KR102067698B1 - Composition for skin-whitening and UV protection containing polysaccharides extracted from processed Panax ginseng and Green tea - Google Patents

Abstract

The present invention relates to a whitening and sunscreen composition containing a ginseng polysaccharide and a green tea polysaccharide, and more particularly, a mixture of polysaccharides extracted from white ginseng or black ginseng prepared by processing ginseng and each polysaccharide extracted from green tea. The present invention relates to a composition having excellent whitening effect, ultraviolet ray blocking effect and skin damage prevention effect due to ultraviolet rays.

Description

Composition for skin-whitening and UV protection containing polysaccharides extracted from processed Panax ginseng and Green tea}

The present invention relates to a whitening and sunscreen composition containing a ginseng polysaccharide and a green tea polysaccharide, and more particularly, a mixture of polysaccharides extracted from white ginseng or black ginseng prepared by processing ginseng and each polysaccharide extracted from green tea. The present invention relates to a composition having excellent whitening effect, ultraviolet ray blocking effect and skin damage prevention effect due to ultraviolet rays.

Human skin color is determined by a number of factors, including the activity of melanocytes that make melanin, the distribution of blood vessels, the thickness of the skin, and the presence or absence of pigments in the body, such as carotenoids and bilirubin. The most important is a black pigment called melanin produced by the action of various enzymes such as cinnases. The formation of melanin pigment is influenced by genetic factors, physiological factors related to hormone secretion and stress, and environmental factors such as ultraviolet irradiation. Melanin is present in the skin and has an important function of protecting the body from ultraviolet rays, but it is known that melanin is excessively produced to form spots or freckles, promote skin aging, and also play an important role in inducing skin cancer.

Ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione or derivatives thereof in order to cure or alleviate skin pigmentation abnormalities such as blemishes, freckles or pigmentation, and excessive melanin deposition caused by UV exposure. In addition, substances having tyrosinase inhibitory activity have been used in cosmetics and pharmaceuticals, but their use is limited due to insufficient whitening effects, safety problems for skin, formulation and stability problems when formulated in cosmetics, and the like.

Ultraviolet rays contained in sunlight may cause erythema formation or melanin pigmentation in skin cells when excessively irradiated to the skin, which may cause blemishes and blemishes, and produce lipid peroxide by reacting with sebum secreted in the epidermis. Not only does it cause severe skin cancer, but it can also cause it. UV light is divided into UV-A (320-400nm), UV-B (280-320nm) and UV-C (200-280nm) depending on its wavelength. Ultraviolet rays are mostly absorbed in the atmosphere, so UV rays that directly affect the human body are known as UV-A and UV-B.

The sunscreen, which is used to prevent skin damage by UV rays, refers to a product that exhibits a sunscreen effect by applying a special substance to certain skin areas. The sunscreen effect is a unit called SPF (Sun Protection Factor). Use it to indicate the degree of blocking of the product. The UV Protection Index is calculated by the FDA (Food and Drug Administration) formula, which shows the minimum Erythema Dose (MED) of UV-B without sunscreen and UV-B after sunscreen. The ratio of minimum erythema amount is shown. That is, as a multiple of the extent to which the skin of the natural state blocks ultraviolet rays, the higher the value of the UV blocking index, the better the effect of blocking the sun, but the worse the fit to the skin and the higher the side effects.

Sunscreens are classified into chemical sunscreens and physical sunscreens. Chemical sunscreens that act as chemical absorption of ultraviolet rays are well known organic blockers such as cinnamic acid, salicylic acid, and benzophenone. Physical sunscreens, which act as a mechanism for physical scattering and shielding of ultraviolet rays, include titanium dioxide, zinc oxide, and the like. The same inorganic blockers are well known. In the case of chemical sunscreens, there is an advantage of excellent UV protection effect, but the wavelength range is narrow, sticky and bundled, the feeling of use is bad and can be absorbed into the skin in molecular form, causing skin irritation. In the case of physical sunscreens, the amount of transmitted ultraviolet light is reduced by reflecting and scattering ultraviolet light. Mainly, ultrafine inorganic compounds are used, and these materials are characterized by a very high refractive index and excellent heat resistance compared to organic material sunscreens. Skin irritation is relatively low compared to chemical sunscreens, but there is a disadvantage that causes a heavy feeling of use and whitening is not cosmetically appropriate (Patent No. 2003-0046103).

