KR100604244B1 - Cosmetic composition containing Solanum Lycopersicum MILL.Extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a tomato extract. More specifically, the present invention relates to a cosmetic composition containing a tomato extract, which is excellent in moisturizing, antioxidant and whitening effects and contains a stable tomato extract. The cosmetic composition containing the tomato extract according to the present invention is characterized by containing 10.0 to 30.0% by weight of the tomato extract extracted from the mixed solvent of 1,3 butylene glycol and olive oil.

Description

Cosmetic composition containing tomato extract {Cosmetic composition containing Solanum Lycopersicum MILL.Extract}

The present invention relates to a cosmetic composition containing a tomato extract. More specifically, the present invention relates to a cosmetic composition containing tomato extract having excellent moisturizing, antioxidant and whitening effects and having formulation stability.

In general, tomatoes (Solanum Lycopersicum MILL.) Is an annual herb of dicotyledons and moss-branches. It is also known as an annual herb. Tomato fruit is about 90% water and contains vitamins A, B, C, E, K, potassium, calcium, Carotene and lycopene are many. Beta-carotene and lycopene in tomatoes are known to prevent cancer, and tomatoes are also known to play a role in relieving constipation and making elastic and fair skin. It also controls the amount of moisture in the body to suppress overeating. It is known to function to promote digestion and to promote the action of the stomach, pancreas, liver and the like. Tomatoes are known to be effective in improving the function of the large intestine, which lowers the level of cholesterol in the blood and prevents obesity. Tomatoes are known to be particularly effective in preventing vitamins from aging and preventing osteoporosis and senile dementia, especially among vitamin C. These vitamins contain anti-aging ingredients that help to rejuvenate your body, prevent osteoporosis, and vitamin C in tomatoes is more potent in inhibiting carcinogens than vitamin C in other fruits and vegetables. (Herb illustration, Ahn Deok-gyun, 1998, 699p), also promotes metabolism and regulates the amount of urine, and is good for febrile diseases such as hepatitis (熱 性病). In addition, it is described in agreement that the customary skin disease is effective by drinking a little water boiled tomato roots, stems, and leaves, washing the body frequently with this water, and drinking a little foreign body.

As described above, if a person ingests a tomato having a lot of nutrients will be greatly helped for health. Attempts have been made to apply such tomatoes to cosmetics. For example, Korean Patent Publication No. 10-0280030, which can be obtained by acid-alcohol extraction from a cell culture of tomato (Lycopersicum esculentum) cells, is rich in hydroxypurorine, which is more than 17%, produced from tomato. It describes a method for producing glycoproteins (Hyproproline-rich glycoproteins, HPRGs) and a cosmetic composition comprising glycoproteins as an active ingredient. However, what is described in the patent publication is that the glycoprotein is characterized by a molecular weight of 20,000 Daltons, a denaturation range of 12,000 to 38,000 and high solubility in water as measured by gel permeation and electrophoresis, and the glycoprotein is applied to an actual cosmetic. As mentioned above, the basic characteristics and specific effects that cosmetics should exhibit are not mentioned, and research on cosmetic compositions containing tomato extracts is still in its infancy.

The present invention has been made to solve the above problems, an object of the present invention is to provide a cosmetic composition containing a tomato extract that is excellent in moisturizing, antioxidant and whitening effect, and can have formulation stability.

According to the cosmetic composition containing a tomato extract according to the present invention for achieving the above object, containing 10.0 to 30.0% by weight of the tomato extract extracted from a mixed solvent of 1,3 butylene glycol and olive oil It is characterized by.

In addition, the cosmetic composition comprising a tomato extract according to the present invention is characterized in that the mixed solvent is mixed with 1,3 butylene glycol and olive oil of about 1: 1 by weight ratio.

Tomato extract of the cosmetic composition containing a tomato extract according to the present invention can be prepared by the following process using a tomato fruit.

