KR102105820B1 - Composition for UV protection containing polysaccharides extracted from processed Panax ginseng and Green tea - Google Patents

Abstract

The present invention relates to a composition for whitening and UV protection containing ginseng polysaccharide and green tea polysaccharide, and more specifically, by mixing and mixing each polysaccharide extracted from the extract of ginseng and green tea treated with a foaming agent, a whitening effect and an ultraviolet blocking effect And it relates to a composition excellent in preventing skin damage caused by ultraviolet rays.

Description

Composition for UV protection containing polysaccharides extracted from processed Panax ginseng and Green tea}

The present invention relates to a composition for whitening and UV protection containing ginseng polysaccharide and green tea polysaccharide, and more specifically, by mixing and mixing each polysaccharide extracted from the extract of ginseng and green tea treated with a foaming agent, a whitening effect and an ultraviolet blocking effect And it relates to a composition excellent in preventing skin damage caused by ultraviolet rays.

Human skin color is determined by a number of factors, such as the activity of melanocytes that produce melanin pigments, the distribution of blood vessels, the thickness of the skin, and the presence or absence of pigments inside and outside the human body, including melanocytes and melanosite to tyre. The most important is a black pigment called melanin, which is produced by the action of several enzymes, such as kinase. The formation of melanin pigments is influenced by genetic factors, physiological factors related to hormone secretion and stress, and environmental factors such as ultraviolet irradiation. Melanin is present in the skin and has an important function of protecting the body from ultraviolet rays, but it is known that melanin is excessively produced, thereby forming spots or freckles, and also promoting skin aging and inducing skin cancer.

Ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, or derivatives thereof, in order to treat or alleviate excessive melanin pigmentation caused by exposure to ultraviolet rays and skin pigmentation abnormalities such as spots, freckles or pigmentation , Tyrosinase inhibitory substances have been used in cosmetics or pharmaceuticals, and their use is limited due to insufficient whitening effects, safety problems for skin, and formulation and stability problems when formulated in cosmetics.

Ultraviolet rays contained in sunlight promotes erythema formation or melanin production in skin cells when excessively irradiated to the skin, which may cause the occurrence of blemishes and blemishes, and react with the sebum secreted in the epidermis to create skin problems. Not only does it cause, but in severe cases it can also cause skin cancer. UV rays are divided into UV-A (320-400 nm), UV-B (280-320 nm) and UV-C (200-280 nm) depending on their wavelength. Since ultraviolet rays are mostly absorbed in the atmosphere, ultraviolet rays that directly affect the human body are known as UV-A and UV-B.

The sunscreen used for the purpose of preventing skin damage caused by ultraviolet rays refers to a preparation that exhibits a sunscreen effect by applying a special substance to a certain area of the skin. The sunscreen effect refers to a unit called sun protection factor (SPF). Use to indicate the degree of blocking of the product. The sunscreen index is calculated by the formula of the FDA (Food and Drug Administration), and the minimum amount of UV-B (Minimal Erythema Dose; MED) without sunscreen and UV-B after sunscreen is applied It shows the ratio of the minimum erythema. In other words, as a multiple of the degree to which natural skin blocks UV rays, the higher the value of the UV ray index, the better the effect of sun protection, but the more comfortable it is to the skin and the higher the side effects.

Sunscreens are broadly classified into chemical sunscreens and physical sunscreens. Chemical sunscreens that use chemical absorption of ultraviolet light as a mechanism are well known organic blocking agents such as cinnamic acid, salicylic acid, and benzophenone, and physical sunscreens that use physical scattering and shielding of ultraviolet light as a mechanism are titanium dioxide, zinc oxide, etc. The same inorganic blockers are well known. In the case of a chemical sunscreen, it has an excellent sunscreen effect, but has a disadvantage in that the applied wavelength range is narrow, sticky and shiny, and can be absorbed into the skin in a molecular form, causing skin irritation. In the case of a physical sunscreen, the amount of transmitted ultraviolet rays is reduced by reflecting and scattering ultraviolet rays. In general, an inorganic compound in the form of ultrafine particles is used, and this material has a very high photorefractive index, and has excellent heat resistance compared to an organic material sunscreen. Skin irritation is relatively low compared to chemical sunscreen, but it is not cosmetically appropriate due to its disadvantages of causing heavy use and whitening (Public Patent No. 2003-0046103).

Various studies have been conducted on sunscreens to compensate for the disadvantages of chemical sunscreens and physical sunscreens. Specifically, a form in which a chemical sunscreen and a physical sunscreen are mixed in an appropriate ratio is a method that is currently mostly applied to sunscreen products, but the above-mentioned fundamental disadvantages exist. In addition, the form of adsorbing titanium dioxide, an inorganic blocking agent on the surface of inorganic or polymer particles, or containing an organic blocking agent inside the particle was supplemented in terms of skin safety, but has a disadvantage that the manufacturing process is complicated and the UV blocking efficiency is not high. Lastly, there are some types of inorganic materials such as titanium dioxide and zinc oxide that are manufactured as ultra-fine particles to compensate for the disadvantages of existing inorganic barrier agents and have a high-efficiency blocking effect, but this cannot completely reduce the whitening phenomenon, and the smaller the particle size, the more comfortable it is. This has the disadvantage of getting worse. Organic and inorganic sunscreens based on such chemical substances have become interested in natural sunscreens which are completely harmless to humans because their side effects are a problem.

As an example of the prior invention regarding natural sunscreens, natural hydroxyanthracene polyglycosides have been reported as sunscreens (Bader et al., Cosmetics & Toiletries, 96 (10), pp67, 1981), and the significance of coffee as sunscreen. Usage has been reported in French Patent No. 2-479-688. Similarly, natural extracts of aloe, francula, senna and cascara containing anthraquinone derivatives have also been reported as useful sunscreens (Fox et al., Cosmetics & Toiletries, 102 (3), pp41, 1987).

Thus, the present inventors can treat ginseng and green tea in a foamed form, and extract polysaccharides therefrom, thereby obtaining polysaccharides in a higher yield than ginseng or green tea that is not foamed, and use the ginseng polysaccharides and green tea polysaccharides obtained at the same time. When the synergistic effect is provided, the skin whitening effect and the ultraviolet ray blocking effect are better than those of each of these polysaccharides, and the irritation to the skin is small, and the present invention has been completed.

Accordingly, it is an object of the present invention to provide a composition having excellent skin whitening effect and sunscreen effect by simultaneously using ginseng polysaccharide extracted from ginseng and green tea extract treated with a foamed agent and green tea polysaccharide.

In order to achieve the above object, the present invention provides a composition for whitening and UV protection containing ginseng polysaccharide and green tea polysaccharide extracted from extracts of ginseng and green tea that have been treated as active ingredients.

The composition of the present invention is capable of obtaining polysaccharides with high yield by extracting polysaccharides from the extracts of ginseng and green tea treated with foam, and also provides excellent skin whitening effect by simultaneously containing ginseng polysaccharides and green tea polysaccharides, and skin by ultraviolet rays. It has the effect of preventing damage. Therefore, the composition containing the ginseng polysaccharide and green tea polysaccharide reduces pigmentation symptoms such as blemishes and freckles, makes the skin color appear uniform and bright, has a very good UV protection effect, and has no irritation to the skin, so it is an external preparation for skin. It may be useful as a composition or pharmaceutical composition.

The composition of the present invention contains ginseng polysaccharides and green tea polysaccharides as active ingredients, and the ginseng polysaccharides and green tea polysaccharides are extracted from processed ginseng and green tea using a herbal processing technology, a foaming agent.

Hereinafter, the present invention will be described in more detail.

Ginseng (Panax ginseng CA Meyer) polysaccharide used in the present invention is a pectin analog having a molecular weight of 34,600, and galacturonic acid occupies about 60%, and other arabinose, rhamnose, glucose, and Galactose and the like constitute a side chain. Ginseng used in the present invention is a plant belonging to the genus Agapiaceae, and in the present invention, ginseng can be used without limitation to its type and lotus root, and both dried and dried ginseng can be used. In addition, in the present invention, ginseng can be used without limitation on the site, and specifically, the root of ginseng can be used.