Various studies have been conducted on sunscreens to compensate for the disadvantages of chemical sunscreens and physical sunscreens. Specifically, a form in which chemical sunscreens and physical sunscreens are mixed in an appropriate ratio is a method currently applied to most sunscreen products, but there are fundamental disadvantages described above. In addition, the form of adsorbing titanium dioxide, which is an inorganic barrier agent on the surface of the inorganic material or the polymer particles, or containing an organic barrier agent inside the particle is supplemented in terms of skin safety, but has a disadvantage in that the manufacturing process is complicated and the UV blocking efficiency is not high. Finally, the inorganic particles such as titanium dioxide and zinc oxide are made of ultrafine particles to compensate for the disadvantages of existing inorganic blockers and have a high efficiency blocking effect, but this does not completely reduce the whitening phenomenon and the feeling of use as the particle size decreases This has the disadvantage of deteriorating. Organic and inorganic sunscreens made of such chemicals have become a problem because of their side effects, and therefore, they have become interested in natural sunscreens that are completely harmless to the human body.

As an example of the prior invention regarding natural sunscreens, natural hydroxyanthracene polyglycosides have been reported as sunscreens (Bader et al., Cosmetics & Toiletries, 96 (10), pp67, 1981), and the use of coffee oils as sunscreens Use has been reported in French Patent No. 2-479-688. Similarly, natural extracts of aloe, francula, senna and cascara containing anthraquinone derivatives have also been reported as useful sunscreens (Fox et al., Cosmetics & Toiletries, 102 (3), pp 41, 1987).

Accordingly, the present inventors processed ginseng to produce white ginseng or black ginseng and mixed with the polysaccharide extracted from green tea, and the skin whitening effect and the sun protection superior to the case of using these polysaccharides by synergistic effects, respectively. While providing an effect, the stimulation to the skin was found to be small and the present invention was completed.

Accordingly, an object of the present invention is to provide a composition exhibiting excellent skin whitening effect and sunscreen effect by simultaneously using polysaccharides extracted from white ginseng or black ginseng and green tea prepared by processing ginseng.

In order to achieve the above object, the present invention is made by processing ginseng white ginseng or black ginseng; And it provides a whitening and sunscreen composition containing a polysaccharide extracted from green tea as an active ingredient.

The composition of the present invention is capable of obtaining a polysaccharide in high yield by extracting a polysaccharide from white ginseng or black ginseng prepared by processing ginseng, and also provides an excellent skin whitening effect by simultaneously containing the ginseng polysaccharide and green tea polysaccharide, It is effective in preventing skin damage. Therefore, the composition containing the ginseng polysaccharide and the green tea polysaccharide reduces pigmentation symptoms such as blemishes and freckles, makes the skin color appear uniform and bright, has a very good sunscreen effect, and has no irritation to the skin. It may be usefully used as a composition or a pharmaceutical composition.

The composition of the present invention contains ginseng polysaccharide and green tea polysaccharide as an active ingredient, the ginseng polysaccharide is characterized in that extracted from ginseng or black ginseng prepared by processing ginseng.

Hereinafter, the present invention will be described in more detail.

The ginseng (Panax ginseng CA Meyer) polysaccharide used in the present invention is a pectin-like substance having a molecular weight of 34,600, and contains about 60% of galacturonic acid. Galactose and the like constitute a side chain. Ginseng used in the present invention is a plant belonging to the genus Ogapi and ginseng, ginseng in the present invention can be used without limitation to the type and lotus root (年根), can be used both dried and undried ginseng. In addition, ginseng in the present invention can be used without limitation in the area, specifically, the root of ginseng can be used.

In addition, the green tea polysaccharide used in the present invention is derived from green tea, and unlike the polysaccharides found in other plants, it is an acidic polysaccharide group, and is produced by combining sugar components and amino acids made through photosynthesis. Green tea in the present invention can be used without limitation in the type and cultivation days, it is possible to use both dried or not dried green tea. In addition, the green tea in the present invention can be used without limitation, specifically, the leaves of green tea can be used.

In the present invention, in order to increase the content in the ginseng extract of the polysaccharide used as an active ingredient in the present invention, the ginseng is processed into white ginseng or black ginseng, and the polysaccharide is extracted from it.

Hundred years old ginseng used in the present invention means dried ginseng after immersing ginseng at a high temperature, for example, ginseng is dried for 20 to 28 hours, more specifically 1 day in water 70 ~ 90 ℃, and thus, It is not limited.