First, the prepared tomato is physically ground. This milling process uses a known mill. For example, crushing and discharging by putting a certain amount of vegetables or fruits into a pulverizing bin, a single pulverizer pulverized by a high-speed rotating blade attached to the pulverizer, or a knife that is compressed and conveyed by a screw to rotate in contact with the pulverizing network A grinder etc. are mentioned. In this case, since the tomato extract of the present invention is to be used in cosmetics, it is necessary to prevent hygiene from being generated by foreign substances caused by abrasion such as a knife or a net.

The crushed tomatoes are extracted using a solvent mixed with 1,3-butylene glycol and olive oil. Among the solvents used in the extraction process, 3-butylene glycol is used to extract the water-soluble component, and olive oil is used to extract the oil-soluble component. In addition to the above extraction solvents, alcohols such as methanol, isopropyl alcohol, propylene glycol, ethanol, various oils, and the like can be used, but 1,3-butylene glycol has antiseptic properties and can minimize the loss of physical properties and polysaccharides. In addition, since olive oil dissolves lycopene best among the components of tomatoes, a mixed solvent of olive oil and 1,3-butylene glycol is most preferred as an extraction solvent. Such a mixed solvent functions to further enhance the moisturizing effect of the cosmetic. At this time, the mixed solvent was confirmed that the extraction of tomatoes in the solvent mixed with 1, 3- butylene glycol and olive oil in a weight ratio of about 1: 1 was confirmed. However, the weight ratio does not necessarily mean 1: 1, but may also include a mixed solvent in a weight ratio having a slight error.

The cosmetic composition containing the tomato extract according to the present invention is most preferably added 10.0 to 30.0% by weight of the tomato extract based on the total weight of the cosmetic composition. When the tomato extract is added less than 10.0% by weight, the skin moisturizing effect is reduced and there is little whitening effect, when the tomato extract exceeds 30.0% by weight, the discoloration or separation of the formulation occurs, the stability of the cosmetic composition is very low, the present invention This is because it is difficult to obtain a conventional cosmetic composition because of the high cost of the cosmetic composition, which is very economically disadvantageous and also due to the characteristics of the cosmetic composition that should contain many different raw materials.

The cosmetic composition containing the tomato extract according to the present invention prepared by the present invention inhibits free radicals that cause aging and skin cancer due to the antioxidant effect on the skin and maintains young skin, and acts on melanocytes to release melanin. It keeps your skin clean and free of blemishes by increasing the concentration of protein and albumin in your skin tissues, thereby promoting your metabolism and keeping your skin healthy.

On the other hand, the cosmetic composition containing a tomato extract according to the present invention is not particularly limited in the formulation. For example, all formulations such as skin sticky and transdermal dosage forms such as lotion, cream, pack, gel, lotion, etc. may be possible.

Hereinafter, the present invention will be described in detail through examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

EXAMPLE

Preparation of Tomato Extract

Physically grind the prepared tomato through a general grinder to make 100 g of a ground powder, and then use a mixed solvent in which 1,3-butylene glycol and olive oil are mixed at a weight ratio of about 1: 1 (50 g each) for 48 hours. Extracted and filtered through 250 mesh to obtain 170g of tomato extract. Therefore, the tomato extract used in the cosmetic composition according to the present invention will include not only the tomato component but also components of the mixed solvent used to extract it.

Preparation of tomato extract-containing cosmetic composition

Preparation Example 1 and Preparation Example 2 having a weight range according to the present invention using the tomato extract prepared above, and Comparative Preparation Examples 1 to 5 having a weight range other than the weight range according to the present invention It was prepared as a cream according to the cosmetic preparation method.

Table 1 shows the compositions of the creams used to make the creams of Preparation Example 1 and Preparation Example 2 and Comparative Preparation Examples 1 to 5, respectively, in weight percent.

TABLE 1

ingredient Content (% by weight) Preparation Example 1 Preparation Example 2 Comparative Preparation Example 1 Comparative Production Example 2 Comparative Production Example 3 Comparative Production Example 4 Comparative Production Example 5 Tomato Extract 10.0 30.0 0 5.0 8.0 32.0 40.0 Purified water Remaining amount glycerin 10.2 Xanthan Gum 0.2 antiseptic Quantity Cetyloctanoate 10.0 Polyglyceryl-3 Methyl Glucoside 2.0 Cyclomethicone 5.0 incense Hostility

The following tests were conducted to evaluate the performance of the preparations and comparative preparations prepared above.