In addition, the green tea polysaccharide used in the present invention is derived from green tea, and is an acidic polysaccharide group unlike polysaccharides found in other plants, and is produced by combining sugar components and amino acids produced through photosynthesis. In the present invention, green tea can be used without limitation on its type and number of cultivation days, and both dried and undried green tea can be used. In addition, in the present invention, green tea can be used without limitation on the site, and specifically, leaves of green tea can be used.

The agent of the present invention refers to a pharmaceutical technology that changes the original properties of a medicine by processing the medicine based on the herbal theory. The agent may include boiling, steaming, roasting, roasting, or heating to reduce the toxicity of the drug, to preserve the drug, to improve the drug, and to improve the convenience of taking the drug. In addition, the preparation method includes, for example, a second method of frying the medicinal material, a method in which the auxiliary material is impregnated into the drug tissue by roasting the medicinal material together with a certain amount of the liquid auxiliary material, and a liquid in accordance with the regulation of the preparation of each item of medicinal material There is a steaming method, in which auxiliary ingredients are added and mixed, heated by heating in a suitable container, or steamed until a certain degree.

In addition, the preparation method is further subdivided according to the intensity of fire and the complementary material used. Cheongcho (법) method is a method of roasting medicinal herbs until it reaches the level prescribed by culture (文火: fire with weak flame) or atomization (武火: fire with strong flame). The runner method is a method of frying medicinal materials using about 15% alcohol, and the brewing method is a method of steaming medicinal materials using about 15% alcohol. Even if it is the same medicinal plant, raw and unripe materials may not have the same properties or differ in action. In addition, the complex prescription of a combination of raw materials and raw materials can maximize the therapeutic effect by increasing the efficacy of each drug or canceling toxicity.

The foaming treatment in the present invention means a process of pre-processing ginseng or green tea before preparing the extract by the above-described foaming method, and ginseng to increase the content of polysaccharide used as an active ingredient in the present invention in ginseng or green tea extract Alternatively, green tea is used as a formulation.

Specifically, the green tea polysaccharide and ginseng polysaccharide of the present invention are obtained by preparing ginseng root or green tea leaf extract with water at 38-42 ° C., concentrating it, removing impurities with a filter, and then adding ethanol thereto dropwise and drying by hot air. can do.

The composition of the present invention may contain ginseng polysaccharide and green tea polysaccharide in an amount of 0.001 to 10% by weight, respectively, based on the total weight of the composition, and preferably ginseng polysaccharide and green tea polysaccharide, respectively, 0.005 to 9.5% by weight, based on the total weight of the composition , 0.01 ~ 9 wt%, 0.03 ~ 8.5 wt%, 0.05 ~ 8 wt%, 0.07 ~ 7.5 wt%, 0.09 ~ 7 wt%, 0.1 ~ 6.5 wt%, 0.3 ~ 6 wt%, 0.5 ~ 5.5 wt% or 0.7 It can be contained in an amount of ~ 5% by weight.

In the present invention, ginseng polysaccharide and green tea polysaccharide are used by mixing in a range of 0.1 to 10: 1 by weight. In addition, in order to maximize the synergistic effect due to the combination of the two components, preferably, ginseng polysaccharide and green tea polysaccharide are used in a range of 0.5 to 9.5: 1, 1 to 9: 1 or 1.5 to 8.5: 1 by weight. You can.

The composition according to the present invention can be usefully used for whitening and preventing skin damage caused by ultraviolet rays.

The composition of the present invention may be formulated as a composition for external application for skin, particularly as a cosmetic composition, and may contain a medium or a base that is cosmetically or dermatologically acceptable. In addition, the composition of the present invention may be provided in any formulation suitable for topical application, for example, a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in an oil phase, suspension, solid, gel, powder, It can be provided in the form of a paste, foam or aerosol composition. Compositions of these formulations can be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may include other ingredients that can give a synergistic effect to the main effect, preferably within a range not impairing the main effect other than the above-mentioned substances. In addition, the composition according to the present invention may further include a moisturizer, emollient, ultraviolet absorber, preservative, antiseptic, antioxidant, pH adjuster, organic and inorganic pigments, fragrances, coolants or limiting agents. The compounding amount of the components can be easily selected by those skilled in the art within a range that does not impair the objects and effects of the present invention, and the compounding amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, based on the total weight of the composition. .

In addition, the composition of the present invention can be formulated as a pharmaceutical composition. When the composition according to the present invention is applied to pharmaceuticals, an inorganic or organic carrier compatible with the active ingredient used in the present invention may be added to form a solid, semi-solid, or liquid form as an oral administration or parenteral administration. , The pharmaceutical composition according to the present invention may be administered by oral, parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous, and the like.

Examples of the formulation for oral administration include tablets, pills, granules, capsules, powders, granules, powders, emulsions, syrups, pellets, and the like. In addition, formulations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated into an active ingredient by carrying out according to the commercial method, in which case surfactants, excipients, colorants, spices, preservatives, stabilizers, buffers, suspending agents, and other commonly used adjuvants can be suitably used. have.

The dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight, pathology and severity of the subject to be administered, the route of administration or the judgment of the prescriber. Determination of a suitable dose based on these factors is within the level of those skilled in the art, and the daily dose administered is, for example, 0.1 mg / kg / day to 100 mg / kg / day, more specifically 5 mg / kg / day to 50 mg / kg. / Can be, but is not limited to.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

[Comparative Example 1-1] Preparation of ginseng polysaccharide for chemical growth agent

The undried ginseng was put in a high-pressure steamer and steamed at a high temperature of 95 to 100 ° C for about 3 to 4 hours, and then the steamed ginseng having completed the above process step was dried at a temperature of 60 to 70 ° C in a hot air dryer. After crushing 1 kg of ginseng processed with a thickening agent with a blender, 10 l of purified water was added, stirred and extracted with hot water at 38-42 ° C. for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filtration and filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Here, ethanol of 4 times the concentrated amount was added dropwise and dried by hot air to obtain 70 g of polysaccharide processed ginseng polysaccharide using a foaming agent, a foaming technique.

[Comparative Example 1-2] Preparation of Green Tea Polysaccharides in Steam Enhancer

70 g of the green tea polysaccharide processed by the thickening method was obtained by using the same method as Comparative Example 1-1, except that the green tea leaves were subjected to primary processing (steaming process).

[Example 1-1] Preparation of ginseng and green tea polysaccharides in a thickening agent (mixed in a weight ratio of 1: 3)

25% by weight of undried ginseng and 75% by weight of primary processed (steaming process) green tea leaves are put in a high-pressure steamer and steamed at a high temperature of 95 to 100 ℃ for about 3 to 4 hours, followed by steaming that has completed the above process steps. The dried ginseng was dried in a hot air dryer at a temperature of 60 ~ 70 ℃. After crushing 1 kg of ginseng and green tea mixture processed with a thickening agent with a blender, 10 l of purified water was added, stirred and extracted with hot water at 38 to 42 ° C. for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filtration and filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Here, ethanol of 4 times the concentrated amount was added dropwise and dried by hot air to obtain 78 g of a mixture of ginseng polysaccharide and green tea polysaccharide processed by steaming using a foaming agent, a processing technique of a foaming agent.