As the black ginseng used in the present invention repeats the process of steaming fresh ginseng with steam three or more times, the color represents pale black brown or black dark brown, and preferably, by undergoing a process of augmentation vesicles that repeats nine times the steaming and drying process. It refers to black hemp, the manufacturing method may use a method known in the art, the method is not particularly limited.

Specifically, the green tea polysaccharide and ginseng polysaccharide of the present invention are prepared by preparing ginseng root or green tea leaf extract with water at 38-42 ° C., and then concentrating it, removing impurities with a filter, and then dropwise adding ethanol thereto and drying with hot air. can do.

The composition of the present invention may contain a ginseng polysaccharide and a green tea polysaccharide in an amount of 0.001 to 10% by weight based on the total weight of the composition, respectively, preferably a ginseng polysaccharide and green tea polysaccharide to 0.005 to 9.5% by weight relative to the total weight of the composition, respectively. , 0.01-9%, 0.03-8.5%, 0.05-8%, 0.07-7.5%, 0.09-7%, 0.1-6.5%, 0.3-6%, 0.5-5.5% or 0.7 It may be contained in an amount of ˜5% by weight.

In the present invention, ginseng polysaccharide and green tea polysaccharide are used by mixing in the range of 0.1 to 10: 1 by weight. In addition, in order to maximize the synergistic effect of the combination of the two components, preferably used to mix the ginseng polysaccharide and green tea polysaccharide in the range of 0.5 to 9.5: 1, 1 to 9: 1 or 1.5 to 8.5: 1 by weight ratio Can be.

The composition according to the present invention can be usefully used for whitening and preventing skin damage by ultraviolet rays.

The composition of the present invention may be formulated as an external composition for skin, in particular a cosmetic composition, and may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the compositions of the present invention may be provided in all formulations suitable for topical application, for example, emulsions obtained by dispersing an oil phase in a solution, an aqueous phase, emulsions obtained by dispersing an aqueous phase in an oil phase, suspensions, solids, gels, powders, It may be provided in the form of a paste, foam or aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may include other ingredients in addition to the above-mentioned materials within a range not impairing the main effect, preferably a synergistic effect on the main effect. In addition, the composition according to the present invention may further include a moisturizer, an emulsifier, a UV absorber, a preservative, a fungicide, an antioxidant, a pH adjuster, organic and inorganic pigments, flavoring, cooling agent or limiting agent. The blending amount of the above components can be easily selected by those skilled in the art within the range that does not impair the object and effect of the present invention, the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight relative to the total weight of the composition. .

In addition, the compositions of the present invention may be formulated as pharmaceutical compositions. When the composition according to the present invention is applied to a pharmaceutical product, it may be formulated as an oral or parenteral dosage form in the form of a solid, semi-solid or liquid by adding a commercially available inorganic or organic carrier to the active ingredient used in the present invention. , The pharmaceutical composition according to the invention can be administered orally, parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous.

Formulations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups, pellets and the like. In addition, the formulation for parenteral administration includes injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated by carrying out according to a conventional method, and at this time, surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffers, suspending agents, and other commercially available auxiliaries can be suitably used. have.

The dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight, pathology and severity of the subject to be administered, the route of administration or the judgment of the prescriber. Determination of a suitable dose based on these factors is within the level of one of skill in the art and its daily dosage may be, for example, 0.1 mg / kg / day to 100 mg / kg / day, more specifically 5 mg / kg / day to 50 mg / kg. May be, but is not limited to.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Comparative Example 1 Preparation of Hundred Years Ginseng Polysaccharide

Ginseng was soaked in water at 70-90 ° C. for 1 day, and dried to prepare 100 years ginseng. 1 kg of 100 g of prepared ginseng was pulverized with a blender, 10 liters of purified water was added thereto, stirred and extracted with 38-42 ° C. hot water for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were concentrated under reduced pressure to 1/10 of the purified water by filtration and centrifugation. Four times of concentrated ethanol was added dropwise thereto, followed by hot air drying, to obtain 70 g of 100 years ginseng polysaccharide.

Comparative Example 2 Preparation of Black Ginseng Polysaccharide

The ginseng was placed in a high pressure steamer at high temperature for 3 to 4 hours at 100 ° C., and then dried nine times in a hot air dryer at a temperature of 60 to 70 ° C. 1kg of processed ginseng was pulverized with a blender, 10 liters of purified water was added thereto, stirred and extracted with hot water at 38-42 ° C. for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation with a filter cloth, and the filtrate was concentrated under reduced pressure to 1/10 the amount of purified water. To this was added dropwise four times the concentrated ethanol and hot air dried to obtain 71 g of black ginseng polysaccharide.

