KR102178510B1 - Functional cosmetic composition comprising complex extract of gold kiwi peel and pomegranate peel as effective ingredient, and manufacturing method thereof - Google Patents

Abstract

A functional cosmetic composition containing a complex extract of gold kiwi peels and pomegranate peels as an active component of the present invention has an excellent effect of inhibiting inflammation, and further has anti-wrinkle, skin moisturizing, sebum reduction, dead skin cell reduction, and antioxidant effects. An object of the present invention is to provide a cosmetic composition which has no cytotoxicity and less skin irritation.

Description

Functional cosmetic composition containing complex extracts of gold kiwi skin and pomegranate skin as active ingredients, and its manufacturing method {FUNCTIONAL COSMETIC COMPOSITION COMPRISING COMPLEX EXTRACT OF GOLD KIWI PEEL AND POMEGRANATE PEEL AS EFFECTIVE INGREDIENT, AND MANUFACTURING METHOD THEREOF}

The present invention relates to a functional cosmetic composition showing anti-inflammatory effects, comprising a complex extract of gold kiwi skin and pomegranate skin.

NO is known to have various 　 actions such as blood pressure regulation, platelet aggregation, and signal transmission in vivo. In particular, pro-inflammatory cytokine and LPS act on immune cells and increase NO production by the action of inducible nitric oxide synthase (iNOS) from L-arginine. Excessive NO production is related to chronic inflammation, and many studies have been conducted on the development of selective NO biosynthesis inhibitors to control chronic inflammation through the regulation of iNOS activity (Sharma et al., 2007; Korhonen et al., 2005).

On the other hand, the aging of skin cells that are closely related to the skin wrinkles can be classified into natural aging caused by aging and aging caused by external environment. Natural aging is difficult to control because genetic factors are involved, and studies to suppress aging by controlling environmental factors such as ultraviolet rays, oxidation, and drying have been conducted.

The environmental factors mainly destroy or denature collagen protein, elastin protein, etc. contained in the dermal layer, causing wrinkles. In particular, ultraviolet rays oxidize skin cells (fibroblasts) and cause reduction and destruction of elastin production.When elastin-degrading enzymes are expressed in the skin by ultraviolet rays, etc., this enzyme decomposes normal elastin in the skin and reduces skin elasticity. Is brought. In addition, free radicals mass-produced by the above factors may cause protein destruction in skin cells, oxidation of sugars, genetic modification, and the like by oxidation, thereby causing aging and wrinkles in the skin.

As the use of cosmetics containing a large amount of chemical ingredients has caused various side effects and problems due to skin damage, there have been attempts to manufacture cosmetics by adding extracts extracted from various plants, fruits, and herbal medicines. In particular, there has been a need to search for a material that can be safely used for the human body while having functions such as anti-oxidation and anti-wrinkle.

Fruits may be candidates for meeting these demands due to their low cytotoxicity while containing several substances useful for physiological activity. However, in order to extract the desired active ingredient from fruit in a high content, a huge amount of raw materials are required, so there is a problem of product cost increase, and it is necessary to consider the problem of environmental pollution due to consumption of food resources and generation of by-products. have.

On the other hand, after processing vegetable materials such as fruits, most of the peels generated as by-products are being disposed of as garbage, and measures for environmentally friendly disposal are also needed.

Accordingly, the need for a cosmetic raw material that can secure a desired active ingredient in a high content, is not resource-consuming, and can not excessively increase the cost or intensify environmental pollution increases.

An object of the present invention is to provide a cosmetic composition exhibiting anti-inflammatory effect by inhibiting NO production.

Another object of the present invention is to provide a cosmetic composition exhibiting the efficacy of preventing or improving wrinkles by inhibiting elastase.

Another object of the present invention is to provide a cosmetic composition that has no cytotoxicity and less skin irritation.

Another object of the present invention is to provide a cosmetic composition with an increased content of natural phenolic compounds.

According to an aspect of the present invention, a cosmetic composition containing a complex extract of gold kiwi skin and pomegranate skin as an active ingredient and exhibiting anti-inflammatory effect can be provided.

The cosmetic composition may further have anti-wrinkle effect, skin moisturizing, sebum reduction, dead skin cell reduction or antioxidant effect.

Specifically, the blending ratio of the gold kiwi skin extract and the pomegranate skin extract among the complex extracts may be 1: 0.5 to 1: 2.

The composite extract may be contained in an amount of 0.001% to 60% by weight based on the total weight of the cosmetic composition.

The cosmetic composition is not limited thereto, but may be a formulation of a lotion, serum, essence, lotion, cream, powder, foundation, pack, patch, mask sheet, gel ointment, spray or detergent.

In addition, according to another aspect of the present invention, it is possible to propose a method for preparing a cosmetic composition having an anti-inflammatory effect, containing the complex extract of the aforementioned gold kiwi peel and pomegranate peel as an active ingredient.