[Test Example 1]

The skin moisturizing effect of the creams of Preparation Example 1 and Preparation Example 2 and Comparative Preparation Examples 1 to 5 prepared in Examples were tested. 35 healthy women (mean age 29.5 years old) were divided into seven groups of 35 healthy women indoors with little air flow at 22-24 degrees Celsius and 55% relative humidity. The creams of Preparation Example 1 and Preparation Example 2 and Comparative Preparation Examples 1 to 5 prepared in Example were applied to the entire face twice a day for 6 weeks, centered around the eyes of each group. By using Skicon-200 (IBS Co. Japan), the moisture content of the cheek area was measured before and after application to measure the persistence of the moisturizing effect.

Based on the test results, the average values for each test group are shown in Table 2. Here, the degree of improvement was calculated by the following equation.

[Equation 1]

Improvement = (moisture 6 weeks after coating-moisture before test) / moisture before test x 100

TABLE 2

Before the test 6 weeks after application % Improvement Preparation Example 1 645 ± 10 805 ± 10 24.81 Preparation Example 2 645 ± 10 855 ± 10 32.56 Comparative Production Example 1 645 ± 10 665 ± 10 3.10 Comparative Production Example 2 630 ± 10 685 ± 10 8.73 Comparative Production Example 3 645 ± 10 735 ± 10 13.95 Comparative Production Example 4 635 ± 10 855 ± 10 34.65 Comparative Production Example 5 630 ± 10 850 ± 10 34.92

As can be seen in Table 2, in the case of Comparative Preparation Examples 1 to 3 below the weight range of the tomato extract according to the present invention can be seen that the skin moisturizing effect is almost weak, In Preparation Example 1 and Preparation Example 2 using the tomato extract and Comparative Preparation Example 4 and Comparative Example 5 when the weight range of the tomato extract according to the present invention can be seen that the skin moisturizing effect is very excellent. However, as will be described later, Comparative Preparation Example 4 and Comparative Preparation Example 5 exceeding the weight range of the tomato extract according to the present invention is not preferable because it causes discoloration or separation symptoms to make the cosmetic unstable.

[Test Example 2]

The tyrosinase inhibitory effect of the tomato extract obtained in the above example and the whitening raw material commercially available in the cosmetics industry was measured.

The enzyme tyrosinase was extracted from mushrooms, and SIGMA T7755's was used. First, prepare a tyrosine substrate as a 0.3 mg / ml solution in distilled water, add 1.0 ml of this solution to a test tube, add 1.0 ml of potassium phosphate buffer solution (0.1M, pH 6.8) and a whitening efficacy peptide ( MCH (Melanin Cencentration Hormone) was added to each reaction solution of 0.9ml dilution step by step in the reaction solution and reacted for 10 minutes in a 37 ° C thermostat. Ethanol and water were used as the diluting solvent at 6: 4, and 0.9 ml of the comparative raw material was used instead of the whitening efficacy peptide (MCH; Melanin Cencentration Hormone) as a control. 0.1 ml of a 2,500 unit / m1 tyrosinase solution was added to the reaction solution, and the reaction solution was reacted for 10 minutes in a 37 ° C thermostat. Then, the test tube containing the reaction solution was placed in ice to stop the reaction. Absorbance was measured. The following formula was used to determine the tyrosinase inhibitory effect of each extract.

[Equation 2]

Tyrosinase Inhibition Rate (%) = 100-(Extract Reaction Absorbance / Control Absorbance) * 100

Table 3 shows the tyrosinase inhibition rate of tomato extract and arbutin.

TABLE 3

Tyrosinase inhibition rate (%) Tomato Extract Arbutin 0.0001 48.21 54.52 0.001 78.68 55.32 0.01 96.67 55.87 0.1 90.54 48.87 One 72.13 32.11 10 48.21 20.15 50 24.21 11.02

As can be seen in Table 3, the tyrosinase inhibition rate of tomato extract was 96.67% at 0.01% by weight, and 55.87% at the same concentration for arbutin widely used as a whitening cosmetic.