[Example 1-2] Preparation of ginseng and green tea polysaccharides in a thickening agent (mixed in a weight ratio of 1: 1)

75 g of ginseng polysaccharide and green tea polysaccharide mixture processed by the same method as in Example 1-1, except that 50 wt% of undried ginseng and 50 wt% of primary processed (steaming process) green tea leaves were used. Got

[Example 1-3] Preparation of ginseng and green tea polysaccharides in a thickening agent (mixed in a weight ratio of 3: 1)

Excluding using 75% by weight of undried ginseng and 25% by weight of primary processed (steamed) green tea leaves, 70g of a mixture of ginseng polysaccharide and green tea polysaccharide processed by the same method as in Example 1-1 above Got

[Comparative Example 2-1] Preparation of ginseng salt polysaccharide

After mixing the dried ginseng well with brine (2 ~ 3%), seal it and let it absorb completely. Put the ginseng completely absorbed in the brine into the herb container at 100 ~ 180 ℃, and then fry it for 10 minutes ~ 1 hour. Dried. 10 g of purified water was added to the dried ginseng, refluxed 3 times, and then deposited at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated through filtration and filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. To this, 4 times of ethanol in a concentrated amount was added dropwise and dried by hot air to obtain 73 g of ginseng polysaccharide processed with salt.

[Comparative Example 2-2] Preparation of green tea salt polysaccharide

76 g of green tea polysaccharide was obtained by using the same method as Comparative Example 2-1, except that the green tea leaves were subjected to primary processing (steaming process).

[Example 2-1] Preparation of ginseng and green tea salt polysaccharide (mixed in a weight ratio of 1: 3)

72 g of dried ginseng polysaccharide and green tea polysaccharide mixture were obtained by using the same method as Comparative Example 2-1, except that 25 wt% of dried ginseng and 75 wt% of primary processed (dried process) green tea leaves were used. .

[Example 2-2] Preparation of ginseng and green tea salt polysaccharide (mixed in a weight ratio of 1: 1)

70 g of the ginseng polysaccharide and green tea polysaccharide mixture processed by the same method were obtained by using the same method as Comparative Example 2-1, except that 50% by weight of dry ginseng and 50% by weight of green tea leaves (primed process) were used. .

[Example 2-3] Preparation of ginseng and green tea salt polysaccharide (mixed in a weight ratio of 3: 1)

75 g of dried ginseng and 25 g of primary processed (steaming process) green tea leaves were used to obtain 75 g of a ginseng polysaccharide and green tea polysaccharide mixture processed in the same manner as in Comparative Example 2-1. .

[Comparative Example 3-1] Preparation of ginseng supertan polysaccharide

When the dried ginseng is carbonized outside at 230 ~ 300 ℃ and becomes black, the heating is stopped, cooled at room temperature, 10 liters of purified water is added to 1 kg of the obtained ginseng, extracted under reflux three times, and then deposited at 15 ℃ for 1 day. Thereafter, the residue and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration and centrifugation. Here, ethanol of 4 times the concentrated amount was added dropwise and dried by hot air to obtain 72 g of ginseng polysaccharide processed in super coal.

[Comparative Example 3-2] Preparation of green tea supertan polysaccharide

72 g of super-tanned green tea polysaccharide was obtained by using the same method as Comparative Example 3-1, except that the green tea leaves were subjected to primary processing (steaming process).

[Example 3-1] Preparation of ginseng and green tea supertan polysaccharide (mixed in a weight ratio of 1: 3)

Except for using 25% by weight of dried ginseng and 75% by weight of green tea leaves (primed process), 75 g of supergrated ginseng polysaccharide and green tea polysaccharide mixture was obtained using the same method as Comparative Example 3-1. .

[Example 3-2] Preparation of ginseng and green tea supertan polysaccharide (mixed in a weight ratio of 1: 1)

71 g of super grated ginseng polysaccharide and green tea polysaccharide mixture were obtained using the same method as Comparative Example 3-1, except that 50% by weight of dry ginseng and 50% by weight of green tea leaves (primed process) were used. .

[Example 3-3] Preparation of ginseng and green tea supertan polysaccharide (mixed in a weight ratio of 3: 1)

Except for using 75% by weight of dried ginseng and 25% by weight of primary processed (squeeze) green tea leaves, 70 g of supergrated ginseng polysaccharide and green tea polysaccharide mixture was obtained using the same method as Comparative Example 3-1. .

[Comparative Example 4-1] Preparation of ginseng herb polysaccharide

200 ~ 300g of vinegar was sufficiently absorbed in dried ginseng, and then roasted at 100 ~ 160 ℃ for 10 minutes ~ 1 hour, then dried in the shade. 10 g of purified water was added to 1 kg of ginseng thus obtained, extracted under reflux three times, and then deposited at 15 ° C for 1 day. Thereafter, the residue and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration and centrifugation. To this, 4 times of ethanol in a concentrated amount was added dropwise and dried by hot air to obtain 71 g of ginseng polysaccharide processed by virtue.

[Comparative Example 4-2] Preparation of green tea herb polysaccharide

73 g of super-processed green tea polysaccharide was obtained using the same method as Comparative Example 4-1, except that the green tea leaves were subjected to primary processing (steaming process).

[Example 4-1] Preparation of ginseng and green tea herbaceous polysaccharide (mixed in a weight ratio of 1: 3)

Except for using 25% by weight of dry ginseng and 75% by weight of green tea leaves (primed process), 76 g of a ginseng polysaccharide and green tea polysaccharide mixture prepared in the same manner as in Comparative Example 4-1 was obtained. .

[Example 4-2] Preparation of ginseng and green tea herbaceous polysaccharides (mixed in a weight ratio of 1: 1)

Except for using 50% by weight of dried ginseng and 50% by weight of primary processed (steaming process) green tea leaves, 74g of a mixture of ginseng polysaccharide and green tea polysaccharide processed by virtue was obtained in the same manner as in Comparative Example 4-1. .

[Example 4-3] Preparation of ginseng and green tea herb polysaccharide (mixed in a weight ratio of 3: 1)

Except for using 75% by weight of dry ginseng and 25% by weight of primary processed (steaming process) green tea leaves, 74g of a mixture of ginseng polysaccharide and green tea polysaccharide processed by virtue was obtained in the same manner as in Comparative Example 4-1. .

[Comparative Example 5-1] Preparation of ginseng main liquor polysaccharide

The dried ginseng was soaked in a certain amount of red wine to be moistened, put in a steamer, steamed for 30 minutes to 2 hours, and then dried in the shade. 10 g of purified water was added to 1 kg of ginseng thus obtained, extracted under reflux three times, and then deposited at 15 ° C for 1 day. Thereafter, the residue and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration and centrifugation. To this, 4 times of the concentrated amount of ethanol was added dropwise, and dried by hot air to obtain 77 g of ginseng polysaccharide processed as a main ingredient.

[Comparative Example 5-2] Preparation of Green Tea Primary Polysaccharide

75 g of green tea polysaccharides, whose main processing was performed, was obtained using the same method as Comparative Example 5-1, except that the green tea leaves were subjected to primary processing (steaming process).

[Example 5-1] Preparation of ginseng and green tea main polysaccharide (mixed in a weight ratio of 1: 3)

Except for using 25% by weight of dry ginseng and 75% by weight of green tea leaves (primed process), 74g of a mixture of ginseng polysaccharides and green tea polysaccharides, which were principally processed, was obtained using the same method as Comparative Example 5-1. .

[Example 5-2] Preparation of ginseng and green tea herbaceous polysaccharides (mixed in a weight ratio of 1: 1)

Except for using 50% by weight of dry ginseng and 50% by weight of primary processed (steaming process) green tea leaves, 77g of a mixture of ginseng polysaccharide and green tea polysaccharide processed in the main process was obtained using the same method as Comparative Example 5-1. .

[Example 5-3] Preparation of ginseng and green tea main polysaccharide (mixed in a weight ratio of 3: 1)

77 g of the ginseng polysaccharide and green tea polysaccharide mixture, which were principally processed, were obtained using the same method as in Comparative Example 5-1, except that 75 wt% of dried ginseng and 25 wt% of primary processed (steaming process) green tea leaves were used. .

