KR20160084825A - composition for preventing and alleviating ultraviolet-induced skin damage and cosmetic comprising the same - Google Patents

Abstract

The present invention relates to a composition for preventing and ameliorating hypersensitive skin reaction due to exposure to ultraviolet light. More specifically, provided are a cosmetic composition for preventing and ameliorating hypersensitive skin reaction due to exposure to ultraviolet light and a cosmetic ingredient using the same. According to the present invention, the cosmetic composition prevents and ameliorates hypersensitive skin reaction and/or damage on skin caused by continuous exposure to ultraviolet light for a certain amount of time. To this end, the composition of the present invention includes a fermented extract of onion skin.

Description

[0001] The present invention relates to a composition for prevention and improvement of skin hypersensitivity reaction caused by exposure to ultraviolet rays, and a cosmetic composition using the composition for preventing and alleviating ultraviolet-

The present invention relates to an onion fermented extract having an effect of preventing and improving skin hypersensitivity due to exposure to ultraviolet rays. The present invention relates to an onion fermented extract having an effect of preventing skin irritation and / or skin damage such as redness, The present invention relates to a composition for preventing and improving skin irritation caused by exposure to ultraviolet rays which prevents and / or alleviates skin irritation and / or skin damage which may occur in skin by exposure to ultraviolet rays, a method for producing the same, and a cosmetic formulation using the same.

The rapid development of the IT industry and sports culture, the development of new forms of collective housing lifestyle and lifestyle tools, and the quality of life associated with health and beauty have been prioritizing the social environment, which has greatly increased skin damage in a variety of causes and forms. (UV-A) and ultraviolet B (UV-B) due to destruction of the ozone layer due to environmental pollution, as well as skin damage caused by burns such as disasters, And skin damage of the chondrocytes accompanied by aging of the skin caused by exposure.

As soon as the skin is exposed to heat, the protein is denatured, coagulated, damaged, and tissue necrosis is induced. Even at temperatures as low as 44 or lower, long-term contact destroys the tissue, and at 44 to 55, the destruction rate of the cell doubles with every 1 degree rise in temperature.

When skin burns, inflammation is naturally induced in the body. The clinical symptoms of inflammation itself disappear within 24 to 48 hours, but metabolism and immune response persist until skin regeneration, and various The continuous immune response by the mediator occurs, which causes various side effects for a long time. In other words, even if the site of burning is complete, the scars that remain after the treatment will have a prolonged inflammation, over proliferation of fibroblasts and over production of collagen fibers ). For example, hypertrophic scars (scars), skin deformations due to overgrowth of fibroblasts and collagen fibers, skin irritation, skin pruritus (pruritus), skin rash and pain resulting from neovascularization and overgrowth of inflammatory cells, And construction.

Korean Unexamined Patent Publication No. 10-2009-0030995 discloses a composition for the prevention or treatment of hyperhidrosis and keloid, which contains sialyl oligosaccharide as an active ingredient, as a therapeutic agent for burn treatment.

Korean Patent No. 10-1099550 of the prior art discloses a composition for burn treatment characterized by containing bee venom as an active ingredient.

Ultraviolet rays are divided into UV-A (320 ~ 400nm) and UV-B (290 ~ 320nm) according to the wavelength affecting the skin. UV-A permeates into the dermis of the skin to induce skin cancer or wrinkle, And skin irritation. UV-B penetrates to the epidermis of the skin and can cause erythema, freckles or edema. In addition, secondary side effects caused by ultraviolet rays cause skin irritation, skin pigmentation, skin soreness, skin pruritus (pruritus), and hyperpigmented scarring (scar).

To suppress such skin damage, there is a growing interest in ultraviolet screening, and a variety of ultraviolet screening products are on the market every year.

A sunscreen agent is a product that contains ingredients that scatter ultraviolet rays or absorb ultraviolet rays, and are largely divided into an inorganic sunscreen agent and an organic sunscreen agent.

Inorganic sunscreen agents physically scatter ultraviolet rays reaching the skin, blocking ultraviolet rays, and zinc oxide and titanium dioxide are representative.

The inorganic UV blocking agent is effective in blocking UVA (UVA), but it has a disadvantage that the skin is whitish due to opacity and it can block the pores and cause skin trouble.

Organic sunscreen agents absorb ultraviolet energy reaching the skin to block ultraviolet rays, and ethylhexylmethoxycinnamate and octocrylene are representative.

Organic UV blocking agents are effective for ultraviolet B (UVB) absorption, and unlike inorganic UV blocking agents, they have no whitening effect, but may cause irritation to sensitive skin.

Most of the sunscreen products sold on the market are mixed with inorganic and organic ingredients. Based on the results of official tests on the UV protection index, the Korean Food and Drug Administration After acquiring and selling.

These sunscreens should be applied to the skin 15 minutes before exposure to UV rays, and should be applied frequently at 2-hour intervals as they will be removed by sweat or clothing.

However, in reality it is not easy to add water every 2 hours, and because of the stickiness of most sunscreen products, repeated skin irritation can cause skin troubles due to discomfort and blockage with heat. Also, it is difficult to completely block the skin exposure of ultraviolet rays all day because it is easily erased by water in places such as beaches where the degree and time are different depending on a person's sweat.

On the other hand, ultraviolet rays have both sides of pros and cons because they have side effects such as skin irritation or skin aging mentioned above, and also have vitally important functions such as pigment formation for defending the living body, stimulation of vitamin D production which plays an important role in calcium metabolism have.

Therefore, it is necessary to develop a product that can be utilized for human body by minimizing the side effects of ultraviolet rays, while avoiding ultraviolet rays unconditionally, while maximizing its advantages.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a method and an apparatus for preventing skin irritation and / And a composition for preventing and improving skin hypersensitivity due to exposure.

The second problem to be solved by the present invention is to provide a cosmetic formulation for prevention and improvement of skin hypersensitivity due to exposure to ultraviolet ray which is superior in efficacy and safety to the conventional ultraviolet screening related products by including extracts extracted from natural products.

A third problem to be solved by the present invention is to provide a method for inhibiting HSP70 expression, inhibiting microvascular cell proliferation, inhibiting HSP70 expression in a skin microinvasive cell and / or the like in order to prevent and / or ameliorate skin hypersensitivity reaction and / Inhibition of migration, vasodilatation and / or angiogenesis.

The present invention for solving the above-mentioned first problem relates to a composition for preventing and improving skin hypersensitivity reaction by exposure to ultraviolet rays, which comprises an onion fermented extract.

According to a preferred embodiment of the present invention, a composition for preventing and improving skin irritation caused by exposure to ultraviolet rays is a composition selected from the group consisting of redness, skin pigmentation, skin pain, pruritus and hypertrophic scars And the like can be prevented and improved.

According to another preferred embodiment of the present invention, the composition for preventing or ameliorating the skin hypersensitivity reaction by exposure to ultraviolet rays may be any one of a cosmetic, a medicament and a quasi-drug.

According to another preferred embodiment of the present invention, the concentration of the onion fermentation extract of the composition for preventing and improving skin hypersensitivity reaction by exposure to ultraviolet rays may be 50 to 10,000 ppm.

According to another preferred embodiment of the present invention, the composition for preventing and improving skin hypersensitivity reaction by exposure to ultraviolet rays can inhibit the expression of Heat Shock Protein (HSP) 70 in skin cells.

According to another preferred embodiment of the present invention, a composition for preventing and improving skin hypersensitivity reaction by exposure to ultraviolet rays can inhibit the proliferation or migration of skin microvascular cells in the dermal layer.

According to another preferred embodiment of the present invention, a composition for preventing and improving skin hypersensitivity due to exposure to ultraviolet rays can inhibit vasodilation and angiogenesis of skin cells.

Next, the present invention for solving the above-mentioned second problem relates to a cosmetic formulation for preventing and improving skin hypersensitivity reaction by exposure to ultraviolet rays, and includes an onion fermented extract.

