KR100722955B1 - The herbal composition having the skin antiaging and antiwrinkling function - Google Patents

Abstract

The present invention relates to a herbal composition having an anti-aging and anti-wrinkle function.

Herbal medicine composition having the anti-aging and anti-wrinkle function of the present invention, the extract of the upper leaf extracted with methanol, the Goji berry extract extracted with water, and the pottery extract extracted with methanol, respectively, 40 ~ 70: 5 ~ 20: It consists of preparing a herbal composition by mixing at a weight ratio of 10 to 50.

According to the present invention, a herbal composition having no side effects and excellent efficacy is provided, and an herbal composition having excellent elastase inhibitory activity, SOD activity and antioxidant activity, and having anti-aging and anti-wrinkle function is provided.

Upper lobe, wolfberry, cabbage, skin aging, anti wrinkle

Description

Herbal composition with anti-aging and anti-wrinkle function {THE HERBAL COMPOSITION HAVING THE SKIN ANTIAGING AND ANTIWRINKLING FUNCTION}

1 is a manufacturing process of the herbal medicine composition having the anti-aging and anti-wrinkle function of the present invention

The present invention relates to a herbal composition having an anti-aging and anti-wrinkle function.

Skin aging is inferior to the elasticity and shine of the skin, pigmentation of the skin such as blemishes, freckles are deposited, wrinkles appear.

This skin aging phenomenon is caused by loss of biocombination water in skin cells, structural changes in the stratum corneum, decrease of differentiation of epidermal cells, deterioration of biosynthetic function of proteins and intercellular materials for dermal fibroblasts. In other words, the skin tissue is thinned by this action appears in the form of wrinkles.

In the dermal tissue of the skin, collagen and elastin, which is related to the elasticity of the skin, form a network structure. As this network structure is broken, elastin is broken down by elastinase and the skin is treated. As it becomes thinner and wrinkles occur, endogenous skin aging occurs.

Therefore, skin aging can be suppressed by lowering the activity of elastinase, one of the main causes of skin aging.

In addition, one of the causes of skin aging is the overproduction of free radicals.

Free radicals are constantly produced in various metabolic processes of various cells in the body, and due to various pollution caused by the highly developed industrial society, more than the right amount of free radicals are generated in our body, which is the cause of various diseases and aging May cause skin wrinkles.

SOD (Superoxide Dismutase) is an enzyme that removes free radicals. It removes when there are more free radicals in the body and plays a role in preventing or treating diseases caused by oxidation of free radicals.

In addition, antioxidant activity also plays a role in removing the active oxygen.

When the free radicals generated in the body destroy other substances in the body to stabilize themselves, the biological component breaks its structure and damages the cells in the body, which in turn degrades or loses its function. Get up.

To date, many anti-aging agents have been developed and used, but their efficacy is weak and easily oxidized by air, so there is a low sustainability, a high price problem, and there is a problem of low safety during long-term use.

However, cosmetic compositions made of chemicals have side effects, and studies have been made to develop high anti-aging and anti-wrinkle substances even when used for a long time using natural substances.

Korean Patent Publication No. 10-2005-0002581 (preservatives for preventing skin aging and acne improvement using herbal medicines) includes licorice, gold silver, seooh, pogongyoung, nohee, yulpi, white paper, golden, bokyeong, three hundred seconds, rice bran, yellow lotus It discloses about the cosmetics manufactured by mixing powdered extract in a fixed ratio.

In addition, Korean Patent Publication No. 10-0361433 (cosmetic composition for preventing skin aging containing ginseng extract), after extracting ginseng with ethanol, ethanol insoluble residue was filtered through diatomaceous earth, filtered with diatomaceous earth, and then obtained by precipitation with ethanol to precipitate Discloses a cosmetic composition for preventing skin aging, characterized in that it is contained in an amount of 10 ^-8 to 10 ¹ % by weight based on the total weight of the composition.

However, the composition using the conventional natural materials as described above has a problem in that the effect is somewhat reduced, or due to the toxicity of the natural material itself when used for a long time.

In order to solve the above problems, an object of the present invention is to provide a herbal composition having anti-aging and anti-wrinkle functions by using the upper leaf, goji berry, and indoin.