[Test Example 3]

In Experiment 3, a melanin production inhibitory test was performed, and the inhibitory effect of the melanin biosynthesis amount using the tomato extract was tested based on the results in Test Example 2. Human Melanocytes cultured from human tissues were cultured using primary RPMI 1640 medium at 2 * 104 / dish concentration in a 60mm culture dish after primary passage. After confirming that the cell attached to the dish, the tomato extract was treated by concentration up to 0.001% by weight to 10% by weight. After treating the tomato extract by concentration and washing it several times with pbs on the 3rd day, the cells were removed by counting with Trypsin-EDTA, counted by 2 * 105 cells, and 100 μl of 1N NaOH solution was added. The cells were treated for 12 hours at 37 degrees Celsius to completely lyse the cells. The absorbance of 500 μl of samples in which cells were completely dissolved was measured at 490 nm. As a standard indicator, 10 mg of synthetic melanin (M8601) from Sigma was dissolved in 10 ml of 1N NaOH to prepare a stock solution (1 mg / ml), followed by 700 µg / ml, 300 µg / ml, 100 µg / ml, and 70 µg / ml. Ml, 30 μg / ml, 10 μg / ml, 7 μg / ml, 3 μg / ml, 1 μg / ml, 0.1 μg / ml and 0 μg / ml were prepared and the absorbance was measured at 490 nm. As a result, melanin synthesis was reduced by up to 32% in 0.01% by weight of tomato extract.

Here, the inhibition rate (%) was calculated by the following equation, the results are shown in Table 4.

[Equation 3]

Inhibition Rate (%) = ((No Absorption Absorption-No Absorption Absorption) / No Absorption Absorption) * 100)

TABLE 4

Tomato extract (% by weight) Melanin Reduction (%) 0.001 27.9 0.01 32.06 0.1 18.48 One 10.92 10 8.42

[Test Example 4]

The test which measured the whitening effect of the cream of manufacture example 1, manufacture example 2, and comparative manufacture example 1-comparative manufacture example 5 which were manufactured by the Example was done. 35 healthy males and females were placed in the lower arms of both arms with 2 * 2㎠ areas, and aluminum foil was wrapped around the arms to irradiate UV light only at the test site. 10-7 erg / cm <3> / circuit was investigated 3 times consecutively once a day. Before the irradiation, the test site was washed well with 70% aqueous alcohol solution, and after irradiation, the cream of the above examples and comparative preparations containing tomato extract was applied twice a day for 8 weeks. After 8 weeks of application, the degree of pigmentation was visually determined and evaluated in three stages of good effect, normal effect and no effect depending on how much pigmentation was suppressed. The results are shown in Table 5.

TABLE 5

Effective usually no effect Preparation Example 1 20 12 3 Preparation Example 2 24 10 One Comparative Production Example 1 One One 33 Comparative Production Example 2 13 15 7 Comparative Production Example 3 15 15 5 Comparative Production Example 4 27 8 0 Comparative Production Example 5 29 5 One

As can be seen in Table 5, in the case of cosmetics containing more than 10.0% by weight tomato extract was able to see more than 90% whitening effect. However, as will be described later, Comparative Preparation Example 4 and Comparative Preparation Example 5 exceeding the weight range of the tomato extract according to the present invention is not preferable because it causes discoloration or separation symptoms to make the cosmetic unstable.

[Test Example 5]

Test Example 5 measured the antioxidant power of tomato extract and the degree of automatic oxidation inhibition and DPPH free radical inhibition by Funita et al. Free Radical Scavenging. Antioxidant effect was obtained by adding 0.1 mg of 2 mg / ml linolenic acid, 10 mg / ml Tween20, 0.2 M phosphate buffer (pH 7.4), and sample to make 0.1 ml, and then incubating for 24 hours at 37 degrees Celsius, followed by 80% ethanol. 0.1 ml, 30% ammonium thiocyanate 0.1 ml, 20 mM ferrous ammonium sulfate-0.1 ml of 3.5% hydrochloric acid were added, and after 3 minutes, the absorption wavelength was measured at 500 nm, and the antioxidant effect was compared with the control group without the antioxidant effect sample. The effect was measured in%. The free radical scavenging effect was obtained by comparing the absorbance at 516 nm after 30 minutes at 37 degrees Celsius with 1.0 ml of 0.1 mM DPPH metanolic acid and 1.0 ml of ethanol. The results are shown in Table 6.