According to a preferred embodiment of the present invention, cosmetic formulations for preventing and improving skin irritation caused by exposure to ultraviolet rays may be emulsion, lotion, cream, lotion, essence, pack, gel or mist.

According to another preferred embodiment of the present invention, cosmetic formulations for preventing and improving skin irritation caused by exposure to ultraviolet rays include glycerin, titanium oxide, aluminum hydroxide, stearic acid Zinc oxide, dimethicone, ethylhexyl methoxycinnamate, 4-methylbenzylidene camphor, Quaternium-18 bentonite, and the like. Sodium chloride, phytosterol, octocrylene, and dimethicone / vinyl dimethicone crosspolymer. In addition, the present invention is not limited to these examples.

According to another preferred embodiment of the present invention, the cosmetic preparation for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet rays comprises 700 to 900 parts by weight of glycerin per 100 parts by weight of the onion fermented extract; 900 to 1100 parts by weight of titanium oxide, aluminum hydroxide or stearic acid; 300-500 parts by weight of zinc oxide or dimethicone; 900 to 1100 parts by weight of ethylhexyl methoxycinnamate; 500 to 700 parts by weight of 4-methylbenzylidene camphor; 100-260 parts by weight of Quaternium-18 Bentonite; 50 to 150 parts by weight of sodium chloride; 20 to 60 parts by weight of phytosterol; 500 to 700 parts by weight of octocrylene; Dimethicone or dimethicone / vinyl dimethicone crosspolymer in an amount of 500 to 700 parts by weight.

Next, the present invention for solving the above-mentioned third problem relates to a method for producing an onion fermented extract, comprising the steps of (1) preparing a medium containing dried pulverized onion broth, (2) (3) extracting the medium containing the fermented onion broth with a C ₁ -C ₄ alcohol solvent to obtain an alcohol solvent extract, and (4) extracting the obtained alcohol solvent extract with filtration And then drying it to obtain an onion fermented extract.

According to a preferred embodiment of the present invention, the medium of step (1) of the method of producing an onion fermented extract may contain 0.1-10% by weight of onion.

According to another preferred embodiment of the present invention, the medium of step (1) of the method of producing an onion fermentation extract comprises at least one selected from a carbon source, a nitrogen source and a trace element, and the carbon source is glucose glucose, at least one selected from the group consisting of sucrose, maltose, fructose, lactose, xylose, galactose and arabinose. And the nitrogen source may include at least one selected from yeast extract, peptone, beef extract, and soybean protein degradation product.

The trace element may be selected from the group consisting of sodium acetate, potassium phosphate, diammonium citrate, magnesium sulfate (MgSO ₄ ), manganese sulfate (MnSO ₄ ), zinc sulfate , ZnSO ₄ ) and copper sulfate (CuSO ₄ ).

According to another preferred embodiment of the present invention, the medium of step (1) of the method for producing fermented onion extract is 0.01 to 10% by weight of onion, 0.1 to 5% by weight of glucose, yeast extract 0.01 to 0.5% by weight of sodium acetate, 0.01 to 0.5% by weight of diammonium citrate, 0.1 to 2% by weight of magnesium sulfate, MgSO 4, ₄ ) 0.001 to 0.01 wt%, manganese sulfate (MnSO ₄ ) 0.001 to 0.05 wt%, zinc sulfate (ZnSO ₄ ) 0.0001 to 0.01 wt%, copper sulfate (CuSO ₄ ) % And balance of the purified water.

According to another preferred embodiment of the present invention, the step (1) of the method of producing an onion fermented extract may further include sterilizing the medium for 90 to 121 minutes for 15 to 30 minutes.

According to another preferred embodiment of the present invention, the lactic acid bacterium of the step (2) of the method of producing an onion fermented extract is selected from the group consisting of Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus plantarum at least one strain of lactobacillus plantarum, lactobacillus brevis, and lactobacillus acidophilus.

According to another preferred embodiment of the present invention, the inoculation amount of the lactic acid bacterium in the step (2) of the method of producing the onion fermented extract may be 10 ⁵ to 10 ⁶ cfu / ml at the initial stage of inoculation.

According to another preferred embodiment of the present invention, the alcohol solvent in step (3) of the method for producing fermented onion fermentation extract is an aqueous 20 to 90% by weight ethanol solution, and the extraction in step (3) And extracting for 12 to 72 hours.

According to another preferred embodiment of the present invention, the alcohol solvent extract filtered in the step (4) of the method for producing fermented onion fermented extract is vacuum-concentrated at a temperature of 40 to 80, and then subjected to vacuum drying, spray drying spray dry or freeze dry.

The composition for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet rays of the present invention can inhibit HSP70 expression, inhibit microvascular cell proliferation, inhibit microvascular cell migration, vasodilatation and / or angiogenesis in skin cells, When the formulation containing the composition of the present invention is applied to the skin, skin irritation and / or skin damage, which can be caused by continuous exposure to ultraviolet light for a certain period of time, can be prevented and improved.

Figure 1 is a graph showing the quercetin content of a typical onion.
FIG. 2 is a result of measuring cytotoxicity on a medium containing onion fermented extract according to a preferred embodiment of the present invention.
FIG. 3 shows the results of measuring the inhibitory activity of HSP70 on a medium containing onion fermented extract according to a preferred embodiment of the present invention.
FIG. 4 is a graph showing the activity of inhibiting microvascular cell proliferation in a medium containing onion fermented extract according to a preferred embodiment of the present invention.
FIG. 5 is a microscopic observation of microvascular cell migration inhibitory activity on a medium containing an onion fermented extract according to a preferred embodiment of the present invention.
FIG. 6 shows microscopic observation of vasodilation and angiogenesis inhibitory activity on a medium containing onion fermented extract according to a preferred embodiment of the present invention.
FIGS. 7 to 13 are graphs showing the degree of inhibition of skin pigmentation caused by exposure to ultraviolet rays after applying a cosmetic formulation for prevention and improvement of skin irritation caused by exposure to ultraviolet rays according to a preferred embodiment of the present invention, to be.

Hereinafter, the present invention will be described in more detail.

As mentioned above, when a side effect part of ultraviolet rays is mentioned at a large extent, ultraviolet rays-related products are needed which can be advantageously used in the human body by receiving the advantages of ultraviolet rays, without unconditionally avoiding ultraviolet rays.

Accordingly, the present invention sought to solve the above-mentioned problems through a composition for preventing and improving skin irritation caused by exposure to ultraviolet light. Accordingly, it is possible to provide a UV-blocking-related product containing the composition of the present invention, which is excellent in the suppression of HSP 70 expression, inhibition of microvascular cell proliferation, inhibition of microvascular cell migration, vasodilatation and / The present invention provides a composition for preventing or ameliorating a skin hypersensitivity reaction caused by exposure to ultraviolet rays, which can prevent and / or improve skin sensitization and / or skin damage which may be caused by exposure to ultraviolet rays for a predetermined period of time.

In addition, the composition for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet rays of the present invention includes an extract extracted from a natural product, that is, an extract obtained by fermenting onion, so that the cosmetic preparation containing the composition of the present invention can be used as a conventional ultraviolet- Excellent efficacy and safety.

The composition for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet rays of the present invention includes an onion fermented extract. Accordingly, the composition for preventing and improving skin irritation caused by exposure to ultraviolet rays of the present invention can be used for skin sensitization and / or skin damage, for example, redness, skin pigmentation, , Pruritus (itching), and hypertrophic scars (scarring) can be prevented and improved. Such skin hypersensitivity reaction and / or skin damage may be remnants or damage that may occur after treatment, even if the site of burning of the skin has been completed. For example, redness, skin pigmentation, pruritus, itching and pain can be caused by the formation of new blood vessels and overexpression of inflammatory cells, while hypertrophic scars (scarring) can result in prolonged inflammation, It can be caused by over proliferation of fibroblasts and over production of collagen fibers.

The composition for preventing or ameliorating the skin hypersensitivity reaction by exposure to ultraviolet rays of the present invention may be any one of a cosmetic, a medicine and a quasi-drug. In other words, the composition of the present invention can be used for cosmetic purposes, pharmaceutical use, or quasi-drug use.