Comparative Example 3 Preparation of Ginseng Polysaccharide

1 kg of dried white ginseng was pulverized with a blender, and 10 liters of purified water was added thereto, stirred and extracted with 38-42 ° C. hot water for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to 1/10 the amount of purified water. To this was added dropwise ethanol in a concentrated amount of 4 times and hot air dried to obtain 65 g of ginseng polysaccharide.

Comparative Example 4 Preparation of Green Tea Polysaccharide

1 kg of the first processed green tea leaves were pulverized with a blender, 10 liters of purified water was added thereto, stirred and extracted with hot water at 38-42 ° C. for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to 1/10 the amount of purified water. To this was added dropwise four times the concentrated ethanol and hot air dried to obtain 72g of green tea polysaccharide.

Comparative Example 5 Preparation of Mixture of Ginseng Polysaccharide and Green Tea Polysaccharide

The ginseng polysaccharide prepared in Comparative Example 3 and the green tea polysaccharide prepared in Comparative Example 4 were mixed at a ratio of 1: 1.

Example 1 Preparation of a Mixture of Perennial Ginseng Polysaccharide and Green Tea Polysaccharide

The perennial ginseng polysaccharide prepared in Comparative Example 1 and the green tea polysaccharide prepared in Comparative Example 4 were mixed at a ratio of 1: 3.

Example 2 Preparation of a Mixture of Perennial Ginseng Polysaccharide and Green Tea Polysaccharide

The hundred year ginseng polysaccharide prepared in Comparative Example 1 and the green tea polysaccharide prepared in Comparative Example 4 were mixed at a ratio of 1: 1.

Example 3 Preparation of Mixture of Perennial Ginseng Polysaccharide and Green Tea Polysaccharide

The 100-year-old ginseng polysaccharide prepared in Comparative Example 1 and the green tea polysaccharide prepared in Comparative Example 4 were mixed at a ratio of 3: 1.

Example 4 Preparation of a Mixture of Black Ginseng Polysaccharide and Green Tea Polysaccharide

The black ginseng polysaccharide prepared in Comparative Example 2 and the green tea polysaccharide prepared in Comparative Example 4 were mixed at a ratio of 1: 3.

Example 5 Preparation of Mixture of Black Ginseng Polysaccharide and Green Tea Polysaccharide

The black ginseng polysaccharide prepared in Comparative Example 2 and the green tea polysaccharide prepared in Comparative Example 4 were mixed at a ratio of 1: 1.

Example 6 Preparation of a Mixture of Black Ginseng Polysaccharide and Green Tea Polysaccharide

The black ginseng polysaccharide prepared in Comparative Example 2 and the green tea polysaccharide prepared in Comparative Example 4 were mixed in a ratio of 3: 1.

 Test Example 1 Tyrosinase Inhibitory Effect

Tyrosinase enzyme was extracted from the mushroom (Mushroom) was used as the Sigma (SIGMA). First, the substrate tyrosine was dissolved in distilled water to make a solution of 0.3 mg / ml, and the solution was added to the test tube by 1.0 ml. Then, 1.0 ml of potassium-phosphate buffer solution (0.1 mol concentration, pH 6.8) and 0.7 ml of distilled water were added thereto. Added.

0.2 ml of the sample solution prepared by mixing the polysaccharides of Examples 1 to 6 or Comparative Examples 1 to 5 at an appropriate concentration in an ethanol solution was added to the reaction solution and reacted for 10 minutes in a 37 ° C thermostat. In this case, 0.2 ml of solvent was added instead of each sample solution as a negative control group (no addition), and ascorbic acid was used as a positive control group. 0.1 ml of a tyrosinase solution of 2500 units / ml was added to the reaction solution and reacted for 10 minutes in a 37 ° C thermostat. The test tube containing the reaction solution was placed in ice water to quench the reaction to stop the reaction, and the absorbance at wavelength 475 nm was measured with a photospectrometer. The results are shown in Table 1 below. Each tyrosinase inhibitory effect was calculated by the following equation.

Test substance Tyrosinase inhibition rate (%) Control group (no addition) 0 Ascorbic acid 52 Example 1 54 Example 2 59 Example 3 61 Example 4 55 Example 5 57 Example 6 59 Comparative Example 1 49 Comparative Example 2 50 Comparative Example 3 48 Comparative Example 4 45 Comparative Example 5 53

In the results of Table 1, it can be seen that the use of 100 ginseng or black ginseng polysaccharides than the case of using ginseng polysaccharides, the tyrosinase inhibitory effect is more excellent due to the increase in the polysaccharide content. In addition, when the ginseng polysaccharide and the green tea polysaccharide are contained, the tyrosinase inhibitory effect is insignificant, but when used together, the tyrosinase inhibitory effect is remarkably excellent. Therefore, it can be seen that when mixed with polysaccharides of green tea ginseng or black ginseng can provide a very good whitening effect.

[Test Example 2] inhibitory effect of melanin production using B16 / F10 melanoma cells

Using the samples containing 0.1% by weight of the polysaccharides of Examples 1 to 6 or Comparative Examples 1 to 5, respectively, as a test substance, the mixture was added to the culture solution of B16 / F10 melanoma cells (Korea Cell Line Bank) at a constant concentration for 3 days. After removing the culture solution, washed with PBS and dissolved the cells with 1N NaOH absorbance at 405nm was measured. The cells without test substance were used as a control group, and the degree of inhibition of melanin production of each test substance was measured by comparing with the melanin content of the control group. To calculate the melanin production inhibition rate according to the following equation 2 and the results are shown in Table 2.

Test substance Melanogenesis inhibition rate (%) Control group (no addition) 0 Kojisan 53 Example 1 58 Example 2 60 Example 3 63 Example 4 55 Example 5 57 Example 6 60 Comparative Example 1 59 Comparative Example 2 55 Comparative Example 3 49 Comparative Example 4 48 Comparative Example 5 53

In the results of Table 2, when using the ginseng polysaccharides than the case of using ginseng polysaccharides it can be seen that the melanin production inhibitory effect due to the increase in the polysaccharide content. In addition, when the ginseng polysaccharide and the green tea polysaccharide each contain melanin production inhibitory effect is insufficient, when used together, it can be seen that the tyrosinase inhibitory effect is remarkably excellent. Therefore, it can be seen that when mixed with polysaccharides of green tea ginseng or black ginseng can provide a very good whitening effect.

Reference Example 1 Preparation of Nutritional Cream of Formulation Example and Comparative Formulation Example

Nutritional cream was prepared in a conventional manner according to the composition of Table 3 to Table 4 (unit: wt%).

ingredient Formulation example One 2 3 4 5 6 Purified water to 100 to 100 to 100 to 100 to 100 to 100 Example 1 0.1 - - - - - Example 2 - 0.1 - - - - Example 3 - - 0.1 - - - Example 4 - - - 0.1 - - Example 5 - - - - 0.1 - Example 6 - - - - - 0.1 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 Glycerol Stearate 1.00 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 1.00 1.00 1.00 1.00 Arachidil Behenyl Alcohol & Arachidil Glucoside 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerolate & Propylene Glycol 1.50 1.50 1.50 1.50 1.50 1.50

ingredient Comparative formulation example One 2 3 4 5 6 Purified water to 100 to 100 to 100 to 100 to 100 to 100 Comparative Example 1 0.1 - - - - - Comparative Example 2 - 0.1 - - - - Comparative Example 3 - - 0.1 - - - Comparative Example 4 - - - 0.1 - - Comparative Example 5 - - - - 0.1 - Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 Glycerol Stearate 1.00 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 1.00 1.00 1.00 1.00 Arachidil Behenyl Alcohol & Arachidil Glucoside 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerolate & Propylene Glycol 1.50 1.50 1.50 1.50 1.50 1.50

[Test Example 3] skin tone improvement effect

In order to examine the skin tone improvement effect of ginseng polysaccharide and green tea polysaccharide, 60 subjects were divided into 6 groups of 10 persons, and each of the subjects used the nutrition creams of Formulation Examples 1-6 and Comparative Formulation Examples 1-6 (evening). Once / day application, for a total of 1 week), facial stage DM-3 (Moritex, Japan) was used to evaluate the degree of skin tone improvement. Skin tone improvement rate was determined by the lightness and color measurement value of the skin and the change in the brightness and color of the skin, the results are shown in Table 5 below. The greater the brightness and color change, the better the skin tone.

Skin tone improvement rate (%) Test substance Brightness (mean ± standard deviation) Color (mean ± standard deviation) Formulation Example 1 13 ± 2.65 12 ± 2.18 Formulation Example 2 14 ± 2.21 13 ± 2.86 Formulation Example 3 15 ± 2.58 14 ± 2.35 Formulation Example 4 13 ± 2.04 12 ± 2.23 Formulation Example 5 14 ± 3.10 13 ± 3.21 Formulation Example 6 16 ± 2.85 14 ± 2.37 Comparative Formulation Example 1 12 ± 2.45 11 ± 3.36 Comparative Formulation Example 2 12 ± 2.94 11 ± 2.56 Comparative Formulation Example 3 11 ± 2.02 10 ± 2.18 Comparative Formulation Example 4 10 ± 2.38 9 ± 2.16 Comparative Formulation Example 5 13 ± 2.95 13 ± 2.12 Comparative Formulation Example 6 5 ± 2.34 5 ± 2.05

In the results of Table 5, when the ginseng polysaccharides and green tea polysaccharides each contain a skin tone improvement effect is insufficient, when using them together it can be seen that the skin tone improvement effect is remarkably excellent. Therefore, it can be seen that when mixed with polysaccharide and green tea ginseng or black ginseng can provide a very good skin tone improvement effect.

Test Example 4 UV Protection Index Measurement

In order to confirm the UVB blocking effect of the Comparative Examples and Examples, the UV protection index (SPF, Sun Protection Factor) is reported in the general method (J. Soc. Cosmet. Chem., 40, pp 127-133, 1989) Measured according to. Thirty female subjects were irradiated to the back of the test subject by adjusting the amount of ultraviolet light emitted from the solar simulator lamp to the test site to 20-100mj / cm 2. In order to confirm the UV blocking effect of the sample, the sample was uniformly coated with a thickness of 2 μl / cm 2 on the subject's back and left to dry for 15 minutes. The UV irradiation window was attached to the sample application sites of Examples 1 to 6 and Comparative Examples 1 to 5 to fix the UV irradiation window, and the other parts except the irradiation window were blocked by using a leather and a towel and irradiated with UV light. After 24 hours, the minimum erythema amount (MED) was determined, and the UV blocking effect was calculated according to Equation 3 below. The UVB blocking effect is expressed as SPF, where SPF is defined as the amount of UV light required to produce MED on the skin treated with the sunscreen, versus the amount of UV light required to produce MED on the untreated skin, Is calculated as 3. Therefore, higher SPF means better UVB blocking effect. The results by the method are shown in Table 6 below.

Test substance UV Protection Index (SPF) Control 8 Example 1 22 Example 2 24 Example 3 27 Example 4 23 Example 5 25 Example 6 28 Comparative Example 1 18 Comparative Example 2 17 Comparative Example 3 15 Comparative Example 4 12 Comparative Example 5 25

As shown in Table 6 above, it can be seen that the UV protection effect is remarkably excellent when using them together than when each containing the ginseng polysaccharide and the green tea polysaccharide.

REFERENCE EXAMPLE 2 Preparation of Nutrients for Formulations and Comparative Formulations

Nutritional longevity (wheat lotion) was prepared in a conventional manner according to the composition of Table 7 to Table 8 (unit: wt%).

ingredient Formulation example One 2 3 4 5 6 Purified water to 100 to 100 to 100 to 100 to 100 to 100 Example 1 0.1 - - - - - Example 2 - 0.1 - - - - Example 3 - - 0.1 - - - Example 4 - - - 0.1 - - Example 5 - - - - 0.1 - Example 6 - - - - - 0.1 Cytostolol 1.7 1.7 1.7 1.7 1.7 1.7 Polyglyceryl 2-oleate 1.5 1.5 1.5 1.5 1.5 1.5 Ceramide 0.7 0.7 0.7 0.7 0.7 0.7 Ceteares-4 1.2 1.2 1.2 1.2 1.2 1.2 cholesterol 1.5 1.5 1.5 1.5 1.5 1.5 Dicetylphosphate 0.4 0.4 0.4 0.4 0.4 0.4 Concentrated glycerin 5.0 5.0 5.0 5.0 5.0 5.0 Sunflower oil 10.0 10.0 10.0 10.0 10.0 10.0 Carboxy Vinyl Polymer 0.2 0.2 0.2 0.2 0.2 0.2 Xanthan Gum 0.3 0.3 0.3 0.3 0.3 0.3 Preservative, incense Quantity Quantity Quantity Quantity Quantity Quantity

