KR102107417B1 - Composition containing extract using process of herbal medicine - Google Patents

Abstract

The present invention relates to a composition comprising an extract of a ginseng treated with a foaming agent and an extract of a green tea with a foaming treatment.

Description

Composition comprising a foam extract {COMPOSITION CONTAINING EXTRACT USING PROCESS OF HERBAL MEDICINE}

The present invention relates to a composition comprising an extract of a ginseng treated with a foaming agent and an extract of a green tea with the foaming.

The appearance of the skin is the extracellular matrix (ECM) component of the dermal tissue, which is collagen, which accounts for about 70 to 80% of the total ECM. The formation of skin wrinkles is due to the degradation or destruction of collagen caused by aging or ultraviolet rays. In particular, due to the expression of matrix metallo protease, such as collagenase, collagen normally generated in the skin is decomposed, resulting in wrinkles.

Various substances have been developed and used for the purpose of suppressing the decrease in collagen that causes wrinkles. Retinoids such as retinol and retinoic acid exhibit wrinkle improvement effects (Dermatology therapy, 1998, 16, 357-364). , Compositions containing malt extract and the like have been applied to suppress collagenase. However, these retinoids have the disadvantage that irritation appears even when only a small amount is applied to the skin, and most natural-derived raw materials have been used in the form of simple extracts, and it is not known exactly what effect the extracts are showing due to It is a reality that it is difficult to continuously maintain and control the activity of the extract.

On the other hand, when the skin moisture content decreases, keratin due to drying often occurs. In addition, the shine of the skin disappears, fine wrinkles easily occur, and skin aging begins early. That is, it is essential to maintain skin moisture to have improved skin.

The present inventors confirmed that the mixture of the ginseng extract of the ginseng and the extract of green tea of the ginseng suppresses the formation of wrinkles on the skin or improves wrinkles, and has a much better synergistic effect than the mixture of the common ginseng and green tea extracts on moisturizing the skin. Was completed.

Dermatology therapy, 16, 357 ~ 364, 1998

It is an object of the present invention to provide a composition comprising a natural product exclusion extract.

In order to achieve the above object, the present invention provides a composition comprising a mixture of a formulation-treated ginseng extract and a formulation-treated green tea extract.

The composition of the present invention has a remarkably superior anti-aging effect or moisturizing effect than a mixture of each extract without treatment or each of the extracts treated with a formulation including a mixture of a ginseng extract treated with a formulation and a green tea extract treated with a formulation. Specifically, the composition of the present invention has a synergistic effect by mixing each of the preparation-treated extracts, thereby promoting the production of pro-collagen and suppressing the expression of collagenase. In addition, the composition of the present invention can promote the expression of filaggrin to strengthen the skin barrier function and induce differentiation of keratinocytes. The composition of the present invention also has little toxicity and irritation including the natural extract, and has little side effect even when used for a long time.

Therefore, the composition of the present invention can improve the overall condition of the skin, and can be usefully used as a composition for external application for skin to prevent or improve skin dryness, atopic dermatitis, contact dermatitis or psoriasis caused by incomplete epidermal differentiation.

The present invention relates to a composition comprising a mixture of a ginseng extract and a green tea extract treated with a formulation in one aspect.

In one aspect of the composition of the present invention, the composition includes a composition for external application for skin.

In one aspect of the composition of the present invention, the composition includes a composition for anti-aging of the skin comprising a mixture of a ginseng extract treated with a formulation and a green tea extract treated with a formulation as an active ingredient.

In one aspect of the present invention, the mixture of the ginseng extract and the green tea extract treated with the foamed agent is not only a mixed extract obtained by mixing and extracting the ginseng and the green tea, but also each of the ginseng extract and the green tea extract obtained by separately extracting the ginseng and the green tea. Mixture is also included.

In the present specification, "foje" (匍 制) refers to a pharmaceutical technology that changes the original properties of a medicine by processing the medicine based on the theory of oriental medicine. The foaming agent may include a process of boiling, steaming, roasting, roasting, or heating to reduce the toxicity of the drug, to preserve the drug, to improve the drug, and to improve the convenience of taking the drug. In addition, the preparation method includes, for example, the second method of roasting the medicinal material, the method in which the auxiliary material is impregnated into the drug tissue by roasting the medicinal material together with a certain amount of the liquid auxiliary material, and the liquid in accordance with the medicinal preparation regulations of each item. There is a steaming method, in which auxiliary ingredients are added, mixed, heated by heating in a suitable container, or steamed until a certain degree. The formulation method is further subdivided according to the intensity of fire and the complementary material used. Cheongcho (법) method is a method of roasting medicinal herbs until it reaches the level prescribed by culture (文火: fire with weak flame) or atomization (武火: fire with strong flame). The runner method is a method of frying medicinal herbs using about 15% alcohol, and the brewing method is a method of steaming medicinal substances using about 15% alcohol. Even if it is the same medicinal plant, raw and ripe materials may not have the same properties or may differ in action. In addition, the complex prescription of a combination of raw materials and raw materials can maximize the therapeutic effect by increasing the efficacy of each drug or canceling toxicity.