As mentioned above, the composition for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet rays of the present invention can inhibit microvascular cell proliferation and / or microvascular cell migration in skin cells by including an onion fermented extract Inhibition of vasodilation and / or angiogenesis of skin microvascular cells, and suppression of expression of HSP (Heat Shock Protein) 70, which are as follows.

FIG. 3 is a graph showing the results of experiments in which the concentration of onion fermented extract was diluted 10 times as much as the concentration of onion fermented extract contained in cosmetics, medicines or quasi-drugs. The inhibitory activity of HSP 70 on the medium containing onion fermented extract was measured 3, it can be seen that the onion fermentation extract of the present invention has an effect of inhibiting HSP70 expression in skin cells.

HSP is a protein synthesized when cells, tissues or individuals are exposed to temperatures higher than the physiological temperature. TSP-b1 is activated by overexpression of HSP, inducing vasodilation and neovascularization, inducing inflammatory reaction, promoting fibroblast synthesis, Stimulation of collagen fiber synthesis and the like are continuously activated. In other words, the heat shock response increases the activity of TGF-b1 in hypertrophic scars (scars) accompanied by inflammatory reactions, resulting in a variety of side effects. 3, it can be seen that the onion fermentation extract of the present invention has an effect of inhibiting HSP70 expression in skin cells.

4 to 6 show results obtained by diluting the onion fermentation extract concentration 10 times or more with respect to concentration of onion fermentation extract which can be contained in cosmetics, medicines or quasi-drugs. In the medium containing onion fermentation extract, FIG. 4 shows that the onion fermentation extract of the present invention has an inhibitory effect on the microvascular cell proliferation, and the microvessel cells of the skin on the medium containing the onion fermentation extract of the present invention Referring to FIG. 5, in which the inhibitory activity on migration is observed through a microscope, it can be confirmed that the onion fermentation extract of the present invention has an effect of inhibiting microvascular cell migration. 6, which shows microscopic observation of vascular dilation and angiogenesis inhibitory activity of skin microvascular cells in the medium containing the onion fermentation extract of the present invention, Formation inhibitory effect can be confirmed.

In particular, when the concentration of onion fermentation extract of the present invention is 50 to 10,000 ppm, preferably 300 to 5,000 ppm, inhibition of HSP 70 expression, inhibition of microvascular cell proliferation, inhibition of microvascular cell migration, vasodilation and angiogenesis inhibition Effect.

Accordingly, when the onion fermented extract of the present invention is present at a concentration of 50 to 10,000 ppm, it may be very advantageous to maximize the inhibition of HSP70 expression, inhibition of microvascular cell proliferation, inhibition of microvascular cell migration, vasodilation and angiogenesis inhibition. If the concentration of onion fermentation extract of the present invention is less than 50 ppm, the effect of suppressing HSP 70 expression, inhibition of microvascular cell proliferation, inhibition of microvascular cell migration, vasodilation and angiogenesis inhibition are insufficient, There is a possibility of toxicity.

Furthermore, the present invention provides cosmetic formulation for preventing and improving skin hypersensitivity reaction by exposure to ultraviolet rays.

The cosmetic preparation for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet rays of the present invention may have a formulation of skin-adhesive type cosmetics such as lotion, lotion, cream, lotion, essence, pack, gel or mist.

In addition to the onion fermented extract, other ingredients may be selected and mixed according to the purpose of use. For example, it is possible to use as a blending component a preservative component, a moisturizer, an emollient agent, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorber, a sunscreen agent, a preservative, a bactericide, an antioxidant, a plant extract, Pigment, censer, purified water, and the like. However, the blending components are not limited thereto, and other blending components can be blended to the extent that the objects and effects of the present invention are not impaired.

The cosmetic composition for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet rays according to the present invention includes an onion fermented extract and contains glycerin, titanium oxide, aluminum hydroxide, stearic acid Zinc oxide, dimethicone, ethylhexyl methoxycinnamate, 4-methylbenzylidene camphor, Quaternium-18 bentonite, and the like. Sodium chloride, phytosterol, octocrylene, and dimethicone / vinyl dimethicone crosspolymer. In addition, the present invention is not limited to these examples. By including such components, the cosmetic formulation for preventing and improving skin hypersensitivity reaction by exposure to ultraviolet rays of the present invention can be used as an ultraviolet barrier-related product having the effect of the present invention.

In other words, cosmetic formulations for prevention and improvement of skin irritation caused by exposure to ultraviolet rays of the present invention, which may be used as products for ultraviolet shielding, may be used for the prevention and improvement of skin irritation, skin pigmentation, skin pain, pruritus and hypertrophic scars It is possible to prevent and / or ameliorate skin sensitization and / or skin damage, and also have an effect of blocking ultraviolet rays by the compounding ingredients.

The glycerin which moisturizes the skin may contain 700 to 900 parts by weight, preferably 750 to 850 parts by weight, based on 100 parts by weight of the fermented extract of onion fermentation. A thin film is formed on the skin surface as a physical ultraviolet ray blocking component, The titanium oxide, aluminum hydroxide or stearic acid which reflects may contain 900 to 1100 parts by weight, preferably 950 to 1050 parts by weight, based on 100 parts by weight of the onion fermented extract.

The zinc oxide and / or dimethicone, which is an ultraviolet light scattering agent, may contain 300 to 500 parts by weight, preferably 350 to 550 parts by weight, based on 100 parts by weight of the onion fermented extract, and the chemical ultraviolet absorbing component, ethylhexylmethoxy The cinnamate may contain 900 to 1100 parts by weight, preferably 950 to 1050 parts by weight, based on 100 parts by weight of the onion fermented extract.

The 4-methylbenzylidene camber, which is a chemical ultraviolet blocking ingredient, may contain 500 to 700 parts by weight, preferably 550 to 650 parts by weight, based on 100 parts by weight of the onion fermented extract, and may be a suspending agent (non-surfactant) The quaternium-18 bentonite used may comprise 100 to 260 parts by weight, preferably 150 to 210 parts by weight, based on 100 parts by weight of the onion fermented extract.

The sodium chloride used as a viscosity increasing agent may contain 50 to 150 parts by weight, preferably 75 to 125 parts by weight, based on 100 parts by weight of the onion fermented extract, and the plant sterol, which prevents skin water loss, 20 to 60 parts by weight, preferably 30 to 40 parts by weight, based on 100 parts by weight of the resin composition.

In addition, the chemical ultraviolet blocking ingredient, octoxylene, may contain 500 to 700 parts by weight, preferably 550 to 650 parts by weight, based on 100 parts by weight of the onion fermented extract, and used as a foam inhibitor, a skin protective agent, The dimethicone and / or dimethicone / vinyl dimethicone crosspolymer may contain 500 to 700 parts by weight, preferably 550 to 650 parts by weight, based on 100 parts by weight of the onion fermented extract.

In addition to the above materials, the cosmetic preparation for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet rays of the present invention may further include purified water, preservatives, and / or flavoring agents.

As described above, the cosmetic preparation for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet rays of the present invention is effective for inhibiting the expression of HSP (Heat Shock Protein) 70, inhibiting microvascular cell proliferation, inhibiting microvascular cell migration, And / or angiogenesis can be inhibited, and the effect of preventing and / or improving skin irritation and / or skin damage such as redness of skin, skin pigmentation, skin pain, pruritus (itching) and hypertrophic scars (scar) Which is as follows.

Referring to FIGS. 7 to 13, in which the cosmetic composition for prevention and improvement of skin hypersensitivity due to exposure to ultraviolet rays is applied to skin and then the degree of skin pigment deposition by ultraviolet exposure is measured, The skin pigmentation degree of the skin can be improved by applying the cosmetic formulation for prevention and improvement of the skin hypersensitivity reaction to the skin. That is, when the cosmetic preparation for prevention and improvement of the skin irritation caused by exposure to ultraviolet rays of the present invention is applied to the skin, it is possible to prevent and / or improve skin irritation, which is one of skin irritation and / or skin irritation. In other words, when the cosmetic preparation for prevention and improvement of the skin hypersensitivity reaction by exposure to ultraviolet rays of the present invention is applied to the skin, when the cosmetic formulation for prevention and improvement of the skin hypersensitivity reaction by exposure to ultraviolet rays of the present invention is not applied to the skin And / or redness of the skin can be prevented and / or ameliorated as compared with the case where a general ultraviolet screening-related product not containing the onion fermentation extract of the present invention is applied to the skin.

The composition for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet rays and the onion fermentation extract contained in the formulation are prepared by the following method.

The step of producing the onion fermented extract comprises the steps of (1) preparing a medium containing dried pulverized onion wax, (2) fermenting the onion wort by inoculating the medium with the lactic acid bacteria, (3) filtering the step of extracting the culture medium containing a C ₁ ~ C ₄ alcohol solvent, to give the alcohol solvent extract, and (4) the resulting alcohol solvent extracts were then dried and a step of obtaining a blood fermentation onion extract. Thus, the produced onion fermented extract has an excellent effect in inhibiting the expression of HSP (Heat Shock Protein) 70, inhibiting microvascular cell proliferation, inhibiting microvascular cell migration and / or angiogenesis in skin cells.

First, as step (1), a medium containing pulverized onion broth is prepared.

The onion contains quercetin 4-glucoside, quercetin 4,7-diglycoside, quercetin 3,7-diglycoside, quercetin agglomerate, Flavonoids such as quercetin aglycone, isorhamnetin monoglycoside and kaempferol monoglycoside are contained. Of these, 80% are quercetin aglycoside, quercetin aglycoside, Monoglycoside, and quercetin glycone have been reported.

Quercetin is a type of flavonoid, and it has been reported that these flavonoids inhibit neovascularization of skin as well as protect blood vessels and capillaries, inhibit lipid peroxidation, and inhibit allergies and skin diseases.

The onion used in step (1) of the present invention uses the onion blood among the onions. The term onion, which is the term used in the present invention, refers to the red crust of the onion. In other words, when manufacturing the medium containing the pulverized onion pod in step (1), only the outermost skin of the pulverized onion can be used for the onion pod contained in the medium.

As mentioned above, the onion contains a substance called quercetin, and the quercetin content of the onion is as shown in Fig. 1 is a graph showing the quercetin content of onion. Referring to FIG. 1, the outer skin of the onion, rather than the inner skin, contains an increasing amount of quercetin. Specifically, the quercetin content of the onion crust was 322.0 mg / 100 g, the inside of the crust was 27.2 mg / 100 g in the first ply, 14.0 mg / 100 g in the second ply, 11.5 mg / 100 g in the third ply, It contains 100g. As a result, the onion fermentation extract of the present invention using the onion blood, which is the outer shell of the onion without using the inner skin of the onion, contains a high concentration of quercetin. In other words, the use of onion blood in the present invention is effective in inhibiting the expression of HSP (Heat Shock Protein) 70, inhibiting microvascular cell proliferation, inhibiting microvascular cell migration and / or angiogenesis in skin cells, If the inner skin of the onion is used instead of the blood, it is possible to suppress the expression of HSP (Heat Shock Protein) 70, inhibit microvascular cell proliferation, inhibit microvascular cell migration and / or angiogenesis in the skin cells for the purpose of the present invention Can be difficult to achieve.

The onions used in the present invention may be used after being washed 8 to 12 times in a washing machine and then pulverized after drying and roasting for 60 to 100 hours for 8 to 12 hours.

The medium of step (1) may contain 0.1-10% by weight, preferably 2-8% by weight of onion broth.

The culture medium may include at least one selected from a carbon source, a nitrogen source, and trace elements.

The carbon source may be selected from the group consisting of glucose, sucrose, maltose, fructose, lactose, xylose, galactose and arabinose. Or more, and may preferably include glucose.

The nitrogen source may comprise at least one selected from yeast extract, peptone, beef extract, and soybean protein degradation product, and may preferably comprise a yeast extract.

Trace elements are sodium acetate, potassium phosphate, diammonium citrate, magnesium sulfate (MgSO ₄ ), manganese sulfate (MnSO ₄ ), zinc sulfate (zinc sulfate) (ZnSO ₄ ) and copper sulfate (CuSO ₄ ), and preferably at least one selected from the group consisting of sodium acetate, potassium phosphate, ammonium citrate, magnesium sulfate, manganese sulfate, zinc sulfate and copper sulfate . ≪ / RTI >

The medium of step (1) may be selected from the group consisting of onion pork, glucose, yeast extract, sodium acetate, potassium phosphate, diammonium citrate, magnesium sulfate, MgSO ₄ , manganese sulfate MnSO ₄ , zinc sulfate ZnSO ₄ , copper sulfate CuSO ₄ and the balance purified water.

As mentioned above, the medium of step (1) may contain 0.1-10% by weight, preferably 2-8% by weight of onion.

Next, the medium of step (1) may contain 0.1 to 5% by weight, preferably 0.5 to 2% by weight of glucose, and 0.1 to 2% by weight, preferably 0.3 to 2% by weight, % Of sodium acetate, 0.01 to 0.5% by weight, preferably 0.03 to 0.1% by weight of sodium acetate, and 0.01 to 0.2% by weight, preferably 0.05 to 0.2% by weight, of potassium phosphate And may contain 0.01 to 0.5% by weight, preferably 0.03 to 0.1% by weight, of ammonium citrate (diammonium citrate).

The medium of step (1) may contain 0.001 to 0.1% by weight, preferably 0.003 to 0.01% by weight of magnesium sulfate and 0.001 to 0.05% by weight, preferably 0.003 to 0.01% by weight, of manganese sulfate. And may contain 0.001 to 0.01% by weight, preferably 0.002 to 0.005% by weight of zinc sulfate, and 0.001 to 0.01% by weight, preferably 0.002 to 0.005% by weight, of copper sulfate.

Finally, the medium of step (1) may contain purified water. The purified water may contain 0.1 to 10% by weight of onion, 0.1 to 5% by weight of glucose, 0.1 to 2% by weight of yeast extract, 0.01 to 0.2 wt% of potassium phosphate, 0.01 to 0.5 wt% of sodium acetate, 0.01 to 0.5 wt% of ammonium diammonium citrate, 0.001 to 0.01 wt% of magnesium sulfate (MgSO ₄ ) 0.001 to 0.05% by weight of manganese sulfate (MnSO ₄ ), 0.0001 to 0.01% by weight of zinc sulfate (ZnSO ₄ ), and 0.0001 to 0.01% by weight of copper sulfate (CuSO ₄ ) . ≪ / RTI >

Preparing a medium containing the pulverized onion broth in the step (1), and sterilizing the medium. At this time, the sterilization treatment was carried out at a temperature of 90 to 121 For 15 to 30 minutes, preferably 100 to 110 for 20 to 25 minutes.

Next, in step (2), lactic acid bacteria are inoculated on the medium prepared in step (1) to ferment onion pods. The lactic acid bacteria inoculated in step (2) are cultured at a temperature of 25 to 45, for 12 to 72 hours, preferably 30 to 40, for 36 to 60 hours to ferment a medium containing onion blood, If it is less than 25, fermentation does not easily occur. If it exceeds 45, weak lactic acid bacteria may be killed.

The lactic acid bacteria may be selected from the group consisting of Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus brevis (Lactobacillus sp. lactobacillus brevis, and lactobacillus acidophilus.

In step (2), the inoculation amount of the lactic acid bacteria may be 10 ⁵ to 10 ⁶ cfu / ml at the initial stage of inoculation. If the initial number of lactic acid bacteria inoculated in step (2) is less than 10 ⁵ cfu / ml, the culture time of the lactic acid bacteria becomes longer, and 10 ⁶ cfu / ml If it is exceeded, it may be an economical burden to the production of seed bacterium.

Next, as the step (3), the medium containing the onion blood fermented in the step (2) is extracted with a C ₁ -C ₄ alcohol solvent to obtain an alcohol solvent extract. The alcohol solvent used as the extraction solvent of the medium containing onion blood may be any one alcohol selected from the group consisting of C ₁ -C ₄ lower alcohol, but ethanol may be preferably used. The alcohol solvent of the present invention means alcohol, and may be 100% alcohol or mixed with other solvent. In this case, water, methyl alcohol, ethyl acetate, chloroform, hexane and the like can be preferably used as other solvents. When water is used as a solvent for the alcohol solvent, 20 to 90% by weight, preferably 40 to 80% It is very advantageous to maximize the extraction efficiency. In other words, if less than 20% by weight of an aqueous solution of a C ₁ to C ₄ alcohol is used, the onion components contained in the alcohol can be reduced And if it exceeds 90% by weight, it may become an economical burden without increasing the amount of onion ingredients contained in the alcohol.

In extracting the culture medium containing the onion broth fermented in step (2) of the present invention with a C ₁ -C ₄ alcohol solvent, it is possible to extract the medium at room temperature, and those having ordinary skill in the art Various conventional extraction methods that are self-explanatory may be used. In addition, in the extraction of the culture medium containing the onion blood fermented in the step (2) with a C ₁ -C ₄ alcohol solvent, the extraction temperature is 10 to 40, preferably 20 to 30, and the extraction time is 12 To 72 hours, preferably 24 to 48 hours, and the number of times of extraction may be 1 to 5 times, preferably 2 to 3 times.

Next, as the step (4), the alcohol solvent extract obtained in the step (3) is filtered and dried to obtain an onion fermented extract.

The filtration in step (4) is performed to remove the cell component or the insoluble component contained in the alcohol solvent extract obtained in the step (3), and the filtration is carried out using a filter press or a membrane filter, the filtration method using a centrifuge can be carried out and various conventional filtration methods obvious to those skilled in the art can be carried out.

The alcohol solvent extract filtered in step (4) may be concentrated in vacuo at a temperature of 40-80, preferably 50-70.

The vacuum concentrated alcohol solvent extract is subjected to a drying step and may be dried by various conventional drying methods apparent to those skilled in the art and preferably by vacuum drying, They may be dried by spray drying or freeze drying.

The onion fermented extract according to the present invention produced by the above-described method can inhibit the expression of HSP (Heat Shock Protein) 70, inhibit microvascular cell proliferation, inhibit microvascular cell migration and / or angiogenesis in skin cells The effect is excellent, as described above.

Hereinafter, the present invention will be described in detail with reference to examples. However, these examples are intended to further illustrate the present invention, and the scope of the present invention is not limited to these examples.

[ Example ]

Example  One. Onion p  Fermented extract preparation

(One) Onion blood  Preparation of the containing medium

Remove the surface of the onion purchased from the corporation of Samnet Food and Agricultural Company, Inc. and obtain only the surface of onion (hereinafter referred to as onion pine) and wash it in the washing machine about 10 times. Thereafter, it is dried and roasted at 80 to 10 hours and then pulverized.

The medium containing the pulverized onion broth was prepared as shown in Table 1 below.

Ingredients Weight% (w / w) Ingredients Weight% (w / w) Crushed onion p 8 Magnesium sulfate 0.012 Glucose 0.5 Manganese sulfate 0.003 Yeast extract 0.5 Zinc sulfate 0.001 Potassium phosphate 0.25 Copper sulfate 0.001 Sodium acetate 0.12 Purified water 90.493 Ammonium citrate 0.12

The medium prepared as shown in Table 1 is sterilized at a temperature of 121 for 20 minutes.

(2) Onion pine  Fermentation

Lactobacillus rhamnosus, a lactic acid bacterium, was inoculated to the medium prepared in Example 1-1, and the lactic acid bacteria were cultured at a temperature of 38 for 48 hours to ferment onion pods. At this time, the inoculation amount of lactic acid bacteria is 10 ⁶ cfu / ml at the initial stage of inoculation.

(3) Extraction with an alcohol solvent to obtain an alcohol solvent extract

5.34 L of 95% by weight ethanol was added to 1 L of the medium containing the onion broth fermented in Example 1-2, and the final alcohol concentration was adjusted to 80% by weight. The resulting mixture was extracted at room temperature for 48 hours to obtain 6.34 L of an alcohol solvent extract ≪ / RTI >

(4) The alcohol solvent extract was filtered and dried to obtain Onion p  Obtain fermented extract

6.34 L of the alcohol solvent extract obtained in Example 1-3 was subjected to filter press to remove the cell component and the insoluble component. Thereafter, the mixture was concentrated in vacuo at a temperature of 60 ° C and then freeze-dried to obtain about 25g of onion fermentation extract.

Example  2. Onion p  Manufacture of UV protection products including fermented extracts

(1) 4 g of glycerin and 0.2 g of phytosterol are added to 71.9 g of purified water, and the mixture is heated to 80 to prepare an aqueous phase.

(2) 5 g of titanium oxide, 2 g of zinc oxide, 5 g of ethylhexyl methoxycinnamate, 3 g of 4-methylbenzylidene camphor, 3 g of quaternium-18 0.9 g of bentonite (Quaternium-18 Bentonite), 3 g of octocrylene and 3 g of dimethicone, and the mixture is heated to 80 to prepare an oil phase.

(3) To the oil phase prepared in (2), the water phase prepared in (1) is slowly added slowly to the vessel while stirring to disperse uniformly.

(4) After lowering the temperature to 45, 0.5 g of sodium chloride, 0.5 g of preservative, 0.5 g of fragrance, and 0.5 g of onion fermented extract are added to the container and stirred to prepare an ultraviolet screening-related product.

Comparative Example  One

Was prepared in the same manner as in Example 1 except for step (2) of Example 1 (fermentation of onion pod).

Comparative Example  2

In the same manner as in Example 1 except that the onion inner skin stripped of onion was used in step (1) of Example 1, and the step (2) of Example 1 (fermentation of onion sauce) was used.

Comparative Example  3

Was prepared in the same manner as in Example 1, except that 0% by weight ethanol, that is, 100% by weight of purified water was used as an extraction solvent instead of the 80% by weight ethanol aqueous solution.

Comparative Example  4

The same ingredients as in Example 2 were mixed except for the onion fermented extract included in Example 2 to prepare a product.

Preparation Example  1. Cell culture

For cell culture, human dermal microvascular endothelial cells (HDMECs) derived from human dermal tissue were purchased from Innoprot, Spain.

Microvessel cells were plated in 75T flasks and cultured under 37, 5 vol% CO ₂ . The culture medium used was 5% FBS (fetal bovine serum), 1 volume% antibiotic, 1% bFGF (basic fibroblast grouth factor) and ECM medium (endothelial cell medium-innoprot) containing EGF , And the culture medium was changed every 3 days. When 85% by volume of the cells were cultured in the flask, the cells were incubated. After one or more passages, microvessel cells in a stable state were used for the experiment.

Experimental Example  1: Cytotoxicity confirmation test

In a 96-well culture vessel, 200 쨉 l of microvascular cells cultured in Preparation Example 1 was added to each well at a concentration of 1 10 10 ⁴ cells / ml, and cultured for 24 hours under 37, 5 volume% CO ₂ for stabilization Respectively. For 24 hours under each well (well) onion blood by the fermentation extract is added by 50ppm, 100ppm, 200ppm, 300ppm, 500ppm and 800ppm concentration and then 37, 5 vol% CO ₂ conditions, produced in Example 1 in 24 hours after the culture vessel 20 μl of an MTS sample (CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay kit, Cat no. G5421, Promega) was added and reacted for 2 hours in a 37, 5 volume% CO ₂ incubator The absorbance was measured at 492 nm. The viability ratio of microvascular cells was calculated based on the control group to which the onion fermentation extract prepared in Example 1 was not added in the culture container, and the results are shown in FIG.

As can be seen in FIG. 2, the onion fermented extract added at a concentration of 800 ppm showed slight toxicity to microvascular cells, but it was not at a significant level. The onion paprika extract added at concentrations of 50 ppm, 100 ppm, 200 ppm, 300 ppm and 500 ppm It was confirmed that the fermented extract did not show toxicity to microvascular cells.

FIG. 2 is a cytotoxicity experiment carried out at a concentration 10 times thinner than the concentration of the onion fermentation extract which can be contained in cosmetics, medicines or quasi-drugs. In the production of cosmetics, medicines or quasi-drugs including onion fermented extract Considering the cytotoxicity, it can be deduced that it is advantageous to use it at 5000 ppm or less.

Experimental Example  2 : HSP ( Heat Shock Protein ) Expression Inhibitory Effect Identification Test

Fibroblast and HaCat cells were cultured in the same manner as Preparation Example 1, respectively. Thereafter, the cultured Fibroblast and HaCat cells were seeded in each well of a 96-well culture container at 5 to 10 ⁴ cells / well, and each sample was added to each well of the culture container. . After incubation for 24 hours under 37, 5% by volume CO ₂ , RNA extraction was performed to confirm the inhibition of HSP 70 expression.

Each well was incubated with a well in which the fermented extract of Onion fermentation prepared in Example 1 of the present invention was added as a sample at concentrations of 100 ppm and 500 ppm, respectively, and the onion prepared in Comparative Example 1 The extracts were wells added at concentrations of 100 ppm and 500 ppm, respectively.

The results of inhibition of HSP70 expression in Fibroblast and HaCat cells in each well are shown in FIG.

As shown in FIG. 3, in the well-treated Fibroblast and HaCat cells treated with the concentrations of 100 ppm and 500 ppm, respectively, of the fermented onion extract prepared in Comparative Example 1, HSP 70 was expressed in almost the same manner as the control group, It was confirmed that the expression of HSP 70 was significantly inhibited in the wells of Fibroblast and HaCat cells treated with the fermented extract of onion of Example 1 at concentrations of 100 ppm and 500 ppm.

Based on this, it can be seen that the onion fermented extract of the present invention can remarkably suppress the expression of HSP70 in skin cells.

FIG. 3 is a graph showing the HSP expression inhibition experiment conducted at a concentration 10 times thinner than the concentration of onion fermented extract that can be contained in cosmetics, medicines or quasi-drugs. In the case of producing cosmetic products, medicines or quasi-drugs including onion fermented extract , It can be deduced that it is advantageous to use it at 5000 ppm or less in consideration of the suppression surface of HSP expression of skin cells.

Experimental Example  3: Microvascular cell  Proliferation inhibition confirmation test

96-well cultured for 24 hours under each well (well) 37, 5 vol% CO ₂ condition in order to stabilize the frequency division by 200㎕ in the microvascular cell culture prepared in Example 1 in the culture vessel so that the 1? 10 ⁴ cell / Respectively. After culturing for 24 hours, discard the medium of the upper layer and wash the lower layer medium containing microvascular cells with PBS (Phosphate buffer saline). After washing, 180 .mu.l of a medium containing 5% FBS (fetal bovine serum) was added to the well of the negative control, and 5% FBS and 2 ng / ml bFGF (basic fibroblast grouth factor) , And 2 ng / ml of EGF (epidermal growth factor).

Add 20 μl of sample to each well except the negative control wells of the 96-well culture vessel. Thereafter, a 96-well culture plate 37, and cultured for 48 hours in a 5% CO ₂ condition. After 48 hours, the upper layer of the medium was discarded, the lower layer containing microvessel cells was washed with 5% by volume of PBS, and the medium was replaced with DMEM (Dulbecco's modified eagle's medium) containing 5% FBS. 20 μl of a sample (CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay kit, Cat no. G5421, Promega) was added thereto, and reacted for 2 hours in a 37, 5% by volume CO ₂ incubator. Absorbance was measured at 492 nm Respectively. The survival ratio of cells in each well was then calculated based on the negative control wells.

Each well was a well in which the fermented extract of onion fermentation prepared in Example 1 of the present invention was added as a sample at concentrations of 50 ppm, 100 ppm, 200 ppm, 300 ppm, 400 ppm, and 500 ppm, respectively, as a positive control well A well in which the fermented onion fermentation extract prepared in Example 1 was not added as a sample, a well in which the extract of the inner shell of the onion prepared in Comparative Example 2 was added as a sample at concentrations of 100 ppm and 200 ppm, 3 was added to the wells at concentrations of 100 ppm and 200 ppm, respectively.

The results of measuring cell proliferation inhibitory activity of microvascular cells in each well are shown in FIG.

As can be seen from FIG. 4, when the fermented extract of fermented onion of Example 1 was added at a concentration of 50 to 500 ppm, the wells significantly inhibited the excessive proliferation of microvascular cells when compared with the positive control group And showed a cell proliferation inhibitory effect higher than that of the well to which Comparative Example 2 or Comparative Example 3 was added.

On the basis of this, it can be seen that the onion fermentation extract of the present invention can inhibit cell proliferation of skin microvascular cells. In particular, when the onion fermentation extract of the present invention is added at a concentration of 100 ppm to 500 ppm, Can be greatly suppressed.

By inhibiting the proliferation of microvascular cells overexpressed by stimulation, it can reduce the redness of skin caused by this.

FIG. 4 is a graph showing cell proliferation inhibition experiments at a concentration 10 times more diluted than the concentration of the onion fermented extract that can be contained in cosmetics, medicines or quasi-drugs. In the production of cosmetics, medicines or quasi-drugs including onion fermented extract Considering the cell activation surface and the like, it can be deduced that it is advantageous to use it at 5000 ppm or less.

Experimental Example  4 : Microvascular cell  Cell migration inhibition confirmation test

In a 96-well culture vessel, 200 쨉 l of microvessel cells cultured in Preparation Example 1 was added to each well at a concentration of 1 10 10 ⁵ cells / well and cultured for 15 hours under 37, 5% by volume CO ₂ for stabilization Respectively. After incubation for 15 hours, the cells were scratched with 200 μl tip and washed with PBS. Negative control 180 μl of a medium containing 5% FBS (fetal bovine serum) was added to the wells, and 5% FBS, bFGF (basic fibroblast grouth factor) 2 ng / ml and EGF epidermal growth factor) 2 ng / ml.

Add 20 μl of sample to each well except the negative control wells of the 96-well culture vessel. The 96-well culture vessel was then incubated for 9 hours under 37, 5% by volume CO ₂ conditions. Then, the degree of cell migration was observed through a microscope (Olympus IX53-100 magnification).

 In each well, the onion fermentation extract prepared in Example 1 of the present invention was added as a sample at concentrations of 0, 100 ppm, 200 ppm, 300 ppm, and 500 ppm.

The results of measuring the cell migration inhibitory activity of microvascular cells in each well are shown in FIG.

As can be seen from FIG. 5, when the fermented extract of Onion Fermentation Extract of Example 1 was added at a concentration of 100 to 500 ppm, the movement of microvascular cells was significantly inhibited when compared with the positive control, there was.

Based on this, it can be seen that the onion fermentation extract of the present invention can inhibit the cell migration of the microvascular cells of the skin, and if the movement of the blood vessel cells which are pushed around the damaged skin is suppressed, the skin redness around the damaged skin can be reduced have.

FIG. 5 shows a cell migration inhibition experiment conducted at a concentration 10 times more diluted than the concentration of onion fermented extract that can be contained in cosmetics, medicines or quasi-drugs. In the case of manufacturing cosmetic preparations, medicines or quasi-drugs including onion fermented extract Considering the cell activation surface and the like, it can be deduced that it is advantageous to use it at 5000 ppm or less.

Experimental Example  5: Verification test for vasodilation and angiogenesis inhibition

100 쨉 l of a cell adhesion protein (Growth factor reduced matrigel) was dispensed into a 96-well culture container and coated at 37 for 30 minutes.

Negative control wells of the 96-well culture vessels were diluted with 5% FBS (fetal bovine serum) -substituted microvessel cells and added to each well at a concentration of 3.6 - 10 ⁴ cells / frequency divider and a positive control with 5 vol% FBS with (basic fibroblast grouth factor) bFGF 2 ng / ㎖, EGF (epidermal growth factor) 2 ng / ㎖ microvascular cells into the medium containing the diluted to 3.6? 10 ⁴ cell / And 450 [mu] l of each solution is dispensed into each well. Then add 50 μl of each sample. Then, the 96-well culture vessel was incubated for 24 hours under the condition of 37, 5% by volume CO ₂ , and the degree of vasodilation and angiogenesis was observed through a microscope (Olympus IX53-100 magnification).

The wells are the wells in which the fermented extract onion fermentation prepared in Example 1 of the present invention was added as a sample at concentrations of 0, 100 ppm, 200 ppm, 300 ppm, and 500 ppm.

The results of measuring vascular dilation and angiogenesis inhibitory activity of microvascular cells in each well are shown in FIG.

As shown in FIG. 6, microscopic observation was made on inhibition of blood vessel dilation and angiogenesis of microvascular cells in wells added with concentration of 100 ppm to 500 ppm of the fermented extract of onion fermentation of Example 1.

Based on this, it can be seen that the onion fermented extract of the present invention can inhibit vascular expansion and angiogenesis of skin cells.

FIG. 6 is a graph showing blood vessel dilation and blood vessel formation inhibition at a concentration 10 times more diluted than the concentration of onion fermented extract, which can be contained in cosmetics, medicines or quasi-drugs, and is a cosmetic composition containing a fermented onion extract, It can be deduced that it is advantageous to use it at 5000 ppm or less in consideration of the cell activity and the like.

As described above, the onion fermentation extract of the present invention inhibits the expression of HSP (Heat Shock Protein) 70, inhibits microvascular cell proliferation, inhibits microvascular cell migration and / or angiogenesis in skin cells, It is obvious that it is a material highly likely to be used as a composite material capable of preventing and / or improving skin irritation and / or skin damage such as hypertrophic scars, pruritus, redness, skin pigmentation,

Experimental Example  6: Confirmation of skin pigmentation inhibition by ultraviolet exposure

This clinical trial was made in reference to Korea Food & Drug Administration Notification No. 2009-130 (method and standard for measuring ultraviolet blocking effect) and was appropriately modified to suit the contents of the experiment.

(1) The test was carried out on the subject's arm, and the test site was selected as a clean place where skin damage, excessive hair, and hue were not particularly different.

(2) The minimum erythema amount was determined by referring to Fitzpatrick's skin type classification chart according to the skin condition of the subject, and skin coloration was induced in three skin areas of 1 cm to 1 cm in width UV irradiation was performed.

type Explanation MED (mJ / cm ² ) It is always easily (very badly) flushed and hardly blackened. 2 to 30 It is easily (badly) reddish and slightly black. 25 to 35 It is usually reddish and moderately black. 30 to 50 It does not get much red, it gets black easily. 45 to 60 It rarely turns red, and it becomes very black. 60 to 80 It does not become red at all and becomes very black. 85 ~ 200

(3) The ultraviolet rays were irradiated using an artificial solar irradiator equipped with a Xenon arc lamp having a continuous emission spectrum similar to that of the sunlight and having no specific peak. At this time, the ultraviolet range was from 290 to 400 nm including UV-B (290 to 320 nm) and UV-A (290 to 400 nm), and the wavelengths below 290 nm were removed by using a suitable filter.

(4) After irradiating the ultraviolet rays on the clean skin, one of the ultraviolet irradiated skin was coated with the ultraviolet screening product including the onion fermented extract prepared in Example 2, and the other was coated with the onion prepared in Comparative Example 4 The product was applied with the UV protection product excluding the fermentation extract, and the other one was not applied any UV protection product as a control.

(5) After UV irradiation, each cream was applied continuously for 10 days in order to exclude the influence of ultraviolet exposure in daily life. After 10 days, the degree of pigmentation of the subject's skin was measured with an Aramo TS skin meter (Manufactured by ARAM HUVIS Co., Ltd).

The results of skin pigmentation measurement of the subject after 10 days are shown in Figs. 7 to 13. A pigment was deposited on the basal layer of the skin using a 60 × magnification camera of the Aramo TS skin meter. Each measurement value indicates the area where the pigmentation occurred, and the larger the area value, the more extensive the pigmentation occurred.

Control group Comparative Example 4 Example 1 Subject 1 71 64 37 Subject 2 57 45 42 Subject 3 73 63 39 Subject 4 70 55 39 Subject 5 63 55 26 Subject 6 71 62 27 Subject 7 63 59 23

Table 3 is a table in which the degree of pigment deposition is numerically expressed. The numerical values are the results of measuring the degree of pigment deposition, and the degrees of pigment deposition and the measured values of the subjects as described above are shown in FIGS. 7 to 13 . As can be seen in Table 3, the ultraviolet screening agent containing the onion fermented extract of Example 2 was applied to a control group in which no ultraviolet screening agent was applied to the skin, and the ultraviolet screening agent excluding the onion skin fermentation extract of Comparative Example 4 It was confirmed that pigmentation was remarkably prevented or improved than that of the skin. It was confirmed that the pigmentation was remarkably prevented or improved even through the measurement photographs of FIGS. 7 to 13.

As described above, the onion fermentation extract of the present invention suppresses the expression of HSP (Heat Shock Protein) 70, inhibits microvascular cell proliferation, inhibits microvascular cell migration and / or angiogenesis in skin cells, A formulation capable of preventing or ameliorating skin irritation and / or skin damage, typically skin irritation, occurring in skin due to continuous exposure of ultraviolet rays by applying a cosmetic formulation for prevention and improvement of skin irritation caused by exposure, It is obvious that it is a material which is likely to be utilized.

[ Manufacturing example ]

Preparation Example 1: For the preparation of the cosmetic type for prevention and improvement of skin sensitization by UV exposure

The cosmetic formulation for prevention and improvement of skin hypersensitivity reaction caused by exposure to ultraviolet rays including the onion fermented extract of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically explained.

Manufacturing example  1-1. Cream with sunscreen (sunscreen)

Onion Fermented Extract 0.5 g

Glycerin 4 g

5 g of titanium oxide

Zinc oxide (zinc oxide) 2 g

To 5 g of ethylhexyl methoxycinnamate

4-methylbenzylidene camphor 3 g

Quaternium-18 Bentonite 0.9 g < RTI ID = 0.0 >

0.5 g of sodium chloride

Phytosterol (Phytosterol) 0.2 g

3 g of octocrylene

Dimethicone 3 g

0.5 g of preservative

Spices 0.5 g

Purified water 71.9 g

After mixing the ingredients, the ingredients were mixed according to the cream (sun protection) type product manufacturing method that included a conventional ultraviolet screening component. Alternatively, aluminum hydroxide or stearic acid may be mixed in place of titanium oxide, dimethicone may be mixed in place of zinc oxide, and dimethicone may be used instead of zinc oxide. Alternatively, a dimethicone / vinyl dimethicone crosspolymer may be mixed. Furthermore, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Manufacturing example  1-2. ample

Onion Fermented Extract 0.5 g

Dipropylene glycol (propylene glycol) 7 g

5 g of 1,3-butylene glycol (1,3-butylene glycol)

Polysorbate 80 (3 g)

Dipotassium glycyrrhizate 0.5 g < RTI ID = 0.0 >

Allantoin 0.5 g

0.5 g of panthenol

Glycerin 3 g

0.5 g of preservative

Spices 0.5 g

Purified water 79 g

After mixing the ingredients, the ingredients were mixed according to conventional ampoule product manufacturing methods. Furthermore, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Manufacturing example  1-3. skin

Onion Fermented Extract 0.5 g

5 g of 1,3-butylene glycol (1,3-butylene glycol)

Glycerin 4 g

Polysorbate 80 (2 g)

0.05 g of tocopherol acetate acetate

0.5 g of nicotinic acid amide

Dipotassium glycyrrhizate 0.5 g < RTI ID = 0.0 >

Allantoin 0.5 g

0.5 g of pyridoxine hydrochloride hydrochloride

0.5 g of preservative

Spices 0.5 g

Purified water 85.45 g

After mixing the ingredients, the ingredients were mixed according to the usual skin-type product manufacturing method. Furthermore, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Manufacturing example  1-4. pack

Onion Fermented Extract 0.5 g

5 g of glycerin

2 g of butylene glycol

Cellulose gum 1 g

0.2 g of carboxyvinylpolymer

1 g of polysorbate 80 (polysorbate 80)

Allantoin 0.3 g

0.5 g of panthenol

Arginine 0.3 g

40 g hydrogenated caster oil (PEG-40 hydrogenated caster oil)

Licorice extract 0.1 g

0.1 g of lecithin

0.5 g of preservative

Spices 0.5 g

Purified water 87.7 g

After mixing the components, the components were mixed according to a conventional pack-type product manufacturing method. Furthermore, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Manufacturing example  1-5. Lotion

Onion Fermented Extract 0.5 g

Glycerin 3 g

5 g of butylene glycol

Erythritol 1 g

0.3 g of carboxyvinylpolymer

0.3 g of xanthan gum

0.5 g of hydrogenated caster oil

0.5 g of glyceryl stearate

1 g of cetearyl alcohol

3 g of cetyl ethylhexanoate < RTI ID = 0.0 >

0.8 g of PEG-100 stearate (PEG-100 stearate)

0.2 g of arginine

Licorice extract 0.1 g

0.1 g of lecithin

0.5 g of preservative

Spices 0.5 g

Purified water 82.7 g

After mixing the ingredients, the ingredients were mixed according to conventional lotion-type product manufacturing methods. Furthermore, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Manufacturing example  1-6. essence

Onion Fermented Extract 0.5 g

Glycerin 3 g

5 g of butylene glycol

Erythritol 1 g

0.15 g of carboxyvinylpolymer

0.2 g of xanthan gum

0.5 g of glyceryl stearate

1 g of cetearyl alcohol

0.15 g of arginine

Licorice extract 0.1 g

0.1 g of lecithin

0.5 g of preservative

Spices 0.5 g

Purified water 87.3 g

After mixing the ingredients, the ingredients were mixed according to a conventional method of producing an essence-type product. Furthermore, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Claims (20)

A composition for preventing and improving skin hypersensitivity reaction by exposure to ultraviolet rays, which comprises an onion fermented extract.
The method according to claim 1,
A composition for the prevention and / or amelioration of a skin hypersensitivity reaction caused by exposure to ultraviolet ray, characterized by preventing and / or improving at least one selected from redness, skin pigmentation, skin pain, pruritus and hypertrophic scars occurring in the skin due to continuous exposure to ultraviolet rays.
The method according to claim 1,
Wherein the composition is used for at least one of a cosmetic, a medicine and a quasi-drug.
The method according to claim 1,
Wherein the concentration of the onion fermentation extract is 50 to 10,000 ppm.
5. The method of claim 4,
A composition for preventing or ameliorating a skin hypersensitivity reaction by exposure to ultraviolet radiation characterized by suppressing the expression of Heat Shock Protein (HSP) 70 in skin cells.
5. The method of claim 4,
A composition for preventing or ameliorating a skin hypersensitivity reaction by exposure to ultraviolet light, which inhibits proliferation or migration of skin cells.
5. The method of claim 4,
A composition for preventing or ameliorating a skin hypersensitivity reaction by exposure to ultraviolet light, characterized by suppressing vasodilation and angiogenesis of skin cells.
A cosmetic formulation for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet light, which comprises an onion fermented extract.
9. The method of claim 8,
Wherein the cosmetic composition is an emulsion, an emulsion, a cream, a lotion, an essence, a pack, a gel or a mist.
10. The method of claim 9,
But are not limited to, glycerin, titanium oxide, aluminum hydroxide, stearic acid, zinc oxide, dimethicone, ethylhexyl methoxycinnamate, 4-methylbenzylidene camphor, Quaternium-18 Bentonite, sodium chloride, phytosterol, octocrylene, and dimethicone / vinyl dimethicone Wherein the cosmetic composition further comprises one or more selected from the group consisting of dimethicone / vinyl dimethicone crosspolymer.
11. The method of claim 10,
To 100 parts by weight of an onion fermented extract, 700 to 900 parts by weight of glycerin; 900 to 1100 parts by weight of titanium oxide, aluminum hydroxide or stearic acid; 300-500 parts by weight of zinc oxide or dimethicone; 900 to 1100 parts by weight of ethylhexyl methoxycinnamate; 500 to 700 parts by weight of 4-methylbenzylidene camphor; 100-260 parts by weight of Quaternium-18 Bentonite; 50 to 150 parts by weight of sodium chloride; 20 to 60 parts by weight of phytosterol; 500 to 700 parts by weight of octocrylene; A cosmetic composition for prevention and improvement of skin hypersensitivity reaction by exposure to ultraviolet light, which comprises 500 to 700 parts by weight of dimethicone or dimethicone / vinyl dimethicone crosspolymer.
(1) preparing a medium containing dry pulverized onion pod;
(2) inoculating the medium with lactic acid bacteria to ferment the onions;
(3) extracting a culture medium containing fermented onion blood with a C ₁ -C ₄ alcohol solvent to obtain an alcohol solvent extract; And
(4) filtering the obtained alcohol solvent extract and drying to obtain an onion fermented extract;
≪ / RTI > wherein the fermented extract is a fermented onion.
13. The method of claim 12,
Wherein the medium of step (1) comprises 0.1 to 10% by weight of onion broth.
13. The method of claim 12,
The medium of step (1) comprises at least one selected from a carbon source, a nitrogen source, and trace elements,
The carbon source may be selected from the group consisting of glucose, sucrose, maltose, fructose, lactose, xylose, galactose and arabinose. Comprising at least one species,
Wherein the nitrogen source comprises at least one selected from the group consisting of yeast extract, peptone, beef extract,
The trace element may be selected from the group consisting of sodium acetate, potassium phosphate, diammonium citrate, magnesium sulfate (MgSO ₄ ), manganese sulfate (MnSO ₄ ), zinc sulfate , ZnSO ₄ , copper sulfate (CuSO ₄ ), and the like.
15. The method of claim 14,
The medium of step (1) comprises 0.01 to 10% by weight of onion, 0.1 to 5% by weight of glucose, 0.1 to 2% by weight of yeast extract, 0.01 to 0.2% by weight of potassium phosphate, 0.01 to 0.5% by weight of sodium acetate, 0.01 to 0.5% by weight of diammonium citrate, 0.001 to 0.01% by weight of magnesium sulfate (MgSO ₄ ), 0.001 to ₅ % by weight of manganese sulfate (MnSO ₄ ) 0.05% by weight of zinc sulfate, 0.0001 to 0.01% by weight of zinc sulfate (ZnSO ₄ ), 0.0001 to 0.01% by weight of copper sulfate (CuSO ₄ ) and the balance of purified water.
13. The method of claim 12,
Wherein the step (1) further comprises sterilizing the medium at 90 to 121 for 15 to 30 minutes.
13. The method of claim 12,
The lactic acid bacteria of step (2) may be selected from the group consisting of Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus brevis and Lactobacillus acidophilus (lactobacillus acidophilus). < RTI ID = 0.0 > 21. < / RTI >
18. The method of claim 17,
Wherein the inoculation amount of the lactic acid bacteria in the step (2) is 10 ⁵ to 10 ⁶ cfu / ml at the initial stage of inoculation.
13. The method of claim 12,
Wherein the alcohol solvent in step (3) is 20 to 90 wt% ethanol aqueous solution, and the extraction in step (3) is performed using the aqueous ethanol solution for 17 to 72 hours.
13. The method of claim 12,
The method according to any one of claims 1 to 3, wherein the alcoholic solvent extracted in step (4) is concentrated in vacuo at a temperature of 40 to 80 and then dried by vacuum drying, spray drying or freeze drying. A method for producing a fermented extract.

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