ingredient Comparative formulation example One 2 3 4 5 6 Purified water to 100 to 100 to 100 to 100 to 100 to 100 Comparative Example 1 0.1 - - - - - Comparative Example 2 - 0.1 - - - - Comparative Example 3 - - 0.1 - - - Comparative Example 4 - - - 0.1 - - Comparative Example 5 - - - - 0.1 - Cytostolol 1.7 1.7 1.7 1.7 1.7 1.7 Polyglyceryl 2-oleate 1.5 1.5 1.5 1.5 1.5 1.5 Ceramide 0.7 0.7 0.7 0.7 0.7 0.7 Ceteares-4 1.2 1.2 1.2 1.2 1.2 1.2 cholesterol 1.5 1.5 1.5 1.5 1.5 1.5 Dicetylphosphate 0.4 0.4 0.4 0.4 0.4 0.4 Concentrated glycerin 5.0 5.0 5.0 5.0 5.0 5.0 Sunflower oil 10.0 10.0 10.0 10.0 10.0 10.0 Carboxy Vinyl Polymer 0.2 0.2 0.2 0.2 0.2 0.2 Xanthan Gum 0.3 0.3 0.3 0.3 0.3 0.3 Preservative, incense Quantity Quantity Quantity Quantity Quantity Quantity

[Test Example 5] Skin irritation evaluation

In order to investigate the skin safety of ginseng polysaccharide and green tea polysaccharide, that is, whether skin irritation or allergic reaction occurred, the human patch test was conducted according to the Korean Food and Drug Administration's standard skin safety test. Twenty women aged 20 to 35 years were selected as test subjects, and the nutrient lotion of Formulation Examples 1 to 6 and Comparative Formulation Examples 1 to 6 were applied to both sides, avoiding the midline of the subject, and for 1 hour. After it was left to stand, skin irritation was evaluated according to the following criteria. The results are shown in Tables 9 to 10 below.

Formulation example One 2 3 4 5 6 +++ 0 0 0 0 0 0 ++ 0 0 0 0 0 0 + One One 0 One One 0 ± 10 10 10 8 9 9 - 9 9 10 11 10 11 (+++: Strong Trouble, ++: Weak Trouble, +: Weak Trouble, ±: Normal,-: Negative)

Comparative formulation example One 2 3 4 5 6 +++ One 2 3 4 5 6 ++ 0 0 0 0 0 0 + 0 0 0 0 0 0 ± 2 2 2 2 One 2 - 8 8 10 10 11 9 (+++: Strong Trouble, ++: Weak Trouble, +: Weak Trouble, ±: Normal,-: Negative)

As shown in Table 9 to Table 10, when used together with the ginseng polysaccharide and green tea polysaccharide of the present invention did not cause skin irritation. Therefore, it was confirmed that the composition of the present invention can be used as an external preparation composition or composition for skin without causing skin irritation.

From the above results, it can be seen that when mixed with polysaccharides of green ginseng or black ginseng, it improves skin tone, is effective in blocking UV rays, and is excellent in terms of stability.

Hereinafter, the formulation example of the composition according to the present invention, but the pharmaceutical composition and cosmetic composition is applicable to various formulations, which is intended to explain in detail only, not intended to limit the present invention.

Preparation Example 1 Soft Capsule

150 mg of ginseng polysaccharide of Comparative Example 1, 150 mg of green tea polysaccharide of Comparative Example 2, 2 mg of palm oil, 8 mg of palm hardened oil, 4 mg of sulfur wax and 6 mg of lecithin were mixed and 400 mg per capsule was prepared according to a conventional method to prepare a soft capsule.

Preparation Example 2 Tablet

150 mg of ginseng polysaccharide of Comparative Example 1, 150 mg of green tea polysaccharide of Comparative Example 2, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate, 40 mg of 30% ethanol were added to form granules, and then dried at 60 ° C. It was compressed into tablets using a tableting machine.

Preparation Example 3 Granule

150 mg of ginseng polysaccharide of Comparative Example 1, 150 mg of green tea polysaccharide of Comparative Example 2, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch were mixed and 100 mg of 30% ethanol was added to form granules, followed by drying at 60 ° C to form granules. The next gun was filled. The final weight of the content was 1 g.

[Example 4] Softening Cosmetic (Skin Lotion)

According to the composition described in Table 11 below to prepare a flexible cosmetic water in a conventional manner.

Compounding ingredient Content (% by weight) Ginseng Polysaccharide of Comparative Example 1 0.1 Green Tea Polysaccharide of Comparative Example 2 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water Remaining amount

[Example 5] Nutritional Longevity (Milk Lotion)

Nutrients were prepared in a conventional manner according to the composition shown in Table 12.

Compounding ingredient Content (weight%) Ginseng Polysaccharide of Comparative Example 1 0.5 Green Tea Polysaccharide of Comparative Example 2 0.5 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Beeswax 4.0 Polysorbate 60 1.5 Caprylic / Capric Triglycerides 5.0 Squalane 5.0 Sorbitassquioleate 1.5 Liquid paraffin 0.5 Cetearyl Alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity Purified water Remaining amount

Preparation Example 6 Nutrition Cream

Nutritional cream was prepared in a conventional manner according to the composition shown in Table 13.

Compounding ingredient Content (% by weight) Ginseng Polysaccharide of Comparative Example 1 1.0 Green Tea Polysaccharide of Comparative Example 2 1.0 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Squalane 5.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water Remaining amount

[Example 7] Massage Cream

To prepare a massage cream in a conventional manner according to the composition described in Table 14.

Compounding ingredient Content (% by weight) Ginseng Polysaccharide of Comparative Example 1 1.5 Green Tea Polysaccharide of Comparative Example 2 1.5 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Beeswax 4.0 Cetearyl Glucoside 1.5 Sesqui oleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, flavoring Quantity Purified water Remaining amount

[Example 8] Pack

To prepare a pack in a conventional manner according to the composition described in Table 15.

Compounding ingredient Content (% by weight) Ginseng Polysaccharide of Comparative Example 1 0.1 Green Tea Polysaccharide of Comparative Example 2 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl Phenyl Ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 quantity Preservative, coloring, flavoring Quantity Purified water Remaining amount

[Example 9] Ointment

The ointment was prepared in a conventional manner according to the composition described in Table 16 below.

Compounding ingredient Content (% by weight) Ginseng Polysaccharide of Comparative Example 1 0.1 Green Tea Polysaccharide of Comparative Example 2 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Squalane 1.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Cetearyl Alcohol 1.0 Beeswax 4.0 Preservative, coloring, flavoring Quantity Purified water Remaining amount

As described above in detail specific parts of the present invention, it is apparent to those skilled in the art that these specific descriptions are merely preferred embodiments, and thus the scope of the present invention is not limited thereto. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (12)

Ginseng Root Polysaccharide Extracted from Hundred Years Ginseng; And whitening skin external composition comprising green tea leaf polysaccharide extracted from green tea leaf as an active ingredient. According to claim 1, wherein the content of each of the ginseng root polysaccharide and green tea leaf polysaccharide contained in the composition is characterized in that 0.001 to 10% by weight based on the total weight of the composition, the skin external preparation composition for whitening. According to claim 1, wherein the weight ratio of the ginseng root polysaccharide and green tea leaf polysaccharide contained in the composition, characterized in that 0.1 to 10: 1, the external composition for skin whitening. According to claim 3, The weight ratio of the ginseng root polysaccharide and green tea leaf polysaccharide contained in the composition is characterized in that 1: 3, 1: 1, or 3: 1, whitening skin external composition. The skin whitening composition for skin whitening according to claim 1, wherein the white ginseng is prepared by immersing ginseng root in water of 70 to 90 ° C. for 20 to 28 hours and then drying. Ginseng Root Polysaccharide Extracted from Hundred Years Ginseng; And a whitening pharmaceutical composition containing the green tea leaf polysaccharide extracted from the green tea leaf as an active ingredient. According to claim 6, The content of each of the ginseng root polysaccharide and green tea leaf polysaccharide contained in the composition, characterized in that 0.001 to 10% by weight based on the total weight of the composition, whitening pharmaceutical composition. The whitening pharmaceutical composition of claim 6, wherein the weight ratio of the ginseng root polysaccharide and the green tea leaf polysaccharide contained in the composition is 0.1 to 10: 1. The whitening pharmaceutical composition of claim 8, wherein the weight ratio of the ginseng root polysaccharide and the green tea leaf polysaccharide contained in the composition is 1: 3, 1: 1, or 3: 1. According to claim 6, The white ginseng is characterized in that the ginseng root was prepared by immersing in 70 ~ 90 ℃ water for 20 to 28 hours and then dried to dry, pharmaceutical composition for whitening. delete delete