In addition, the herbal composition of the present invention is an herbal composition having excellent elastase inhibitory activity, SOD activity, and antioxidant activity to prevent skin aging and anti-wrinkle function, and this herbal composition is added to a general cosmetic composition and thus has no toxicity. There is no purpose, and to provide a cosmetic composition with high safety even in long-term use.

The present invention relates to a herbal composition having an anti-aging and anti-wrinkle function.

Herbal medicine composition having anti-aging and anti-wrinkle function of the present invention, 100% methanol concentration in the upper leaf (mulberry leaf) powder 20 times the weight of the upper leaf powder, heated to 50 ℃ for 12 hours, and then centrifuged After separation and extraction, the extract was filtered with a filter and concentrated at 50 ° C. using a reduced pressure concentrator to prepare an extract of the upper leaf, and water was added to the goji powder to be 20 times the weight of the goji powder. After heating for 12 hours and extracting by centrifugation, the extract was filtered with a filter and concentrated at 30 ° C. using a vacuum concentrator to produce goji extract. 20 times the weight of the powder, heated to 40 ℃ for 18 hours, centrifuged and extracted, the extract was filtered with a filter and 40 using a vacuum condenser 40 to 70: 5 to 20: 10, respectively, which were prepared at the third step, the upper leaf extract prepared at the first step, the goji berry extract prepared at the second step, and the seed extract prepared at the third step. It consists of a fourth process consisting of preparing a herbal composition by mixing at a weight ratio of ~ 50.

The inventors of the present invention using the herbal medicines having the anti-aging and anti-wrinkle effect among the herbal medicines that do not have any side effects, find the most suitable extraction method and blending ratio to produce the most effective herbal anti-aging and anti-wrinkle composition To this end, the present invention has been completed while undergoing many trials and errors.

The herbal medicines used in the present invention are upper leaf, goji berry, cabbage.

Morus alba refers to the leaves of mulberry, collectively called deciduous tree or shrub belonging to the mulberry family.

This upper lobe has the effect of preventing and treating diabetes, and is good for high blood pressure, and contains a significant amount of anti-cancer quercetin and isoqueresetin, and is effective in improving liver function, triglyceride breakdown, diet, heavy metal excretion, and blood circulation. Known.

Lycium chinense is a deciduous tree of the deciduous shrub belonging to the branch family, and contains alkaloids, vitamins, and trace elements. It is tonic, blood pressure lowering, blood sugar lowering, anti-interstitial and liver function. It is known that there is a protective action, such as anorexia, constipation, acne is known to be good.

Prunus persica refers to dried seeds of the ripe fruit of the peach tree, a rose family. It is bitter, sweet and has no poison, and acts on the heart, liver and large intestine.

In addition, it is active in the movement of blood and eliminates blood, so it is mainly used for menstrual irregularities, menstrual pain, itchy skin, dry or blemishes and freckle, etc., is known to be good for constipation and diarrhea.

The upper leaf and the vine were extracted with methanol, and the wolfberry was extracted with water.

In addition, elastase inhibitory activity and SOD activity when the herbal extracts were prepared by mixing the mixing ratios of 40 ~ 70: 5 ~ 20: 10 ~ 50 by weight ratio when mixing the extracts of the leaf, goji berry extract, It was found that the antioxidant activity was the best, and the effect was great.

Hereinafter, the manufacturing process of the herbal medicine composition having the anti-aging and anti-wrinkle function of the present invention will be described in detail.

<Manufacturing process of herbal medicine composition with anti-aging and anti-wrinkle function>

1. First Process: Preparation of Leaf Extract

The leaf powder of mulberry leaves is purchased commercially.

Methanol (100%) is added to the prepared upper leaf powder 20 times the weight of the upper leaf.

It is heated to 50 ° C. for 12 hours and then extracted by centrifugation.

The extract was filtered with a filter, and then concentrated at 50 ° C. using a vacuum condenser to prepare an extract of the upper leaves.

2. Second Process: Preparation of Goji Extract

Goji berries are commercially available, washed and prepared.

After the prepared wolfberry is pulverized into powder, water is added to the wolfberry powder to be 20 times the weight of the wolfberry powder.

It is heated to 30 ° C. for 12 hours and then extracted by centrifugation.

The extract was filtered with a filter, and then concentrated at 30 ° C. using a vacuum concentrator to prepare goji-ja extract.