TABLE 6

matter Antioxidative effect(%) Free radical scavenging activity (%) Arbutin 1.0% Ethanol Solution N.D ** N.D ** Ginkgo leaf extract 1.0% ethanol solution 68.0 69.5 Tomato Extract 1.0% Ethanol Solution 81.5 81.0 **: no measurement

As can be seen from Table 6, the tomato extract was confirmed that both the antioxidant and DPPH free radical inhibition effect on linolenic acid is excellent.

[Test Example 6]

The stability tests of the cream formulations of Preparation Example 1, Preparation Example 2, and Comparative Preparation Examples 1 to 5 were prepared in Examples. Samples stored in an opaque glass jar in a thermostat kept at a constant 45 degrees Celsius, respectively, for 12 weeks, and the opaque glass jar in a completely shaded refrigerator kept at 4 degrees Celsius. The separation and discoloration of the samples stored for 12 weeks and the samples stored for 12 weeks in a circulation chamber circulating at 37 degrees to -5 degrees Celsius were measured. The results of the evaluation of the product separation and the degree of discoloration classified into the following six grades are shown in Table 7 below.

Product discoloration evaluation criteria:

0: no change 1: very little separation (discoloration)

2: Separate slightly (discolored) 3: Separate slightly (discolored)

4: severely separated (discolored) 5: extremely severely separated (discolored)

TABLE 7

Separation and discoloration degree of test substance Preparation Example 1 Preparation Example 2 Comparative Preparation Example 1 Comparative Production Example 2 Comparative Production Example 3 Comparative Production Example 4 Comparative Production Example 5 45 degrees celsius 0 0 0 0 0 2 3 4 degrees Celsius 0 0 0 0 0 0 One Cycling 0 0 0 0 0 3 3

As can be seen in Table 7, tomato extract-containing cosmetics were found to be stable at 4 degrees Celsius, 45 degrees Celsius, without discoloration or separation symptoms in a circulating test when the tomato extract contains 0.0 to 30.0 wt%. In addition, at 30.0 wt% or more, there was discoloration and separation symptoms, indicating that the stability was poor. In addition, as confirmed through the above Experimental Example 1 and Experimental Example 4, when less than 10% by weight of the tomato extract according to the present invention can be seen that the skin moisturizing effect is reduced and there is almost no whitening effect. Therefore, it can be seen that the cosmetic composition containing the tomato extract according to the present invention preferably contains 10.0 to 30.0 wt% of the tomato extract extracted from the mixed solvent of 1,3 butylene glycol and olive oil based on the total weight of the composition. have.

On the other hand, in the present specification, the present inventors described the test example with only the example of the cream formulation among various formulations performed according to the embodiment according to the present invention, but formulations other than cream, that is, skin lotion, cream, pack, gel, lotion, etc. It was confirmed that the same result was obtained for all formulations such as the type and the transdermal dosage form. Therefore, the technical spirit of the present invention is not limited or limited to the formulation of the cream, and can be variously modified and implemented by those skilled in the art.

As described above, according to the cosmetic composition containing the tomato extract according to the present invention, it is excellent in moisturizing power, antioxidant power and whitening effect, and also can obtain a very stable effect without discoloration and separation symptoms. Thereby, the cosmetic composition containing the tomato extract according to the present invention may have an excellent effect on the improvement of the skin and the like.

Claims (2)

delete In the cosmetic composition, a mixed solvent of 1,3 butylene glycol and olive oil, the tomato extract extracted from a mixed solvent of about 1: 3 butylene glycol and olive oil in a weight ratio of about 1: 1 to the total weight of the composition 10.0 to 30.0% by weight relative to the cosmetic composition comprising a tomato extract.