The method includes the step of preparing a complex extract by mixing the extract of gold kiwi skin and the extract of pomegranate skin, or mixing and then extracting the dried gold kiwi skin pulverized product and the dry pomegranate peel pulverized product. In this case, the blending ratio of the gold kiwi skin extract and the pomegranate skin extract, or the blending ratio of the dry gold kiwi crushed product and the dried pomegranate peel crushed product may be 1: 0.5 to 1: 2, and specifically 1:1.

The method for preparing the cosmetic composition is to add 1 to 100 times the weight of a solvent to the dried raw material pulverized product of gold kiwi skin and pomegranate skin, followed by extraction with a water bath for 30 minutes to 360 hours at a temperature range of 10 to 120°C, cooling with reflux It may include performing one or more of extraction, ultrasonic extraction, cold needle extraction, and percolation.

In addition, the manufacturing method of the cosmetic composition is added to a general cosmetic preparation composition in an amount of 0.001% to 60% by weight of the complex extract, and then lotion, serum, essence, lotion, cream, powder, foundation, pack, according to a conventional method, It may include the step of formulating a formulation such as a patch, a mask sheet, a gel ointment, a spray or a detergent.

The cosmetic composition containing the complex extract of gold kiwi peel and pomegranate peel of the present invention as an active ingredient has excellent anti-inflammatory effect, and further retains the functions of wrinkle improvement, skin moisturizing, sebum reduction, dead skin cell reduction, and antioxidant effect.

1 is a graph showing the cytotoxicity test results for Preparation Example and Comparative Preparation Example of the present invention.
2 is a graph showing the results of anti-inflammatory tests for Preparation Example and Comparative Preparation Example of the present invention.
3 is a graph showing the results of evaluating the elastase inhibitory activity for Preparation Examples and Comparative Preparation Examples of the present invention.
Figure 4 is a graph showing the antioxidant activity evaluation results for Preparation Example and Comparative Preparation Example of the present invention.
5 is a graph showing the results of skin improvement evaluation of the moisturizing degree retention rate, the sebum amount reduction rate, and the angular mass reduction rate for Examples and Comparative Examples of the present invention.
6 is a graph showing the sensory evaluation results of feeling of use and efficacy for Examples and Comparative Examples of the present invention.

The present invention relates to a functional cosmetic composition comprising a complex extract of gold kiwi skin and pomegranate skin, which exhibits anti-inflammatory effects through the NO production inhibitory activity.

The complex extract further has anti-wrinkle effect and antioxidant ability, and can realize the effect of improving and maintaining skin moisture, reducing sebum amount, and reducing angular mass of a cosmetic composition including the same by increasing the content of natural phenolic compounds. .

The gold kiwi is one of the various species of kiwi fruit, a deciduous vine plant of genus Actinidia, which has been modified and bred by the natural method of grafting new kiwi seeds to green kiwi trees.

In addition, the flesh and skin of gold kiwi contain vitamins C and E, beta-carotene of carotenoids, folic acid, potassium, calcium, phosphorus, etc., so as to strengthen immunity and relieve fatigue, arthritis, osteoporosis, osteomalacia, arteriosclerosis, cataract , It is known to be effective in preventing adult diseases, etc.

On the other hand, pomegranate (Punicagranatum L.) is a plant native to Southwest Asia, Northwest India and California, USA, and is now widely spread in subtropical and tropical regions. Since ancient times, pomegranate, particularly red pomegranate, has been known as a tonic, and is known to have a good effect in preventing hypertension and arteriosclerosis. In addition, it contains a large amount of 38 to 47% water-soluble sugars, and contains various vitamins and minerals.

In the present invention, the gold kiwi peel extract or pomegranate peel extract is not particularly limited, but may be specifically used in various forms such as an extract, a powder obtained by drying the extract, and a concentrate obtained by concentrating and filtering the extract.

The extract may be, for example, in the form of a tink, concentrate, extract, flow extract, etc. containing alcohol, purified water, oil, and the like as a leaching solvent.

The detailed extraction method of the gold kiwi or pomegranate peel extract will be described in more detail later in the specification of the present application.

Specifically, the blending ratio of the gold kiwi skin extract and the pomegranate skin extract among the complex extracts may be 1: 0.5 to 1: 2.

Within the above blending ratio range, improved antioxidant effect, improved skin moisturization and maintenance, sebum amount reduction, angular mass reduction, and phenol content, along with improved NO generation inhibition and elastase inhibitory activity may be achieved.

In one embodiment, the blending ratio of the gold kiwi skin extract and the pomegranate skin extract in the composite extract may be measured based on the solid weight of the extract. In this case, the weight of the solid material of the extract may be a solid product obtained by freeze-drying after extraction by adding a solvent to the dried gold kiwi skin crushed product and the dried pomegranate peel crushed product or a mixture thereof.

In another embodiment, the blending ratio of the gold kiwi skin extract and the pomegranate skin extract in the composite extract may be measured based on the solid weight of the raw material. In this case, the weight of the solid material may be a solid product of a dried gold kiwi skin pulverized product and a dry pomegranate peel pulverized product, or a mixture thereof before the extraction solvent is added.

The composite extract may be contained in an amount of 0.001% to 60% by weight, specifically 1% to 55% by weight, and more specifically 20% to 55% by weight, based on the total weight of the cosmetic composition. Functionality expected within the above range can be expressed.