In the present specification, the term “preparation treatment” refers to pre-treatment of green tea or ginseng by the “preparation” defined above, and specifically, the increase method is used among the formulation methods, but is not limited thereto, and the formulation method is limited. Can be used without. Specifically, in the present specification, "processing of foaming" refers to a processing step including a process of applying green tea or ginseng a) high temperature and high pressure; And b) may be prepared by a process comprising the step of drying the processed green tea or ginseng, but is not limited thereto, and can be used as long as the formulation is generally used in the art.

In the present specification, "ginseng" can be used without limitation for its type and years, and either dried or undried ginseng can be used. In addition, in the present specification, the ginseng can be used without limitation on the site, and specifically, root can be used.

In the present specification, "green tea" can also be used without limitation on the type and number of days of cultivation, and both dried and dried green tea can be used. In addition, in the present specification, the ginseng can be used without limitation on the site, and specifically, green tea leaves can be used.

As used herein, "anti-aging" is meant to include the production and suppression of pimples, blotch or freckles, the suppression or remission of wrinkles, and includes all phenomena that can slow or alleviate changes over time in an organism. .

The composition, which is one aspect of the present invention, includes a green tea extract that has been 'foamed' and a ginseng extract that has been 'fogged', and has a significantly better anti-aging effect than a mixture of a common green tea extract and a ginseng extract.

In one aspect of the composition of the present invention, the composition can suppress skin wrinkle formation or improve skin wrinkles. The composition of the present invention can inhibit or improve the production of skin wrinkles by promoting the production of pro collagen and inhibiting the expression of collagenase.

In a composition that is one aspect of the present invention, the composition can inhibit collagenase expression.

The composition of the present invention may also promote the production of pro-collagen and inhibit the expression of collagenase, thereby inhibiting or improving the production of skin wrinkles.

In one aspect of the composition of the present invention, the composition includes a moisturizing skin comprising a mixture of extracts of ginseng-treated ginseng extracts and extracts of ginseng-treated green tea as an active ingredient. The composition of the present invention can promote the expression of filaggrin in keratinocytes to increase the moisture content of the skin, and maintain the moisturizing effect of the skin by allowing the skin to retain moisture even after a long time after treatment of the composition.

The composition, which is one aspect of the present invention, has a remarkably better moisturizing effect than a mixture of a general green tea extract and a ginseng extract by synergistic effect of these, including a 'foam-treated' green tea extract and a 'foe-treated' ginseng extract.

In addition, the composition of one aspect of the present invention can strengthen the skin barrier function and induce differentiation of keratinocytes. Therefore, it can be usefully used as a composition for external application for skin to prevent or improve skin dryness, atopic dermatitis, contact dermatitis, or psoriasis caused by incomplete skin differentiation.

In one aspect of the composition of the present invention, the agent-treated ginseng extract may include ginseng polysaccharide.

In one aspect of the composition of the present invention, the ginseng polysaccharide may be 100 years old polysaccharide or black ginseng polysaccharide.

In the present specification, "ginseng polysaccharide" is a pectin analog having a molecular weight of 34,600, and galacturonic acid accounts for about 60%, and other arabinose, rhamnose, glucose, and galactose constitute side chains. .

In one aspect of the composition of the present invention, the green tea extract treated with the foaming agent may include green tea polysaccharide.

As used herein, "baeknyeonsam" means ginseng dried after immersing ginseng at a high temperature, for example, ginseng is dried after being immersed in water at 70 to 90 ° C for 20 to 28 hours, more specifically for 1 day. It is not limited.

In the present specification, "black ginseng" means ginseng that repeats the process of drying ginseng under high pressure and then drying it, for example, putting ginseng in a high pressure steamer, high-pressure steaming at a temperature of 90 to 100 ° C for 3 to 4 hours, and then hot air dryer. In the process of drying at a temperature of 60 to 70 ℃ 8 to 10 times, preferably including ginseng repeated 9 times, but is not limited thereto.

As used herein, "green tea polysaccharide" is derived from green tea and is a group of acidic polysaccharides, unlike polysaccharides found in other plants, produced by combining sugar components and amino acids produced through photosynthesis.