3. Third Process: Preparation of Doin Extract

Doin is purchased from the market, washed, and prepared.

The prepared phosphorus is pulverized to prepare a powder, and methanol (100%) is added to the phosphorus powder so that it is 20 times the weight of the phosphorus powder.

It is heated to 40 ° C. for 18 hours and then extracted by centrifugation.

The extract was filtered with a filter, and then concentrated at 40 ° C. using a vacuum concentrator to prepare a ceramic extract.

4. The fourth step: mixing

The herb extract prepared in the first step and the goji berry extract prepared in the second step and the porcelain extract prepared in the third step are mixed at a weight ratio of 40 to 70: 5 to 20: 10 to 50, respectively, to prepare the herbal composition. Manufacture.

The herbal composition of the present invention prepared by the above method has a high inhibitory activity of elastinase, excellent SOD activity and antioxidant activity, and proved to be a material having anti-aging and anti-wrinkle function.

In addition, the herbal composition of the present invention can be added to a conventional cosmetic composition to prepare a cosmetic composition having the function of preventing skin aging and suppressing wrinkles.

When the herbal composition of the present invention is added to the cosmetic composition, it can be added by adjusting the amount of addition according to the use of each cosmetic composition.

Hereinafter, the herbal medicine composition having the anti-aging and anti-wrinkle function of the present invention will be described in more detail through Examples and Experimental Examples, but these are not intended to limit the scope of the present invention.

<Example 1> Preparation of herbal medicine composition having anti-aging and anti-wrinkle function 1

The leaves of mulberry (mulberry leaves) were purchased from the market and prepared.

200 g of methanol (100%) was added to 10 g of the prepared upper leaf powder, heated at 50 ° C. for 12 hours, and extracted by centrifugation.

The extract was filtered with a filter (Watman No. 1), and then concentrated at 50 ° C. using a reduced pressure concentrator to prepare an upper leaf extract.

Goji berries were purchased commercially, washed and prepared, and then ground to a powder.

200 g of water was added to 10 g of the prepared goji berry powder, heated at 30 ° C. for 12 hours, and then extracted by centrifugation.

The extract was filtered with a filter (Watman No. 1), and then concentrated at 30 ° C. using a vacuum concentrator to prepare goji extract.

Doin (radiation seed) was purchased commercially and prepared by washing.

The prepared phosphorus was ground to prepare a powder, and 200 g of methanol (100%) was added to 10 g of the phosphorus powder and heated at 40 ° C. for 18 hours.

The heated material was extracted by centrifugation, and the extract was filtered with a filter (Watman No. 1).

The filtrate was concentrated at 40 ° C. using a vacuum concentrator to prepare a ceramic extract.

The herbal extract composition of the present invention was prepared by mixing 40 mg of the lettuce extract prepared above, 20 mg of goji berry extract, and 40 mg of phosphorus extract.

<Example 2> Preparation of herbal medicine composition having anti-aging and anti-wrinkle function 2

In the same manner as in Example 1 of the present invention was prepared by preparing the leaf extract, goji berry extract, doin extract.

The herbal extract composition of the present invention was prepared by mixing 55 mg of the prepared leaf extract, 17 mg of Goji extract, and 28 mg of Doin extract.

<Example 3> Preparation of herbal medicine composition having anti-aging and anti-wrinkle function 3

In the same manner as in Example 1 of the present invention was prepared by preparing the leaf extract, goji berry extract, doin extract.

The herbal extract composition of the present invention was prepared by mixing 45 mg of the prepared leaf extract, 10 mg of Goji berry extract, and 45 mg of Doin extract.

<Example 4> Preparation of herbal medicine composition having anti-aging and anti-wrinkle function 4

In the same manner as in Example 1 of the present invention was prepared by preparing the leaf extract, goji berry extract, doin extract.

The herbal extract composition of the present invention was prepared by mixing 45 mg of the prepared leaf extract, 5 mg of Goji berry extract, and 50 mg of Doin extract.

<Example 5> Preparation of herbal medicine composition having anti-aging and anti-wrinkle function 5

In the same manner as in Example 1 of the present invention was prepared by preparing the leaf extract, goji berry extract, doin extract.

The herbal extract composition of the present invention was prepared by mixing 70 mg of the prepared leaf extract, 20 mg of Goji berry extract, and 10 mg of Doin extract.