[Comparative Example 6-1] Preparation of ginseng fermented polysaccharide

After crushing the fresh ginger, it was mixed with twice the water, squeezed and squeezed. This is repeated 2 or 3 times to prepare ginger juice, sprinkle 10 ~ 15% of ginger juice on dry ginseng weight, and evenly soak it, then put it in a container at 100 ~ 180 ℃ and soak it for 10 minutes ~ 1 hour. After dried in the shade. 10 g of purified water was added to 1 kg of ginseng thus obtained, extracted under reflux three times, and then deposited at 15 ° C for 1 day. Thereafter, the residue and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration and centrifugation. Here, ethanol of 4 times the concentrated amount was added dropwise and dried by hot air to obtain 69 g of fermented ginseng polysaccharide.

[Comparative Example 6-2] Preparation of green tea fermented polysaccharide

73 g of fermented green tea polysaccharide was obtained using the same method as in Comparative Example 6-1, except that the first processed green tea leaves were used.

[Example 6-1] Preparation of ginseng and green tea ferropolysaccharide (mixed in a weight ratio of 1: 3)

71 g of fermented ginseng polysaccharide and green tea polysaccharide mixture were obtained by using the same method as Comparative Example 6-1, except that 25 wt% of ginseng and 75 wt% of primary processed (steaming process) green tea leaves were used.

[Example 6-2] Preparation of ginseng and green tea ferropolysaccharide (mixed in a weight ratio of 1: 1)

70 g of a ginseng polysaccharide and a green tea polysaccharide mixture processed by fermentation were obtained using the same method as Comparative Example 6-1, except that 50 wt% of ginseng and 50 wt% of primary processed (dried process) green tea leaves were used.

[Example 6-3] Preparation of ginseng and green tea ferropolysaccharide (mixed in a weight ratio of 3: 1)

70 g of ginseng polysaccharide and green tea polysaccharide mixture obtained by fermentation were obtained using the same method as Comparative Example 6-1, except that 75 wt% of ginseng and 25 wt% of primary processed (steaming process) green tea leaves were used.

[Comparative Example 7-1] Preparation of ginseng wheat polysaccharide

The dried ginseng was sufficiently absorbed by 20-30% of honey, compared to the weight of ginseng, and then roasted at 100-160 ° C for 10 minutes to 1 hour, then dried in the shade. 10 g of purified water was added to 1 kg of ginseng thus obtained, extracted under reflux three times, and then deposited at 15 ° C for 1 day. Thereafter, the residue and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration and centrifugation. To this, 4 times of ethanol in a concentrated amount was added dropwise and dried by hot air to obtain 75 g of ginseng polysaccharide processed into wheat flour.

[Comparative Example 7-2] Preparation of green tea scoop polysaccharide

76 g of green tea polysaccharide was obtained by using the same method as in Comparative Example 7-1, except that the green tea leaves were subjected to primary processing (steaming process).

[Example 7-1] Preparation of ginseng and green tea wheat polysaccharide (mixed in a weight ratio of 1: 3)

Except for using 25% by weight of dry ginseng and 75% by weight of green tea leaves (primed process), 74g of a mixture of ginseng polysaccharide and green tea polysaccharide processed by milling was obtained using the same method as Comparative Example 7-1. .

[Example 7-2] Preparation of ginseng and green tea wheat polysaccharide (mixed in a weight ratio of 1: 1)

Except for using 50% by weight of dry ginseng and 50% by weight of primary processed (steaming process) green tea leaves, 75g of a mixture of ginseng polysaccharide and green tea polysaccharide processed into flour was obtained using the same method as Comparative Example 7-1. .

[Example 7-3] Preparation of ginseng and green tea wheat polysaccharide (mixed in a weight ratio of 3: 1)

Excluding using 75% by weight of dry ginseng and 25% by weight of primary processed (steaming process) green tea leaves, 74g of a mixture of ginseng polysaccharide and green tea polysaccharide processed by milling was obtained using the same method as Comparative Example 7-1. .

[Comparative Example 8-1] Preparation of polysaccharide extracted from ginseng products

After crushing 1 kg of dried ginseng with a blender, 10 liters of purified water was added thereto, and stirred and extracted with hot water at 38 to 42 ° C. for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filtration and filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Here, ethanol of 4 times the concentrated amount was added dropwise and dried by hot air to obtain 65 g of ginseng polysaccharide.

[Comparative Example 8-2] Preparation of polysaccharide extracted from green tea products

72 g of green tea polysaccharide was obtained using the same method as Comparative Example 8-1, except that 1 kg of green tea leaves, which were primarily processed (steaming process), was used.

[Comparative Example 8-3] Preparation of polysaccharide mixture extracted from ginseng and green tea products (mixed in a weight ratio of 1: 3)

A mixture of ginseng polysaccharide and green tea polysaccharide was prepared by simply mixing the ginseng polysaccharide and green tea polysaccharide prepared in Comparative Examples 8-1 and 8-2 in a ratio of 1: 3.

[Comparative Example 8-4] Preparation of polysaccharide mixture extracted from ginseng and green tea products (mixed in a weight ratio of 1: 1)

A mixture of ginseng polysaccharide and green tea polysaccharide was prepared by simply mixing the ginseng polysaccharide and green tea polysaccharide prepared in Comparative Examples 8-1 and 8-2 in a ratio of 1: 1.

[Comparative Example 8-5] Preparation of polysaccharide mixture extracted from ginseng and green tea products (mixed in a weight ratio of 3: 1)

A mixture of ginseng polysaccharide and green tea polysaccharide was prepared by simply mixing the ginseng polysaccharide and green tea polysaccharide prepared in Comparative Examples 8-1 and 8-2 in a ratio of 3: 1.

[Test Example 1] Tyrosinase inhibitory effect

The tyrosinase enzyme was extracted from mushrooms (SIGMA). First, the substrate tyrosine is dissolved in distilled water to make a solution of 0.3 mg / ml, and the solution is put into a test tube in 1.0 ml increments. Here, 1.0 ml of potassium-phosphate buffer solution (0.1 mol concentration, pH 6.8) and 0.7 ml of distilled water are added. Was added.

A mixture of the ginseng polysaccharide and green tea polysaccharide of Examples 1-1 to 7-3 or the ginseng polysaccharide and green tea polysaccharide of Comparative Examples 1-1 to 8-5 are mixed with ethanol solution at an appropriate concentration to react 0.2 ml of the sample solution. After putting it in the solution, it was reacted for 10 minutes in a constant temperature bath at 37 ° C. At this time, 0.2 ml of the solvent was prepared instead of each sample solution as a negative control (no addition), and ascorbic acid was used as a positive control. To the reaction solution, 0.1 ml of a 2500 unit / ml tyrosinase solution was added and reacted for 10 minutes in a 37 ° C. thermostat. The test tube containing the reaction solution was placed in ice water to quench the reaction to stop the reaction, and the absorbance at a wavelength of 475 nm was measured by a photospectroscopy, and the results are shown in Table 1 below. Each tyrosinase inhibitory effect was calculated by Equation 1 below.

Test substance Tyrosinase inhibition rate (%) Eumseong control (no additives) 0 Positive control (ascorbic acid) 52 Example 1-1 56 Example 1-2 58 Example 1-3 59 Example 2-1 54 Example 2-2 56 Example 2-3 59 Example 3-1 54 Example 3-2 56 Example 3-3 57 Example 4-1 52 Example 4-2 54 Example 4-3 56 Example 5-1 57 Example 5-2 59 Example 5-3 60 Example 6-1 56 Example 6-2 59 Example 6-3 61 Example 7-1 57 Example 7-2 60 Example 7-3 62 Comparative Example 1-1 50 Comparative Example 1-2 48 Comparative Example 2-1 50 Comparative Example 2-2 49 Comparative Example 3-1 48 Comparative Example 3-2 48 Comparative Example 4-1 47 Comparative Example 4-2 48 Comparative Example 5-1 50 Comparative Example 5-2 50 Comparative Example 6-1 51 Comparative Example 6-2 50 Comparative Example 7-1 51 Comparative Example 7-2 49 Comparative Example 8-1 48 Comparative Example 8-2 45 Comparative Example 8-3 52 Comparative Example 8-4 53 Comparative Example 8-5 57

In the results of Table 1, when the ginseng polysaccharide and the green tea polysaccharide are each contained, the tyrosinase inhibitory effect is insufficient, but when used together, it can be seen that the tyrosinase inhibitory effect is remarkably excellent. Therefore, it can be seen that the composition containing both the polysaccharide extracted from the ginseng and green tea subjected to foaming processing can provide a very good whitening effect.