The cosmetic composition may be a formulation of any one of lotion, serum, essence, lotion, cream, powder, foundation, pack, patch, mask sheet, gel ointment, spray, and detergent, but is not limited thereto.

The cosmetic composition of the present invention may further include a composition for preparing a cosmetic together with the complex extract.

The composition for preparing a cosmetic is for implementing a cosmetic effect according to the intended use and formulation of the cosmetic by acting together with the complex extract, and is not particularly limited as long as it is a component used for general cosmetic subjects, excipients, and the like.

Specifically, as the composition for preparing the cosmetic composition, solvents, fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, plant oils , Pigments, fragrances, blood circulation accelerators, cooling agents, sweat secretion inhibitors, etc. can be mixed and used.

For example, the cosmetic preparation composition is purified water (water), alcohol (alcohol), glycerin (glycerin), myristic acid (myristic acid), butylene glycol (butylene glycol), potassium hydroxide (potassium hydroxide) , Polyethylene glycol, stearate (sterate), stearic acid, behenyl alcohol, microcrystalline wax, phenoxyethanol, ethylhexyl glycerin ( ethylhexylglycerin), tocopheryl acetate, 1,2-hexanediol, caprylyl glycol, hydroxyethylene cellulose, hydroxyethyl cellulose ethyl cellulose), xanthan gum, collagen, sodium citrate, citric acid, magnesium ascorbate, allantoin, panthenol, betaine ( betaine), Cetearyl Alcohol, Sunflower Oil, Cetearyl Olivate, Sorbitan Olivate, Glyceryl Stearate and Fragrance ) May contain one or more of.

Another aspect of the present invention relates to a method for preparing a functional cosmetic composition containing the above-described complex extract of the skin of gold kiwi and pomegranate skin as an active ingredient. The method includes preparing a complex extract by mixing the extract of gold kiwi skin and the extract of pomegranate skin, or mixing and then extracting the dried gold kiwi crushed product and the dried pomegranate peel crushed product.

In the manufacturing method of the cosmetic composition of the present invention, the process of preparing the extract of the gold kiwi peel, the extract of the pomegranate peel, or the complex extract is not particularly limited, and can be obtained through various methods known in the field to which the present invention belongs.

For example, the process of preparing the extract of the gold kiwi peel, the extract of the pomegranate peel, or the complex extract is a temperature of 10 to 120°C after adding a solvent having a weight of 1 to 100 times to the pulverized dry raw material for each extraction object. For 30 minutes to 360 hours in the range, it may include performing one or more of the bath extraction, reflux cooling extraction, ultrasonic extraction, cold needle extraction, and percolation.

Each of the extraction targets refers to a mixture of gold kiwi skin and pomegranate skin in the case of a gold kiwi skin extract, a pomegranate skin in the case of a pomegranate skin extract, and a complex extract.

The solvent is not particularly limited as long as it can be used as an extraction solvent for a cosmetic composition, but may specifically be water, an organic solvent, or a mixed solvent thereof.

The water may be alkaline water, and the organic solvent may be an anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms such as ethanol, propanol, methanol, and n-butanol; Polar organic solvents such as acetone and 1,3-butylene glycol; Non-polar organic solvents such as ether, hexane, benzene, chloroform, and ethyl acetate; Vegetable organic solvents such as soybean oil and sesame oil; And the like. The organic solvent may be used alone or in combination of two or more. In this case, when a mixed solvent is used, the mixed composition and ratio may be appropriately mixed according to the purpose.

In one embodiment, one or more lower alcohols of ethanol, methanol, n-butanol, and propanol as a solvent used in the extraction; One or more polar organic solvents of 1,3-butylene glycol and acetone; And a mixed solvent containing alkaline water. In this case, when the extract of the gold kiwi skin, the extract of the pomegranate skin, or the complex extract is prepared, the extraction efficiency of the active ingredient may be improved.

In another embodiment, one or more lower alcohols of ethanol, methanol, n-butanol, and propanol as a solvent used in the extraction; And a mixed solvent containing water can be used. In this case, the extraction balance for both the skin of the gold kiwi and the skin of pomegranate is excellent, so that the extraction efficiency of the active ingredient can be improved when preparing the complex extract.

The amount of the solvent added during the extraction is not particularly limited, but may be 1 to 100 times the weight, specifically 2 to 80 times, more specifically 5 to 50 times, to the pulverized dry raw material for each extraction target. Within the above range, it is possible to increase the time required for extraction and the content of the active ingredient to be extracted more efficiently.

The temperature during the extraction is not particularly limited, but may be performed in a temperature range of 10 to 120°C, for example, 50 to 120°C or 10 to 40°C. Within the above range, it is possible to increase the time required for extraction and the content of the active ingredient to be extracted more efficiently.

The extraction time is not particularly limited, but may be performed for 30 minutes to 360 hours, for example, 30 minutes to 72 hours, 30 minutes to 24 hours, or 168 hours to 360 hours. Within the above range, it is possible to increase the time required for extraction and the content of the active ingredient to be extracted more efficiently.

The extraction method is not particularly limited, but may include performing any one or more of a bath extraction, reflux cooling extraction, ultrasonic extraction, cold needle extraction, and percolation. Within the above range, it is possible to increase the time required for extraction and the content of the active ingredient to be extracted more efficiently.

The number of extractions during the extraction is not particularly limited, but may be performed once or more for each extract, and specifically, may be repeated once to five times. Within the above range, it is possible to increase the time required for extraction and the content of the active ingredient to be extracted more efficiently.

In one embodiment, the extraction may be performed by heating and refluxing cooling at 50 to 120° C. for 30 minutes to 24 hours after each extraction target and extraction solvent are added to an extractor equipped with a reflux cooler. In this case, the heating-reflux cooling extraction may be a method of refluxing while heating each target material to which a solvent is added, and then cooling to extract. In this case, it is possible to further improve the extraction efficiency while minimizing the amount of solvent loss due to heating.

In another embodiment, the extraction may be performed by adding an extraction solvent to each extraction target and then immersing at 10 to 40° C. for 1 to 15 days to perform cold sedimentation. In this case, manufacturing cost can be reduced by omitting equipment required for heating and reducing energy consumption.

In another embodiment, the extraction may be performed at room temperature for 1 to 200 hours after the extraction solvent is added to each extraction target. In this case, the content of the active ingredient can be further increased.

In another embodiment, the extraction may be extracted by adding an extraction solvent to each extraction target and then bathing at 50 to 120° C. for 30 minutes to 72 hours. In this case, the content of the active ingredient can be increased more efficiently.

In addition, each of the extracts may include not only an extraction material extracted by the above-described extraction solvent, but also an extraction material extracted through a conventional purification process. For example, active fractions obtained through various purification methods additionally performed, such as separation using an ultrafiltration membrane having a certain molecular weight cut-off value, may also be included in the extract of the present invention.

To prepare the complex extract in the cosmetic composition manufacturing method of one embodiment, after each of the gold kiwi peel extract and the pomegranate peel extract are prepared, they may be mixed in a weight ratio of 1: 0.5 to 1: 2.

To prepare the complex extract in the cosmetic composition manufacturing method of another embodiment may be extracted by adding an extraction solvent after mixing the dried gold kiwi crushed product and the dried pomegranate peel crushed product in a weight ratio of 1: 0.5 to 1: 2 .

The manufacturing method of the cosmetic composition is after adding 0.001% to 60% by weight of the complex extract to a general cosmetic preparation composition, and then lotion, serum, essence, lotion, cream, powder, foundation, pack, patch, mask sheet, gel It may further include formulating in any one of ointment, spray and detergent. The composition for preparing a cosmetic is for realizing a cosmetic effect according to the intended use and formulation of the cosmetic by acting together with the complex extract, and is not particularly limited as long as it is a component used for general cosmetic subjects and excipients, and specific examples are described above. Follow the same content as the description.

In addition, the formulation method is not particularly limited, and may be obtained through various methods known in the field to which the present invention belongs.

Hereinafter, the configuration and operation of the present invention will be described in more detail through preferred embodiments of the present invention. However, this has been presented as a preferred example of the present invention, and for any reason cannot be construed as limiting the present invention.

Contents not described herein can be sufficiently technically inferred by those skilled in the technical field to which the present invention pertains, and the description thereof will be omitted.

Preparation Example 1: Preparation of complex extract (weight ratio 1:1) of gold kiwi skin and pomegranate skin

After the peel was separated from the gold kiwi fruit, it was completely dried and pulverized. After the peel was separated from the pomegranate fruit, it was completely dried and pulverized.

After mixing 15 g of the dried gold kiwi skin pulverized product and 15 g of the dried pomegranate skin pulverized product obtained as described above, 20 times 70% ethanol was added to the total weight of the mixture, and the composite extract was heated at 80° C. for 2 hours. Was prepared. After repeating the above process once more, the filtrate obtained by filtration through a 0.45 μm filter was concentrated under reduced pressure and freeze-dried.

Preparation Example 2: Preparation of complex extract (weight ratio 2:1) of gold kiwi skin and pomegranate skin

After the peel was separated from the gold kiwi fruit, it was completely dried and pulverized. After the peel was separated from the pomegranate fruit, it was completely dried and pulverized.

After mixing 20 g of the dried gold kiwi skin pulverized product and 10 g of the dried pomegranate skin pulverized product obtained as described above, 20 times of 70% ethanol was added to the total weight of the mixture, and the composite extract by heating extraction method at 80° C. for 2 hours Was prepared. After repeating the above process once more, the filtrate obtained by filtration through a 0.45 μm filter was concentrated under reduced pressure and freeze-dried.

Preparation Example 3: Preparation of complex extract (weight ratio 1:2) of gold kiwi skin and pomegranate skin

After the peel was separated from the gold kiwi fruit, it was completely dried and pulverized. After the peel was separated from the pomegranate fruit, it was completely dried and pulverized.

After mixing 10 g of the dried gold kiwi skin pulverized product and 20 g of the dried pomegranate skin pulverized product obtained as described above, 20 times 70% ethanol was added to the total weight of the mixture, and the composite extract was heated at 80° C. for 2 hours. Was prepared. After repeating the above process once more, the filtrate obtained by filtration through a 0.45 μm filter was concentrated under reduced pressure and freeze-dried.

Comparative Preparation Example 1: Preparation of Gold Kiwi Skin Extract

After the peel was separated from the gold kiwi fruit, it was completely dried and pulverized. To 30 g of the dried gold kiwi skin pulverized product obtained as described above, 20 times 70% ethanol was added to the total weight, and a composite extract was prepared by heating extraction at 80°C for 2 hours. After repeating the above process once more, the filtrate obtained by filtration through a 0.45 μm filter was concentrated under reduced pressure and freeze-dried.

Comparative Preparation Example 2: Preparation of pomegranate peel extract

After the peel was separated from the pomegranate fruit, it was completely dried and pulverized. To 30 g of the dried pomegranate skin pulverized product obtained as described above, 20 times 70% ethanol was added to the total weight, and a composite extract was prepared by heating extraction at 80° C. for 2 hours. After repeating the above process once more, the filtrate obtained by filtration through a 0.45 μm filter was concentrated under reduced pressure and freeze-dried.

Comparative Preparation Example 3: Preparation of green kiwi peel extract

After the peel was separated from the green kiwi fruit, it was completely dried and pulverized. To 30 g of the dried green kiwi skin pulverized product obtained as described above, 20 times 70% ethanol was added to the total weight, and a composite extract was prepared by heating extraction at 80° C. for 2 hours. After repeating the above process once more, the filtrate obtained by filtration through a 0.45 μm filter was concentrated under reduced pressure and freeze-dried.

Example 1: Preparation of lotion formulation containing complex extract

30% by weight of the complex extract prepared in Preparation Example 1, 62.65% by weight of purified water, 5% by weight of butylene glycol, 1.5% by weight of 1,2-hexanediol, 0.5% by weight of allantoin, 0.05% by weight of panthenol, and 0.3% by weight of betaine % Was added to the stirrer, and then mixed to prepare a cosmetic composition of a lotion formulation.

Example 2: Preparation of Serum Formulation Containing Complex Extract

50% by weight of the complex extract prepared in Preparation Example 1, 39.7% by weight of purified water, 7% by weight of butylene glycol, 1.5% by weight of 1,2-hexanediol, 1% by weight of glycerin, 0.5% by weight of betaine, and 0.3 of xanthan gum After adding the weight% to the stirrer, it was mixed to prepare a cosmetic composition in a serum formulation.

Example 3: Preparation of cream formulation containing complex extract

55% by weight of the composite extract prepared in Preparation Example 1, 26.6% by weight of purified water, 3% by weight of glycerin, 6% by weight of butylene glycol, 0.5% by weight of betaine, 1.5% by weight of hydroxyethyl cellulose, 0.8% by weight of cetearyl alcohol %, sunflower oil 1.8 wt%, cetearyl olivate 0.75 wt%, sorbitan olivate 0.75 wt%, glyceryl stearate 2.5 wt%, ethyl hexyl glycerin 0.5 wt%, and xanthan gum 0.3 wt% were added to a stirrer Then, it was mixed to prepare a creamy cosmetic composition.

Comparative Example 1

A cosmetic composition of a lotion formulation was prepared in the same manner as in Example 1, except that the complex extract prepared in Preparation Example 1 was replaced with the same amount of purified water.

Comparative Example 2

A cosmetic composition in a serum formulation was prepared in the same manner as in Example 2, except that the complex extract prepared in Preparation Example 1 was replaced with the same amount of purified water.

Comparative Example 3

A cream-formed cosmetic composition was prepared in the same manner as in Example 3, except that the composite extract prepared in Preparation Example 1 was replaced with the same amount of purified water.

Test Example 1: Toxicity evaluation test

DMSO was added to the extract samples according to Preparation Example and Comparative Preparation Example, diluted to the concentration shown in Table 1 below, and the degree of cytotoxicity according to the concentration was evaluated.

A mouse macrophage cell line RAW 264.7 10% FBS (fetal bovine serum) and 1% penicillin / streptomycin DMEM medium containing hygromycin is included (Dulbecco's Modified Eagle Medium) by the same coefficient by 1Х10 ⁴ cells / ㎖ a 96-well plate using a frequency divider Then, it was incubated for 24 hours at 37°C and 5% carbon dioxide.

Samples according to Preparation Example and Comparative Preparation Example were mixed with a medium by concentration to the cultured cells, and then 1 mL was added to each well and cultured for 24 hours at 37°C and 5% carbon dioxide. After incubation for 24 hours, only the supernatant of each well was taken separately, and then WST-1 assay solution (ez-cytox) was added to each well, reacted for 2 hours in an incubator, and absorbance was measured at 450 nm with an ELISA reader. Cell viability was calculated by and the results are shown in Table 1 below.

[Calculation 1]

Cell viability (%) = [(absorbance of sample treated group / absorbance of untreated group) Х 100]

Concentration (㎍/mL) Manufacturing Example 1 Manufacturing Example 2 Manufacturing Example 3 Comparative Production Example 1 Comparative Production Example 2 Comparative Production Example 3 0 100.00 100.00
100.00
100.00 100.00
100.00
3.125 104.83 103.19 101.96 104.47 104.84 101.71 6.25 104.47 103.82 104.15 104.03 103.98 97.91 12.5 104.86 103.84 103.62 103.66 104.23 99.80 25 105.46 103.59 101.84 102.88 103.18 100.81 50 103.54 103.15 102.36 102.33 102.73 100.27 100 104.69 103.47 103.21 103.66 102.72 98.54

As can be seen in Table 1 and FIG. 1, Preparation Examples 1 to 3 of the composite extract of the present invention confirmed that the cell viability exceeded 100 at all the tested concentrations, so that it can be safely used.

Test Example 2: Evaluation of anti-inflammatory activity

DMSO was added to the extract samples according to Preparation Example and Comparative Preparation Example, diluted to the concentration shown in Table 1 below, and the degree of cytotoxicity according to the concentration was evaluated.

A mouse macrophage cell line RAW 264.7 10% FBS (fetal bovine serum) and 1% penicillin / streptomycin DMEM medium containing hygromycin is included (Dulbecco's Modified Eagle Medium) by the same coefficient by 1Υ10 ⁴ cells / ㎖ a 96-well plate using a frequency divider Then, it was incubated for 24 hours at 37°C and 5% carbon dioxide.

Samples according to Preparation Example and Comparative Preparation Example were mixed with a medium by concentration to the cultured cells, and then 1 mL was added to each well and cultured for 24 hours at 37°C and 5% carbon dioxide.

At this time, LPS (Lipopolysaccharide), which is an inflammation inducing factor expressing NO, was treated at a concentration of 1 ug/ml and incubated for 24 hours at 37° C. and 5% carbon dioxide. After taking only the culture solution of each well separately, 100 μl of each culture solution is taken into a 96-well plate and 50 μl of Griess reagent A (N-1-Naphthylethylenediamine (NEDHC)) and 50 μl of Griess reagent B (Sulfanilamide) using a NO assay kit Each was put into each reaction for 10 minutes, and then the absorbance was measured at 540 nm using an ELISA plate reader. Table 2 shows the results as a percentage based on 1mM nitrite standard solution.

Concentration (㎍/mL) Manufacturing Example 1 Manufacturing Example 2 Manufacturing Example 3 Comparative Production Example 1 Comparative Production Example 2 Comparative Production Example 3 0 0.00
0.00
0.00
0.00
0.00
0.00
3.125 0.94 0.29
0.14
8.64
0.16
6.62
6.25 2.64 0.43 0.57 11.23 0.31 6.91 12.5 3.39
1.00
2.00
12.10
1.73
9.79
25 3.77 3.28
7.00
13.25
9.60
12.38
50 16.59 10.85
15.57
13.82
20.15
12.10
100 36.20 23.56 36.28 19.01 32.90 13.25

As a result, as can be seen in Table 2 and Figure 2, compared to the case of using the complex extract of the present invention gold kiwi peel extract or pomegranate peel extract alone, the two extracts were mixed at a weight ratio of 1:1 or 1:2. When the synergistic effect of suppressing NO production appears large, this means that the composition can exhibit excellent anti-inflammatory activity.

Test Example 3: Wrinkle prevention or improvement evaluation

DMSO was added to each sample of the extract according to Preparation Example and Comparative Preparation Example, diluted to the concentration shown in Table 3 below, and the elastase inhibitory activity according to the concentration was evaluated.

Samples according to Preparation Examples and Comparative Preparation Examples were dissolved in 10 mM Tris-HCl buffer (pH 8.0) at a concentration of 1.6 mM N-succinyl-(Ala)3-p-nitroanilide substrate and elastase derived from porcine pancreas type IV 0.4 U/ml was mixed and reacted at 22° C. for 5 minutes, and then placed in a 96 well plate and absorbance was measured at 410 nm.

At this time, a sample without a sample was used as a control, and the result was calculated using the following calculation formula 2, and the results are shown in Table 3 below. At this time, oleanolic acid was used as a positive control, and the concentration of oleanolic acid was 9.37 μg/ml when the elastinase inhibitory activity was 50% .

[Calculation 2]

Elastase inhibitory activity (%) = 100-[(absorbance of group treated with sample / absorbance of group not treated with sample) Х 100]

Concentration (㎍/mL) Manufacturing Example 1 Manufacturing Example 2 Manufacturing Example 3 Comparative Production Example 1 Comparative Production Example 2 Comparative Production Example 3 0 0.00 0.00
0.00
0.00
0.00
0.00
1.5625 2.05 5.69
6.14
4.12
6.58
5.28
3.125 3.42 8.25
6.55
3.18
9.28
4.60
6.25 6.11 9.00
9.14
2.96
13.63
9.45
12.5 9.00 11.09
10.78
6.72
12.46
11.32
25 15.36 15.36
22.51
9.69
15.04
13.70
50 31.12 20.19
23.47
14.32
12.93
16.17
100 37.57 27.49 30.01 19.74 25.26 17.19

As can be seen in Table 3 and Figure 3, the composite extract of the present invention is compared to the case of using gold kiwi skin extract or pomegranate peel extract alone, when the two extracts are mixed at a weight ratio of 1:1 or 1:2 There is a remarkable synergistic effect in the anti-wrinkle activity, which means that the composition has an excellent anti-wrinkle effect.

Test Example 4: Evaluation of antioxidant efficacy

In order to examine the antioxidant effect, DMSO was added to the extracts according to Preparation Examples and Comparative Preparation Examples, diluted to the concentration shown in Table 4 below, and the degree of DPPH inhibition according to the concentration was evaluated.

50 µl of samples according to Preparation Example and Comparative Preparation Example were mixed with 450 µl of 0.1M DPPH (1,1-diphenyl-2-picrylhydrazyl) solution at different concentrations and reacted at 4° C. for 30 minutes. Upon completion of the reaction, each sample was used as a control, and the free radical scavenging activity (%) of the sample was calculated by the following calculation formula 3, and the results are shown in Table 4 below. At this time, ascorvic acid was used as a positive control. When the free radical scavenging activity was 50%, the concentration of ascorbic acid was 2.09㎍/㎖.

[Calculation 3]

Free radical scavenging ability (%) = [(absorbance of sample treated group / absorbance of untreated group) Х 100]

In addition, the minimum concentration (SC50) required to remove 50% of the radicals generated for each sample is expressed in the unit of μg/ml and is shown in Table 4.

Concentration (㎍/mL) Manufacturing Example 1 Manufacturing Example 2 Manufacturing Example 3 Comparative Preparation Example 1 Comparative Preparation Example 2 Comparative Preparation Example 3 0 0.00
0.00 0.00 0.00 0.00 0.00 1.5625 6.15 5.67
7.46
2.34
12.08 0.00
3.125 11.92 11.53
15.11
2.68
25.00 2.52
6.25 22.84 25.71
31.55
8.36
44.58 3.77
12.5 43.41 50.47
61.95
16.05
70.56 7.86
25 67.75 82.23
81.45
30.43
74.72 17.30
50 73.90 85.63
85.09
50.5
78.75 29.56
100 78.80 89.04
88.53
61.87
82.08 53.14
SC50 14.29 12.36 10.07 69.68 8.2 91.24

As a result, as shown in Table 4 and FIG. 4, it can be seen that the antioxidant activity is generally higher when the two extracts are mixed compared to the case of using the gold kiwi peel extract or the pomegranate peel extract alone. In particular, when the test concentration was used at 25 ug/ml or more, the antioxidant activity of the composite extract obtained by mixing the two extracts at 2:1 and 1:2 was further increased.

Test Example 5: Measurement of the content of phenolic compounds

It is known that plant-derived natural phenolic compounds act on the skin, promoting cell regeneration by physiological activity of skin cells, improving antioxidant activity, reducing sebum mass, and improving skin by reducing angular mass.

DMSO was added to each sample of the extract according to Preparation Example and Comparative Preparation Example, and the contents of phenol and flavonoids belonging to the phenolic compound were measured, respectively.

The total phenol content was stabilized at room temperature for 5 minutes by adding 100 µl of Folin-Ciocalteu reagent to 100 µl of the sample, and then 100 µl of 10% sodium carbonate was added and reacted at 30° C. for 1 hour, and the absorbance was measured at 760 nm. The total phenol content of each sample was calculated from the standard calibration curve obtained by preparing gallic acid as a standard by concentration.

To measure the total flavonoid content, 1 ml of diethylene glycol was added to 100 μl of the sample, mixed well, 100 μl of 1N soduum hydroxide was added, reacted at 37° C. for 1 hour, and absorbance was measured at 420 nm. The total phenol content of each sample was calculated from the standard calibration curve obtained by preparing naringin as a standard by concentration.

Table 5 shows the content of phenol compared to each reference compound.

density Manufacturing Example 1 Manufacturing Example 2 Manufacturing Example 3 Comparative Production Example 1 Comparative Production Example 2 Comparative Production Example 3 Compared to Gallic acid
Total phenol content
(Mg gallic acid/g) 11.316 11.75 8.096 8.004 10.554 1.900

As can be seen in Table 5, Preparation Examples 1 to 3 of the composite extract of the present invention was confirmed to contain a large amount of phenolic compounds. Therefore, the complex extract of the present invention may have a more advantageous effect in improving skin conditions, such as increasing skin moisturization and lowering sebum and angular mass.

Test Example 6: Evaluation of skin improvement degree

When the serum of Example 2 containing the complex extract of the present invention and the serum of Comparative Example 2 not containing the same were applied to the skin, the degree of change in skin moisturization, the amount of sebum, and the degree of each mass change were evaluated. And sensory evaluation for the sense of efficacy was performed. Each moisturizing degree, sebum amount, and angular mass were evaluated using a wireless portable skin diagnostic device (product name: dpViso, manufacturer: CHOWIS) and calculated according to the measurement index built into the device.

(1) Evaluation of the degree of change in moisture content

Twenty men and women in their 20s to 30s were allowed to use the same amount of the serum of Example 2 and the serum of Comparative Example 2 in the morning and evening for 10 days, and then the change in skin moisture content was measured at intervals of two hours on the last day. The average values of the measured values are shown in Table 6.

Immediately after use
2 hours
4 hours
6 hours
Moisture reduction rate (%) Example 2 50.63 39.42 37.58 35.90 29.05 Comparative Example 2 54.1 43.9 38.07 34.53 36.17

As shown in Table 6 and FIG. 5, it can be seen that the serum containing the complex extract of the present invention has a smaller moisturizing reduction rate (100-moisture retention rate) compared to the serum not containing it, and has the effect of maintaining moisture.

(2) Evaluation of the degree of sebum amount change

Twenty men and women in their 20s to 30s were allowed to use the serum of Example 2 and the serum of Comparative Example 2 at the same amount, respectively, once in the morning and once in the evening for 10 days, and then the change in the amount of sebum of the skin was measured. Table 7 shows the average values of the measured values.

Before use After 10 days of use sebum
Reduction rate (%) Example 2 14.43 8.14 43.6 Comparative Example 2 26.22 25.22 3.81

As shown in Table 7 and FIG. 5, it can be seen that the serum containing the complex extract of the present invention has the effect of remarkably increasing the sebum amount reduction rate compared to the serum not containing it.

(3) Evaluation of the degree of angular mass change

Twenty testers of men and women in their 20s to 30s were allowed to use the serum of Example 2 and the serum of Comparative Example 2 at the same amount for 10 days, once in the morning and once in the evening, respectively, and then the angular mass change of the skin was measured. Table 8 shows the average value of the measured values.

Before use After 10 days of use corneous
Reduction rate (%) Example 2 19.4 8.71 55.2 Comparative Example 2 13.33 8 40.0

As shown in Table 8 and Figure 5, it can be seen that the serum containing the complex extract of the present invention has the effect of lowering the angular mass compared to the serum not containing it.

(4) sensory evaluation

Twenty testers of men and women in their 20s to 30s were allowed to use the serum of Example 2 and the serum of Comparative Example 2 at the same amount for 10 days, once in the morning and once in the evening, and then applied according to the 5-point scale method, absorption power, moisturizing power, Sensory evaluation was conducted for the sense of stimulation and the degree of improvement. The results are shown in Table 9 below by averaging the total score.

Example 2 Comparative Example 2 Spreadability 4.6 4.2 absorptivity 4.4 4.2 Moisturizing power 3.9 3.6 Irritation 0 0 Degree of improvement 3.6 3.2

As shown in Table 9 and FIG. 6, it was confirmed that the serum containing the complex extract of the present invention has superior feeling of use compared to the serum not containing it.

Although the embodiments of the present invention have been described above, the present invention is not limited to the above embodiments, but may be implemented in various different forms, and those of ordinary skill in the technical field to which the present invention pertains to the technical idea of the present invention. It will be appreciated that it can be implemented in other specific forms without changing any or essential features. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting.

Claims (8)

50 ug/ml or more of the active ingredient of the complex extract of gold kiwi skin and pomegranate skin Containing in a concentration, the combination weight ratio of the gold kiwi peel extract and the pomegranate peel extract of the complex extract is 1: 0.5 to 1: 2, a cosmetic composition having anti-wrinkle effect.
The cosmetic composition of claim 1, wherein the composition further has at least one effect of anti-inflammatory, skin moisturizing, sebum reduction, dead skin cell reduction, and antioxidant activity.
delete The cosmetic composition of claim 1, wherein the composite extract is contained in an amount of 0.001% to 60.0% by weight based on the total weight of the cosmetic composition.
The cosmetic composition of claim 1, which is one of a lotion, serum, essence, lotion, cream, powder, foundation, pack, patch, mask sheet, gel ointment, spray, and detergent.
It includes mixing gold kiwi skin extract and pomegranate skin extract, or mixing dried gold kiwi crushed product and dry pomegranate peel crushed product and then extracting to prepare a complex extract, and containing it at a concentration of 50 ug/ml or more based on the total composition As a method for producing a cosmetic composition having an effect of improving wrinkles, the blending weight ratio of the gold kiwi skin extract and pomegranate skin extract or the blending weight ratio of the dry gold kiwi crushed product and the dry pomegranate peel crushed product is 1: 0.5 to 1: 2, A method for producing a cosmetic composition having anti-wrinkle effect.
delete According to claim 6, After adding 1 to 100 times the weight of the solvent to the dry raw material pulverized material to be extracted, heat extraction for 30 minutes to 360 hours at a temperature range of 10 ℃ to 120 ℃, reflux cooling extraction, ultrasonic extraction , Cold needle extraction and percolation (percolation) comprising performing one or more of, a method for producing a cosmetic composition having anti-wrinkle effect.

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