KR101245813B1 - Cosmetic composition comprising lycii fructus extract and uncaria tomentosa extract for whitening of the skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening, and more particularly, to a cosmetic composition for skin whitening comprising Gojija (Lycii fructus) extract and cat claw (Uncaria tomentosa) extract as an active ingredient.

The cosmetic composition according to the present invention is effective in improving skin whitening or bleeding freckles by inhibiting melanin production and preventing pigmentation, and has almost no skin side effects, and thus can be used safely.

Wolfberry, Cat's Claw, Whitening, Cosmetics

Description

Cosmetic composition for skin whitening containing Goji berry extract and cat's claw extract {COSMETIC COMPOSITION COMPRISING LYCII FRUCTUS EXTRACT AND UNCARIA TOMENTOSA EXTRACT FOR WHITENING OF THE SKIN}

The present invention relates to a cosmetic composition for skin whitening, and more particularly, to a cosmetic composition which is effective in improving skin whitening or freckles and reducing freckles by preventing melanin production and preventing pigmentation.

Human skin color is determined by the concentration and distribution of melanin in the skin. In addition to genetic factors, it is also influenced by environmental conditions such as ultraviolet rays, physiological conditions such as fatigue and stress.

Melanin is produced by non-enzymatic oxidation after tyrosinase acts on tyrosine, a kind of amino acid, into dopa and dopaquinone. Although such a melanogenesis pathway is known, the mechanism of inducing melanin synthesis, the previous step in which tyrosinase works, is not yet clear.

When too much melanin is produced in the skin, it can darken the skin color and cause blemishes and freckles. Therefore, by inhibiting the synthesis of melanin pigment in the skin can not only brighten the skin color to realize skin whitening, but also improve skin hyperpigmentation, such as blemishes, freckles, etc. caused by ultraviolet rays, hormones and genetic causes .

Conventionally, a substance having inhibitory activity against tyrosinase, such as hydroquinone, ascorbic acid, kojic acid, glutathione, etc., which is a chemical component, is mixed with cosmetics such as ointments, creams, and essences for skin to realize skin whitening, blemishes, freckles, etc. Improved skin hyperpigmentation.

However, hydroquinone has severe skin irritation and has a problem of limiting the amount of the compound to a very small amount. Ascorbic acid is easily oxidized, and the cosmetics containing the compound may cause discoloration and discoloration, resulting in deterioration of product quality and thus whitening effect. It has a downside. In addition, kojic acid is more likely to be carcinogenic when administered orally, and further safety verification is required, and thiol compounds such as glutathione and cysteine not only have a characteristic unpleasant odor, but also have problems with transdermal absorption, and glycosides and derivatives thereof. Also has a high polarity is difficult to use as a component of the cosmetic composition.

In order to solve the problems of the prior art as described above, an object of the present invention is to provide a cosmetic composition for skin whitening containing a natural extract that is effective in whitening the skin, but has little side effects.

In order to achieve the above object, the present invention provides a cosmetic composition for skin whitening comprising Gojija (Lycii fructus) extract and cat claw (Uncaria tomentosa) extract as an active ingredient.

According to the present invention, it is preferable that the cosmetic composition contains Goji berry extract and cat's claw extract in an amount of 0.1 to 30% by weight, respectively, based on the total composition.

Hereinafter, the present invention will be described in more detail.

The present inventors conducted a study on natural herbal ingredients that have a whitening effect, the gojija extract and cat's toenail extract not only shows an excellent whitening effect by the synergistic effect of each component but also confirmed that there are almost no skin side effects, based on this The invention was completed.

The cosmetic composition for skin whitening of the present invention is characterized by comprising Gojija (Lycii fructus) extract and cat claw (Uncaria tomentosa) extract as an active ingredient.

1) Wolfberry Extract

Lycii fructus is a mature fruit of Lycium chinense miller belonging to the family Solanaceae. It is a round or oval berry and is about 10 mm long and 5 mm in diameter. Goji berry contains betaine, zeazanthin, carotene, thiamine, vitamin A, vitamin B1, vitamin B2, and vitamin C. In addition, goji berries are known to be excellent in protecting the liver function, prevent adult diseases such as diabetes, improve the function of the lungs and kidneys, protect the weak constitution, and have antioxidant activity.

The wolfberry extract is prepared by extracting by a conventional method of producing a plant extract, preferably, the extract obtained by drying or grinding ground wolfberry, and using a solvent selected from the group consisting of distilled water, organic solvents and mixtures thereof. Can be.