[Test Example 2] Inhibition of melanin production using B16 / F10 melanoma cells

A mixture of the ginseng polysaccharide and green tea polysaccharide of Examples 1-1 to 7-3, or a sample containing 0.1% by weight of each of the ginseng polysaccharide and green tea polysaccharide of Comparative Examples 1-1 to 8-5 as a test substance, at a constant concentration B16 / F10 Melanoma cells (Korea Cell Line Bank) were added to the culture medium, cultured for 3 days, the culture medium was removed, washed with PBS, and the cells were dissolved with 1N NaOH to measure absorbance at 405 nm. Cells to which no test substance was added were used as a control to measure melanin production inhibition of each test substance compared to the melanin content in the control. Kojic acid, known as a melanin inhibitor, was used as a positive control. The inhibition rate of melanin production was calculated according to Equation 2 below, and the results are shown in Table 2 below.

Test substance Melanogenesis inhibition rate (%) Eumseong control (no additives) 0 Positive control group (Mt. Koji) 53 Example 1-1 55 Example 1-2 57 Example 1-3 61 Example 2-1 55 Example 2-2 56 Example 2-3 60 Example 3-1 54 Example 3-2 56 Example 3-3 58 Example 4-1 53 Example 4-2 55 Example 4-3 59 Example 5-1 57 Example 5-2 59 Example 5-3 62 Example 6-1 57 Example 6-2 60 Example 6-3 62 Example 7-1 58 Example 7-2 60 Example 7-3 64 Comparative Example 1-1 50 Comparative Example 1-2 48 Comparative Example 2-1 52 Comparative Example 2-2 49 Comparative Example 3-1 49 Comparative Example 3-2 48 Comparative Example 4-1 50 Comparative Example 4-2 48 Comparative Example 5-1 51 Comparative Example 5-2 50 Comparative Example 6-1 50 Comparative Example 6-2 50 Comparative Example 7-1 52 Comparative Example 7-2 50 Comparative Example 8-1 49 Comparative Example 8-2 48 Comparative Example 8-3 51 Comparative Example 8-4 53 Comparative Example 8-5 56

In the results of Table 2, when the ginseng polysaccharide and the green tea polysaccharide are each contained, the melanin production inhibitory effect is insufficient, but when used together, it can be seen that the melanin production inhibitory effect is remarkably excellent. Therefore, it can be seen that the composition containing both the polysaccharide extracted from the ginseng and green tea subjected to foaming processing can provide a very good whitening effect.

[Reference Example 1] Preparation of nutritional cream for formulation examples and comparative formulation examples

According to the composition of Table 3 to Table 7 to prepare a nutritional cream in a conventional manner (unit: weight%).

ingredient Formulation example 1-1 1-2 1-3 2-1 2-2 2-3 3-1 3-2 3-3 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Example 1-1 0.1 - - - - - - - - Example 1-2 - 0.1 - - - - - - - Example 1-3 - - 0.1 - - - - - - Example 2-1 - - - 0.1 - - - - - Example 2-2 - - - - 0.1 - - - - Example 2-3 - - - - - 0.1 - - - Example 3-1 - - - - - - 0.1 - - Example 3-2 - - - - - - - 0.1 - Example 3-3 - - - - - - - - 0.1 Hydrogenated vegetable oil 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10 pentastearate & behenyl alcohol & sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol & arachidyl glucoside 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetaryl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 stearate & glycerol oleate & propylene glycol 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50

ingredient Formulation example 4-1 4-2 4-3 5-1 5-2 5-3 6-1 6-2 6-3 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Example 4-1 0.1 - - - - - - - - Example 4-2 - 0.1 - - - - - - - Example 4-3 - - 0.1 - - - - - - Example 5-1 - - - 0.1 - - - - - Example 5-2 - - - - 0.1 - - - - Example 5-3 - - - - - 0.1 - - - Example 6-1 - - - - - - 0.1 - - Example 6-2 - - - - - - - 0.1 - Example 6-3 - - - - - - - - 0.1 Hydrogenated vegetable oil 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10 pentastearate & behenyl alcohol & sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol & arachidyl glucoside 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetaryl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 stearate & glycerol oleate & propylene glycol 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50

ingredient Formulation example Comparative Formulation Example 7-1 7-2 7-3 1-1 1-2 2-1 2-2 3-1 3-2 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Example 7-1 0.1 - - - - - - - - Example 7-2 - 0.1 - - - - - - - Example 7-3 - - 0.1 - - - - - - Comparative Example 1-1 - - - 0.1 - - - - - Comparative Example 1-2 - - - - 0.1 - - - - Comparative Example 2-1 - - - - - 0.1 - - - Comparative Example 2-2 - - - - - - 0.1 - - Comparative Example 3-1 - - - - - - - 0.1 - Comparative Example 3-2 - - - - - - - - 0.1 Hydrogenated vegetable oil 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10 pentastearate & behenyl alcohol & sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol & arachidyl glucoside 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetaryl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 stearate & glycerol oleate & propylene glycol 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50

ingredient Comparative Formulation Example 4-1 4-2 5-1 5-2 6-1 6-2 7-1 7-2 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Comparative Example 4-1 0.1 - - - - - - - Comparative Example 4-2 - 0.1 - - - - - - Comparative Example 5-1 - - 0.1 - - - - - Comparative Example 5-2 - - - 0.1 - - - - Comparative Example 6-1 - - - - 0.1 - - - Comparative Example 6-2 - - - - - 0.1 - - Comparative Example 7-1 - - - - - - 0.1 - Comparative Example 7-2 - - - - - - - 0.1 Hydrogenated vegetable oil 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10 pentastearate & behenyl alcohol & sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol & arachidyl glucoside 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetaryl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 stearate & glycerol oleate & propylene glycol 1.50 1.50 1.50 1.50 1.50 1.50 1.50 1.50

ingredient Comparative Formulation Example 8-1 8-2 8-3 8-4 8-5 9 Purified water to 100 to 100 to 100 to 100 to 100 to 100 Comparative Example 8-1 0.1 - - - - - Comparative Example 8-2 - 0.1 - - - - Comparative Example 8-3 - - 0.1 - - - Comparative Example 8-4 - - - 0.1 - - Comparative Example 8-5 - - - - 0.1 -   Hydrogenated vegetable oil 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10 pentastearate & behenyl alcohol & sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol & arachidyl glucoside 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetaryl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 stearate & glycerol oleate & propylene glycol 1.50 1.50 1.50 1.50 1.50 1.50

[Test Example 3] Skin tone improvement effect

In order to investigate the skin tone improvement effect of ginseng polysaccharide and green tea polysaccharide, sixty six subjects were divided into six groups of six subjects, and the nutrition of the formulation examples 1-1 to 7-3 and comparative formulation examples 1-1 to 9 was divided into the subjects of each group. After each cream was used (1 evening / day application, total 1 week), the degree of skin tone improvement was evaluated by using a Facial Stage DM-3 (Moritex, Japan) device. The skin tone improvement rate was determined by measuring the brightness and color of the skin as measured values of the brightness and color of the skin, and the results are shown in Table 8 below. The higher the brightness and color change value, the better the skin tone.