<Example 6> Preparation of the cosmetic composition containing the herbal composition of the present invention 1

The herbal composition prepared by the method of Example 2 of the present invention was prepared.

A cosmetic lotion composition prepared by a conventional method was prepared.

50 ml of the herbal medicine composition of the present invention was mixed with 1,000 ml of the prepared cosmetic composition to prepare a cosmetic composition having an anti-aging and anti-wrinkle function containing the herbal medicine composition of the present invention.

<Example 7> Preparation of the cosmetic composition containing the herbal composition of the present invention 2

A herbal composition prepared by the method of Example 4 of the present invention was prepared.

An eye cream composition prepared by a conventional method was prepared.

50 g of the herbal medicine composition of the present invention was mixed with 100 g of the prepared eye cream composition to prepare a cosmetic composition having an anti-aging and anti-wrinkle function containing the herbal medicine composition of the present invention.

<Example 8> Preparation of the cosmetic composition containing the herbal composition of the present invention 3

The herbal composition prepared by the method of Example 5 of the present invention was prepared.

An essence composition prepared by a conventional method was prepared.

50 ml of the herbal composition of the present invention was mixed with 1,000 ml of the prepared essence composition to prepare a cosmetic composition having an anti-aging and anti-wrinkle function containing the herbal composition of the present invention.

Experimental Example 1 Enzyme Activity Measurement Experiment

In order to prove the effect of each extract constituting the herbal composition of the present invention, the experiment to measure the elastase inhibitory activity, SOD activity, and antioxidant activity, respectively.

1. Experimental Materials

In the same manner as in Example 1 of the present invention was prepared by preparing the upper leaf extract, goji berry extract, the extract of the pottery.

2. Experimental method

Elastase inhibitory activity was measured using azocasein as follows.

That is, 0.005 g of azocasein was dissolved in 0.2 M Tris-CI buffer (pH 8.0) and used as a substrate.

In this experiment, 1 unit / 100 μl elastase and 0.8 mM N-Suc- (Ala) 3-ρ-nitroanilide were added to 0.2 M Tris-CI buffer (pH 8.0) for 20 minutes at 25 ° C. Absorbance was measured at nm to quantify the amount of ρ-nitroanilide produced from the substrate.

One unit of elastase inhibitory activity was defined as the amount of the enzyme of elastase that produced ρ-nitroanilide of 1 Чmol per minute.

In addition, inhibitory activity is computed from the following formula.

[Elastase inhibitory activity (%) = (1-B / A) × 100]

     * A: Elastase of untreated group

     * B: Elastase of treatment group

SOD activity was measured by Cuord and Fridovich's method.

Into the reaction mixture (50 ЧM xanthine, 0.1 mM NaOH, 20 ЧM cytochrome C, 44 mM potassium phosphate buffer (pH 7.8), 0.1 mM EDTA), 300 ㎍ natural product and 1 ㎍ SOD were added and xanthine oxidase was added. Absorbance change was measured at 550 nm.

The amount of xanthine used was adjusted to O.D units / min 0.025 ± 0.004 so as to be sufficient to reduce Ferricicytochrome C (Ferricytochrome C).

One unit of enzyme was defined as the amount of SOD capable of reducing cytochrome C by 50% under the above conditions (more than 100% means an activation reaction and less than 100% means an inhibitory reaction. ).

Antioxidant activity was measured by methods such as Blois and Lee using reducing power of DPPH.

0.8 ml of DPPH solution (dissolved 12.5 mg of DPPH in 100 ml of ethanol) was added to 0.2 ml of the sample, followed by reaction for 10 minutes, and the absorbance was measured at 525 nm. The activity was compared with the sample-free control.

Antioxidant activity was obtained from the following equation.

[Electron Donation Ability (%) = {1-(A-B / C)} × 100]

At this time, C was measured for absorbance using ethanol as a control, A was measured after reacting the sample and DPPH, B was used as distilled water instead of ethanol as the absorbance of the sample.

3. Experimental Results

As a result of measuring the elastase inhibitory activity, SOD activity, and antioxidant activity of the lettuce extract, goji berry extract, and cabbage extract by the above method were as shown in Table 1 below.