The polysaccharide of the present invention can be prepared by methods known in the art, and the method is not particularly limited. Specifically, after extracting ginseng or green tea leaves at 38-42 ° C., concentrating and removing impurities with a filter, ethanol is added thereto in a dropwise manner and dried by hot air to obtain ginseng or green tea polysaccharide.

In the present specification, 100 years ginseng or black ginseng or green tea polysaccharide is dried by high-pressure steaming of 100 years old ginseng or black ginseng or green tea leaves, concentrated after extracting the mixture, removing impurities with a filter, and adding ethanol in a dropwise manner and drying. Can be obtained through For example, put 100 years old ginseng or black ginseng or green tea leaves in a high-pressure steamer and steam at high pressure at a temperature of 95 ~ 100 ℃ for about 3-4 hours. After drying to a temperature of ~ 70 ° C, 1 kg of processed ginseng and green tea mixture using a foaming agent is pulverized with a blender, and then 8 to 11 ℓ of purified water is added, stirred and extracted with hot water at 38 to 42 ° C for 24 hours, and then 10 to It was immersed at 20 ° C for 20 to 28 hours. Thereafter, the residue and the filtrate are separated through filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. This includes, but is not limited to, a method of adding concentrated amounts of 3 to 5 times ethanol in a dropwise manner and drying by hot air.

In one aspect of the present invention, in the composition, the weight ratio between the extract of the ginseng-treated ginseng and the extract of the gadded-treated green tea may be 0.1 to 10: 1. The synergistic effect of the two components can be maximized at the above weight ratio. In view of the above, the weight ratio between the extract of the foamed ginseng extract and the extract of the foamed green tea may be 0.5 to 9.5: 1, 1 to 9: 1, or 1.5 to 8.5: 1.

In one aspect of the present invention, in the composition, the composition may include an extract of a ginseng-treated ginseng and an extract of a green tea-treated ginseng, respectively, in an amount of 0.001 to 10 wt% based on the total weight of the composition. This is because if the content of the extract is less than 0.001% by weight, the skin wrinkle improvement and moisturizing effect is negligible, and when it exceeds 10% by weight, there is no obvious increase in the effect of increasing the content. In view of the above, the composition of the present invention, based on the total weight of the composition, the composition is 0.005 ~ 9.5% by weight, 0.01 ~ 9% by weight, 0.03 ~ 8.5% by weight of the extract of the ginseng-extracted and ginseng-treated green tea, respectively %, 0.05 to 8 wt%, 0.07 to 7.5 wt%, 0.09 to 7 wt%, 0.1 to 6.5 wt%, 0.3 to 6 wt%, 0.5 to 5.5 wt% or 0.7 to 5 wt%.

In one aspect of the composition of the present invention, the composition includes a cosmetic composition.

The cosmetic composition according to the present invention can be provided in any formulation suitable for topical application. For example, it may be provided in the form of a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in an oil phase, suspension, solid, gel, powder, paste, foam, or aerosol composition. Compositions of these formulations can be prepared according to conventional methods in the art.

The cosmetic composition according to the present invention may contain other ingredients that can give a synergistic effect to the main effect, within the range not impairing the main effect, in addition to the above-mentioned substances. In addition, the cosmetic composition according to the present invention may further include a moisturizer, emollient, ultraviolet absorber, preservative, disinfectant, antioxidant, pH adjusting agent, organic and inorganic pigments, fragrances, coolants or limiting agents. The compounding amount of the components can be easily selected by those skilled in the art within a range that does not impair the objects and effects of the present invention, and the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, based on the total weight of the composition. have.

In one aspect of the composition of the present invention, the composition includes a pharmaceutical composition.

When the composition according to the present invention is applied to a pharmaceutical product, a commercially available inorganic or organic carrier may be added as an active ingredient to form a solid, semi-solid, or liquid form as an oral administration or parenteral administration.

Examples of the preparation for oral administration include tablets, pills, granules, capsules, powders, granules, powders, emulsions, syrups, pellets and the like. In addition, preparations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated by carrying out according to the commercial method, and surfactants, excipients, colorants, spices, preservatives, stabilizers, buffers, suspending agents, and other commonly used auxiliary agents can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous, and the like.

The active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, gender, weight, pathology and severity of the subject to be administered, the route of administration or the judgment of the prescriber. Determination of the application amount based on these factors is within the level of those skilled in the art, the daily dose of which is, for example, 0.1 mg / kg / day to 100 mg / kg / day, more specifically 5 mg / kg / day to 50 mg / kg / Can be a day, but is not limited to this.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

[Comparative Example 1] Ginseng polysaccharide production

After crushing 1 kg of dried white ginseng with a blender, 10 l of purified water was added, stirred extracted with hot water at 40 ° C. for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate are separated through filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Here, ethanol of 4 times the concentrated amount was added in a dropwise manner and dried by hot air to obtain 65 g of ginseng polysaccharide.