Skin tone improvement rate (%) Test substance Brightness (mean ± standard deviation) Color (average ± standard deviation) Formulation Example 1-1 13 ± 2.46 12 ± 2.37 Formulation Example 1-2 14 ± 2.24 13 ± 2.25 Formulation Example 1-3 15 ± 2.41 14 ± 2.75 Formulation Example 2-1 13 ± 2.05 12 ± 2.65 Formulation Example 2-2 14 ± 3.41 13 ± 2.05 Formulation Example 2-3 15 ± 2.83 14 ± 2.86 Formulation Example 3-1 13 ± 3.01 12 ± 1.98 Formulation Example 3-2 14 ± 2.68 13 ± 2.96 Formulation Example 3-3 15 ± 3.03 14 ± 2.82 Formulation Example 4-1 13 ± 2.57 12 ± 2.80 Formulation Example 4-2 14 ± 3.10 13 ± 2.31 Formulation Example 4-3 15 ± 2.76 14 ± 2.54 Formulation Example 5-1 13 ± 2.22 12 ± 2.40 Formulation Example 5-2 14 ± 2.42 13 ± 2.54 Formulation Example 5-3 15 ± 2.53 14 ± 2.49 Formulation Example 6-1 13 ± 2.82 12 ± 2.87 Formulation Example 6-2 14 ± 2.56 13 ± 2.71 Formulation Example 6-3 15 ± 2.61 14 ± 2.05 Formulation Example 7-1 13 ± 2.19 12 ± 2.72 Formulation Example 7-2 14 ± 2.95 13 ± 2.91 Formulation Example 7-3 15 ± 3.25 14 ± 2.53 Comparative Formulation Example 1-1 12 ± 2.26 11 ± 2.51 Comparative Formulation Example 1-2 11 ± 2.84 10 ± 2.29 Comparative Formulation Example 2-1 12 ± 2.60 11 ± 2.12 Comparative Formulation Example 2-2 11 ± 2.03 10 ± 2.95 Comparative Formulation Example 3-1 12 ± 3.20 11 ± 2.18 Comparative Formulation Example 3-2 11 ± 2.54 10 ± 2.32 Comparative Formulation Example 4-1 12 ± 2.64 11 ± 2.71 Comparative Formulation Example 4-2 11 ± 1.95 10 ± 2.02 Comparative Formulation Example 5-1 12 ± 2.39 11 ± 2.77 Comparative Formulation Example 5-2 11 ± 2.82 10 ± 2.56 Comparative Formulation Example 6-1 12 ± 2.69 11 ± 2.23 Comparative Formulation Example 6-2 11 ± 2.40 10 ± 2.35 Comparative Formulation Example 7-1 12 ± 2.27 11 ± 2.41 Comparative Formulation Example 7-2 11 ± 2.46 10 ± 2.39 Comparative Formulation Example 8-1 11 ± 2.02 10 ± 2.18 Comparative Formulation Example 8-2 10 ± 2.38 9 ± 2.16 Comparative Formulation Example 8-3 12 ± 3.04 11 ± 1.89 Comparative Formulation Example 8-4 13 ± 2.95 13 ± 2.12 Comparative Formulation Example 8-5 15 ± 3.25 13 ± 2.03 Comparative Formulation Example 9 5 ± 2.34 5 ± 2.05

In the results of Table 8, when the ginseng polysaccharide and the green tea polysaccharide are each contained, the skin tone improving effect is insignificant, but when these are used together, it can be seen that the skin tone improving effect is remarkably excellent. Therefore, it can be seen that a composition containing both polysaccharides extracted from ginseng and green tea subjected to foaming processing can provide a very good skin tone improving effect.

[Test Example 4] Measurement of UV protection index

In order to confirm the UVB blocking effect of the comparative examples and examples, the general method of reporting the sun protection factor (SPF) in the literature (J. Soc. Cosmet. Chem., 40, pp127-133, 1989) It was measured according to. For 30 women, the amount of ultraviolet light emitted from the solar simulator lamp to the test site was adjusted to 20-100 mj / cm 2 and irradiated to the back of the subject. To confirm the UV blocking effect of the sample, the sample was uniformly applied to the subject's back with a thickness of 2 μl / cm 2 and left to dry for 15 minutes. The UV irradiation window is fixed to the sample application sites of Examples 1-1 to 7-3 and Comparative Examples 1-1 to 8-5, and other areas except the irradiation window are blocked with UV rays using leather and towels. UV light was irradiated. After irradiating ultraviolet rays and determining the minimum erythema (MED) after 24 hours, the ultraviolet ray blocking effect was calculated according to Equation 3 below. The UVB blocking effect is represented by SPF, where SPF is defined as the amount of ultraviolet rays required to generate MED on the skin treated with the sunscreen for the amount of ultraviolet rays required to generate MED on the untreated skin, and the following equation Counted as 3. Therefore, it means that the higher the SPF, the better the UVB blocking effect. The results by the above method are shown in Table 9 below.

Test substance UV protection index (SPF) Control 8 Example 1-1 22 Example 1-2 25 Example 1-3 28 Example 2-1 24 Example 2-2 26 Example 2-3 29 Example 3-1 23 Example 3-2 25 Example 3-3 27 Example 4-1 24 Example 4-2 27 Example 4-3 29 Example 5-1 23 Example 5-2 26 Example 5-3 28 Example 6-1 24 Example 6-2 26 Example 6-3 28 Example 7-1 25 Example 7-2 27 Example 7-3 30 Comparative Example 1-1 16 Comparative Example 1-2 13 Comparative Example 2-1 17 Comparative Example 2-2 14 Comparative Example 3-1 16 Comparative Example 3-2 14 Comparative Example 4-1 18 Comparative Example 4-2 15 Comparative Example 5-1 16 Comparative Example 5-2 14 Comparative Example 6-1 16 Comparative Example 6-2 13 Comparative Example 7-1 18 Comparative Example 7-2 15 Comparative Example 8-1 15 Comparative Example 8-2 12 Comparative Example 8-3 24 Comparative Example 8-4 25 Comparative Example 8-5 28

As shown in Table 9 above, it can be seen that the ultraviolet ray blocking effect is remarkably superior when these are used together than when the ginseng polysaccharide and the green tea polysaccharide are each contained.

[Reference Example 2] Preparation of nutrient longevity of formulation examples and comparative formulation examples

According to the composition of Tables 10 to 14, nutrient makeup (mil lotion) was prepared by a conventional method (unit: wt%).

ingredient Formulation example 1-1 1-2 1-3 2-1 2-2 2-3 3-1 3-2 3-3 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Example 1-1 0.1 - - - - - - - - Example 1-2 - 0.1 - - - - - - - Example 1-3 - - 0.1 - - - - - - Example 2-1 - - - 0.1 - - - - - Example 2-2 - - - - 0.1 - - - - Example 2-3 - - - - - 0.1 - - - Example 3-1 - - - - - - 0.1 - - Example 3-2 - - - - - - - 0.1 - Example 3-3 - - - - - - - - 0.1 Citosterol 1.7 1.7 1.7 1.7 1.7 1.7 1.7 1.7 1.7 Polyglyceryl 2-oleate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Ceramide 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Seteares-4 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 cholesterol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Dicetyl phosphate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Concentrated glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Sunflower Oil 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Carboxyvinyl polymer 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Xanthan gum 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Preservatives, incense Proper Proper Proper Proper Proper Proper Proper Proper Proper

ingredient Formulation example 4-1 4-2 4-3 5-1 5-2 5-3 6-1 6-2 6-3 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Example 4-1 0.1 - - - - - - - - Example 4-2 - 0.1 - - - - - - - Example 4-3 - - 0.1 - - - - - - Example 5-1 - - - 0.1 - - - - - Example 5-2 - - - - 0.1 - - - - Example 5-3 - - - - - 0.1 - - - Example 6-1 - - - - - - 0.1 - - Example 6-2 - - - - - - - 0.1 - Example 6-3 - - - - - - - - 0.1 Citosterol 1.7 1.7 1.7 1.7 1.7 1.7 1.7 1.7 1.7 Polyglyceryl 2-oleate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Ceramide 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Seteares-4 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 cholesterol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Dicetyl phosphate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Concentrated glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Sunflower Oil 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Carboxyvinyl polymer 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Xanthan gum 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Preservatives, incense Proper Proper Proper Proper Proper Proper Proper Proper Proper