<Table 1> Enzyme activity measurement results for each plant extract

division Elastase Inhibitory Activity SOD activity Antioxidant activity (%) Morus alba 58.0% 49.5 μg / ml 26.3% Goji berry extract ( Lycium chinense ) 0 197.0 μg / ml 18.0% Prunus persica 0 0 98.0%

As shown in Table 1 above, as a result of measuring the respective enzyme activity for each extract as a constituent of the herbal medicine composition of the present invention, the extract of the upper leaf, the activity of elastase inhibitory activity is 58.0%, SOD activity is good And antioxidant activity was found to be very poor.

In addition, goji berry extract had a high SOD activity of 197.0 ㎍ / ㎖, but showed no elastase inhibitory activity, and also showed very low antioxidant activity.

Also, the phosphorus extract showed no elastase inhibitory activity and no SOD activity, and only the antioxidant activity was 98.0%.

In other words, each extract constituting the herbal composition of the present invention was good activity for only one enzyme activity, the rest was not found to be at all or extremely low activity.

Experimental Example 2 Activity Test of Herbal Medicine Composition of the Present Invention

The herbal compositions of Examples 1 to 5 of the present invention were prepared.

Each herbal composition prepared by varying the mixing ratio was tested in the same manner as in Experiment 1 to measure the elastase inhibitory activity, SOD activity, antioxidant activity.

Enzyme activity measurement results are shown in Table 2 below.

<Table 2> Enzyme activity measurement results for the herbal composition of the present invention

division Leaf extract Goji Extract Doin Extract Elastase inhibitory activity SOD activity Antioxidant activity Example 1 40% 20% 40% 24.0 125 50.2 Example 2 55% 17% 28% 34.5 120 31.0 Example 3 45% 10% 45% 58.5 163 51.0 Example 4 45% 5% 50% 60.1 184 62.0 Example 5 70% 20% 10% 68.2 214 98.0

As shown in Table 2, the results of measuring the enzyme activity of the herbal composition of the present invention, each extract was found to be evenly excellent even for the remaining activity was not active or extremely low.

In particular, it was confirmed that the herbal composition of Example 5 shows an excellent effect in all enzyme activities.

Therefore, the herbal composition of the present invention is prepared by mixing the leaf extract with 40 to 70% of the total weight, goji berry extract 5 to 20% of the total weight, the indo extract at a ratio of 10 to 50% of the total weight It is confirmed that the inhibitory activity, SOD activity, and antioxidant activity are all excellent, and that the herbal composition prepared as described above was confirmed as a herbal composition with anti-wrinkle and preventive function and anti-aging effect.

According to the present invention, a herbal composition having anti-aging and anti-wrinkle functions is provided by using upper leaves, goji berry, and cabbage.

In addition, the herbal composition of the present invention is an herbal composition having excellent elastase inhibitory activity, SOD activity, and antioxidant activity to prevent skin aging and anti-wrinkle function, and a cosmetic composition containing the herbal composition is provided.

Claims (3)

In herbal medicine composition, The herb extract, goji berry extract and sperm extract are mixed with herbs in the weight ratio of 40 ~ 70: 5 ~ 20: 10 ~ 50, respectively. Herbal composition with anti-aging and anti-wrinkle function. The method of claim 1, The upper leaf extract, 100% methanol is added to the upper leaf powder 20 times the weight of the upper leaf powder, heated to 50 ℃ for 12 hours, and extracted by centrifugation, the extract is filtered with a filter and concentrated under reduced pressure It was prepared by concentrating at 50 ℃ using, Gojija extract, put water in the gojija powder to 20 times the weight of the gojija powder, heated to 30 ℃ for 12 hours, and then extracted by centrifugation, the extract was filtered through a filter and reduced to 30 ℃ using a vacuum condenser Was prepared by concentration in The phosphorus extract is added to methanol at a concentration of 100% in the phosphorus powder to 20 times the weight of the phosphorus powder, heated at 40 ° C. for 18 hours, and then extracted by centrifugation. Characterized in that prepared by concentrating at 40 ℃ using, Herbal composition with anti-aging and anti-wrinkle function. The herbal composition according to claim 1 or 2, Characterized in that it is added to one of the cosmetic composition selected from the lotion, lotion, eye cream, essence, cream, pack, Herbal composition with anti-aging and anti-wrinkle function.

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