[Comparative Example 2] Green tea polysaccharide production

After crushing 1 kg of the first processed (steaming process) green tea leaves with a blender, 10 l of purified water was added, stirred and extracted with hot water at 40 ° C for 24 hours, and then deposited at 15 ° C for 1 day. Thereafter, the residue and the filtrate are separated through filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Here, ethanol of 4 times the concentrated amount was added in a dropwise manner and dried by hot air to obtain 72 g of green tea polysaccharide.

[Comparative Example 3] Preparation of ginseng foam extract containing ginseng polysaccharide (red ginseng polysaccharide)

After putting the undried ginseng into a high-pressure steamer and steaming it under high pressure at a temperature of 97 ° C for about 4 hours, and then drying the steamed ginseng that has completed the above process steps at a temperature of 65 ° C in a hot air dryer, and then processing it using a foaming agent. After crushing 1 kg of ginseng with a blender, 10 l of purified water was added, stirred extracted with hot water at 40 ° C. for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate are separated through filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Here, 70 g of the processed ginseng polysaccharide (red ginseng polysaccharide) was obtained by adding a concentrated amount of ethanol in a multiple of 4 times in a dropwise manner and drying it by hot air to utilize a foaming agent, an herbal processing technique.

[Comparative Example 4] Preparation of green tea foaming extract containing green tea polysaccharide

The first processed (steaming process) green tea leaves were put in a high-pressure steamer and steamed at a temperature of 97 ° C for about 4 hours, followed by drying the steamed green tea leaves that had completed the above process steps at a temperature of 65 ° C in a hot air dryer. Then, 1 kg of processed green tea leaves were pulverized with a blender using a foaming agent, and then 10 l of purified water was added, stirred and extracted with 40 ° C. hot water for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate are separated through filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Here, 70% of the processed green tea polysaccharide was obtained by adding a concentrated amount of ethanol of 4 times in a dropwise manner and drying it by hot air to utilize a foaming agent, which is a herbal processing technique.

[Comparative Example 5] Preparation of a mixture of ginseng extract and green tea extract

Ginseng polysaccharide and green tea polysaccharide prepared in Comparative Example 1 and Comparative Example 2 were simply mixed in a ratio of 1: 1.

[Example 1-1] Preparation of 100 years old ginseng

1 kg of ginseng was immersed in water at 90 ° C for 1 day, and then dried to prepare 100 year old ginseng.

[ Example  1-2] 100 years  Preparation of a mixture of extract and green tea extract

50% by weight of the 100 years old ginseng prepared in Example 4-1 and 50% by weight of the first processed (steaming process) green tea leaves were put in a high-pressure steamer, followed by high-pressure steaming at a temperature of 97 ° C for about 4 hours, and then completing the above process steps. After drying one steamed ginseng and green tea mixture at a temperature of 65 ° C in a hot air dryer, crushed 1 kg of processed white ginseng and green tea mixture using a foamer with a blender, add 10 ℓ of purified water, and heat to 40 ℃ with hot water 24 After stirring extraction for an hour, it was immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate are separated through filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. The concentrated amount of ethanol of 4 times in a concentrated amount was added in a dropwise manner and dried by hot air to obtain 770 g of a mixture steamed white polysaccharide and green tea polysaccharide mixture.

[Example 2-1] Preparation of black ginseng

Black ginseng, a ginseng, was prepared by putting 1 kg of ginseng into a high-pressure steamer, steaming it at a temperature of 97 ° C. for 4 hours, and then repeating the process of drying at 65 ° C. in a hot air dryer 9 times.

[Example 2-2] Preparation of mixture of black ginseng extract and green tea extract

50% by weight of black ginseng prepared in Example 5-1 and 50% by weight of primary processed (steaming process) green tea leaves were put in a high-pressure steamer, followed by high-pressure steaming at a temperature of 97 ° C for about 4 hours, and then completing the above process steps. After drying the mixture of steamed ginseng and green tea at a temperature of 65 ° C in a hot air dryer, crushed 1 kg of the processed black ginseng and green tea mixture using a foamer with a blender, add 10 l of purified water, and stir for 24 hours with 40 ° C hot water After extraction, it was immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate are separated through filtration and centrifugation, and the separated filtrate is concentrated under reduced pressure to 1/10 of the amount of purified water. Here, ethanol of 4 times the concentrated amount was added in a dropwise manner and dried by hot air to obtain 70 g of a mixture of steamed black ginseng polysaccharide and green tea polysaccharide.