ingredient Formulation example Comparative Formulation Example 7-1 7-2 7-3 1-1 1-2 2-1 2-2 3-1 3-2 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Example 7-1 0.1 - - - - - - - - Example 7-2 - 0.1 - - - - - - - Example 7-3 - - 0.1 - - - - - - Comparative Example 1-1 - - - 0.1 - - - - - Comparative Example 1-2 - - - - 0.1 - - - - Comparative Example 2-1 - - - - - 0.1 - - - Comparative Example 2-2 - - - - - - 0.1 - - Comparative Example 3-1 - - - - - - - 0.1 - Comparative Example 3-2 - - - - - - - - 0.1 Citosterol 1.7 1.7 1.7 1.7 1.7 1.7 1.7 1.7 1.7 Polyglyceryl 2-oleate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Ceramide 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Seteares-4 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 cholesterol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Dicetyl phosphate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Concentrated glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Sunflower Oil 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Carboxyvinyl polymer 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Xanthan gum 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Preservatives, incense Proper Proper Proper Proper Proper Proper Proper Proper Proper

ingredient Comparative Formulation Example 4-1 4-2 5-1 5-2 6-1 6-2 7-1 7-2 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Comparative Example 4-1 0.1 - - - - - - - Comparative Example 4-2 - 0.1 - - - - - - Comparative Example 5-1 - - 0.1 - - - - - Comparative Example 5-2 - - - 0.1 - - - - Comparative Example 6-1 - - - - 0.1 - - - Comparative Example 6-2 - - - - - 0.1 - - Comparative Example 7-1 - - - - - - 0.1 - Comparative Example 7-2 - - - - - - - 0.1 Citosterol 1.7 1.7 1.7 1.7 1.7 1.7 1.7 1.7 Polyglyceryl 2-oleate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Ceramide 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Seteares-4 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 cholesterol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Dicetyl phosphate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Concentrated glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Sunflower Oil 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Carboxyvinyl polymer 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Xanthan gum 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Preservatives, incense Proper Proper Proper Proper Proper Proper Proper Proper

ingredient Comparative Formulation Example 8-1 8-2 8-3 8-4 8-5 9 Purified water to 100 to 100 to 100 to 100 to 100 to 100 Comparative Example 8-1 0.1 - - - - - Comparative Example 8-2 - 0.1 - - - - Comparative Example 8-3 - - 0.1 - - - Comparative Example 8-4 - - - 0.1 - - Comparative Example 8-5 - - - - 0.1 - Citosterol 1.7 1.7 1.7 1.7 1.7 1.7 Polyglyceryl 2-oleate 1.5 1.5 1.5 1.5 1.5 1.5 Ceramide 0.7 0.7 0.7 0.7 0.7 0.7 Seteares-4 1.2 1.2 1.2 1.2 1.2 1.2 cholesterol 1.5 1.5 1.5 1.5 1.5 1.5 Dicetyl phosphate 0.4 0.4 0.4 0.4 0.4 0.4 Concentrated glycerin 5.0 5.0 5.0 5.0 5.0 5.0 Sunflower Oil 10.0 10.0 10.0 10.0 10.0 10.0 Carboxyvinyl polymer 0.2 0.2 0.2 0.2 0.2 0.2 Xanthan gum 0.3 0.3 0.3 0.3 0.3 0.3 Preservatives, incense Proper Proper Proper Proper Proper Proper

[Test Example 5] Skin irritation evaluation

In order to determine whether skin irritation or allergic reaction occurred through observation of skin reactions of ginseng polysaccharide and green tea polysaccharide, a human body patch test was conducted according to the Korean Food and Drug Administration standard skin safety test method. Thirty women aged 20 to 35 years were selected as subjects to be tested and avoided the midline of the subject's back. After leaving for 1 hour, skin irritation was evaluated according to the following criteria. The results are shown in Tables 15 to 19 below.

Formulation example 1-1 1-2 1-3 2-1 2-2 2-3 3-1 3-2 3-3 +++ 0 0 0 0 0 0 0 0 0 ++ 0 0 0 0 0 0 0 0 0 + 0 0 0 0 One 0 0 0 One ± 10 9 9 9 9 11 9 10 9 - 10 11 11 11 10 9 11 10 10 (+++: strong trouble, ++: weak trouble, +: weak trouble, ±: normal,-: negative)

Formulation example 4-1 4-2 4-3 5-1 5-2 5-3 6-1 6-2 6-3 +++ 0 0 0 0 0 0 0 0 0 ++ 0 0 0 0 0 0 0 0 0 + One One 0 0 0 0 One One One ± 10 9 8 10 9 9 11 10 9 - 9 10 12 10 11 11 8 9 10 (+++: strong trouble, ++: weak trouble, +: weak trouble, ±: normal,-: negative)

Formulation example Comparative Formulation Example 7-1 7-2 7-3 1-1 1-2 2-1 2-2 3-1 3-2 +++ 0 0 0 0 0 0 0 0 0 ++ 0 0 0 0 0 0 0 0 0 + One 0 0 One 0 One One One One ± 11 11 10 10 10 9 10 8 8 - 8 9 10 9 10 10 9 11 11 (+++: strong trouble, ++: weak trouble, +: weak trouble, ±: normal,-: negative)

Comparative Formulation Example 4-1 4-2 5-1 5-2 6-1 6-2 7-1 7-2 +++ 0 0 0 0 0 0 0 0 ++ 0 0 0 0 0 0 0 0 + 0 One 0 0 One One One One ± 9 9 10 10 9 10 10 9 - 11 10 10 10 10 9 9 10 (+++: strong trouble, ++: weak trouble, +: weak trouble, ±: normal,-: negative)

Comparative Formulation Example 8-1 8-2 8-3 8-4 8-5 9 +++ 0 0 0 0 0 0 ++ 0 0 0 0 0 0 + 2 2 One One 0 2 ± 10 10 10 11 11 9 - 8 8 9 8 9 9 (+++: strong trouble, ++: weak trouble, +: weak trouble, ±: normal,-: negative)

As shown in Tables 15 to 19, when the ginseng polysaccharide and green tea polysaccharide of the present invention were used together, it was found that skin irritation was not caused. Therefore, it was confirmed that the composition of the present invention is safe without causing skin irritation, and thus can be used as a composition for external application for skin for blocking UV rays or a pharmaceutical composition.

From the above results, it is confirmed that the composition containing the polysaccharide extracted from the ginseng and green tea treated with the foaming agent according to the present invention improves skin tone, is effective in blocking UV rays, and is also excellent in stability, and is useful as a composition for whitening and UV blocking. Could.

Hereinafter, a formulation example of the composition according to the present invention will be described, but the pharmaceutical composition and the cosmetic composition may be applied in various dosage forms, which are not intended to limit the present invention, but only to be specifically described.

[Formulation Example 1] Soft capsule

150 mg of ginseng polysaccharide of Comparative Example 1-1, 150 mg of green tea polysaccharide of Comparative Example 1-2, 2 mg of palm oil, 8 mg of palm hardened oil, 4 mg of lead, and 6 mg of lecithin were mixed, and 400 capsules per capsule were filled according to a conventional method. Was prepared.

[Formulation Example 2] Tablet

After mixing 150 mg of ginseng polysaccharide of Comparative Example 1-1, 150 mg of green tea polysaccharide of Comparative Example 1-2, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate, and adding 40 mg of 30% ethanol to form granules, It was dried at 60 ° C and compressed into tablets using a tablet press.