The organic solvent may be at least one selected from the group consisting of 1,3-butylene glycol, alcohol having 1 to 4 carbon atoms, ethyl acetate, and acetone, and may be used at least two kinds sequentially according to the polarity of the organic solvent. have.

As an example of the extraction method, by adding a solvent 200 to 700 parts by weight with respect to 100 parts by weight of wolfberry, using a hot needle method to extract by heating at 50 to 100 ℃ for 1 to 24 hours, or at a low temperature of 4 to 30 ℃ Cooling method which deposits for 1 to 20 days can be used. The extract filtrate obtained at this time may be dehydrated or desolvated, and then concentrated under reduced pressure or lyophilized to obtain a liquid or powder.

2) Cat's Claw  extract

Cat's claw (Uncaria tomentosa) is a natural herb native to the Amazon jungle of Peru, South America. It grows around trees and eventually grows to 20 cm in diameter and over 30 m in length. Cat's claws are said to have been used as an effective medicine to relieve pains such as joint pain, back pain, rheumatism, etc. since the ancient Inca empire.

According to data on cat claws published by the Peruvian government, the components obtained from hot water extraction or alcohol extraction from the skin of cat claws include cancers such as bronchial asthma, bronchitis, arthritis, rheumatism, lung and neck, herpes, gastrointestinal disorders, Highly effective for pancreatitis, hepatitis, hemorrhoids. In particular, the mechanism for the improvement of joint pain and rheumatism is 'normalization of the immune system' and the other is 'pain and anti-inflammatory action'. In other words, by ingesting the cat's claw extract, the immune system is normalized, inflammation and pain are suppressed, and the flow of stagnant blood is improved, and the metabolism of muscles, ligaments, cartilage, etc.

Cat's claw extract is prepared by extracting by the conventional method of producing a plant extract as described above, preferably with a pulverized product obtained by drying or grinding the skin and roots of cat's claws, distilled water, organic solvents and mixtures thereof It can be extracted using a solvent selected from the group consisting of.

Preferred extraction method is the addition of 200 to 700 parts by weight of solvent with respect to 100 parts by weight of cat's claw, using a warming method to extract by heating for 1 to 24 hours at 50 to 100 ℃, or 1 to at a low temperature of 4 to 30 ℃ The cold acupuncture method which deposits for 20 days can be used. The extract filtrate obtained at this time may be dehydrated or desolvated, and then concentrated under reduced pressure or lyophilized to obtain a liquid or powder.

The cosmetic composition of the present invention can be added to the extract of the wolfberry and cat's claws as described above, it can be added to the mixed extract extracted with the wolfberry and cat's claws. The wolfberry and cat claw mixed extract can be prepared by a conventional plant extract manufacturing method as described above, wherein the weight ratio of wolfberry: cat claw is preferably controlled to be 1: 1.

The cosmetic composition of the present invention preferably comprises such a wolfberry extract and cat's claw extract in an amount of 0.1 to 30% by weight based on the total composition. Or, when adding the wolfberry and cat's claw mixed extract may comprise 0.1 to 30% by weight based on the total composition. That is, the content of the extract is preferably more than the minimum value to achieve a minimum skin lightening effect, and the content of the extract is preferably less than the maximum value in consideration of the deterioration of the feeling of use due to excessive addition and the applicability to various formulations. At this time, the content of the extract is preferably adjusted appropriately within the above range depending on the content of the components contained in the formulation or cosmetic composition.

The cosmetic composition of the present invention may be provided for applications such as skin external ointment, basic cosmetics, makeup cosmetics, and body cosmetics. Examples of the basic cosmetics include essences, lotions, creams, gels, lotions, packs, massage creams, emulsions, and the like; The makeup cosmetics include a foundation, a makeup base, and the like; Examples of body cosmetics include soaps, liquid detergents, bath agents, sun screen creams, and sun oils.

At this time, to improve the physical properties of the fragrance, pigments, fungicides, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, synthetic polymer materials and the like can be further added.

Hereinafter, the preparation examples and examples are presented in order to explain the present invention in more detail. However, the following Preparation Examples and Examples are merely illustrated to aid the understanding of the present invention, and may be modified in various other forms, and the scope of the present invention is not limited to the following Examples.