[Test Example 1] Measurement of collagenase expression inhibitory efficacy

The inhibitory ability of collagenase production in Example 1 was measured in comparison with control groups, tocopherol and EGCG.

The test was performed when human fibroblasts were put into 5,000 cells / well in a 96-well plate containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal calf serum, and grew to about 90%. Incubated until. After culturing for 24 hours in serum-free DMEM medium, 10 ^-4 processed ginseng polysaccharide and green tea polysaccharide mixtures were processed into serum-free DMEM medium using the herbal preparation technique of Comparative Examples 1-5 and Examples 1-2. After treatment at a molar concentration for 24 hours, a cell culture solution was collected.

The level of collagenase production was measured using a commercially available collagenase measuring instrument (Amersham Pharma Co., Ltd.). First, the cultured cell culture medium was added to a 96-well plate uniformly coated with primary collagenase antibody, and the antigen-antibody reaction was performed in a thermostat for 3 hours.

After 3 hours, the chromophore-bound secondary collagen antibody was added to a 96-well plate and reacted again for 15 minutes. After 15 minutes, a color-producing substance was added to induce color development at room temperature for 15 minutes, and when 1M sulfuric acid was added again to stop the reaction (color development), the color of the reaction solution was yellow, and the degree of yellow was different depending on the progress of the reaction.

The absorbance of the yellow-colored 96-well plate was measured at 405 nm using an absorbometer, and the degree of synthesis of collagenase was calculated by the following equation (1). At this time, the reaction absorbance of the cultured cell culture medium of the group not treated with the composition was used as a control.

[Equation 1]

Collagenase expression level (%) = 100- {(absorbance of the cell group treated with the comparative example or example ÷ absorbance of the control group) X 100}

The results of measuring the inhibitory efficacy of collagenase expression of the test substances in the human fibroblasts are shown in Table 1 below, and this is compared with the collagenase expression level of the untreated group as 100. .

matter Collagenase
Expression level (%) Untreated group 100 Tocopherol (positive control) 76 EGCG (positive control group) 63 Comparative Example 1 79 Comparative Example 2 77 Comparative Example 3 77 Comparative Example 4 74 Comparative Example 5 77 Example 1 68 Example 2 65

As a result, it was confirmed that the mixture of the ginseng foam extract and the green tea foam extract according to the present invention inhibits collagenase expression in vitro.

In addition, as shown in Table 1, it was confirmed that Examples 1 to 2 containing the respective foam extracts had better collagenase inhibitory effect than each of the comparative examples, and in particular, collagenase superior to Comparative Example 5 It was confirmed that it has an inhibitory effect. In addition, it was confirmed that Examples 1 to 2 containing each foam extract had a higher collagenase expression inhibitory effect than tocopherol, a positive control.

[Test Example 2] Procollagen production promoting efficacy experiment

The ability to generate procollagen in Comparative Examples and Examples was measured by comparing with vitamin C.

When the test was performed, human fibroblasts were put into 5,000 cells / well in a 96-well plate containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal calf serum. Incubated until. After culturing in serum-free DMEM medium for 24 hours, ginseng polysaccharide, green tea polysaccharide mixture and vitamins processed using the herbal preparation technique of Comparative Examples 1 to 5 and Examples 1 to 2 dissolved in serum-free DMEM medium After treating C at a concentration of 10 ^-4 for 24 hours, a cell culture solution was collected. After 24 hours, the amount of procollagen released in the medium was measured using a procollagen type-1 C-peptide EIA kit (MK101, Takara, Japan).

The degree of procollagen production in the non-treated group was set to 100, and the degree of procollagen production in the group treated with the composition was prepared, and the results are shown in Table 2.

matter Procollagen production degree (%) Untreated group 100 Vitamin C 121 Comparative Example 1 110 Comparative Example 2 106 Comparative Example 3 111 Comparative Example 4 108 Comparative Example 5 108 Example 1 119 Example 2 121

As a result, the higher the degree of procollagen production, the higher the degree of collagen production, and thus the production of skin wrinkles could be prevented.

In addition, Examples 1 to 5 according to the present invention were confirmed to promote the production of procollagen in vitro (in vitro), and it can be confirmed that these effects are as good as vitamin C, which is known as an essential component for collagen synthesis. there was. In particular, it was confirmed that Examples 1 to 2 according to the present invention had a significantly higher degree of procollagen production promoting effect compared to Comparative Examples 1 to 5.

[Test Example 3] Induction test for differentiation of human keratinocytes

In order to confirm the skin barrier function and skin moisturizing ability of the comparative examples and examples, a test using absorbance was performed.

First keratinocytes of primary cultured humans (Purchase destination: Dr. NE Fusenig, Deutsches Krebsforschungszentrum, Heidelberg, Germany) was added to the culture flask and attached to the bottom, and then added to the culture solution composed as shown in Table 3 below, so that the cells were approximately 70-80% of the floor area. Incubated for 5 days until growing. After harvesting the cells and washing with phosphate buffered saline (PBS), 10 mM concentration of Tris-HCl (Tris-HCl) containing 2% SDS (Sodium Dodecyl Sulfate) and 20 mM concentration of DTT (Dithiothreitol). , pH 7.4) 1 ml was added and sonication was performed for 3 minutes, followed by boiling for 10 minutes. This was centrifuged at 1200 rpm for 30 minutes, and the separated precipitate was suspended again in 1 ml of PBS to measure absorbance at 340 nm. Separately, a portion of the solution after sonication was taken to measure the protein content, and was used as a reference when evaluating the degree of cell differentiation. Low calcium (0.03mM) treatment group and high calcium (1.2 mM) treatment group were used as negative / positive control, respectively, and the low calcium concentration was added to each of Comparative Examples 1 to 5 and Examples 1 to 2 for each concentration. The results are shown in Table 3 below.

Concentration Differentiation capacity in keratinocytes (%) Control Low Ca ²⁺ (0.03 mM) 100 High Ca ²⁺ (1.2 mM) 215 Comparative Example 1 0.05ppm 106 0.1ppm 109 Comparative Example 2 0.05ppm 101 0.1ppm 102 Comparative Example 3 0.05ppm 108 0.1ppm 112 Comparative Example 4 0.05ppm 102 0.1ppm 105 Comparative Example 5 0.05ppm 104 0.1ppm 106 Example 1 0.05ppm 112 0.1ppm 121 0.5 ppm 127 Example 2 0.05 ppm 113 0.1 ppm 125 0.5 ppm 129

As a result, comparing the effect of promoting cell differentiation by measuring the amount of CE (Cornified Envelop) generated during keratinocyte differentiation, it was confirmed that the mixtures of the foam extracts of Examples 1 to 2 promote differentiation in keratinocytes. . In addition, it was confirmed that the mixture of the foam extract of each of the Examples of the present invention has a significantly higher differentiation ability of keratinocytes than the comparative example containing the general extract. Therefore, it was confirmed that the composition of the present invention has better skin barrier function and skin moisture protection.

[Test Example 4] Measurement of the expression of filaggrin through RT-PCR analysis

In order to test the skin moisturizing ability of each of the comparative examples and examples, the degree of pilar green expression was measured.

The cell line used for the experiment was Dr. of the German Cancer Research Center. As a human kerationocyte HaCaT cell line received from Fusenig, the cells were divided into 1 ㅧ 10 ⁵ cells in a 60 mm dish and cultured for 1 day, and then the test material was treated as shown in Table 4 below and then cultured for 24 hours. . From the cells cultured and tested by the above method, total RNA was extracted using Trizol (Trizol, Gibco Laboratories, USA), and then provided in a method provided by One Step RNA PCR Kit (AMV) (Takara Bio Inc., Japan). In accordance, RT-PCR (reverse transcriptional polymerase chain reaction) analysis was performed. At this time, a primer (Bionia, Korea) was used as a primer (filaggrin), and beta-actin was used as an internal control to compare expression levels. . After the image analysis, the control value was set to 100 and the comparison values are shown in Table 4 below.

Concentration Filla green production (%) Untreated control - 100 Comparative Example 1 0.05ppm 110 0.1ppm 116 Comparative Example 2 0.05ppm 115 0.1ppm 126 Comparative Example 3 0.05ppm 108 0.1ppm 115 Comparative Example 4 0.05ppm 115 0.1ppm 128 Comparative Example 5 0.05ppm 113 0.1ppm 120 Example 1 0.05ppm 122 0.1ppm 159 0.5 ppm 201 Example 2 0.05 ppm 123 0.1 ppm 160 0.5 ppm 200

As shown in Table 4, it was confirmed that Examples 1 to 2 of the present invention promoted the expression of pilargreen in keratinocytes. In particular, it was confirmed that Examples 1 to 2 of the present invention significantly promoted the expression of pilar green compared to Comparative Examples 1 to 5. Therefore, it was confirmed that the composition of the present invention has an effect of promoting the production of natural moisturizing factors of the skin and enhancing the skin moisturizing ability.

[Test Example 5] Measurement of skin moisturizing improvement effect

According to the composition ratios of Tables 5 and 6, formulation examples 1 to 2 (Table 5) and comparative formulations 1 to 5 (Table 6), which are external preparations for nutritional cream formulations, were prepared. The unit of content ratio of the following blending components is% by weight.

Ingredients Formulation Example 1 Formulation Example 2 Purified water To 100 To 100 Example 1 0.1 - Example 2 - 0.1 Hydrogenated vegetable oil 1.50 1.50 Stearic acid 0.60 0.60 Glycerol stearate 1.00 1.00 Stearyl alcohol 2.00 2.00 Polyglyceryl-10 pentastearate + behenyl alcohol +
Sodium Stearoyl Lactate 1.00 1.00 Arachidyl behenyl alcohol + arachidylglucoside 1.00 1.00 Cetylaryl alcohol + Cetaryl glucoside 2.00 2.00 PEG-100 stearate + glycerol oleate + propylene glycol 1.50 1.50 Caprylic / caric triglyceride 11.00 11.00 Cyclomedicon 6.00 6.00 Preservatives, incense Proper Proper Triethanol amine 0.1 0.1

Ingredients Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Comparative Formulation Example 4 Comparative Formulation Example 5 Purified water To 100 To 100 To 100 To 100 To 100 Comparative Example 1 0.1 - - - - Comparative Example 2 - 0.1 - - - Comparative Example 3 - - 0.1 - - Comparative Example 4 - - - 0.1 - Comparative Example 5 - - - - 0.1 Hydrogenated vegetable oil 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10 pentastearate + behenyl alcohol + sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol + arachidylglucoside 1.00 1.00 1.00 1.00 1.00 Cetylaryl alcohol +
Cetearyl glucoside 2.00 2.00 2.00 2.00 2.00 PEG-100 stearate + glycerol oleate + propylene glycol 1.50 1.50 1.50 1.50 1.50 Caprylic / caric triglyceride 11.00 11.00 11.00 11.00 11.00 Cyclomedicon 6.00 6.00 6.00 6.00 6.00 Preservatives, incense Proper Proper Proper Proper Proper Triethanol amine 0.1 0.1 0.1 0.1 0.1

The following experiments were performed using the formulation examples and comparative formulation examples of Tables 5 and 6 in order to confirm the skin moisturizing effect on the humans of Examples 1 to 2. Divide 70 healthy women in their 30s into 7 sets of 10, and apply the nutrient creams of Formulation Examples 1 and 2 and Comparative Formulation Examples 1 to 5 to the face twice a day for 4 weeks twice a day at a temperature of 24-26 ° C and 75% humidity. Temperature, constant humidity, and humidity conditions (24 ° C, 40% relative humidity) after the start of application, 1 week, 2 weeks, 4 weeks after application, and 2 weeks after the application is stopped (6 weeks in total) The moisture content of the skin was measured with a connometer. The increment of the measured value at each measurement is expressed as a percentage based on the measured value of the coniometer immediately before the start of the test, and the results are shown in Table 7 below.

Clinical results Moisture growth rate (%) 1 week 2 weeks elapsed 4 weeks elapsed 6 weeks elapsed Formulation Example 1 32 39 40 30 Formulation Example 2 32 38 39 30 Comparative Formulation Example 1 30 32 32 18 Comparative Formulation Example 2 30 33 34 21 Comparative Formulation Example 3 32 34 34 23 Comparative Formulation Example 4 31 33 33 22 Comparative Formulation Example 5 31 33 33 22

As shown in Table 7 above, it was found that Formulation Examples 1 to 2 containing the extracts of the present invention significantly increased skin moisture retention than the groups to which Comparative Formulation Examples 1 to 5 were applied. In addition, the value after 6 weeks, which is 2 weeks after the application of the test substance is stopped, appears similar to the value after 1 to 2 weeks after the test substance was applied, and it is possible to apply the formulation examples 1 to 2 even without applying the test substance. By this, it was confirmed that the water retention amount of the skin was continuously maintained for a certain period. Therefore, it was found that the composition of the present invention enhances the skin moisturizing ability.

Hereinafter, an example of the formulation of the composition according to the present invention will be described, but the pharmaceutical composition and the cosmetic composition may be applied in various dosage forms, which are not intended to limit the present invention, but only to be specifically described.

[Formulation Example 1] Soft capsule

150 mg of a ginseng extract treated with a formulation, 150 mg of a green tea extract with a formulation, 2 mg of palm oil, 8 mg of palm hardened oil, 4 mg of lead, and 6 mg of lecithin were mixed and filled with 400 mg per capsule according to a conventional method. It was prepared.

[Formulation Example 2] Tablet

After mixing granulated ginseng extract 150 mg, dried ginseng extract 150 mg, glucose 100 mg, red ginseng extract 50 mg, starch 96 mg and magnesium stearate 4 mg, and adding 40 mg of 30% ethanol to form granules, 60 It was dried at ℃ and compressed into tablets using a tablet press.

[Formulation Example 3] Granules

150 mg of ginseng extract treated with inclusions, 150 mg of green tea extract with inclusions, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch are mixed, 100 mg of 30% ethanol is added to form granules, and dried at 60 ° C to obtain granules. After formation, the cells were filled. The final weight of the contents was 1 g.

[Formulation Example 1] Flexible cosmetic (skin lotion)

According to the composition shown in Table 8, a softening agent was prepared by a conventional method.

Ingredients Content (% by weight) Extract of ginseng and green tea treated with foam (Ginseng: green tea = 1: 1) 0.2 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 PG-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservatives, pigments, flavors Proper Purified water Balance

[Formulation Example 2] Nutritional Cosmetics (Milk Lotion)

According to the composition shown in Table 9, nutrient makeup was prepared by a conventional method.

Ingredients Content (% by weight) Extract of ginseng and green tea treated with foam (Ginseng: green tea = 1: 1) 1.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / Capric Triglyceride 5.0 Squalane 5.0 Solvitasesquioleate 1.5 Floating paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservatives, pigments, flavors Proper Purified water Balance

[Formulation Example 3] Nutrition Cream

A nutritional cream was prepared in a conventional manner according to the composition shown in Table 10 below.

Ingredients Content (% by weight) Extract of ginseng and green tea treated with foam (Ginseng: green tea = 1: 1) 2.0 glycerin 3.0 Butylene glycol 3.0 Floating paraffin 7.0 Beta glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglyceride 3.0 Squalane 5.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservatives, pigments, flavors Proper Purified water Balance

[Formulation Example 4] Massage Cream

Massage creams were prepared in a conventional manner according to the composition shown in Table 11 below.

Ingredients Content (% by weight) Extract of ginseng and green tea treated with foam (Ginseng: green tea = 1: 1) 3.0 glycerin 8.0 Butylene glycol 4.0 Floating paraffin 45.0 Beta glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglyceride 3.0 Wax 4.0 Cetearyl Glucoside 1.5 Sesqui oleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservatives, pigments, flavors Proper Purified water Balance

[Formulation Example 5] Pack

Packs were prepared by conventional methods according to the compositions listed in Table 12 below.

Ingredients Content (% by weight) Extract of ginseng and green tea treated with foam (Ginseng: green tea = 1: 1) 0.2 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 suitable Preservatives, pigments, flavors Proper Purified water Balance

[Formulation Example 6] Ointment

Ointments were prepared in a conventional manner according to the composition described in Table 13 below.

Ingredients Content (% by weight) Extract of ginseng and green tea treated with foam (Ginseng: green tea = 1: 1) 0.2 glycerin 8.0 Butylene glycol 4.0 Floating paraffin 15.0 Beta glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglyceride 3.0 Squalane 1.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservatives, pigments, flavors Proper Purified water Balance

Since the specific parts of the present invention have been described in detail above, it is obvious that for those skilled in the art, this specific technique is only a preferred embodiment, and the scope of the present invention is not limited thereby. something to do. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (9)

Contains a mixture of a ginseng extract and a green tea extract treated with foamed ginseng polysaccharide or black ginseng polysaccharide,
The foaming treatment is by using a steaming method,
A composition that is a cosmetic composition. Contains a mixture of a ginseng extract and a green tea extract treated with foamed ginseng polysaccharide or black ginseng polysaccharide,
The foaming treatment is by using a steaming method,
A composition that is a pharmaceutical composition for inhibiting skin wrinkle formation or improving skin wrinkles According to claim 1,
The composition is a composition for inhibiting skin wrinkle formation or improving skin wrinkles. The method of claim 2 or 3,
The composition is a composition that inhibits collagenase expression. According to claim 1,
The composition is for moisturizing the skin comprising a mixture of a ginseng extract and a green tea extract treated with a formulation as an active ingredient. The method according to claim 1 or 2,
The weight ratio between the agent-treated ginseng extract and the agent-treated green tea extract is 0.1 to 10: 1, composition. The composition of claim 1 or 2, wherein the composition comprises 0.001 to 10% by weight, respectively, based on the total weight of the composition, the ginseng extract and the green tea extract treated with the agent. delete delete