[Formulation Example 3] Granules

150 g of ginseng polysaccharide of Comparative Example 1-1, 150 mg of green tea polysaccharide of Comparative Example 1-2, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch were mixed and 100 mg of 30% ethanol was added to form granules, and then dried at 60 ° C. The granules were formed and then filled into the fabric. The final weight of the contents was 1 g.

[Formulation Example 4] Flexible Cosmetics (skin lotion)

According to the composition shown in Table 20, a softening agent was prepared by a conventional method.

Ingredients Content (% by weight) Ginseng polysaccharide of Comparative Example 1-1 0.1 Green tea polysaccharide of Comparative Example 1-2 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 PG-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservatives, pigments, flavors Proper Purified water Balance

[Formulation Example 5] Nutritional Cosmetics (Milk Lotion)

According to the composition shown in Table 21, nutrient makeup was prepared by a conventional method.

Ingredients Content (% by weight) Ginseng polysaccharide of Comparative Example 1-1 0.5 Green tea polysaccharide of Comparative Example 1-2 0.5 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / Capric Triglyceride 5.0 Squalane 5.0 Sorbitasquioleate 1.5 Floating paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservatives, pigments, flavors Proper Purified water Balance

[Formulation Example 6] Nutrition Cream

Nutritional cream was prepared by a conventional method according to the composition shown in Table 22 below.

Ingredients Content (% by weight) Ginseng polysaccharide of Comparative Example 1-1 1.0 Green tea polysaccharide of Comparative Example 1-2 1.0 glycerin 3.0 Butylene glycol 3.0 Floating paraffin 7.0 Beta glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglyceride 3.0 Squalane 5.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservatives, pigments, flavors Proper Purified water Balance

[Formulation Example 7] Massage Cream

Massage creams were prepared in a conventional manner according to the composition shown in Table 23 below.

Ingredients Content (% by weight) Ginseng polysaccharide of Comparative Example 1-1 1.5 Green tea polysaccharide of Comparative Example 1-2 1.5 glycerin 8.0 Butylene glycol 4.0 Floating paraffin 45.0 Beta glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglyceride 3.0 Wax 4.0 Cetearyl Glucoside 1.5 Sesqui oleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservatives, pigments, flavors Proper Purified water Balance

[Formulation Example 8] Pack

Packs were prepared by conventional methods according to the compositions listed in Table 24 below.

Ingredients Content (% by weight) Ginseng polysaccharide of Comparative Example 1-1 0.1 Green tea polysaccharide of Comparative Example 1-2 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 suitable Preservatives, pigments, flavors Proper Purified water Balance

[Formulation Example 9] Ointment

Ointments were prepared in a conventional manner according to the composition shown in Table 25 below.

Ingredients Content (% by weight) Ginseng polysaccharide of Comparative Example 1-1 0.1 Green tea polysaccharide of Comparative Example 1-2 0.1 glycerin 8.0 Butylene glycol 4.0 Floating paraffin 15.0 Beta glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglyceride 3.0 Squalane 1.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservatives, pigments, flavors Proper Purified water Balance

Since the specific parts of the present invention have been described in detail above, it is obvious that for those skilled in the art, this specific technique is only a preferred embodiment, and the scope of the present invention is not limited thereby. something to do. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (10)

Ginseng root and ginseng root polysaccharide extracted from green tea leaves and ginseng root polysaccharide extracted from green tea leaves A composition for external application for skin for UV protection, comprising leaf polysaccharide as an active ingredient. According to claim 1, The content of each of the ginseng root polysaccharide and green tea leaf polysaccharide contained in the composition is characterized in that 0.001 to 10% by weight based on the total weight of the composition, the composition for external application for sunscreen for sun protection. According to claim 1, wherein the weight ratio of ginseng root polysaccharide and green tea leaf polysaccharide contained in the composition is characterized in that 0.1 to 10: 1, the composition for external application for skin protection against UV rays. According to claim 3, wherein the weight ratio of ginseng root polysaccharide and green tea leaf polysaccharide contained in the composition is characterized in that 1: 3, 1: 1, or 3: 1, the sunscreen composition for external sunscreen. According to claim 1,
The ginseng root and green tea leaves prepared by the salting method are absorbed by adding ginseng root and green tea leaves to the salt water, and then roasted for 10 minutes to 1 hour at a temperature of 100 to 180 ° C, and then dried in the shade. Will
The ginseng root and green tea leaves treated with the supertan foaming method are obtained by carbonizing ginseng root and green tea leaves at a temperature of 230 to 300 ° C.
The ginseng root and green tea leaves treated with the herbicide preparation method are obtained by putting ginseng root and green tea leaves in vinegar, absorbing the vinegar, and then frying them for 10 minutes to 1 hour at a temperature of 100 to 160 ° C., then drying them in the shade. Will
The ginseng roots and green tea leaves treated by the above-mentioned main-inhibition method are obtained by putting ginseng roots and green tea leaves into the red wine, absorbing the red wine, steaming in a steamer for 30 minutes to 2 hours, and drying in the shade.
The ginseng root and green tea leaves prepared by the fermentation method are absorbed in ginger juice by adding ginseng root and green tea leaves to ginger juice, and then roasted for 10 minutes to 1 hour at a temperature of 100 to 180 ° C, and then dried in the shade. Will
The ginseng root and green tea leaves prepared by the above-mentioned milling method were absorbed with honey by adding ginseng root and green tea leaves to honey, roasted at a temperature of 100-160 ° C for 10 minutes to 1 hour, and then dried and dried in the shade. The composition for external application for skin for UV protection, which is characterized in that it will. Ginseng root and ginseng root polysaccharide extracted from green tea leaves and ginseng root polysaccharide extracted from green tea leaves A pharmaceutical composition for blocking ultraviolet rays containing leaf polysaccharide as an active ingredient. The method of claim 6, wherein the content of each of the ginseng root polysaccharide and green tea leaf polysaccharide contained in the composition is characterized in that 0.001 to 10% by weight, based on the total weight of the composition, the pharmaceutical composition for UV protection. The method of claim 6, wherein the weight ratio of ginseng root polysaccharide and green tea leaf polysaccharide contained in the composition is characterized in that 0.1 to 10: 1, the pharmaceutical composition for sun protection. The method of claim 8, wherein the weight ratio of ginseng root polysaccharide and green tea leaf polysaccharide contained in the composition is 1: 3, 1: 1, or 3: 1, characterized in that the pharmaceutical composition for UV protection. The method of claim 6,
The ginseng root and green tea leaves prepared by the salting method are absorbed by adding ginseng root and green tea leaves to the salt water, and then roasted for 10 minutes to 1 hour at a temperature of 100 to 180 ° C, and then dried in the shade. Will
The ginseng root and green tea leaves treated with the supertan foaming method are obtained by carbonizing ginseng root and green tea leaves at a temperature of 230 to 300 ° C.
The ginseng root and green tea leaves treated with the herbicide preparation method are obtained by putting ginseng root and green tea leaves in vinegar, absorbing the vinegar, and then frying them for 10 minutes to 1 hour at a temperature of 100 to 160 ° C., then drying them in the shade. Will
The ginseng roots and green tea leaves treated by the above-mentioned main-inhibition method are obtained by putting ginseng roots and green tea leaves into the red wine, absorbing the red wine, steaming in a steamer for 30 minutes to 2 hours, and drying in the shade.
The ginseng root and green tea leaves prepared by the fermentation method are absorbed in ginger juice by adding ginseng root and green tea leaves to ginger juice, and then roasted for 10 minutes to 1 hour at a temperature of 100 to 180 ° C, and then dried in the shade. Will
The ginseng root and green tea leaves prepared by the above-mentioned milling method were absorbed with honey by adding ginseng root and green tea leaves to honey, roasted at a temperature of 100-160 ° C for 10 minutes to 1 hour, and then dried and dried in the shade. It characterized in that, the UV-resistant pharmaceutical composition.