Manufacturing example  1: Preparation of wolfberry extract

After washing the wolfberry with water and finely chopped, 1.5 kg of 1,3-butylene glycol and 3.5 kg of purified water were added per 1 kg of wolfberry, followed by immersion extraction for 5 days, and then aged at 4 ° C for 4 days. Subsequently, the aged extract was filtered through a 0.4 μm filter, then passed through an ion exchange resin, and again filtered through a 0.2 μm filter and a 0.1 μm filter to obtain a Goji berry extract.

Manufacturing example  2: Cat's Claw  Preparation of extract

The cat's claws and roots were washed with water and finely chopped, and 1.5 kg of 1,3-butylene glycol and 3.5 kg of purified water were added per 1 kg of cat's claws, followed by immersion extraction for 5 days, and then aged at 4 ° C for 4 days. Subsequently, the matured extract was filtered through a 0.4 μm filter, then passed through an ion exchange resin, and again filtered through a 0.2 μm filter and a 0.1 μm filter to obtain a cat's claw extract.

Manufacturing example  3: wolfberry and Cat's Claw  Preparation of Mixed Extract

After washing the wolfberry, cat's claws and cat's claw roots with water, finely chop them, and add 1.5 kg of 1,3-butylene glycol and 3.5 kg of purified water per kg of wolfberry and cat's claws (weight ratio = 1: 1) for 5 days. After immersion extraction, the mixture was aged at 4 ° C for 4 days. Subsequently, the matured extract was filtered through a 0.4 μm filter, then passed through an ion exchange resin, and again filtered through a 0.2 μm filter and a 0.1 μm filter to obtain a cat's claw extract.

Experimental Example  1: Cytotoxicity test

To verify the primary safety as a raw material used in cosmetics, V79-cells (Chinese) for the wolfberry and cat's claw mixed extract, sodium lauryl sulfate (control) and squalane (control), respectively, according to Preparation Example 3 Hamster, continuous cell line of lung tissue fibroblasts) to culture MTT test (Refs. Mossman T. (1983). Rapid colorimetric assay for cellular growth & survival: application to proliferation & cytotoxicity assays. Journal of Immunological Methods 65, 55 ~ 63] was carried out to test the cytotoxicity, the results are shown in Table 1 below.

As shown in Table 1, the extract of wolfberry and cat's claws has an IC ₅₀ value of 5.000, which is 1000 times lower in cytotoxicity than sodium lauryl sulfate (SLS), and also compared to squalane, which is a useful component having excellent safety. Cytotoxicity was found to be 100 times lower.

Experimental Example  2: allergy  Likelihood Assessment

Goji berry extract and cat's claw extract were confirmed to be allergic by local lymph node assay (LLNA) [Kimber (1990). Identification of contact allergens using the murine local lymph node assay, J. Appl. Toxicol. 10 (3), 173-180].

In this case, acetone: olive oil (4: 1) was used as a carrier medium, and the mixed extract of Preparation Example 3 was adjusted to a concentration of 0%, 20%, 40%, and 60% in the carrier medium. It was. Specifically, 50 μl of each sample was applied to both ears of the mouse (Balb / c) for 3 days, and then, an isolated lymph node was isolated from the rat. Subsequently, the lymph nodes were pulverized into a single cell state, and then cultured for 24 hours with the addition of radioisotope (3H-thymidine), and then the amplification degree (cpm) of the cells was measured, and the results are shown in Table 2 below. It was.

As shown in Table 2, the wolfberry and cat's claw mixed extract was found to have almost no allergic potential as the amplification degree 2 or less.

Experimental Example  3: skin irritation test

To test skin irritation of Goji berry extract and cat's claw extract, patch test using guinea pig (Guinea Pig) was carried out (1) Draize, J.H. (1959): Dermal toxicity. Assoc. Food and Drug officials, US. Appraisal of the safety of chemicals in Food, Drugs and Cosmetics., Pp 46-59, Texas State Dept of Health, Austin, Texas. ② Federal Register (1973): Method of testing primary irritant substances 38 (187): pp 1500-1541].

At this time, the sample was used to prepare an oil-in-water type face wash adjusted to the concentration of 0%, 0.001%, 0.1%, 10% and 30% of the mixed extract of Preparation Example 3. Specifically, the hair of the sample application site (back, 1.5cm × 1.5cm) of the guinea pig is removed, and after 24 hours of environmental adaptation to minimize skin irritation, a gauze moistened with the sample is applied to the application site. Apply and seal with solid foil and fix with bandage for 48 hours to prevent evaporation and loss. The extent of stimulation was then determined at 2 hours and 24 hours (48 hours, 72 hours after patch) after closure patch removal, and the results are shown in Table 3 below.

