东方淼森生物科技有限公司 | Have effects that restore exterior-applied plant extract, cosmetics of elasticity of skin and gloss and preparation method thereof |
| CN110742843A (en) * | 2019-11-20 | 2020-02-04 | 上海华伊美化妆品有限公司 | Anti-aging traditional Chinese medicine extract of ginseng and glossy ganoderma as well as preparation method and application thereof |
| CN112137942A (en) * | 2020-07-09 | 2020-12-29 | 广州环亚化妆品科技有限公司 | Anti-aging traditional Chinese medicine composition and application thereof |
| KR102304035B1 (en) * | 2021-05-10 | 2021-09-23 | 주식회사 봉황천종삼중앙연구소 | Composition containing red ginseng ginsenoside for mask coating using functional materials having immunity improvement and antimicrobial effect and functional mask using the same |
| CN114886826A (en) * | 2022-05-26 | 2022-08-12 | 江苏巴帝恩生物科技有限公司 | Preparation method of plant firming compound |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115957164A (en) | 2023-04-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mondal et al. | In vitro antioxidant activity of Diospyros malabarica Kostel bark | |
| Barreca et al. | Evaluation of the antioxidant and cytoprotective properties of the exotic fruit Annona cherimola Mill.(Annonaceae) | |
| US9884006B2 (en) | Coffee cherry cosmetic composition and methods | |
| He et al. | Analysis of phenolic compounds in Chinese olive (Canarium album L.) fruit by RPHPLC–DAD–ESI–MS | |
| Hanachi et al. | Using HPLC to determination the composition and antioxidant activity of Berberis vulgaris | |
| Onkar et al. | Evaluation of Antioxidant activity of traditional formulation Giloy satva and hydroalcoholic extract of the Curculigo orchioides Gaertn | |
| CN102940597B (en) | Natural compound botanical antioxidant, as well as preparation method and application thereof in cosmetics | |
| KR101682552B1 (en) | Antioxidant composition comprising red ginseng extracts and berrylike mixtures | |
| MXPA04001115A (en) | Artichoke leaf extracts. | |
| CN115531272B (en) | Antioxidant composition derived from alpine plants and preparation method and application thereof | |
| Ehiobu et al. | Phytochemical content and antioxidant potential of leaf extracts of Citrus limon (L.) Osbeck collected in the Eastern Cape Province, South Africa | |
| Kang et al. | Main anthraquinone components in Aloe vera and their inhibitory effects on the formation of advanced glycation end‐products | |
| Rafael et al. | Topical anti-inflammatory plant species: Bioactivity of Bryonia dioica, Tamus communis and Lonicera periclymenum fruits | |
| Ding et al. | Antioxidant activity in vitro guided screening and identification of flavonoids antioxidants in the extract from tetrastigma hemsleyanum diels et gilg | |
| CN110859788B (en) | Tanaka composition and preparation method and application thereof | |
| CN115957164B (en) | Composite extract, application thereof and cosmetics | |
| CN106333972B (en) | Antifatigue effective part extract of okra and its preparation method and application | |
| Nawrot-Hadzik et al. | Analysis of antioxidant polyphenols in loquat leaves using HPLC-based activity profiling | |
| Xu et al. | Analysis of trace elements in Chinese therapeutic foods and herbs | |
| KR100722955B1 (en) | The herbal composition having the skin antiaging and antiwrinkling function | |
| KR20220095075A (en) | Anti-inflammation composition, Health functional food containing the same and Pharmaceutical composition containing the same | |
| CN113057917A (en) | Abelmoschus manihot anti-aging composition and preparation method and application thereof | |
| Li et al. | Formation of Amadori compounds in LIGAO (concentrated pear juice) processing and the effects of Fru-Asp on cough relief and lung moisturization in mice | |
| KR101429049B1 (en) | A Composition for Improving Beauty of Skin Using Red Ginseng Extraxts | |
| CN108030813A (en) | A kind of clarification process and method of quality control of the pharmaceutical composition with delay skin aging |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |