KR101606393B1 - Cosmetic composition containing scoria - Google Patents

Abstract

More particularly, the present invention relates to a cosmetic composition containing a volcanic stone clay, and more particularly to a cosmetic composition containing a volcanic stone clay as an active ingredient to inhibit 5α-reductase activity, thereby promoting hair growth, And a cosmetic composition which provides an aging effect.

Description

[0002] Cosmetic composition containing scoria,

The present invention relates to a cosmetic composition containing a volcanic stone clay, and more particularly, to a cosmetic composition containing a volcanic stone clay as an active ingredient to promote hair growth and promote anti-aging effects by promoting the synthesis of collagen .

5α-Reductase exists in male hormone-reactive tissues such as sebaceous glands, hair follicles, prostate, and epididymis, and reduces testosterone, one of the male hormones, to dihydrotestosterone (DHT) The enzyme involved is NADPH in its conversion. (Diane et al., J. Invest Dermatol., 775-778, 1995. Bruchovsky, N., et al., ≪ RTI ID = 0.0 > et al; JBC 243, 2112-2121, 1968). In addition, in the sebaceous gland of the skin, testosterone is converted to dihydrotestosterone by 5α-reductase, which binds to a receptor protein in the cytoplasm and enters the nucleus to activate sebaceous gland cells, thereby promoting differentiation, (Diane et al., J. Invest. Dermatol. 105: 209-214). Excess secreted sebum causes acne and male pattern hair loss. In particular, many hair follicles provide a cause for hair loss because dihydrootestosterone binds better to the hormone receptor than testosterone.

Active oxygen species, in particular, act as causative agents of skin aging. Skin is exposed to ultraviolet rays to produce active oxygen species, and photochemical reactions continue to occur. These reactive oxygen species lead to skin cell and tissue damage. They break down the antioxidant defense network composed of antioxidant enzymes and non-enzymatic antioxidants, so that the oxidant / antioxidant balance tends toward the oxidation state. As a result, the ongoing oxidative stress may be caused by lipid peroxidation, protein oxidation, activation of proteolytic enzymes that break down skin constituents, chain cleavage and abnormal cross-linking of elastic fibers, collagen elastin, cleavage of the hyaluronic acid chain, Causing damage to biological components such as DNA oxidation. Damage to these biocomponents causes various aging phenomena such as wrinkle formation, elasticity reduction, and pore enlargement.

Accordingly, the inventors of the present invention have found that a volcanic stone clay can inhibit the activity of 5? -Reductase and increase the production of collagen, thereby completing the present invention.

Accordingly, an object of the present invention is to provide a cosmetic composition having a hair growth effect while preventing hair loss.

Another object of the present invention is to provide a cosmetic composition having an anti-aging effect by promoting the production of collagen.

To achieve the above object, the present invention provides a cosmetic composition for accelerating hair growth comprising a volcanic stone scoria as an active ingredient.

Further, the present invention provides a cosmetic composition for anti-aging comprising a volcanic stone clay as an active ingredient.

The cosmetic composition provided by the present invention contains a volcanic stone clay to inhibit the activity of 5α-reductase when applied to the skin, thereby providing a hair loss reduction effect and a hair growth promoting effect, and also promotes collagen synthesis to prevent wrinkle formation And provides skin elasticity and anti-aging effects.

In the present specification, the term " promoting hair growth " means not only promoting the generation of new hair but also allowing the existing hair to grow healthily, and the phenomenon in which the hair falls off the scalp and the hair becomes thin And suppression.

The present invention provides a cosmetic composition for accelerating hair growth and anti-aging comprising a volcanic stone scoria (Scoria) as an active ingredient.

The volcanic stone (Scoria) used as an active ingredient in the present invention is a kind of volcanic ash present in Jeju Island in Korea. It is known that it is produced when a parasitic volcanic eruption occurs throughout Jeju Island. The reserves in Jeju Island are about 20 billion m ³ Is estimated to be almost infinite resources. The volcanic stone pine is a natural material that has the capability of eliminating E. coli bacteria from 92 to 93% of far-infrared radiation, 97% of deodorization rate, and 99.9% of antibacterial fungi in its natural state. It is not comparable to jade, ocher, elvan and germanium It is a natural material with the best features.

The volcanic stone clay of the present invention can be obtained by a method common in the art, and the production method thereof is not particularly limited. Specifically, for example, the clones collected in an area free from contamination with pesticides can be obtained by variously grinding the particle size depending on the application to remove impurities and sterilization by dry heat at a temperature of 150 ° C or higher.

The cosmetic composition according to the present invention may further comprise at least one selected from the group consisting of Cryptomeria japonica extract, Brassica camperstris (rapeseed flower) extract, bamboo (Bambusa vulgaris) extract and green tea (Camellia sinensis leaf) ≪ / RTI >

The cedar (Cryptomeria japonica) used in the present invention grows well in a valley having an average annual temperature of 12 to 14 ° C and a rainfall of 3,000 mm or more. The height is 40 m and the diameter is 1 to 2 m. The bark is reddish brown, cracked vertically, and branches and leaves are closely packed to form a conical shape. Leaves are curved needle-shaped, arranged in a spiral shape, and do not fall when dry. The flower is born in March. The male flower is gathered at the end of the small branch, and the female flower is in the shape of a ball. It runs on the end of the short branch and has a purple-colored green can. The fruit is lumpy, woody, almost round and 2 to 3 cm long. Fruit pieces are thick and have sharp projections at the ends. The seeds are 2 ~ 6 pieces in the bottom part of the fruit, long elliptical, and narrow wings. Cedar has various variations depending on the color and cracking state of the bark, the shape of the bark, the angle of the branch, the color, shape and length of the leaf, the degree of bending, Horticultural varieties and planting varieties are available, and there are many varieties of legends that have long traditions.

The rapeseed flowers (Brassica camperstris (rapeseed) flower) used in the present invention are mustard and biennial grasses. Height is 80 ~ 130cm, surface is smooth and green. In the main stem, there are about 15 primary branches and two to four secondary branch branches. The leaves are bushy and the ends are dull. The leaf on the lower stem has long petiole and the edge of leaf is deeply cracked. The leaf on the upper stem has no petiole, it surrounds the stem and its tip is thin. Leaves of western species are thick and swollen (leather-like texture), the species is light green and the leaves are undrained. The stem usually has 30 to 50 leaves. Seeds contain 38 ~ 45% of oil, 15 ~ 20% of soluble nitrogen and 20% of protein. It is consumed next to soybean oil. It is used as feed or fertilizer. The flower is a herbaceous plant, and the fatty oil separated from the seed is used as the ointment base, the solvent for the oil injection, and the lubricant for the machine.

The bamboo (Bambusa vulgaris) used in the present invention is a generic term for perennial plants belonging to the bamboo family (Bambusoideae) of the monocotyledonous plant (Poaceae). In some cases, only the tall family is called bamboo. It is distributed all over the world, but it grows mainly in the tropics, especially in the monsoon regions of Asia. In Korea, it is distributed widely in the central and southern parts of Jeju Island. In the case of a tall crow, it grows up to 30m in height and 30cm in diameter. The stem is straight, round and hollow. The growth method is that the stem in the ground stretches sideways and naturally breeds by sprinkling root and net in the nodule. Leaves are narrow and long. It likes wet land and grows fast. In addition, it does not bloom rarely, but when it is piled, it blooms all the bamboo field together. Bamboo flowers are a kind of mutation that is not related to the propagation of bamboo, and it is also called a flower vase or natural habitat. The flowering time varies from 3 years, 4 years, 30 years, 60 years, 120 years, and all bamboo fields are blossomed at the same time. Bamboo is widely used for various purposes ranging from construction materials, furniture, fishing rods, plant supports, to bamboo crafts such as baskets, and is planted for ornamental purposes. The young child eats and cooks with herbs.

In addition, the green tea (Camellia sinensis leaf) used in the present invention is obtained by treating the oxidizing enzyme present in the leaves of camellia sinensis (Camellia sinensis) with fire or steam by inactivating the enzyme . Green tea has not only been consumed as a symbolic tea since BC, but recently its value has been re-recognized as the pharmacological mechanism of the various ingredients in green tea is gradually found out. It has been found that the active ingredients such as polyphenol, caffeine, amino acid, and vitamin C are effective in the action of hangover and nicotine detoxification, fatigue recovery, arteriosclerosis, skin care and the like. Especially, , Anticancer activity, blood cholesterol lowering effect, anti-aging action, heavy metal detoxification action, tooth decay prevention and bad breath removal action.

The cedar extract, rape flower extract, bamboo extract, and green tea extract of the present invention can be prepared by extraction by a method known in the art, and the method is not particularly limited. Preferably, extraction can be carried out using an extraction solvent in which water and 1,3-butylene glycol are mixed at a weight ratio of 1: 1. At this time, the extraction temperature is preferably 40 ° C., and the extraction can be performed for 6 to 24 hours. If the extraction temperature and the extraction time are exceeded, the extraction efficiency may be deteriorated or the component may be changed.

The cosmetic composition according to the present invention contains 0.0001 to 80.0% by weight, more preferably 0.01 to 50.0% by weight, based on the total weight of the composition, of a volcanic stone flour, a cedar wood extract, a rape flower extract, a bamboo extract and a green tea extract. If the content is less than 0.0001% by weight, the effect or effect is insignificant on the above components, and if it exceeds 80.0% by weight, the increase of the effect is not so large as the content is increased.

The cosmetic composition provided by the present invention inhibits 5α-reductase activity which promotes the production and activity of dihydrootestosterone which induces hair loss when applied to the skin, thereby promoting hair hair growth and preventing hair loss . In addition, it is possible to alleviate the scalp irritation such as reduction of irritation of the scalp, such as itching and stiffness, and alleviation of inflammation of the scalp, and the scalp stimulus also includes irritation which may occur in chemical procedures such as dyeing and perming. In addition, the composition of the present invention can strengthen hair roots and prevent hair from being tapered due to scalp damage.

In addition, the cosmetic composition provided by the present invention promotes collagen synthesis when applied to skin, thereby increasing skin elasticity and suppressing wrinkle formation, thereby preventing skin aging.

The cosmetic composition according to the present invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. It may be any formulation suitable for topical application, for example, as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, Or in the form of a cream, skin, lotion, powder, ointment, spray, pack or conical stick. May also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

In addition, the composition for promoting hair growth of the present invention is not particularly limited in its formulation, but may be applied to cosmetic compositions having formulations such as hair tonic, hair nutritional lotion, scarf treatment, hair treatment, hair rinse, hair shampoo, Lt; / RTI >

In addition, in the respective formulations, the ingredients other than the above-mentioned essential ingredients can be suitably selected and blended by those skilled in the art depending on the kind of the external preparation, the purpose of use, and the like. For example, the cosmetic composition according to the present invention may be formulated into a cosmetic composition containing a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, As used herein refers to any of those commonly used in emulsifying or emulsifying agents, fillers, sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics Adjuvants commonly used in the cosmetics or dermatological fields, such as other ingredients. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to test examples and examples. However, these test examples and examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited thereto.

[Test Example 1] 5 alpha -Reedtoise activity inhibitory effect

Sprague-Dawley male rats (7-8 weeks old) were killed with diethyl ether, the abdominal prostate was removed, the connective tissue was removed, and a buffer (0.32 M sucrose, 0.1 mM dithiothreitol, 20 mM sodium acetate) was added, and the mixture was finely cut and suspended in a pulverizer. The suspension was centrifuged and the supernatant was taken to partially refine 5α-reductase, and the activity of the enzyme was inhibited.

A portion of the supernatant obtained above is placed in a buffer containing 0.2 M base acid and 0.2 M dibasic acid, and then reacted with a substrate (testosterone) with an isotope, ³ H, to measure the amount of product dihydrootestosterone Respectively. The reaction solution was added with 1 mM dithiothreitol, 40 mM sodium phosphate (pH 6.5), 50 μM NADPH, [1,2,6,7-3H] testosterone / testosterone (2.2 × ^{10 9} moles) and enzyme suspension The volume was adjusted to 565 占 퐇.

The sample was dissolved in 10% ethanol so as to be 0.1%, and 5.65 L of the above reaction solution was added to make the final concentration to 0.001%. The same volume of solvent was used as a control, and riboflavin was used as a positive control.

As a sample used, a raw material processed into powder form was used as a sample of a volcanic stone (product name: volcanic stone pine, manufactured by Biorand Co., Ltd.), cedar extract, bamboo extract, green tea extract and rape flower extract (manufactured by Bio Land Co., Was prepared by adding 100 g of the dry weight of each sample to each flask, cooling and extracting with 3 l of distilled water for 1 day, and then removing the residue by filtration.

The reaction was initiated with the addition of the enzyme suspension and proceeded at 37 DEG C for 30 minutes, followed by extraction with 1 mL of ethyl acetate. 100 mu l of the ethyl acetate phase was applied onto a silica plastic sheet kiesel gel 60 FEP 254 F254) developed with acetone-cyclohexane (1: 1).

After drying the plastic sample in air, the bath system was used to measure the amount of isotope. The dried plastic sheet and x-ray film were put together in a bath cassette. After 1 week, the testosterone and dihydrootestosterone isotope Lactation was measured. The results are shown in Table 1 below.

sample T (dpm) DHT (dpm) Conversion Rate (%) Inhibition rate (%) Volcanic ash 8750 2873 22.4 53.3 Volcanic stone cluster + cedar (1: 1) 9150 2890 21.1 56.04 Volcanic stone clay + rape flower extract (1: 1) 9320 2930 19.7 58.9 Volcanic stone clay + bamboo extract (1: 1) 9280 2910 19.2 60.0 Volcanic stone pine seed + green tea extract (1: 1) 9420 2940 20.2 57.9 (1: 1: 1: 1: 1) of volcanic stone clover + cedarwood extract + rape flower extract + bamboo extract + green tea extract 9830 2970 18.7 61.0 Control group 7430 5935 48.0 - Positive control group 7500 2530 29.7 38.1

(1) T: Radioactivity in the testosterone area.

(2) DHT: 3H radioactivity in the dihydrotestosterone region

(3) Conversion rate: Radiation to DHT area / Total radioactivity

(4) Inhibition rate: 100 * (Conversion rate of the control group - Conversion rate of the sample) / Conversion rate of the control group

From the results shown in the above Table 1, the volcanic stone clusters of the present invention convert testosterone into dihydrotestosterone, bind to the receptor protein in the cytoplasm, enter the nucleus, activate the sebaceous gland, promote differentiation, It was found that the inhibition of the activity of dactate was blocked effectively by blocking the conversion of testosterone to dihydrotestosterone.

In addition, it was confirmed that when the volcanic stone clusters were combined with the cedar, rape, bamboo, green tea extracts or mixtures thereof, the 5α-reductase activity inhibitory effect was higher than that when the volcanic stone clusters alone were used.

Therefore, the mixture of the volcanic stone clusters and the volcanic stone clusters of the present invention and the mixture of the cedar wood extract, rape flower extract, bamboo extract or green tea extract is effective for inhibiting the hypersecretion of sebum due to its excellent 5α-reductase activity inhibitory effect.

[Referential Example 1]

Lotion Formulations Examples 1 to 6 and Comparative Example 1 were prepared according to the composition shown in Table 2 below (unit: wt%).

Raw material name Example
One Example
2 Example
3 Example
4 Example
5 Example
6 Comparative Example
One 1. Cetearyl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 1.0 2. Chin type glyceryl stearate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 3. Glyceryl stearate SE 1.5 1.5 1.5 1.5 1.5 1.5 1.5 4. Phyto squalane 3 3 3 3 3 3 3 5. Hydrogenated polydecene 2 2 2 2 2 2 2 6. Dimethicone 0.5 0.5 0.5 0.5 0.5 0.5 0.5 7. Polysorbate 60 One One One One One One One 8. Sorbitan sesquioleate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 9. Methylparaben 0.1 0.1 0.1 0.1 0.1 0.1 0.1 10. Propylparaben 0.05 0.05 0.05 0.05 0.05 0.05 0.05 11. Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 12. Butylene glycol 5 5 5 5 5 5 5 13. Polyacrylate-13 * Polyisobutene * Polysorbate 20 0.5 0.5 0.5 0.5 0.5 0.5 0.5 14. Volcanic stone clay One - - - - - - 15. Volcanic stone clay + cedar extract (1: 1) - One - - - - - 16. Volcanic stone clay + rape flower extract (1: 1) - - One - - - - 17. Volcanic stone pine bark + bamboo extract (1: 1) - - - One - - - 18. Volcanic stone pine mushroom + green tea extract (1: 1) - - - - One - - 19. Volcanic stone pine tree + Cedarwood Extract + Rape Blossom Extract + Bamboo Extract + Green Tea Extract (1: 1: 1: 1: 1) - - - - - One -

≪ Preparation methods of Examples 1 to 6 and Comparative Example 1 >

1) The components 11 to 19 were uniformly mixed while heating to 70 占 폚 to prepare a water-based part.

2) The above components 1 to 10 were uniformly mixed while heating to 70 ° C to prepare an oil phase part.

3) The oil phase part of 2) was put into the water phase part of the above 1) and homomixed at 7,200 rpm for 6 minutes.

4) The mixture of 3) was cooled to room temperature.

[Test Example 2] Stimulation relaxation efficacy test

To evaluate the efficacy of the stimulant mitigation treatment by stimulation of the hair dye, the MEST method using a mouse was applied and the test method was as follows.

Six 7 - week - old BALB / c rats were purchased and divided into 7 groups. The test group was composed of 10 days of quarantine and adaptation period. The hair was removed from the abdomen of the rats and 2.5% para-phenylenediamine (PPD) was applied for 3-5 days. After a one-week rest period, the hair dye was applied to the rat's ear and wiped off after 30 minutes. After a resting period of one week, the compositions of Examples 1 to 6 and Comparative Example 1 were applied twice at intervals of one hour. After applying the hair dye again, the compositions of Examples 1 to 6 and Comparative Example 1 were applied twice after 24 hours. After 24 hours, the weight of the ear was measured, and the weight of the ear that was pathologically organized by H & E staining was measured. The formulation of the hair dye is shown in Table 3 below, and the measurement results are shown in Table 4 below.

ingredient Content (% by weight) 1 Component Purified water To 100 Sodium sulfite 0.5 Ascorbic acid 0.5 Polyoxyethylene < / RTI > 4.0 Polyoxyethylene oleyl ether 4.0 Cetostearyl alcohol 8.0 p-phenylenediamine 0.5 Resorcinol 0.5 Monoethanolamine Suitable amount ammonia Suitable amount 2 ingredients Purified water 0.1 Hydrogen peroxide (30%) 17.0 Phosphoric acid 0.1

Treated group Ear Weight (mg) Ear thickness (㎛) Example 1 9.75 + - 0.943 266.12 ± 24.568 Example 2 9.46 + 0.875 252.24 + - 22.392 Example 3 9.52 + 0.468 254.46 ± 23.493 Example 4 9.43 ± 0.678 250.08 + - 21.498 Example 5 9.55 ± 0.554 255.48 ± 24.487 Example 6 9.24 + 0.721 224.24 ± 21.792 Comparative Example 1 14.39 + - 0.242 322.75 ± 21.883

As can be seen from Table 4, it can be seen that the effect of pretreatment with the compositions of Examples 1 to 6 according to the present invention is significantly reduced ear weight and ear thickness, thereby alleviating inflammation. Thus, it can be concluded that the composition according to the present invention has an effect of relieving inflammation caused by a hair dye.

[Test Example 3] In vivo test for suppressing itching

The itching inhibiting effect of the composition according to the present invention was tested. Hairless mice were used to facilitate application of the compositions of Examples 1 to 6 and Comparative Example 1 to the skin. All mice were 7 - week - old males and 8 - reared mice were used in all groups for comparison with negative controls.

The treatment with the external preparation was carried out by taking 150 占 퐇 of the compositions of Examples 1 to 6 and Comparative Example 1 and spreading them widely on the back region of the hairless mice. Morning and afternoon, twice a day for 1 day, and 2 days for the last treatment 30 minutes before the injection of the itch inducer.

The itching test gave a 30-minute adaptation time to the obsessive-to-obese mice, 30 minutes before the injection of the itch inducer. At the end of the adaptation period, compound 48/80 (compound 48/80), which is an itch inducing substance, was dissolved in physiological saline at a concentration of 1 mg / ml, and then intradermal injection was performed on the back skin of the adaptation test hamster . Immediately after the injection, the rats were placed in the observer for 30 minutes, and the number of scratches on the area where the itch inducing substance was injected into the hind legs was measured and displayed as a number. Except for the scratching or bite of the paws, only the scratching of the injection site with the hindpaw was regarded as an index of the itching behavior. The measurement results are shown in Table 5 below.

Treated group Itching substance Average number of scratches for 30 minutes Negative control group Saline solution 16.7 ± 27.9 Example 1 C48 / 80 122.4 ± 24.8 Example 2 C48 / 80 120.3 ± 21.5 Example 3 C48 / 80 119.7 ± 22.4 Example 4 C48 / 80 121.2 ± 22.5 Example 5 C48 / 80 118.6 ± 20.4 Example 6 C48 / 80 110.2 ± 18.5 Comparative Example 1 C48 / 80 141.8 ± 21.5

As can be seen from Table 5, it can be seen that the pretreatment with the compositions of Examples 1 to 6 according to the present invention has an effect of suppressing the itching by decreasing the number of scratches.

Thus, it can be seen that the composition according to the present invention can improve the scalp condition as a result of the above test for relieving inflammation and suppressing skin itching symptoms.

[Test Example 4] Test for preventing hair loss

Seven subjects with an initial hair loss phenomenon were subjected to an experiment on the effect of preventing hair loss for 12 weeks. The compositions of Examples 1 to 6 and Comparative Example 1 were used once a day for 12 weeks. After 12 weeks had elapsed, the hair was collected again when hair was wound and counted. The results are shown in Table 6 below.

Treated group Number of dropouts Example 1 62 Example 2 60 Example 3 59 Example 4 59 Example 5 60 Example 6 55 Comparative Example 1 69

As can be seen from Table 6, when the compositions of Examples 1 to 6 according to the present invention were treated, it was confirmed that the number of decay moths was significantly reduced as compared with that of the composition of Comparative Example 1.

[Test Example 5] Hair strength test

The hair strengthening effect of the composition according to the present invention was tested.

The compositions of Examples 1 to 6 and Comparative Example 1 were treated with the same amount of experimental tress hair, dried at room temperature for 30 minutes, and hair having a thickness of 80 to 90 탆 was collected from the dried test hair. The traced hairs were cut 10cm apart, hanging paper weights at both ends, hanging 7cm above the floor, balancing both sides, and measuring the distance between both ends of the experimental horseshoe hair with a burr caliper. After 15 experiments with hair, the distance of the average was calculated. The results are shown in Table 7 below.

Treated group Hair distance (cm) Example 1 2.50 Example 2 2.52 Example 3 2.51 Example 4 2.52 Example 5 2.53 Example 6 2.56 Comparative Example 1 2.10

As can be seen from Table 7, when the composition of Examples 1 to 6 according to the present invention was treated, the hair distance was prolonged and it was confirmed that there was an effect of generating force on the hair.

[Test Example 6] Hair growth effect test

The hair growth effect of the composition according to the present invention was tested.

7-week-old mice (C57BL / 6) were removed from the dorsal hairs and the back skin was clean. Seven mice were selected for each material group, and the compositions of Examples 1 to 6 and Comparative Example 1 were administered to each mouse group 150 占 퐇 / individual was applied for 21 days. As the negative control, 0.5% by weight of Peony root extract was dissolved in a solvent composed of water / ethanol / 1,3-butylene glycol (5/3/2) and used.

The weight of newly emerged hair was measured and compared with the negative control group. The results are shown in Table 8 below.

Treated group Hair Weight (mg) Negative control group 26 ± 15 Comparative Example 1 60 ± 9 Example 1 76 ± 12 Example 2 78 ± 11 Example 3 79 ± 12 Example 4 76 ± 10 Example 5 77 ± 11 Example 6 80 ± 10

As can be seen from Table 8, when Examples 1 to 6 according to the present invention were applied, the average value of hair weight was higher than that of the normal solution (water / ethanol / 1,3-butylene glycol) And it promoted the transition to the growing hair cycle period by acting on the mouse of the resting hair cycle period, and it was confirmed that there is an effect of promoting excellent hair growth.

Therefore, when hair roots are strengthened, force is generated on hair, hair loss is prevented, and hair growth is effective. From the test results, it can be seen that the composition according to the present invention has an effect of strengthening hair roots. In addition, it is confirmed that the effect of promoting hair growth and preventing hair loss is excellent, and that hair growth promoting effect is excellent.

[Test Example 7] Promotion of collagen biosynthesis

The collagen biosynthesis promoting effect of a mixture of volcanic ash and volcanic ash, cedar, rape flower, bamboo extract and green tea extract was compared with that of tocopherol and EGCG.

First, fibroblasts were seeded at 10 ⁵ per well in 24 wells and cultured until they grew to about 90%. After culturing for 24 hours in serum-free DMEM medium, the volcanic stone, cedar, rape, bamboo, green tea extract, tocopherol and EGCG of the present invention dissolved in serum-free medium were treated at a concentration of 10 ^-4 mol, CO ₂ Lt; / RTI > The supernatant was removed and the procolagen (I) ELISA kit (procollagen type (I)) was used to determine whether procollagen was increased or decreased. The results are shown in Table 9, and the combined performance of the collagen was set to 100 in the non-treatment group.

division Collagen Total Performance (%) Untreated group 100 Tocopherol 113 EGCG 120 Volcanic ash 125 Volcanic stone cluster + cedar (1: 1) 129 Volcanic stone clay + rape flower extract (1: 1) 126.5 Volcanic stone clay + bamboo extract (1: 1) 127 Volcanic stone pine seed + green tea extract (1: 1) 128 (1: 1: 1: 1: 1) of volcanic stone clover + cedarwood extract + rape flower extract + bamboo extract + green tea extract 131

From the results shown in Table 9, it can be seen that the volcanic stone clusters according to the present invention have higher collagen aggregation performance than the positive control groups, tocopherol and EGCG, and that the volcanic stone clusters, cedarwood extract, rape flower extract, bamboo extract, It was confirmed that when the mixture was used together, the collagen aggregation performance was further improved.

Therefore, the mixture of the volcanic stone clusters and the volcanic stone clusters of the present invention and the mixture of the cedar, the rapeseed, the bamboo, or the green tea extract can increase the amount of collagen around the pores, thereby reducing the enlarged pores.

[Test Example 8] Evaluation of wrinkle improvement effect

In order to examine the wrinkle-reducing effect of Examples 1 to 6 and Comparative Example 1, the following experiment was conducted. 70 women in their 20s and 40s were divided into seven groups each consisting of 10 individuals. The lotions of Examples 1 to 6 and Comparative Example 1 were applied to the eye area designated for 8 weeks twice a day, morning and evening, respectively, and then a silicone replica ), And the state of the wrinkles at the designated site was measured with a visiometer (SV60, Courage + Khazaka electronic GmbH, Germany) as an image analyzer. The results are shown in Table 10 below, which shows the average of the parameter values after 8 weeks minus the parameter values 8 weeks before. That is, the negative value indicates that the wrinkle improving effect is higher.

division R1 R2 R3 R4 R5 Example 1 -0.31 -0.20 -0.13 -0.09 -0.08 Example 2 -0.33 -0.22 -0.16 -0.09 -0.09 Example 3 -0.32 -0.23 -0.15 -0.10 -0.10 Example 4 -0.34 -0.21 -0.17 -0.12 -0.09 Example 5 -0.35 -0.25 -0.18 -0.11 -0.11 Example 6 -0.40 -0.29 -0.25 -0.16 -0.14 Comparative Example 1 0.06 0.05 0.02 -0.01 0.00

R1: Difference between the maximum value and the minimum value of the wrinkle contour line

R2: The wrinkle contour line is arbitrarily divided into 5 squares, and the average of R1 values

R3: Maximum value of R1 divided by 5

R4: Average value obtained by subtracting the value of each valley and valley from the baseline of the wrinkle contour line

R5: Mean value of the value obtained by subtracting the outline of each wrinkle from the base line of the wrinkle contour line

As shown in Table 10, Examples 1 to 6 according to the present invention were found to be superior to Comparative Example 1, in which nothing was included, to improve skin wrinkles.

[Test Example 9] Evaluation of skin stability

In order to confirm the skin safety of the composition of the present invention, a patch test was carried out in which four adult women and four men were applied (average 34.5 years old) to Examples 1 to 6 and Comparative Example 1, Stability was evaluated.

Twenty - eight hours after the application of the patch, the patch was removed, and the first reading was performed 30 minutes later and the second reading after 96 hours. To determine the intensity of the skin irritation of the sample, weights were assigned according to the degree of the positive reaction of the skin, and the average skin irritation degree of the skin was visually determined. The results are shown in Table 11 below.

Test substance Average reactivity Judgment grade Example 1 0.000 No stimulation Example 2 0.000 No stimulation Example 3 0.000 No stimulation Example 4 0.000 No stimulation Example 5 0.000 No stimulation Example 6 0.000 No stimulation Comparative Example 1 0.100 Stimulation

From the results of Table 11, it can be seen that all of Examples 1 to 6 do not stimulate the skin.

Therefore, the cosmetic composition of the present invention can be judged to have excellent skin stability.

Claims (6)

A cosmetic composition for promoting hair growth comprising a volcanic stone scoria as an active ingredient,
The composition may further comprise at least one selected from the group consisting of Cryptomeria japonica extract, Brassica camperstris (rapeseed flower) extract, bamboo (Bambusa vulgaris) extract, and green tea (Camellia sinensis leaf) Wherein the hair growth promoting composition is a cosmetic composition for enhancing hair growth. delete The method according to claim 1, wherein the volcanic rock clusters; And at least one member selected from the group consisting of cedarwood extract, rape flower extract, bamboo extract, and green tea extract is contained in an amount of 0.0001 to 80.0% by weight based on the total weight of the composition. delete The cosmetic composition for promoting hair growth according to claim 1, wherein the composition is for inhibiting the activity of 5? -Reductase. delete