As shown in Table 3, the mixture of wolfberry and cat's claws is very safe and showed little skin irritation even when used in excess (concentration 30%).

Experimental Example  4: primary irritation test in humans (closed) Patch )

A sample containing 1.0% of the mixed extract of Preparation Example 3, 1.0% of sodium lauryl sulfate and 10.0% of ethanol, a sample containing 1.0% of sodium lauryl sulfate (control), and a sample containing 10.0% of ethanol (control) ), A human patch test was performed on 50 healthy men and women according to CTFA guidelines (The Cosmetic, Toility and Fragrance Association. Inc. Washington, DC, 20036, 1991).

Specifically, 20 µl of the sample and the solution was added dropwise to a Fin chamber, and it was placed on the back of the human body, which is a test site, and fixed with tape. Subsequently, after patching for 24 hours, the patch was removed and after 4 hours, skin irritation of the test site was determined according to the criteria and calculation formula of Table 4 below, and the results are shown in Table 5 below.

[formula]

As shown in Table 5, the extract of wolfberry and cat's claws was found to significantly alleviate the stimulation of sodium lauryl sulfate and ethanol even at a small amount. From these results, the wolfberry extract and cat's claw extract are safe for skin. It can be seen that the ingredient.

The following are examples of preparing a cosmetic for skin whitening by adding goji berry extract and cat's claw extract to an external skin ointment, essence, lotion, cream, and gel.

Example  1 to 4 and Comparative example  1 ~ 3: skin external ointment

Example  5: essence

Example  6: lotion

Example  7: cream

Example  8: gel

Experimental Example  5: evaluation of pigmentation inhibitory effect

About the cosmetic composition of Examples 1-8 and Comparative Examples 1-3, the pigmentation inhibitory effect was evaluated by the following method.

First, 20 healthy men and women were selected, and the lower part of both arms, which were to be irradiated with light, was washed with 70% ethanol aqueous solution. Subsequently, aluminum foil (two holes with 6 holes having a diameter of 7 mm in diameter) was attached to the subject's arm, and then 60 mJ / cm 2 light quantity was obtained using ORIEL Solar Simulator (1,000 W) at a distance of 10 cm from the arm. The light was irradiated with. At this time, the cosmetic compositions of Examples 1 to 8 and Comparative Examples 1 to 3 were each applied twice a day from 3 days before light irradiation to 3 weeks after light irradiation.

To evaluate the degree of inhibition of pigmentation of the cosmetic composition according to each Example and Comparative Example visually evaluated in two stages ('effective' and 'no difference'), and examined the occurrence of skin side effects, the results are shown in the following table 6 is shown.

As shown in Table 6, Comparative Example 1 had no improvement on the degree of pigmentation, Comparative Example 2 and Comparative Example 3 were obtained by using the wolfberry extract (5% by weight) or cat claw extract (5% by weight) alone, respectively. It had a whitening effect, but it was insignificant.

In contrast, in Examples 2 to 8, in which 5% by weight of wolfberry and cat's claw mixed extracts were applied, 10 or more of 20 test subjects exhibited significant skin whitening effects, and 0.1% by weight of wolfberry and cat's claw mixed extract contents. In the case of Example 1, more than six people showed a skin whitening effect, it could be seen that no skin side effects.

As described above, the cosmetic composition for skin whitening according to the present invention, by including goji berry extract and cat's claw extract, inhibits melanin production and prevents pigmentation, thereby improving skin whitening or blemish freckles, while also having skin side effects There are few advantages that can be used safely.

Claims (4)

A cosmetic composition for skin whitening comprising 0.1 to 30% by weight of Gojija (Lycii fructus) extract and 0.1 to 30% by weight of cat's claw (Uncaria tomentosa) extract as an active ingredient. delete The method of claim 1, The wolfberry extract and the cat's claw extract, respectively or simultaneously extract the wolfberry and cat's claws with at least one solvent selected from the group consisting of distilled water, 1,3-butylene glycol, alcohol having 1 to 4 carbon atoms, ethyl acetate and acetone And a cosmetic composition for skin whitening obtained by filtration after warming or cooling. The method of claim 1, The cosmetic composition is selected from the group consisting of skin external ointment, essence, lotion, cream, gel, lotion, pack, massage cream, latex, foundation, makeup base, soap, liquid cleanser, bath, sun screen cream, and sun oil Cosmetic composition for skin whitening that has a formulation.